CN117801928B - Constant temperature incubator convenient to store - Google Patents
Constant temperature incubator convenient to store Download PDFInfo
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- CN117801928B CN117801928B CN202410210730.8A CN202410210730A CN117801928B CN 117801928 B CN117801928 B CN 117801928B CN 202410210730 A CN202410210730 A CN 202410210730A CN 117801928 B CN117801928 B CN 117801928B
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- 235000015097 nutrients Nutrition 0.000 claims abstract description 61
- 238000001802 infusion Methods 0.000 claims abstract description 27
- 230000007246 mechanism Effects 0.000 claims abstract description 18
- 238000004140 cleaning Methods 0.000 claims description 17
- 238000004659 sterilization and disinfection Methods 0.000 claims description 14
- 230000001105 regulatory effect Effects 0.000 claims description 13
- 239000012528 membrane Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 9
- 230000001276 controlling effect Effects 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 4
- 238000004506 ultrasonic cleaning Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 abstract description 3
- 241000973497 Siphonognathus argyrophanes Species 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 21
- 230000006872 improvement Effects 0.000 description 9
- 230000008569 process Effects 0.000 description 8
- 238000003860 storage Methods 0.000 description 4
- 238000004544 sputter deposition Methods 0.000 description 3
- 206010011409 Cross infection Diseases 0.000 description 2
- 206010029803 Nosocomial infection Diseases 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 230000009471 action Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 210000002421 cell wall Anatomy 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 239000000645 desinfectant Substances 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003204 osmotic effect Effects 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/26—Conditioning fluids entering or exiting the reaction vessel
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
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- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Sustainable Development (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Thermal Sciences (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention relates to the field of microorganism culture, in particular to a constant temperature incubator convenient to store. Technical problems: the nutrient solution is added to the culture bottles one by one manually, so that the operation is complicated, the culture box is required to be opened continuously, the culture bottles are taken out, the pollution and the influence of external mixed bacteria are easy to occur, and the culture is not facilitated. The technical scheme is as follows: a constant temperature incubator convenient to store comprises a incubator body, an incubator door, a driving shell, a placing plate and the like; the box body is fixedly connected with a box door; the box door is provided with an observation window for observing the inside of the box body; the box body is fixedly connected with a driving shell; a plurality of placing plates are fixedly connected in the box body. The tube mouth of the infusion hose is driven to move to the position right above the culture tube, then the electric push rod is controlled to drive the second cylinder, the infusion hose and other connected parts to move downwards, so that the infusion hose moves downwards into the culture tube, and then the nutrient solution conveying mechanism is controlled to input the required nutrient solution into the infusion hose, so that the nutrient solution is added into the culture tube.
Description
Technical Field
The invention relates to the field of microorganism culture, in particular to a constant temperature incubator convenient to store.
Background
In the cell culture operation, an incubator is adopted, cells are placed in an incubator, nutrient solution is added into the incubator, and then the incubator is placed in the incubator, and the temperature is set to be proper, so that the cells are cultured;
In the actual use process, in order to ensure normal growth and division of cells, more nutrient solution needs to be added into a culture bottle, but the cell quantity in an initial state is small, and the cell wall is damaged by more nutrient solution due to the osmotic pressure problem, so that in the cultivation process, a small amount of nutrient solution is firstly added, then the culture bottle is periodically added again, and the nutrient solution is manually added into a culture tube one by one, so that the operation is complicated, the culture box needs to be continuously opened, the culture bottle is taken out, and pollution and influence of external mixed bacteria are easy to occur, and the cultivation is not facilitated; and people can sometimes cover the culture flask and then put the culture flask into the culture flask when putting the culture flask into the culture flask, and can sometimes not cover and directly put the culture flask, but when adding the nutrient solution, the nutrient solution can not be added to the culture flask with the cover in the conventional culture flask.
Disclosure of Invention
In order to overcome the defects that nutrient solution is added to the culture bottles one by one manually, the operation is complicated, the culture box is required to be opened continuously, the culture bottles are taken out, the pollution and the influence of external mixed bacteria are easy to occur, and the culture is not facilitated, the invention provides the constant temperature culture box convenient to store.
The technical scheme is as follows: a constant temperature incubator convenient to store comprises a incubator body, an incubator door, a driving shell, a placing plate and a bearing plate; the box body is fixedly connected with a box door; the box door is provided with an observation window for observing the inside of the box body; the box body is fixedly connected with a driving shell; a plurality of placing plates are fixedly connected in the box body, and a plurality of placing holes are formed in the placing plates; a plurality of supporting plates are fixedly connected in the box body, the supporting plates are opposite to the placing plates, the supporting plates are provided with placing grooves, and the placing grooves are opposite to the corresponding placing holes; the device also comprises a power unit, a driving unit, a first cylinder, an electric push rod, a second cylinder, a sucker, a limiting ring, an infusion hose, an inclined plate, a slip ring, a pressure sensor and a constant temperature component; the bearing plate is hollow, and a pressure sensor is arranged below each placing groove; the driving shell is connected with a power unit; the power unit is connected with the first cylinder and is used for driving the first cylinder to move; the first cylinder is fixedly connected with a plurality of electric push rods; all the telescopic parts of the electric push rod are fixedly connected with a cylinder II, and the cylinder II is in sliding connection with the cylinder I; the second cylinder is connected with a driving unit; the driving unit is connected with a sucker and is used for driving the sucker to move; the sucker is fixedly connected with a limiting ring; the second cylinder is provided with a plurality of infusion hoses; the second cylinder is rotationally connected with a plurality of sloping plates through torsion springs; each inclined plate is fixedly connected with a plurality of sliding rings, the transfusion hose is in sliding connection with the sliding rings, and the transfusion hose is communicated with the nutrient solution conveying mechanism; the box body is connected with a constant temperature component for controlling temperature.
