CN118021647A - Cosmetic skin care mask containing hirudin and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of cosmetic processing, and particularly relates to a cosmetic skin care mask containing hirudin and a preparation method thereof. Preparing a part of auxiliary materials and active ingredients into a solution, immersing the mask cloth into the solution, performing primary freeze drying, dispersing hirudin in the solution of xanthan gum and glycerin, spraying the solution into the surface of the primary freeze-dried mask, performing secondary freeze drying, bagging and sterilizing to obtain the cosmetic skin care mask containing hirudin. According to the invention, by utilizing the characteristic that the xanthan gum has better solubility in both cold water and hot water, the xanthan gum can be dissolved in glycerin in the mask, and the hirudin has the characteristic of thermal stability in an environment without moisture, so that the hirudin is uniformly dispersed in an anhydrous glycerin and xanthan gum system, the problem that the hirudin is partially deactivated when the hirudin is freeze-dried in a humid environment is solved, and meanwhile, the problem that the hirudin is difficult to dissolve when the mask is used is avoided; screening out Ginseng radix and rhizoma Bletillae with synergistic effect with hirudin, and enhancing skin caring effect of facial mask.

Description

Cosmetic skin care mask containing hirudin and preparation method thereof

Technical Field

The invention belongs to the technical field of cosmetic processing, and particularly relates to a cosmetic skin care mask containing hirudin and a preparation method thereof.

Background

Masks were used in medicine for the treatment of skin disorders at the earliest and were gradually used later for cleansing and maintenance of the face. In recent years, with the improvement of the living standard of people, the consumption concept is changed, the mask is more and more popular with consumers, the use population of the mask is expanded from female to male, and the sales volume of the mask is increased in a blowout type.

The application mask is the most direct way of beautifying and protecting skin, and has the effects of whitening, moisturizing, promoting circulation, tightening skin and the like. The facial mask is used as a carrier of a beauty skin care product, and can be applied to the face and other parts to isolate the face skin from air, so that the temperature of the surface of the skin is increased, the blood circulation of the face is quickened, pores are opened, sweat glands are quickened to excrete sweat, waste is promoted to be discharged, the effect of cleaning cells is achieved, meanwhile, the beauty skin care substance in the facial mask permeates into the skin, more nutrients are brought to the skin, deep cells of the skin are nourished, the skin aging is delayed, the generation of wrinkles is restrained, and the whitening effect is achieved. The facial mask is applied on the face, and moisture in the facial mask can permeate into skin surface cells to enable the skin to fully absorb the moisture so as to achieve the moisturizing effect. When the facial mask is applied on the face, after the facial cells fully absorb water, the volume becomes large, so that the skin becomes shiny, and meanwhile, pores shrink, the skin can be moderately tightened, a tightening sense is generated, the skin is tightened and shrunk, wrinkles become shallow and fade, and the skin becomes more elastic.

The hirudin is a main active ingredient contained in leech, is a polypeptide containing 65 amino acids, has a molecular weight of 7000, is white or off-white, is very soluble in water, is water-soluble and poor in fat-solubility, is stable in a dry state, can exist stably in water for 6 months at room temperature, is extremely stable under the conditions of temperature change and acidic environment, is sensitive to humidity, is extremely stable under the condition that the humidity is below 10%, is increased along with the humidity when the humidity is above 10%, and gradually shortens the stabilizing time of the hirudin, and is unstable in a high-humidity environment due to the large water content of the mask, so that the hirudin becomes difficult to be used in the mask.

