JP7537029B2 - 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム - Google Patents

色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム Download PDF

Info

Publication number
JP7537029B2
JP7537029B2 JP2023543753A JP2023543753A JP7537029B2 JP 7537029 B2 JP7537029 B2 JP 7537029B2 JP 2023543753 A JP2023543753 A JP 2023543753A JP 2023543753 A JP2023543753 A JP 2023543753A JP 7537029 B2 JP7537029 B2 JP 7537029B2
Authority
JP
Japan
Prior art keywords
fluorescence
image
images
wavelength
dye
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
JP2023543753A
Other languages
English (en)
Japanese (ja)
Other versions
JPWO2023026742A5 (https=
JPWO2023026742A1 (https=
Inventor
貴文 樋口
賢一郎 池村
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Hamamatsu Photonics KK
Original Assignee
Hamamatsu Photonics KK
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Hamamatsu Photonics KK filed Critical Hamamatsu Photonics KK
Publication of JPWO2023026742A1 publication Critical patent/JPWO2023026742A1/ja
Publication of JPWO2023026742A5 publication Critical patent/JPWO2023026742A5/ja
Priority to JP2024130155A priority Critical patent/JP2024156915A/ja
Application granted granted Critical
Publication of JP7537029B2 publication Critical patent/JP7537029B2/ja
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Images

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6456Spatial resolved fluorescence measurements; Imaging
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T7/00Image analysis
    • G06T7/0002Inspection of images, e.g. flaw detection
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6419Excitation at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N2021/6417Spectrofluorimetric devices
    • G01N2021/6421Measuring at two or more wavelengths
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/6428Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • G01N2021/6441Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks with two or more labels
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2201/00Features of devices classified in G01N21/00
    • G01N2201/12Circuits of general importance; Signal processing
    • G01N2201/129Using chemometrical methods
    • GPHYSICS
    • G06COMPUTING OR CALCULATING; COUNTING
    • G06TIMAGE DATA PROCESSING OR GENERATION, IN GENERAL
    • G06T2207/00Indexing scheme for image analysis or image enhancement
    • G06T2207/10Image acquisition modality
    • G06T2207/10064Fluorescence image

Landscapes

  • Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Optics & Photonics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Quality & Reliability (AREA)
  • Computer Vision & Pattern Recognition (AREA)
  • Theoretical Computer Science (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Image Analysis (AREA)
JP2023543753A 2021-08-25 2022-07-25 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム Active JP7537029B2 (ja)

Priority Applications (1)

Application Number Priority Date Filing Date Title
JP2024130155A JP2024156915A (ja) 2021-08-25 2024-08-06 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
JP2021137177 2021-08-25
JP2021137177 2021-08-25
PCT/JP2022/028660 WO2023026742A1 (ja) 2021-08-25 2022-07-25 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Related Child Applications (1)

Application Number Title Priority Date Filing Date
JP2024130155A Division JP2024156915A (ja) 2021-08-25 2024-08-06 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Publications (3)

Publication Number Publication Date
JPWO2023026742A1 JPWO2023026742A1 (https=) 2023-03-02
JPWO2023026742A5 JPWO2023026742A5 (https=) 2024-06-28
JP7537029B2 true JP7537029B2 (ja) 2024-08-20

Family

ID=85323059

Family Applications (2)

Application Number Title Priority Date Filing Date
JP2023543753A Active JP7537029B2 (ja) 2021-08-25 2022-07-25 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム
JP2024130155A Pending JP2024156915A (ja) 2021-08-25 2024-08-06 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Family Applications After (1)

Application Number Title Priority Date Filing Date
JP2024130155A Pending JP2024156915A (ja) 2021-08-25 2024-08-06 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム

Country Status (5)