As an improvement of the above-mentioned scheme, the swash plate is provided with an arc portion.
As an improvement of the scheme, the device also comprises a servo motor, a screw rod, a movable plate and an elastic membrane; the box body is fixedly connected with a servo motor; the box body is rotationally connected with a screw rod, and the screw rod is fixedly connected with an output shaft of the servo motor; the screw rod is connected with a movable plate in a sliding way; the movable plate is fixedly connected with an elastic membrane, and the elastic membrane is fixedly connected with the inner wall of the box body.
As an improvement of the scheme, the ultraviolet lamp also comprises a wafer and an ultraviolet lamp; two circular sheets are fixedly connected at the pipe orifice of each transfusion hose respectively; the inner wall of the cylinder II is respectively provided with a plurality of ultraviolet lamps for sterilization and disinfection.
As an improvement of the scheme, the device also comprises a connecting rod, a fixed ring and a lantern ring; the limiting ring is fixedly connected with a plurality of connecting rods; all the connecting rods are fixedly connected with a fixed ring; the fixed ring is fixedly connected with a lantern ring, the fixed ring is positioned at the outer side part of the lantern ring, and the lantern ring is made of flexible materials; the bottom of the lantern ring is provided with a groove.
As an improvement of the scheme, the device also comprises an elastic telescopic rod, a trapezoid block, a film and a clamping plate; a plurality of elastic telescopic rods are fixedly connected at the placement holes of each placement plate respectively; each elastic telescopic rod is fixedly connected with a trapezoid block; each trapezoid block is fixedly connected with a clamping plate; the placing holes of each placing plate are fixedly connected with a plurality of films respectively, the films are fixedly connected with the clamping plates, and the films wrap the elastic telescopic rods and the trapezoid blocks.
As an improvement of the scheme, the device also comprises a placing platform, a cleaning box, a sterilizing box and a sponge; the box body is fixedly connected with a placement platform; the placing platform is provided with a cleaning box; the cleaning box is provided with a sponge; the placing platform is provided with a disinfection box, disinfection liquid is added into the disinfection box, and an ultrasonic cleaning mechanism is arranged in the disinfection box.
As an improvement of the above-mentioned solution, the sponge is provided with protrusions.
As an improvement of the scheme, the constant temperature component comprises a temperature regulating box, an air inlet pipe, an air outlet pipe and a temperature detector; the box body is fixedly connected with a temperature regulating box, and a hot air mechanism and a cold air mechanism for controlling the temperature are arranged in the temperature regulating box; the temperature regulating box is communicated with an air inlet pipe which is communicated with the box body; the temperature adjusting box is communicated with an air outlet pipe which is communicated with the box body; a temperature detector is arranged in the box body.
As an improvement of the scheme, the device also comprises a first connecting pipe, a diaphragm and a second connecting pipe; the box body is fixedly connected with a first connecting pipe which is communicated with the air inlet pipe, and the first connecting pipe is communicated with the inside of the bearing plate; the box body is fixedly connected with a second connecting pipe which is communicated with the air outlet pipe, and the second connecting pipe is communicated with the inside of the bearing plate; each placing groove is provided with a diaphragm, and the diaphragms are positioned above the pressure sensors.
The beneficial effects are that:
the tube mouth of the infusion hose is driven to move to the position right above the culture tube, then the electric push rod is controlled to drive the second cylinder, the infusion hose and other connected parts to move downwards, so that the infusion hose moves downwards into the culture tube, and then the nutrient solution conveying mechanism is controlled to input the required nutrient solution into the infusion hose, so that the nutrient solution is added into the culture tube.
Through the curved arc portion that the swash plate set up, drive swash plate and infusion hose downwardly moving at electric putter, when infusion hose got into and cultivates the test tube, the mouth of pipe of infusion hose is under the restriction of curved arc portion, be curved arc form, when making infusion hose get into and cultivate the test tube, the mouth of pipe of curved arc form's infusion hose can be close to with the wall of cultivateing the test tube, make follow-up when infusion hose adds the nutrient solution, can add the wall department at cultivateing the test tube with the nutrient solution, make the nutrient solution of adding flow into the cell sap of below downwards along cultivateing the test tube wall, avoid direct interpolation can cause the sputtering of cultivateing the test tube interior cell sap, influence the normal cultivation of cell.
The second electric telescopic rod is controlled to drive the sucker and other connected parts to move downwards, when the sucker contacts with the plug, the sucker is stopped to adsorb the plug, and then the second electric telescopic rod is controlled to drive the sucker and the plug to move upwards, so that the plug is pulled out conveniently.
