CN117814240A - Pesticide composition for preventing and treating cucumber target spot and application thereof - Google Patents
Pesticide composition for preventing and treating cucumber target spot and application thereof Download PDFInfo
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- CN117814240A CN117814240A CN202311635489.5A CN202311635489A CN117814240A CN 117814240 A CN117814240 A CN 117814240A CN 202311635489 A CN202311635489 A CN 202311635489A CN 117814240 A CN117814240 A CN 117814240A
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- fludioxonil
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating cucumber target spot and application thereof. The pesticide composition comprises four active ingredients, wherein the active ingredient A is fludioxonil; the active component B is a methoxy acrylic ester inhibitor; the active ingredient C is a succinate dehydrogenase inhibitor; the active ingredient D is ergosterol inhibitor. The bactericidal composition is applied to the prevention and treatment of cucumber target spot disease, and has obvious functions of reducing and enhancing efficiency and delaying the generation of drug resistance; the bactericidal composition has good effect for preventing and treating target spot disease of crops and is safe to crops.
Description
Technical Field
The invention belongs to the technical field of pesticides, and particularly relates to a pesticide composition for preventing and treating cucumber target spot and application thereof.
Background
The cucumber target spot disease is also called cucumber corynespora leaf spot disease and cucumber brown spot disease, commonly called as yellow spot disease. The pathogenic bacteria of the fungus, namely, the corynesporangium polyspora (corynesporangiicola (B. & C.)) Wei belongs to the genus Cladosporium and is a necrotic plant pathogenic ascomycete, the host is wide in range, can infect more than 530 plants, and causes various plant diseases worldwide, including target leaf spot diseases of cotton, strawberries, tomatoes and the like, and acorn defoliation diseases. The pathogenic bacteria infects the cucumber to form yellow spots in water on the leaves, so that a large amount of cucumber yield reduction is caused, and the cucumber is a destructive fungus disease in a Chinese greenhouse. In recent years, due to the unscientific use of bactericides, the cucumber target spot bacteria have developed drug resistance to bactericides such as carbendazim, azoxystrobin, fluopyram, boscalid and the like, and the prevention and treatment of the diseases become production difficulties. The reasonable bactericide composition can effectively delay the generation of drug resistance, and can expand the bactericidal spectrum to achieve the effects of decrement and synergy, so that the finding of an efficient compound bactericide in the existing bactericide varieties is imperative.
Disclosure of Invention
In order to solve the problems, the invention provides a pesticide composition, which comprises an active ingredient A, B, C, D and auxiliary materials; the active ingredient A is fludioxonil; the active component B is a methoxy acrylic ester inhibitor; the active ingredient C is a succinate dehydrogenase inhibitor; the active ingredient D is ergosterol inhibitor.
Further, the auxiliary materials comprise wetting agents, dispersing agents and fillers.
In some embodiments, the active ingredient B is specifically pyraclostrobin.
In some embodiments, the active ingredient C is specifically one or more of fluxapyroxad hydroxylamine, fluxapyroxad.
In some embodiments, the active ingredient D is specifically one or more of prochloraz, difenoconazole.
In some embodiments, the pesticide composition dosage form is a wettable powder, a water dispersible granule or a soluble powder.
The invention also provides application of the pesticide composition in controlling crop diseases.
Further, the crop disease is cucumber target spot.
The invention has the following beneficial effects:
1. the composition consists of two active ingredients with different action mechanisms, has obvious synergistic effect after being compounded in a certain proportion, improves the control effect, and is beneficial to overcoming and delaying the generation of drug resistance of germs;
2. the dosage is reduced by mixing the medicaments, so that the cost is reduced and the pollution to the environment is reduced.
Detailed Description
Various exemplary embodiments of the invention will now be described in detail, with reference to the examples using conventional methods, unless otherwise indicated, and with reference to reagents, either conventional commercial reagents or reagents configured using conventional methods. The detailed description is not to be taken as limiting, but is to be understood as a more detailed description of certain aspects, features, and embodiments of the invention.