By searching, the prior art uses hirudin for the presence of a mask: CN1732897a discloses a leech extract beauty skin care liquid and a preparation method thereof, which is prepared from leech extract, auxiliary materials and clean water, and has two characteristics: firstly, the skin is self-penetrating, the skin is quickly absorbed, and the skin is anti-inflammatory and detumescent, and has unique effects on preventing skin aging, eliminating color spots, acne and new and old scars; secondly, the use is convenient, the cream is more suitable for being used in hot seasons, and the cream is more suitable for people with greasy skin. CN102188360a discloses a recombinant hirudin eye mask formulation and a preparation method thereof, wherein the raw materials comprise recombinant hirudin, jojoba oil, aloe oil, antioxidant and essence, and the preparation method comprises the following steps: uniformly mixing the components, standing, filtering, and dip-coating the filtrate on a non-woven fabric mask; CN110302133a discloses a regeneration repairing mask liquid using leech mucus extract and a preparation method thereof, raw materials are: glycerin, oleum Trigonellae, acrylic acid ester/C10-30 alkanol acrylate cross-linked polymer, hirudo mucus extract, herba Portulacae extract, ulmus pumila root extract, and oleum Anisi Stellati. The preparation method comprises the following steps: heating the component A until the component A is completely dissolved; mixing the raw materials of the component B, adding the mixed component C after the raw materials are transparent, and uniformly stirring until the raw materials are semi-permeable paste; and (3) adding the component D, stirring uniformly, defoaming, and discharging. The mask disclosed in the patent is a liquid moist environment, the temperature is higher than room temperature during processing, hirudin is unstable in the environment with the humidity higher than 10%, and the hirudin is easy to inactivate, so that the efficacy of the mask is affected.

CN117297996a discloses a freeze-dried hirudin mask and a preparation method thereof, which overcomes the defects that hirudin is unstable and easy to inactivate in water, and has bad smell at normal temperature; however, the preparation method comprises the steps of firstly mixing the hirudin extract with distilled water, placing the hirudin in an environment with high humidity, adding auxiliary materials, freeze-drying, and inactivating part of the hirudin at high temperature in the freeze-drying sublimation stage, wherein the mask has single efficacy, and the synergistic cooperation of other whitening skin care raw materials and the hirudin is not studied.

According to the application, on the basis of CN117297996A, the characteristic that xanthan gum has better solubility in hot and cold water is utilized, the xanthan gum can be dissolved in glycerin in the mask, the hirudin has the characteristic of thermal stability in a moisture-free environment, other components are creatively freeze-dried to prepare a semi-finished mask 1, the hirudin is uniformly dispersed in a moisture-free glycerin and xanthan gum system and then sprayed on the semi-finished mask 1, and the semi-finished mask is freeze-dried for the second time to prepare the skin care mask containing the hirudin, so that the problem that the hirudin is dissolved in water firstly in the patent CN117297996A, namely, the hirudin is generally deactivated in the freeze-drying sublimation stage in the analysis of the environment which is larger than room temperature is solved, and the problem that the hirudin is difficult to dissolve out in the use of the xanthan gum is avoided. In addition, the application screens out the ginseng and the bletilla striata matched with the hirudin, strengthens the effect of facial mask on beautifying and skin care, and has better industrial application prospect.

Disclosure of Invention

The invention provides a cosmetic skin care mask preparation method containing hirudin, which is prepared from active ingredients and auxiliary materials, wherein the active ingredients comprise 0.2-10 parts of hirudin, 0.4-25 parts of ginseng extract and 0.4-25 parts of bletilla striata extract, and the auxiliary materials comprise 1-10 parts of xanthan gum, 40-120 parts of deionized water, 1-10 parts of sodium hyaluronate, 3-20 parts of glycerol, 2-10 parts of trehalose and 0.05-0.5 part of polyglycerol-2 oleate;

the preparation method of the mask comprises the following steps: 1) Dissolving auxiliary materials such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate and active ingredient ginseng extract and bletilla striata extract in deionized water to obtain solution 1;

2) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a swing disc of the treated semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying and pre-freezing at-30- (-80) deg.C for 2-5 hours, sublimation and resolution at 30-40 deg.C for 10-15 hours;

3) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

4) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

5) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying and pre-freezing at-30- (-80) deg.C for 2-5 hours, sublimation and resolution at 30-40 deg.C for 10-15 hours;

6) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

Further, the active ingredients of the mask comprise 0.2 part of hirudin, 0.4 part of ginseng extract and 0.4 part of bletilla striata extract, and the auxiliary materials comprise 1 part of xanthan gum, 40 parts of water, 1 part of sodium hyaluronate, 3 parts of glycerol, 2 parts of trehalose and 0.05 part of polyglycerol-2 oleate.