Country Link
US (1) US20240344985A1 (https=)
EP (1) EP4379359A4 (https=)
JP (2) JP7537029B2 (https=)
CN (1) CN117917999A (https=)
WO (1) WO2023026742A1 (https=)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20240259034A1 (en) * 2023-01-26 2024-08-01 Hewlett Packard Enterprise Development Lp Compression of a univariate time-series dataset using motifs
JP7564408B1 (ja) * 2023-02-24 2024-10-08 浜松ホトニクス株式会社 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム
CN120731357A (zh) * 2023-02-24 2025-09-30 浜松光子学株式会社 荧光图像获取方法、荧光图像获取装置、及荧光图像获取程序
EP4653848A1 (en) * 2023-02-24 2025-11-26 Hamamatsu Photonics K.K. Separated image acquisition method, separated image acquisition device, and separated image acquisition program
JP2025177906A (ja) * 2024-05-24 2025-12-05 浜松ホトニクス株式会社 検査装置及び検査方法
JP2026010456A (ja) * 2024-07-09 2026-01-22 浜松ホトニクス株式会社 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム
JP2026036362A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム
JP2026036380A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 生成方法、生成装置、及びプログラム
JP2026036361A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 画像取得方法、装置、及びプログラム
JP2026036378A (ja) * 2024-08-20 2026-03-05 浜松ホトニクス株式会社 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム

Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010243970A (ja) 2009-04-10 2010-10-28 Nikon Corp 共焦点顕微鏡、画像処理装置、および、プログラム
JP2013213756A (ja) 2012-04-03 2013-10-17 Institute Of Physical & Chemical Research 多重蛍光画像の画像解析のための装置、システム、方法、およびプログラム
US8660360B1 (en) 2012-08-03 2014-02-25 Raytheon Company System and method for reduced incremental spectral clustering
JP2017502286A (ja) 2013-12-31 2017-01-19 ベンタナ メディカル システムズ, インコーポレイテッド ピクセルグループ化を使用して微視的画像をスペクトル純化するためのシステムおよび方法
JP2018185453A (ja) 2017-04-27 2018-11-22 オリンパス株式会社 顕微鏡システム
JP2020020791A (ja) 2018-07-24 2020-02-06 ソニー株式会社 情報処理装置、情報処理方法、情報処理システム、およびプログラム
CN110992273A (zh) 2019-11-04 2020-04-10 中国科学院西安光学精密机械研究所 一种自相似性约束的高光谱影像解混方法
JP2021036224A (ja) 2019-08-23 2021-03-04 ソニー株式会社 情報処理装置、情報処理方法、プログラム及び情報処理システム
JP2021512406A (ja) 2018-01-31 2021-05-13 日本電気株式会社 画像処理装置、画像処理方法及び画像処理プログラム
JP2021081342A (ja) 2019-11-20 2021-05-27 ソニーグループ株式会社 情報処理システムおよび情報処理装置
CN113221992A (zh) 2021-04-30 2021-08-06 西安交通大学 一种基于l2,1范数的大规模数据快速聚类方法

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7197584B2 (ja) * 2017-12-06 2022-12-27 ベンタナ メディカル システムズ, インコーポレイテッド デジタル病理学分析結果の格納および読み出し方法

Patent Citations (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2010243970A (ja) 2009-04-10 2010-10-28 Nikon Corp 共焦点顕微鏡、画像処理装置、および、プログラム
JP2013213756A (ja) 2012-04-03 2013-10-17 Institute Of Physical & Chemical Research 多重蛍光画像の画像解析のための装置、システム、方法、およびプログラム
US8660360B1 (en) 2012-08-03 2014-02-25 Raytheon Company System and method for reduced incremental spectral clustering
JP2017502286A (ja) 2013-12-31 2017-01-19 ベンタナ メディカル システムズ, インコーポレイテッド ピクセルグループ化を使用して微視的画像をスペクトル純化するためのシステムおよび方法
JP2018185453A (ja) 2017-04-27 2018-11-22 オリンパス株式会社 顕微鏡システム
JP2021512406A (ja) 2018-01-31 2021-05-13 日本電気株式会社 画像処理装置、画像処理方法及び画像処理プログラム
JP2020020791A (ja) 2018-07-24 2020-02-06 ソニー株式会社 情報処理装置、情報処理方法、情報処理システム、およびプログラム
JP2021036224A (ja) 2019-08-23 2021-03-04 ソニー株式会社 情報処理装置、情報処理方法、プログラム及び情報処理システム
CN110992273A (zh) 2019-11-04 2020-04-10 中国科学院西安光学精密机械研究所 一种自相似性约束的高光谱影像解混方法
JP2021081342A (ja) 2019-11-20 2021-05-27 ソニーグループ株式会社 情報処理システムおよび情報処理装置
CN113221992A (zh) 2021-04-30 2021-08-06 西安交通大学 一种基于l2,1范数的大规模数据快速聚类方法

