CN117731712A - Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons - Google Patents
Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons Download PDFInfo
- Publication number
- CN117731712A CN117731712A CN202410187266.5A CN202410187266A CN117731712A CN 117731712 A CN117731712 A CN 117731712A CN 202410187266 A CN202410187266 A CN 202410187266A CN 117731712 A CN117731712 A CN 117731712A
- Authority
- CN
- China
- Prior art keywords
- extract
- chinese medicine
- traditional chinese
- tincture
- powder
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000003814 drug Substances 0.000 title claims abstract description 108
- 229940098465 tincture Drugs 0.000 title claims abstract description 83
- 210000002435 tendon Anatomy 0.000 title claims abstract description 39
- 230000002040 relaxant effect Effects 0.000 title claims abstract description 38
- 230000017531 blood circulation Effects 0.000 title claims abstract description 36
- 230000001737 promoting effect Effects 0.000 title claims abstract description 32
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 27
- 239000000284 extract Substances 0.000 claims abstract description 178
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 106
- 239000000843 powder Substances 0.000 claims abstract description 52
- 241000501743 Gentiana macrophylla Species 0.000 claims abstract description 26
- 241000112528 Ligusticum striatum Species 0.000 claims abstract description 21
- 241000255789 Bombyx mori Species 0.000 claims abstract description 20
- 238000000605 extraction Methods 0.000 claims abstract description 19
- 108010010803 Gelatin Proteins 0.000 claims abstract description 16
- 229920000159 gelatin Polymers 0.000 claims abstract description 16
- 239000008273 gelatin Substances 0.000 claims abstract description 16
- 235000019322 gelatine Nutrition 0.000 claims abstract description 16
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 16
- 238000000227 grinding Methods 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 16
- 241000951473 Schizonepeta Species 0.000 claims abstract description 15
- 230000003213 activating effect Effects 0.000 claims abstract description 15
- 235000002566 Capsicum Nutrition 0.000 claims abstract description 14
- 241000282994 Cervidae Species 0.000 claims abstract description 14
- 239000006002 Pepper Substances 0.000 claims abstract description 14
- 235000016761 Piper aduncum Nutrition 0.000 claims abstract description 14
- 235000017804 Piper guineense Nutrition 0.000 claims abstract description 14
- 235000008184 Piper nigrum Nutrition 0.000 claims abstract description 14
- 238000003756 stirring Methods 0.000 claims abstract description 14
- 235000010676 Ocimum basilicum Nutrition 0.000 claims abstract description 13
- 240000007926 Ocimum gratissimum Species 0.000 claims abstract description 13
- 230000000694 effects Effects 0.000 claims abstract description 13
- 240000001341 Reynoutria japonica Species 0.000 claims abstract description 12
- 235000018167 Reynoutria japonica Nutrition 0.000 claims abstract description 12
- 239000008280 blood Substances 0.000 claims abstract description 12
- 210000004369 blood Anatomy 0.000 claims abstract description 12
- 239000000839 emulsion Substances 0.000 claims abstract description 12
- 239000000463 material Substances 0.000 claims abstract description 12
- 238000010992 reflux Methods 0.000 claims abstract description 12
- 238000001914 filtration Methods 0.000 claims abstract description 11
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 239000012153 distilled water Substances 0.000 claims abstract description 10
- 239000002904 solvent Substances 0.000 claims abstract description 9
- 235000014066 European mistletoe Nutrition 0.000 claims abstract description 8
- 235000006200 Glycyrrhiza glabra Nutrition 0.000 claims abstract description 8
- 244000152640 Rhipsalis cassutha Species 0.000 claims abstract description 8
- 235000012300 Rhipsalis cassutha Nutrition 0.000 claims abstract description 8
- 238000002156 mixing Methods 0.000 claims abstract description 8
- 239000000203 mixture Substances 0.000 claims abstract description 8
- 241000202726 Bupleurum Species 0.000 claims abstract description 7
- 239000004480 active ingredient Substances 0.000 claims abstract description 7
- 238000007709 nanocrystallization Methods 0.000 claims abstract description 6
- 244000223760 Cinnamomum zeylanicum Species 0.000 claims abstract description 5
- 235000017803 cinnamon Nutrition 0.000 claims abstract description 5
- 238000005096 rolling process Methods 0.000 claims abstract description 5
