JP7537029B2 - 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム - Google Patents
色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム Download PDFInfo
- Publication number
- JP7537029B2 JP7537029B2 JP2023543753A JP2023543753A JP7537029B2 JP 7537029 B2 JP7537029 B2 JP 7537029B2 JP 2023543753 A JP2023543753 A JP 2023543753A JP 2023543753 A JP2023543753 A JP 2023543753A JP 7537029 B2 JP7537029 B2 JP 7537029B2
- Authority
- JP
- Japan
- Prior art keywords
- fluorescence
- image
- images
- wavelength
- dye
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6456—Spatial resolved fluorescence measurements; Imaging
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T7/00—Image analysis
- G06T7/0002—Inspection of images, e.g. flaw detection
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6419—Excitation at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N2021/6417—Spectrofluorimetric devices
- G01N2021/6421—Measuring at two or more wavelengths
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
- G01N2021/6441—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2201/00—Features of devices classified in G01N21/00
- G01N2201/12—Circuits of general importance; Signal processing
- G01N2201/129—Using chemometrical methods
-
- G—PHYSICS
- G06—COMPUTING OR CALCULATING; COUNTING
- G06T—IMAGE DATA PROCESSING OR GENERATION, IN GENERAL
- G06T2207/00—Indexing scheme for image analysis or image enhancement
- G06T2207/10—Image acquisition modality
- G06T2207/10064—Fluorescence image
Landscapes
- Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Quality & Reliability (AREA)
- Computer Vision & Pattern Recognition (AREA)
- Theoretical Computer Science (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Image Analysis (AREA)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2024130155A JP2024156915A (ja) | 2021-08-25 | 2024-08-06 | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム |
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2021137177 | 2021-08-25 | ||
| JP2021137177 | 2021-08-25 | ||
| PCT/JP2022/028660 WO2023026742A1 (ja) | 2021-08-25 | 2022-07-25 | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム |
Related Child Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024130155A Division JP2024156915A (ja) | 2021-08-25 | 2024-08-06 | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| JPWO2023026742A1 JPWO2023026742A1 (https=) | 2023-03-02 |
| JPWO2023026742A5 JPWO2023026742A5 (https=) | 2024-06-28 |
| JP7537029B2 true JP7537029B2 (ja) | 2024-08-20 |
Family
ID=85323059
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2023543753A Active JP7537029B2 (ja) | 2021-08-25 | 2022-07-25 | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム |
| JP2024130155A Pending JP2024156915A (ja) | 2021-08-25 | 2024-08-06 | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP2024130155A Pending JP2024156915A (ja) | 2021-08-25 | 2024-08-06 | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20240344985A1 (https=) |
| EP (1) | EP4379359A4 (https=) |
| JP (2) | JP7537029B2 (https=) |
| CN (1) | CN117917999A (https=) |
| WO (1) | WO2023026742A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20240259034A1 (en) * | 2023-01-26 | 2024-08-01 | Hewlett Packard Enterprise Development Lp | Compression of a univariate time-series dataset using motifs |
| JP7564408B1 (ja) * | 2023-02-24 | 2024-10-08 | 浜松ホトニクス株式会社 | 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム |
| CN120731357A (zh) * | 2023-02-24 | 2025-09-30 | 浜松光子学株式会社 | 荧光图像获取方法、荧光图像获取装置、及荧光图像获取程序 |
| EP4653848A1 (en) * | 2023-02-24 | 2025-11-26 | Hamamatsu Photonics K.K. | Separated image acquisition method, separated image acquisition device, and separated image acquisition program |
| JP2025177906A (ja) * | 2024-05-24 | 2025-12-05 | 浜松ホトニクス株式会社 | 検査装置及び検査方法 |
| JP2026010456A (ja) * | 2024-07-09 | 2026-01-22 | 浜松ホトニクス株式会社 | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム |
| JP2026036362A (ja) * | 2024-08-20 | 2026-03-05 | 浜松ホトニクス株式会社 | 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム |
| JP2026036380A (ja) * | 2024-08-20 | 2026-03-05 | 浜松ホトニクス株式会社 | 生成方法、生成装置、及びプログラム |
| JP2026036361A (ja) * | 2024-08-20 | 2026-03-05 | 浜松ホトニクス株式会社 | 画像取得方法、装置、及びプログラム |
| JP2026036378A (ja) * | 2024-08-20 | 2026-03-05 | 浜松ホトニクス株式会社 | 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム |
Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010243970A (ja) | 2009-04-10 | 2010-10-28 | Nikon Corp | 共焦点顕微鏡、画像処理装置、および、プログラム |
| JP2013213756A (ja) | 2012-04-03 | 2013-10-17 | Institute Of Physical & Chemical Research | 多重蛍光画像の画像解析のための装置、システム、方法、およびプログラム |
| US8660360B1 (en) | 2012-08-03 | 2014-02-25 | Raytheon Company | System and method for reduced incremental spectral clustering |
| JP2017502286A (ja) | 2013-12-31 | 2017-01-19 | ベンタナ メディカル システムズ, インコーポレイテッド | ピクセルグループ化を使用して微視的画像をスペクトル純化するためのシステムおよび方法 |
| JP2018185453A (ja) | 2017-04-27 | 2018-11-22 | オリンパス株式会社 | 顕微鏡システム |
| JP2020020791A (ja) | 2018-07-24 | 2020-02-06 | ソニー株式会社 | 情報処理装置、情報処理方法、情報処理システム、およびプログラム |
| CN110992273A (zh) | 2019-11-04 | 2020-04-10 | 中国科学院西安光学精密机械研究所 | 一种自相似性约束的高光谱影像解混方法 |
| JP2021036224A (ja) | 2019-08-23 | 2021-03-04 | ソニー株式会社 | 情報処理装置、情報処理方法、プログラム及び情報処理システム |
| JP2021512406A (ja) | 2018-01-31 | 2021-05-13 | 日本電気株式会社 | 画像処理装置、画像処理方法及び画像処理プログラム |
| JP2021081342A (ja) | 2019-11-20 | 2021-05-27 | ソニーグループ株式会社 | 情報処理システムおよび情報処理装置 |
| CN113221992A (zh) | 2021-04-30 | 2021-08-06 | 西安交通大学 | 一种基于l2,1范数的大规模数据快速聚类方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP7197584B2 (ja) * | 2017-12-06 | 2022-12-27 | ベンタナ メディカル システムズ, インコーポレイテッド | デジタル病理学分析結果の格納および読み出し方法 |
-
2022
- 2022-07-25 WO PCT/JP2022/028660 patent/WO2023026742A1/ja not_active Ceased
- 2022-07-25 US US18/293,518 patent/US20240344985A1/en active Pending
- 2022-07-25 JP JP2023543753A patent/JP7537029B2/ja active Active
- 2022-07-25 CN CN202280057084.5A patent/CN117917999A/zh active Pending
- 2022-07-25 EP EP22861033.3A patent/EP4379359A4/en active Pending
-
2024
- 2024-08-06 JP JP2024130155A patent/JP2024156915A/ja active Pending
Patent Citations (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2010243970A (ja) | 2009-04-10 | 2010-10-28 | Nikon Corp | 共焦点顕微鏡、画像処理装置、および、プログラム |
| JP2013213756A (ja) | 2012-04-03 | 2013-10-17 | Institute Of Physical & Chemical Research | 多重蛍光画像の画像解析のための装置、システム、方法、およびプログラム |
| US8660360B1 (en) | 2012-08-03 | 2014-02-25 | Raytheon Company | System and method for reduced incremental spectral clustering |
| JP2017502286A (ja) | 2013-12-31 | 2017-01-19 | ベンタナ メディカル システムズ, インコーポレイテッド | ピクセルグループ化を使用して微視的画像をスペクトル純化するためのシステムおよび方法 |
| JP2018185453A (ja) | 2017-04-27 | 2018-11-22 | オリンパス株式会社 | 顕微鏡システム |
| JP2021512406A (ja) | 2018-01-31 | 2021-05-13 | 日本電気株式会社 | 画像処理装置、画像処理方法及び画像処理プログラム |
| JP2020020791A (ja) | 2018-07-24 | 2020-02-06 | ソニー株式会社 | 情報処理装置、情報処理方法、情報処理システム、およびプログラム |
| JP2021036224A (ja) | 2019-08-23 | 2021-03-04 | ソニー株式会社 | 情報処理装置、情報処理方法、プログラム及び情報処理システム |