Further, the active ingredients of the mask comprise 0.2 part of hirudin, 0.4 part of ginseng extract and 0.4 part of bletilla striata extract, and the auxiliary materials comprise 1 part of xanthan gum, 40 parts of water, 1 part of sodium hyaluronate, 3 parts of glycerol, 2 parts of trehalose and 0.05 part of polyglycerol-2 oleate.

Further, the active ingredients of the mask comprise 10 parts of hirudin, 25 parts of ginseng extract and 25 parts of bletilla striata extract, and the auxiliary materials comprise 10 parts of xanthan gum, 120 parts of water, 10 parts of sodium hyaluronate, 20 parts of glycerol, 10 parts of trehalose and 0.5 part of polyglycerol-2 oleate.

Further, the active ingredients of the mask comprise 5 parts of hirudin, 12 parts of ginseng extract and 12 parts of bletilla striata extract, and the auxiliary materials comprise 5 parts of xanthan gum, 70 parts of water, 5 parts of sodium hyaluronate, 10 parts of glycerol, 5 parts of trehalose and 0.2 part of polyglycerol-2 oleate.

Further, the preparation method of the ginseng extract and the bletilla striata extract comprises the following steps: taking ginseng and bletilla striata, respectively superfine pulverizing, sieving, respectively adding 20 times of ethanol solution with concentration of 60% of the ginseng and the bletilla striata, ultrasonically extracting for 1.5h under the power of 480W, respectively filtering the extracting solution to obtain filtrate and filter residue, respectively adding 10 times of water of the filter residue, ultrasonically extracting for 2h under the power of 480W, respectively filtering the extracting solution to obtain filtrate and filter residue, respectively combining the 2 times of filtrate, and concentrating to 1/4 of the original volume to obtain the ginseng extract and the bletilla striata extract.

Further, the pre-freezing temperature of the freeze drying in the step 2) and the step 5) is-40- (-80) DEG C, the time is 3-5 hours, the sublimation analysis drying temperature is 35-40 ℃ and the time is 12-15 hours.

Further, the pre-freezing temperature of the freeze drying in the step 2) and the step 5) is minus 30 ℃ for 5 hours, and the sublimation analysis drying temperature is 40 ℃ for 10 hours.

Further, the pre-freezing temperature of the freeze drying in the step 2) and the step 5) is-80 ℃ for 2 hours, and the sublimation analysis drying temperature is 30 ℃ for 15 hours.

The invention provides a cosmetic skin care mask containing hirudin, which is prepared by any one of the methods.

The invention provides a using method of the beauty skin care facial mask, when in use, the facial mask is soaked or sprayed with water for 1-8 minutes, so that the xanthan gum is dissolved and then used.

The invention also relates to a preparation method of the skin-care facial mask containing hirudin, which is prepared from an active ingredient and an auxiliary ingredient, wherein the active ingredient comprises 0.2 part of hirudin, 0.8 part of ginseng extract and 0.8 part of bletilla striata extract, and the auxiliary ingredient comprises 1 part of xanthan gum, 40 parts of deionized water, 1 part of sodium hyaluronate, 3 parts of glycerol, 2 parts of trehalose and 0.05 part of polyglycerol-2 oleate;

The preparation method of the mask comprises the following steps: 1) Dissolving adjuvants such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate, half Ginseng radix extract, and half rhizoma Bletillae extract in appropriate amount of deionized water to obtain solution 1;

2) Mixing the rest Ginseng radix extract and the rest rhizoma Bletillae extract, adding the rest deionized water, bagging, sterilizing, and vacuum packaging to obtain bag 1;

3) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a tray of the semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying at-50deg.C for 3 hr, sublimation analysis at 35deg.C for 12 hr;

4) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

5) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

6) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying at-50deg.C for 4 hr, sublimation analysis at 35deg.C for 12 hr;

7) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, adding a bag 1, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

The cosmetic skin care mask containing the hirudin, which is prepared by the method, is prepared.