Drawings
FIG. 1 is a schematic diagram of a disclosed structure of a conveniently stored incubator of the present invention;
FIG. 2 is a cross-sectional view of a convenient storage incubator of the present invention;
FIG. 3 is a view showing the state of a first cylinder, an electric push rod and a second cylinder of the incubator disclosed by the invention, which is convenient to store;
FIG. 4 is a schematic view showing the combined structure of the sucking disc, the limiting ring, the transfusion hose and the inclined plate disclosed by the constant temperature incubator convenient to store;
fig. 5 is an enlarged view at a in fig. 4;
FIG. 6 is a schematic view showing the combined structure of the connecting rod, the fixing ring and the collar disclosed by the incubator convenient to store;
FIG. 7 is a schematic view showing the combined structure of an elastic telescopic rod, a trapezoid block, a film and a clamping plate disclosed by the constant temperature incubator convenient to store;
FIG. 8 is a schematic view showing the combined structure of a placement platform, a cleaning box and a sponge disclosed in the incubator of the present invention which is convenient to store;
FIG. 9 is a schematic view showing the structure of a thermostatic element disclosed in a thermostatic incubator for easy storage of the present invention;
FIG. 10 is a schematic view showing a combined structure of a connection pipe I, a diaphragm and a pressure sensor disclosed in the incubator of the present invention which is convenient to store.
Reference numerals in the figures: 1-box, 2-box door, 3-drive housing, 4-placing plate, 5-supporting plate, 6-cylinder one, 7-electric push rod, 8-cylinder two, 9-suction cup, 10-limit ring, 11-infusion hose, 12-inclined plate, 13-slip ring, 14-ultraviolet lamp, 101-electric guide rail one, 102-moving block one, 103-electric guide rail two, 104-moving block two, 105-electric telescopic rod one, 106-rotating rod, 107-gear one, 108-driving motor, 109-gear two, 1010-electric telescopic rod two, 111-servo motor, 112-screw rod, 113-moving plate, 114-elastic membrane, 121-circular disc, 131-connecting rod, 132-fixed ring, 133-collar, 141-elastic telescopic rod, 142-trapezoidal block, 143-film, 144-clamping plate, 201-placing platform, 202-cleaning box, 203-sponge, 204-sterilizing box, 301-temperature adjusting box, 302-air inlet pipe, 303-air outlet pipe, 304-temperature detector, 311-one, 312-diaphragm, 313-pressure sensor, 314-2-observation window, 2-2 a, 2-moving plate, 121-circular disc, 131-connecting tube, 133-fixed ring, 133-groove, 100a, arc-groove, and 100-groove.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
A constant temperature incubator convenient to store, as shown in figures 1-7 and 10, comprises a incubator body 1, an incubator door 2, a drive casing 3, a placing plate 4 and a bearing plate 5; the box body 1 is fixedly connected with a box door 2; the box door 2 is provided with an observation window 2a; the box body 1 is fixedly connected with a driving shell 3; a plurality of placing plates 4 are fixedly connected in the box body 1, and a plurality of placing holes are formed in the placing plates 4; a plurality of supporting plates 5 are fixedly connected in the box body 1, the supporting plates 5 are opposite to the placing plates 4, the supporting plates 5 are provided with placing grooves 5a, and the placing grooves 5a are opposite to the corresponding placing holes;
the device also comprises a power unit, a driving unit, a first cylinder 6, an electric push rod 7, a second cylinder 8, a sucker 9, a limiting ring 10, an infusion hose 11, an inclined plate 12, a slip ring 13, a pressure sensor 313 and a constant temperature component; the bearing plate 5 is hollow, and a pressure sensor 313 is arranged below each placing groove 5 a; the driving shell 3 is connected with a power unit; the power unit is connected with the first cylinder 6 and is used for driving the first cylinder 6 to move; the first cylinder 6 is fixedly connected with two electric push rods 7; the telescopic parts of all the electric push rods 7 are fixedly connected with a cylinder II 8, and the cylinder II 8 is in sliding connection with the cylinder I6; the second cylinder 8 is connected with a driving unit; the driving unit is connected with a sucker 9 and is used for driving the sucker 9 to move; the sucker 9 is fixedly connected with a limiting ring 10; the second cylinder 8 is provided with two infusion hoses 11; the second cylinder 8 is rotationally connected with two sloping plates 12 through a torsion spring; each inclined plate 12 is fixedly connected with a plurality of sliding rings 13, the transfusion hose 11 is in sliding connection with the sliding rings 13, and the transfusion hose 11 is communicated with the nutrient solution conveying mechanism; the box 1 is connected with a constant temperature component.
The inclined plate 12 is provided with an arc part 12a, and can guide the transfusion hose 11 to be arc-shaped, so that when the transfusion hose is used for conveying nutrient solution, the nutrient solution can be conveyed to the wall surface of the culture test tube 100, so that the nutrient solution is added from the wall surface to avoid the problem of sputtering.
