CN117882610A - Preparation method of tricholoma giganteum stock - Google Patents
Preparation method of tricholoma giganteum stock Download PDFInfo
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- CN117882610A CN117882610A CN202410283642.0A CN202410283642A CN117882610A CN 117882610 A CN117882610 A CN 117882610A CN 202410283642 A CN202410283642 A CN 202410283642A CN 117882610 A CN117882610 A CN 117882610A
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- 241000302587 Macrocybe gigantea Species 0.000 title claims abstract description 30
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000000463 material Substances 0.000 claims abstract description 29
- 230000001954 sterilising effect Effects 0.000 claims abstract description 26
- 239000002994 raw material Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000000203 mixture Substances 0.000 claims abstract description 20
- 239000001963 growth medium Substances 0.000 claims abstract description 18
- 244000062748 Eupatorium adenophorum Species 0.000 claims abstract description 15
- 241000272525 Anas platyrhynchos Species 0.000 claims abstract description 14
- 210000003608 fece Anatomy 0.000 claims abstract description 14
- 239000010871 livestock manure Substances 0.000 claims abstract description 14
- 239000010802 sludge Substances 0.000 claims abstract description 14
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 13
- 239000010902 straw Substances 0.000 claims abstract description 11
- 238000005303 weighing Methods 0.000 claims abstract description 9
- 235000015099 wheat brans Nutrition 0.000 claims abstract description 9
- 238000002156 mixing Methods 0.000 claims abstract description 7
- 238000011081 inoculation Methods 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 14
- 235000013339 cereals Nutrition 0.000 abstract description 8
- 230000000052 comparative effect Effects 0.000 description 7
- 239000004743 Polypropylene Substances 0.000 description 4
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- 230000002401 inhibitory effect Effects 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
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- 229920001155 polypropylene Polymers 0.000 description 4
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- 230000000694 effects Effects 0.000 description 3
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Mushroom Cultivation (AREA)
Abstract
The invention relates to a preparation method of a tricholoma giganteum stock, which comprises the following steps: s1, preparing a stock culture medium: weighing the following raw material dry materials in percentage by mass: 50-65 parts of eupatorium adenophorum straw, 10-20 parts of sawdust, 6-8 parts of duck manure, 12-18 parts of pond sludge, 4-9 parts of wheat bran and CaCO 3 1 part, adding water into the raw materials, and uniformly mixing until the water content is 60% -65%, thus obtaining a mixture; s2, bagging and sterilizing: putting the mixture obtained in the step S1 into a strain bag, and then sterilizing to obtain a sterilized culture material; s3, inoculating: under the clean environment condition after disinfection, inoculating the tricholoma giganteum mother strain into the obtained sterilized culture material; s4, spawning: and (5) transferring the sterilized culture medium for inoculation into a clean culture room for culture. The invention uses the cheap raw materials such as eupatorium adenophorum, sawdust and the like to replace grains as the strain culture raw materials, so that the raw material cost can be saved; the technical requirement of the whole preparation process is low, and the operation of technicians is facilitated.
Description
Technical Field
The invention relates to the technical field of strain preparation, in particular to a preparation method of tricholoma giganteum stock.
Background
Tricholoma giganteum (Willd.) kultzMacrocybegigantea) Is a rare edible fungus with delicious taste, rich nutrition and high yield, has various effects of preventing and resisting cancer, resisting oxidation, inhibiting hypertension, inhibiting bacteria, inhibiting fungi, inhibiting AIDS virus, improving immunity and the like, and has the effects of benefiting intestines and stomach, resolving phlegm, regulating qi, nourishing liver, tonifying kidney, strengthening body, tonifying deficiency and the like. Can be used as auxiliary dietary therapy for hyperlipidemia, hypertension, hepatitis, rickets, osteomalacia, and tumor. It has effects in nourishing yin, supporting yang, reducing cholesterol, promoting urination, relieving infantile low fever, promoting urination, and strengthening spleen and stomach; has ideal auxiliary treatment function for maintaining beauty, keeping young, protecting health and preventing aging of middle-aged and elderly people. Therefore, it is highly favored.
And the tricholoma giganteum has long shelf life, is not easy to brown and rot in storage and transportation, does not change color or taste after being stored for 30 days at the temperature of 8-12 ℃, is suitable for fresh marketing and processing, and has good development value and application prospect.
