CN117918458A

CN117918458A - Nutritional supplement for promoting recovery of wounds of pets and preparation method of nutritional supplement

Info

Publication number: CN117918458A
Application number: CN202410114039.XA
Authority: CN
Inventors: 崔心江; 吕翠; 杨智国; 孙旭东
Original assignee: Shandong Yahua Biological Technology Co ltd
Current assignee: Shandong Yahua Biological Technology Co ltd
Priority date: 2024-01-27
Filing date: 2024-01-27
Publication date: 2024-04-26

Abstract

The invention provides a nutritional supplement for promoting wound healing of pets and a preparation method thereof, belonging to the field of nutritional supplements. The preparation method of the nutritional supplement for promoting the recovery of wounds of pets comprises the following steps: preparing a first active ingredient, preparing a second active ingredient, compounding, mixing. The nutritional supplement for promoting the recovery of the wounds of the pets can improve the body functions of the pets, promote the wound healing, shorten the wound healing time, improve the autoimmunity of the pets and simultaneously effectively reduce the use amount of antibiotics; and avoiding adverse effects of antibiotics on the active ingredients.

Description

Nutritional supplement for promoting recovery of wounds of pets and preparation method of nutritional supplement

Technical Field

The invention relates to the field of nutritional supplements, in particular to a nutritional supplement for promoting wound healing of pets and a preparation method thereof.

Background

With the acceleration of the pace of life and the improvement of the living standard, more and more people choose to feed pets. Pets, also called companion animals, mainly include cats, dogs, rabbits, squirrels, parrots, etc., refer to animals that require care and feeding by people, form a firm partnership with humans, and provide emotional accommodation for the people. At present, people keep pets mainly to meet the mental needs of the people, the emotion value of the pets to the pet owners is more and more important, and many pets are taken as important members of families by the orphan elderly people.

However, in the course of pet feeding, many pets are injured by various accidents, such as bites, scratches, surgical wounds, etc. After the pet is wounded, the general treatment is to use antibiotics in large doses for a long time until the wound heals; and meanwhile, a certain diet management is matched, so that the nutrition level of the pet is improved. Promoting the healing of wounds and the recovery of bodies of pets. In the prior art, the daily nutrition of the pet is greatly different from the nutrition in the wound recovery period, and aiming at the nutrition supplement for balancing the daily nutrition of the pet, the nutrition supplement is generally focused on the aspects of promoting the appetite of the pet, conditioning the intestines and stomach of the pet, brightening the hair color of the pet and the like, and cannot be suitable for the nutrition requirements of the pet in the wound recovery period.

In the prior art, the recovery period of the wounds of the pets is longer, the common wound healing time is 7-14 days, and the large-scale use of antibiotics can cause irreversible damage to the bodies of the pets, particularly to the livers and kidneys of the pets. The existing nutritional supplements cannot improve the body functions of pets, promote wound healing, shorten the time of wound healing, improve the autoimmunity of pets and simultaneously effectively reduce the use amount of antibiotics.

Meanwhile, the prior art discloses that a compound preparation containing Csad protein is used for the postoperative rehabilitation process of pets, wherein Csad protein can improve the immunity of pets to a certain extent, but in the practical application process, effective components such as Csad protein and the like are greatly influenced by antibiotics, so that the practical beneficial effect is poor.

Disclosure of Invention

In order to solve the technical problems in the prior art, the invention provides the nutritional supplement for promoting the recovery of the wounds of the pets and the preparation method thereof, which can effectively reduce the use amount of antibiotics while improving the physical functions of the pets, promoting the wound healing, shortening the wound healing time and improving the autoimmunity of the pets; and avoiding adverse effects of antibiotics on the active ingredients.

