CN118077894A - Fish scale glue-based nutritional supplement composition and preparation method thereof - Google Patents
Fish scale glue-based nutritional supplement composition and preparation method thereof Download PDFInfo
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- 239000000203 mixture Substances 0.000 title claims abstract description 36
- 235000015872 dietary supplement Nutrition 0.000 title claims abstract description 34
- 239000003292 glue Substances 0.000 title claims abstract description 16
- 238000002360 preparation method Methods 0.000 title claims abstract description 15
- QOSSAOTZNIDXMA-UHFFFAOYSA-N Dicylcohexylcarbodiimide Chemical compound C1CCCCC1N=C=NC1CCCCC1 QOSSAOTZNIDXMA-UHFFFAOYSA-N 0.000 claims abstract description 93
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 85
- 230000002929 anti-fatigue Effects 0.000 claims abstract description 69
- 241000251468 Actinopterygii Species 0.000 claims abstract description 67
- 239000000843 powder Substances 0.000 claims abstract description 54
- GUOCOOQWZHQBJI-UHFFFAOYSA-N 4-oct-7-enoxy-4-oxobutanoic acid Chemical compound OC(=O)CCC(=O)OCCCCCCC=C GUOCOOQWZHQBJI-UHFFFAOYSA-N 0.000 claims abstract description 52
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- 229920000178 Acrylic resin Polymers 0.000 claims abstract description 52
- 229940080313 sodium starch Drugs 0.000 claims abstract description 52
- 229920002472 Starch Polymers 0.000 claims abstract description 50
- 239000000243 solution Substances 0.000 claims abstract description 50
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- 239000000126 substance Substances 0.000 claims abstract description 38
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims abstract description 35
- 238000002156 mixing Methods 0.000 claims abstract description 24
- 239000003814 drug Substances 0.000 claims abstract description 20
- 238000000034 method Methods 0.000 claims abstract description 19
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- 235000008434 ginseng Nutrition 0.000 claims description 45
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 33
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 33
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- 239000002994 raw material Substances 0.000 claims description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 235000001014 amino acid Nutrition 0.000 claims description 15
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- 238000002791 soaking Methods 0.000 claims description 9
- 239000006228 supernatant Substances 0.000 claims description 7
- 108010010803 Gelatin Proteins 0.000 claims description 6
- 102000006995 beta-Glucosidase Human genes 0.000 claims description 6
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- 239000000706 filtrate Substances 0.000 claims description 6
- 239000008273 gelatin Substances 0.000 claims description 6
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- 235000011852 gelatine desserts Nutrition 0.000 claims description 6
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- 240000000171 Crataegus monogyna Species 0.000 claims description 4
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- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 claims description 4
- AGPKZVBTJJNPAG-WHFBIAKZSA-N L-isoleucine Chemical compound CC[C@H](C)[C@H](N)C(O)=O AGPKZVBTJJNPAG-WHFBIAKZSA-N 0.000 claims description 4
- BVHLGVCQOALMSV-JEDNCBNOSA-N L-lysine hydrochloride Chemical compound Cl.NCCCC[C@H](N)C(O)=O BVHLGVCQOALMSV-JEDNCBNOSA-N 0.000 claims description 4
- 235000003704 aspartic acid Nutrition 0.000 claims description 4
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 claims description 4
- 229960000310 isoleucine Drugs 0.000 claims description 4
- AGPKZVBTJJNPAG-UHFFFAOYSA-N isoleucine Natural products CCC(C)C(N)C(O)=O AGPKZVBTJJNPAG-UHFFFAOYSA-N 0.000 claims description 4
- 229960005337 lysine hydrochloride Drugs 0.000 claims description 4
- 244000303040 Glycyrrhiza glabra Species 0.000 claims description 3
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims description 3
- 244000042430 Rhodiola rosea Species 0.000 claims description 3
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- 244000273928 Zingiber officinale Species 0.000 claims description 3
- 235000006886 Zingiber officinale Nutrition 0.000 claims description 3
- 229960005261 aspartic acid Drugs 0.000 claims description 3
- 235000008397 ginger Nutrition 0.000 claims description 3
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 claims description 3
- 230000003301 hydrolyzing effect Effects 0.000 claims description 3
- 235000011477 liquorice Nutrition 0.000 claims description 3
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- 230000002255 enzymatic effect Effects 0.000 claims description 2
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- 239000000047 product Substances 0.000 claims description 2
- 150000003722 vitamin derivatives Chemical class 0.000 claims description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 18
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 18
- 235000011187 glycerol Nutrition 0.000 description 10
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 9
- 229930003268 Vitamin C Natural products 0.000 description 9
- RADKZDMFGJYCBB-UHFFFAOYSA-N pyridoxal hydrochloride Natural products CC1=NC=C(CO)C(C=O)=C1O RADKZDMFGJYCBB-UHFFFAOYSA-N 0.000 description 9
- 235000019158 vitamin B6 Nutrition 0.000 description 9
- 239000011726 vitamin B6 Substances 0.000 description 9
- 235000019154 vitamin C Nutrition 0.000 description 9
- 239000011718 vitamin C Substances 0.000 description 9
- 229940011671 vitamin b6 Drugs 0.000 description 9
- 238000010521 absorption reaction Methods 0.000 description 8
- 230000000052 comparative effect Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 210000002784 stomach Anatomy 0.000 description 6