- 239000006228 supernatant Substances 0.000 claims abstract description 5
- 238000002360 preparation method Methods 0.000 claims description 14
- 241001506371 Kadsura Species 0.000 claims description 13
- 241000722363 Piper Species 0.000 claims description 13
- 241000212948 Cnidium Species 0.000 claims description 10
- 241000244365 Ligusticum sinense Species 0.000 claims description 10
- 240000007164 Salvia officinalis Species 0.000 claims description 10
- 235000020230 cinnamon extract Nutrition 0.000 claims description 10
- 235000013399 edible fruits Nutrition 0.000 claims description 10
- 239000000706 filtrate Substances 0.000 claims description 10
- 235000005412 red sage Nutrition 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 239000002245 particle Substances 0.000 claims description 7
- 230000001954 sterilising effect Effects 0.000 claims description 7
- 238000010438 heat treatment Methods 0.000 claims description 6
- 229940069445 licorice extract Drugs 0.000 claims description 6
- 238000005303 weighing Methods 0.000 claims description 6
- 244000153955 Reynoutria sachalinensis Species 0.000 claims description 4
- 235000003202 Reynoutria sachalinensis Nutrition 0.000 claims description 4
- 210000003205 muscle Anatomy 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 238000002791 soaking Methods 0.000 claims description 4
- 238000001704 evaporation Methods 0.000 claims description 3
- 238000002386 leaching Methods 0.000 claims description 3
- 239000003960 organic solvent Substances 0.000 claims description 3
- 239000007787 solid Substances 0.000 claims description 3
- 235000017443 Hedysarum boreale Nutrition 0.000 claims description 2
- 235000007858 Hedysarum occidentale Nutrition 0.000 claims description 2
- 239000012530 fluid Substances 0.000 claims description 2
- 239000001947 glycyrrhiza glabra rhizome/root Substances 0.000 claims description 2
- 238000003760 magnetic stirring Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 210000003296 saliva Anatomy 0.000 claims description 2
- 241000510654 Bupleurum chinense Species 0.000 claims 1
- 244000037364 Cinnamomum aromaticum Species 0.000 claims 1
- 235000014489 Cinnamomum aromaticum Nutrition 0.000 claims 1
- 235000021511 Cinnamomum cassia Nutrition 0.000 claims 1
- 241000202807 Glycyrrhiza Species 0.000 claims 1
- 241000244355 Ligusticum Species 0.000 claims 1
- 241000746609 Piper hancei Species 0.000 claims 1
- 235000011135 Salvia miltiorrhiza Nutrition 0.000 claims 1
- 241000951376 Schizonepeta tenuifolia Species 0.000 claims 1
- LTINPJMVDKPJJI-UHFFFAOYSA-N iodinated glycerol Chemical compound CC(I)C1OCC(CO)O1 LTINPJMVDKPJJI-UHFFFAOYSA-N 0.000 claims 1
- 244000132619 red sage Species 0.000 claims 1
- 244000303040 Glycyrrhiza glabra Species 0.000 abstract description 7
- LPLVUJXQOOQHMX-QWBHMCJMSA-N glycyrrhizinic acid Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@H](O[C@@H]1O[C@@H]1C([C@H]2[C@]([C@@H]3[C@@]([C@@]4(CC[C@@]5(C)CC[C@@](C)(C[C@H]5C4=CC3=O)C(O)=O)C)(C)CC2)(C)CC1)(C)C)C(O)=O)[C@@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O LPLVUJXQOOQHMX-QWBHMCJMSA-N 0.000 abstract description 6
- 235000011477 liquorice Nutrition 0.000 abstract description 6
- 241001506304 Kadsura japonica Species 0.000 abstract 1
- 244000203593 Piper nigrum Species 0.000 abstract 1
- 230000000052 comparative effect Effects 0.000 description 18
- 239000000243 solution Substances 0.000 description 10
- 230000036407 pain Effects 0.000 description 9
- 208000002193 Pain Diseases 0.000 description 8
- 208000000112 Myalgia Diseases 0.000 description 7
- 229940079593 drug Drugs 0.000 description 7
- 230000002262 irrigation Effects 0.000 description 7
- 238000003973 irrigation Methods 0.000 description 7
- 208000013465 muscle pain Diseases 0.000 description 7
- 210000002784 stomach Anatomy 0.000 description 7
- 208000024891 symptom Diseases 0.000 description 7
- 206010061363 Skeletal injury Diseases 0.000 description 6
- 241000316342 Taxillus Species 0.000 description 6
- 208000006820 Arthralgia Diseases 0.000 description 5
- 239000009849 Bupleurum root extract Substances 0.000 description 5
- 239000002994 raw material Substances 0.000 description 5
- 208000025747 Rheumatic disease Diseases 0.000 description 4
- 201000010099 disease Diseases 0.000 description 4
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 4
- 238000007689 inspection Methods 0.000 description 4
- 238000004806 packaging method and process Methods 0.000 description 4
- 230000000552 rheumatic effect Effects 0.000 description 4
- 241000699670 Mus sp. Species 0.000 description 3
