CN117805424A - Sample analyzer and control method for sample analyzer - Google Patents
Sample analyzer and control method for sample analyzer Download PDFInfo
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- CN117805424A CN117805424A CN202211365728.5A CN202211365728A CN117805424A CN 117805424 A CN117805424 A CN 117805424A CN 202211365728 A CN202211365728 A CN 202211365728A CN 117805424 A CN117805424 A CN 117805424A
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Abstract
The embodiment of the invention discloses a sample analyzer and a control method of the sample analyzer, which can improve the accuracy and detection efficiency of a sample analysis result. The sample analyzer includes: a dispensing mechanism for dispensing a liquid; the reaction cup is used for bearing liquid; the photodetection component is used for detecting liquid in the reaction cup; a controller for acquiring a liquid parameter of the first liquid, wherein the light intensity decreases after the light of the target wavelength passes through the first liquid; controlling the first dispensing mechanism to inject the diluent into the reaction cup and controlling the second dispensing mechanism to inject the first liquid into the reaction cup; controlling a photodetection part to detect mixed liquid of diluent and first liquid in a reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
Description
Cross Reference to Related Applications
The present invention is based on the chinese patent application with application number 202211216872.2, application day 2022, 9 and 30, application name "sample analyzer and control method of sample analyzer", and claims priority of the chinese patent application, the entire contents of which are incorporated herein by reference.
Technical Field
The invention relates to the technical field of medical instruments, in particular to a sample analyzer and a control method of the sample analyzer.
Background
In the use process of the client, the sample analyzer needs to perform basic performance test to judge the performance condition of the daily instrument. Dispensing mechanisms such as sample needles, reagent needles, etc. on sample analyzers are commonly used for liquid sample application, and after prolonged use, may suffer from inaccurate sample application due to equipment wear, chemical residue, etc. However, at present, automatic monitoring cannot be performed on the problem of inaccurate sample adding of the dispensing mechanism, so that not only is a hidden fault with inaccurate sample adding difficult to find, but also the accuracy of a sample analysis result output by the sample analyzer is reduced, and when the problem occurs, the problem needs to be manually examined and positioned from various aspects, so that the detection efficiency is reduced.
Disclosure of Invention
The embodiment of the invention provides a sample analyzer and a control method of the sample analyzer, which can improve the accuracy and detection efficiency of a sample analysis result.
The technical scheme of the invention is realized as follows:
in a first aspect, an embodiment of the present invention provides a sample analyzer, including:
a dispensing mechanism for dispensing a liquid;
The reaction cup is used for bearing liquid;
the photodetection component is used for detecting liquid in the reaction cup;
a controller for acquiring a liquid parameter of the first liquid, wherein the light intensity decreases after the light of the target wavelength passes through the first liquid; controlling a first dispensing mechanism to inject a diluent into the reaction cup and controlling a second dispensing mechanism to inject the first liquid into the reaction cup; controlling the photodetection part to detect mixed liquid of diluent and first liquid in the reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In a second aspect, an embodiment of the present invention provides a method for controlling a sample analyzer, including:
acquiring a liquid parameter of the first liquid, wherein the light intensity is reduced after the light with the target wavelength passes through the first liquid;
controlling a first dispensing mechanism to inject diluent into a reaction cup and controlling a second dispensing mechanism to inject first liquid into the reaction cup;
controlling a photodetection part to acquire a target detection signal of a mixed solution of the diluent and the first liquid in the reaction cup under the target wavelength;
And determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In a third aspect, an embodiment of the present invention provides a sample analyzer, including:
a dispensing mechanism for dispensing a liquid;
the reaction cup is used for bearing liquid;
a target device for performing a first operation on the liquid in the reaction cup;
the photodetection component is used for detecting liquid in the reaction cup;
a controller for controlling the dispensing mechanism to inject a first liquid into the reaction cup, wherein the light intensity of the light with the target wavelength is reduced after the light passes through the first liquid; controlling the light detection component to detect the first liquid in the reaction cup to obtain first absorbance at a target wavelength; controlling the target device to perform the first operation on the liquid in the reaction cup; controlling the light detection component to detect the liquid in the reaction cup after the first operation to obtain the second absorbance at the target wavelength; and determining the working state of the target device according to the first absorbance and the second absorbance.
In a fourth aspect, an embodiment of the present invention provides a method for controlling a sample analyzer, including:
Controlling a dispensing mechanism to inject a first liquid into the reaction cup, wherein the light intensity of the light with the target wavelength is reduced after the light with the target wavelength passes through the first liquid;
controlling a photodetection part to detect first liquid in the reaction cup and obtain first absorbance at a target wavelength;
controlling the target device to perform a first operation on the liquid in the reaction cup;
controlling the light detection component, detecting the first liquid after the first operation, and obtaining second absorbance at the target wavelength;
and determining the working state of the target device according to the first absorbance and the second absorbance.
According to the sample analyzer and the control method of the sample analyzer, provided by the embodiment of the invention, the liquid parameter of the first liquid with the light absorption property for the target wavelength is obtained, the diluent is added into the reaction cup through the first dispensing mechanism, the first liquid is added into the reaction cup through the second dispensing mechanism, the first liquid is diluted in the reaction cup, the mixed liquid containing the diluent and the first liquid is obtained, and the target detection signal of the mixed liquid of the diluent and the first liquid is obtained through detection under the target wavelength. Furthermore, by comparing the liquid parameter before the dilution of the first liquid with the diluted target detection signal, the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism can be determined, so that the automatic monitoring of the sample adding state of the sample analyzer is realized, the problem of inaccurate sample adding can be found in time, the influence on the sample analysis result is reduced, and the accuracy of the sample analysis result is improved. And moreover, the dispensing mechanism with abnormal sample adding state can be rapidly positioned through monitoring sample adding operation, so that the detection efficiency is improved.
Drawings
Fig. 1 is a schematic diagram of a composition structure of a sample analyzer according to an embodiment of the present invention;
fig. 2 is a schematic diagram of a composition structure of a sample analyzer according to an embodiment of the present invention;
FIG. 3 is a graph showing the absorbance change before and after dilution of a first liquid according to the embodiment of the invention;
FIG. 4 is a graph showing the variation of absorbance decay of a first liquid according to an embodiment of the invention;
fig. 5 is a schematic diagram of a second composition structure of a sample analyzer according to an embodiment of the present invention;
FIG. 6 is a flowchart illustrating a first project test cycle according to an embodiment of the present invention;
FIG. 7 is a graph showing absorbance before and after a first agitation according to an embodiment of the present invention;
FIG. 8 is a flowchart illustrating a second project test cycle according to an embodiment of the present invention;
FIG. 9 is a graph showing absorbance curves before and after the second stirring according to the embodiment of the present invention;
FIG. 10 is a flow chart for detecting a water stagnation state of a stirring rod according to an embodiment of the present invention;
FIG. 11 is a flow chart of detecting a sample adding state of a dispensing mechanism and detecting a water stagnation state of a stirring rod according to an embodiment of the present invention;
FIG. 12 is a flowchart illustrating a control method of a sample analyzer according to an embodiment of the present invention;
Fig. 13 is a second flow chart of a control method of a sample analyzer according to an embodiment of the present invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
At present, after a sample analyzer is put into a client for use, a method for daily monitoring long-term stability of the sample analyzer such as sample adding accuracy, water stagnation and the like is lacking. Performance detection of a sample analyzer often requires a professional operator to determine performance conditions of a daily instrument according to a specialized basic performance test procedure. It can be seen that the method is difficult to implement in daily quality control due to higher personnel cost and time cost, so that hidden faults such as inaccurate sample adding of a dispensing mechanism, water stagnation of a stirring rod and the like can be difficult to discover in time, and the accuracy of a sample analysis result on a sample analyzer is reduced. In addition, when the sample analysis result is abnormal, a series of investigation and out-of-control analysis are required to be performed on factors potentially causing abnormality such as source reagents, instruments, irregular operators and the like, so that fault analysis and positioning can be performed, and the detection efficiency is low.
FIG. 1 is an alternative block diagram of a sample analyzer provided in accordance with an embodiment of the present invention, and will be described in conjunction with the steps shown in FIG. 1. As shown in fig. 1, the sample analyzer provided in the embodiment of the present invention includes a sample dispensing mechanism 50 for dispensing a liquid; a reaction cup 151 for carrying a liquid; a photodetection unit 16 for detecting the liquid in the reaction cup 151; a controller 40 for acquiring a liquid parameter of the first liquid, wherein the light intensity decreases after the light of the target wavelength passes through the first liquid; controlling the first dispensing mechanism to inject the diluent into the reaction cup and controlling the second dispensing mechanism to inject the first liquid into the reaction cup 151; the control photodetection part 16 detects the mixed solution of the diluent and the first liquid contained in the reaction cup, and obtains a target detection signal under a target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In some embodiments, referring to fig. 2, the dispensing mechanism 50 includes: a sample dispensing mechanism (second dispensing mechanism) 12 and a reagent dispensing mechanism (first dispensing mechanism) 14; the reaction cup 151 is contained in the reaction part 15; the photodetection means 16 is included in the measurement means; the following is a detailed description.