In order to solve the technical problems, the technical scheme adopted by the invention is as follows:

a method for preparing a nutritional supplement for promoting wound healing in pets, comprising the steps of: preparing a first active ingredient, preparing a second active ingredient, compounding, and mixing;

The method for preparing the first active ingredient comprises the steps of extracting pseudo-ginseng powder by ethanol solution and performing rotary evaporation to obtain active ingredient liquid; adding mesoporous silica into 2-3 times volume of active ingredient liquid, heating to 40-45deg.C, maintaining the temperature, stirring, separating solid, and drying to obtain first active ingredient;

The method for preparing the second active ingredient comprises the steps of adding Csad protein into deionized water, uniformly dispersing, stirring at 28-30 ℃, adding hydroxyapatite, and stirring at a constant temperature; stirring and dripping glutaraldehyde; after glutaraldehyde is added dropwise, stirring is continued, standing is carried out, and solid matters are separated; lyophilizing the solid to obtain a second active ingredient;

the compounding method is that a first active ingredient and a second active ingredient are adopted for compounding to prepare a compound;

The mixing method comprises the step of uniformly mixing the compound, coenzyme Q10, fructo-oligosaccharide, saccharicterpenin, dry blowing powder and anhydrous glucose to obtain the nutritional supplement for promoting the recovery of wounds of pets.

Further, in the preparation of the first active ingredient, the pseudo-ginseng is crushed after being trimmed, washed and dried to prepare pseudo-ginseng powder; adding Notoginseng radix powder into 8-10 times of ethanol solution, stirring at normal temperature for 10-12 hr, heating to 60-65deg.C, and reflux extracting for 2-3 hr to obtain extractive solution; filtering the extractive solution, and rotary evaporating to 40-50% of the original volume to obtain active component solution;

the heat preservation stirring time of the mesoporous silica in the active ingredient liquid is 6-10h.

Preferably, in the preparation of the first active ingredient, the volume concentration of the ethanol solution is 55-60%;

The particle size of the mesoporous silica is 8-10 mu m.

Further, in the preparation of the second active ingredient, the weight ratio of Csad protein to deionized water is 1:50-55;

The dropping rate of glutaraldehyde is 0.3-0.5mL/min;

Stirring for 90-120min after glutaraldehyde dripping is completed, and standing for 2-3h.

Preferably, the weight ratio of the hydroxyapatite to the deionized water to the glutaraldehyde is 7-8:100:3-3.5;

The particle size of the hydroxyapatite is 150-200nm.

Further, the compounding method comprises the steps of adding a first active ingredient, a second active ingredient and Tween-60 into deionized water, and uniformly stirring to prepare a first liquid; adding Arabic gum, maltodextrin and sodium carboxymethyl cellulose into deionized water, and stirring for reaction to obtain a second liquid; dropwise adding the first liquid into the second liquid under stirring; and after the first liquid is dripped, spray drying is carried out to obtain the compound.

Preferably, in the composition, the weight ratio of the first active ingredient to the second active ingredient to the Tween-60 to the deionized water in the first liquid is 20-24:4-5:0.25-0.35:100-110;

In the second liquid, the weight ratio of the Arabic gum, the maltodextrin, the sodium carboxymethyl cellulose and the deionized water is 5-6:4-5:1-1.5:100-110;

the volume ratio of the first liquid to the second liquid is 1:2-2.3.

Further, the mixing method comprises the steps of putting the compound, coenzyme Q10 and fructo-oligosaccharide into a mixer, controlling the mixing rotating speed to be 50-70r/min, and uniformly mixing; continuously adding saccharicterpenin, and uniformly mixing; continuously adding the blowing dry powder, and uniformly mixing; continuously adding anhydrous glucose, mixing until the uniformity RSD value is less than 2%, and the water content is less than 6%, so as to obtain the nutritional supplement for promoting the recovery of the wounds of the pets.

Preferably, the weight ratio of the compound to the coenzyme Q10 to the fructo-oligosaccharide to the saccharicterpenin to the dry powder to the anhydrous glucose is 9-12:0.4-1.2:8-14:10-22:1-1.2:150-170.

Wherein Csad protein is one of amino acid decarboxylase, can catalyze the decarboxylation of L-cysteine to produce aminoethylsulfinic acid in vivo, and then produce taurine through dehydrogenation; in this process, csad protein is a key rate limiting enzyme that determines taurine biosynthesis capacity. Csad protein can also catalyze L-aspartic acid to remove alpha carboxyl to produce beta-alanine. Meanwhile, taurine is an active substance for regulating normal physiological activities of organisms, and has wide biological functions of diminishing inflammation, easing pain, maintaining osmotic pressure balance of the organisms, maintaining normal visual functions, regulating calcium balance of cells, reducing blood sugar, regulating nerve conduction, participating in endocrine activities, regulating lipid digestion and absorption, increasing heart contractility, enhancing cell membrane oxidation resistance, protecting myocardial cells, improving organism immunity and the like. Beta-alanine is an important amino acid in animals, and has certain effects and efficacy in promoting muscle growth, enhancing immunity, promoting wound healing and the like.

