CN1179646C

CN1179646C - Aggregation-preventing nanometer wide-spectrum antibacterial silve powder and its inductrial production process

Info

Publication number: CN1179646C
Application number: CNB011107677A
Authority: CN
Inventors: 朱红军
Original assignee: Individual
Current assignee: SHENZHEN YUANXING NANO-PHARMACEUTICAL Co Ltd
Priority date: 2001-04-20
Filing date: 2001-04-20
Publication date: 2004-12-15
Anticipated expiration: 2021-04-20
Also published as: CN1328827A; CN1322474A; CN1505551A; CN1224479C; CN2479952Y; CN1328819A; WO2002090025A1; CN1169529C

Abstract

The present invention discloses aggregation preventing broad-spectrum antibacterial nanometer silver macro-powder and an industrial production manufacture process. The broad-spectrum antibacterial nanometer silver micro-powder comprises superfine silver particles firmly attached among fiber elements and on fibers of natural porous vegetable materials, wherein the particle diameters of the superfine silver particles are from 1 to 100 nanometers, the surfaces of the superfine silver particles are coated with silver oxide of 2 to 8 nanometers, and the cores of the superfine silver particles are element silver. A method of the present invention can prevent the superfine silver particles with strong activity from aggregating. The nanometer silver micro-powder has strong antibacterial capability and broad-spectrum antibiosis; when in contact with water, the nanometer silver micro-powder has stronger bactericidal power and has no toxicity, no stimulation, no pathergy and no drug resistance. Moreover, the nanometer silver micro-powder can be used as raw medicinal materials.

Description

Aggregation-preventing nanometer wide-spectrum antibacterial silve powder and inductrial production process

The invention relates to a kind of wide-spectrum antiseptic nanometer silver material and inductrial production process, more particularly, is about a kind of aggregation-preventing nanometer wide-spectrum antibacterial silve powder and inductrial production process.

Known from institute, nanometer technology is that it is one of three big technology of 21 century economic development with the material of 1～100 nanometer particle size new subject as development and research object, and it also is the most possible new technology of the breakthrough achievement of generation.Huge fund is competitively thrown in countries in the world, the collection elite does all one can to develop nanometer technology and application thereof, application and the industrialization that drops into exploitation upsurge, the especially nanometer technology of nanometer technology down to colleges and universities, the numerous and confused great dynamics of scientific research institution of basic unit extremely at central level will be the most important thing of China's research and development in China.

The antibiotic example of many relevant silver is disclosed in the prior art, as in the clear 54-151669 of Japan Patent, a kind of bactericidal properties cloth is disclosed, its compound resin solution independent or mixture that will contain copper, silver (6 microns of average grain diameters) is handled yarn, be made into bactericidal properties cloth again after making this solution evenly be coated on the surface of yarn, this product can be made the lining of rubber boot, canvas shoe and socks.

In Japan " process technology " vol.17 NO.7 report, handle acrylic fiber with copper and sulphide, get acrylic fibers-copper sulfide compound, it has bacteriostasis to staphylococcus aureus, Escherichia coli, hay bacillus, the thread tinea alba bacterium of skin.

At Chinese invention patent CN-87100231A, exercise question is " antibiotic odourproof fiber yarn and a manufacture method ", open day: disclose a kind of antimicrobial yarn in 18 days November in 1987, it is with acrylic fibers yarn priority and copper, Viride Nitens-4 composite crosslinking, product has bacteria resistance function to 10 bacterial classifications such as staphylococcus aureus, MRSA, white Portugal coccus, Candida albicanss, and it can be used as underwear, socks, shoe-pad and the medical industry of antibacterial deodourizing, the work clothes of food industry.

At the flat 3-136649 of Japan Patent, the applying date " discloses a kind of anti-bacteria cloth that prevents mastitis for milk cows in 24 days October in 1989.It answers platform with silver ion and polyacrylonitrile with the coordinate bond form, and product has inhibitory action to 6 kinds of bacterial classifications such as streptococcus, staphylococcus, can be used as the antimicrobial wipe of wiping cow breast nipple with the mastitis of prevention milk cow.

But above-mentioned document or report all do not relate to Nano Silver and suitable carrier as anti-biotic material.

More than these products antibacterial action is all arranged, but antimicrobial spectrum is wide, effect is not obvious, and different fully with the present invention.