| CN110992273A (zh) | 2019-11-04 | 2020-04-10 | 中国科学院西安光学精密机械研究所 | 一种自相似性约束的高光谱影像解混方法 |
| JP2021081342A (ja) | 2019-11-20 | 2021-05-27 | ソニーグループ株式会社 | 情報処理システムおよび情報処理装置 |
| CN113221992A (zh) | 2021-04-30 | 2021-08-06 | 西安交通大学 | 一种基于l2,1范数的大规模数据快速聚类方法 |
Non-Patent Citations (3)
| Title |
|---|
| Introduction to Spectral Imaging and Linear Unmixing,Education in Microscopy and Digital Imaging (online),ZEISS,2021年04月27日,(インターネット)<URL:https://zeiss-campus.magnet.fsu.edu/articles/spectralimaging/introduction.html>,web archive取得日:2021年4月27日、[検索日:2022年8月30日] |
| LUMoS Spectral Unmixing,ImageJ,2019年06月,<URL:https://imagej.net/imagej-wiki-static/LUMoS_Spectral_Unmixing>,初版発行:2019年6月、[検索日:2022年8月30日] |
| MACRE, Tristan D. et al.,Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning,PLOS ONE,2019年12月02日,1-24 |
Also Published As
| Publication number | Publication date |
|---|---|
| JP2024156915A (ja) | 2024-11-06 |
| EP4379359A1 (en) | 2024-06-05 |
| EP4379359A4 (en) | 2025-07-16 |
| CN117917999A (zh) | 2024-04-23 |
| US20240344985A1 (en) | 2024-10-17 |
| JPWO2023026742A1 (https=) | 2023-03-02 |
| WO2023026742A1 (ja) | 2023-03-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JP7537029B2 (ja) | 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム | |
| US11542461B2 (en) | Analysis device | |
| US7953264B2 (en) | Classifying image features | |
| US9147265B2 (en) | System and method for rapid cluster analysis of hyperspectral images | |
| JPWO2023026742A5 (https=) | ||
| JP7566216B1 (ja) | 分離画像取得方法、分離画像取得装置、及び分離画像取得プログラム | |
| JP7564408B1 (ja) | 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム | |
| CN117274236A (zh) | 基于高光谱图像的尿液成分异常检测方法及系统 | |
| EP4653850A1 (en) | Image acquisition device | |
| EP4653849A1 (en) | Fluorescent image acquisition method, fluorescent image acquisition device, and fluorescent image acquisition program | |
| WO2026042345A1 (ja) | 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム | |
| WO2026042344A1 (ja) | 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム | |
| WO2026042403A1 (ja) | 画像取得方法、装置、及びプログラム | |
| Ingold et al. | HAMscope: a snapshot Hyperspectral Autofluorescence Miniscope for real-time molecular imaging | |
| WO2026042343A1 (ja) | 生成方法、生成装置、及びプログラム | |
| Fonseca et al. | Optical Implementation of Learned Spectral Band Selection for Material Classification | |
| Magdaleno-Martinez et al. | Fusing Computed Tomography Image Spectrometry and Convolutional Neural Networks for Biological Tissue Classification |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| A521 | Request for written amendment filed |
Free format text: JAPANESE INTERMEDIATE CODE: A523 Effective date: 20240620 |
|
| A621 | Written request for application examination |
Free format text: JAPANESE INTERMEDIATE CODE: A621 Effective date: 20240620 |
|
| A871 | Explanation of circumstances concerning accelerated examination |
Free format text: JAPANESE INTERMEDIATE CODE: A871 Effective date: 20240620 |
|
| TRDD | Decision of grant or rejection written | ||
| A01 | Written decision to grant a patent or to grant a registration (utility model) |
Free format text: JAPANESE INTERMEDIATE CODE: A01 Effective date: 20240709 |
|
| A61 | First payment of annual fees (during grant procedure) |
Free format text: JAPANESE INTERMEDIATE CODE: A61 Effective date: 20240807 |
|
| R150 | Certificate of patent or registration of utility model |
Ref document number: 7537029 Country of ref document: JP Free format text: JAPANESE INTERMEDIATE CODE: R150 |