The saccharicterpenin has stable physical and chemical properties, and has effects of improving animal immunity, improving intestinal health, promoting nutrient absorption, and improving organism antioxidant function.

Coenzyme Q10 has the functions of promoting ATP synthesis, improving intracellular energy supply level, eliminating free radicals in the body, participating in formation of disulfide bonds of proteins and the like, and coenzyme Q10 can also be used as a nonspecific immunity enhancer of organisms to improve the immunity of the organisms.

The pseudo-ginseng has obvious inhibition effect on vascular permeability increase caused by various reasons, has stronger anti-inflammatory effect, has good hemostatic effect, and can obviously shorten bleeding and coagulation time.

The fructo-oligosaccharide has better solubility, high temperature resistance, starch ageing inhibition, alkali resistance, water retention and stability, better mildew resistance and capability of preventing feed from mildew and deterioration. The physiological effects of fructooligosaccharides include: improving intestinal microbiota, promoting intestinal metabolism, enhancing immunity, improving fat metabolism, and promoting mineral absorption.

The blowing dry powder has the characteristics of large specific surface area and good fluidity, has larger adsorption force on other effective components, and has strong hydrophilic performance. The product can effectively control caking discoloration caused by moisture absorption, oxidization and the like, and can accelerate the disintegration of the massive substances.

A nutritional supplement for promoting wound healing in pets is prepared by the above preparation method.

Compared with the prior art, the invention has the beneficial effects that:

(1) The preparation method of the nutritional supplement for promoting the recovery of wounds of pets comprises the steps of preparing a first active ingredient by adopting mesoporous silica to adsorb and combine the active ingredients of pseudo-ginseng; in the preparation of the second active ingredient, the Casd protein is combined and fixed by hydroxyapatite to prepare the second active ingredient; then the first active component and the second active component are used as core materials, and the composite is prepared by coating; then combining the compound with other effective components to prepare the nutritional supplement for promoting the recovery of the wounds of the pets, which can effectively reduce the use amount of antibiotics while improving the physical functions of the pets, promoting the healing of the wounds, shortening the healing time of the wounds and improving the autoimmunity of the pets; and avoiding adverse effects of antibiotics on the active ingredients.

(2) Experiments prove that the nutritional supplement for promoting the recovery of the wounds of the pets disclosed by the invention has the advantages that for simulated operation skin incisions (4 cm) of dogs, the wound healing is promoted, the wound healing time is shortened, and the adverse effect of antibiotics on active ingredients is avoided; the nutritional supplement for promoting the recovery of the wounds of the pets is taken for 5 days, the surfaces of the wounds are dry, no obvious phenomena of redness, liquid seepage and suppuration exist, the standard of stitch removal is achieved, and the healing rate is 100%.

(3) Experiments prove that the nutritional supplement for promoting the recovery of the wounds of the pets can effectively promote the wound healing, shorten the wound healing time and reduce the use amount of antibiotics under the condition of halving the intake dosage of antibiotics aiming at the conditions of scratch, bite and scratch, and the wound healing rate within 5 days of application can reach 98-100 percent compared with the conventional commercial nutritional supplement; compared with the conventional commercial nutritional supplement product, the healing rate within 5 days is improved by 18 percent and 25 percent respectively.

(4) Experiments prove that the nutritional supplement for promoting the recovery of the wounds of the pets can effectively promote the wound healing, shorten the wound healing time and avoid the adverse effect of antibiotics on the effective components in the nutritional supplement under the condition of operation wounds and under the condition of normal intake of conventional dosages of antibiotics, and the wound healing rate within 5 days of application can reach 75-80 percent; compared with the conventional commercial nutritional supplement products, the healing rate within 5 days is respectively improved by 29 percent and 32 percent; the wound healing rate within 10 days of application can reach 98-100%; the healing rate within 10 days is improved by 8% and 12% respectively compared with the conventional commercial nutritional supplement products.

Detailed Description

Specific embodiments of the present invention will now be described in order to provide a clearer understanding of the technical features, objects and effects of the present invention.

