CN117731712B - Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons - Google Patents
Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons Download PDFInfo
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Abstract
The invention relates to the field of traditional Chinese medicine, and provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which comprises the steps of rolling Ligusticum wallichii, radix salviae miltiorrhizae, fructus cnidii, kadsura pepper stem, cinnamon, liquorice, schizonepeta, bupleurum and polygonum cuspidatum into coarse powder, mixing with distilled water to prepare suspension, carrying out nanocrystallization grinding to obtain emulsion of traditional Chinese medicine materials, mixing with ethanol solution, carrying out reflux extraction, filtering and recovering ethanol from the supernatant after extraction, and concentrating to obtain an extract containing active ingredients of each traditional Chinese medicine component; mixing deer horn gelatin powder with ethanol solution as a solvent base, adding the traditional Chinese medicine extract prepared in the previous step into the solvent, adding the gentiana macrophylla extract, the stiff silkworm extract, the mistletoe extract and the basil leaf extract which are weighed according to mass percent into the mixture, stirring and concentrating until the alcohol content is reached, and finally preparing the traditional Chinese medicine tincture with the effects of activating blood and relaxing tendons, wherein the maximum extraction of active ingredients can be ensured, and the double effects of activating blood circulation and dissolving stasis and relaxing tendons and activating collaterals are considered.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines, in particular to a method for producing a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons.
Background
In the theory of traditional Chinese medicine, many diseases are related to unsmooth blood circulation and blocked tendons and veins. The Chinese medicine has the functions of promoting blood circulation to disperse blood clots, relaxing tendons and activating collaterals, and is one important Chinese medicine method for treating muscle pain, bone injury, rheumatic arthralgia and other symptoms. The traditional Chinese medicine has the advantages of utilizing the special efficacy of the natural medicinal materials, achieving the purposes of activating blood circulation to dissipate blood stasis and relaxing tendons and activating collaterals through various complex pharmacological actions, and treating various diseases caused by unsmooth qi and blood circulation.
The traditional Chinese medicine tincture mainly takes the forms of pills, tablets, ointment, powder and the like, and the preparations have wide clinical application. However, these traditional Chinese medicinal tinctures generally have the problems of long acting time, inconvenient carrying, uneven drug effect exertion, insufficient penetrating power and the like. In modern society, people are more and more pursuing therapeutic effects, and meanwhile, convenient and quick administration is also expected.
Tincture is favored as a liquid formulation for its rapid absorption rate and good penetration. The tincture is prepared by soaking Chinese medicinal materials in ethanol or ethanol solution to dissolve the effective components in the Chinese medicinal materials in solvent, and is administered orally or topically. Because ethanol has good dissolving capacity and high absorption speed, the tincture can play a role rapidly and is convenient to store and carry.
Despite the advantages, the tincture has the following defects: the formula of part of the products is single, and the drug effect is not comprehensive enough; the production process is not scientific enough, so that the extraction of the effective components is incomplete, and the like. Therefore, the development of a novel blood-activating and tendon-relaxing traditional Chinese medicine tincture and a scientific and reasonable production method thereof have important significance for meeting the health requirements of modern people.
Disclosure of Invention
The invention aims to provide a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by adopting a plurality of medicinal material compounds, has the double effects of promoting blood circulation and removing blood stasis and relaxing tendons and activating collaterals, optimizes the production process, ensures the maximum extraction of active ingredients, achieves the aim of quickly and effectively treating muscle pain and bone injury, and is convenient to carry and use.
In order to achieve the above purpose, the present invention provides the following technical solutions:
A method for producing a traditional Chinese medicine tincture for activating blood and relaxing tendons comprises the following steps:
Step one, preparing a traditional Chinese medicine extract;
and i, weighing the traditional Chinese medicine components according to the proportion: ligusticum wallichii, root of red rooted saliva, cnidium fruit, kadsura pepper stem, cinnamon, licorice root, schizonepeta, bupleurum root and giant knotweed;
ii, rolling the traditional Chinese medicine components with good weight into coarse powder, and sieving with a 60-80 mesh sieve; adding appropriate amount of distilled water into the obtained Chinese medicinal powder to obtain suspension, and uniformly performing nanocrystallization grinding treatment in a liquid grinder to obtain Chinese medicinal emulsion;
Iii, pouring each Chinese medicinal material emulsion into a container containing ethanol solution for reflux extraction for 2-3 times, filtering out supernatant fluid each time, recovering ethanol, and concentrating filtrate at 75 ℃ and under the condition of minus 0.8 to minus 0.6MPa to obtain a Chinese medicinal extract containing active ingredients of each Chinese medicinal component for later use;
Step two, preparing tincture;
The preparation method comprises the steps of weighing deer horn gelatin powder according to mass percentage, mixing the deer horn gelatin powder with ethanol solution to be used as a solvent, adding the prepared traditional Chinese medicine extract, and the gentiana macrophylla extract, the stiff silkworm extract, the mistletoe extract and the basil leaf extract which are weighed according to mass percentage, uniformly mixing under the magnetic stirring effect of stirring speed of 300-700 r/min, concentrating at 75 ℃, and preparing the traditional Chinese medicine tincture with 30-40% of alcohol content for promoting blood circulation and relaxing tendons.