One of purpose of the present invention provides a kind of wide-spectrum antiseptic nanometer silver micro mist and inductrial production process.

Two of purpose of the present invention provides a kind of technology of gathering between the active extremely strong silver ultramicron of preventing.

Three of purpose of the present invention provides a kind of inductrial production process of aggregation-preventing nanometer wide-spectrum antibacterial silve powder.

These and other objects of the present invention will and be described and further set forth by following detailed description.

In the present invention, aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention is being attached with ultra micron silver between the fiber element method of natural porous crack vegetable material with on the fiber securely, and its particle diameter is 1～100 nanometer, the top layer of this ultra micron silver is the silver oxide of 2～8 nanometers, and core is an elemental silver; Between the fiber element method of natural porous crack vegetable material with fiber on the silver content of the Nano Silver that adheres to be 0.1～200 milligram/gram; Described natural porous crack vegetable material is the fibre stuff of rush, cotton, hair, fiber crops, silk, bamboo and wood, and stem, activated carbon and the chemical fibre of reed catkins, luffa plant, non-woven fabrics fabric, foamed plastics etc.

The inductrial production process of aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention comprises the vacuum pre-treatment of carrier material, the preparation of medicament, dipping, supercharging homogeneous, Chemical Physics reaction, cleaning, drying and pulverising step.

Further, in the inductrial production process of aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention, described vacuum pre-treatment comprises medicinal alcohol (75～98%) with 1～20% (V/V) with natural porous crack vegetable material (particularly rush material) submergence, and vacuum extraction must not have the natural porous of the solid crack vegetable material of organic principle then.

In the inductrial production process of aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention, described medicament preparation formula is (by a carrier after 10 kilograms of extractions):

A, silver nitrate 0.01～1M, ammoniacal liquor 0.05～0.5M, sodium hydroxide 0.01～0.3M

Adding water to cumulative volume is 500 liters;

B, glucose or ascorbic acid 1～8M, nitric acid 0.02～0.2M, ethanol 2～10M,

Adding water to cumulative volume is 500 liters;

10 parts by volume A are added that 1 parts by volume B mixes the back and placed 10～40 minutes,, carry out the even matter of supercharging then, make it evenly to put into reactor and add dispersant and react, be pale brown look to product the medicament impregnated carrier.

In aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention, prepared nano silver powder is added suitable medicinal adjuvant or excipients can make pulvis, tablet, paste, creme, film, gel, patch, injection, aqua, spray and biological medicine chip; Prepared silver powder can be used as the sterilization material of various uses in field, battlefield, disaster area, the space industry, is used for killing the pathogenic bacteria of infected water.

In the present invention, if necessary, can also add the processing method of some industry routines, can certainly comprise in conjunction with the conventional knowledge of this specialty and omit one or more steps by actual conditions, as long as but can prepare product.

Product of the present invention can be used as medical (outward) material of treatment burn and scald, material with the skin infection for the treatment of traumatic patient and superficial skin fungus infection and prevention of surgical incision postoperative and treatment infection of incisional wound, also can be used as daily living article, has broad spectrum antibacterial, long-lasting, kill the drug resistance pathogenic bacteria, hydrophily-chance water sterilizing power is stronger, avirulence, nonirritant, no hypersensitivity, excellent properties such as have no drug resistance.Can be used as skin infection and the dermatophytid infection and the surgical incision infection for the treatment of traumatic patient.Scene control trauma infection contamination under the unsuitable environmental conditions such as the war wound of especially field work, war wound, the seawater pollution surface of a wound, flood, fire, earthquake.The more important thing is that product of the present invention can be used for the treatment of the intractable bedsore and infect, empyrosis wound surface infects, diabetes later stage lower limb telephium, women's bacillary, mycotic and the colpitic control of mixed infection, difficult sexuality such as male prostatitis is dyed, and has started the industrialization example that nanometer technology is used at the human body anti infection region.

Technology of the present invention can prevent to gather effect between the silver ultramicron of the nanoscale particle diameter that newly-generated surface-active is very strong, thereby prevent to generate the bigger silver particle of particle diameter in manufacturing process.Through making in laboratory, pilot scale, and go into operation successfully, production run and constant product quality, annual value of production are estimated can reach more than 1,000,000,000 yuan.

To the present invention further be described by accompanying drawing and flow chart below.

Fig. 1 does not adopt the silver sample of this technology at microscopical observation photo.

