CN118159646A - 产生定向心脏祖细胞的方法 - Google Patents

产生定向心脏祖细胞的方法 Download PDF

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Publication number
CN118159646A
CN118159646A CN202280071773.1A CN202280071773A CN118159646A CN 118159646 A CN118159646 A CN 118159646A CN 202280071773 A CN202280071773 A CN 202280071773A CN 118159646 A CN118159646 A CN 118159646A
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cells
population
cardiac
committed
positive
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CN202280071773.1A
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Chinese (zh)
Inventor
S·卡特曼
C·库恩斯
M·博伊尔
K·斯塔克
E·希伯伦
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Fujifilm Cell Power Co
Fujifilm Holdings America Corp
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Fujifilm Cell Power Co
Fujifilm Holdings America Corp
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    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
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    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
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    • A61K35/34—Muscles; Smooth muscle cells; Heart; Cardiac stem cells; Myoblasts; Myocytes; Cardiomyocytes
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CN202280071773.1A 2021-09-13 2022-09-13 产生定向心脏祖细胞的方法 Pending CN118159646A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202163243606P 2021-09-13 2021-09-13
US63/243,606 2021-09-13
PCT/US2022/076328 WO2023039588A1 (en) 2021-09-13 2022-09-13 Methods for the production of committed cardiac progenitor cells

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Publication Number Publication Date
CN118159646A true CN118159646A (zh) 2024-06-07

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CN202280071773.1A Pending CN118159646A (zh) 2021-09-13 2022-09-13 产生定向心脏祖细胞的方法

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US (1) US20230078230A1 (https=)
EP (1) EP4402249A1 (https=)
JP (1) JP2024531682A (https=)
KR (1) KR20240056604A (https=)
CN (1) CN118159646A (https=)
AU (1) AU2022343749A1 (https=)
CA (1) CA3231501A1 (https=)
WO (1) WO2023039588A1 (https=)

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2025117472A1 (en) * 2023-11-30 2025-06-05 Immorta Bio, Inc. Treatment of heart failure using pluripotent stem cell derived cardiomyocytes and derivatives thereof