It is to be understood that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. In addition, for numerical ranges in this disclosure, it is understood that each intermediate value between the upper and lower limits of the ranges is also specifically disclosed. Every smaller range between any stated value or stated range, and any other stated value or intermediate value within the stated range, is also encompassed within the invention. The upper and lower limits of these smaller ranges may independently be included or excluded in the range.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. Although only preferred methods and materials are described herein, any methods and materials similar or equivalent to those described herein can be used in the practice or testing of the present invention. All documents mentioned in this specification are incorporated by reference for the purpose of disclosing and describing the methods and/or materials associated with the documents. In case of conflict with any incorporated document, the present specification will control.
It will be apparent to those skilled in the art that various modifications and variations can be made in the specific embodiments of the invention described herein without departing from the scope or spirit of the invention. Other embodiments will be apparent to those skilled in the art from consideration of the specification of the present invention. The specification and examples of the present invention are exemplary only.
As used herein, the terms "comprising," "including," "having," "containing," and the like are intended to be inclusive and mean an inclusion, but not limited to.
The inventor finds that the fludioxonil and any one of other active ingredients of pyraclostrobin, fluxapyroxad, prochloraz and difenoconazole have remarkable synergistic effect on target spot bacteria through a large number of screening tests, and the combination is not just simple addition of the two medicaments (see biological assay examples 1, 2, 3, 4 and 5 for details).
In the following examples, references to "parts by mass" are to be understood in the art as "g", "kg", "mg", "μg", etc., in terms of weight.
The following examples and test examples relate to reagents disclosed below:
fludioxonil (95%, swiss zhengda crop protection limited), prochloraz (98%, shenyang scientific chemical Co., ltd.), pyraclostrobin (97.5%, jiangxi He Yi chemical Co., ltd.), difenoconazole (92%, swiss zhengda crop protection limited). Fluoxapyroxad (98%, pasteur Europe). Fluoxazoyl hydroxylamine (98%, ndFed crop protection Co., switzerland).
Example 1
The example provides a control composition for plant diseases caused by target spot bacteria, which is prepared from fludioxonil and pyraclostrobin according to the mass ratio of 1-9: 9 to 1.
The test method comprises the following steps:
EC 50 is determined by: fludioxonil and pyraclostrobin in a ratio of 1: 9. 1: 4. 3: 7. 2: 3. 1: 1. 3: 2. 7: 3. 4: 1. and 9:1, and serial concentration dilution is carried out, wherein the fludioxonil used in the test has a concentration range of 0.016-10 mug/mL and the pyraclostrobin has a concentration range of 1.28-50 mug/mL. Referring to the agricultural industry standard NY/T1156.2-2006 of the people's republic of China, the inhibition rate of the bactericide on colony diameter is measured by adopting a hypha growth rate method, and a blank control, fludioxonil and pyraclostrobin single-dose treatment and a compound treatment are respectively arranged, wherein each treatment is repeated for 3 times. And (3) taking bacterial cakes with the diameter of 6mm from the edges of bacterial colonies of the pre-cultured cucumber target spot bacteria, placing the bacterial cakes in the center of the culture medium, sealing the films, placing the films in a 28 ℃ incubator for dark culture, measuring the diameters of the bacterial colonies after 6d, and calculating the growth inhibition rate of hyphae of each treatment. Conversion of the hypha growth inhibition ratio into a few-value (y), conversion of the concentration of each treatment of the bactericide (μg/ml) into a number (x), conversion of the concentration of each treatment of the bactericide into a number (x)
Least square method for calculating toxicity equation and concentration EC in inhibition 50 。
The inhibition rate calculation formula is as follows:
synergy measurement: the synergy coefficient (SR) after compounding was evaluated according to the Wadley method. The calculation formula is as follows:
wherein EC is 50( TH) is the theoretical value of bactericide and compounding, EC 50( OB) is an actual measurement value of fludioxonil and the compound bactericide, the synergy coefficient SR is the interaction degree of fludioxonil and the compound bactericide, and A and B respectively represent the proportion of fludioxonil and the compound bactericide in the two compounds. If SR is more than or equal to 1.5, the two have synergistic effect, if 1.5>SR>0.5, it indicates that the two have additive effect, and if SR < 0.5, it indicates that the two exhibit antagonistic effect.