- 230000009182 swimming Effects 0.000 description 6
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 4
- 102000008186 Collagen Human genes 0.000 description 3
- 108010035532 Collagen Proteins 0.000 description 3
- 229920001436 collagen Polymers 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 3
- 238000011049 filling Methods 0.000 description 3
- 210000002966 serum Anatomy 0.000 description 3
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- 239000004310 lactic acid Substances 0.000 description 2
- 235000014655 lactic acid Nutrition 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 210000000813 small intestine Anatomy 0.000 description 2
- 235000013826 starch sodium octenyl succinate Nutrition 0.000 description 2
- 239000001334 starch sodium octenyl succinate Substances 0.000 description 2
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- 238000013268 sustained release Methods 0.000 description 2
- 239000012730 sustained-release form Substances 0.000 description 2
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- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical class CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 description 1
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
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- 230000036449 good health Effects 0.000 description 1
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Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/16—Inorganic salts, minerals or trace elements
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/175—Amino acids
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Health & Medical Sciences (AREA)
- Nutrition Science (AREA)
- Inorganic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention relates to the field of nutritional supplement compositions, and particularly discloses a nutritional supplement composition based on fish scale glue and a preparation method thereof; the method comprises the following steps: s1: taking acrylic resin solution, water, starch, sodium starch octenyl succinate and N, N' -dicyclohexyl carbodiimide, heating to react, adding anti-fatigue nutrient substances, stirring, homogenizing and drying to obtain a quick-release component; s2: taking acrylic resin solution, starch, water, sodium starch octenyl succinate and N, N' -dicyclohexyl carbodiimide, heating to react, adding different parts of inner microcapsules, and stirring to obtain a slow-release component A and a slow-release component B; s3: mixing the quick-release component, the slow-release component A, the slow-release component B, the anti-fatigue traditional Chinese medicine powder, glycerol, poloxamer, vitamins and water to obtain the nutritional supplement composition.
Description
Technical Field
The invention relates to the field of nutritional supplement compositions, and particularly discloses a nutritional supplement composition based on fish scale glue and a preparation method thereof.
Background
The fish scale collagen has various effects including enhancing human immunity, promoting wound healing, maintaining beauty and keeping young, promoting health, resisting diseases and aging, improving sleep quality and the like, and can be used as a nutritional supplement composition to prepare an oral liquid with good health care effect, and can effectively improve health and relieve fatigue.
However, there are problems in this type of commercially available oral liquid: the collagen has the advantages of low absorption rate of human bodies, reduced effect caused by the destruction of nutrient components by gastric acid, difficult absorption caused by poor dispersibility, reduced absorption rate caused by the stimulation of the collagen to intestines and stomach, reduced absorption rate caused by uncontrolled release speed of nutrient substances, and the like. Therefore, it is of great importance to develop a nutritional supplement composition which is easy to absorb and which can maximize the beneficial effects of fish scale glue.
Disclosure of Invention
The invention aims to provide a nutritional supplement composition based on fish scale glue and a preparation method thereof, which are used for solving the problems in the background technology.
In order to solve the technical problems, the invention provides the following technical scheme:
a preparation method of a nutritional supplement composition based on fish scale gelatin comprises the following steps: the method comprises the following steps: s1: taking acrylic resin solution, starch, water, sodium starch octenyl succinate and N, N' -dicyclohexyl carbodiimide, heating to react, adding anti-fatigue nutrient substances, stirring, homogenizing and drying to obtain a quick-release component;
S2: taking acrylic resin solution, water, starch, sodium starch octenyl succinate, ginseng zymolyte and N, N' -dicyclohexyl carbodiimide, heating for reaction, adding anti-fatigue nutrient substances, stirring, homogenizing and drying; obtaining an inner layer microcapsule;
Taking acrylic resin solution, starch, water, sodium starch octenyl succinate and N, N' -dicyclohexyl carbodiimide, heating to react, adding the inner microcapsule, stirring for 20-30 min, homogenizing and drying to obtain a slow-release component A;
taking acrylic resin solution, starch, water, sodium starch octenyl succinate and N, N' -dicyclohexyl carbodiimide, heating to react, adding inner microcapsules, stirring for 50-60 min, homogenizing and drying to obtain a slow-release component B;
S3: mixing the quick-release component, the slow-release component A, the slow-release component B, the anti-fatigue traditional Chinese medicine powder, glycerol, poloxamer, vitamins and water to obtain the nutritional supplement composition.