Example 1

A method for preparing a nutritional supplement for promoting wound healing in pets, comprising the steps of:

1. Preparation of the first active ingredient

Removing diseased seven and bad seven in the pseudo-ginseng, trimming the residual pseudo-ginseng, washing the pseudo-ginseng after trimming the mixed root, drying until the moisture content is not more than 20wt%, crushing, and sieving with a 40-mesh sieve to obtain pseudo-ginseng powder;

Adding Notoginseng radix powder into 8 times of ethanol solution (volume concentration of 55%), stirring at normal temperature for 10 hr, heating to 60deg.C, and reflux extracting for 2 hr to obtain extractive solution; filtering the extractive solution, and rotary evaporating to 40% of the original volume to obtain active component solution; and (3) adding mesoporous silica into 2 times of volume of active ingredient liquid, heating to 40 ℃, preserving heat and stirring for 6 hours, separating out solid matters, and drying to obtain a first active ingredient.

Wherein the particle size of the mesoporous silica is 8 μm.

2. Preparation of the second active ingredient

Adding Csad proteins into deionized water with the weight being 50 times that of the proteins, uniformly dispersing, stirring and heating to 28 ℃, and preserving heat; then, under the stirring condition, adding hydroxyapatite, and stirring for 3 hours under the heat preservation; stirring and dripping glutaraldehyde at a dripping rate of 0.3 mL/min; after glutaraldehyde is added dropwise, stirring is continued for 90min, and after standing for 2h, solid matters are separated; and freeze-drying the solid to obtain the second active ingredient.

Wherein the weight ratio of the hydroxyapatite to the deionized water to the glutaraldehyde is 7:100:3.

The particle size of the hydroxyapatite was 150nm.

In this embodiment, csad protein is licensed by the institute of microbiology (Beijing) of the national academy of sciences, and Casd protein has been applied for Chinese patent, and the licensed bulletin number is CN107287178B; the Casd protein is specifically represented by SEQ ID NO:1, the purity of the protein composed of the amino acid sequence shown in the formula 1 is more than or equal to 60wt percent.

3. Composite material

Adding the first active ingredient, the second active ingredient and Tween-60 into deionized water, and uniformly stirring to prepare a first liquid; adding Arabic gum, maltodextrin and sodium carboxymethyl cellulose into deionized water, and stirring for reaction to obtain a second liquid; dropwise adding the first liquid into the second liquid under stirring; and after the first liquid is dripped, spray drying is carried out to obtain the compound.

Wherein, in the first liquid, the weight ratio of the first active ingredient to the second active ingredient to Tween-60 to deionized water is 20:4:0.25:100.

In the second liquid, the weight ratio of the Arabic gum, the maltodextrin, the sodium carboxymethyl cellulose and the deionized water is 5:4:1:100.

The volume ratio of the first liquid to the second liquid is 1:2.

4. Mixing

Putting the compound, coenzyme Q10 and fructo-oligosaccharide into a mixer, controlling the mixing rotating speed to be 50r/min, and mixing for 10 min; continuously adding saccharicterpenin, and mixing for 10min at 50 r/min; continuously adding the blowing dry powder, and mixing for 10min at 50 r/min; continuously adding anhydrous glucose, mixing for 10min at 50r/min, detecting the mixing uniformity RSD value of the materials to be less than 2%, and the water content to be less than 6%, so as to obtain the nutritional supplement for promoting the recovery of the wounds of the pets.

Wherein the weight ratio of the compound to the coenzyme Q10 to the fructo-oligosaccharide to the saccharicterpenin to the dry powder to the anhydrous glucose is 9:0.4:8:10:1:170.

The embodiment also provides the nutritional supplement for promoting the recovery of the wounds of the pets, which is prepared by adopting the method.

Example 2

1. Preparation of the first active ingredient

Removing diseased seven and bad seven in the pseudo-ginseng, trimming the residual pseudo-ginseng, washing the pseudo-ginseng after trimming the mixed root, drying until the moisture content is not more than 20wt%, crushing, and sieving with a 60-mesh sieve to obtain pseudo-ginseng powder;

Adding Notoginseng radix powder into 9 times of ethanol solution (volume concentration 56%), stirring at normal temperature for 10.5 hr, heating to 62deg.C, and reflux extracting at constant temperature for 2.5 hr to obtain extractive solution; filtering the extract, and rotationally evaporating to 45% of the original volume to obtain active ingredient liquid; and (3) adding mesoporous silica into 2.5 times of volume of active ingredient liquid, heating to 42 ℃, preserving heat, stirring for 8 hours, separating out solid matters, and drying to obtain a first active ingredient.