The device also comprises a servo motor 111, a screw rod 112, a movable plate 113 and an elastic membrane 114; the box body 1 is fixedly connected with a servo motor 111; the box body 1 is rotationally connected with a screw rod 112, and the screw rod 112 is fixedly connected with an output shaft of a servo motor 111; the screw rod 112 is connected with a movable plate 113 in a sliding way; the movable plate 113 is fixedly connected with an elastic membrane 114, and the elastic membrane 114 is fixedly connected with the inner wall of the box body 1.
Also included are a wafer 121 and an ultraviolet lamp 14; two circular discs 121 are fixedly connected to the pipe orifice of each transfusion hose 11, and the circular discs 121 are made of flexible materials; a plurality of ultraviolet lamps 14 are respectively arranged on the inner wall of the cylinder II 8.
Also comprises a connecting rod 131, a fixed ring 132 and a lantern ring 133; the limiting ring 10 is fixedly connected with a plurality of connecting rods 131; all the connecting rods 131 are fixedly connected with a fixed ring 132; the fixing ring 132 is fixedly connected with a collar 133, the fixing ring 132 is positioned at the outer side part of the collar 133, and the collar 133 is made of flexible materials; the bottom of the collar 133 is provided with a groove 133a.
Also comprises an elastic telescopic rod 141, a trapezoid block 142, a film 143 and a clamping plate 144; two elastic telescopic rods 141 are fixedly connected at the placement holes of each placement plate 4 respectively; each elastic telescopic rod 141 is fixedly connected with a trapezoid block 142; each trapezoid block 142 is fixedly connected with a clamping plate 144; the placing holes of each placing plate 4 are fixedly connected with a plurality of films 143, the films 143 are fixedly connected with clamping plates 144, and the films 143 wrap the elastic telescopic rods 141 and the trapezoid blocks 142.
The power unit comprises a first electric guide rail 101, a first moving block 102, a second electric guide rail 103, a second moving block 104 and a first electric telescopic rod 105; the driving shell 3 is fixedly connected with two electric guide rails 101; each electric guide rail I101 is connected with a moving block I102 in a sliding way; the two first moving blocks 102 are fixedly connected with an electric guide rail II 103; the second electric guide rail 103 is connected with a second moving block 104 in a sliding manner; the second moving block 104 is fixedly connected with two first electric telescopic rods 105, and all telescopic parts of the first electric telescopic rods 105 are fixedly connected with the first cylinder 6.
The driving unit comprises a rotating rod 106, a first gear 107, a driving motor 108, a second gear 109 and a second electric telescopic rod 1010; the second cylinder 8 is rotatably connected with a rotating rod 106; the rotating rod 106 is fixedly connected with a first gear 107; the second cylinder 8 is fixedly connected with a driving motor 108; the output shaft of the driving motor 108 is fixedly connected with a gear II 109, and the gear II 109 is meshed with a gear I107; the rotating rod 106 is fixedly connected with two electric telescopic rods II 1010, and telescopic parts of all the electric telescopic rods II 1010 are fixedly connected with the sucker 9.
The working steps of the embodiment are as follows: when the device specifically works, corresponding electrical parts are communicated with an external power supply by manpower, then a culture test tube 100 filled with cultured cells is manually placed in a box body 1 and passes through a placement hole of a placement plate 4 to be placed on a placement groove 5a of a bearing plate 5, as shown in fig. 2, after a plurality of culture test tubes 100 are placed in the box body 1, a box door 2 is closed, the box body 1 is sealed, and then a constant temperature component is controlled to regulate and control the temperature of the box body 1, so that the temperature in the box body 1 is suitable for the growth of the cells in the culture test tube 100, and the normal cultivation of the cells in the culture test tube 100 is ensured;
As the cells in the culture tube 100 proliferate, the nutrient solution in the culture tube is gradually reduced, the overall weight of the culture tube 100 changes, when the nutrient solution is required to be added into the culture tube, the servo motor 111 is controlled to drive the screw rod 112 to rotate, the screw rod 112 rotates to drive the movable plate 113 to move, and the movable plate 113 moves to shrink and retract the elastic membrane 114; then the first moving block 102 is controlled to drive the second electric guide rail 103 and the second moving block 104 and other connected parts to move back and forth along the first electric guide rail 101, the second moving block 104 is controlled to drive the first electric telescopic rod 105 and the first cylinder 6 and other connected parts to move left and right along the second electric guide rail 103, the first electric telescopic rod 105 and the first cylinder 6 and other connected parts are driven to move right above the culture tube 100 needing nutrient solution addition through the mutual matching of the first moving block 102 and the second moving block 104, the first electric telescopic rod 105 is controlled to drive the first cylinder 6 and other connected parts to move downwards, the movement is stopped when the first cylinder 6 comes into contact with the placement plate 4, and the first cylinder 6 and the second cylinder 8 cover the culture tube 100 as shown in FIG. 3; then, the nutrient solution adding operation is started, in the process of cultivating the cultivating test tube 100 in the box body 1, in order to reduce the loss of water in the cultivating test tube 100, people can cover the cultivating test tube 100 through the plug 200 during cultivation, so as to avoid the water in the cultivating test tube from flowing out and affecting the cultivation of internal cells during cultivation, therefore, before the nutrient solution is added, the second electric telescopic rod 1010 is controlled to drive the sucker 9 and other connected parts to move downwards, when the sucker 9 contacts with the plug 200, the