Fig. 2 does not adopt the silver sample of this technology at microscopical observation photo.

Fig. 3 does not adopt the silver sample of this technology at microscopical observation photo.

Fig. 4 adopts the silver sample of the technology of the present invention at microscopical observation photo

Fig. 5 is a process chart of the present invention.

Aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention (being designated hereinafter simply as PNSM) belongs to the organic-inorganic nano composite material of having disperseed inorganic nano-particle in the nanomaterial science on natural organic substrate.The carrier of PNSM of the present invention is selected for use from numerous materials that can be used as carrier that we grasp, and takes the carrier of rush as PNSM..Organic carrier is a rush, and inorganic nano is the ultra micron silver of 1～100 nanometer for particle diameter then, can be used as functional material and specifically is applied in the human body anti infection region.About defining of nano material, except particle diameter was 1～100 nanometer, also must possess had this kind nano material to compare with the homogeneous material of macro-size, and the former has the specific performance that is much better than the latter on some important performance.Promptly will have particle diameter 1～100 nanometer and specific performance simultaneously, both are indispensable, otherwise just can not be defined as nano material.The nanometer ultramicron that with particle diameter is 1～100 nanometer is compared with macroscopical particulate of homogeneity, and the former specific surface is very big, and is inversely proportional to particle size.For example: it is 5 nanometers that 1 gram copper is worked as particle diameter, and its specific surface is 130 meters ²/ gram; Particle diameter is 2 nanometers, and its specific surface is 330 meters ²/ gram; Add skin effect simultaneously and promptly be distributed in the percentage by volume of particle surface, when particle diameter is 5 nanometers, its surface volume percentage is 50%, then be 80% when particle diameter is 2 nanometers, cause former coordination deficiency because reducing of particle diameter increases sharply the atomicity that is in superficial layer this moment, unsaturated bond exposes and increases, the surface of atom can be increased, and causes the chemism of these nano level ultramicrons extremely strong, thereby works as the moment that these ultramicrons have just generated, inevitably mutual collision will take place, then these two of bumping, even a plurality of ultra micron will gather effect, and it is bigger to cause generating particle diameter thus, can be greater than former particle diameter N, No, Noo macroparticle doubly, even owing to gathering the particle that effect becomes macro-size, corresponding its chemism just reduces greatly, loses the character of nano material the most at last.This shows that the present invention adopts suitable chemistry and physical means to reach to prevent the effect of gathering between newly-generated ultramicron.This anti-centralizing technique is for based on chemical method, and the physics method is that the method success or not of making 1～100 nanometer ultramicron of assisting is vital.In order to prove conclusively the effect of anti-centralizing technique, we carry out the silver sample that adopts and do not adopt this technology, and electron microscope detects and photograph, and its effect is significantly, please referring to Fig. 1, Fig. 2, Fig. 3 and Fig. 4.

In Fig. 1～3 owing to do not adopt the product of anti-centralizing technique of the present invention, the deposit of spheroidal particle in the image, its particle diameter is much larger than the particle diameter of spheroidal particle.

Fig. 4 is for adopting the electron microscopic image of the PNSM that prevents centralizing technique, the particle diameter that clearly demonstrates the ultramicron of the last exhausted major part of PNSM is 5～40 nanometers, and it is neat, even to distribute, there is strip protuberance at the photo middle part, but can find out clearly that it is not that spherical superfine particle is piled up, may be the tump of cilium surface in the carrier according to one's analysis.In addition in order to measure the composition that PNSM goes up ultramicron, measuring these its top layers of spherical ultramicron through photoelectron spectrograph is silver oxides of 2～8 nanometer thickness, its core is an elemental silver, and with American Pharmacopeia and British Pharmacopoeia institute periodical method, the silver amount that mensuration PNSM goes up Nano Silver is 0.1～200 a milligram/gram.

The function of PNSM of the present invention and detection test thereof are as follows:

PNSM of the present invention measures through electron microscope and photoelectron spectrograph, and as seen the exhausted most particle diameter of its silver particle is the ultramicron of 5～40 nanometers, consists of Ag ₂O+Ag, the top layer of particle is Ag ₂O, its core is an elemental silver so can be defined as the silver particle of nanoscale particle diameter clearly, as for whether excellent property being arranged, will confirm by following every detection, testing result sees Table 1 and table 2.