Wherein the particle size of the mesoporous silica is 9 μm.

2. Preparation of the second active ingredient

Adding Csad proteins into deionized water with the weight 52 times of that of the proteins, uniformly dispersing, stirring and heating to 29 ℃, and preserving heat; then adding hydroxyapatite under stirring condition, and stirring for 3.5h under heat preservation; stirring and dripping glutaraldehyde at a dripping rate of 0.4 mL/min; after glutaraldehyde is added dropwise, stirring is continued for 100min, and after standing for 2.5h, solid matters are separated; and freeze-drying the solid to obtain the second active ingredient.

Wherein the weight ratio of the hydroxyapatite to the deionized water to the glutaraldehyde is 7.5:100:3.2.

The particle size of the hydroxyapatite was 180nm.

3. Composite material

Wherein, in the first liquid, the weight ratio of the first active ingredient to the second active ingredient to Tween-60 to deionized water is 22:4.5:0.3:105.

In the second liquid, the weight ratio of the Arabic gum, the maltodextrin, the sodium carboxymethyl cellulose and the deionized water is 5.5:4.5:1-.3:105.

The volume ratio of the first liquid to the second liquid is 1:2.1.

4. Mixing

Putting the compound, coenzyme Q10 and fructo-oligosaccharide into a mixer, controlling the mixing rotating speed to be 60r/min, and mixing for 8 min; continuously adding saccharicterpenin, and mixing for 8min at 60 r/min; continuously adding the blowing dry powder, and mixing for 8min at 60 r/min; continuously adding anhydrous glucose, mixing for 8min at 60r/min, detecting the mixing uniformity RSD value of the materials to be less than 2%, and the water content to be less than 6%, thereby obtaining the nutritional supplement for promoting the recovery of the wounds of the pets.

Wherein the weight ratio of the compound to the coenzyme Q10 to the fructo-oligosaccharide to the saccharicterpenin to the dry powder to the anhydrous glucose is 10:0.6:10:14:1:160.

Example 3

1. Preparation of the first active ingredient

Removing diseased seven and bad seven in the pseudo-ginseng, trimming the residual pseudo-ginseng, washing the pseudo-ginseng after trimming the mixed root, drying until the moisture content is not more than 20wt%, crushing, and sieving with a 70-mesh sieve to obtain pseudo-ginseng powder;

Adding Notoginseng radix powder into 9.5 times of ethanol solution (57% volume concentration), stirring at normal temperature for 11 hr, heating to 62deg.C, and reflux extracting at constant temperature for 2.5 hr to obtain extractive solution; filtering the extract, and rotationally evaporating to 45% of the original volume to obtain active ingredient liquid; and (3) adding mesoporous silica into 2.5 times of volume of active ingredient liquid, heating to 42 ℃, preserving heat, stirring for 9 hours, separating out solid matters, and drying to obtain a first active ingredient.

Wherein the particle size of the mesoporous silica is 8.5 μm.

2. Preparation of the second active ingredient

Adding Csad protein into deionized water with the weight being 53 times that of the protein, uniformly dispersing, stirring and heating to 29 ℃, and preserving heat; then adding hydroxyapatite under stirring condition, and stirring for 3.5h under heat preservation; stirring and dripping glutaraldehyde at a dripping rate of 0.4 mL/min; after glutaraldehyde is added dropwise, stirring is continued for 110min, and after standing for 2.5h, solid matters are separated; and freeze-drying the solid to obtain the second active ingredient.

Wherein the weight ratio of the hydroxyapatite to the deionized water to the glutaraldehyde is 7.7:100:3.3.

The particle size of the hydroxyapatite was 160nm.

3. Composite material

Wherein, in the first liquid, the weight ratio of the first active ingredient to the second active ingredient to Tween-60 to deionized water is 23:4.8:0.32:105.