The stock of tricholoma giganteum is used for directly cultivating or continuously cultivating and preparing strains of cultivated species after mother strain expansion cultivation, and the stock of tricholoma giganteum is mostly grain strains at present, but the preparation of the grain strains has the following problems:
1. when grain strains are prepared on a large scale, the manufacturing cost is relatively high due to relatively high price of grain crops;
2. the cereal grains need to be steamed at high temperature before being used, the temperature control and the time control of the steaming process are difficult for ordinary technicians, and the cereal grains need to be subjected to abundant experience, so that overripeness or underripeness of the cereal grains are not beneficial to the growth of tricholoma giganteum bacterial hypha, and the seed production is failed.
Disclosure of Invention
Aiming at the technical problems, the invention aims to provide a tricholoma giganteum stock preparation method which is used for reducing the preparation cost of the stock and reducing the technical difficulty of seed production.
The technical aim of the invention is realized by the following technical scheme:
a preparation method of a tricholoma giganteum stock comprises the following steps:
s1, preparing a stock culture medium: weighing the following raw material dry materials in percentage by mass: 50-65 parts of eupatorium adenophorum straw, 10-20 parts of sawdust, 6-8 parts of duck manure, 12-18 parts of pond sludge, 4-9 parts of wheat bran and CaCO 3 1 part, adding water into the raw materials, and uniformly mixing until the water content is 60% -65%, thus obtaining a mixture;
s2, bagging and sterilizing: putting the mixture obtained in the step S1 into a strain bag, and then sterilizing to obtain a sterilized culture material;
s3, inoculating: under the clean environment condition after disinfection, inoculating the tricholoma giganteum mother strain into the obtained sterilized culture material;
s4, spawning: and (5) transferring the sterilized culture medium for inoculation into a clean culture room for culture.
Further, in the step S1, a stock culture medium is prepared: weighing the following raw material dry materials in percentage by mass: 65 parts of eupatorium adenophorum straw, 10 parts of sawdust, 6 parts of duck manure, 12 parts of pond sludge, 6 parts of wheat bran and CaCO 3 1 part of water is added into the raw materials and evenly mixed until the water content is 60 to 65 percent, thus obtaining the mixture.
Further, in the step S2, the sterilization method is as follows: in high-temperature high-pressure equipment, the temperature is 121-126 ℃ and the pressure is 1.1kg/cm 2 -1.5kg/cm 2 Sterilizing for 1.5-2 hr.
Further, the length of the eupatorium adenophorum straws is 0.5cm-0.8cm.
Further, the culture conditions in the step S4 are as follows: the indoor temperature was 30℃and the relative humidity of air was 70%.
In summary, the invention has the following beneficial effects:
1. the invention uses the cheap raw materials such as eupatorium adenophorum, sawdust and the like to replace grains as the strain culture raw materials, so that the raw material cost can be saved;
2. compared with the grain strain manufacturing process which needs to strictly control the temperature and time to ensure that grains reach corresponding maturity, the raw material provided by the invention is simple and convenient in use process, does not need higher technical requirements, and can be operated by conventional technicians;
3. according to the invention, the calcium carbonate is added to buffer the pH value, and meanwhile, the added pond sludge and duck manure can improve the growth speed of hyphae;
4. the invention prepares the eupatorium adenophorum crop into the material of the tricholoma giganteum stock, which can produce economic value while preventing and controlling the eupatorium adenophorum.
Detailed Description
The present invention is specifically described below by way of specific examples, which are merely illustrative of the present invention and not limiting thereof, and modifications which do not creatively contribute to the present embodiment as required may be made by those skilled in the art after reading the present specification, but are protected by patent laws within the scope of claims of the present invention.
In the present invention, materials and reagents used in the present invention are commercially available unless otherwise indicated.