Table 1 is to be detected by medicine test center Microbiological Lab of the entire PLA of the Chinese People's Liberation Army.

Table 2 is to be detected by Huashan hospital of Shanghai Medical Univ Bacteriology Room.

The antibiotic mechanism of PNSM of the present invention is classical antibiotic mechanism:

Therefore PNSM is by the most classical antibiotic mechanism, in the enzyme of depending on for existence in the thalline-SH group displacement one-tenth-SAg makes the enzyme loss of activity cause germ death, and its product is enzyme 2SAg and H ₂There is not the secondary paathogenic factor in O.

By table 1, the PNSM of the present invention as can be seen of the experimental result shown in the table 2 has following superior specific function.

1, antibiotic broad spectrum activity:

Bacterial classification shown in the table 1, table 2 has comprised grampostive bacteria, gram-negative bacteria, fungi, brood cell and anaerobic bacteria, and antimicrobial spectrum is very rarely seen in existing antibacterials so widely.With regard to table 1 and the related bacterial classification of table 2 remove repetition in respect of 38 bacterial classifications, and most bacterial classification is to separate from the strong clinical patients juice of resistance.

2, long-lasting:

Washing shown in the table 1, table 2 is for 50 times and 20 times that PNSM washes the clear water rinsing by rubbing with the hands 50 times and 20 times with soap is manual at every turn.Wash is for 100 times to wash meter by rubbing with the hands 100 times with clear water at every turn, and through the PNSM. of washing, its bacteriostasis property does not have obviously and goes down.

3, PNSM and 8 antimicrobials are to the comparison of pathogenic bacteria fungistatic effect:

Table 1 is depicted as PNSM. of the present invention (28) and erythromycin (7), and ampicillin (4), Zinacef (4), cefotaxime (15), OXA (2), gentamicin (6), (14) 8 antimicrobials of Ciprofloxacin carry out extracorporeal bacteria inhibitor test simultaneously.Numeral in the bracket of each nomenclature of drug back is the antibacterial strain number of this medicine energy.The anti-drug resistance pathogenic bacteria that this shows PNSM can want much superior by these 8 antimicrobials of force rate.This is for the growth of breaking current drug-resistance of bacteria, and the difficult situation that has made antibiotic exploitation be unable to catch up with the bacterial drug resistance growth can be placed hopes on.By on can predict PNSM of the present invention and may progressively become and substitute antibiotic alternative medicine.

4, hydrophily-chance water sterilizing power is stronger

From table 1, the visible PNSM of table 2 is through repeatedly washing by rubbing with the hands, and its antibacterial power does not have obviously and goes down, and its dominant mechanism is exactly the Ag on the PNSM ₂O tool sustained release performance, generally under the condition that has water to exist, even the water yield of the moisture state the when humidity of body surface or application enough makes Ag ⁺Disengage, as [Ag ⁺] reach 0.n-5ppm[10 ^-6] time can reach Ag ⁺Dynamic equilibrium, and Ag ⁺Minimum inhibitory concentration be 2 * 10 ^-11M, can not produce as for the antibiotic mechanism of PNSM. with in antibiotic process the secondary paathogenic factor in front the part of " antibiotic mechanism " illustrate.Also carried out following test in addition, got the meal of 0.5 gram PNSM, soak with 500 ml waters every day, changes water every day once, and the antibacterial power of this PNSM meal does not have obviously and goes down after 45 days.So with PNSM is the externally applied drug that bulk drug is made, chance water antimicrbial power is stronger.This scalds wet treatment to current burning is very favourable.Its curative effect of clinical verification of hospital general, Chinese People's Liberation Army Nanjing is fine, The Fourth Military Medical University of P.L.A and attached Xijing hospital thereof, in-situ curing to the war wound of seawater pollution, the curative effect that confirms PNSM of the present invention is fine, the real genus top standard of the world today is so be specially adapted to do under the unsuitable environmental conditions such as field work and battlefield the infection of control wound.

5, pharmacokinetic

Through new drug Research on Safety Assessment center, national Beijing and hospital general's pharmacology section of Nanjing Military Command, be subjects with mouse, cavy and white big ear rabbit.With PNSM is that experimental drug is through the skin acute toxicity experience, irritation test, sensitivity test and chmice acute toxicity test, maximum tolerance determination test, and cumulative toxicity test, especially mouse (9) single is irritated stomach and is given the PNSM amount and be 925 milligrams/kilogram of maximal tolerance doses, be equivalent to the people and intend with clinical dosage 4625 times, observed 14 days continuously after the administration, do not find poisoning symptom and death, and growing state is better.Confirm thus, the PNSM avirulence, nonirritant, no hypersensitivity has no drug resistance.