Test results
The test results are shown in Table 1.
TABLE 1 toxicity determination results of fludioxonil and pyraclostrobin on cucumber target spot
The measurement result shows that the ratio of fludioxonil to pyraclostrobin is 1: 4. 3: 7. 2: 3. 1:1, has synergism on the cucumber target spot bacteria, and especially has the proportion of 1:4, the synergy coefficient is the largest.
Example 2
The example provides a control composition for plant diseases caused by target spot bacteria, which is prepared from fludioxonil and difenoconazole according to the mass ratio of 1-9: 9 to 1. The concentration range of fludioxonil is the same as that of example 1, the concentration range of difenoconazole is 0.156-2.5 mug/mL, and the test and statistical methods are the same as those of example 1. The results of the virulence measurements are shown in Table 2.
Table 2 toxicity determination results of fludioxonil and difenoconazole on cucumber target spot disease
The determination result shows that the ratio of fludioxonil to difenoconazole is 3:7, 2: 3. 1: 1. 3: 2. 7: 3. when the ratio of the total amount of the components is 4:1, the total amount of the components has a synergistic effect on the target spot bacteria of the cucumber, and especially when the ratio of the total amount of the components is 1:1, the synergistic effect is more obvious.
Example 3
The example provides a control composition for plant diseases caused by target spot bacteria, which is prepared from fludioxonil and prochloraz according to the mass ratio of 1-9: 9 to 1. The fludioxonil concentration range is the same as in example 1, the prochloraz concentration range is 0.016-10 mug/mL, and the test and statistical methods are the same as in example 1. The results of the virulence measurements are shown in Table 3.
TABLE 3 toxicity determination results of fludioxonil and prochloraz on cucumber target spot disease
The measurement result shows that the ratio of fludioxonil to prochloraz is 1:9, 1: 4. 3: 7. 2: 3. the synergistic effect on the cucumber target spot bacteria is between 1 to 1, and especially at the time of 3 to 7, the synergistic effect is obvious, and the synergistic coefficient is 13.67.
Example 4
The example provides a control composition for plant diseases caused by target spot bacteria, which is prepared from fludioxonil and fluxapyroxad according to the mass ratio of 1-9: 9 to 1. The fludioxonil concentration range is the same as in example 1, and the fluxapyroxad concentration range is 0.22-18 mug/mL. The test and statistical methods are the same as in example 1. The results of the virulence measurements are shown in Table 4.
TABLE 4 toxicity determination results of fludioxonil and fluxapyroxad for cucumber target spot disease
The measurement result shows that the fludioxonil and the fluxapyroxad have remarkable synergism on the cucumber target spot bacteria in the proportion of 1:9-9:1, and the synergism is obvious.
Example 5
The example provides a control composition for plant diseases caused by target spot bacteria, which is prepared from fludioxonil and fluxapyroxad hydroxylamine according to the mass ratio of 1-9: 9 to 1. The fludioxonil concentration range is the same as in example 1, and the fluxapyroxad hydroxylamine concentration range is 0.117-30 μg/mL. The test and statistical methods are the same as in example 1. The results of the virulence measurements are shown in Table 5.
TABLE 5 toxicity determination results of fludioxonil and fluxapyroxad hydroxylamine complex on cucumber target spot disease
The measurement result shows that when the ratio of fludioxonil to fluxapyroxad hydroxylamine is 1:9, the synergistic coefficient of the synergistic effect on the cucumber target spot bacteria is more than 1.5, and the rest ratio is additive effect.
Example 6
The application example provides application of fludioxonil in a compound synergist for preventing and treating cucumber target spot disease.