An embodiment I, a method for preparing a tricholoma giganteum stock, comprises the following steps:
s1, preparing a stock culture medium: weighing the following raw material dry materials in percentage by mass: 50 parts of eupatorium adenophorum straws with length of 0.5cm-0.8cm, 20 parts of sawdust, 8 parts of duck manure, 12 parts of pond sludge, 9 parts of wheat bran and CaCO 3 1 part, adding water into the raw materials, uniformly mixing until the water content is 60% -65% (the mixture is manually kneaded, and water flows out but does not drip from the finger joint) to obtain the mixture;
s2, bagging and sterilizing: putting the mixture obtained in the step S1 into a strain bag (17 x 34cm polypropylene strain bag with one end open), and then sterilizing, wherein the sterilization method comprises the following steps: in high-temperature high-pressure equipment, the temperature is 121 ℃ and the pressure is 1.1kg/cm 2 Sterilizing for 2 hours to obtain a sterilized culture material;
s3, inoculating: under the clean environment condition after disinfection, inoculating the tricholoma giganteum mother strain into the obtained sterilized culture material;
s4, spawning: and (3) transferring the sterilized culture material for inoculation into a clean culture room for culture, and keeping the indoor temperature of the culture room at 30 ℃ and the relative air humidity at 70%.
Embodiment II, a tricholoma giganteum stock preparation method comprises the following steps:
s1, preparing a stock culture medium: weighing the raw materials with the following mass fractionsAnd (3) material drying: 60 parts of eupatorium adenophorum straws with length of 0.5cm-0.8cm, 15 parts of sawdust, 6 parts of duck manure, 14 parts of pond sludge, 4 parts of wheat bran and CaCO 3 1 part, adding water into the raw materials, uniformly mixing until the water content is 60% -65% (the mixture is manually kneaded, and water flows out but does not drip from the finger joint) to obtain the mixture;
s2, bagging and sterilizing: putting the mixture obtained in the step S1 into a strain bag (17 x 34cm polypropylene strain bag with one end open), and then sterilizing, wherein the sterilization method comprises the following steps: in high-temperature high-pressure equipment, the temperature is 126 ℃ and the pressure is 1.5kg/cm 2 Sterilizing for 1.5 hours to obtain a sterilized culture material;
s3, inoculating: under the clean environment condition after disinfection, inoculating the tricholoma giganteum mother strain into the obtained sterilized culture material;
s4, spawning: the sterilized culture material is transferred into a clean culture room for culture, the indoor temperature of the culture room is 30 ℃, and the relative air humidity is 70%.
Embodiment three, a tricholoma giganteum stock preparation method, comprising the following steps:
s1, preparing a stock culture medium: weighing the following raw material dry materials in percentage by mass: 65 parts of eupatorium adenophorum straws with length of 0.5cm-0.8cm, 10 parts of sawdust, 6 parts of duck manure, 12 parts of pond sludge, 6 parts of wheat bran and CaCO 3 1 part, adding water into the raw materials, uniformly mixing until the water content is 60% -65% (the mixture is manually kneaded, and water flows out but does not drip from the finger joint) to obtain the mixture;
s2, bagging and sterilizing: putting the mixture obtained in the step S1 into a strain bag (17 x 34cm polypropylene strain bag with one end open), and then sterilizing, wherein the sterilization method comprises the following steps: in high-temperature high-pressure equipment, the temperature is 121 ℃ and the pressure is 1.5kg/cm 2 Sterilizing for 2 hours to obtain a sterilized culture material;
s3, inoculating: under the clean environment condition after disinfection, inoculating the tricholoma giganteum mother strain into the obtained sterilized culture material;
s4, spawning: the sterilized culture material is transferred into a clean culture room for culture, the indoor temperature is 30 ℃, and the relative air humidity is 70%.
Embodiment four, a tricholoma giganteum stock preparation method, comprising the following steps:
s1, preparing a stock culture medium: weighing the following raw material dry materials in percentage by mass: 50 parts of eupatorium adenophorum straws with length of 0.5cm-0.8cm, 19 parts of sawdust, 8 parts of duck manure, 18 parts of pond sludge, 4 parts of wheat bran and CaCO 3 1 part, adding water into the raw materials, uniformly mixing until the water content is 60% -65% (the mixture is manually kneaded, and water flows out but does not drip from the finger joint) to obtain the mixture;
s2, bagging and sterilizing: putting the mixture obtained in the step S1 into a strain bag (17 x 34cm polypropylene strain bag with one end open), and then sterilizing, wherein the sterilization method comprises the following steps: in high-temperature high-pressure equipment, the temperature is 126 ℃ and the pressure is 1.1kg/cm 2 Sterilizing for 2 hours to obtain a sterilized culture material;
s3, inoculating: under the clean environment condition after disinfection, inoculating the tricholoma giganteum mother strain into the obtained sterilized culture material;
s4, spawning: the sterilized culture material is transferred into a clean culture room for culture, the indoor temperature is 30 ℃, and the relative air humidity is 70%.