6, the external antibacterial contrast of the silver-colored particulate of PNSM and homogeneity macroscopic view particle diameter

Directly be 6 millimeters ringlet with the garden, placing PNSM and particle diameter respectively is micron-sized silver micro mist, and the former silver content only is the latter's 10 ^-3-10 ^-1Yet on external antibacterial agar MH flat board, the former antibacterial ring is 12～20 millimeters, and the latter does not have antibacterial ring.This shows that on bacteriostasis the homogeneity silver particulate and the PNSM of macro-size are incomparable.

In sum, the function of PNSM of the present invention comprises antibiotic broad spectrum activity, long-lasting, to having the bacteriostasis of drug resistance pathogenic bacteria, hydrophily-chance water sterilizing power is stronger, avirulence (comprising no cumulative toxicity), nonirritant, no hypersensitivity, aspect such as have no drug resistance all have and are much better than current antimicrobial commonly used, also be much better than the homogeneity micro silver powder of macro-size, thus the silver particle on the PNSM of the present invention not only met particle diameter be 1～100 nanometer simultaneously but also show special superior anti-microbial property.So the silver particle on the PNSM of the present invention can be defined as Nano Silver without doubt.

The essence of PNSM is exactly to have adopted anti-centralizing technique of the present invention and produce at natural light weight, evenly and has securely adhered to the silver (Ag of the ultramicron of particle diameter 1～100 nanometer on mushy rush ₂O+Ag) particle.Final powder is broken into the micron order micro mist, promptly gets PNSM.

Manufacture craft of the present invention is: choose support material and add ingredients 1 carry out vacuum extraction, dipping in the vacuum pre-treatment, when flooding, add ingredients 2 and ingredients 3 supercharging homogeneous, carry out the Chemical Physics reaction again, in Chemical Physics reaction, add public powder, clean then, drying, pulverizing, smart powder be finished product.

In the present invention, all raw materials and assistant agent and additive all can be buied from market, dispersant is that general anti-of the industry gathers agent, concrete kind can check in from handbook, for example, available is isopropyl alcohol, neopelex, succinate sodium sulfonate, colloid palladium or colloid palladium, OP-10, trialkyl phosphates class such as tributyl phosphate, trioctyl phosphate, tri-isoamyl phosphate, the different monooctyl ester of tri o cresyl thiophosphate etc., also can circulate a notice of the 49th～57 page, Shen Junquan " the fine ceramics preparation of a micro mist " literary composition with reference to 1986 the 2nd phase silicate.

Below further specify the present invention by specific embodiment, but embodiment only be used for the explanation can not limit the scope of the invention.

If not refer in particular to all parts, measure the unit of weight that is based on gross weight in the present invention, " M " is molar concentration.

Example 1

By following amount preparating liquid (by 10 kilograms of carriers)

A、AgNO ₃ 0.5M， NH ₃·H ₂O 0.3M， NaOH 0.1M，

Adding water to cumulative volume is 500 liters;

B, glucose 4M, HNO ₃0.1M, to boil 1～5 minute, the cooling back adds ethanol makes into 10M, and volume is 50 liters.

Carrier (rush) is cleaned, added the medicinal alcohol and the distilled water (the two ratio is 1: 15) of 98% concentration, with the carrier submergence, vacuum extraction to carrier does not have organic principle and impurity, and extract is for using.

Can put into 10 kilograms of carriers of handling well (rush) for dipping usefulness with placing behind 10 parts+1 part B (V/V) mixing after 10～40 minutes.Dipping back supercharging homogeneous mixes soup and carrier, enter reactor and carry out also adding continuously in chemistry and the physical treatment dispersant-OP-10 (under ventilation condition and under the agitation as appropriate condition), be pale brown look to rush, then through clean, dry, pulverize and promptly get aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention.

Example 2

By following amount preparating liquid (by 10 kilograms of carriers)

A、AgNO ₃ 0.3M， NH ₃·H ₂O 0.2M， NaOH 0.07M，

Adding water to cumulative volume is 500 liters;

B, ascorbic acid 3M, HNO ₃0.1M, to boil 1～5 minute, the cooling back adds ethanol makes into 10M, and volume is 50 liters.