In the second liquid, the weight ratio of the Arabic gum, the maltodextrin, the sodium carboxymethyl cellulose and the deionized water is 5.5:4.7:1.3:105.

The volume ratio of the first liquid to the second liquid is 1:2.2.

4. Mixing

Putting the compound, coenzyme Q10 and fructo-oligosaccharide into a mixer, controlling the mixing rotating speed to be 60r/min, and mixing for 9 min; continuously adding saccharicterpenin, and mixing for 9min at 60 r/min; continuously adding the blowing dry powder, and mixing for 9min at 60 r/min; continuously adding anhydrous glucose, mixing for 9min at 60r/min, detecting the mixing uniformity RSD value of the materials to be less than 2%, and the water content to be less than 6%, thereby obtaining the nutritional supplement for promoting the recovery of the wounds of the pets.

Wherein the weight ratio of the compound to the coenzyme Q10 to the fructo-oligosaccharide to the saccharicterpenin to the dry powder to the anhydrous glucose is 11:0.8:12:18:1.1:155.

Example 4

1. Preparation of the first active ingredient

Removing diseased seven and bad seven in the pseudo-ginseng, trimming the residual pseudo-ginseng, washing the pseudo-ginseng after trimming the mixed root, drying until the moisture content is not more than 20wt%, crushing, and sieving with an 80-mesh sieve to obtain pseudo-ginseng powder;

Adding Notoginseng radix powder into 10 times of ethanol solution (volume concentration 60%), stirring at normal temperature for 12 hr, heating to 65deg.C, and reflux extracting for 3 hr to obtain extractive solution; filtering the extractive solution, and rotary evaporating to 50% of the original volume to obtain active component solution; and (3) adding mesoporous silica into the active ingredient liquid with the volume being 3 times, heating to 45 ℃, preserving heat and stirring for 10 hours, separating out solid matters, and drying to obtain the first active ingredient.

Wherein the particle size of the mesoporous silica is 10 μm.

2. Preparation of the second active ingredient

Adding Csad proteins into deionized water with the weight of 55 times, uniformly dispersing, stirring and heating to 30 ℃, and preserving heat; then, under the stirring condition, adding hydroxyapatite, and stirring for 4 hours under the heat preservation; stirring and dripping glutaraldehyde at a dripping rate of 0.5 mL/min; after glutaraldehyde is added dropwise, stirring is continued for 120min, and after standing for 3h, solid matters are separated; and freeze-drying the solid to obtain the second active ingredient.

Wherein the weight ratio of the hydroxyapatite to the deionized water to the glutaraldehyde is 8:100:3.5.

The particle size of the hydroxyapatite was 200nm.

3. Composite material

In the first liquid, the weight ratio of the first active ingredient to the second active ingredient to Tween-60 to deionized water is 24:5:0.35:110.

In the second liquid, the weight ratio of the Arabic gum, the maltodextrin, the sodium carboxymethyl cellulose and the deionized water is 6:5:1.5:110.

The volume ratio of the first liquid to the second liquid is 1:2.3.

4. Mixing

Putting the compound, coenzyme Q10 and fructo-oligosaccharide into a mixer, controlling the mixing rotating speed to be 70r/min, and mixing for 10 min; continuously adding saccharicterpenin, and mixing for 10min at 70 r/min; continuously adding the blowing dry powder, and mixing for 10min at 70 r/min; continuously adding anhydrous glucose, mixing for 10min at 70r/min, detecting the mixing uniformity RSD value of the materials to be less than 2%, and the water content to be less than 6%, so as to obtain the nutritional supplement for promoting the recovery of the wounds of the pets.

Wherein the weight ratio of the compound to the coenzyme Q10 to the fructo-oligosaccharide to the saccharicterpenin to the dry powder to the anhydrous glucose is 12:1.2:14:22:1.2:150.