The use method of the facial mask comprises soaking or spraying Ginseng radix and rhizoma Bletillae extract in bag 1 for 1-8 min to dissolve xanthan gum, and then using.

Compared with the prior art, the beneficial effects that this specification reached include at least:

(1) Based on CN117297996A, the invention utilizes the characteristic that xanthan gum has better solubility in hot and cold water, the xanthan gum can be dissolved in polyalcohol glycerin in a mask, hirudin has the characteristic of thermal stability in an environment without moisture, the hirudin is uniformly dispersed in an anhydrous glycerin and xanthan gum system and then sprayed on a semi-finished product mask 1, and freeze drying is carried out for the second time to prepare the hirudin freeze-dried mask, thus solving the problems that CN117297996A firstly dissolves the hirudin in water, namely, the hirudin is placed in an environment with large moisture, the analysis is generally carried out in an environment with the temperature higher than room temperature in the freeze-drying sublimation stage, and the hirudin part is deactivated.

(2) The xanthan gum selected by the invention has better solubility in cold water and hot water, so when in use, the mask is soaked or sprayed with water, so that the hirudin loses the wrapping of the xanthan gum, and the problem that the hirudin is difficult to dissolve out when the mask is in use, thereby affecting the beautifying effect is solved.

(3) The application improves the CN117297996A technology, adopts secondary freeze drying to prepare the beauty skin care mask, namely, partial components are freeze dried firstly to prepare the semi-finished product mask 1, hirudin is uniformly dispersed in an anhydrous glycerin and xanthan gum system and then sprayed on the semi-finished product mask 1, and the anticoagulation activity of hirudin in the mask can be greatly improved by secondary freeze drying.

(4) The application screens out the ginseng and the bletilla tuber which have synergistic matching effect with hirudin from the common Chinese herbal medicines such as astragalus, angelica, ginseng, white atractylodes rhizome, angelica dahurica, bletilla striata, silkworm larva, white poria and white paeony root with the effects of beautifying and protecting skin, strengthens the effects of facial mask and has better industrial application prospect.

Drawings

FIG. 1 shows the DPPH radical scavenging effect of the combination of the different skin care and beauty herbs with hirudin in example 1.

FIG. 2 shows the effect of the combination of the different skin care and beauty traditional Chinese medicines and hirudin in example 1 on scavenging hydroxyl radicals.

FIG. 3 shows the effect of inhibiting tyrosinase activity by combining different skin care herbs with hirudin in example 1.

FIG. 4 shows the melanin synthesis inhibition effect of the combination of different skin care traditional Chinese medicines and hirudin in example 1.

Detailed Description

Specific embodiments of the present invention are described below with reference to the accompanying drawings. The experimental methods used in the implementation examples are all conventional methods unless otherwise specified; materials, reagents and the like used, unless otherwise indicated, are all commercially available.

Example 1 different cosmetic skin care traditional Chinese medicines are combined with hirudin for cosmetic skin care effect.