As a further scheme of the invention, when each traditional Chinese medicine powder obtained in the step one is prepared into suspension, distilled water which is 3-8 times of the mass of each traditional Chinese medicine powder is added, and nano grinding is uniformly carried out in a liquid grinder at the temperature of 25 ℃ until the particle size is 30-100nm.
As a further scheme of the invention, the traditional Chinese medicine extract prepared in the first step comprises a ligusticum wallichii extract, a radix salviae miltiorrhizae extract, a fructus cnidii extract, a kadsura pepper stem extract, a cinnamon extract, a liquorice extract, a schizonepeta extract, a radix bupleuri extract and a polygonum cuspidatum extract.
As a further scheme of the invention, the preparation method of the ligusticum wallichii extract comprises the following steps:
Weighing a proper amount of ligusticum wallichii, rolling the ligusticum wallichii into coarse powder, sieving the coarse powder with a 60-80 mesh sieve, and adding 3 times of distilled water into the ligusticum wallichii powder to prepare suspension;
Performing nanocrystallization grinding treatment in a liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-70 nm;
Pouring the ligusticum chuanxiong hort emulsion into a container containing 75% ethanol solution for reflux extraction for 2-3 times, wherein each time period is 8-10 hours; filtering out supernatant, recovering ethanol, concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain rhizoma Ligustici Chuanxiong extract containing rhizoma Ligustici Chuanxiong effective components.
As a further scheme of the invention, when the ligusticum chuanxiong hort extract is prepared, the volume of the filtrate is concentrated to 1/10-1/20, and the ligusticum chuanxiong hort extract containing the effective components of the ligusticum chuanxiong hort is obtained.
As a further scheme of the invention, the blood-activating and tendon-relaxing traditional Chinese medicine tincture prepared in the second step comprises the following components in percentage by mass:
5% -9% of Ligusticum chuanxiong extract
5% -9% Of red sage root extract
3 To 7 percent of gentiana macrophylla extract
3 To 7 percent of cnidium fruit extract
3 To 5 percent of stiff silkworm extract
4% -6% Of mistletoe extract
3 To 7 percent of kadsura pepper stem extract
1 To 3 percent of deer horn gelatin powder
2% -6% Of cinnamon extract
1% -3% Of licorice extract
3 To 7 percent of schizonepeta extract
3 To 7 percent of bupleurum extract
3% -7% Of polygonum cuspidatum extract
2% -6% Of basil leaf extract
30% -40% Of ethanol.
As a further scheme of the invention, the preparation method of the gentiana macrophylla extract comprises the following steps:
Grinding radix Gentianae Marcrophyllae into powder, filtering with 60-80 mesh sieve, adding distilled water 3 times of the volume to obtain radix Gentianae Marcrophyllae suspension, and grinding with liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-100 nm;
Ethanol is selected as an organic solvent, gentiana macrophylla powder and ethanol are mixed according to the mass to volume ratio of 1:5 to 1:10, leaching is carried out for 8 to 10 hours at the temperature of 60 to 80 ℃, and reflux extraction is carried out for 2 to 3 times; filtering the extractive solution to remove solid residue, recovering ethanol to obtain solution containing radix Gentianae Marcrophyllae component, and concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain radix Gentianae Marcrophyllae extract.
As a further scheme of the invention, when the gentiana macrophylla powder and the ethanol are mixed according to the mass-to-volume ratio of 1:5 to 1:10, the solvent volume ratio of the weight of the medicinal materials of the gentiana macrophylla powder to the ethanol is adopted.
As a further scheme of the invention, the preparation method of the stiff silkworm extract comprises the following steps: grinding dried stiff silkworm into powder, adding 8 times of ethanol with the volume concentration of 75%, soaking for 10-15 minutes, heating in a water bath at 55-65 ℃ for 10-14 hours, reflux extracting for 2-3 times, heating and evaporating to remove ethanol, stirring at 300-800rpm for 20-60 minutes under the water bath condition of 40-60 ℃ for 1/10-1/20 of the volume concentration, and sterilizing to obtain the stiff silkworm extract.