The sample part is used for carrying a sample to be tested, and the sample is sucked and then provided to the measuring part 30. Referring to fig. 2, in some embodiments the sample assembly may include a sample carrier 11 and a sample dispensing mechanism 12. The sample carrier 11 is for carrying a sample. In some examples, the sample carrier 11 may include a sample distribution module (SDM, sample Delivery Module) and a front end rail; in other examples, such as that shown in fig. 2, the sample carrier 11 may be a sample tray that includes a plurality of sample sites for receiving sample tubes, for example, and the sample tray may be rotated to dispense samples to corresponding locations, such as where sample dispensing mechanism 12 draws samples. The sample dispensing mechanism 12 is used to aspirate and discharge a sample into a reaction cup to be loaded. For example, the sample dispensing mechanism 12 may comprise a sample needle that is moved in two or three dimensions spatially by a two or three dimensional drive mechanism so that the sample needle can be moved to aspirate a sample carried by the sample carrying member 11 and to move to a cuvette to be loaded and discharge the sample to the cuvette.
The reagent component is used for bearing a reagent, and the reagent is supplied to the measuring component after being sucked. Reagent component 20 may include reagent carrier component 13 and reagent dispensing mechanism 14 in some embodiments. The reagent carrying part 13 is for carrying a reagent. In one embodiment, the reagent carrying member 13 may be a reagent disk, which is provided in a disk-like structure and has a plurality of positions for carrying reagent containers, and the reagent carrying member 13 is capable of rotating and driving the reagent containers carried thereby to rotate the reagent containers to a specific position, for example, a position where the reagent is sucked by the reagent dispensing mechanism 14. The number of reagent carrying members 13 may be one or more. The reagent dispensing mechanism 14 is used to aspirate and discharge the reagent into the cuvette to be filled with the reagent. In one embodiment, the reagent dispensing mechanism 14 may include a reagent needle that is moved in two or three dimensions spatially by a two or three dimensional driving mechanism so that the reagent needle can be moved to aspirate the reagent carried by the reagent carrying part 13 and to move to a cuvette to be filled with the reagent and discharge the reagent to the cuvette.
The measuring component is used for carrying out project test on the sample so as to obtain test data of the project. In some embodiments the assay components may include a reaction component 15 and a photometric component 16. The reaction part 15 has at least one place for placing a reaction cup and incubating the reaction liquid in the reaction cup. For example, the reaction part 15 may be a reaction disk, which is arranged in a disk-shaped structure and has one or more placement positions for placing reaction cups, and the reaction disk can rotate and drive the reaction cups in the placement positions to rotate, so as to schedule the reaction cups in the reaction disk and incubate the reaction liquid in the reaction cups. The photodetection unit 16 is used for photodetecting the reaction solution after incubation, and obtaining reaction data of the sample. For example, the photodetection unit 16 detects the luminescence intensity of the reaction solution to be measured, and calculates the concentration of the component to be measured in the sample from the calibration curve. In one embodiment, the photodetection means 16 is separately provided outside the reaction means 15.
In the embodiment of the invention, the light intensity of the light with the target wavelength is reduced after the light passes through the first liquid, that is, the first liquid has light absorption property for the light with the target wavelength. In some embodiments, the first liquid may be a chemical liquid such as a reagent or a pigment having absorbance at a target wavelength. It is understood that a decrease in concentration of the first liquid after dilution results in a decrease in absorbance. Therefore, the first dispensing mechanism to be detected can be controlled to sample the diluent, the second dispensing mechanism to be detected samples the first liquid, the diluent sampled by the first dispensing mechanism and the first liquid sampled by the second dispensing mechanism are utilized to prepare the diluent of the first liquid in the reaction cup, and then the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism is determined by comparing the absorbance change of the light with the target wavelength before and after the dilution of the first liquid.
In some embodiments, the diluent may be water, or other liquid used to dilute the first liquid to reduce its absorbance for light of the target wavelength. The embodiment of the present invention is not limited, and is specifically selected according to the actual situation. The diluent may be pre-placed on the suction site of the first dispensing mechanism, e.g., the diluent may be water from a saline site within the reagent tray; the remaining water may also be pre-stored in the first dispensing means, such as in the reagent needle. The embodiment of the present invention is not limited, and is specifically selected according to the actual situation.
In some embodiments, the target wavelength may comprise a dual wavelength or a single wavelength; the first dispensing mechanism and the second dispensing mechanism are used for dispensing different kinds of liquids, and the first dispensing mechanism may be a reagent needle, the second dispensing mechanism may be a sample needle, and the embodiment of the invention is not limited.
In some embodiments, the volume of diluent used for dilution is greater than the volume of the first liquid, and thus, a first dispensing mechanism with a larger scale, such as a reagent needle, may be used for diluent addition; the addition of the first liquid is performed using a second dispensing mechanism, such as a sample needle, that has a relatively small range. The controller on the sample analyzer is further configured to: controlling the first dispensing mechanism to inject the diluent into the reaction cup and controlling the second dispensing mechanism to inject the first liquid into the reaction cup; controlling a photodetection part to detect mixed liquid of diluent and first liquid in a reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample adding state of the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid. Here, the loading state of the second dispensing mechanism, such as loading error, for injecting the first liquid into the cuvette may be determined by comparing the target detection signal with the liquid parameter of the first liquid.
In the embodiment of the invention, before the first liquid is used for sample adding monitoring, the first liquid can be prepared in advance and the liquid parameters of the first liquid can be measured. In some embodiments, the controller of the sample analyzer is further configured to: controlling a dispensing mechanism to inject a first liquid into the first reaction cup; and controlling the photodetection part to photodetection the first liquid in the first reaction cup to obtain the liquid parameter of the first liquid. Here, the first reaction cup for carrying the first liquid, that is, the stock solution, and the reaction cup for carrying the diluted first liquid are different reaction cups on the sample analyzer. The dispensing mechanism for injecting the first liquid into the first reaction cup may be either a first dispensing mechanism or a second dispensing mechanism, and the embodiment of the present invention is not limited thereto.
In the embodiment of the invention, based on the manufacturing process of the first liquid, the first liquid can be stored in the first reaction cup after the manufacturing is completed, so as to be used for sample feeding monitoring. In some embodiments, the controller of the sample analyzer is further configured to: controlling a dispensing mechanism to inject a first liquid into the first reaction cup; controlling a photodetection part to photodetection the first liquid in the first reaction cup to obtain liquid parameters of the first liquid; controlling the second dispensing mechanism to absorb the first liquid from the first reaction cup and to inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid contained in the second reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In the embodiment of the invention, after the first liquid is injected into the first reaction cup, the controller controls the second dispensing mechanism to sample from the first reaction cup, and the first liquid is sucked into the second reaction cup. And the controller controls the first dispensing mechanism to inject the diluent into the second reaction cup so as to manufacture the diluent of the first liquid in the second reaction cup. Here, the order of the operation of sucking the first liquid from the first cuvette by the second dispensing mechanism and injecting the diluent into the second cuvette by the first dispensing mechanism is not limited. After the first liquid is diluted, the controller controls the light detection component to detect mixed liquid containing the diluted liquid and the first liquid in the second reaction cup, and a target detection signal under a target wavelength is obtained; further, the loading state of the first dispensing mechanism and/or the second dispensing mechanism may be determined based on the target detection signal and the liquid parameter of the first liquid.
In some embodiments, the first liquid may be prepared by injecting a reagent or a pigment, which is previously placed on the reagent site and has known liquid parameters, into the first reaction cup by the dispensing mechanism, so as to monitor the sample loading state. In some embodiments, the sample analyzer further comprises a reagent storage device for storing a reagent. The controller on the sample analyzer is further configured to: controlling a first dispensing mechanism to draw a first liquid from the reagent storage device and to dispense the first liquid into the first reaction cup; controlling the second dispensing mechanism to absorb the first liquid from the first reaction cup and to inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid contained in the second reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
Thus, the first liquid in the first reaction cup is not required to be optically measured, and the known liquid parameters and the target detection signal after the dilution of the first liquid are directly utilized to determine the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism.
In some embodiments, the first liquid may also be made in the first cuvette using a second dispensing mechanism. The controller on the sample analyzer is further configured to: controlling a second dispensing mechanism to suck the first liquid and pour the first liquid into the first reaction cup; controlling a photodetection part to detect the first liquid in the first reaction cup to obtain liquid parameters of the first liquid; then controlling the second dispensing mechanism to absorb the first liquid from the first reaction cup and to inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid contained in the second reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
It can be understood that in the embodiment of the invention, the first liquid for measuring the sample loading state can be manufactured through a flow on the sample analyzer, and a user does not need to specially configure additional raw materials, and the sample analyzer can automatically operate and configure the sample loading state, so that the sample loading state of the dispensing mechanism is automatically detected according to the configured first liquid, and the flexibility and the universality of sample loading state detection on the sample analyzer are improved.
The first liquid is prepared by directly using a reagent or a dye as a raw liquid, and no diluent is added in the preparation process. In some embodiments, if the absorbance of some chemical liquid (such as reagent or pigment) with original concentration is too high, the addition process of the dilution liquid can be added in the first liquid preparation process. Illustratively, the first liquid is obtained by diluting the chemical liquid of the original concentration by performing operations such as adding water by the first dispensing mechanism or the second dispensing mechanism, so that the absorbance value of the first liquid satisfies the absorbance value range measurable by the photometric component.
