CN117760790A - Automatic sucking device for egg white samples - Google Patents

Abstract

The invention relates to the field of machine manufacturing, in particular to an automatic sucking device for an egg white sample. Comprising the following steps: the machine body, but the operation storehouse of open and close is installed to the organism top, the operation storehouse bottom is fixed with the mounting surface, still includes first track, first track is fixed on the mounting surface, sliding connection has first sliding block on the first track, first sliding block top fixedly connected with second track, first sliding block is used for driving the second track and slides along first track, sliding connection has the second sliding block on the second track, second sliding block fixedly connected with third track, the second sliding block is used for driving third track and slides along the second track, sliding connection has the third sliding block on the third track, install the pipettor that is used for absorbing egg white on the third sliding block, first track sets up along the fore-and-aft direction, has solved the manual work and has been low to egg white pipetting efficiency, and sample interval time is long, leads to the problem that detection error is big.

Description

Automatic sucking device for egg white samples

Technical Field

The invention relates to the field of machine manufacturing, in particular to an automatic sucking device for an egg white sample.

Background

The fowl leukemia is a common name of various chicken tumor diseases caused by fowl leukemia virus, and the main prevention and control means is to prevent the transmission of fowl leukemia virus by means of seed source purification because the diseases have no vaccine and special medicine. In China, white feather chickens and imported laying hens basically realize the control of avian leukemia, part of the original egg-laying chickens bred by self are purified, but local varieties such as yellow feather chickens still need to be comprehensively monitored and purified. For the purification process of the avian leukosis, the strictest purification process can be adopted, for example, p27 antigen ELISA kit is adopted for detecting the embryo manure of 1 day old chicks, 2 hen eggs which are collected at the beginning of 23-25 weeks old are subjected to egg white p27 antigen detection, and the semen is collected by a reserved cock for detection, etc. Wherein the egg white is derived from eggs laid by the breeding hens, and the egg white can judge whether the breeding hens are infected with the avian leukosis virus or not by detecting the specific antigen in the egg white, and the infected breeding hens are eliminated, so that the aim of purifying is fulfilled.

Currently, avian leukemia virus antigens are detected by ELISA methods, with egg white samples being one of the important subjects to be detected. At present, egg white samples are sucked and added to an ELISA reaction plate in a manual mode, the working efficiency of the mode is low (more than 10 minutes/plate), the sample is easy to be misplaced, and the detection error is large due to long ELISA reaction time intervals of the front and rear samples.

The invention patent with the application number of 201610628011.3 provides an automatic egg yolk-egg white separation system, which belongs to the field of food processing equipment, and comprises an egg conveying device for conveying eggs, an egg breaking device for separating egg liquid from egg shells and an egg yolk-egg white separation device for separating egg yolk from egg white, wherein the egg breaking device comprises a rotatable turntable, an egg breaking assembly and a positioning assembly, the positioning assembly comprises a positioning shaft and egg supporting brackets positioned at two sides of the positioning shaft, the egg supporting brackets can rotate around the positioning shaft, one end of each egg supporting bracket extends out of a concave part outside the turntable, and a gap is reserved between the two egg supporting brackets; the egg breaking assembly comprises a lifting workbench, two egg breaking knives capable of moving in parallel are arranged on the workbench, and when the egg breaking knives are positioned below the egg supporting part, the workbench is lifted to drive the two egg breaking knives to pass through the gap. The egg yolk-egg white automatic separation system provided by the invention can efficiently perform egg yolk-egg white separation operation, and is simple in structure and convenient to use.

The egg white is separated from egg white mechanically by the technology, the egg white is separated from the egg yolk in a seepage way, some egg yolk possibly is doped in the unavoidable egg white, so that the egg white is impure, the egg white used for detecting the avian leukosis virus antigen by an ELISA method is clean and cannot be mixed with the egg yolk, otherwise, the sample purity is insufficient, the detection result is inaccurate, the egg white is sucked and transferred manually, the working efficiency is low, the sampling time interval of the front sample and the rear sample is large, the detection result is inaccurate, and a technology is needed to quickly transfer the egg white.

Disclosure of Invention

The invention provides an automatic egg white sample sucking device, which can solve the problems of low working efficiency of manually pipetting egg white, long sample interval time and large detection error.