plug 200 is adsorbed through the sucker 9, then the second electric telescopic rod 1010 is controlled to drive the sucker 9 and the plug 200 to move upwards, the plug 200 is pulled out of the culture tube 100, and in the process of pulling out the plug 200, the driving motor 108 can be controlled to drive the gear I107 to rotate, the gear I107 drives the gear II 109, the rotating rod 106, the electric telescopic rod II 1010 and the sucker 9 to rotate, and the forward rotation and the reverse rotation are repeated 180 degrees, and the sucker 9 is matched with the repeated rotation, so that the plug 200 is pulled out by the sucker 9; it should be noted that when the first cylinder 6 moves downward to contact with the placement plate 4, the first cylinder 6 will squeeze the film 143 and the trapezoid block 142 to drive the trapezoid block 142 and the clamp plate 144 to move, the elastic telescopic rod 141 is stretched, and the clamp plates 144 move mutually, so that when the first cylinder 6 covers the culture tube 100, the clamp plates 144 can move to clamp and fix the culture tube 100, the plug 200 can be normally pulled out, and the elastic telescopic rod 141 and the trapezoid block 142 can be always wrapped by the film 143, so that the inside of the culture tube is prevented from being easily polluted during use; after the plug 200 is pulled out, the culture tube 100 can be isolated through the masks of the first cylinder 6 and the second cylinder 8, so that the gas in the culture bottle can be directly dissipated into the box body 1 to pollute the box body 1 in the process of adding nutrient solution by opening the cover later;
As described herein, the culture tube 100 is capped to culture different cells, and the culture tube 100 is not capped to culture the same cells;
Meanwhile, when the culture tube 100 is placed in the placement groove 5a, the pressure sensor 313 arranged below the placement groove 5a is in contact with the culture tube 100, and when the whole weight of the culture tube 100 is changed, the pressure sensor 313 can be used for detecting to judge whether the culture tube 100 needs to be added with nutrient solution or not;
The transfusion hose 11 is arranged on the inclined plate 12, when the first cylinder 6 and the second cylinder 8 downwards move to shade the culture test tube 100, the inclined plate 12 is extruded by the limiting ring 10, so that the inclined plate 12 drives the transfusion hose 11 to rotate and be attached to the wall surface of the second cylinder 8, and the inclined plate 12 and the transfusion hose 11 can prevent the first cylinder 6 and the second cylinder 8 from downwards moving in the process of downwards moving the first cylinder 6 and the second cylinder 8; meanwhile, when the second electric telescopic rod 1010 drives the suction cup 9 and the plug 200 to move upwards and pull out, the suction cup 9 is continuously driven to move upwards, the suction cup 9 drives the limiting ring 10 to move upwards, the inclined plate 12 gradually loses extrusion along with the upward movement of the limiting ring 10, the inclined plate 12 drives the transfusion hose 11 to rotate and reset under the action of the torsion spring, so that the two inclined plates 12 rotate and reset to be in a reverse splayed shape, the pipe orifice of the transfusion hose 11 is driven to move right above the culture test tube 100, then the electric push rod 7 is controlled to drive the second cylinder 8 and the transfusion hose 11 and other connected parts to move downwards, so that the transfusion hose 11 moves downwards into the culture test tube 100, then the nutrient solution conveying mechanism is controlled to input the required nutrient solution into the transfusion hose 11, and the nutrient solution is added into the culture test tube 100 through the transfusion hose 11, so that the nutrient solution can be added into the capped culture bottle;
However, the nutrient solution is directly added into the culture tube 100 from top to bottom through the transfusion hose 11, and the nutrient solution drops into the culture tube 100 from top to bottom, so that the cell solution in the culture tube 100 is easily sputtered, and part of cells are sputtered onto the wall surface of the culture tube 100, so that the part of cells cannot be contacted with the nutrient solution in the culture tube 100, and the cells die; therefore, when the nutrient solution is added, through the curved arc part 12a arranged on the inclined plate 12, when the electric push rod 7 drives the inclined plate 12 and the transfusion hose 11 to move downwards, and the transfusion hose 11 enters the culture test tube 100, the pipe orifice of the transfusion hose 11 is in a curved arc shape under the limitation of the curved arc part 12a, so that when the transfusion hose 11 enters the culture test tube 100, the pipe orifice of the curved arc transfusion hose 11 can be close to the wall surface of the culture test tube 100, and when the nutrient solution is added into the transfusion hose 11, the nutrient solution can be added at the wall surface of the culture test tube 100, so that the added nutrient solution flows downwards into the cell solution below along the wall surface of the culture test tube 100, and the direct addition is avoided to cause the sputtering of the cell solution in the culture test tube 100, thereby affecting the normal culture of cells; the circular sheet 121 is arranged at the pipe orifice of the infusion hose 11, so that the problem that the pipe orifice of the infusion hose 11 is blocked and the conveying of nutrient solution is affected due to the fact that the pipe orifice of the infusion hose 11 is directly attached to the wall surface of the culture test tube 100 can be avoided;