Comparative example one
The first comparative example differs from the first example in that: the stock culture medium in the step S1 does not contain duck manure.
Comparative example two
The second comparative example differs from the first example in that: the stock culture medium in the step S1 does not contain pond sludge.
Comparative example three
The third comparative example differs from the first example in that: the stock culture medium in the step S1 does not contain pond sludge and duck manure.
10 replicates were set according to the protocol of each example and comparative example, inoculated Tricholoma giganteum strain was New Yu Tg-1, and the mycelium growth rate and the bagging time in Table 1 below were the average of 10 replicates.
TABLE 1 stock growth of Tricholoma giganteum under different treatment methods
As can be seen from the above Table 1, after no duck manure is added to the stock culture medium, the growth rate of hyphae is obviously reduced, the color of the hyphae is not white in example I, the overall density and the thickness of the hyphae are not good in example I, and the bag filling time is increased by 58.3% compared with example I.
After no pond sludge is added into the stock culture medium, the growth speed of hyphae is obviously reduced, compared with the first embodiment, the hyphae have grey and white color, the thickness of the hyphae on the growth vigor is reduced, the growth vigor is worse, and the bag filling time is increased by 55.6 percent compared with the first embodiment.
After no pond sludge and duck manure are added into the stock culture medium, the growth speed of hyphae is obviously reduced, compared with the first embodiment, the hyphae have grey and white color, in terms of growth vigor, the thick density is poor, the hyphae are thinner, the growth vigor is poor, and the bag filling time is increased by 84.3% compared with the first embodiment.
Claims (5)
1. The preparation method of the tricholoma giganteum stock is characterized by comprising the following steps of:
s1, preparing a stock culture medium: weighing the following raw material dry materials in percentage by mass: 50-65 parts of eupatorium adenophorum straw, 10-20 parts of sawdust, 6-8 parts of duck manure, 12-18 parts of pond sludge, 4-9 parts of wheat bran and CaCO 3 1 part, adding water into the raw materials, and uniformly mixing until the water content is 60% -65%, thus obtaining a mixture;
s2, bagging and sterilizing: putting the mixture obtained in the step S1 into a strain bag, and then sterilizing to obtain a sterilized culture material;
s3, inoculating: under the clean environment condition after disinfection, inoculating the tricholoma giganteum mother strain into the obtained sterilized culture material;
s4, spawning: and (5) transferring the sterilized culture medium for inoculation into a clean culture room for culture.
2. The method for preparing the tricholoma giganteum stock according to claim 1, which is characterized in that: s1, preparing a stock culture medium: weighing the following raw material dry materials in percentage by mass: 65 parts of eupatorium adenophorum straw, 10 parts of sawdust, 6 parts of duck manure, 12 parts of pond sludge, 6 parts of wheat bran and CaCO 3 1 part of water is added into the raw materials and evenly mixed until the water content is 60 to 65 percent, thus obtaining the mixture.
3. The method for preparing the tricholoma giganteum stock according to claim 1, which is characterized in that: in the step S2, the sterilization method comprises the following steps: in high-temperature high-pressure equipment, the temperature is 121-126 ℃ and the pressure is 1.1kg/cm 2 -1.5kg/cm 2 Sterilizing for 1.5-2 hr.
4. The method for preparing the tricholoma giganteum stock according to claim 1, which is characterized in that: the length of the eupatorium adenophorum straw is 0.5cm-0.8cm.
5. The method for preparing the tricholoma giganteum stock according to claim 1, which is characterized in that: the culture conditions in the step S4 are as follows: the indoor temperature was 30℃and the relative humidity of air was 70%.