Preferably, the quick-release component comprises the following raw materials in parts by weight: 400-500 parts of acrylic resin solution, 40-60 parts of starch, 20-30 parts of sodium starch octenyl succinate, 80-100 parts of water, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 20-30 parts of anti-fatigue nutrient substances;
the inner layer microcapsule comprises the following raw materials in parts by weight: 400-500 parts of acrylic resin solution, 60-80 parts of starch, 5-10 parts of sodium starch octenyl succinate, 80-100 parts of water, 10-20 parts of ginseng zymolyte, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 20-30 parts of anti-fatigue nutrient substances;
the slow-release component A comprises the following raw materials in parts by mass: 400-500 parts of acrylic resin solution, 40-60 parts of starch, 80-100 parts of water, 20-30 parts of sodium starch octenyl succinate, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 200 parts of inner layer microcapsules;
The slow-release component B comprises the following raw materials in parts by mass: 400-500 parts of acrylic resin solution, 40-60 parts of starch, 20-30 parts of sodium starch octenyl succinate, 80-100 parts of water, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 100 parts of inner layer microcapsules.
Preferably, the acrylic resin solution is ethanol comprising 15wt% acrylic resin No. i.
Preferably, the nutritional supplement composition comprises the following raw materials in parts by mass: 1 to 2 parts of quick-release component, 2 to 4 parts of slow-release component A, 2 to 4 parts of slow-release component B, 1 to 2 parts of glycerol, 5 to 8 parts of anti-fatigue traditional Chinese medicine powder, 0.1 to 0.2 part of poloxamer, 0.2 to 2 parts of vitamin and 100 parts of water.
Preferably, the vitamins comprise vitamin C and vitamin B6 in a mass ratio of 1:1;
preferably, the preparation of the anti-fatigue nutrient comprises the following steps: s1: mixing Ginseng radix powder, ethanol, water, and beta-glucosidase, stirring, adjusting pH, hydrolyzing, heating, extracting, centrifuging to obtain supernatant, and removing solvent to obtain Ginseng radix enzymolysis product;
S2: cleaning fish scales, sequentially soaking the fish scales in a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water, neutral protease and papain, heating for hydrolysis, filtering, concentrating, and freeze drying to obtain hydrolyzed fish scale gelatin powder;
S3: and (3) uniformly mixing the hydrolyzed fish scale gel powder, the ginseng enzymatic hydrolysate and the amino acid to obtain the anti-fatigue nutrient substance.
Preferably, the ginseng powder is prepared by the following steps: collecting Ginseng radix, cleaning, drying, cutting, vacuum freezing for 8 hr, drying, pulverizing, and sieving with 80 mesh sieve to obtain Ginseng radix powder.
Preferably, the fish scales are derived from fish scales of marine or freshwater fish.
Preferably, the fish scales are deep sea cod fish scales.
Preferably, the citric acid aqueous solution contains 15wt% of citric acid and the sodium hydroxide aqueous solution contains 10wt% of sodium hydroxide.
Preferably, the ginseng zymolyte comprises the following raw materials in parts by weight: 10-15 parts of ginseng powder, 40-60 parts of ethanol, 40-60 parts of water and 1-2 parts of beta-glucosidase; the hydrolyzed fish scale rubber powder comprises the following raw materials in parts by weight: 5 to 10 parts of fish scales, water with the mass 10 to 15 times of that of the fish scales, 0.5 to 1 part of neutral protease and 0.5 to 1 part of papain.
Preferably, in the anti-fatigue nutrient, the mass ratio of the hydrolyzed fish scale rubber powder to the ginseng zymolyte to the amino acid is (9-10): 2-3): 1-2; the amino acid comprises one or more of aspartic acid, isoleucine and lysine hydrochloride.
Preferably, the amino acids are aspartic acid, isoleucine, lysine hydrochloride in a mass ratio of 1:2:1.
Preferably, the preparation method of the ginseng zymolyte comprises the following steps: taking 12 parts of ginseng powder, 50 parts of ethanol, 50 parts of water and 2 parts of beta-glucosidase, stirring and adjusting the pH to 5, hydrolyzing under the action of a high-voltage pulse electric field with the electric field strength of 10kV/cm and the pulse number of 7, heating at 95 ℃ for 7min after the hydrolysis is finished, extracting with water saturated n-butanol, centrifuging, separating, taking supernatant, volatilizing to remove a solvent, and thus obtaining ginseng zymolyte.
Preferably, the preparation of the anti-fatigue traditional Chinese medicine powder comprises the following steps: decocting radix Rhodiolae, ginseng radix, rhizoma Polygonati, fructus crataegi, mume fructus, zingiberis rhizoma, and Glycyrrhrizae radix with water twice, filtering to obtain filtrate, mixing filtrates, standing, collecting supernatant, concentrating under reduced pressure, lyophilizing, and pulverizing to obtain antifatigue Chinese medicinal powder.