after the nutrient solution is added, the electric push rod 7 is controlled to drive the second cylinder 8 and other connected parts to move upwards, the transfusion hose 11 is moved out of the culture tube 100, the electric telescopic rod 1010 is subsequently controlled to drive the sucker 9 and the plug 200 to move downwards, the plug 200 is inserted back into the culture tube 100, and the addition of the nutrient solution of the culture tube 100 is completed; however, in the process of moving the infusion hose 11 out of the culture tube 100, the nutrient solution is easy to drive to move, so that the nutrient solution is attached near the tube orifice of the culture tube 100, and when the subsequent plug 200 is inserted back, the nutrient solution is easy to remain, so that after the infusion hose 11 is moved out of the culture tube 100, the second electric telescopic rod 1010 is controlled to drive the sucker 9 and other connected parts to move downwards; the second electric telescopic rod 1010 drives the connecting rod 131, the fixed ring 132 and the lantern ring 133 to move downwards through the limiting ring 10, when the groove 133a of the lantern ring 133 is sleeved on the bottle mouth of the culture test tube 100, the movement is stopped, then the driving motor 108 is controlled to drive the second electric telescopic rod 1010, the lantern ring 133 and other connected parts to rotate, the second electric telescopic rod and the lantern ring 133 rotate 180 degrees in the forward direction and the reverse direction repeatedly, the bottle mouth of the culture test tube 100 is cleaned by driving the lantern ring 133 to repeatedly rotate, residual nutrient solution is cleaned and removed, and in this description, the nutrient solution amount attached near the mouth of the culture test tube 100 is small; after cleaning, the second electric telescopic rod 1010 is continuously controlled to drive the sucker 9 and the plug 200 to move downwards so as to insert the plug 200 back, and in the process, the second electric telescopic rod 1010 drives the connecting rod 131, the fixing ring 132 and the lantern ring 133 to move downwards continuously, and the lantern ring 133 can be deformed and sleeved on the outer wall of the culture test tube 100 so as to ensure the plug 200 to be inserted back; meanwhile, when the lantern ring 133 deforms, the inner side part deforms upwards, at the moment, part of residual nutrient solution at the groove 133a contacts with the outer wall of the culture tube 100, and when the lantern ring 133 is controlled to move upwards and reset subsequently, the residual nutrient solution at the outer wall of the culture tube 100 can be continuously wiped through the lantern ring 133, and at the moment, the residual nutrient solution exists at the groove 133a and the inner side surface of the lantern ring 133;
After the nutrient solution is added into one culture tube 100, the ultraviolet lamp 14 is controlled to irradiate the first cylinder 6 and the second cylinder 8, so that the interior of the first cylinder 6 and the interior of the second cylinder 8 are disinfected and sterilized, and cross infection caused by the subsequent addition of the nutrient solution into other culture tubes 100 is avoided; then the corresponding parts are controlled to drive the first cylinder 6 and other connected parts to move upwards for resetting, then the servo motor 111 is controlled to drive the screw rod 112 to rotate, the movable plate 113 is driven to move to stretch and spread the elastic membrane 114, the first cylinder 6 and other parts are isolated from the storage space of the box body 1, and the storage space in the box body 1 can be reduced, so that the temperature inside the box body 1 can be increased or reduced more rapidly during the subsequent temperature regulation and control of the box body 1.
Example 2
On the basis of the embodiment 1, as shown in fig. 8, the device further comprises a placing platform 201, a cleaning box 202, a sterilizing box 204 and a sponge 203; the box body 1 is fixedly connected with a placing platform 201; the placement platform 201 is provided with a cleaning box 202; the cleaning box 202 is provided with a sponge 203; the placing platform 201 is provided with a disinfection box 204, disinfection liquid is added inside the disinfection box 204, and an ultrasonic cleaning mechanism is arranged inside the disinfection box 204.
The sponge 203 is provided with protrusions 203a, by means of which protrusions 203a the groove 133a of the collar 133 can be cleaned.
On the basis of the above embodiment, after the addition of the nutrient solution of one culture tube 100 is completed through the first cylinder 6 and other connected parts, residual nutrient solution is attached to the collar 133 for cleaning, and when the nutrient solution is added to other culture tubes 100 later, the residual nutrient solution is easy to pollute, so after the addition of the nutrient solution of one culture tube 100 is completed, the first cylinder 6 and other connected parts are driven to move onto the placing platform 201 by controlling the mutual matching of the first moving block 102, the second moving block 104 and the first electric telescopic rod 105, the first cylinder 6 and other connected parts are firstly moved above the sterilizing box 204, then the electric push rod 7 and the second electric telescopic rod 1010 are controlled to mutually match, the second cylinder 8 and the collar 133 are driven to move downwards, the collar 133 is driven to move downwards into the sterilizing box 204, and then an ultrasonic cleaning mechanism in the sterilizing box 204 is controlled to clean and sterilize the collar 133, and the residual nutrient solution at the groove 133a and the inner side of the collar 133; after the cleaning, the first moving block 102, the second moving block 104 and the first electric telescopic rod 105 are controlled to be matched with each other, the first cylinder 6 and other connected parts are driven to move to the upper part of the cleaning box 202, the second electric push rod 7 and the second electric telescopic rod 1010 are controlled to be matched with each other, the second cylinder 8 and the lantern ring 133 are driven to continuously move downwards, the lantern ring 133 is contacted with the sponge 203, the lantern ring 133 is cleaned, residual disinfectant is cleaned, the groove 133a can be embedded with the protrusion 203a, cleaning of the lantern ring 133 is completed, and the lantern ring 133 can be repeatedly used; after cleaning, the ultraviolet lamp 14 is controlled to radiate ultraviolet rays, so that the interior of the first cylinder 6 and the interior of the second cylinder 8 are thoroughly disinfected and sterilized, and the condition that cross infection can not occur during subsequent use is ensured.