It should be noted that, in the embodiment of the present invention, the process of preparing the first liquid in the first reaction cup is an optional flow. In some embodiments, the sample of known fluid parameters may also be used as the first fluid, and the sampling and dilution of the first fluid may be accomplished using the relevant sample application procedures in a conventional project test flow of the sample analyzer. The following are provided:
in some embodiments, the sample analyzer further comprises: a sample scheduler, the sample scheduler comprising: a storage area for storing samples, and a scheduling mechanism for scheduling samples; the controller is further configured to: controlling a dispatching mechanism to dispatch the first liquid from the storage area to a liquid sucking position where the second dispensing mechanism sucks the liquid; controlling the second dispensing mechanism to draw the first liquid from the drawing position and pour the first liquid into the second reaction cup, and controlling the first dispensing mechanism to pour the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid contained in the second reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In the embodiment of the invention, the sample adding procedure in the single reagent item test flow of the sample analyzer can comprise the following steps: the controller controls the second dispensing mechanism to draw the sample from the draw site and to inject the sample into the second cuvette and controls the first dispensing mechanism to inject the first reagent into the second cuvette. The controller can control the dispatching mechanism to dispatch the first liquid from the storage area to the sucking position of the second dispensing mechanism to suck the liquid, so that when the sample adding process is performed, the second dispensing mechanism can be controlled to suck the first liquid from the corresponding sucking position to inject the first liquid into the second reaction cup and the first dispensing mechanism is controlled to inject the diluent into the second reaction cup. And determining the sample loading state of the first dispensing mechanism and/or the second dispensing mechanism by the known liquid parameter before dilution and the target detection signal corresponding to the diluted first liquid in the second reaction cup.
In the sample adding process in the dual reagent item test flow, the controller controls the second dispensing mechanism to draw the sample from the drawing position and inject the sample into the second reaction cup, controls the first dispensing mechanism to inject the first reagent into the second reaction cup, and then controls the third dispensing mechanism to inject the second reagent into the second reaction cup after stirring after one sample adding. It can be seen that the step of adding the sample and the first reagent in the single reagent item test procedure is identical to the step of adding the sample and the first reagent in the dual reagent item test procedure. Therefore, the sampling and dilution process of the first liquid can also be performed by using the sample adding process of the dual reagent project test flow. The process is similar to the process of the sample adding procedure in the test flow using the single reagent item, and will not be repeated here.
It can be appreciated that in the embodiment of the invention, the detection of the sample adding state of the dispensing mechanism can be realized by utilizing the existing project test flow, so that the efficiency of detecting the sample adding state is further improved.
In the embodiment of the invention, when the controller on the sample analyzer executes the operation of determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the first liquid parameter and the target detection signal, the controller can determine the absorbance of the first liquid, namely the stock solution, according to the liquid parameter of the first liquid, determine the absorbance corresponding to the diluted first liquid according to the target detection signal, and further determine the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism by comparing the absorbance difference of the first liquid before and after dilution and combining the reaction volume parameters before and after dilution of the liquid in the reaction cup.
In some embodiments, the controller is further configured to: determining a first absorbance of the first liquid according to the liquid parameter of the first liquid and a second absorbance of the mixed liquid according to the target detection signal; acquiring the volume of the first liquid injected into the reaction cup by the second dispensing mechanism, and determining the current diluent sample adding volume corresponding to the first dispensing mechanism according to the volume of the first liquid, the first absorbance and the second absorbance; and determining a first sample adding error or a first sample adding state of the first dispensing mechanism according to the preset diluent sample adding volume corresponding to the current diluent sample adding volume and the first dispensing mechanism.
Here, the liquid parameter of the first liquid includes a light absorption parameter of the first liquid, and the controller may obtain, by converting the liquid parameter of the first liquid, a first light absorbance corresponding to the first liquid. And converting the target detection signal detected by the photodetection component on the diluted first liquid, namely the mixed liquid of the first liquid and the diluted liquid, to obtain the second absorbance corresponding to the mixed liquid.
In some embodiments, based on the structure of the sample analyzer shown in fig. 2, the controller controls the reaction disk 15 to rotate and drives the liquid in the reaction cup 151 to rotate along with the reaction disk, and each time the reaction cup 151 rotates to the light measuring point of the light measuring component 16, the controller controls the light measuring component 16 to perform one-time light measurement on the liquid in the reaction cup, so as to obtain a detection signal of one time point. In some embodiments, the detection signal may be characterized by absorbance. In the sample loading state detection flow, the controller controls the photometric device to perform multiple photometric measurements on the liquid in the reaction cup at multiple photometric points, so that an absorbance curve as shown in fig. 3 can be obtained.
The horizontal axis in the absorbance curve in the embodiment of the present invention is the light measurement point or the time point corresponding to the light measurement point, and the vertical axis is absorbance. As shown in fig. 3, point P3 is the absorbance when the diluent is added to the reaction cup but the first liquid is not added, and the photometric points after point P5 and point P5 are the absorbance of the mixed liquid containing the first liquid and the diluent after the first liquid is added to the reaction cup. Therefore, in some embodiments, at least one absorbance (such as the absorbance at the point P31-P33) may be selected after the point P5 and the point P5 to perform a mean or median treatment, and the difference between the absorbance obtained by the treatment and the absorbance at the point P3 is used as the second absorbance.
In the embodiment of the invention, the controller acquires the liquid amount of the first liquid, namely the volume of the first liquid, injected into the reaction cup by the second injection mechanism. In the reaction process of the reaction cup, the product of the volume of the first liquid injected by the second dispensing mechanism and the first absorbance is equal to the product of the volume of the first liquid diluted in the reaction cup and the second absorbance, and the volume of the first liquid diluted in the reaction cup is the sum of the volume of the first liquid and the volume of the diluted liquid actually injected by the first dispensing mechanism, then the reaction cup comprises:
V S ×A controldey ＝(V S +V R )×A test (1)
in the formula (1), V S For the volume of the first liquid, A controldey For the first absorbance, V R A is the volume of the diluent actually injected into the reaction cup by the first dispensing mechanism to be determined test Is the second absorbance. According to formula (1), formula (2) can be obtained as follows:
thus, through the formula (2), the volume of the diluent actually injected into the reaction cup by the first dispensing mechanism, namely the current diluent sample adding volume corresponding to the first dispensing mechanism, is obtained by calculating the product of the difference value of the first absorbance and the second absorbance and the volume of the first liquid and calculating the ratio of the product to the second absorbance.
In some embodiments, the first loading error of the first dispensing mechanism may be determined based on a preset diluent loading volume corresponding to the current diluent loading volume and the first dispensing mechanism. Here, the preset diluent loading volume may be a calibrated loading volume of the first dispensing mechanism for performing the current diluent loading.
In some embodiments, the loading state of the first dispensing mechanism may be determined based on the first loading error and a preset loading error threshold. Illustratively, determining that the first dispensing mechanism is abnormal in sample addition when the first sample addition error is greater than a preset sample addition error threshold; otherwise, the first dispensing mechanism is determined to be normally loaded.
In some embodiments, the controller is further configured to, based on the determined first absorbance and second absorbance: acquiring the volume of the diluent injected into the reaction cup by the first dispensing mechanism, and determining the current first liquid sample adding volume corresponding to the second dispensing mechanism according to the volume of the diluent, the first absorbance and the second absorbance; and determining a second sample adding error or a second sample adding state of the second dispensing mechanism according to the preset first liquid sample adding volume corresponding to the current first liquid sample adding volume and the second dispensing mechanism.
In the embodiment of the invention, similar to the method for determining the first sample addition error or the first sample addition state, the controller of the sample analyzer may obtain the liquid amount of the diluent injected into the reaction cup by the first dispensing mechanism, that is, the volume of the diluent; and determining the current first liquid sample adding volume corresponding to the second dispensing mechanism according to the known volume of the diluent, the first absorbance and the second absorbance by utilizing the same principle of the formula (1). I.e. to determine the volume of the first liquid that the second dispensing mechanism actually dispenses into the reaction cup. As shown in the formula (3), the calculation process of the formula (3) is not described in detail as follows:
It can be understood that in the embodiment of the invention, the sample adding error is calculated by comparing the absorbance difference before and after the dilution of the first liquid and combining the reaction volume parameter, so that the accuracy of the sample adding state detection is improved, and the accuracy of the sample analysis result on the sample analyzer is further improved.
In some embodiments, when the sample adding error represented by the sample adding state of the first dispensing mechanism or the second dispensing mechanism is greater than a preset error threshold, it is indicated that the sample adding abnormality occurs in the corresponding first dispensing mechanism or second dispensing mechanism, and corresponding prompt can be timely given to the user. The controller controls the built-in or connected prompting equipment of the sample analyzer to prompt in a preset prompting mode; the preset prompting mode comprises at least one of display screen prompting and acousto-optic prompting, but is not limited to the display screen prompting and the acousto-optic prompting.
In some embodiments, the controller may further correct the project test result output by the sample analyzer under the project test period according to the loading state or loading error. Here, the sample analyzer performs the loading state detection process by a loading state detection period, and the loading state detection period and the project test period are different working periods on the sample analyzer.
It can be understood that in the embodiment of the invention, the item test result on the sample analyzer, namely the accuracy of the sample analysis result, is improved by timely prompting the sample addition abnormality and correcting the item test result according to the sample addition error.