1) Taking common Chinese herbal medicines of astragalus membranaceus, angelica sinensis, ginseng, bighead atractylodes rhizome, angelica dahurica, bletilla striata, white muscardine silkworm, white poria cocos and white paeony root with the effects of beautifying and skin care, respectively carrying out superfine grinding and sieving, respectively adding 20 times of ethanol solution with the concentration of 60% of the traditional Chinese medicines, carrying out ultrasonic extraction for 1.5 hours at the power of 480W, respectively filtering the extracting solution to obtain filtrate and filter residues, respectively adding 10 times of water of the filter residues, carrying out ultrasonic extraction for 2 hours at the power of 480W, respectively filtering the extracting solution to obtain filtrate and filter residues, respectively merging the 2 filtrates and concentrating to 1/4 of the original volume to respectively obtain astragalus membranaceus extract, angelica sinensis extract, ginseng extract, white paeony root extract, radix angelicae dahuricae extract, bletilla striata extract, white muscardine silkworm extract and white paeony root extract for later use;

2) The hirudin is respectively added into the astragalus extract, the angelica extract, the ginseng extract, the white atractylodes rhizome extract, the angelica extract, the bletilla striata extract, the white stiff silkworm extract, the white poria extract and the white peony root extract, and the mass ratio of the extract to the hirudin is 2:1, so that the traditional Chinese medicine extract and the hirudin composition is obtained.

Referring to the measurement of DPPH free radical scavenging ability, hydroxyl free radical scavenging ability, tyrosinase activity in B16 cells and melanin content in B16 cells (concentration of the combination of traditional Chinese medicine extract and hirudin is 2.5 mg/ml) in patent CN116270287A, DPPH scavenging rate, hydroxyl free radical scavenging rate, tyrosinase activity inhibition rate and melanin synthesis inhibition rate are measured, and specific results are shown in figures 1-4.

As can be seen from fig. 1-4, after the extracts of astragalus root, angelica sinensis, ginseng, bighead atractylodes rhizome, angelica dahurica, bletilla striata, silkworm larva, white poria and white paeony root are respectively combined with hirudin, the DPPH free radical elimination rate and the hydroxyl free radical elimination rate of the ginseng group (i.e. the combination of the ginseng extract and the hirudin) and the bletilla striata group (i.e. the combination of the bletilla striata extract and the hirudin) are obviously higher than those of other groups, wherein the DPPH free radical elimination rate of the ginseng group is slightly higher than that of the bletilla striata group, and the hydroxyl free radical elimination rate of the bletilla striata group is slightly higher than that of the ginseng group;

The inhibition rate of tyrosinase activity and melanin synthesis of the ginseng group (namely the combination of the ginseng extract and the hirudin) and the bletilla striata group (namely the combination of the bletilla striata extract and the hirudin) are also obviously superior to those of other Chinese herbal medicines and the combination of the hirudin, and the inhibition rate of tyrosinase activity and melanin synthesis of the ginseng group are slightly higher than those of the bletilla striata group.

The DPPH free radical elimination rate, the hydroxyl free radical elimination rate, the tyrosinase activity inhibition rate and the melanin synthesis inhibition rate are all common measurement indexes for beautifying and protecting skin, so that the combination of the ginseng, the bletilla striata and the hirudin has good beautifying and skin-protecting effects, and therefore, the combination of the ginseng, the bletilla striata and the hirudin is selected to strengthen the beautifying and skin-protecting effects of the hirudin.

Example 2 preparation method of skin-caring facial mask containing hirudin

A preparation method of a cosmetic skin care mask containing hirudin comprises the steps of preparing a mask raw material comprising an effective component and an auxiliary material component, wherein the effective component comprises 0.2 part of hirudin, 0.4 part of ginseng extract and 0.4 part of bletilla striata extract, and the auxiliary material comprises 1 part of xanthan gum, 40 parts of deionized water, 1 part of sodium hyaluronate, 3 parts of glycerol, 2 parts of trehalose and 0.05 part of polyglycerol-2 oleate;

the preparation method of the mask comprises the following steps: 1) Dissolving auxiliary materials such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate and active ingredient ginseng extract and bletilla striata extract in deionized water to obtain solution 1;

2) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a tray of the semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying at-50deg.C for 3 hr, sublimation analysis at 35deg.C for 12 hr;

3) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

4) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

5) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying at-50deg.C for 4 hr, sublimation analysis at 35deg.C for 12 hr;

6) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

When in use, the facial mask is soaked or sprayed with water for 1-8 min to dissolve xanthan gum, and then used.