As a further scheme of the invention, the preparation method of the mistletoe extract comprises the following steps: pulverizing dried stem and leaf of herba Taxilli into coarse powder, placing into supercritical CO 2 extraction device, adding 5 times volume of ethanol as entrainer, extracting under 35-65MPa at 25-75deg.C to obtain herba Taxilli extract.
Compared with the prior art, the invention has the beneficial effects that:
According to the production method of the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is provided by the invention, extracts of a plurality of traditional Chinese medicinal materials such as ligusticum wallichii, radix salviae miltiorrhizae, radix gentianae macrophyllae, fructus cnidii, stiff silkworm, herba taxilli, caulis kadsurae, cinnamon, liquorice, schizonepeta, radix bupleuri, polygonum cuspidatum and the like are combined with the deer antler gelatin powder and the basil leaf extract through ethanol reflux extraction and concentration, so that the tincture with the effects of promoting blood circulation and removing blood stasis, relaxing tendons and activating collaterals and diminishing inflammation and relieving pain can be produced, the effective components can be effectively extracted from traditional Chinese medicine materials, meanwhile, the effective components in the extract are not destroyed through controlling the pressure and the temperature, the concentration of the active components in the extract can be ensured, the final tincture has strong efficacy, the alcohol content of the prepared tincture is 30% -40%, the stability of the effective components can be ensured, the pollution of external microorganisms can be reduced, and the effective period of the medicine can be prolonged.
Detailed Description
The technical scheme of the invention is further described in detail below with reference to the specific embodiments.
Aiming at the problems of single prescription, incomplete efficacy and incomplete extraction of active ingredients caused by insufficient scientific production process of the blood-activating and tendon-relaxing tincture products on the market, the invention aims to develop a novel production method of the blood-activating and tendon-relaxing traditional Chinese medicine tincture, the blood-activating and tendon-relaxing traditional Chinese medicine tincture prepared by the method is prepared by adopting a plurality of medicinal material compounds, has double effects of activating blood circulation to dissipate blood stasis and relaxing tendons and activating collaterals, optimizes the production process, ensures the maximum extraction of the active ingredients, and simultaneously achieves the aims of rapidly and effectively treating muscle pain and bone injury, and is convenient to carry and use.
The application provides a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which comprises the following components in percentage by mass: 5-9% of ligusticum wallichii extract, 5-9% of red sage root extract, 3-7% of gentiana macrophylla extract, 3-7% of fructus cnidii extract, 3-5% of stiff silkworm extract, 4-6% of Chinese taxillus twig extract, 3-7% of kadsura pepper stem extract, 1-3% of deer horn gelatin powder, 2-6% of cinnamon extract, 1-3% of liquorice extract, 3-7% of schizonepeta extract, 3-7% of radix bupleuri extract, 3-7% of polygonum cuspidatum extract, 2-6% of basil leaf extract and 30-40% of ethanol.
In the application, if not specified, the gentiana macrophylla extract is prepared by grinding the gentiana macrophylla into powder, filtering the powder through a 80-mesh sieve, adding distilled water with 3 times of volume to prepare a gentiana macrophylla suspension, and grinding the suspension through a liquid grinder to obtain ligusticum chuanxiong emulsion with the particle size of 30-100 nm; ethanol is selected as an organic solvent, gentiana macrophylla powder and ethanol are mixed according to the proportion of 1:5, leaching is carried out for 10 hours at 60 ℃, and reflux extraction is carried out for 3 times; filtering the extractive solution to remove solid residue, recovering ethanol to obtain solution containing radix Gentianae Marcrophyllae component, concentrating the filtrate at 75deg.C and-0.6 MPa to obtain radix Gentianae Marcrophyllae extract.
Wherein, when the gentiana macrophylla powder and the ethanol are mixed according to the proportion of 1:5, the solvent volume ratio of the weight of the medicinal material of the gentiana macrophylla powder to the ethanol is adopted.
According to the steps, the traditional Chinese medicine components of the red sage root, the cnidium fruit, the kadsura pepper stem, the cinnamon, the liquorice, the schizonepeta, the bupleurum and the giant knotweed are respectively extracted to obtain the traditional Chinese medicine extract containing the effective components of the traditional Chinese medicine components: ligusticum wallichii extract, red sage root extract, cnidium fruit extract, kadsura pepper stem extract, cinnamon extract, licorice extract, schizonepeta extract, bupleurum extract and polygonum cuspidatum extract.