Preferably, the anti-fatigue traditional Chinese medicine powder comprises the following raw materials in parts by weight: 5 to 10 parts of rhodiola rosea, 5 to 10 parts of ginseng, 5 to 10 parts of rhizoma polygonati, 3 to 6 parts of hawthorn, 3 to 6 parts of dark plum, 1 to 3 parts of dried ginger and 1 to 2 parts of liquorice.
Preferably, the preparation of the anti-fatigue traditional Chinese medicine powder comprises the following steps: 8 parts of rhodiola rosea, 8 parts of ginseng, 5 parts of rhizoma polygonati, 5 parts of hawthorn, 3 parts of dark plum, 2 parts of dried ginger and 1 part of liquorice are taken, 100 parts of water is added for decoction twice at 90 ℃ for 2 hours each time, the filtrates are filtered, the filtrates are combined and kept stand for 24 hours, the supernatant is taken, the supernatant is concentrated into clear paste under reduced pressure, and the clear paste is frozen and dried and crushed into 100 meshes to obtain the anti-fatigue traditional Chinese medicine powder.
Compared with the prior art, the invention has the following beneficial effects: by adding enzyme, the hydrolyzed fish scale glue powder with smaller molecular weight is prepared and is easier to absorb; mixing the hydrolyzed fish scale gelatin powder, ginseng zymolyte and amino acid according to a certain proportion to obtain an anti-fatigue nutrient substance; in order to ensure that the anti-fatigue nutrient is not damaged by gastric acid and exert the effect to the maximum extent, the anti-fatigue nutrient is coated;
The anti-fatigue nutrient is coated by acrylic resin solution, starch and sodium starch octenyl succinate to obtain quick-release components, and the quick-release components can start to release the anti-fatigue nutrient after entering the small intestine, wherein ginseng zymolyte contained in the anti-fatigue nutrient has a certain anti-inflammatory capability besides the anti-fatigue and anti-oxidation effects, and can relieve the stimulation possibly caused by absorbing hydrolyzed fish scale gelatin powder and promote absorption; the sodium starch octenyl succinate enhances the dispersibility among particles of the quick-release component, so that the quick-release component is easier to absorb;
Coating an anti-fatigue nutrient substance with an acrylic resin solution, starch, sodium starch octenyl succinate and ginseng zymolyte to obtain an inner layer microcapsule; coating the inner layer microcapsule with acrylic resin solution, starch and sodium starch octenyl succinate, and controlling stirring time to obtain a slow-release component A and a slow-release component B; the ginseng zymolyte is also added into the inner microcapsule shell layer to promote the absorption of hydrolyzed fish scale gelatin powder; the adding amount of the sodium starch octenyl succinate in the inner microcapsule needs to be controlled, and too much starch sodium octenyl succinate can cause difficulty in recoating, and too little starch sodium octenyl succinate can cause agglomeration and influence absorption; due to different preparation processes, the embedding amounts of the slow-release component A and the slow-release component B are different, and the release time is also different, so that the slow-release component A and the slow-release component B are matched with the quick-release component for use, the release time of the medicine can be controlled, and the absorbability is improved; the quick-release component is added in a small amount, so that the small intestine is gradually adapted, the possible irritation is reduced, and then the slow-release component A and the slow-release component B slowly release the rest anti-fatigue nutrient substances, which are matched according to a certain proportion for use, so that the absorption is facilitated;
The anti-fatigue traditional Chinese medicine powder is added, so that the nutrition and the anti-fatigue capability are further improved, wherein the hawthorn and the dark plum can improve the overall taste.
Detailed Description
What follows is a preferred implementation of the embodiments of the invention, it being apparent that the described embodiments are only some, but not all, of the embodiments of the invention. All other embodiments, which are apparent to those of ordinary skill in the art without undue burden, are within the scope of the invention, as would be within the skill of one of ordinary skill in the art without departing from the principles of the embodiments of the present invention.
The following parts are mass parts unless specified;
Example 1: s1: cleaning 10 parts of fish scales, sequentially soaking for 24 hours by using a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water 15 times of the mass of the fish scales, adding 1 part of neutral protease and 0.5 part of papain, heating to 50 ℃, preserving heat for 5 hours for hydrolysis, filtering, concentrating, and freeze-drying to obtain hydrolyzed fish scale glue powder;
s2: taking hydrolyzed fish scale rubber powder, ginseng zymolyte and amino acid according to the mass ratio of 10:2:1, and uniformly mixing to obtain an anti-fatigue nutrient substance;
S3: taking 400 parts of acrylic resin solution, adding 50 parts of starch, 25 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a quick-release component;
S4: taking 400 parts of acrylic resin solution, adding 70 parts of starch, 8 parts of sodium starch octenyl succinate, 100 parts of water, 15 parts of ginseng zymolyte and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying; obtaining an inner layer microcapsule;
taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 200 parts of inner layer microcapsules, stirring for 25min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component A;
Taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 100 parts of inner layer microcapsules, stirring for 55min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component B;
s5: mixing 1 part of quick-release component, 3 parts of slow-release component A, 2 parts of slow-release component B, 6 parts of anti-fatigue traditional Chinese medicine powder, 1 part of glycerin, 0.1 part of poloxamer, 0.4 part of vitamin C, 0.4 part of vitamin B6 and 100 parts of water to obtain the nutritional supplement composition.