Comparative example 1: the mask was prepared using the method of CN117297996a example 1.

Comparative example 2: the same as in example 2, except that gelatin was substituted for xanthan gum.

Comparative example 3: the same as in example 2, except that xanthan gum in step 4 was omitted.

The anticoagulant activity of the masks of example 2 and comparative examples 1-3 was measured with reference to the CN117297996A example 14 stability long-term test, and is shown in Table 1 below.

As can be seen from example 2 and comparative example 1 in Table 1, in example 2, hirudin is uniformly dispersed in an anhydrous glycerin and xanthan gum system by utilizing the heat stability of hirudin in an anhydrous environment, and the hirudin is subjected to secondary freeze drying to prepare a mask, so that the anticoagulation activity of hirudin can be greatly maintained, the anticoagulation activity of hirudin is smaller than that of comparative example 1 due to the encapsulation of xanthan gum and glycerin in the early stage of example 2, the protection effect is lost due to the long-term absorption of environmental moisture by xanthan gum, and the anticoagulation activity of hirudin is rapidly reduced after 18 months.

Because gelatin is not good in cold water solubility, compared with example 2, the effect of comparative example 2 is better than that of example 2, but when the mask of comparative example 2 is used, namely the mask is soaked, the problem that hirudin is not easy to dissolve exists, so that hirudin in the mask of comparative example 2 is not easy to enter the skin surface layer, and the skin care and beauty effects are affected.

Comparative example 3, which uses only glycerin and secondary freeze drying, can better retain hirudin anticoagulation activity when prepared, but the hirudin anticoagulation activity is also reduced rapidly when left in a moist environment for a long time.

Example 3 preparation method of skin-caring facial mask containing hirudin

A preparation method of a cosmetic skin care mask containing hirudin comprises the steps of preparing a mask raw material comprising an effective component and an auxiliary material component, wherein the effective component comprises 0.2 part of hirudin, 0.8 part of ginseng extract and 0.8 part of bletilla striata extract, and the auxiliary material comprises 1 part of xanthan gum, 40 parts of deionized water, 1 part of sodium hyaluronate, 3 parts of glycerol, 2 parts of trehalose and 0.05 part of polyglycerol-2 oleate;

The preparation method of the mask comprises the following steps: 1) Dissolving adjuvants such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate, half Ginseng radix extract, and half rhizoma Bletillae extract in appropriate amount of deionized water to obtain solution 1;

2) Mixing the rest Ginseng radix extract and the rest rhizoma Bletillae extract, adding the rest deionized water, bagging, sterilizing, and vacuum packaging to obtain bag 1;

3) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a tray of the semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying at-50deg.C for 3 hr, sublimation analysis at 35deg.C for 12 hr;

4) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

5) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

6) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying at-50deg.C for 4 hr, sublimation analysis at 35deg.C for 12 hr;

7) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, adding a bag 1, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

When in use, the facial mask is soaked or sprayed with the extracts of Ginseng radix and rhizoma Bletillae in bag 1 for 1-8 min to dissolve xanthan gum, and then used.