- 230000037396 body weight Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 241000255791 Bombyx Species 0.000 description 2
- 241000700159 Rattus Species 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 230000002354 daily effect Effects 0.000 description 2
- 230000000857 drug effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 201000003068 rheumatic fever Diseases 0.000 description 2
- 238000005507 spraying Methods 0.000 description 2
- 230000008961 swelling Effects 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- 241000212322 Levisticum officinale Species 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 206010052904 Musculoskeletal stiffness Diseases 0.000 description 1
- 206010034464 Periarthritis Diseases 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 210000003056 antler Anatomy 0.000 description 1
- 230000006399 behavior Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000740 bleeding effect Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007012 clinical effect Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000009975 flexible effect Effects 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 238000003304 gavage Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 238000010562 histological examination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 239000001645 levisticum officinale Substances 0.000 description 1
- 239000012669 liquid formulation Substances 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000002674 ointment Substances 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 239000006187 pill Substances 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000033764 rhythmic process Effects 0.000 description 1
- 208000018320 severe joint pain Diseases 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 239000003826 tablet Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 231100000820 toxicity test Toxicity 0.000 description 1
- 229940126680 traditional chinese medicines Drugs 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 210000003462 vein Anatomy 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 229940126673 western medicines Drugs 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention relates to the field of traditional Chinese medicine, and provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which comprises the steps of rolling Ligusticum wallichii, radix salviae miltiorrhizae, fructus cnidii, kadsura pepper stem, cinnamon, liquorice, schizonepeta, bupleurum and polygonum cuspidatum into coarse powder, mixing with distilled water to prepare suspension, carrying out nanocrystallization grinding to obtain emulsion of traditional Chinese medicine materials, mixing with ethanol solution, carrying out reflux extraction, filtering and recovering ethanol from the supernatant after extraction, and concentrating to obtain an extract containing active ingredients of each traditional Chinese medicine component; mixing deer horn gelatin powder with ethanol solution as a solvent base, adding the traditional Chinese medicine extract prepared in the previous step into the solvent, adding the gentiana macrophylla extract, the stiff silkworm extract, the mistletoe extract and the basil leaf extract which are weighed according to mass percent into the mixture, stirring and concentrating until the alcohol content is reached, and finally preparing the traditional Chinese medicine tincture with the effects of activating blood and relaxing tendons, wherein the maximum extraction of active ingredients can be ensured, and the double effects of activating blood circulation and dissolving stasis and relaxing tendons and activating collaterals are considered.
Description
Technical Field
The invention relates to the field of traditional Chinese medicines, in particular to a method for producing a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons.
Background
In the theory of traditional Chinese medicine, many diseases are related to unsmooth blood circulation and blocked tendons and veins. The Chinese medicine has the functions of promoting blood circulation to disperse blood clots, relaxing tendons and activating collaterals, and is one important Chinese medicine method for treating muscle pain, bone injury, rheumatic arthralgia and other symptoms. The traditional Chinese medicine has the advantages of utilizing the special efficacy of the natural medicinal materials, achieving the purposes of activating blood circulation to dissipate blood stasis and relaxing tendons and activating collaterals through various complex pharmacological actions, and treating various diseases caused by unsmooth qi and blood circulation.