Example 2: s1: cleaning 10 parts of fish scales, sequentially soaking for 24 hours by using a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water 15 times of the mass of the fish scales, adding 1 part of neutral protease and 0.5 part of papain, heating to 50 ℃, preserving heat for 5 hours for hydrolysis, filtering, concentrating, and freeze-drying to obtain hydrolyzed fish scale glue powder;
s2: taking hydrolyzed fish scale rubber powder, ginseng zymolyte and amino acid according to the mass ratio of 10:2:1, and uniformly mixing to obtain an anti-fatigue nutrient substance;
S3: taking 500 parts of acrylic resin solution, adding 60 parts of starch, 30 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 30 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a quick-release component;
s4: taking 500 parts of acrylic resin solution, adding 80 parts of starch, 10 parts of sodium starch octenyl succinate, 100 parts of water, 20 parts of ginseng zymolyte and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 30 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying; obtaining an inner layer microcapsule;
taking 500 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 30 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 200 parts of inner layer microcapsules, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component A;
Taking 500 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 30 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 100 parts of inner layer microcapsules, stirring for 60 minutes, homogenizing for 8 minutes at 160 r/min, and drying to obtain a slow-release component B;
S5: mixing 2 parts of quick-release component, 2 parts of slow-release component A, 4 parts of slow-release component B, 6 parts of anti-fatigue traditional Chinese medicine powder, 1 part of glycerol, 0.1 part of poloxamer, 0.4 part of vitamin C, 0.4 part of vitamin B6 and 100 parts of water to obtain the nutritional supplement composition.
Example 3: s1: cleaning 10 parts of fish scales, sequentially soaking for 24 hours by using a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water 15 times of the mass of the fish scales, adding 1 part of neutral protease and 0.5 part of papain, heating to 50 ℃, preserving heat for 5 hours for hydrolysis, filtering, concentrating, and freeze-drying to obtain hydrolyzed fish scale glue powder;
s2: taking hydrolyzed fish scale rubber powder, ginseng zymolyte and amino acid according to the mass ratio of 10:2:1, and uniformly mixing to obtain an anti-fatigue nutrient substance;
s3: taking 400 parts of acrylic resin solution, adding 40 parts of starch, 20 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 20 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a quick-release component;
S4: taking 400 parts of acrylic resin solution, adding 60 parts of starch, 5 parts of sodium starch octenyl succinate, 100 parts of water, 10 parts of ginseng zymolyte and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 20 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying; obtaining an inner layer microcapsule;
taking 400 parts of acrylic resin solution, adding 40 parts of starch, 100 parts of water, 20 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 200 parts of inner layer microcapsules, stirring for 20min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component A;
400 parts of acrylic resin solution is taken, 400 parts of starch, 100 parts of water, 20 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide are added, the temperature is raised to 60 ℃ for reaction for 2 hours, 100 parts of inner layer microcapsules are added, stirring is carried out for 50-60 min, homogenization is carried out for 8min at 160 r/min, and the slow-release component B is obtained after drying;
s5: mixing 1 part of quick-release component, 4 parts of slow-release component A, 2 parts of slow-release component B, 6 parts of anti-fatigue traditional Chinese medicine powder, 1 part of glycerin, 0.1 part of poloxamer, 0.4 part of vitamin C, 0.4 part of vitamin B6 and 100 parts of water to obtain the nutritional supplement composition.
Comparative example 1 (varying the amounts of the quick-release component, the slow-release component a, and the slow-release component B added, the remaining process steps are the same as in example 1): s1: cleaning 10 parts of fish scales, sequentially soaking for 24 hours by using a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water 15 times of the mass of the fish scales, adding 1 part of neutral protease and 0.5 part of papain, heating to 50 ℃, preserving heat for 5 hours for hydrolysis, filtering, concentrating, and freeze-drying to obtain hydrolyzed fish scale glue powder;
s2: taking hydrolyzed fish scale rubber powder, ginseng zymolyte and amino acid according to the mass ratio of 10:2:1, and uniformly mixing to obtain an anti-fatigue nutrient substance;
S3: taking 400 parts of acrylic resin solution, adding 50 parts of starch, 25 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a quick-release component;
S4: taking 400 parts of acrylic resin solution, adding 70 parts of starch, 8 parts of sodium starch octenyl succinate, 100 parts of water, 15 parts of ginseng zymolyte and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying; obtaining an inner layer microcapsule;
taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 200 parts of inner layer microcapsules, stirring for 25min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component A;
Taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 100 parts of inner layer microcapsules, stirring for 55min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component B;
S5: mixing 4 parts of quick-release component, 2 parts of slow-release component A, 0.5 part of slow-release component B, 6 parts of anti-fatigue traditional Chinese medicine powder, 1 part of glycerol, 0.1 part of poloxamer, 0.4 part of vitamin C, 0.4 part of vitamin B6 and 100 parts of water to obtain the nutritional supplement composition.