Carrier (rush) is cleaned, added the medicinal alcohol and the distilled water (the two ratio is 1: 10) of 75% concentration, with the carrier submergence, vacuum extraction to carrier does not have organic principle and impurity, and extract is for using.

Can put into 10 kilograms of carriers of handling well (rush) for dipping usefulness with placing behind 10 parts+1 part B (V/V) mixing after 10～40 minutes.Dipping back supercharging homogeneous mixes soup and carrier, enter reactor and carry out chemistry and physical treatment, add neopelex as dispersant (under ventilation and agitation as appropriate condition), be pale brown look to rush, then through clean, dry, pulverize and promptly get aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention.

Aggregation-preventing nanometer wide-spectrum antibacterial silve powder of the present invention is based on the exploitation medical product, and the antimicrobial that can be for external application after clinical verification develops to antimicrobial for oral administration, substitutes antibiotic partially.Other a plurality of industries of meter can also be related to simultaneously, bedding, footgear and clothes can be made as textile industry; Industry can be made antimicrobial form washing agent, antimicrobial form shampoo, toothpaste type, toiletries and treatment acne facial mask on dailyization; Can make to print paper money paper, paper tableware, books, file and paper in the paper-making industry industry; Can be used as the sterilization of fruit, vegetables, anticorrosion and antistaling agent in food service industry; Can be used as mould proof antibacterial paint etc. in building materials industry; Can be used as aqueous disinfectant, antimicrobial form towel and food and restaurant service industry disinfectant in tourist industry; Can be used as anti-biotic material in antimicrobial form sanltary towel, antimicrobial form baby diaper and air-conditioner, the air freshener at healthcare industry.

Table 1

The antibacterial result of PNSM and 8 kinds of antibacterials

PNSM and control group bacteriostatic diameter (mm) Antibacterials MIC method drug sensitivity tests

Celebrate in the big ring in the bacterium name source not negative erythromycin benzene azoles ammonia benzyl cephalo cephalo cephalo of washing washing washing

The 50 times 100 times husky stars of contrast XiLin, XiLin his pyridine mycin of azoles quinoline furan oxime

Staphylococcus aureus ATCC25923 18 15 15 7 24 S S S S S S S

Escherichia coli ATCC25922 13 13 12 7 10 S S S S S S

Pseudomonas aeruginosa ATCC27853 12 12 12 76 S S S

C.perfringens CMCC (B) 64,606 10

Staphylococcus aureus (MRSA) secretion 17 15 15 76 R R R R R R R

Staphylococcus epidermidis (MRSE) secretion 18 15 16 76 R R R R R R R

Micrococcus scarlatinae secretion 98876 S S S S S S S

Diplococcus gonorrhoeae secretion 10 10 10 7 31 S S S S S S

Escherichia coli secretion 17 14 14 76 R R R R R R

Enterobacter cloacae secretion 9887 11 R R R R R R

Clostridium perfringen secretion 14 12 13 76 R R R R R R

Pseudomonas aeruginosa secretion 15 15 15 76 R R R R R R

Stenotrophomonas maltophilia secretion 14 13 14 76 R R R S R S

Acinetobacter bauamnnii secretion 13 12 12 7 19 R R R S S S

Klebsiella Pneumoniae secretion 15 14 14 76 R R R S R S

Serratia marcesens secretion 16 13 14 76 R R R R R S

Take labor ground citrobacter secretion 11 10 11 76 R R R R R R

Thunder amasss Pu Luoweideng bacterium secretion 15 13 13 76 R R R S R S

Aeromonas hydrophila secretion 13 11 13 7 16 R R R S R S

Aeromonas sobria secretion 14 12 12 7 17 R I I S R S

Vibrio vulnificus secretion 17 15 15 76 R R R S I S

Proteus mirabilis secretion 11 10 10 79 R R R S R R

Proteus vulgaris secretion 11 9 11 76 R R R S R R

Pan Shi proteus secretion 10 9 10 76 R R I S R S

Candida albicans secretion 21 20 20 76

Candida tropicalis secretion 18 17 18 76

Candida parapsilosis secretion 20 19 20 76

Torulopsis glabrata secretion 27 26 26 76

Annotate: S: responsive I: the R of intermediary: resistance

Table 2

The blank AB fabric of bacterial strain PNSM

Fabric is washed 20 sub-high pressures and is not washed height and compress and wash and wash for 20 times 50 times