In some embodiments, the first liquid stored in the first cuvette may be used multiple times, i.e., the first liquid may be used for at least two loading state detection cycles. Whereas the absorption of the first liquid may decay after a longer period of storage. In some embodiments, the absorbance decay curve of the first liquid may be as shown in fig. 4. To reduce the impact of the first liquid absorbance decay on the loading state detection, in some embodiments, the controller of the sample analyzer is further configured to: the optical detection component is controlled to continuously detect the first liquid in the first reaction cup, and at least two detection signals under the target wavelength are obtained; predicting an estimated signal corresponding to the target detection period of the first liquid in the sample loading state according to at least two detection signals; in the sample adding state target detection period, controlling the second dispensing mechanism to absorb the first liquid from the first reaction cup and inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid contained in the second reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample loading state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the estimated signal.
In the embodiment of the invention, the controller can obtain the signal attenuation trend, such as the signal attenuation curve, of the first liquid by continuously detecting the signal of the first liquid in the first reaction cup. Furthermore, when the sample adding state target detection period is entered, according to the time point of the sample adding state target detection period, the estimated signal corresponding to the sample adding state target detection period of the first liquid can be determined by combining the signal attenuation trend obtained by monitoring the first liquid. Further, the estimated signal is used as the current actual optical signal parameter of the first liquid, and is compared with the target detection signal of the diluted first liquid to determine the sample loading state of the first dispensing mechanism and/or the second dispensing mechanism. Here, the loading state target detection period is one detection period of a plurality of loading state detection periods on the sample analyzer.
In some embodiments, the controller is further configured to: controlling a photodetection part to detect the first liquid in the first reaction cup to obtain an initial detection signal of the first liquid under the target wavelength; the optical measurement component is controlled to continuously detect the first liquid in the first reaction cup to obtain at least two detection signals under the target wavelength, and a correction coefficient is obtained according to the at least two detection signals; in the sample adding state target detection period, controlling a second dispensing mechanism to suck the first liquid from the first reaction cup and inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid contained in the second reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample loading state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal, the initial detection signal of the first liquid and the correction coefficient.
In the embodiment of the invention, the controller can obtain the initial detection signal of the first liquid under the target wavelength by carrying out optical measurement on the initial state of the first liquid. And in the process of storing the first liquid, continuously detecting the first liquid in the first reaction cup at least twice to obtain at least two detection signals under the target wavelength, and obtaining a correction coefficient according to the difference between the at least two detection signals. In this way, the correction detection signal corresponding to the first liquid can be determined according to the initial detection signal and the correction coefficient of the first liquid, so that the correction detection signal is used as the actual liquid parameter of the first liquid, namely the stock solution. And under the condition that a target detection period of the sample adding state is entered, and a target detection signal corresponding to the diluted first liquid is obtained, the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism is determined according to the corrected detection signal and the target detection signal.
In some embodiments, the controller is further configured to: in each sample adding state detection period, controlling the photodetection part to detect the first liquid stored in the first reaction cup to obtain the liquid parameter of the first liquid; controlling the first dispensing mechanism to inject the diluent into the second reaction cup and controlling the second dispensing mechanism to suck the first liquid from the first reaction cup and inject the first liquid into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid contained in the second reaction cup, and obtaining a target detection signal under a target wavelength; and determining the sample loading state of the first dispensing mechanism or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid. Here, in order to reduce the influence of the first liquid absorbance attenuation on the detection of the loading state, the controller may use the photometric signal of the first liquid detected in real time as the first liquid parameter at each detection of the loading state, and further realize the detection of the loading of the dispensing mechanism based on the liquid parameter of the first liquid detected in real time in each detection period of the loading state and the target detection signal of the diluted first liquid.
It can be understood that in the embodiment of the invention, by considering the influence of the absorption decay of the first liquid on the sample-adding detection of the dispensing mechanism, the actual absorption parameter of the first liquid, namely the stock solution, is determined by using methods such as signal value estimation, signal value correction or real-time detection, so that the accuracy of the sample-adding state detection is improved.
In some embodiments, the sample analyzer further comprises: a stirring rod; before the target detection signal of the mixed liquid in the reaction cup is obtained, the controller is further used for controlling the stirring rod to stir the liquid in the reaction cup so that the first liquid and the diluent are fully mixed, and the light absorption is more stable. Therefore, the accuracy of the detected target detection signal can be improved, and the accuracy of the detection of the sample loading state is further improved.
An embodiment of the present invention provides a sample analyzer, as shown in fig. 5, including:
a dispensing mechanism 50 for dispensing a liquid; a reaction cup 151 for carrying a liquid; a target device 50 for performing a first operation on the liquid in the reaction cup 151; a photodetection unit 16 for detecting the liquid in the reaction cup; a controller 40 for controlling the dispensing mechanism 50 to inject the first liquid into the cuvette, wherein the light intensity is reduced after the light of the target wavelength passes through the first liquid; the control light detecting part 16 detects the first liquid in the reaction cup 151 to obtain the first absorbance at the target wavelength; the control target device 50 performs a first operation on the liquid in the reaction cup; controlling the light measuring part 16 to detect the liquid in the reaction cup 151 after the first operation and obtain the second absorbance at the target wavelength; the operating state of the target device 50 is determined based on the first absorbance and the second absorbance. Therefore, the influence of the first operation on the absorbance of the liquid in the reaction cup is determined by comparing the first absorbance and the second absorbance of the liquid in the reaction cup before and after the first operation, and then the working state of the target device is determined.
In some embodiments, the components in fig. 5 may also be implemented with reference to the detailed structures of the related components in fig. 2, which are not described herein.
In some embodiments, the target device comprises: puddler, first operation includes: and the stirring operation, wherein the controller is also used for determining the water stagnation state of the stirring rod according to the first absorbance before the stirring operation and the second absorbance after the stirring operation.
In the embodiment of the invention, the stirring rod on the sample analyzer is cleaned after each use, so that the residual water quantity can be brought into the stirred liquid in the next use, and the water stagnation of the stirring rod occurs. Here, by comparing the absorbance of the first liquid before and after the stirring operation by the stirring rod, the amount of stagnant water carried into the reaction cup by the stirring rod and the state of stagnant water of the stirring rod can be determined.
In some embodiments, the controller is further configured to: under the condition that the water stagnation state is determined to represent the water stagnation of the stirring rod, controlling a built-in or connected prompting device of the sample analyzer, and prompting the water stagnation in a preset prompting mode; the preset prompting mode comprises at least one of display screen prompting and acousto-optic prompting, but is not limited to the display screen prompting and the acousto-optic prompting.
In some embodiments, the controller is further configured to: and under the condition that the water stagnation state is determined to represent the water stagnation of the stirring rod, starting a preset stirring rod maintenance program in the sample analyzer so as to maintain the stirring rod.
In some embodiments, the controller is further configured to: determining the water stagnation amount of the stirring rod according to the first absorbance and the second absorbance; and correcting the project test result of the sample analyzer according to the water stagnation state or the water stagnation amount.
In some embodiments, one project test flow that may be implemented on a sample analyzer may be as shown in FIG. 6. The target device includes: a first stirring rod; the first operation includes: first stirring; in some embodiments, the first stirring rod may be a sample stirring rod. The controller is further configured to: in a first project test period, controlling a first dispensing mechanism to inject a reagent (such as an R1 reagent) into the reaction cup and controlling a second dispensing mechanism to inject a sample into the reaction cup; controlling a first stirring rod to stir the liquid in the reaction cup for the first time; and controlling the light detection component to detect the liquid in the reaction cup after the first stirring to obtain a project test result of the mixed liquid of the sample and the reagent. In some embodiments, the first project test period may be a test period of a single reagent project test.
Accordingly, in the first agitation stagnant water detection period, the controller is further configured to: controlling the first dispensing mechanism to inject the first liquid into the reaction cup, and controlling the second dispensing mechanism not to dispense the liquid into the reaction cup; controlling a photodetection part to detect liquid in the reaction cup and obtain first absorbance at a target wavelength; controlling a first stirring rod to stir the liquid in the reaction cup for the first time; controlling a light detection component to detect liquid in the first stirred reaction cup and obtain second absorbance at a target wavelength; and determining the water stagnation state of the first stirring rod according to the first absorbance and the second absorbance. It can be seen that unlike the first project test period, in the first agitation stagnant water detection period, the second dispensing mechanism does not dispense liquid into the reaction cup, and only the first agitation lever stagnant water has an influence on absorbance of the first liquid after the first agitation. Therefore, the water stagnation state of the first stirring rod can be determined by detecting the change in absorbance before and after the first stirring.
In the embodiment of the invention, according to the product of the first absorbance before the first stirring and the volume of the liquid before the first stirring in the reaction cup, the product of the second absorbance after the first stirring and the volume of the liquid after the first stirring in the reaction cup is equal to the product. Then there are:
A 1 ×V 1 ＝A 2 ×(V 1 +V sw ) (4)
in the formula (4), A 1 A first absorbance prior to the first agitation; a is that 2 A second absorbance after the first stirring; v (V) 1 Adding a first liquid volume, V, of the reaction cup to the second dispensing mechanism sw For the water stagnation amount of the first stirring rod to be determined, V can be calculated by the formula (5) sw The following are provided:
in some embodiments, the change in absorbance of the first liquid in the reaction cup after the first agitation may be as shown in fig. 7. The first agitation in fig. 7 occurs between P3 and P5. The absorbance near the P3 point can be selected as the first absorbance before the first stirring; and selecting a plurality of absorbance near the P5-P7 point to perform average or median treatment, and taking the absorbance as the second absorbance after the first stirring. The embodiment of the present invention is not limited, and is specifically selected according to the actual situation.