In the application, the stiff silkworm extract is prepared by grinding the stiff silkworm obtained into powder, adding ethanol with the volume concentration of 75% in an amount which is 8 times that of the powder, soaking for 15 minutes, heating in a water bath at 55 ℃ for 14 hours, extracting under reflux for 3 times, heating and evaporating to remove the ethanol, stirring at the stirring speed of 350rpm in a water bath at 60 ℃ for 50 minutes, concentrating to 1/10 by volume, and sterilizing.
In the preparation method of the mistletoe extract, dry stems and leaves of mistletoe are taken as raw materials and crushed into coarse powder, the coarse powder is placed in a supercritical CO 2 extraction device, ethanol with the volume of 5 times is added as entrainer, the extraction pressure is 50MPa, and the temperature is 45 ℃ to obtain the mistletoe extract.
The following will explain in conjunction with specific examples 1 to 5.
Wherein, each component of the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons of the example 1 to the example 5 and the dosage of each component are shown in the table 1 below, wherein, each component dosage in each tincture is g.
Table 1 examples 1-5 Each component and the amount (g) of each component of the blood-activating and tendon-relaxing Chinese medicinal tincture
| Component amount (g) | Example 1 | Example 2 | Example 3 | Example 4 | Example 5 |
| Ligusticum wallichii extract | 7.0 | 9.0 | 5.0 | 8.0 | 9.0 |
| Extract of red sage root | 7.0 | 5.0 | 5.0 | 7.0 | 9.0 |
| Gentiana macrophylla extract | 5.0 | 7.0 | 5.0 | 7.0 | 3.0 |
| Fructus Cnidii extract | 5.0 | 4.0 | 5.0 | 7.0 | 3.0 |
| Bombyx Batryticatus extract | 4.0 | 5.0 | 3.0 | 5.0 | 4.0 |
| Loranthus mulberry mistletoe extract | 5.0 | 6.0 | 5.0 | 5.0 | 4.0 |
| Piper hancei extract | 5.0 | 7.0 | 5.0 | 5.0 | 3.0 |
| Deer horn gelatin powder | 2.0 | 3.0 | 2.0 | 1.0 | 1.0 |
| Cinnamon extract | 4.0 | 2.0 | 5.0 | 3.0 | 6.0 |
| Licorice root extract | 2.0 | 3.0 | 2.0 | 1.0 | 3.0 |
| Herba Schizonepetae extract | 5.0 | 3.0 | 3.0 | 5.0 | 7.0 |
| Radix bupleuri extract | 5.0 | 6.0 | 5.0 | 7.0 | 3.0 |
| Polygonum cuspidatum extract | 5.0 | 5.0 | 5.0 | 3.0 | 7.0 |
| Basil leaf extract | 4.0 | 5.0 | 5.0 | 6.0 | 2.0 |
| Ethanol | 40.0 | 35.0 | 45.0 | 35.0 | 41.0 |
Specific examples and preparation methods are as follows:
Example 1
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
Step 1), putting 2g of deer horn gelatin powder and 40 g of ethanol into a medicine tank, stirring uniformly, and adding 7g of prepared ligusticum wallichii extract, 7g of red sage root extract, 5g of gentiana macrophylla extract, 5g of cnidium fruit extract, 4g of stiff silkworm extract, 5g of Chinese taxillus herb extract, 5g of kadsura pepper stem extract, 4g of cinnamon extract, 2g of licorice extract, 5g of schizonepeta extract, 5g of bupleurum root extract, 5g of polygonum cuspidatum extract and 4g of basil leaf extract.
Step 2), uniformly stirring at a speed of 350r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 35%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Example 2
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
Step 1), putting 3g of deer horn gelatin powder and 35 g of ethanol into a medicine tank, stirring uniformly, and adding 9g of prepared ligusticum wallichii extract, 5g of red sage root extract, 7g of gentiana macrophylla extract, 4g of cnidium fruit extract, 5g of stiff silkworm extract, 6g of Chinese taxillus herb extract, 7g of kadsura pepper stem extract, 2g of cinnamon extract, 3g of licorice extract, 3g of schizonepeta extract, 6g of bupleurum root extract, 5g of polygonum cuspidatum extract and 5g of basil leaf extract.
