CN117917999A - 色素图像获取方法、色素图像获取装置及色素图像获取程序 - Google Patents

色素图像获取方法、色素图像获取装置及色素图像获取程序 Download PDF

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Publication number
CN117917999A
CN117917999A CN202280057084.5A CN202280057084A CN117917999A CN 117917999 A CN117917999 A CN 117917999A CN 202280057084 A CN202280057084 A CN 202280057084A CN 117917999 A CN117917999 A CN 117917999A
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CN
China
Prior art keywords
fluorescence
image
images
image acquisition
pigment
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Pending
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CN202280057084.5A
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English (en)
Chinese (zh)
Inventor
樋口贵文
池村贤一郎
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Hamamatsu Photonics KK
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Hamamatsu Photonics KK
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Publication of CN117917999A publication Critical patent/CN117917999A/zh
Pending legal-status Critical Current

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/645—Specially adapted constructive features of fluorimeters
    • G01N21/6456—Spatial resolved fluorescence measurements; Imaging
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00—Image analysis
    • G06T7/0002—Inspection of images, e.g. flaw detection
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6419—Excitation at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N2021/6417—Spectrofluorimetric devices
    • G01N2021/6421—Measuring at two or more wavelengths
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00—Features of devices classified in G01N21/00
    • G01N2201/12—Circuits of general importance; Signal processing
    • G01N2201/129—Using chemometrical methods
    • G—PHYSICS
    • G06—COMPUTING OR CALCULATING; COUNTING
    • G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00—Indexing scheme for image analysis or image enhancement
    • G06T2207/10—Image acquisition modality
    • G06T2207/10064—Fluorescence image

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  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Quality & Reliability (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Image Analysis (AREA)
CN202280057084.5A 2021-08-25 2022-07-25 色素图像获取方法、色素图像获取装置及色素图像获取程序 Pending CN117917999A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021-137177 2021-08-25
JP2021137177 2021-08-25
PCT/JP2022/028660 WO2023026742A1 (ja) 2021-08-25 2022-07-25 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Publications (1)

Publication Number Publication Date
CN117917999A true CN117917999A (zh) 2024-04-23

Family

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CN202280057084.5A Pending CN117917999A (zh) 2021-08-25 2022-07-25 色素图像获取方法、色素图像获取装置及色素图像获取程序

Country Status (5)

Country Link
US (1) US20240344985A1 (https=)
EP (1) EP4379359A4 (https=)
JP (2) JP7537029B2 (https=)
CN (1) CN117917999A (https=)
WO (1) WO2023026742A1 (https=)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240259034A1 (en) * 2023-01-26 2024-08-01 Hewlett Packard Enterprise Development Lp Compression of a univariate time-series dataset using motifs
JP7564408B1 (ja) * 2023-02-24 2024-10-08 浜松ホトニクス株式会社 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム
CN120731357A (zh) * 2023-02-24 2025-09-30 浜松光子学株式会社 荧光图像获取方法、荧光图像获取装置、及荧光图像获取程序
EP4653848A1 (en) * 2023-02-24 2025-11-26 Hamamatsu Photonics K.K. Separated image acquisition method, separated image acquisition device, and separated image acquisition program
JP2025177906A (ja) * 2024-05-24 2025-12-05 浜松ホトニクス株式会社 検査装置及び検査方法
JP2026010456A (ja) * 2024-07-09 2026-01-22 浜松ホトニクス株式会社 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム
JP2026036362A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム
JP2026036380A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 生成方法、生成装置、及びプログラム
JP2026036361A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 画像取得方法、装置、及びプログラム
JP2026036378A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム

Family Cites Families (12)

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JP2010243970A (ja) 2009-04-10 2010-10-28 Nikon Corp 共焦点顕微鏡、画像処理装置、および、プログラム
JP5870821B2 (ja) 2012-04-03 2016-03-01 国立研究開発法人理化学研究所 多重蛍光画像の画像解析のための装置、システム、方法、およびプログラム
US8660360B1 (en) 2012-08-03 2014-02-25 Raytheon Company System and method for reduced incremental spectral clustering
CA2931216A1 (en) 2013-12-31 2015-07-09 Ventana Medical Systems, Inc. Systems and methods for spectral unmixing of microscopic images using pixel grouping
JP2018185453A (ja) 2017-04-27 2018-11-22 オリンパス株式会社 顕微鏡システム
JP7197584B2 (ja) * 2017-12-06 2022-12-27 ベンタナ メディカル システムズ， インコーポレイテッド デジタル病理学分析結果の格納および読み出し方法
WO2019150453A1 (en) 2018-01-31 2019-08-08 Nec Corporation Image processing device, image processing method and storage medium
JP2020020791A (ja) 2018-07-24 2020-02-06 ソニー株式会社 情報処理装置、情報処理方法、情報処理システム、およびプログラム
JP7516870B2 (ja) 2019-08-23 2024-07-17 ソニーグループ株式会社 情報処理装置、情報処理方法、プログラム及び情報処理システム
CN110992273B (zh) 2019-11-04 2023-04-18 中国科学院西安光学精密机械研究所 一种自相似性约束的高光谱影像解混方法
JP2021081342A (ja) 2019-11-20 2021-05-27 ソニーグループ株式会社 情報処理システムおよび情報処理装置
CN113221992A (zh) 2021-04-30 2021-08-06 西安交通大学 一种基于l2,1范数的大规模数据快速聚类方法

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Publication number Publication date
JP2024156915A (ja) 2024-11-06
EP4379359A1 (en) 2024-06-05
JP7537029B2 (ja) 2024-08-20
EP4379359A4 (en) 2025-07-16
US20240344985A1 (en) 2024-10-17
JPWO2023026742A1 (https=) 2023-03-02
WO2023026742A1 (ja) 2023-03-02

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