In some embodiments, another project test flow that may be implemented on a sample analyzer may be as shown in FIG. 8. The target device further includes: a second stirring rod; the first operation includes: second stirring; the controller is further configured to: in the second project test period, controlling the first dispensing mechanism to inject the first reagent into the reaction cup and controlling the second dispensing mechanism to inject the sample into the reaction cup; controlling a first stirring rod to stir the liquid in the reaction cup for the first time; controlling the third dispensing mechanism to inject a second reagent (such as R2 reagent) into the reaction cup; controlling a second stirring rod to stir the liquid in the reaction cup for the second time; and controlling the light detection component to detect the liquid in the reaction cup, thereby obtaining a project test result. In some embodiments, the second item test period may be a test period of a dual reagent item test.
Correspondingly, the controller is also used for: in the second stirring water stagnation detection period, controlling the first dispensing mechanism or the second dispensing mechanism to inject the first liquid into the reaction cup; controlling a first stirring rod to stir the liquid in the reaction cup for the first time; controlling a photodetection part to detect liquid in the first stirred reaction cup to obtain first absorbance at a target wavelength; the third dispensing mechanism does not dispense, and controls the second stirring rod to stir the liquid in the reaction cup for the second time; controlling the light detection component to detect the liquid in the second stirred reaction cup to obtain second absorbance at the target wavelength; and determining the water stagnation state of the second stirring rod according to the first absorbance and the second absorbance. It can be seen that, unlike the second project test cycle, since the dispensing of the third dispensing mechanism was not performed after the first agitation and before the second agitation, only the second stirring rod was stagnant water that affected the absorbance of the liquid in the reaction cup after the second agitation. Therefore, the operating state of the second stirring rod, that is, the stagnant water state can be determined by detecting the change in absorbance before and after the second stirring.
In the second stirring water retention detection period, the first absorbance before the second stirring after the first stirring corresponds to the second absorbance after the first stirring in the first stirring water retention detection period.
In some embodiments, the change in absorbance of the first liquid in the reaction cup after the second agitation may be as shown in fig. 9. The second agitation in fig. 9 occurs between the photosites P16 and P17. The average value or the median value can be obtained according to the absorbance corresponding to the three light measuring points near P14-P16, so as to obtain the first absorbance before the second stirring after the first stirring; and carrying out average value or median value taking treatment according to the absorbance corresponding to the three measuring points near the P17-P20 to obtain a second absorbance after second stirring.
In the embodiment of the invention, similar to formula (4), in the second stirring stagnant water detection period, as shown in formula (6), the product of the first absorbance before the second stirring after the first stirring and the liquid volume before the second stirring after the first stirring should be equal to the product of the second absorbance after the second stirring and the liquid volume after the second stirring, as follows:
A 1 ×(V 1 +V sw )＝A 2 ×(V 1 +V sw +V Rw ) (6)
wherein A is 1 A is the first absorbance before the first stirring and the second stirring 2 Is the second absorbance after the second stirring, V 1 +V sw +V Rw V is the volume of the liquid after the second stirring sw For the first stirring stagnant water detection period, carrying out stagnant water detection on the first stirring rod to determine the stagnant water quantity of the first stirring rod. V under the condition of not considering the water stagnation amount of the first stirring rod sw May be 0.V (V) Rw Is the stagnant water amount of the second stirring rod to be determined. Based on the formula (6), the stagnant water amount of the second stirring rod can be calculated by the formula (7), as follows:
for example, the stirring rod water stagnation monitoring process of the first stirring water stagnation detection period and the second stirring water stagnation detection period described above may be as shown in fig. 10. First, a chemical liquid having absorbance, such as a dye, is added to the cuvette through an R1 reagent needle (first dispensing mechanism). Here, according to the absorbance value of the chemical liquid, water can be selectively added to the reaction cup through a sample needle (second dispensing mechanism) to dilute the chemical liquid with original concentration to obtain a first liquid; or the chemical liquid in the reaction cup is directly used as the first liquid without adding water. Then, detecting optical signals of the first liquid in the reaction cup to obtain first absorbance before first stirring; first stirring the first liquid in the reaction cup by using a first stirring rod such as a sample stirring rod; detecting optical signals of the stirred liquid in the reaction cup after the first stirring to obtain second absorbance after the first stirring; and determining the water stagnation amount or the water stagnation state of the first stirring rod according to the first absorbance before the first stirring and the second absorbance after the first stirring, and completing the detection of the first stirring water stagnation detection period. Then, the R2 reagent needle is not added with liquid, and the second stirring rod is used for carrying out second stirring on the liquid after the first stirring in the reaction cup; and measuring an optical signal of the liquid after the second stirring to obtain second absorbance after the second stirring. Here, the second absorbance after the first stirring corresponds to the first absorbance before the second stirring. And then can confirm the water stagnation state of the second stirring rod according to the second absorbance after the first stirring and the second absorbance after the second stirring, and finish the detection of the second stirring water stagnation detection period.
In some embodiments, under the condition that the water stagnation amount of the first stirring rod and/or the second stirring rod is determined, the water stagnation state of the first stirring rod and/or the second stirring rod can be judged and pre-warned according to the water stagnation amount of the first stirring rod and/or the second stirring rod and a preset water stagnation amount threshold, and the stirring rod is maintained according to the water stagnation amount or the water stagnation state, or the project test result of the sample analyzer is corrected.
In some embodiments, at least one first stirring rod and at least one second stirring rod are included on the sample analyzer, and illustratively, 3 sample stirring rods and 3 reagent stirring rods may be included. Wherein at least one first stirring rod is used for each first stirring in turn, and at least one second stirring rod is used for each second stirring in turn. Therefore, the controller is used for continuously executing the stirring rod water stagnation detection flow in the embodiment of the invention for a plurality of times, so that the water stagnation state of each stirring rod on the sample analyzer can be monitored.
It can be understood that in the embodiment of the invention, through the absorbance change before and after the stirring rod operates the first liquid, the automatic monitoring of the water stagnation of the stirring rod can be realized, and the water stagnation problem of the stirring rod can be found in time for further early warning or correction, so that the detection accuracy of the sample analyzer and the accuracy of the analysis result are improved.
In some embodiments, the first operation includes injecting the diluent into the cuvette by a target dispensing mechanism, where the target dispensing mechanism may be any one of the first, second, and third dispensing mechanisms described above. The controller is further used for determining the sample adding state of the target dispensing mechanism according to the absorbance of the first liquid in the reaction cup and the absorbance of the mixed liquid containing the first liquid and the diluent in the reaction cup. That is, when the target device is the target dispensing mechanism, the diluting liquid is added to the first liquid by the target dispensing mechanism, and the sample addition state of the target dispensing mechanism can be determined by comparing the change in absorbance of the first liquid before and after dilution. The specific execution flow is consistent with the description of the sample loading state detection flow, and is not repeated here.
In some embodiments, the sample analyzer detection process provided by the embodiment of the present invention may be as shown in fig. 11, and includes:
s1, adding a first liquid into a first reaction cup of a reaction disk by a reagent needle of S1 and R1.
In step S1, the R1 reagent needle corresponds to the first dispensing mechanism. The controller controls the R1 reagent needle to add the first liquid into the first reaction cup in the reaction disk; and controlling the optical measurement component to detect optical signals of the first liquid in the first reaction cup, so as to obtain the first absorbance of the liquid before the sample in the first reaction cup is stirred.
S2, executing sample stirring of the first reaction cup.
In the step S2, the controller controls a sample stirring rod (namely a first stirring rod) to stir the sample (namely first stirring) of the first liquid in the first reaction cup; and after the sample is stirred, controlling the optical measurement component to detect an optical signal of the liquid after the sample is stirred in the first reaction cup, so as to obtain the second absorbance of the liquid after the sample is stirred in the first reaction cup. Thus, by comparing the first absorbance of the liquid before the sample is stirred in the first reaction cup with the second absorbance of the liquid after the sample is stirred in the first reaction cup, the water stagnation state of the sample stirring rod can be determined.
S3, executing reagent stirring of the first reaction cup.
S3, controlling a reagent stirring rod (namely a second stirring rod) by a controller, and stirring the liquid after stirring the sample in the first reaction cup (namely a second stirring rod); and after the reagent is stirred, controlling the light detection component to detect the light signal of the liquid after the reagent is stirred in the first reaction cup, so as to obtain the second absorbance of the liquid after the reagent is stirred in the first reaction cup. Thus, by comparing the second absorbance of the liquid after the sample is stirred in the first reaction cup (i.e., the first absorbance of the liquid before the reagent is stirred in the first reaction cup) with the second absorbance of the liquid after the reagent is stirred in the first reaction cup, the water stagnation state of the reagent stirring rod can be determined.
It should be noted that, according to the actual state detection requirement, only the water stagnation state of the sample stirring rod may be detected, or only the water stagnation state of the reagent stirring rod may be detected. The embodiment of the present invention is not limited, and is specifically selected according to the actual situation.
And S4, adding water into a second reaction cup of the reaction disk by using an R1 reagent needle.
In the step S4, water is equivalent to the diluent; the controller controls the R1 reagent needle to add water to the second reaction cup of the reaction disk.
S5, the sample needle sucks the liquid in the first reaction cup and adds the liquid into the second reaction cup.