Comparative example 2 (no ginseng matrix was added to the inner microcapsule, and the remaining process steps were identical to example 1): s1: cleaning 10 parts of fish scales, sequentially soaking for 24 hours by using a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water 15 times of the mass of the fish scales, adding 1 part of neutral protease and 0.5 part of papain, heating to 50 ℃, preserving heat for 5 hours for hydrolysis, filtering, concentrating, and freeze-drying to obtain hydrolyzed fish scale glue powder;
s2: taking hydrolyzed fish scale rubber powder, ginseng zymolyte and amino acid according to the mass ratio of 10:2:1, and uniformly mixing to obtain an anti-fatigue nutrient substance;
S3: taking 400 parts of acrylic resin solution, adding 50 parts of starch, 25 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a quick-release component;
S4: taking 400 parts of acrylic resin solution, adding 70 parts of starch, 8 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying; obtaining an inner layer microcapsule;
taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 200 parts of inner layer microcapsules, stirring for 25min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component A;
Taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 100 parts of inner layer microcapsules, stirring for 55min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component B;
s5: mixing 1 part of quick-release component, 3 parts of slow-release component A, 2 parts of slow-release component B, 6 parts of anti-fatigue traditional Chinese medicine powder, 1 part of glycerin, 0.1 part of poloxamer, 0.4 part of vitamin C, 0.4 part of vitamin B6 and 100 parts of water to obtain the nutritional supplement composition.
Comparative example 3 (varying the amounts of acrylic resin solution, starch, sodium starch octenyl succinate added, the remaining process steps being identical to those of example 1): s1: cleaning 10 parts of fish scales, sequentially soaking for 24 hours by using a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water 15 times of the mass of the fish scales, adding 1 part of neutral protease and 0.5 part of papain, heating to 50 ℃, preserving heat for 5 hours for hydrolysis, filtering, concentrating, and freeze-drying to obtain hydrolyzed fish scale glue powder;
s2: taking hydrolyzed fish scale rubber powder, ginseng zymolyte and amino acid according to the mass ratio of 10:2:1, and uniformly mixing to obtain an anti-fatigue nutrient substance;
S3: taking 400 parts of acrylic resin solution, adding 70 parts of starch, 8 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a quick-release component;
S4: taking 400 parts of acrylic resin solution, adding 50 parts of starch, 25 parts of sodium starch octenyl succinate, 100 parts of water, 15 parts of ginseng zymolyte and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying; obtaining an inner layer microcapsule;
Taking 400 parts of acrylic resin solution, adding 70 parts of starch, 100 parts of water, 8 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 200 parts of inner layer microcapsules, stirring for 25min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component A;
taking 400 parts of acrylic resin solution, adding 70 parts of starch, 100 parts of water, 8 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 100 parts of inner layer microcapsules, stirring for 55min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component B;
s5: mixing 1 part of quick-release component, 3 parts of slow-release component A, 2 parts of slow-release component B, 6 parts of anti-fatigue traditional Chinese medicine powder, 1 part of glycerin, 0.1 part of poloxamer, 0.4 part of vitamin C, 0.4 part of vitamin B6 and 100 parts of water to obtain the nutritional supplement composition.
Comparative example 4 (modified preparation method of sustained-release component a and sustained-release component B, and the remaining method steps are the same as in example 1): s1: cleaning 10 parts of fish scales, sequentially soaking for 24 hours by using a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water 15 times of the mass of the fish scales, adding 1 part of neutral protease and 0.5 part of papain, heating to 50 ℃, preserving heat for 5 hours for hydrolysis, filtering, concentrating, and freeze-drying to obtain hydrolyzed fish scale glue powder;
s2: taking hydrolyzed fish scale rubber powder, ginseng zymolyte and amino acid according to the mass ratio of 10:2:1, and uniformly mixing to obtain an anti-fatigue nutrient substance;
S3: taking 400 parts of acrylic resin solution, adding 50 parts of starch, 25 parts of sodium starch octenyl succinate, 100 parts of water and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying to obtain a quick-release component;
S4: taking 400 parts of acrylic resin solution, adding 70 parts of starch, 8 parts of sodium starch octenyl succinate, 100 parts of water, 15 parts of ginseng zymolyte and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 25 parts of anti-fatigue nutrient substances, stirring for 30min, homogenizing for 8min at 160 r/min, and drying; obtaining an inner layer microcapsule;
Taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 150 parts of inner layer microcapsules, stirring for 15min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component A;
Taking 400 parts of acrylic resin solution, adding 60 parts of starch, 100 parts of water, 25 parts of sodium starch octenyl succinate and 1 part of N, N' -dicyclohexylcarbodiimide, heating to 60 ℃ for reaction for 2 hours, adding 100 parts of inner layer microcapsules, stirring for 65min, homogenizing for 8min at 160 r/min, and drying to obtain a slow-release component B;
s5: mixing 1 part of quick-release component, 3 parts of slow-release component A, 2 parts of slow-release component B, 6 parts of anti-fatigue traditional Chinese medicine powder, 1 part of glycerin, 0.1 part of poloxamer, 0.4 part of vitamin C, 0.4 part of vitamin B6 and 100 parts of water to obtain the nutritional supplement composition.
