AU2023216497A1 - Mirror-image selection of l-nucleic acid aptamers - Google Patents

Mirror-image selection of l-nucleic acid aptamers Download PDF

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Publication number
AU2023216497A1
AU2023216497A1 AU2023216497A AU2023216497A AU2023216497A1 AU 2023216497 A1 AU2023216497 A1 AU 2023216497A1 AU 2023216497 A AU2023216497 A AU 2023216497A AU 2023216497 A AU2023216497 A AU 2023216497A AU 2023216497 A1 AU2023216497 A1 AU 2023216497A1
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AU
Australia
Prior art keywords
dna
nucleic acid
aptamer
aptamers
sequencing
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Pending
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AU2023216497A
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English (en)
Inventor
Ji Chen
Ting Zhu
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Tsinghua University
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Tsinghua University
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Publication of AU2023216497A1 publication Critical patent/AU2023216497A1/en
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1048—SELEX
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/50—Physical structure
    • C12N2310/53—Physical structure partially self-complementary or closed
    • C12N2310/531—Stem-loop; Hairpin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/30—Production chemically synthesised
    • C12N2330/31—Libraries, arrays

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  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AU2023216497A 2022-02-03 2023-02-02 Mirror-image selection of l-nucleic acid aptamers Pending AU2023216497A1 (en)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US202263306139P 2022-02-03 2022-02-03
US63/306,139 2022-02-03
US202263311092P 2022-02-17 2022-02-17
US63/311,092 2022-02-17
PCT/IB2023/050908 WO2023148646A1 (en) 2022-02-03 2023-02-02 Mirror-image selection of l-nucleic acid aptamers

Publications (1)

Publication Number Publication Date
AU2023216497A1 true AU2023216497A1 (en) 2024-08-15

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
AU2023216497A Pending AU2023216497A1 (en) 2022-02-03 2023-02-02 Mirror-image selection of l-nucleic acid aptamers

Country Status (7)

Country Link
US (1) US20250027090A1 (https=)
EP (1) EP4473115A1 (https=)
JP (1) JP2025507269A (https=)
CN (1) CN119013403A (https=)
AU (1) AU2023216497A1 (https=)
CA (1) CA3243286A1 (https=)
WO (1) WO2023148646A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN119757294B (zh) * 2024-12-20 2025-08-29 厦门市亮惠诊断技术有限公司 一种用于d-乳酸高灵敏检测方法

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4275149A (en) 1978-11-24 1981-06-23 Syva Company Macromolecular environment control in specific receptor assays
US4737456A (en) 1985-05-09 1988-04-12 Syntex (U.S.A.) Inc. Reducing interference in ligand-receptor binding assays
US20070141598A1 (en) * 2005-02-09 2007-06-21 Pacific Biosciences Of California, Inc. Nucleotide Compositions and Uses Thereof
WO2013170963A2 (en) 2012-05-16 2013-11-21 Noxxon Pharma Ag Enzymatic synthesis of l-nucleic acids
CN106467910A (zh) * 2015-08-18 2017-03-01 清华大学 L-dna/l-rna聚合酶及其应用
TWI731028B (zh) * 2016-01-22 2021-06-21 國立大學法人東京大學 用以篩選核酸適體之方法
WO2019166457A1 (en) * 2018-02-28 2019-09-06 F. Hoffmann-La Roche Ag Tagged nucleoside compounds useful for nanopore detection
CA3094598A1 (en) * 2018-04-05 2019-10-10 Tsinghua University Methods of sequencing and producing nucleic acid sequences
WO2019236571A1 (en) 2018-06-04 2019-12-12 Chan Zuckerberg Biohub, Inc. Compositions and methods involving non-natural aptamer libraries
CN111057132B (zh) * 2019-10-16 2022-01-07 四川农业大学 牛病毒性腹泻病毒e0蛋白氨基酸及制备方法

Also Published As

Publication number Publication date
JP2025507269A (ja) 2025-03-18
EP4473115A1 (en) 2024-12-11
US20250027090A1 (en) 2025-01-23
CA3243286A1 (en) 2023-08-10
CN119013403A (zh) 2024-11-22
WO2023148646A1 (en) 2023-08-10

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