In step S5, the sample needle corresponds to the second dispensing mechanism. The controller controls the sample needle to suck the liquid after reagent stirring from the first reaction cup and to inject the liquid into the second reaction cup.
S6, executing sample stirring of the second reaction cup.
And S6, controlling a sample stirring rod by the controller to stir the sample of the mixed liquid in the second reaction cup. In some embodiments, the loading state of the sample needle and/or the R1 reagent needle may be determined by optical signal detection after performing sample agitation of the second cuvette, as follows:
the controller controls the light measuring component to detect the light signal of the liquid after the sample in the second reaction cup is stirred, and the light signal is used as the absorbance of the mixed liquid in the second reaction cup. Here, the absorbance of the mixed liquid in the second cuvette corresponds to the target detection signal corresponding to the diluted first liquid in the above-described embodiment. In this way, after the reagent in the step S3 is stirred, the second absorbance of the liquid after the reagent is stirred in the first reaction cup is used as the liquid parameter of the first liquid, and the liquid parameter of the first liquid is compared with the target detection signal corresponding to the diluted first liquid, namely, the absorbance difference of the first liquid before and after dilution is compared, so that the sample adding state of the sample needle and/or the R1 reagent needle is determined.
And S7, executing reagent stirring of the second reaction cup.
And S7, controlling a reagent stirring rod by the controller, and stirring the reagent for the mixed liquid after the sample in the second reaction cup is stirred. In some embodiments, the loading state of the sample needle and/or the R1 reagent needle may also be determined by optical signal detection after reagent agitation of the second cuvette is performed, as follows:
the controller controls the light detection component to detect the light signal of the liquid after the reagent is stirred in the second reaction cup, and the light signal is used as the absorbance of the mixed liquid in the second reaction cup, namely the target detection signal corresponding to the diluted first liquid. Further, by the same method, the sample loading state of the sample needle and/or the R1 reagent needle is determined by comparing the second absorbance (the liquid parameter of the first liquid) of the liquid after stirring of the reagent in the first cuvette obtained in step S3 with the target detection signal corresponding to the diluted first liquid.
In some embodiments, the sample analyzer may invoke the detection flow of the working state of the target device in the embodiment of the present invention at a fixed period, perform permutation analysis on the monitored data according to the time dimension, and timely alarm, prompt or correct the abnormal state of the sample analyzer. Such as: when the sample analyzer runs for a period of time, a certain item is calibrated on a certain day, and after the quality control test, the working state detection flow of the embodiment of the invention finds that the stirring rod is abnormal in water stagnation or the sample adding mechanism is abnormal, so that an alarm prompt can be given in time and result correction can be carried out according to the water stagnation or the sample adding error. Or when the currently detected water stagnation amount or sample adding error does not reach the threshold value, the time that the water stagnation amount or sample adding error exceeds the threshold value can be estimated through simulating calibration change, so that a user is informed of actions such as stirring rod and sample adding needle maintenance or inspection in advance, the quality requirement is met, or data are provided for problem investigation when faults occur later.
The embodiment of the invention provides a control method of a sample analyzer, which corresponds to the sample analyzer shown in fig. 1, and as shown in fig. 12, the method comprises S101-S104, as follows:
s101, acquiring a liquid parameter of the first liquid, wherein the light intensity is reduced after the light with the target wavelength passes through the first liquid.
S102, controlling the first dispensing mechanism to inject the diluent into the reaction cup and controlling the second dispensing mechanism to inject the first liquid into the reaction cup.
S103, controlling the photodetection part to acquire a target detection signal of the mixed liquid of the diluent and the first liquid in the reaction cup under the target wavelength.
S104, determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In some embodiments, before the obtaining the liquid parameter of the first liquid, the control method further includes:
controlling the dispensing mechanism to inject the first liquid into the first reaction cup; and controlling the photodetection component to carry out photodetection on the first liquid in the first reaction cup to obtain the liquid parameter of the first liquid.
In some embodiments, the control method further comprises:
controlling the dispensing mechanism to inject the first liquid into the first reaction cup; controlling the photodetection part to carry out photodetection on the first liquid in the first reaction cup to obtain liquid parameters of the first liquid;
Controlling the second dispensing mechanism to draw the first liquid from the first reaction cup and to inject the first liquid into a second reaction cup and controlling the first dispensing mechanism to inject a diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In some embodiments, the sample analyzer further comprises a reagent storage device for storing a reagent; after the liquid parameter of the first liquid is obtained, the control method further comprises:
controlling the first dispensing mechanism to draw the first liquid from the reagent storage device and to dispense the first liquid into a first reaction cup;
controlling the second dispensing mechanism to draw the first liquid from the first reaction cup and to inject the first liquid into the second reaction cup and controlling the first dispensing mechanism to inject the diluent into the second reaction cup;
controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength;
And determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In some embodiments, the sample analyzer further comprises: a sample scheduler, the sample scheduler comprising: a storage area for storing samples, and a scheduling mechanism for scheduling samples; after the liquid parameter of the first liquid is obtained, the control method further comprises:
controlling the dispatching mechanism to dispatch the first liquid from the storage area to a liquid sucking position where the second dispensing mechanism sucks the liquid;
controlling the second dispensing mechanism to draw the first liquid from the drawing position to be injected into a second reaction cup and controlling the first dispensing mechanism to inject diluent into the second reaction cup;
controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength;
and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In some embodiments, after the obtaining the liquid parameter of the first liquid, the control method further includes:
Controlling the second dispensing mechanism to suck the first liquid and pour the first liquid into the first reaction cup; controlling the photodetection part to detect the first liquid in the first reaction cup to obtain liquid parameters of the first liquid;
controlling the second dispensing mechanism to draw the first liquid from the first reaction cup and to inject the first liquid into a second reaction cup and controlling the first dispensing mechanism to inject a diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In some embodiments, the volume of the diluent is greater than the volume of the first liquid; after the liquid parameter of the first liquid is obtained, the control method further comprises:
controlling the first dispensing mechanism to inject a diluent into the reaction cup and controlling the second dispensing mechanism to inject the first liquid into the reaction cup; controlling the photodetection part to detect mixed liquid of diluent and first liquid in the reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
In some embodiments, after the obtaining the liquid parameter of the first liquid, the control method further includes:
determining a first absorbance of the first liquid according to the liquid parameter of the first liquid and a second absorbance of the mixed liquid according to the target detection signal;
acquiring the volume of the first liquid injected into the reaction cup by the second dispensing mechanism, and determining the current diluent sample adding volume corresponding to the first dispensing mechanism according to the volume of the first liquid, the first absorbance and the second absorbance;
determining a first sample adding error or a first sample adding state of the first dispensing mechanism according to the current diluent sample adding volume and a preset diluent sample adding volume corresponding to the first dispensing mechanism;
and/or the number of the groups of groups,
acquiring the volume of the diluent injected into the reaction cup by the first dispensing mechanism, and determining the current first liquid sample adding volume corresponding to the second dispensing mechanism according to the volume of the diluent, the first absorbance and the second absorbance;
and determining a second sample adding error or a second sample adding state of the second dispensing mechanism according to the current first liquid sample adding volume and the preset first liquid sample adding volume corresponding to the second dispensing mechanism.
In some embodiments, the control method further comprises:
controlling the photodetection part to continuously detect the first liquid in the first reaction cup to obtain at least two detection signals under the target wavelength; predicting estimated signals corresponding to the target detection period of the first liquid in the sample loading state according to the at least two detection signals;
in a sample adding state target detection period, controlling the second dispensing mechanism to absorb the first liquid from the first reaction cup and inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; determining the sample loading state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the estimated signal;
or,
controlling the photodetection part to detect the first liquid in the first reaction cup to obtain an initial detection signal of the first liquid under the target wavelength; the photodetection part is controlled to continuously detect the first liquid in the first reaction cup, at least two detection signals under the target wavelength are obtained, and a correction coefficient is obtained according to the at least two detection signals;
In a sample adding state target detection period, controlling the second dispensing mechanism to suck the first liquid from the first reaction cup and inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal, the initial detection signal of the first liquid and the correction coefficient;
or,
in each sample adding state detection period, controlling the photodetection part to detect the first liquid stored in the first reaction cup to obtain the liquid parameter of the first liquid; controlling the first dispensing mechanism to inject diluent into a second reaction cup and controlling the second dispensing mechanism to suck the first liquid from the first reaction cup and inject the first liquid into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; determining the sample adding state of the first dispensing mechanism or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid; the first liquid stored in the first reaction cup is used for at least two sample adding state detection periods.
In some embodiments, after the determining the loading state of the first dispensing mechanism and/or the second dispensing mechanism, the control method further comprises:
when the sample adding state of the first dispensing mechanism or the second dispensing mechanism represents that the sample adding error is larger than a preset error threshold, controlling a built-in or connected prompting device of the sample analyzer to prompt in a preset prompting mode; the preset prompting mode comprises at least one of display screen prompting and acousto-optic prompting;
and/or the number of the groups of groups,
and correcting the project test result of the sample analyzer according to the sample adding state or the sample adding error.
In some embodiments, the sample analyzer further comprises: a stirring rod; before the target detection signal of the mixed liquid in the reaction cup is obtained, the control method further comprises the following steps:
and controlling the stirring rod to stir the liquid in the reaction cup so as to mix the first liquid and the diluent.