Test example 1

2 Pet breeding farms (pet farm A and pet farm B) and 2 pet hospitals (pet hospital A and pet hospital B) are selected as test sites respectively. In the course of the test, a total of 300 pet dogs were tested for wound recovery. Wherein the 300 pet dogs comprise young dogs, minor dogs, adult dogs, aged dogs, and pet dogs during gestation. The 300 pet dogs were randomly grouped and tested, and a test group and a blank control group were set, respectively. The pet dogs in each test group were dosed with the nutritional supplement of example 2 according to the present invention, in addition to the conventional antibiotic intake, by weight, and specifically by adding the nutritional supplement to conventional dog food. Specific:

1) Randomly selecting 20 golden hair dogs in a dog breeding field of 2 dogs, dividing the average into 2 groups of 10 dogs each, and setting the groups as a test group 1 and a blank control group 1; randomly selecting 20 Taidi dogs, and equally dividing the Taidi dogs into 2 groups, wherein 10 dogs in each group are set as a test group 2 and a blank control group 2; a skin incision of 4cm in length was made as required by the procedure on the left or right side of the abdomen of all pet dogs participating in the test.

Wherein test group 1 and test group 2 ingested the nutritional supplement of example 3 of the present invention daily at the same time as the regular intake of antibiotics for 1 feeding cycle at 5 days. Specific intake was divided by body weight of pet dogs: a pet dog weighing less than 2 kg, each intake of 5g, three times a day; a pet dog weighing 2-10 kg, wherein the intake of the pet dog is 10g each time, and the intake is three times a day; the body weight of the pet dogs is greater than 10 kg, and each intake is 15g, three times a day. The blank 1 and the blank 2 were fed only routinely while antibiotics were ingested routinely.

On test day 5 and day 10, wound healing was counted for each group of dogs and the healing rates were calculated for 1 cycle (day 5) and 2 cycles (day 10). The wound healing criteria were: the wound surface is dry, and has no obvious phenomena of redness, swelling, liquid seepage and suppuration. The specific results are shown in the following table:

As can be seen from the table above, compared with the blank control group, the pet dogs in the test group 1 and the test group 2 can obviously promote wound healing and shorten the wound healing time after adopting the nutritional supplement for promoting the wound healing of pets. Wherein, 10 golden dogs in test group 1 healed completely after 5 days; the wound healing condition of 10 golden dogs in the blank control group is not ideal at 5 days, and only 6 golden dogs heal at 10 days. 10 tady dogs in test group 2, and the wound healed completely after 5 days; the wound healing was not ideal for 10 tady dogs in the placebo group at day 5, and only 5 tady dogs had healed at day 10.

2) The test is carried out on the pet dogs with the conditions of scratch, bite and scratch in each pet breeding field and the pet dogs with the surgical wounds in each pet hospital.

Wherein, 100 pet dogs with the conditions are selected in each pet breeding field, and the average is divided into 2 groups (test group and blank control group) of 50 pet dogs; the test group ingests the nutritional supplement of example 3 of the present invention according to the feeding method of the previous test while reducing the antibiotic dose by half; the blank group was ingested with a conventional commercial nutritional supplement (nutritional powder) to promote wound healing, and a conventional dose of antibiotic, based on the ingestion of conventional feed. On test day 5 and day 10, wound healing was counted for each group of dogs and the healing rates were calculated for 1 cycle (day 5) and 2 cycles (day 10). The wound healing criteria were: the wound surface is dry, and has no obvious phenomena of redness, swelling, liquid seepage and suppuration.

100 Pet dogs with the conditions are selected in each pet hospital and divided into 2 groups (test group and blank control group) in average, wherein 50 pet dogs in each group are selected; the test group ingests the nutritional supplement of example 3 of the present invention following the feeding regimen of the previous test; the blank group was fed with a conventional commercial nutritional supplement (nutritional powder) for promoting wound healing after surgery on the basis of a conventional feed. On test day 5 and day 10, wound healing was counted for each group of dogs and the healing rates were calculated for 1 cycle (day 5) and 2 cycles (day 10). The wound healing criteria were: the wound surface is dry, and has no obvious phenomena of redness, swelling, liquid seepage and suppuration. The specific results are shown in the following table:

Compared with the blank control group 3 and the blank control group 4, after the pet dogs in the test group 3 and the test group 4 adopt the nutritional supplement for promoting the recovery of wounds of pets, aiming at the conditions of scratch, bite and scratch, under the condition that the intake dosage of antibiotics is halved, compared with the conventional commercial nutritional supplement, the nutritional supplement for promoting the healing of wounds can effectively promote the healing of wounds, shorten the healing time of wounds and reduce the use amount of antibiotics, and the healing rate of wounds within 5 days of application can reach 98-100%; the healing rate is respectively improved by 18 percent and 25 percent within 5 days compared with the blank control group.