Example 3
On the basis of embodiment 2, as shown in fig. 2 and 9-10, the constant temperature component comprises a temperature adjusting box 301, an air inlet pipe 302, an air outlet pipe 303 and a temperature detector 304; the box body 1 is fixedly connected with a temperature regulating box 301, and a hot air mechanism and a cold air mechanism for controlling temperature are arranged in the temperature regulating box 301; the temperature regulating box 301 is communicated with an air inlet pipe 302, and the air inlet pipe 302 is communicated with the box body 1; the temperature adjusting box 301 is communicated with an air outlet pipe 303, and the air outlet pipe 303 is communicated with the box body 1; a temperature detector 304 is provided inside the case 1.
The device also comprises a first connecting pipe 311, a diaphragm 312 and a second connecting pipe 314; the box body 1 is fixedly connected with a first connecting pipe 311, the first connecting pipe 311 is communicated with the air inlet pipe 302, and the first connecting pipe 311 is communicated with the inside of the bearing plate 5; the box body 1 is fixedly connected with a second connecting pipe 314, the second connecting pipe 314 is communicated with the air outlet pipe 303, and the second connecting pipe 314 is communicated with the inside of the bearing plate 5; one diaphragm 312 is provided at each placement groove 5a, and the diaphragm 312 is located above the pressure sensor 313. On the basis of the embodiment, when the culture tube 100 is placed in the box body 1 for cultivation, corresponding hot air or cold air is introduced into the box body 1 through the air inlet pipe 302 by controlling the hot air mechanism and the cold air mechanism in the temperature regulating box 301, and is discharged from the air outlet pipe 303, and the temperature inside the box body 1 is regulated and controlled by matching with the detection of the temperature detector 304, so that the inside of the box body 1 is in a constant temperature state and is suitable for the growth of cells in the culture tube 100;
However, the support plate 5 is provided with a placement groove 5a which is convenient to store, when the culture tube 100 is placed on the placement groove 5a, the bottom of the culture tube 100 is more difficult to contact with the air in the box body 1 than other parts, so that the bottom of the culture tube 100 is heated unevenly, and further the cultivation of cells in the culture tube 100 is affected; through the diaphragm 312 arranged on the support plate 5, when the temperature-adjusting gas is introduced into the air inlet pipe 302, part of the temperature-adjusting gas is introduced into the support plate 5 through the first connecting pipe 311 and is introduced into the air outlet pipe 303 again through the second connecting pipe 314, so that when the temperature-adjusting box 301 is ventilated and adjusted, part of the gas can be introduced into the support plate 5, and the bottom of the culture test tube 100 is heated through the diaphragm 312, so that the whole culture test tube 100 is heated uniformly;
And be provided with diaphragm 312 in standing groove 5a department for when placing culture tube 100, diaphragm 312 can cushion culture tube 100, makes the manual work when depositing culture tube 100, and is more convenient, avoids the manual work to pass the hole of placing board 4, when placing culture tube 100, the bottom of culture tube 100 can be direct with standing groove 5a striking contact, leads to the breakage of culture tube 100 easily.
While the present invention has been described in detail with reference to the above embodiments, it will be apparent to those skilled in the art from this disclosure that various changes or modifications can be made therein without departing from the spirit and scope of the invention as defined in the following claims. Accordingly, the detailed description of the disclosed embodiments is to be taken only by way of illustration and not by way of limitation, and the scope of protection is defined by the content of the claims.