The embodiment of the invention provides a control method of a sample analyzer, which corresponds to the sample analyzer shown in fig. 5, and as shown in fig. 13, the method comprises S201-S204, as follows: the control method comprises the following steps:
S201, controlling a dispensing mechanism to inject a first liquid into the reaction cup, wherein the light intensity of the light with the target wavelength is reduced after the light passes through the first liquid;
s202, controlling a light measurement component to detect first liquid in the reaction cup, and obtaining first absorbance at a target wavelength;
s203, controlling the target device to perform a first operation on the liquid in the reaction cup; controlling the light detection component to detect the first liquid after the first operation to obtain second absorbance at the target wavelength;
s204, determining the working state of the target device according to the first absorbance and the second absorbance.
In some embodiments, the target device comprises: a puddler, the first operation comprising: and (3) stirring operation, wherein the working state of the target device is determined according to the first absorbance and the second absorbance, and the method comprises the following steps:
and determining the water stagnation state of the stirring rod according to the first absorbance before the stirring operation and the second absorbance after the stirring operation.
In some embodiments, after the determining the water stagnation state of the stirring rod, the control method further comprises:
under the condition that the water stagnation state is determined to represent the water stagnation of the stirring rod, controlling a built-in or connected prompting device of the sample analyzer to perform water stagnation prompting in a preset prompting mode; the preset prompting mode comprises at least one of display screen prompting and acousto-optic prompting;
And/or the number of the groups of groups,
under the condition that the water stagnation state represents water stagnation of the stirring rod, starting a preset stirring rod maintenance program in the sample analyzer so as to maintain the stirring rod;
and/or the number of the groups of groups,
determining the water stagnation amount of the stirring rod according to the first absorbance and the second absorbance; and correcting the project test result of the sample analyzer according to the water stagnation state or the water stagnation amount.
In some embodiments, the target device comprises: a first stirring rod; the first operation includes: first stirring; the control method further includes:
in a first project test period, controlling a first dispensing mechanism to inject a reagent into the reaction cup and controlling a second dispensing mechanism to inject a sample into the reaction cup; controlling the first stirring rod to stir the liquid in the reaction cup for the first time; controlling the light detection component, detecting the liquid in the first stirred reaction cup, and obtaining a project test result of the mixed liquid of the sample and the reagent;
controlling the first dispensing mechanism to inject a first liquid into the reaction cup in a first stirring water stagnation detection period, wherein the second dispensing mechanism does not dispense the liquid into the reaction cup; controlling the photodetection part to detect liquid in the reaction cup, and obtaining first absorbance at the target wavelength; controlling the first stirring rod to stir the liquid in the reaction cup for the first time; controlling the photodetection part to detect the liquid in the reaction cup after the first stirring to obtain the second absorbance under the target wavelength; and determining the water stagnation state of the first stirring rod according to the first absorbance and the second absorbance.
In some embodiments, the target device further comprises: a second stirring rod; the first operation includes: second stirring; the control method further includes:
in a second project test period, controlling a first dispensing mechanism to inject a first reagent into the reaction cup and controlling a second dispensing mechanism to inject a sample into the reaction cup; controlling the first stirring rod to stir the liquid in the reaction cup for the first time; controlling a third dispensing mechanism to inject a second reagent into the reaction cup; controlling the second stirring rod to stir the liquid in the reaction cup for the second time; controlling the light measuring component, and detecting liquid in the reaction cup to obtain a project test result;
controlling the first dispensing mechanism or the second dispensing mechanism to inject the first liquid into the reaction cup in a second stirring water stagnation detection period; controlling a first stirring rod to stir the liquid in the reaction cup for the first time; controlling the light detection component to detect liquid in the first stirred reaction cup, and obtaining first absorbance at the target wavelength; the third dispensing mechanism does not dispense, and controls the second stirring rod to stir the liquid in the reaction cup for the second time; controlling the light detection component to detect liquid in the second stirred reaction cup, and obtaining second absorbance at the target wavelength; and determining the water stagnation state of the second stirring rod according to the first absorbance and the second absorbance.
In some embodiments, the first operation includes injecting a diluent into the reaction cup by the target dispensing mechanism, and determining the working state of the target device according to the first absorbance and the second absorbance includes:
and determining the sample adding state of the target dispensing mechanism according to the absorbance of the first liquid in the reaction cup and the absorbance of the mixed liquid containing the first liquid and the diluent in the reaction cup.
It should be noted that the description of the method embodiment above is similar to the description of the apparatus embodiment above, with similar advantageous effects as the apparatus embodiment. For technical details not disclosed in the method embodiments of the present invention, please refer to the description of the apparatus embodiments of the present invention for understanding.
It will be apparent to those skilled in the art that embodiments of the present invention may be provided as a method, apparatus, system, or computer program product. Accordingly, the present invention may take the form of a hardware embodiment, a software embodiment, or an embodiment combining software and hardware aspects. Furthermore, the present invention may take the form of a computer program product embodied on one or more computer-usable storage media (including, but not limited to, magnetic disk storage, optical storage, and the like) having computer-usable program code embodied therein.
The present invention is described with reference to flowchart illustrations and/or block diagrams of methods, apparatus (systems) and computer program products according to embodiments of the invention. It will be understood that each flow and/or block of the flowchart illustrations and/or block diagrams, and combinations of flows and/or blocks in the flowchart illustrations and/or block diagrams, can be implemented by computer program instructions. These computer program instructions may be provided to a processor of a general purpose computer, special purpose computer, embedded processor, or other programmable data processing apparatus to produce a machine, such that the instructions, which execute via the processor of the computer or other programmable data processing apparatus, create means for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be stored in a computer-readable memory that can direct a computer or other programmable data processing apparatus to function in a particular manner, such that the instructions stored in the computer-readable memory produce an article of manufacture including instruction means which implement the function specified in the flowchart flow or flows and/or block diagram block or blocks.
These computer program instructions may also be loaded onto a computer or other programmable data processing apparatus to cause a series of operational steps to be performed on the computer or other programmable apparatus to produce a computer implemented process such that the instructions which execute on the computer or other programmable apparatus provide steps for implementing the functions specified in the flowchart flow or flows and/or block diagram block or blocks.
The foregoing description is only of the preferred embodiments of the present invention, and is not intended to limit the scope of the present invention.
Claims (19)
1. A sample analyzer, comprising:
a dispensing mechanism for dispensing a liquid;
the reaction cup is used for bearing liquid;
the photodetection component is used for detecting liquid in the reaction cup;
a controller for acquiring a liquid parameter of the first liquid, wherein the light intensity decreases after the light of the target wavelength passes through the first liquid; controlling a first dispensing mechanism to inject a diluent into the reaction cup and controlling a second dispensing mechanism to inject the first liquid into the reaction cup; controlling the photodetection part to detect mixed liquid of diluent and first liquid in the reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
2. The sample analyzer of claim 1, wherein the controller is further configured to:
controlling the dispensing mechanism to inject the first liquid into the first reaction cup; and controlling the photodetection component to carry out photodetection on the first liquid in the first reaction cup to obtain the liquid parameter of the first liquid.
3. The sample analyzer according to claim 1 or 2, characterized in that,
the controller is further configured to: controlling the dispensing mechanism to inject the first liquid into the first reaction cup; controlling the photodetection part to carry out photodetection on the first liquid in the first reaction cup to obtain liquid parameters of the first liquid;
the controller is also used for controlling the second dispensing mechanism to absorb the first liquid from the first reaction cup and to inject the first liquid into the second reaction cup and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
4. A sample analyzer as claimed in claim 3, further comprising reagent storage means for storing reagents;
the controller is further configured to: controlling the first dispensing mechanism to draw the first liquid from the reagent storage device and to dispense the first liquid into a first reaction cup; controlling the second dispensing mechanism to draw the first liquid from the first reaction cup and to inject the first liquid into the second reaction cup and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
5. The sample analyzer of claim 1, further comprising: a sample scheduler, the sample scheduler comprising: a storage area for storing samples, and a scheduling mechanism for scheduling samples;
the controller is further configured to: controlling the dispatching mechanism to dispatch the first liquid from the storage area to a liquid sucking position where the second dispensing mechanism sucks the liquid; controlling the second dispensing mechanism to draw the first liquid from the drawing position to be injected into a second reaction cup and controlling the first dispensing mechanism to inject diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
6. The sample analyzer according to claim 1 or 2, characterized in that,
the controller is further configured to: controlling the second dispensing mechanism to suck the first liquid and pour the first liquid into the first reaction cup; controlling the photodetection part to detect the first liquid in the first reaction cup to obtain liquid parameters of the first liquid;
the controller is further configured to: controlling the second dispensing mechanism to draw the first liquid from the first reaction cup and to inject the first liquid into a second reaction cup and controlling the first dispensing mechanism to inject a diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
7. The sample analyzer of claim 1 or 2, wherein the volume of the diluent is greater than the volume of the first liquid;
the controller is further configured to: controlling the first dispensing mechanism to inject a diluent into the reaction cup and controlling the second dispensing mechanism to inject the first liquid into the reaction cup; controlling the photodetection part to detect mixed liquid of diluent and first liquid in the reaction cup, and obtaining a target detection signal under the target wavelength; and determining the sample adding state of the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
8. The sample analyzer of claim 1, wherein the sample analyzer comprises a sample cell,
the controller is further configured to: determining a first absorbance of the first liquid according to the liquid parameter of the first liquid and a second absorbance of the mixed liquid according to the target detection signal;
acquiring the volume of the first liquid injected into the reaction cup by the second dispensing mechanism, and determining the current diluent sample adding volume corresponding to the first dispensing mechanism according to the volume of the first liquid, the first absorbance and the second absorbance; determining a first sample adding error or a first sample adding state of the first dispensing mechanism according to the current diluent sample adding volume and a preset diluent sample adding volume corresponding to the first dispensing mechanism;
and/or the number of the groups of groups,
acquiring the volume of the diluent injected into the reaction cup by the first dispensing mechanism, and determining the current first liquid sample adding volume corresponding to the second dispensing mechanism according to the volume of the diluent, the first absorbance and the second absorbance; and determining a second sample adding error or a second sample adding state of the second dispensing mechanism according to the current first liquid sample adding volume and the preset first liquid sample adding volume corresponding to the second dispensing mechanism.
