CN218995381U

CN218995381U - Multi-sample detection glass plate and detection device for glass plate agglutination test

Info

Publication number: CN218995381U
Application number: CN202222329425.XU
Authority: CN
Inventors: 范建华; 刘梅; 张莹; 俞燕; 贾雪波; 戴亚斌
Original assignee: Jiangsu Institute Poultry Sciences
Current assignee: Jiangsu Institute Poultry Sciences
Priority date: 2022-09-02
Filing date: 2022-09-02
Publication date: 2023-05-09
Anticipated expiration: 2032-09-02

Abstract

The utility model relates to the technical field of biological detection, and discloses a glass plate for multi-sample detection and a detection device based on a glass plate agglutination test. The glass plate is provided with a plurality of sample adding areas, and the intervals of the centers of the sample adding areas are matched with the intervals of one or more gun heads of the multi-channel pipettor. The same multi-channel pipettor is adopted to simultaneously drip the detection antigens into the corresponding sample adding areas, so that the detection efficiency is improved, the detection time is saved, and the method is particularly suitable for detecting and diagnosing diseases such as pullorum disease and the like in general chicken farms and laboratories under experimental conditions.

Description

Multi-sample detection glass plate and detection device for glass plate agglutination test

Technical Field

The utility model relates to the technical field of biological detection, in particular to a multi-sample detection glass plate and a detection device based on a glass plate agglutination test.

Background

Pullorum is an infectious disease caused by salmonella pullorum, is one of the most serious diseases endangering the chicken industry, and particularly for breeder chickens, higher morbidity and mortality occur in chicken farms in recent years through egg-borne vertical transmission and amplification effects. Because specific vaccines are not available and drug resistance is easy to generate, the most effective prevention and control means for pullorum disease is to detect and eliminate positive chicken flocks.

The method for detecting pullorum disease in laboratory is a detection method which is most popular to be applied in chicken farms in the present stage because of the advantages of simple operation, time saving, low detection cost and the like of the whole blood glass plate agglutination reaction test, and related standards are formulated in China and industry. The glass plate structure used for the agglutination reaction in the prior art is more complex, and although a plurality of sample adding areas are arranged, the positions of the sample adding areas are arranged randomly, and the conventional detection can only add a single sample into a certain sample adding area, so that only one sample can be detected at a time. The agglutination detection plate disclosed in patent CN203595718U is provided with more than one reaction tank, the reaction tank is a cylindrical groove, wherein only the diameter of the reaction tank is 6-16mm, the depth is 3-8mm, the inner surface of the reaction tank is subjected to mirror polishing treatment, how the reaction tanks are arranged between adjacent reaction tanks is not involved, and the reaction tank can only be added one by one for detection during sample addition. When the detection result is not easy to read negative or positive reaction, the detection is repeated again. When a large number of detection samples are encountered, single sample detection obviously requires a large amount of detection time, and accuracy cannot be guaranteed.

Disclosure of Invention

The utility model aims to provide a glass plate for detecting multiple samples and a glass plate agglutination detection device, which can simultaneously carry out sample adding detection on multiple serum samples, shorten detection time and improve detection efficiency.

In order to achieve the above purpose, the present utility model adopts the following technical scheme:

the utility model provides a multi-sample detection glass plate, which is provided with a plurality of sample adding areas, wherein the intervals of the aperture centers of the sample adding areas are matched with the intervals of 4 gun heads of a multi-channel pipette.

Preferably, the sample adding areas are arranged horizontally and vertically, and 4 sample adding areas are arranged vertically.

Preferably, the columns of the sample application areas are arranged at equal intervals, and the columns of the sample application areas are arranged at equal intervals or at unequal intervals.

Preferably, the sample adding centers of each row of the sample adding region are respectively spaced by 3cm, and the sample adding centers of each column are respectively spaced by 2.8cm, 3.6cm and 3.6cm.

Preferably, the sample adding area is circular and has a diameter of 1.5-2cm.

Preferably, the glass plate has a size of 15cm by 20cm.

Preferably, a substrate is arranged under the glass plate, and marks are arranged on the substrate to distinguish each sample adding area.

Preferably, the glass plate is provided with a negative blood serum sample adding area and a positive blood serum sample adding area.

The utility model also provides a detection device based on the glass plate agglutination test, which comprises the multi-sample detection glass plate and a plurality of pipettors.

Preferably, the multi-channel pipette is provided with 4 gun heads.

The glass plate can be repeatedly used for detecting the glass plate agglutination test after wiping and sterilizing. As one embodiment, the glass plate is cleaned by wiping with a 75% alcohol cotton ball and then air dried.