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Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR970014547A (en) * | 1997-01-24 | 1997-04-28 | 구희국 | Compressed body of oyster mushroom cultivation medium and its manufacturing method |
| CA2438232A1 (en) * | 2001-02-20 | 2002-08-29 | Paul Stamets | Delivery systems for mycotechnologies, mycofiltration and mycoremediation |
| WO2003047334A1 (en) * | 2001-12-04 | 2003-06-12 | Money's Foods U.S., Inc. | Polysaccharide mushroom compost supplements |
| CN101627699A (en) * | 2009-08-04 | 2010-01-20 | 郑州大学 | Method for planting edible fungi and producing artificial grass peat by using cabo |
| KR20130098102A (en) * | 2012-02-27 | 2013-09-04 | 박창하 | The method for cultivation various mushrooms using natural substances |
| CN103918482A (en) * | 2014-04-29 | 2014-07-16 | 陕西省微生物研究所 | Method for cultivating tricholoma giganteum |
| CN105037001A (en) * | 2015-08-27 | 2015-11-11 | 四川省农业科学院植物保护研究所 | Pleurotus ostreatus culture medium mainly prepared from Ageratina adenophora |
| WO2020166478A1 (en) * | 2019-02-13 | 2020-08-20 | 住友化学株式会社 | Herbicide composition and weed control method |
| CN117617057A (en) * | 2024-01-04 | 2024-03-01 | 云南省农业科学院药用植物研究所 | Method for cultivating tricholoma giganteum by using rapeseed peels |
-
2024
- 2024-03-13 CN CN202410283642.0A patent/CN117882610A/en active Pending
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR970014547A (en) * | 1997-01-24 | 1997-04-28 | 구희국 | Compressed body of oyster mushroom cultivation medium and its manufacturing method |
| CA2438232A1 (en) * | 2001-02-20 | 2002-08-29 | Paul Stamets | Delivery systems for mycotechnologies, mycofiltration and mycoremediation |
| WO2003047334A1 (en) * | 2001-12-04 | 2003-06-12 | Money's Foods U.S., Inc. | Polysaccharide mushroom compost supplements |
| CN101627699A (en) * | 2009-08-04 | 2010-01-20 | 郑州大学 | Method for planting edible fungi and producing artificial grass peat by using cabo |
| KR20130098102A (en) * | 2012-02-27 | 2013-09-04 | 박창하 | The method for cultivation various mushrooms using natural substances |
| CN103918482A (en) * | 2014-04-29 | 2014-07-16 | 陕西省微生物研究所 | Method for cultivating tricholoma giganteum |
| CN105037001A (en) * | 2015-08-27 | 2015-11-11 | 四川省农业科学院植物保护研究所 | Pleurotus ostreatus culture medium mainly prepared from Ageratina adenophora |
| WO2020166478A1 (en) * | 2019-02-13 | 2020-08-20 | 住友化学株式会社 | Herbicide composition and weed control method |
| CN117617057A (en) * | 2024-01-04 | 2024-03-01 | 云南省农业科学院药用植物研究所 | Method for cultivating tricholoma giganteum by using rapeseed peels |
Non-Patent Citations (9)
| Title |
|---|
| 丁雷;周波;李培;魏建华;: "脱除紫茎泽兰毒素的食用菌菌株筛选初探", 西昌学院学报(自然科学版), no. 04, 20 December 2011 (2011-12-20), pages 15 - 17 * |
| 上官舟建;林汝楷;吴宝芳;: "巨大口蘑生物学特性的研究", 浙江食用菌, no. 01, 15 December 2007 (2007-12-15), pages 123 * |
| 云南省农业科学院: "云南省农业科学院志", vol. 1, 31 December 2006, 云南科技出版社, pages: 342 * |
| 刘祖贵等: "蘑菇露地丰产栽培技术", vol. 1, 31 October 1991, 四川科学技术出版社, pages: 134 * |
| 刘艳华等: "北方食用菌资源及栽培技术", vol. 1, 30 June 2007, 东北林业大学出版社, pages: 218 - 219 * |
| 刘鸿高;王元忠;: "云南大白口蘑子实体人工栽培技术", 江苏农业科学, no. 06, 15 December 2011 (2011-12-15), pages 17 - 19 * |
| 张婷;王元忠;李杰庆;刘鸿高;: "大白口蘑研究进展", 中国食用菌, no. 04, 15 July 2013 (2013-07-15), pages 98 * |
| 张甫安等: "种菇新技术荟萃", vol. 1, 31 March 1991, 中国医药科技出版社, pages: 177 - 178 * |
| 胡清秀;: "立体菌业促进循环农业的发展", 中国农业信息, no. 20, 25 October 2015 (2015-10-25), pages 667 * |
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