In order to solve the technical problems, the application provides the following technical scheme:

an automated collection device for a protein sample, comprising: the machine body, but the operation storehouse of open and close is installed to the organism top, the operation storehouse bottom is fixed with the mounting surface, still includes first track, first track is fixed on the mounting surface, sliding connection has first sliding block on the first track, first sliding block top fixedly connected with second track, first sliding block is used for driving the second track and slides along first track, sliding connection has the second sliding block on the second track, second sliding block fixedly connected with third track, the second sliding block is used for driving the third track and slides along the second track, sliding connection has the third sliding block on the third track, install the pipettor that is used for absorbing the egg white on the third sliding block, first track sets up along the fore-and-aft direction, and the second track sets up along the left-right direction, and the third track sets up along upper and lower direction, and first track, second track, third track are two liang mutually perpendicular.

The basic principle and the beneficial effects of the scheme are that: adopt mechanized transportation mode, first track, second track, the mutual cooperation between the third track can be to the egg white that needs to shift from the optional position in the operation storehouse space scope to other appointed positions, and this scheme does not have artificial participation, has reduced operating error, has practiced thrift the ageing, has reduced the sampling time error of sample, has guaranteed the accuracy of testing result. Can improve sample suction efficiency, avoid the application of error appearance, guarantee reaction time unanimous to can leave a kind review etc. makes the operation flow unified standard, improves egg white and detects the precision, reinforcing purifying effect. The problem of the manual work is low to egg white pipetting efficiency, and sample interval time is long, leads to detecting error big is solved.

Further, the travel of the third track is 150-220mm, and the travel of the second track is 200-700mm.

Further, the first sliding block, the second sliding block and the third sliding block are all driven by an air cylinder or a sliding screw structure.

Further, still be provided with the platform of placing the egg in the operation storehouse, place the below of platform setting in the initial position of third sliding block, still include the reaction plate, the reaction plate sets up the below at the termination point of third sliding block, the third sliding block drives the pipettor and absorbs the egg white from the egg department of placing the platform and transport to the reaction plate.

Further, the placing table is provided with 6-10 egg placing grooves with uniform intervals, and the number of the reaction plates and the number of the pipettors are consistent with the number of the egg placing grooves.

The beneficial effects are that: the plurality of pipettors can be operated synchronously, so that the operation can be performed rapidly and in batches, and the time is saved.

Further, the pipettor comprises a pipetting needle and a pipette, wherein the pipetting needle is used for sucking egg white through an eggshell of an egg placed on a table, temporarily storing the egg white in the pipette and transferring the egg white on a reaction plate.

Further, the pipetting needle head comprises a puncture head, a pipetting needle head and a pipetting block, wherein the pipetting block is in an hourglass shape, the puncture head is used for puncturing an eggshell, the puncture head is fixed at the bottom of the pipetting block, a pipetting channel is arranged in the pipetting block and communicated with a pipette, an inlet of the pipetting channel is arranged in a concave position of the pipetting block, and the pipetting needle head is arranged at an inlet of the pipetting channel and communicated with the pipetting channel.

The beneficial effects are that: for egg white sampling, a notch is formed on an egg shell by adopting an egg opener, and then the egg white is sampled by adopting a liquid transfer device, but the process needs a long time for egg shell opening, particularly when in batch operation, the process of egg shell opening needs to waste a long time, and the egg shells are opened one by one, the time interval between the first opened egg and the last opened egg is too long, and the egg white of the first opened egg is possibly deteriorated due to sampling. The structure of change pipetting needle is selected in this scheme, sets up a puncture head in pipetting needle's bottom, is used for breaking down the eggshell specially, then the pipetting needle is inside entering the egg along with the puncture head through the hole that the puncture head opened on the eggshell, and the pipetting needle absorbs egg white to the pipette, and this process has prevented the eggshell from blocking the condition of syringe needle, if do not set up the pjncture needle, directly punctures with current syringe needle, probably the eggshell can block in the needle, influences the later stage imbibition, leads to the sample to can not accomplish. Or a part of the sample is blocked in the needle head, so that the sampling amount does not reach the standard in the same time, and the sampling fails. The above problems can be avoided through the arrangement of the scheme.

Further, the puncture head is of a solid structure, and the bottom is sharp.