Claims (6)
1. A constant temperature incubator convenient to store comprises a box body (1), a box door (2), a driving shell (3), a placing plate (4) and a bearing plate (5); the box body (1) is fixedly connected with a box door (2); the box door (2) is provided with an observation window (2 a) for observing the inside of the box body (1); the box body (1) is fixedly connected with a driving shell (3); a plurality of placing plates (4) are fixedly connected in the box body (1), and a plurality of placing holes are formed in the placing plates (4); a plurality of supporting plates (5) are fixedly connected in the box body (1), the supporting plates (5) are opposite to the placing plates (4), the supporting plates (5) are provided with placing grooves (5 a), and the placing grooves (5 a) are opposite to the corresponding placing holes; the method is characterized in that: the infusion device also comprises a power unit, a driving unit, a first cylinder (6), an electric push rod (7), a second cylinder (8), a sucker (9), a limiting ring (10), an infusion hose (11), an inclined plate (12), a slip ring (13), a pressure sensor (313) and a constant temperature component; the bearing plate (5) is hollow, and a pressure sensor (313) is arranged below each placing groove (5 a); the driving shell (3) is connected with a power unit; the power unit is connected with the first cylinder (6) and is used for driving the first cylinder (6) to move; the first cylinder (6) is fixedly connected with a plurality of electric push rods (7); the telescopic parts of all the electric push rods (7) are fixedly connected with a cylinder II (8), and the cylinder II (8) is in sliding connection with the cylinder I (6); the second cylinder (8) is connected with a driving unit; the driving unit is connected with a sucker (9) and is used for driving the sucker (9) to move; the sucker (9) is fixedly connected with a limiting ring (10); the cylinder II (8) is provided with a plurality of infusion hoses (11); the cylinder II (8) is rotationally connected with a plurality of sloping plates (12) through torsion springs; each inclined plate (12) is fixedly connected with a plurality of slip rings (13), the transfusion hose (11) is in sliding connection with the slip rings (13), and the transfusion hose (11) is communicated with the nutrient solution conveying mechanism; the box body (1) is connected with a constant temperature component for controlling temperature;
the sloping plate (12) is provided with an arc part (12 a);
The device also comprises a wafer (121) and an ultraviolet lamp (14); two circular discs (121) are fixedly connected at the pipe orifice of each transfusion hose (11); the inner wall of the cylinder II (8) is respectively provided with a plurality of ultraviolet lamps (14) for sterilization and disinfection;
The device also comprises a connecting rod (131), a fixed ring (132) and a lantern ring (133); the limiting ring (10) is fixedly connected with a plurality of connecting rods (131); all the connecting rods (131) are fixedly connected with a fixed ring (132); the fixing ring (132) is fixedly connected with a collar (133), the fixing ring (132) is positioned at the outer side part of the collar (133), and the collar (133) is made of flexible materials; the bottom of the lantern ring (133) is provided with a groove (133 a);
The device also comprises an elastic telescopic rod (141), a trapezoid block (142), a film (143) and a clamping plate (144); a plurality of elastic telescopic rods (141) are fixedly connected at the placement holes of each placement plate (4); each elastic telescopic rod (141) is fixedly connected with a trapezoid block (142); each trapezoid block (142) is fixedly connected with a clamping plate (144); the placing holes of each placing plate (4) are fixedly connected with a plurality of films (143), the films (143) are fixedly connected with clamping plates (144), and the films (143) wrap the elastic telescopic rods (141) and the trapezoid blocks (142).
2. A conveniently stored incubator according to claim 1, characterized in that: the device also comprises a servo motor (111), a screw rod (112), a movable plate (113) and an elastic membrane (114); the box body (1) is fixedly connected with a servo motor (111); the box body (1) is rotationally connected with a screw rod (112), and the screw rod (112) is fixedly connected with an output shaft of the servo motor (111); the screw rod (112) is connected with a movable plate (113) in a sliding way; the movable plate (113) is fixedly connected with an elastic membrane (114), and the elastic membrane (114) is fixedly connected with the inner wall of the box body (1).
3. A conveniently stored incubator according to claim 1, characterized in that: the device also comprises a placing platform (201), a cleaning box (202), a sterilizing box (204) and a sponge (203); the box body (1) is fixedly connected with a placement platform (201); the placing platform (201) is provided with a cleaning box (202); a sponge (203) is arranged on the cleaning box (202); the disinfection box (204) is arranged on the placement platform (201), disinfection liquid is added into the disinfection box (204), and an ultrasonic cleaning mechanism is arranged inside the disinfection box.
4. A conveniently stored incubator according to claim 3, characterized in that: the sponge (203) is provided with protrusions (203 a).
5. A conveniently stored incubator according to claim 4, wherein: the constant temperature component comprises a temperature regulating box (301), an air inlet pipe (302), an air outlet pipe (303) and a temperature detector (304); the box body (1) is fixedly connected with a temperature regulating box (301), and a hot air mechanism and a cold air mechanism for controlling the temperature are arranged in the temperature regulating box (301); the temperature regulating box (301) is communicated with an air inlet pipe (302), and the air inlet pipe (302) is communicated with the box body (1); the temperature adjusting box (301) is communicated with an air outlet pipe (303), and the air outlet pipe (303) is communicated with the box body (1); a temperature detector (304) is arranged inside the box body (1).
6. A conveniently stored incubator according to claim 5, wherein: the device also comprises a first connecting pipe (311), a diaphragm (312) and a second connecting pipe (314); the box body (1) is fixedly connected with a first connecting pipe (311), the first connecting pipe (311) is communicated with the air inlet pipe (302), and the first connecting pipe (311) is communicated with the inside of the bearing plate (5); the box body (1) is fixedly connected with a second connecting pipe (314), the second connecting pipe (314) is communicated with the air outlet pipe (303), and the second connecting pipe (314) is communicated with the inside of the bearing plate (5); a diaphragm (312) is arranged at each placing groove (5 a), and the diaphragm (312) is positioned above the pressure sensor (313).
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| CN202410210730.8A CN117801928B (en) | 2024-02-27 | 2024-02-27 | Constant temperature incubator convenient to store |
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