Non-Patent Citations (3)

* Cited by examiner, † Cited by third party
Title
Introduction to Spectral Imaging and Linear Unmixing,Education in Microscopy and Digital Imaging (online),ZEISS,2021年04月27日,(インターネット)<URL:https://zeiss-campus.magnet.fsu.edu/articles/spectralimaging/introduction.html>,web archive取得日:2021年4月27日、[検索日:2022年8月30日]
LUMoS Spectral Unmixing,ImageJ,2019年06月,<URL:https://imagej.net/imagej-wiki-static/LUMoS_Spectral_Unmixing>,初版発行:2019年6月、[検索日:2022年8月30日]
MACRE, Tristan D. et al.,Robust blind spectral unmixing for fluorescence microscopy using unsupervised learning,PLOS ONE,2019年12月02日,1-24

Also Published As

Publication number Publication date
JP2024156915A (ja) 2024-11-06
EP4379359A1 (en) 2024-06-05
EP4379359A4 (en) 2025-07-16
CN117917999A (zh) 2024-04-23
US20240344985A1 (en) 2024-10-17
JPWO2023026742A1 (https=) 2023-03-02
WO2023026742A1 (ja) 2023-03-02

Similar Documents

Publication Publication Date Title
JP7537029B2 (ja) 色素画像取得方法、色素画像取得装置、及び色素画像取得プログラム
US11542461B2 (en) Analysis device
US7953264B2 (en) Classifying image features
US9147265B2 (en) System and method for rapid cluster analysis of hyperspectral images
JPWO2023026742A5 (https=)
JP7566216B1 (ja) 分離画像取得方法、分離画像取得装置、及び分離画像取得プログラム
JP7564408B1 (ja) 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム
CN117274236A (zh) 基于高光谱图像的尿液成分异常检测方法及系统
EP4653850A1 (en) Image acquisition device
EP4653849A1 (en) Fluorescent image acquisition method, fluorescent image acquisition device, and fluorescent image acquisition program
WO2026042345A1 (ja) 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム
WO2026042344A1 (ja) 色素データ取得方法、色素データ取得装置、及び色素データ取得プログラム
WO2026042403A1 (ja) 画像取得方法、装置、及びプログラム
Ingold et al. HAMscope: a snapshot Hyperspectral Autofluorescence Miniscope for real-time molecular imaging
WO2026042343A1 (ja) 生成方法、生成装置、及びプログラム
Fonseca et al. Optical Implementation of Learned Spectral Band Selection for Material Classification
Magdaleno-Martinez et al. Fusing Computed Tomography Image Spectrometry and Convolutional Neural Networks for Biological Tissue Classification

Legal Events

Date Code Title Description
A521 Request for written amendment filed

Free format text: JAPANESE INTERMEDIATE CODE: A523

Effective date: 20240620

A621 Written request for application examination

Free format text: JAPANESE INTERMEDIATE CODE: A621

Effective date: 20240620

A871 Explanation of circumstances concerning accelerated examination

Free format text: JAPANESE INTERMEDIATE CODE: A871

Effective date: 20240620

TRDD Decision of grant or rejection written
A01 Written decision to grant a patent or to grant a registration (utility model)

Free format text: JAPANESE INTERMEDIATE CODE: A01

Effective date: 20240709

A61 First payment of annual fees (during grant procedure)

Free format text: JAPANESE INTERMEDIATE CODE: A61

Effective date: 20240807

R150 Certificate of patent or registration of utility model

Ref document number: 7537029

Country of ref document: JP

Free format text: JAPANESE INTERMEDIATE CODE: R150