In the above examples, the test methods used, unless otherwise specified, were all conventional; all the raw materials used, unless specified, are available from commercial sources as follows: ethanol (CAS: 64-17-5); beta-glucosidase (S10047, shanghai source leaf); neutral protease (S25046, shanghai-derived leaf); papain (S10011, shanghai-derived leaves); aspartic acid (WD 2375, a company of chemical industry, hubei wande); isoleucine (S20042, shanghai-derived leaves); lysine hydrochloride (GA 7750, guangdong biotechnology limited, hubei); acrylic resin number I (180109085929, shanghai Koehne Raman reagent Co., ltd.); starch (S11004, shanghai source leaf); sodium starch octenyl succinate (S25500, shanghai source leaf); n, N' -dicyclohexyl-carbodiimide (CAS: 538-75-0); glycerol (CAS: 56-81-5); poloxamer (poloxamer 188, basf); vitamin C (S13001, shanghai source leaf); vitamin B6 (S13026, shanghai source leaf).
Experiment: the nutritional supplement compositions prepared in examples 1 to 3 and comparative examples 1 to 4 were tested for anti-fatigue efficacy (on the premise that the results of the experiments in the behaviours were positive, any two of three indexes BLA, BUN, MG/LG were positive, and the formulation was judged to have the efficacy of relieving physical fatigue) by referring to the "evaluation method for health food function 2020 edition":
(1) Measuring the swimming time of the negative gravity exhaust: taking a nutrition supplementing composition, and carrying out stomach filling on a mouse, wherein the stomach filling amount is 0.1L/kg (body weight) of the mouse per day, the stomach filling is carried out continuously for 30 days, after the administration for 30min on the 30 th day, the mouse with the tail part bearing 5% of weight of lead skin is placed into a swimming box for swimming, the water depth is not less than 30cm, the water temperature is 28+/-1 ℃, the time from the beginning of the swimming of the mouse to the exhaustion of the water is recorded, the sinking of the mouse into the water is maintained, the water cannot float out of the water for 8 seconds, and the limbs cannot coordinate the water drawing time; (2) After the patient has a 30-min rest for 10min without load swimming after 38 days of gastric lavage, the eyeball is taken out to obtain blood, serum is separated, and BLA (blood lactic acid) is detected according to the instructions of a serum lactic acid test box; (3) After the non-load swimming is carried out for 90min and 60min for rest on day 38 of the intragastric administration, the eyeballs are picked up to obtain blood, serum is separated, and BUN (blood urea nitrogen) is measured in a tissue in a full-automatic biochemical analyzer according to the description of the kit;
taking distilled water, and pouring the stomach into the mice with the stomach pouring amount of 0.1L/kg (body weight) per day, and performing the experiment as comparative example 5; specific data are shown in the following table;
Conclusion: as can be seen from the above table, the nutritional supplement composition prepared by the invention has anti-fatigue effect; the proportion and the addition amount of the raw materials have important significance by comparison.
Finally, it should be noted that: the foregoing description of the preferred embodiments of the present application is merely illustrative, and the scope of the present application is not limited thereto, since any changes or substitutions that would be easily contemplated by those skilled in the art within the scope of the present application shall fall within the scope of the present application; the embodiments of the present application and features in the embodiments may be combined with each other without conflict. Therefore, the protection scope of the application is subject to the protection scope of the claims.
Claims (9)
1. A preparation method of a nutritional supplement composition based on fish scale glue is characterized by comprising the following steps: the method comprises the following steps: s1: taking acrylic resin solution, water, starch, sodium starch octenyl succinate and N, N' -dicyclohexyl carbodiimide, heating to react, adding anti-fatigue nutrient substances, stirring, homogenizing and drying to obtain a quick-release component;
S2: taking acrylic resin solution, water, starch, sodium starch octenyl succinate, ginseng zymolyte and N, N' -dicyclohexyl carbodiimide, heating for reaction, adding anti-fatigue nutrient substances, stirring, homogenizing and drying; obtaining an inner layer microcapsule;
Taking acrylic resin solution, starch, water, sodium starch octenyl succinate and N, N' -dicyclohexyl carbodiimide, heating to react, adding different parts of inner microcapsules, stirring, homogenizing and drying to obtain a slow-release component A and a slow-release component B respectively;
S3: mixing the quick-release component, the slow-release component A, the slow-release component B, the anti-fatigue traditional Chinese medicine powder, glycerol, poloxamer, vitamins and water to obtain the nutritional supplement composition.