Family Cites Families (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2197915B (en) 1986-11-19 1990-11-14 Rolls Royce Plc Improvements in or relating to fluid bearings
US6416998B1 (en) 1992-09-02 2002-07-09 Baylor College Of Medicine Plasmid encoding a modified steroid hormone
US5874301A (en) 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5763270A (en) 1995-06-07 1998-06-09 Genemedicine, Inc. Plasmid for delivery of nucleic acids to cells and methods of use
CA2277278A1 (en) 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US6099832A (en) 1997-05-28 2000-08-08 Genzyme Corporation Transplants for myocardial scars
US6280718B1 (en) 1999-11-08 2001-08-28 Wisconsin Alumni Reasearch Foundation Hematopoietic differentiation of human pluripotent embryonic stem cells
IL155728A0 (en) 2000-11-22 2003-11-23 Geron Corp Tolerizing allografts of pluripotent stem cells
PE20020958A1 (es) 2001-03-23 2002-11-14 Bayer Corp Inhibidores de la rho-quinasa
UY27224A1 (es) 2001-03-23 2002-10-31 Bayer Corp Inhibidores de la rho-quinasa
US20030211603A1 (en) 2001-08-14 2003-11-13 Earp David J. Reprogramming cells for enhanced differentiation capacity using pluripotent stem cells
IL162130A0 (en) 2001-12-07 2005-11-20 Robarts Res Inst Hematopoietic cells from human embryonic stem cells
JP4505228B2 (ja) 2002-01-10 2010-07-21 バイエル・シェーリング・ファルマ・アクチェンゲゼルシャフト Ｒｈｏ−キナーゼ阻害剤
EP1470121B1 (en) 2002-01-23 2012-07-11 Bayer HealthCare LLC Pyrimidine derivatives as rho-kinase inhibitors
MXPA04007196A (es) 2002-01-23 2005-06-08 Bayer Pharmaceuticals Corp Inhibidores de rho-quinasa.
AU2003263653A1 (en) 2002-09-27 2004-04-19 Daewoong Co., Ltd. A vaccine enhancing the protective immunity to hepatitis c virus using plasmid dna and recombinant adenovirus
ES2273047T3 (es) 2002-10-28 2007-05-01 Bayer Healthcare Ag Fenilaminopirimidinas sustituidas con heteroariloxi como inhibidores de rho-cinasa.
US7682828B2 (en) 2003-11-26 2010-03-23 Whitehead Institute For Biomedical Research Methods for reprogramming somatic cells
WO2005123902A1 (ja) 2004-06-18 2005-12-29 Riken 無血清浮遊培養による胚性幹細胞の神経分化誘導法
AU2005282414C1 (en) 2004-09-08 2011-04-07 Wisconsin Alumni Research Foundation Culturing human embryonic stem cells
EP3147296A1 (en) 2005-11-14 2017-03-29 Merial, Inc. Gene therapy for renal failure
US20070116680A1 (en) 2005-11-18 2007-05-24 Rensselaer Polytechnic Institute Stem cells within gel microenvironments
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
US8129187B2 (en) 2005-12-13 2012-03-06 Kyoto University Somatic cell reprogramming by retroviral vectors encoding Oct3/4. Klf4, c-Myc and Sox2
US8048999B2 (en) 2005-12-13 2011-11-01 Kyoto University Nuclear reprogramming factor
JP5813321B2 (ja) 2007-03-23 2015-11-17 ウィスコンシン アラムニ リサーチ ファンデーション 体細胞の再プログラム化
US9683232B2 (en) 2007-12-10 2017-06-20 Kyoto University Efficient method for nuclear reprogramming
EP3279314A1 (en) 2008-06-04 2018-02-07 Cellular Dynamics International, Inc. Methods for the production of ips cells using non-viral approach
AU2009274517B2 (en) * 2008-07-25 2015-03-26 The University Of Georgia Research Foundation, Inc. Compositions for Mesoderm derived ISL1+ Multipotent cells (IMPs), epicardial progenitor cells (EPCs) and multipotent CXCR4+CD56+ cells (C56Cs) and methods of use
CA2734128A1 (en) 2008-08-12 2010-02-18 Cellular Dynamics International, Inc. Methods for the production of ips cells
DK2356221T3 (en) 2008-10-24 2019-02-18 Wisconsin Alumni Res Found Pluripotent stem cells obtained by non-viral reprogramming
WO2010141801A2 (en) 2009-06-05 2010-12-09 Cellular Dynamics International, Inc. Reprogramming t cells and hematophietic cells
CA2770412C (en) 2009-08-07 2018-09-11 Kyoto University Method of efficiently establishing induced pluripotent stem cells
JP5902092B2 (ja) 2009-10-19 2016-04-13 セルラー ダイナミクス インターナショナル， インコーポレイテッド 心筋細胞の生成
WO2011056971A2 (en) 2009-11-04 2011-05-12 Cellular Dynamics International, Inc. Episomal reprogramming with chemicals
US9005967B2 (en) 2010-01-22 2015-04-14 Kyoto University Myc variants improve induced pluripotent stem cell generation efficiency
WO2011159684A2 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
US9884076B2 (en) * 2012-06-05 2018-02-06 Capricor, Inc. Optimized methods for generation of cardiac stem cells from cardiac tissue and their use in cardiac therapy
US20150337266A1 (en) * 2012-12-28 2015-11-26 Kyoto University Method for producing induced pluripotent stem cells, cardiomyocytes or precursor cells thereof
MA40589A (fr) * 2014-08-22 2017-06-28 Procella Therapeutics Ab Utilisation de jagged 1/frizzled 4 à titre de marqueurs de surface cellulaire pour isoler des cellules progénitrices ventriculaires cardiaques humaines
US9765299B2 (en) * 2014-09-10 2017-09-19 Wisconsin Alumni Research Foundation Chemically defined albumin-free conditions for cardiomyocyte differentiation of human pluripotent stem cells
CA3109078A1 (en) * 2018-07-17 2020-01-23 The Regents Of The University Of California Cells differentiated from immunoengineered pluripotent cells

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AU2022343749A1 (en) 2024-03-28
CA3231501A1 (en) 2023-03-16
KR20240056604A (ko) 2024-04-30
JP2024531682A (ja) 2024-08-29
EP4402249A1 (en) 2024-07-24
WO2023039588A1 (en) 2023-03-16
US20230078230A1 (en) 2023-03-16

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