The traditional Chinese medicine tincture mainly takes the forms of pills, tablets, ointment, powder and the like, and the preparations have wide clinical application. However, these traditional Chinese medicinal tinctures generally have the problems of long acting time, inconvenient carrying, uneven drug effect exertion, insufficient penetrating power and the like. In modern society, people are more and more pursuing therapeutic effects, and meanwhile, convenient and quick administration is also expected.
Tincture is favored as a liquid formulation for its rapid absorption rate and good penetration. The tincture is prepared by soaking Chinese medicinal materials in ethanol or ethanol solution to dissolve the effective components in the Chinese medicinal materials in solvent, and is administered orally or topically. Because ethanol has good dissolving capacity and high absorption speed, the tincture can play a role rapidly and is convenient to store and carry.
Despite the advantages, the tincture has the following defects: the formula of part of the products is single, and the drug effect is not comprehensive enough; the production process is not scientific enough, so that the extraction of the effective components is incomplete, and the like. Therefore, the development of a novel blood-activating and tendon-relaxing traditional Chinese medicine tincture and a scientific and reasonable production method thereof have important significance for meeting the health requirements of modern people.
Disclosure of Invention
The invention aims to provide a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by adopting a plurality of medicinal material compounds, has the double effects of promoting blood circulation and removing blood stasis and relaxing tendons and activating collaterals, optimizes the production process, ensures the maximum extraction of active ingredients, achieves the aim of quickly and effectively treating muscle pain and bone injury, and is convenient to carry and use.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a method for producing a traditional Chinese medicine tincture for activating blood and relaxing tendons comprises the following steps:
step one, preparing a traditional Chinese medicine extract;
and i, weighing the traditional Chinese medicine components according to the proportion: ligusticum wallichii, root of red rooted saliva, cnidium fruit, kadsura pepper stem, cinnamon, licorice root, schizonepeta, bupleurum root and giant knotweed;
ii, rolling the traditional Chinese medicine components with good weight into coarse powder, and sieving with a 60-80 mesh sieve; adding appropriate amount of distilled water into the obtained Chinese medicinal powder to obtain suspension, and uniformly performing nanocrystallization grinding treatment in a liquid grinder to obtain Chinese medicinal emulsion;
iii, pouring each Chinese medicinal material emulsion into a container containing ethanol solution for reflux extraction for 2-3 times, filtering out supernatant fluid each time, recovering ethanol, and concentrating filtrate at 75 ℃ and under the condition of minus 0.8 to minus 0.6MPa to obtain a Chinese medicinal extract containing active ingredients of each Chinese medicinal component for later use;
step two, preparing tincture;
the preparation method comprises the steps of weighing deer horn gelatin powder according to mass percentage, mixing the deer horn gelatin powder with ethanol solution to be used as a solvent, adding the prepared traditional Chinese medicine extract, and the gentiana macrophylla extract, the stiff silkworm extract, the mistletoe extract and the basil leaf extract which are weighed according to mass percentage, uniformly mixing under the magnetic stirring effect of stirring speed of 300-700 r/min, concentrating at 75 ℃, and preparing the traditional Chinese medicine tincture with 30-40% of alcohol content for promoting blood circulation and relaxing tendons.
As a further scheme of the invention, when each traditional Chinese medicine powder obtained in the step one is prepared into suspension, distilled water which is 3-8 times of the mass of each traditional Chinese medicine powder is added, and nano grinding is uniformly carried out in a liquid grinder at the temperature of 25 ℃ until the particle size is 30-100nm.
As a further scheme of the invention, the traditional Chinese medicine extract prepared in the first step comprises a ligusticum wallichii extract, a radix salviae miltiorrhizae extract, a fructus cnidii extract, a kadsura pepper stem extract, a cinnamon extract, a liquorice extract, a schizonepeta extract, a radix bupleuri extract and a polygonum cuspidatum extract.