2. The method for preparing a fish scale gel-based nutritional supplement composition according to claim 1, wherein the method comprises the steps of: the quick-release component comprises the following raw materials in parts by mass: 400-500 parts of acrylic resin solution, 40-60 parts of starch, 20-30 parts of sodium starch octenyl succinate, 80-100 parts of water, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 20-30 parts of anti-fatigue nutrient substances;
the inner layer microcapsule comprises the following raw materials in parts by weight: 400-500 parts of acrylic resin solution, 60-80 parts of starch, 5-10 parts of sodium starch octenyl succinate, 80-100 parts of water, 10-20 parts of ginseng zymolyte, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 20-30 parts of anti-fatigue nutrient substances;
The slow-release component A comprises the following raw materials in parts by mass: 400-500 parts of acrylic resin solution, 40-60 parts of starch, 20-30 parts of sodium starch octenyl succinate, 80-100 parts of water, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 200 parts of inner microcapsules;
The slow-release component B comprises the following raw materials in parts by mass: 400-500 parts of acrylic resin solution, 40-60 parts of starch, 20-30 parts of sodium starch octenyl succinate, 80-100 parts of water, 0.5-1.5 parts of N, N' -dicyclohexylcarbodiimide and 100 parts of inner layer microcapsules.
3. The method for preparing a fish scale gel-based nutritional supplement composition according to claim 1, wherein the method comprises the steps of: the nutritional supplement composition comprises the following raw materials in parts by mass: 1 to 2 parts of quick-release component, 2 to 4 parts of slow-release component A, 2 to 4 parts of slow-release component B, 1 to 2 parts of glycerol, 5 to 8 parts of anti-fatigue traditional Chinese medicine powder, 0.1 to 0.2 part of poloxamer, 0.2 to 2 parts of vitamin and 100 parts of water.
4. The method for preparing a fish scale gel-based nutritional supplement composition according to claim 1, wherein the method comprises the steps of: the preparation of the anti-fatigue nutrient comprises the following steps: s1: mixing Ginseng radix powder, ethanol, water, and beta-glucosidase, stirring, adjusting pH, hydrolyzing, heating, extracting, centrifuging to obtain supernatant, and removing solvent to obtain Ginseng radix enzymolysis product;
S2: cleaning fish scales, sequentially soaking the fish scales in a citric acid aqueous solution and a sodium hydroxide aqueous solution, cleaning and drying; adding water, neutral protease and papain, heating for hydrolysis, filtering, concentrating, and freeze drying to obtain hydrolyzed fish scale gelatin powder;
S3: and (3) uniformly mixing the hydrolyzed fish scale gel powder, the ginseng enzymatic hydrolysate and the amino acid to obtain the anti-fatigue nutrient substance.
5. The method of preparing a fish scale gel-based nutritional supplement composition according to claim 4, wherein: the ginseng zymolyte comprises the following raw materials in parts by weight: 10-15 parts of ginseng powder, 40-60 parts of ethanol, 40-60 parts of water and 1-2 parts of beta-glucosidase; the hydrolyzed fish scale rubber powder comprises the following raw materials in parts by weight: 5 to 10 parts of fish scales, water with the mass 10 to 15 times of that of the fish scales, 0.5 to 1 part of neutral protease and 0.5 to 1 part of papain.
6. The method of preparing a fish scale gel-based nutritional supplement composition according to claim 4, wherein: in the anti-fatigue nutrient substance, the mass ratio of the hydrolyzed fish scale rubber powder to the ginseng zymolyte to the amino acid is (9-10): 2-3): 1-2; the amino acid comprises one or more of aspartic acid, isoleucine and lysine hydrochloride.
7. The method for preparing a fish scale gel-based nutritional supplement composition according to claim 1, wherein the method comprises the steps of: the preparation method of the anti-fatigue traditional Chinese medicine powder comprises the following steps: decocting radix Rhodiolae, ginseng radix, rhizoma Polygonati, fructus crataegi, mume fructus, zingiberis rhizoma, and Glycyrrhrizae radix with water twice, filtering to obtain filtrate, mixing filtrates, standing, collecting supernatant, concentrating under reduced pressure, lyophilizing, and pulverizing to obtain antifatigue Chinese medicinal powder.
8. The method of preparing a fish scale gel-based nutritional supplement composition of claim 7, wherein: the anti-fatigue traditional Chinese medicine powder comprises the following raw materials in parts by weight: 5 to 10 parts of rhodiola rosea, 5 to 10 parts of ginseng, 5 to 10 parts of rhizoma polygonati, 3 to 6 parts of hawthorn, 3 to 6 parts of dark plum, 1 to 3 parts of dried ginger and 1 to 2 parts of liquorice.
9. A nutritional supplement composition prepared by the method of preparing a nutritional supplement composition based on fish scale glue according to any one of claims 1-8.
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| CN202410450581.2A CN118077894A (en) | 2024-04-15 | 2024-04-15 | Fish scale glue-based nutritional supplement composition and preparation method thereof |
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Application publication date: 20240528 |