9. The sample analyzer according to claim 1 or 2, characterized in that,
the controller is further configured to: controlling the photodetection part to continuously detect the first liquid in the first reaction cup to obtain at least two detection signals under the target wavelength; predicting estimated signals corresponding to the target detection period of the first liquid in the sample loading state according to the at least two detection signals;
in a sample adding state target detection period, controlling the second dispensing mechanism to absorb the first liquid from the first reaction cup and inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; determining the sample loading state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the estimated signal;
or,
the controller is further configured to: controlling the photodetection part to detect the first liquid in the first reaction cup to obtain an initial detection signal of the first liquid under the target wavelength; the photodetection part is controlled to continuously detect the first liquid in the first reaction cup, at least two detection signals under the target wavelength are obtained, and a correction coefficient is obtained according to the at least two detection signals;
In a sample adding state target detection period, controlling the second dispensing mechanism to suck the first liquid from the first reaction cup and inject the first liquid into the second reaction cup, and controlling the first dispensing mechanism to inject the diluent into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal, the initial detection signal of the first liquid and the correction coefficient;
or,
the controller is further configured to: in each sample adding state detection period, controlling the photodetection part to detect the first liquid stored in the first reaction cup to obtain the liquid parameter of the first liquid; controlling the first dispensing mechanism to inject diluent into a second reaction cup and controlling the second dispensing mechanism to suck the first liquid from the first reaction cup and inject the first liquid into the second reaction cup; controlling the photodetection part to detect the mixed liquid of the diluent and the first liquid in the second reaction cup, and obtaining a target detection signal under the target wavelength; determining the sample adding state of the first dispensing mechanism or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid; the first liquid stored in the first reaction cup is used for at least two sample adding state detection periods.
10. The sample analyzer of claim 1 or 8, wherein,
the controller is further configured to: when the sample adding state of the first dispensing mechanism or the second dispensing mechanism represents that the sample adding error is larger than a preset error threshold, controlling a built-in or connected prompting device of the sample analyzer to prompt in a preset prompting mode; the preset prompting mode comprises at least one of display screen prompting and acousto-optic prompting;
and/or the number of the groups of groups,
and the controller is also used for correcting the project test result of the sample analyzer according to the sample adding state or the sample adding error.
11. The sample analyzer of any one of claims 1, 2, 4, or 5, further comprising: a stirring rod;
the controller is further configured to: and before the target detection signal of the mixed liquid in the reaction cup is obtained, controlling the stirring rod to stir the liquid in the reaction cup so as to mix the first liquid and the diluent.
12. A sample analyzer, comprising:
a dispensing mechanism for dispensing a liquid;
the reaction cup is used for bearing liquid;
A target device for performing a first operation on the liquid in the reaction cup;
the photodetection component is used for detecting liquid in the reaction cup;
a controller for controlling the dispensing mechanism to inject a first liquid into the reaction cup, wherein the light intensity of the light with the target wavelength is reduced after the light passes through the first liquid; controlling the light detection component to detect the first liquid in the reaction cup to obtain first absorbance at a target wavelength; controlling the target device to perform the first operation on the liquid in the reaction cup; controlling the light detection component to detect the liquid in the reaction cup after the first operation to obtain the second absorbance at the target wavelength; and determining the working state of the target device according to the first absorbance and the second absorbance.
13. The sample analyzer of claim 12, wherein the target device comprises: a puddler, the first operation comprising: the stirring operation is carried out, the stirring device,
the controller is also used for determining the water stagnation state of the stirring rod according to the first absorbance before the stirring operation and the second absorbance after the stirring operation.
14. The sample analyzer of claim 13, wherein the sample analyzer comprises a sample analyzer,
The controller is further configured to: under the condition that the water stagnation state is determined to represent the water stagnation of the stirring rod, controlling a built-in or connected prompting device of the sample analyzer to perform water stagnation prompting in a preset prompting mode; the preset prompting mode comprises at least one of display screen prompting and acousto-optic prompting;
and/or the number of the groups of groups,
the controller is further configured to: under the condition that the water stagnation state represents water stagnation of the stirring rod, starting a preset stirring rod maintenance program in the sample analyzer so as to maintain the stirring rod;
and/or the number of the groups of groups,
the controller is further configured to: determining the water stagnation amount of the stirring rod according to the first absorbance and the second absorbance; and correcting the project test result of the sample analyzer according to the water stagnation state or the water stagnation amount.
15. The sample analyzer of any one of claims 12-14, wherein the target device comprises: a first stirring rod; the first operation includes: first stirring;
the controller is further configured to: in a first project test period, controlling a first dispensing mechanism to inject a reagent into the reaction cup and controlling a second dispensing mechanism to inject a sample into the reaction cup; controlling the first stirring rod to stir the liquid in the reaction cup for the first time; controlling the light detection component, detecting the liquid in the first stirred reaction cup, and obtaining a project test result of the mixed liquid of the sample and the reagent;
The controller is further configured to: controlling the first dispensing mechanism to inject a first liquid into the reaction cup in a first stirring water stagnation detection period, wherein the second dispensing mechanism does not dispense the liquid into the reaction cup; controlling the photodetection part to detect liquid in the reaction cup, and obtaining first absorbance at the target wavelength; controlling the first stirring rod to stir the liquid in the reaction cup for the first time; controlling the photodetection part to detect the liquid in the reaction cup after the first stirring to obtain the second absorbance under the target wavelength; and determining the water stagnation state of the first stirring rod according to the first absorbance and the second absorbance.
16. The sample analyzer of any one of claims 12-14, wherein the target device further comprises: a second stirring rod; the first operation includes: second stirring;
the controller is further configured to: in a second project test period, controlling a first dispensing mechanism to inject a first reagent into the reaction cup and controlling a second dispensing mechanism to inject a sample into the reaction cup; controlling a first stirring rod to stir the liquid in the reaction cup for the first time; controlling a third dispensing mechanism to inject a second reagent into the reaction cup; controlling the second stirring rod to stir the liquid in the reaction cup for the second time; controlling the light measuring component, and detecting liquid in the reaction cup to obtain a project test result;
The controller is further configured to: controlling the first dispensing mechanism or the second dispensing mechanism to inject the first liquid into the reaction cup in a second stirring water stagnation detection period; controlling the first stirring rod to stir the liquid in the reaction cup for the first time; controlling the light detection component to detect liquid in the first stirred reaction cup, and obtaining first absorbance at the target wavelength; the third dispensing mechanism does not dispense, and controls the second stirring rod to stir the liquid in the reaction cup for the second time; controlling the light detection component to detect liquid in the second stirred reaction cup, and obtaining second absorbance at the target wavelength; and determining the water stagnation state of the second stirring rod according to the first absorbance and the second absorbance.
17. The sample analyzer of any of claims 12-14, wherein,
the first operation includes the target dispensing mechanism injecting a diluent into the cuvette,
the controller is further configured to: and determining the sample adding state of the target dispensing mechanism according to the absorbance of the first liquid in the reaction cup and the absorbance of the mixed liquid containing the first liquid and the diluent in the reaction cup.
18. A control method of a sample analyzer, the control method comprising:
acquiring a liquid parameter of the first liquid, wherein the light intensity is reduced after the light with the target wavelength passes through the first liquid;
controlling a first dispensing mechanism to inject diluent into a reaction cup and controlling a second dispensing mechanism to inject first liquid into the reaction cup;
controlling a photodetection part to acquire a target detection signal of a mixed solution of the diluent and the first liquid in the reaction cup under the target wavelength;
and determining the sample adding state of the first dispensing mechanism and/or the second dispensing mechanism according to the target detection signal and the liquid parameter of the first liquid.
19. A control method of a sample analyzer, the control method comprising:
controlling a dispensing mechanism to inject a first liquid into the reaction cup, wherein the light intensity of the light with the target wavelength is reduced after the light with the target wavelength passes through the first liquid;
controlling a photodetection part to detect first liquid in the reaction cup and obtain first absorbance at a target wavelength;
controlling the target device to perform a first operation on the liquid in the reaction cup;
controlling the light detection component, detecting the first liquid after the first operation, and obtaining second absorbance at the target wavelength;
And determining the working state of the target device according to the first absorbance and the second absorbance.
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| CN202211216872 | 2022-09-30 | ||
| CN2022112168722 | 2022-09-30 |
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| CN202211365728.5A Pending CN117805424A (en) | 2022-09-30 | 2022-10-31 | Sample analyzer and control method for sample analyzer |
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