Step 2), uniformly stirring at a speed of 300r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 30%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Example 3
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
Step 1), putting 2g of deer horn gelatin powder and 45 g of ethanol into a medicine tank, stirring uniformly, and adding 5g of prepared ligusticum wallichii extract, 5g of red sage root extract, 5g of gentiana macrophylla extract, 5g of cnidium fruit extract, 3g of stiff silkworm extract, 5g of Chinese taxillus herb extract, 5g of kadsura pepper stem extract, 5g of cinnamon extract, 2g of licorice extract, 3g of schizonepeta extract, 5g of bupleurum root extract, 5g of polygonum cuspidatum extract and 5g of basil leaf extract.
Step 2), uniformly stirring at the speed of 700r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 40%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Example 4
The difference from example 2 is that: 1g of deer horn gelatin powder, 8g of szechuan lovage rhizome extract, 7g of red sage root extract, 7g of common cnidium fruit extract, 5g of Chinese taxillus herb extract, 5g of kadsura pepper stem extract, 3g of cinnamon extract, 1g of liquoric root extract, 5g of schizonepeta extract, 7g of bupleurum root extract, 3g of giant knotweed rhizome extract and 6g of basil leaf extract.
Example 5
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
step 1), putting 1g of deer horn gelatin powder and 41 g of ethanol into a medicine tank, stirring uniformly, and adding 9g of prepared ligusticum wallichii extract, 9g of red sage root extract, 3g of gentiana macrophylla extract, 3g of fructus cnidii extract, 4g of stiff silkworm extract, 4g of Chinese taxillus twig extract, 3g of kadsura pepper stem extract, 6g of cinnamon extract, 3g of liquorice extract, 7g of schizonepeta extract, 3g of bupleurum root extract, 7g of polygonum cuspidatum extract and 2g of basil leaf extract.
Step 2), uniformly stirring at a speed of 650r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 36%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Based on the above examples 1 to 5, referring to the amounts of the components shown in table 1, the rhizoma Ligustici Chuanxiong extract, saviae Miltiorrhizae radix extract, radix Gentianae Marcrophyllae extract, fructus Cnidii extract, bombyx Batryticatus extract, herba Taxilli extract and caulis Piperis Futokadsurae extract etc. required for preparing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons are weighed, when preparing, the deer-horn gelatin powder is weighed according to the mass percentage, the deer-horn gelatin powder is mixed with ethanol solution as a solvent, and the prepared traditional Chinese medicine extract, the radix Gentianae Marcrophyllae extract, the Bombyx Batryticatus extract, the herba Taxilli extract and the herba Ocimi leaf extract are added, and concentrated after mixing uniformly, the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons is prepared.
Comparative example
According to the difference from example 1, the respective weights of the ligusticum wallichii extract, the radix salviae miltiorrhizae extract and the gentiana macrophylla extract are different, and a blood-activating and tendon-relaxing traditional Chinese medicine tincture of comparative examples 1 to 3 is prepared, wherein the respective raw materials and the respective raw material dosages of the blood-activating and tendon-relaxing traditional Chinese medicine tincture of comparative examples 1 to 3 are shown in the following table 2, and the respective raw material dosages are g.
Table 2 comparative examples 1-3 various raw materials and the dosage (g) of the Chinese medicinal tincture for promoting blood circulation and relaxing tendons
| Component name | Comparative example 1 | Comparative example 2 | Comparative example 3 |
| Ligusticum chuanxiong extract/g | 7.0 | 7.0 | 7.0 |
| Salvia Miltiorrhiza extract/g | 7.0 | 7.0 | 7.0 |
| Radix Gentianae Marcrophyllae extract/g | / | 5.0 | 5.0 |
| Fructus Cnidii extract/g | 5.0 | 5.0 | 5.0 |
| Bombyx Batryticatus extract/g | 4.0 | / | 4.0 |
| Loranthus mulberry mistletoe extract/g | 5.0 | 5.0 | 5.0 |
| Kadsura pepper stem extract/g | 5.0 | 5.0 | 5.0 |
| Deer antler powder/g | 2.0 | 2.0 | 2.0 |
| Cinnamon extract/g | 4.0 | 4.0 | 4.0 |
| Glycyrrhrizae radix extract/g | 2.0 | 2.0 | 2.0 |
| Herba Schizonepetae extract/g | 5.0 | 5.0 | 5.0 |
| Radix bupleuri extract/g | 5.0 | 5.0 | 5.0 |
| Polygonum cuspidatum extract/g | 5.0 | 5.0 | 5.0 |
| Ocimum basilicum leaf extract/g | 4.0 | 4.0 | / |
| Ethanol/g | 40.0 | 40.0 | 40.0 |
Specific examples and preparation methods are as follows:
Referring to the components shown in Table 2 and the amounts of the components, the components required for preparing the blood-activating and tendon-relaxing traditional Chinese medicine tincture are weighed, and the difference between comparative example 1 and example 1 is that: the prepared traditional Chinese medicine tincture does not contain gentiana macrophylla extract, and the comparison example 2 is different from the example 1 in that: the prepared Chinese medicinal tincture does not contain stiff silkworm extract, and comparative example 3is different from example 1 in that: the prepared traditional Chinese medicine tincture does not contain the basil leaf extract, and the preparation method is the same as that of the example 1, so as to produce the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons.