Pseudomonas aeruginosa----15 13 16 16

Staphylococcus aureus----15 17 15 15

Escherichia coli----13 14 18 15

MRSA - - - - 14 14 17 15

Wax-shaped bacillus---11 13 13 15 15

Vibrio vulnificus----16 16 16 17

Candida albicans-21--11 11 11 11

B group hemolytic streptococcus----11 11 12 11

Pseudomonas Maltophilia----12 12 15 14

Nitrate-negative bacillus----14 14 13 15

Moscow' paratyphi C-12--12 14 19 14

Arizona bacterium----14 14 15 14

Citrobacter----14 14 15 13

Pneumobacillus----13 13 17 13

Hay bacillus----12 12 12 12

Morganella morganii---10 12 12 12 12

Alcaligenes faecalis----14 12 16 16

Si Shi Pu Tufeideng Salmonella----12 12 14 13

Enterobacter cloacae----13 13 13 13

Gonococcus----11 11 11 11

Above result shows that PNSM all has inhibitory action to 20 strain bacteriums, and the bacterium that commute develops immunity to drugs, as staphylococcus aureus persister (MRSA), Pseudomonas aeruginosa, Pseudomonas Maltophilia and nitrate-negative bacillus etc. all have inhibitory action, high pressure and wash 20 times and 50 times after all with without the washing the same, bacteriostasis there is not obvious influence, and the AB fabric is after washing 20 times, all can not suppress above bacterium, this shows that the scope of restraining fungi of PNSM extensively and not can influence fungistatic effect because of washing than AB fabric.

Claims

1, a kind of aggregation-preventing nanometer wide-spectrum antibacterial silve powder, it is characterized in that being attached with ultra micron silver securely between the fiber element method of rush with on the fiber, its particle diameter is 5～40 nanometers, the top layer of this ultra micron silver is the silver oxide of 2～8 nanometers, core is an elemental silver, between the fiber element method of rush with fiber on the silver content of the Nano Silver that adheres to be 0.1～200 milligram/gram.

2, the manufacture craft of aggregation-preventing nanometer wide-spectrum antibacterial silve powder as claimed in claim 1 is characterized in that comprising the vacuum pre-treatment of carrier material, the preparation of medicament, dipping, supercharging homogeneous, Chemical Physics reaction, cleaning, drying and pulverising step.

3, the manufacture craft of aggregation-preventing nanometer wide-spectrum antibacterial silve powder as claimed in claim 2, it is characterized in that described vacuum pre-treatment comprises medicinal alcohol with 1～20% (V/V) with the rush submergence, vacuum extraction must not have the solid rush material of organic principle then.

4, the manufacture craft of aggregation-preventing nanometer wide-spectrum antibacterial silve powder as claimed in claim 2 is characterized in that carrier is counted after described medicament preparation formula is by 10 kilograms of extractions:

A, silver nitrate 0.01～1M, ammoniacal liquor 0.05～0.5M, sodium hydroxide 0.01～0.3M, adding water to cumulative volume is 500 liters;

B, glucose or ascorbic acid 1～8M, nitric acid 0.02～0.2M, ethanol 2～10M, adding water to cumulative volume is 500 liters;

10 parts by volume A are added that 1 parts by volume B mixes the back and placed 10～40 minutes.

5, the manufacture craft of aggregation-preventing nanometer wide-spectrum antibacterial silve powder as claimed in claim 2 is characterized in that the medicament impregnated carrier, carries out the even matter of supercharging then, makes it evenly to put into reactor and react, and adds dispersant, is pale brown look to product.

6, the manufacture craft of aggregation-preventing nanometer wide-spectrum antibacterial silve powder as claimed in claim 2 is characterized in that prepared nano silver powder is added medicinal adjuvant or excipients can make pulvis, tablet, paste, creme, film, gel, patch, injection, aqua, spray and biological medicine chip.

7,, it is characterized in that prepared silver powder can be used as the sterilization material of various uses in field, battlefield, disaster area, the space industry, is used for killing the pathogenic bacteria of infected water as the application of the prepared aggregation-preventing nanometer wide-spectrum antibacterial silve powder of claim 2.