Further, the bottom of standing groove is provided with the light lamp, still be provided with the camera in the operation storehouse, open the light lamp before the pjncture needle gets into the egg, the camera catches the picture of egg after shining by the light lamp to transmit to data processing module analysis, data processing module judges yolk position according to the shadow of picture, then calculates angle and the degree of depth that the liquid-transfering needle got into the egg, and the first sliding block, second sliding block, the third sliding block of control remove to appointed position and accomplish the egg white sample.

The beneficial effects are that: preventing the insertion of the pipetting needle into the yolk, resulting in contamination of the sampled specimen.

Further, the first track and the first sliding blocks are two, the two first tracks and the two first sliding blocks are oppositely arranged along the two ends of the second track, and the height of the first sliding blocks is adjustable.

Drawings

FIG. 1 is a perspective view of an embodiment of an automated egg white sample aspiration device;

FIG. 2 is a front view of an automated egg white sample extraction device;

FIG. 3 is a schematic view of a pipette;

fig. 4 is an enlarged schematic view at a in fig. 3.

Detailed Description

The following is a further detailed description of the embodiments:

the labels in the drawings of this specification include: 1. a body; 21. a first track; 22. a second track; 23. a third rail 23; 24. a first slider; 25. a second slider; 26. a third slider; 3. a pipette; 31. a pipetting needle; 311. a puncture head; 312. a liquid sucking needle; 313. a liquid suction block 313; 32. a pipette; 33. a pipetting block; 4. a reaction plate; 5. placing a table.

An embodiment as shown in figure 1 of the drawings,

an automated collection device for a protein sample, comprising: the machine body 1, but the operation storehouse of open-close is installed to organism 1 top, and the operation storehouse is the box, and the four sides all is provided with the baffle, and wherein the baffle can be opened around, is the appearance after opening in fig. 1, and the operation is sealed at the during operation in the operation storehouse, and does aseptic processing, the operation storehouse bottom is fixed with the mounting plane, and the mounting plane is the upper plane of organism 1.

The egg white sucking device is characterized by further comprising a first rail 21, wherein the first rail 21 is fixed on an installation plane, a first sliding block 24 is connected onto the first rail 21 in a sliding manner, a second rail 22 is fixedly connected onto the first rail 24, the first sliding block 24 is used for driving the second rail 22 to slide along the first rail 21, a second sliding block 25 is connected onto the second rail 22 in a sliding manner, a third rail 23 is fixedly connected onto the second sliding block 25, the second sliding block 25 is used for driving the third rail 23 to slide along the second rail 22, a third sliding block 26 is connected onto the third rail 23 in a sliding manner, a liquid shifter 3 for sucking is arranged on the third sliding block 26, the first rail 21 is arranged along the front-back direction, the second rail 22 is arranged along the left-right direction, the third rail 23 is arranged along the upper-down direction, the first rail 21, the second rail 22 and the third rail 23 are perpendicular to each other, the first rail 21 and the first sliding block 24 are provided with two rails 21 and two opposite to each other, and the two first sliding blocks 24 are arranged at two ends of the two opposite first rails 24 and two opposite to each other, and the two first sliding blocks 24 are arranged at two ends of the first sliding blocks 24 are height-adjustable. The first rails 21 are disposed side-by-side to provide stable support for the second rails 22.

The travel of the third rail 23 is 150-220mm, and the travel of the second rail 22 is 200-700mm. The third rail 23 corresponds to the z-axis in three-dimensional coordinates, the second rail 22 corresponds to the y-axis, and extends in a spatially oriented manner, and the corresponding third slide block 26 above it slides in an oriented manner with the second slide block 25. Each pipette 3 satisfies the following settings, with a pipetting range of 20-1000ul, at least guaranteeing pipetting of 1000ul. Sample addition accuracy is 100ul: cv is less than or equal to 1 percent, and accuracy is +/-2 percent; sample adding precision is 1000ul, cv is less than or equal to 0.5%, accuracy is +/-1%, and pipetting channel consistency is achieved: not more than 5% of the set transfer amount.