Toxicity experiment: the Chinese medicinal tinctures prepared in example 1 and comparative examples 1 to 3 of the present invention were continuously administered to mice for 12 weeks and stopped for 4 weeks, wherein SD rats were randomly divided into 7 groups of 10 animals each, and named: blank, comparative example 1, comparative example 2, comparative example 3, example 1, experimental, low, medium, high dose tincture. Specific:
blank control group: the same volume of physiological saline was administered by gavage.
Comparative example 1 control group: the same volume of the traditional Chinese medicine tincture of the comparative example 1 is administrated by stomach irrigation, and the traditional Chinese medicine tincture is calculated according to the crude drug amount of 1.0ml/100g of body weight for 2 times per day.
Comparative example 2 control group: the same volume of the traditional Chinese medicine tincture of the comparative example 2 is administrated by stomach irrigation, and the traditional Chinese medicine tincture is calculated according to the crude drug amount of 1.0ml/100g body weight for 2 times per day.
Comparative example 3 control group: the same volume of the traditional Chinese medicine tincture of the comparative example 3 is administrated by stomach irrigation, and the traditional Chinese medicine tincture is calculated according to the crude drug amount of 1.0ml/100g body weight for 2 times per day.
Example 1 experimental group: the same volume of the traditional Chinese medicine tincture of the example 1 is administrated by stomach irrigation, wherein the weight of the traditional Chinese medicine tincture is 1.0ml/100g, and the crude drug amount is 2 times per day.
Low dose tincture group: the traditional Chinese medicine composition of the example 1 is administrated by stomach irrigation at a concentration of 0.5ml/100g, and the traditional Chinese medicine composition is calculated according to the crude drug amount.
Group of medium dose tinctures: 1.2ml/100g of the traditional Chinese medicine composition of the example 1 is administrated by stomach irrigation, and the traditional Chinese medicine composition is calculated according to the crude drug amount.
High dose tincture group: 3ml/100g of the traditional Chinese medicine composition of the example 1 is administrated by stomach irrigation, and the traditional Chinese medicine composition is calculated according to the crude drug amount.
The results show that: the traditional Chinese medicine tincture disclosed by the invention has no obvious influence on indexes such as hair, behaviors, stool and urine, weight, organ weight, hemogram, liver and kidney functions, blood sugar and blood fat of 7 groups of SD rats, and no abnormal change is found by naked eyes of organs and histological examination results show that after administration for 12 weeks and stopping administration for 4 weeks, all organs of the mice are not obviously changed. The traditional Chinese medicine tincture disclosed by the invention has small toxicity to mice after long-term administration, has no abnormal reaction after stopping administration, and is safe to apply.
Clinical effect test:
1. Case selection
The treatment cases are all kinds of muscle pain, bone injury and rheumatic joint pain patients, the ages are 23-68 years, the average age is 46 years, the course of the disease is 4 weeks to2 years, and most of the patients are treated by hospital examination and treatment, and the tincture is used for assisting treatment.
2. Application method
The traditional Chinese medicine tincture in the embodiment 1-5 is selected, 125 patients meeting the case standard are randomly divided into 5 groups for administration; selecting the traditional Chinese medicine tincture of the comparative examples 1-3, and randomly dividing 75 patients meeting the case standard into 3 groups for administration; spraying tincture onto external focus, wrapping with gauze, and absorbing by permeation. Spraying/applying once every 4-5 hours, and treating for 4 weeks. No more than 4 times daily, avoiding discomfort caused by overdosing.
3. Standard of efficacy
(1) And (3) curing: the symptoms disappear completely, and the leg and foot activities recover to be normal;
(2) Improvement: the main symptoms basically disappear, the activities of legs and feet are basically recovered to be normal, but pain is sometimes caused;
(3) Invalidation: compared with the prior treatment, the symptoms are unchanged.