Compared with the prior art, the utility model has the following advantages and effects:

the multi-sample detection glass plate and the glass plate agglutination detection device can detect 4 serum samples at one time, the actual operability of detection is stronger, the detection time is shortened, the detection efficiency and the intuitiveness are improved, and the multi-sample detection glass plate and the glass plate agglutination detection device are particularly suitable for detecting and diagnosing diseases such as pullorum disease and the like in general chicken farms and laboratories under experimental conditions.

Drawings

FIG. 1 is a schematic view of a glass panel;

FIG. 2 is a schematic view of the distance between the sample loading areas of the glass plate;

FIG. 3 is a detection device based on a glass plate agglutination test;

wherein 1 is a glass plate; 2 is a sample adding area; 21 is a negative serum sample addition zone; 22 is positive serum loading zone; 3 is a bottom lining; 4 is a multi-channel pipette.

Detailed Description

The utility model provides a multi-sample detection glass plate, wherein a plurality of sample adding areas 2 are arranged on a glass plate 1, and the intervals at the centers of the sample adding areas 2 are matched with 4 intervals of a plurality of pipettes 4. Therefore, the same multi-channel liquid dispenser is adopted to simultaneously drop the detection antigens into the corresponding sample adding areas, so that the detection efficiency is improved, and the detection time is saved. The utility model is not particularly limited to the specific type and model of the multichannel pipettor 4, and specifically relates to 4, 8 and 12 channel pipettors, wherein the multichannel pipettors can be manual or electric, and the spacing of the multichannel pipettors can be adjustable or not.

As an embodiment, the sample application area 2 is arranged horizontally and vertically. As an embodiment, the sample application areas 2 are arranged at equal intervals, such as 3cm, for each row. As one implementation mode, the intervals of the columns of the sample application areas 2 are equidistant or non-equidistant so as to match the intervals of the gun heads of the corresponding multi-channel pipettes 4, such as the intervals of 2.8cm, 3.6cm and 3.6cm of the sample application centers of the columns of the sample application areas 2 respectively.

The sample adding area 2 of the glass plate 1 is circular, the diameter size accords with the coating size of antigen and serum, and the diameter of the optional sample adding area 2 is 1.5-2cm, preferably 1.8cm.

The source and the manufacturing mode of the glass plate 1 are not particularly limited, and the glass plate 1 can be manufactured by cutting a common glass plate, wherein the size of the glass plate 1 can be determined according to the number of samples, and the optional size is 15cm multiplied by 20cm.

As an embodiment, the bottom surface of the glass plate 1 of the present utility model is further provided with a bottom liner 3, and the bottom liner 3 is provided with marks to distinguish each sample application area 2. The backing 3 is optionally paper, board, lining cloth or the like, preferably paper with a certain hardness, such as A4 paper.

As one embodiment, the same glass plate 1 is provided with a negative serum sample application area 21 and a positive serum sample application area 22. The specific positions of the negative and positive serum sample-applying

regions

21 and 22 are not particularly limited, and any position of the sample-applying region 2 on the glass plate 1 may be selected.

The utility model also provides a detection device based on the glass plate agglutination test, which comprises the multi-sample detection glass plate 1 and a plurality of pipettes 4. Preferably, the multi-channel pipette 4 is provided with 4 gun heads.

The utility model also provides a detection method based on the glass plate agglutination test, which comprises the following steps: collecting venous blood aseptically, and separating out serum; and (3) sucking serum by using a liquid-transfering device 4, dripping the serum into the sample-adding areas 2 of the glass plate 1, uniformly mixing the standard detection antigens and the serum by using a plurality of liquid-transfering devices 4, and simultaneously judging the detection results of the serum in each sample-adding area 2 within 2 minutes. Preferably, the antigen and serum are coated to a diameter not exceeding the diameter of the loading zone 2. Other detection modes refer to the existing national standard or industry standard, such as the pullorum disease antibody detection method and the SN/T1222-2003 standard of the whole blood plate agglutination test.

The multi-sample detection glass plate and the glass plate agglutination test detection device are suitable for the agglutination reaction detection of any antigen and any antibody, can detect a plurality of samples at one time, shorten the detection time, improve the detection efficiency and the intuitiveness, and are particularly suitable for detecting and diagnosing diseases such as pullorum disease in general chicken farms and laboratories under experimental conditions.

The present utility model will be further illustrated with reference to the following specific examples, but the present utility model is not limited to the following examples. The experimental methods used in the examples are conventional methods unless otherwise specified, and materials, reagents, etc. used, unless otherwise specified, are commercially available.