The first sliding block 24, the second sliding block 25 and the third sliding block 26 are all driven by an air cylinder or a sliding screw structure. In this scheme, the ball screw structure is adopted to drive, namely first sliding block 24, second sliding block 25, third sliding block 26 are all fixed with a ball seat, all install the lead screw on first track 21, second track 22, the third track 23, ball seat and lead screw correspond the installation, and the lead screw is driven by servo motor.

As shown in fig. 2, the operation bin is further provided with a placement table 5 for placing eggs, the placement table 5 is arranged below the initial position of the third sliding block 26, the operation bin further comprises a reaction plate 4, the reaction plate 4 is arranged below the end position of the third sliding block 26, and the third sliding block 26 drives the pipettor 3 to suck egg white from the eggs placed on the placement table 5 and then transfer the egg white to the reaction plate 4.

The egg placing table 5 is provided with 6-10 egg placing grooves with uniform intervals, and the number of the reaction plates 4 and the number of the pipettors 3 are consistent with the number of the egg placing grooves. As shown in fig. 2-3, 8 egg placing grooves are selected in the scheme, namely, the pipetting operation of 8 parts of egg white can be completed at one time. A flexible variable-pitch channel is arranged between two adjacent pipettors 3, namely, the distance between the two adjacent pipettors 3 is adjustable, the minimum distance of each flexible variable-pitch channel is 9mm, and the maximum distance of 40mm can be realized through equidistant conversion.

As shown in fig. 3 to 4, the pipette 3 comprises a pipette needle 31 and a pipette 32, wherein the pipette needle 31 is used for sucking egg white through an eggshell of an egg on the placement table 5, temporarily storing the egg white in the pipette 32 and transferring the egg white on the reaction plate 4. The pipetting needle head 31 comprises a puncture head 311, a pipetting needle head 312 and a pipetting block 313, the pipetting block 313 is in an hourglass shape, the puncture head 311 is used for puncturing eggshells, the puncture head 311 is fixed at the bottom of the pipetting block 313, a pipetting channel is arranged in the pipetting block 313 and is communicated with the pipette 32, an inlet of the pipetting channel is arranged in a concave part of the pipetting block 313, and the pipetting needle head 312 is arranged at an inlet of the pipetting channel and is communicated with the pipetting channel. The puncture head 311 is of a solid structure, the bottom is sharp, the puncture head 311 is of a solid steel structure, the hardness is high, and the puncture head can penetrate through eggshells. A thumb motor is arranged between the pipetting block 33 and the third sliding block 26 on each pipetter 3, and the pipetting needle 31 and the pipette 32 are fixed on the pipetting block 33, namely, the thumb motor can operate the pipetting block 33 to change the angle, so that sampling is facilitated.

The bottom of standing groove is provided with the light, still be provided with the camera in the operation storehouse, open the light before the pjncture needle gets into the egg, the camera catches the picture of egg after being shone by the light to data processing module analysis is transmitted, and data processing module judges yolk place position according to the shadow of picture, then calculates angle and the degree of depth that pipetting needle 31 got into the egg, and the egg white sample is accomplished to the first sliding block 24 of control, second sliding block 25, third sliding block 26 removal to appointed position. For egg white sampling, a notch is formed on an egg shell by adopting an egg opener, and then the egg white is sampled by using a liquid transfer device 3, but the process needs a long time for egg shell opening, particularly when in batch operation, the process of egg shell opening needs to waste a long time, and the time interval between the first opening and the last opening of the egg is too long, and the egg white of the first opening of the egg is possibly deteriorated due to sampling. The structure of the pipetting needle 31 is selectively changed in the scheme, the puncture head 311 is arranged at the bottom of the pipetting needle 31 and is specially used for puncturing eggshells, then the pipetting needle 312 enters the egg through a hole formed in the eggshells by the puncture head 311 along with the puncture head 311, the pipetting needle 312 sucks egg white into the pipette 32, the eggshells are prevented from being blocked by the pipetting needle, if the puncture needle is not arranged, the eggshells are directly punctured by the existing needle, the eggshells are likely to be blocked in the needle, the later pipetting is affected, and the sampling cannot be completed. Or a part of the sample is blocked in the needle head, so that the sampling amount does not reach the standard in the same time, and the sampling fails. The above problems can be avoided through the arrangement of the scheme.