4. Therapeutic results
The traditional Chinese medicine tincture disclosed by the invention is used for treating 125 typical cases, 32 cases are cured, 76 cases are effective, 12 cases are effective, 5 cases are ineffective, and the total effective rate is 96%; the traditional Chinese medicine tincture of comparative examples 1-3 is used, 75 cases are typical cases, 14 cases are cured, 37 cases are obvious in effect, 16 cases are effective, 8 cases are ineffective, and the total effective rate is 89.3%; no patient in the 8 example groups had adverse effects during the course of the trial. The medicine has the effects of astringing to stop bleeding, promoting blood circulation to remove blood stasis, relieving swelling and pain, promoting reunion of fractured bones, relaxing tendons and activating collaterals, dispelling wind and removing dampness, and can solve pain and quickly relieve swelling within 1-2 weeks. 1-2 treatment courses can promote muscle pain and bone injury repair, relieve rheumatic joint pain, improve mobility, and achieve treatment effect after 4-6 treatment courses.
5. Typical cases
1. Muscle pain cases:
The work of the king XX, female, 35 years old, sitting in front of a computer for a long time causes tension and pain of neck and shoulder muscles, and rest and simple massage cannot be effectively relieved. The doctor diagnoses the scapulohumeral periarthritis, and the disease course is half a year. Under the advice of doctors, the traditional Chinese medicine tincture provided by the invention is used, and contains traditional Chinese medicine extracts for relaxing tendons and activating blood. After the traditional Chinese medicine tincture is sprayed on the neck and shoulder muscles for 4 weeks, the pain of the neck and shoulder muscles is relieved, and after the traditional Chinese medicine tincture is continuously taken for 8 weeks, the muscle stiffness and pain symptoms are radically improved, and the normal work and life rhythm is restored.
2. Bone injury cases:
Liu XX, male, 23 years old, is an amateur football player, and is unfortunately injured in one match, resulting in fracture of the leg, and after normal treatment, the fracture part heals. However, during rehabilitation, he feels pain at the injured part, which affects rehabilitation training. The traditional Chinese medicine tincture is sprayed when receiving physical treatment. After the tincture is continuously used for 3 months, the symptoms of leg ache are obviously relieved, and normal training is restored.
3. Case of rheumatic arthralgia:
Zhao XX, female, 58 years old, retired teacher, had rheumatic arthritis for many years, especially in wet and cold weather, with severe joint pain, severely affecting daily life. Although various western medicines and rheumatism plasters were tried, only short relief was provided. Later, she tried a traditional Chinese medicine tincture, which has the main actions of activating blood, dispelling wind, warming meridians and relieving pain. The spray is adhered to every day and proper joint movement is matched. After 2 months Zhao Ayi found a significant relief of joint pain and a more flexible action. After the medicine is continuously taken for 6 months, the symptoms of the rheumatic arthritis are greatly relieved, and the life quality is obviously improved.
All of the above cases were performed under the direction of the doctor.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (7)
1. The production method of the traditional Chinese medicine tincture for activating blood and relaxing tendons is characterized by comprising the following steps of:
Step one, preparing a traditional Chinese medicine extract;
and i, weighing the traditional Chinese medicine components according to the proportion: ligusticum wallichii, root of red rooted saliva, cnidium fruit, kadsura pepper stem, cinnamon, licorice root, schizonepeta, bupleurum root and giant knotweed;
ii, rolling the traditional Chinese medicine components with good weight into coarse powder, and sieving with a 60-80 mesh sieve; adding appropriate amount of distilled water into the obtained Chinese medicinal powder to obtain suspension, and uniformly performing nanocrystallization grinding treatment in a liquid grinder to obtain Chinese medicinal emulsion;
Iii, pouring each Chinese medicinal material emulsion into a container containing ethanol solution for reflux extraction for 2-3 times, filtering out supernatant fluid each time, recovering ethanol, and concentrating filtrate at 75 ℃ and under the condition of minus 0.8 to minus 0.6MPa to obtain a Chinese medicinal extract containing active ingredients of each Chinese medicinal component for later use;
Step two, preparing tincture;
Weighing deer horn gelatin powder according to mass percent, mixing the deer horn gelatin powder with ethanol solution as a solvent, adding the prepared traditional Chinese medicine extract, and the gentiana macrophylla extract, the stiff silkworm extract, the mistletoe extract and the basil leaf extract which are weighed according to mass percent, uniformly mixing under the magnetic stirring effect with the stirring speed of 300-700 r/min, concentrating at the temperature of 75 ℃ to prepare the traditional Chinese medicine tincture with the alcohol content of 30-40 percent for promoting blood circulation and relaxing tendons;