Compared with the blank control group 5 and the blank control group 6, after the pet dogs in the test group 5 and the test group 6 adopt the nutritional supplement for promoting the recovery of the wounds of the pets, aiming at the condition of surgical wounds, under the condition of normal intake of antibiotics at a conventional dosage, compared with the conventional commercial nutritional supplement, the nutritional supplement can effectively promote the wound healing, shorten the time of wound healing, avoid the adverse effect of the antibiotics on the effective components in the nutritional supplement, and the wound healing rate within 5 days of application can reach 75-80%; the healing rate is improved by 29% and 32% respectively in 5 days compared with the blank control group. The wound healing rate within 10 days of application can reach 98-100%; the healing rate is respectively improved by 8 percent and 12 percent within 10 days compared with the blank control group.

The percentages used in the present invention are mass percentages unless otherwise indicated.

Finally, it should be noted that: the foregoing description is only a preferred embodiment of the present invention, and the present invention is not limited thereto, but it is to be understood that modifications and equivalents of some of the technical features described in the foregoing embodiments may be made by those skilled in the art, although the present invention has been described in detail with reference to the foregoing embodiments. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A method of preparing a nutritional supplement for promoting wound healing in pets, comprising the steps of: preparing a first active ingredient, preparing a second active ingredient, compounding, and mixing;

2. The method for preparing a nutritional supplement for promoting wound healing in pets according to claim 1, wherein in the preparation of the first active ingredient, the pseudo-ginseng is crushed after being trimmed, washed with water and dried to prepare pseudo-ginseng powder; adding Notoginseng radix powder into 8-10 times of ethanol solution, stirring at normal temperature for 10-12 hr, heating to 60-65deg.C, and reflux extracting for 2-3 hr to obtain extractive solution; filtering the extractive solution, and rotary evaporating to 40-50% of the original volume to obtain active component solution;

3. The method of claim 2, wherein the first active ingredient is prepared in an ethanol solution having a concentration of 55-60% by volume;

The particle size of the mesoporous silica is 8-10 mu m.

4. The method of claim 2, wherein the second active ingredient comprises Csad protein to deionized water in a weight ratio of 1:50-55;

The dropping rate of glutaraldehyde is 0.3-0.5mL/min;

5. The method of claim 4, wherein the weight ratio of hydroxyapatite, deionized water, glutaraldehyde is 7-8:100:3-3.5;

The particle size of the hydroxyapatite is 150-200nm.

6. The method for preparing a nutritional supplement for promoting wound healing in pets according to claim 1, wherein the compounding method comprises the steps of adding a first active ingredient, a second active ingredient and Tween-60 into deionized water, and uniformly stirring to prepare a first liquid; adding Arabic gum, maltodextrin and sodium carboxymethyl cellulose into deionized water, and stirring for reaction to obtain a second liquid; dropwise adding the first liquid into the second liquid under stirring; and after the first liquid is dripped, spray drying is carried out to obtain the compound.

7. The method of claim 6, wherein the first liquid comprises a first active ingredient, a second active ingredient, tween-60, and deionized water in a weight ratio of 20-24:4-5:0.25-0.35:100-110;

the volume ratio of the first liquid to the second liquid is 1:2-2.3.

8. The method for preparing a nutritional supplement for promoting wound healing of pets according to claim 1, wherein the mixing method comprises the steps of putting the compound, coenzyme Q10 and fructo-oligosaccharide into a mixer, controlling the mixing rotating speed to be 50-70r/min, and uniformly mixing; continuously adding saccharicterpenin, and uniformly mixing; continuously adding the blowing dry powder, and uniformly mixing; continuously adding anhydrous glucose, mixing until the uniformity RSD value is less than 2%, and the water content is less than 6%, so as to obtain the nutritional supplement for promoting the recovery of the wounds of the pets.

9. The method for preparing a nutritional supplement for promoting wound healing in pets according to claim 8, wherein the weight ratio of the compound, coenzyme Q10, fructo-oligosaccharides, saccharicterpenin, dry powder and anhydrous dextrose is 9-12:0.4-1.2:8-14:10-22:1-1.2:150-170.

10. A nutritional supplement for promoting wound healing in pets, prepared by the method of any of claims 1 to 9.