Field efficacy test of cucumber target spot disease
Test treatment: according to the medicament proportion of the above examples 1-5, the active ingredients of 5% fludioxonil+20% pyraclostrobin, 15% fludioxonil+15% difenoconazole, 6% fludioxonil+14% prochloraz, 14% fludioxonil+6% fluxapyroxad, 4% fludioxonil+36% fluxazoyl hydroxylamine are respectively mixed with 7% sodium methylnaphthalene sulfonate formaldehyde condensate (wetting agent), 0.5% xanthan gum (thickening agent), 0.5% bentonite (thickening agent), 3% agro-emulsion 600# phosphate (dispersing agent), 6% glycerol (antifreeze agent) and water after supplementing 100%, and the corresponding suspending agent is obtained after grinding and/or high-speed shearing. The cucumber field test areas are arranged randomly, and cucumber plants are uniformly sprayed by a hand-operated knapsack sprayer (farmyard brand 3 WBD-18L). Time of first application: 2023, 9, 30, second administration: 2023, 10 and 8. And 5 points are taken from each cell by adopting a random sampling method, 2 plants are respectively investigated at each point, 5 leaves are respectively investigated at each plant, the disease degree of each leaf of each plant is recorded, and the disease index is calculated according to the disease grading standard. Pre-drug investigation was performed 1 time and 1 time after two drug administration. Grading criterion (in leaf): level 0: no disease spots; stage 1: the area of the disease spots accounts for less than 5% of the whole leaf area; 3 stages: the area of the lesion accounts for 6-10% of the whole leaf area; 5 stages: the area of the lesion accounts for 11% -25% of the whole leaf area; 7 stages: the area of the lesion accounts for 26% -50% of the whole leaf area; stage 9: the area of the disease spots accounts for more than 50% of the whole leaf area. According to the disease condition recorded by the leaf damage grading standard, calculating the disease index and the prevention and treatment effect, and performing variance analysis on the test data by using a Duncan new complex polar Difference (DMRT) method. Disease index = Σ (number of leaf of each stage×number of relative stages)/total leaf number of investigation×highest stage value×100. Control effect (%) = [1- ((control region pre-drug disease finger x treatment region post-drug disease finger)/(control region post-drug disease finger x treatment region pre-drug disease finger)) ] ×100
Treatment group 1:30% fludioxonil suspension.
Treatment group 2:300g/L fluxapyroxad suspension.
Treatment group 3:200g/L fluoxastrobin hydroxylamine suspending agent.
Treatment group 4:25% pyraclostrobin suspending agent.
Treatment group 5:50% prochloraz suspending agent.
Treatment group 6:40% difenoconazole suspending agent.
Treatment group 7:20% fludioxonil-fluxapyroxad suspension.
Treatment group 8:40% fludioxonil-fluxapyroxad hydroxylamine suspension.
Treatment group 9:25% fludioxonil pyraclostrobin suspending agent.
Treatment group 10:20% fludioxonil prochloraz suspending agent.
Treatment group 11:30% fludioxonil difenoconazole suspending agent.
Treatment group 12: and (5) comparing with clear water.
The experimental results of the treatment groups 1 to 11 on the prevention and treatment of the cucumber target spot disease are shown in Table 6.
TABLE 6 test results for controlling cucumber target spot
The above embodiments are only illustrative of the preferred embodiments of the present invention and are not intended to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art to the technical solutions of the present invention should fall within the protection scope defined by the claims of the present invention without departing from the design spirit of the present invention.
Claims (8)
1. A pesticide composition, which is characterized by comprising an active ingredient A, B, C, D and auxiliary materials; the active ingredient A is fludioxonil; the active component B is a methoxy acrylic ester inhibitor; the active ingredient C is a succinate dehydrogenase inhibitor; the active ingredient D is ergosterol inhibitor.
2. A pesticide composition as set forth in claim 1 wherein said adjuvant comprises a wetting agent, a dispersing agent and a filler.
3. Pesticide composition according to claim 1, characterized in that the active ingredient B is in particular pyraclostrobin.
4. A pesticide composition as set forth in claim 1, characterized in that the active ingredient C is in particular one or more of fluxapyroxad hydroxylamine, fluxapyroxad.
5. A pesticide composition as set forth in claim 1, characterized in that the active ingredient D is in particular one or more of prochloraz, difenoconazole.
6. A pesticide composition as set forth in claim 1 in the form of wettable powder, water dispersible granule or soluble powder.
7. Use of the pesticidal composition of claims 1-6 for controlling crop diseases.
8. The use according to claim 7, characterized in that the crop disease is cucumber target spot.
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