the preparation method of the gentiana macrophylla extract comprises the following steps:
Grinding radix Gentianae Marcrophyllae into powder, filtering with 60-80 mesh sieve, adding distilled water 3 times of the volume to obtain radix Gentianae Marcrophyllae suspension, and grinding with liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-100 nm;
Ethanol is selected as an organic solvent, gentiana macrophylla powder and ethanol are mixed according to the mass to volume ratio of 1:5 to 1:10, leaching is carried out for 8 to 10 hours at the temperature of 60 to 80 ℃, and reflux extraction is carried out for 2 to 3 times; filtering the extract to remove solid residues, recovering ethanol to obtain a solution containing radix Gentianae Marcrophyllae component, concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain radix Gentianae Marcrophyllae extract;
The preparation method of the stiff silkworm extract comprises the following steps: grinding dried stiff silkworm into powder, adding 8 times of ethanol with the volume concentration of 75%, soaking for 10-15 minutes, heating in a water bath at 55-65 ℃ for 10-14 hours, reflux-extracting for 2-3 times, heating and evaporating to remove ethanol, stirring at 300-800rpm for 20-60 minutes under the water bath condition of 40-60 ℃ for 1/10-1/20 of the volume concentration, and sterilizing to obtain stiff silkworm extract;
The preparation method of the mistletoe extract comprises the following steps: pulverizing dried stem and leaf of herba Taxilli into coarse powder, placing into supercritical CO 2 extraction device, adding 5 times volume of ethanol as entrainer, extracting under 35-65MPa at 25-75deg.C to obtain herba Taxilli extract.
2. The method for producing a tincture of Chinese medicine for promoting blood circulation and relaxing tendons according to claim 1, wherein when the Chinese medicine powder obtained in the first step is prepared into suspension, distilled water which is 3-8 times of the mass of the Chinese medicine powder is added, and the mixture is uniformly subjected to nanocrystallization grinding in a liquid grinder at 25 ℃ until the particle size is 30-100nm.
3. The method for producing a blood-activating and tendon-relaxing Chinese medicinal tincture according to claim 2, wherein the Chinese medicinal extracts prepared in the first step comprise Ligusticum wallichii extract, salvia miltiorrhiza extract, fructus Cnidii extract, piper hancei extract, cinnamomum cassia extract, glycyrrhiza extract, schizonepeta tenuifolia extract, bupleurum chinense extract and Polygonum cuspidatum extract.
4. The method for producing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons according to claim 3, wherein the preparation method of the ligusticum wallichii extract is as follows:
Weighing a proper amount of ligusticum wallichii, rolling the ligusticum wallichii into coarse powder, sieving the coarse powder with a 60-80 mesh sieve, and adding 3 times of distilled water into the ligusticum wallichii powder to prepare suspension;
Performing nanocrystallization grinding treatment in a liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-70 nm;
Pouring the ligusticum chuanxiong hort emulsion into a container containing 75% ethanol solution for reflux extraction for 2-3 times, wherein each time period is 8-10 hours; filtering out supernatant, recovering ethanol, concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain rhizoma Ligustici Chuanxiong extract containing rhizoma Ligustici Chuanxiong effective components.
5. The method for producing a tincture for promoting blood circulation and relaxing muscles and tendons according to claim 4, wherein the filtrate is concentrated to 1/10-1/20 of the volume of the filtrate to obtain the Ligusticum chuanxiong extract containing the effective components of Ligusticum chuanxiong.
6. The method for producing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons according to claim 3, wherein the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons prepared in the second step comprises the following components in percentage by mass:
5% -9% of Ligusticum chuanxiong extract
5% -9% Of red sage root extract
3 To 7 percent of gentiana macrophylla extract
3 To 7 percent of cnidium fruit extract
3 To 5 percent of stiff silkworm extract
4% -6% Of mistletoe extract
3 To 7 percent of kadsura pepper stem extract
1 To 3 percent of deer horn gelatin powder
2% -6% Of cinnamon extract
1% -3% Of licorice extract
3 To 7 percent of schizonepeta extract
3 To 7 percent of bupleurum extract
3% -7% Of polygonum cuspidatum extract
2% -6% Of basil leaf extract
30% -40% Of ethanol.
7. The method for producing a Chinese medicinal tincture for promoting blood circulation and relaxing tendons according to claim 1, wherein the ratio of the weight of the medicinal material of the gentiana macrophylla powder to the volume of the ethanol is 1:5 to 1:10 when the gentiana macrophylla powder and the ethanol are mixed according to the ratio of mass to volume.
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