Example 4 preparation method of skin-caring facial mask containing hirudin

A preparation method of a cosmetic skin care mask containing hirudin comprises the steps of preparing a mask raw material, wherein the mask raw material comprises an active ingredient and an auxiliary material ingredient, the active ingredient comprises 10 parts of hirudin, 25 parts of ginseng extract and 25 parts of bletilla striata extract, and the auxiliary material comprises 10 parts of xanthan gum, 120 parts of water, 10 parts of sodium hyaluronate, 20 parts of glycerol, 10 parts of trehalose and 0.5 part of polyglycerol-2 oleate;

the preparation method of the mask comprises the following steps: 1) Dissolving auxiliary materials such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate and active ingredient ginseng extract and bletilla striata extract in deionized water to obtain solution 1;

2) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a tray of the semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying at-30deg.C for 5 hr, sublimation analysis at 40deg.C for 10 hr;

3) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

4) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

5) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying at-30deg.C for 5 hr, sublimation analysis at 40deg.C for 10 hr;

6) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

Example 5 preparation method of skin-caring facial mask containing hirudin

A preparation method of a cosmetic skin care mask containing hirudin comprises the steps of preparing a mask raw material comprising an effective component and an auxiliary material component, wherein the effective component comprises 5 parts of hirudin, 12 parts of ginseng extract and 12 parts of bletilla striata extract, and the auxiliary material comprises 5 parts of xanthan gum, 70 parts of water, 5 parts of sodium hyaluronate, 10 parts of glycerol, 5 parts of trehalose and 0.2 part of polyglycerol-2 oleate;

the preparation method of the mask comprises the following steps: 1) Dissolving auxiliary materials such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate and active ingredient ginseng extract and bletilla striata extract in deionized water to obtain solution 1;

2) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a tray of the semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying at-80deg.C for 2 hr, sublimation analysis at 30deg.C for 15 hr;

3) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

4) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

5) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying at-80deg.C for 2 hr, sublimation analysis at 30deg.C for 15 hr;

6) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

While the invention has been described with reference to the preferred embodiments, it is not limited thereto, and various changes and modifications can be made therein by those skilled in the art without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (10)

1. A preparation method of a cosmetic skin care mask containing hirudin is characterized by comprising the following steps: the mask is prepared from active ingredients and auxiliary materials, wherein the active ingredients comprise 0.2-10 parts of hirudin, 0.4-25 parts of ginseng extract and 0.4-25 parts of bletilla striata extract, and the auxiliary materials comprise 1-10 parts of xanthan gum, 40-120 parts of deionized water, 1-10 parts of sodium hyaluronate, 3-20 parts of glycerol, 2-10 parts of trehalose and 0.05-0.5 part of polyglycerol-2 oleate;

the preparation method of the mask comprises the following steps: 1) Dissolving auxiliary materials such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate and active ingredient ginseng extract and bletilla striata extract in deionized water to obtain solution 1;

2) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a tray of the semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying and pre-freezing at-30- (-80) deg.C for 2-5 hours, sublimation and resolution at 30-40 deg.C for 10-15 hours;

3) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

4) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

5) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying and pre-freezing at-30- (-80) deg.C for 2-5 hours, sublimation and resolution at 30-40 deg.C for 10-15 hours;

6) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

2. The method for preparing the skin care facial mask containing the water-hirudin according to claim 1, wherein the effective components comprise 0.2 part of hirudin, 0.4 part of ginseng extract and 0.4 part of bletilla striata extract, and the auxiliary materials comprise 1 part of xanthan gum, 40 parts of water, 1 part of sodium hyaluronate, 3 parts of glycerol, 2 parts of trehalose and 0.05 part of polyglycerol-2 oleate.

3. The method for preparing the skin care facial mask containing the hirudin according to claim 1, wherein the effective components comprise 10 parts of hirudin, 25 parts of ginseng extract and 25 parts of bletilla striata extract, and the auxiliary materials comprise 10 parts of xanthan gum, 120 parts of water, 10 parts of sodium hyaluronate, 20 parts of glycerol, 10 parts of trehalose and 0.5 part of polyglycerol-2 oleate.

4. The method for preparing the skin care facial mask containing the hirudin according to claim 1, wherein the effective components comprise 5 parts of hirudin, 12 parts of ginseng extract and 12 parts of bletilla striata extract, and the auxiliary materials comprise 5 parts of xanthan gum, 70 parts of water, 5 parts of sodium hyaluronate, 10 parts of glycerol, 5 parts of trehalose and 0.2 part of polyglycerol-2 oleate.