Example 1

As shown in FIG. 1, a multi-sample detection glass plate 1 is cut from a common transparent glass plate to a size of 15cm by 20cm. A plurality of sample adding areas 2 are arranged on the glass plate 1, and the intervals of the centers of the sample adding areas 2 are matched with the intervals of 4 gun heads of the multi-channel pipettor 4. The sample application centers of each row of the sample application region 2 are respectively spaced by 3cm, and the sample application centers of each column are respectively spaced by 2.8cm, 3.6cm and 3.6cm. The sample adding area 2 is round and has a diameter of 1.5-2cm. Under the glass plate 1, A4 paper is used as a substrate 3, and marks are arranged on the substrate 3 to distinguish each sample adding area 2. A negative serum sample adding area 21 and a positive serum sample adding area 22 are also arranged on the same glass plate 1.

A detection device (fig. 3) based on a glass plate agglutination test, comprising the above-mentioned multi-sample detection glass plate 1, and 12 pipettes 4.

A pullorum disease detection improvement method based on a glass plate agglutination test comprises the following steps:

step one, taking 1mL of blood from the lower vein of chicken wings by using a sterile injector, filling a 1.5mLEP tube, marking a sample number, standing the sample in a refrigerator at 4 ℃ overnight, and separating out serum;

cutting a plurality of common transparent glass plates according to the same size of 15cm multiplied by 20cm, cleaning the glass plates before use, wiping the surfaces with 75% alcohol cotton balls, airing, and placing the glass plates on A4 paper marked with a sample adding position. Sample adding positions marked on the A4 paper are respectively 2.8cm, 3.6cm and 3.6cm apart from sample adding centers of each longitudinal row, 3cm apart from sample adding centers of each transverse row, and 4 gun heads of the pipettes are inserted into gun head boxes according to 4 and interval rows;

step three, sucking 50 mu L of serum by a single-channel liquid-transferer into the first row of 4 sample-adding areas of the glass plate, inserting 4 gun heads which are placed at intervals by using the 12-channel liquid-transferer 4, sucking detection antigens from a liquid-adding groove, blowing and sucking the detection antigens with 4 serum samples, uniformly mixing the detection antigens, and coating the detection antigens into round liquid drops with the diameter of about 1.8 cm; a negative blood serum sample adding area 21 and a positive blood serum sample adding area 22 are simultaneously arranged on the same glass plate 1 as a comparison, and the operation method is the same as that described above;

and fourthly, rotating the glass plate to ensure that the serum to be detected and the antigen are fully and uniformly mixed, and simultaneously judging 4 serum detection results within 2 minutes.

And fifthly, judging the detection result. If the serum sample to be detected and the standard antigen are subjected to granular agglutination, the sample is positive, otherwise, the sample is negative, the sample and the standard antigen are considered to be suspicious, and the specific interpretation method refers to the SN/T1222-2003 standard.

The embodiments described above are preferred embodiments of the present utility model, but the embodiments of the present utility model are not limited to the embodiments described above, and any other changes, modifications, substitutions, combinations, and simplifications that do not depart from the spirit and principle of the present utility model should be made in the equivalent manner, and the embodiments are included in the scope of the present utility model.

Claims

1. The utility model provides a glass board is detected to multiple sample, its characterized in that, the glass board is provided with a plurality of application of sample district, 4 rifle head intervals of multichannel pipettor are matchd to the interval at application of sample district center.

2. The multiple sample detection glass panel of claim 1, wherein the sample application areas are disposed laterally and longitudinally, and 4 sample application areas are disposed longitudinally.

3. The multi-sample detection glass plate of claim 1, wherein the loading zone rows are arranged equidistantly, and the loading zone columns are arranged equidistantly or non-equidistantly.

4. A multi-sample detection glass plate according to any one of claims 1 to 3, wherein the sample application centers of each row of the sample application region are spaced apart by 3cm, and the sample application centers of each column are spaced apart by 2.8cm, 3.6cm, respectively.

5. The multiple sample detection glass plate of claim 1, wherein the sample addition zone is circular and has a diameter of 1.5-2cm.

6. The multiple sample detection glass panel of claim 1, wherein the glass panel has a size of 15cm x 20cm.

7. The multi-sample detection glass plate of claim 1, wherein a substrate is disposed under the glass plate, and a mark is disposed on the substrate to distinguish between the sample application regions.

8. The multi-sample detection glass plate according to claim 1, wherein a negative serum loading area and a positive serum loading area are provided on the glass plate.

9. A detection device based on a glass plate agglutination test, characterized in that the device comprises the multi-sample detection glass plate according to any one of claims 1 to 8, and a multi-channel pipette.

10. The device of claim 9, wherein the multichannel pipettor is provided with 4 tips.