The foregoing is merely an embodiment of the present invention, the present invention is not limited to the field of this embodiment, and the specific structures and features well known in the schemes are not described in any way herein, so that those skilled in the art will know all the prior art in the field before the application date or priority date, and will have the capability of applying the conventional experimental means before the date, and those skilled in the art may, in light of the teaching of this application, complete and implement this scheme in combination with their own capabilities, and some typical known structures or known methods should not be an obstacle for those skilled in the art to practice this application. It should be noted that modifications and improvements can be made by those skilled in the art without departing from the structure of the present invention, and these should also be considered as the scope of the present invention, which does not affect the effect of the implementation of the present invention and the utility of the patent. The protection scope of the present application shall be subject to the content of the claims, and the description of the specific embodiments and the like in the specification can be used for explaining the content of the claims.

Claims (10)

1. Automatic equipment of absorbing of egg white sample, its characterized in that includes: the machine body, but the operation storehouse of open and close is installed to the organism top, the operation storehouse bottom is fixed with the mounting surface, still includes first track, first track is fixed on the mounting surface, sliding connection has first sliding block on the first track, first sliding block top fixedly connected with second track, first sliding block is used for driving the second track and slides along first track, sliding connection has the second sliding block on the second track, second sliding block fixedly connected with third track, the second sliding block is used for driving the third track and slides along the second track, sliding connection has the third sliding block on the third track, install the pipettor that is used for absorbing the egg white on the third sliding block, first track sets up along the fore-and-aft direction, and the second track sets up along the left-right direction, and the third track sets up along upper and lower direction, and first track, second track, third track are two liang mutually perpendicular.

2. An automated egg white sample extraction apparatus as defined in claim 1, wherein: the travel of the third track is 150-220mm, and the travel of the second track is 200-700mm.

3. An automated egg white sample extraction apparatus as defined in claim 1, wherein: the first sliding block, the second sliding block and the third sliding block are all driven by an air cylinder or a sliding screw structure.

4. An automated egg white sample extraction apparatus as defined in claim 1, wherein: still be provided with the platform of placing the egg in the operation storehouse, place the platform setting in the initial position's of third sliding block below, still include the reaction plate, the reaction plate setting is in the below of the termination point of third sliding block, the third sliding block drives the pipettor and absorbs the egg white from the egg department of placing the platform and transport to the reaction plate.

5. An automated egg white sample extraction apparatus as defined in claim 4, wherein: the egg placing table is provided with 6-10 egg placing grooves with uniform intervals, and the number of the reaction plates and the number of the pipettors are consistent with the number of the egg placing grooves.

6. An automated egg white sample extraction apparatus as defined in claim 5, wherein: the pipettor comprises a pipetting needle head and a pipette, wherein the pipetting needle head is used for sucking egg white through an eggshell of an egg placed on a table, temporarily storing the egg white in the pipette and transferring the egg white on a reaction plate.

7. The automated protein sample extraction apparatus of claim 6, wherein: the pipetting needle head comprises a puncture head, a pipetting needle head and a pipetting block, wherein the pipetting block is in an hourglass shape, the puncture head is used for puncturing an eggshell, the puncture head is fixed at the bottom of the pipetting block, a pipetting channel is arranged in the pipetting block and communicated with a pipette, an inlet of the pipetting channel is arranged in a concave position of the pipetting block, and the pipetting needle head is arranged at the inlet of the pipetting channel and communicated with the pipetting channel.

8. An automated egg white sample extraction apparatus as defined in claim 7, wherein: the puncture head is of a solid structure, and the bottom is sharp.

9. The automated protein sample extraction apparatus of claim 8, wherein: the bottom of standing groove is provided with the light lamp, still be provided with the camera in the operation storehouse, open the light lamp before the pjncture needle gets into the egg, the camera catches the picture of egg after shining by the light lamp to transmit to data processing module analysis, data processing module judges yolk place position according to the shadow of picture, then calculates angle and the degree of depth that the liquid-transfering needle head got into the egg, and the first sliding block of control, second sliding block, third sliding block remove to appointed position and accomplish the egg white sample.

10. An automated egg white sample extraction apparatus as defined in claim 1, wherein: the first track and the first sliding blocks are two, the two first tracks and the two first sliding blocks are oppositely arranged along the two ends of the second track, and the height of the first sliding blocks is adjustable.