5. The method for preparing the skin care facial mask containing the hirudin according to claim 1, wherein the preparation method of the ginseng extract and the bletilla striata extract is as follows: taking ginseng and bletilla striata, respectively superfine pulverizing, sieving, respectively adding 20 times of ethanol solution with concentration of 60% of the ginseng and the bletilla striata, ultrasonically extracting for 1.5h under the power of 480W, respectively filtering the extracting solution to obtain filtrate and filter residue, respectively adding 10 times of water of the filter residue, ultrasonically extracting for 2h under the power of 480W, respectively filtering the extracting solution to obtain filtrate and filter residue, respectively combining the 2 times of filtrate, and concentrating to 1/4 of the original volume to obtain the ginseng extract and the bletilla striata extract.

6. The method for preparing the skin care facial mask containing the hirudin according to claim 1, wherein the pre-freezing temperature of the freeze drying in the step 2) and the step 5) is-30 ℃ for 5 hours, and the sublimation-analysis drying temperature is 40 ℃ for 10 hours.

7. The method for preparing the skin care facial mask containing the hirudin according to claim 1, wherein the pre-freezing temperature of the freeze drying in the step 2) and the step 5) is-80 ℃ for 2 hours, and the sublimation-analysis drying temperature is 30 ℃ for 15 hours.

8. The cosmetic skin care mask comprising hirudin prepared by the process of any one of claims 1 to 7.

9. A preparation method of a cosmetic skin care mask containing hirudin is characterized by comprising the following steps: the mask is prepared from active ingredients and auxiliary materials, wherein the active ingredients comprise 0.2 part of hirudin, 0.8 part of ginseng extract and 0.8 part of bletilla striata extract, and the auxiliary materials comprise 1 part of xanthan gum, 40 parts of deionized water, 1 part of sodium hyaluronate, 3 parts of glycerol, 2 parts of trehalose and 0.05 part of polyglycerol-2 oleate;

The preparation method of the mask comprises the following steps: 1) Dissolving adjuvants such as sodium hyaluronate, half glycerol, trehalose, polyglycerol-2 oleate, half Ginseng radix extract, and half rhizoma Bletillae extract in appropriate amount of deionized water to obtain solution 1;

2) Mixing the rest Ginseng radix extract and the rest rhizoma Bletillae extract, adding the rest deionized water, bagging, sterilizing, and vacuum packaging to obtain bag 1;

3) Sterilizing the mask cloth, folding, immersing in the solution 1, standing, fully absorbing the solution 1 by the mask cloth to obtain a semi-finished product 1, pushing a tray of the semi-finished product 1 into a freeze dryer, and performing first freeze drying to obtain a first freeze-dried mask; freeze drying at-50deg.C for 3 hr, sublimation analysis at 35deg.C for 12 hr;

4) Heating the rest half of glycerol to 65deg.C, adding xanthan gum, and stirring to dissolve to obtain xanthan gum glycerol composition;

5) Adding hirudin into the xanthan gum glycerol composition, stirring uniformly, and rapidly spraying onto the surface of a once freeze-dried mask to obtain a semi-finished product 2;

6) Pushing the semi-finished product 2 swing disc into a freeze dryer, and performing secondary freeze drying to obtain a secondary freeze-dried mask; freeze drying at-50deg.C for 4 hr, sublimation analysis at 35deg.C for 12 hr;

7) Rapidly packaging the freeze-dried semi-finished product 2 into a facial mask packaging bag, adding a bag 1, performing cobalt radiation sterilization treatment on the facial mask packaging bag, vacuumizing the facial mask packaging bag in a nitrogen environment, and sealing to obtain the cosmetic skin care facial mask containing hirudin.

10. The cosmetic skin care mask comprising hirudin prepared by the method of claim 9.