As a further scheme of the invention, the preparation method of the ligusticum wallichii extract comprises the following steps:
weighing a proper amount of ligusticum wallichii, rolling the ligusticum wallichii into coarse powder, sieving the coarse powder with a 60-80 mesh sieve, and adding 3 times of distilled water into the ligusticum wallichii powder to prepare suspension;
performing nanocrystallization grinding treatment in a liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-70 nm;
pouring the ligusticum chuanxiong hort emulsion into a container containing 75% ethanol solution for reflux extraction for 2-3 times, wherein each time period is 8-10 hours; filtering out supernatant, recovering ethanol, concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain rhizoma Ligustici Chuanxiong extract containing rhizoma Ligustici Chuanxiong effective components.
As a further scheme of the invention, when the ligusticum chuanxiong hort extract is prepared, the volume of the filtrate is concentrated to 1/10-1/20, and the ligusticum chuanxiong hort extract containing the effective components of the ligusticum chuanxiong hort is obtained.
As a further scheme of the invention, the blood-activating and tendon-relaxing traditional Chinese medicine tincture prepared in the second step comprises the following components in percentage by mass:
5% -9% of Ligusticum chuanxiong extract
5% -9% of red sage root extract
3 to 7 percent of gentiana macrophylla extract
3 to 7 percent of cnidium fruit extract
3 to 5 percent of stiff silkworm extract
4% -6% of mistletoe extract
3 to 7 percent of kadsura pepper stem extract
1 to 3 percent of deer horn gelatin powder
2% -6% of cinnamon extract
1% -3% of licorice extract
3 to 7 percent of schizonepeta extract
3 to 7 percent of bupleurum extract
3% -7% of polygonum cuspidatum extract
2% -6% of basil leaf extract
30% -40% of ethanol.
As a further scheme of the invention, the preparation method of the gentiana macrophylla extract comprises the following steps:
grinding radix Gentianae Marcrophyllae into powder, filtering with 60-80 mesh sieve, adding distilled water 3 times of the volume to obtain radix Gentianae Marcrophyllae suspension, and grinding with liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-100 nm;
ethanol is selected as an organic solvent, gentiana macrophylla powder and ethanol are mixed according to the proportion of 1:5 to 1:10, leaching is carried out for 8 to 10 hours at the temperature of 60 to 80 ℃, and reflux extraction is carried out for 2 to 3 times; filtering the extractive solution to remove solid residue, recovering ethanol to obtain solution containing radix Gentianae Marcrophyllae component, and concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain radix Gentianae Marcrophyllae extract.
As a further scheme of the invention, when the gentiana macrophylla powder and the ethanol are mixed according to the proportion of 1:5 to 1:10, the solvent volume ratio of the medicine weight of the gentiana macrophylla powder to the ethanol is adopted.
As a further scheme of the invention, the preparation method of the stiff silkworm extract comprises the following steps: grinding dried stiff silkworm into powder, adding 8 times of ethanol with the volume concentration of 75%, soaking for 10-15 minutes, heating in a water bath at 55-65 ℃ for 10-14 hours, reflux extracting for 2-3 times, heating and evaporating to remove ethanol, stirring at 300-800rpm for 20-60 minutes under the water bath condition of 40-60 ℃ for 1/10-1/20 of the volume concentration, and sterilizing to obtain the stiff silkworm extract.
As a further scheme of the invention, the preparation method of the mistletoe extract comprises the following steps: pulverizing dried stem and leaf of herba Taxilli into coarse powder, and placing into supercritical CO 2 In the extraction apparatusAdding 5 times volume of ethanol as entrainer, extracting under 35-65MPa at 25-75deg.C to obtain herba Taxilli extract.
Compared with the prior art, the invention has the beneficial effects that:
according to the production method of the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is provided by the invention, extracts of a plurality of traditional Chinese medicinal materials such as ligusticum wallichii, radix salviae miltiorrhizae, radix gentianae macrophyllae, fructus cnidii, stiff silkworm, herba taxilli, caulis kadsurae, cinnamon, liquorice, schizonepeta, radix bupleuri, polygonum cuspidatum and the like are combined with the deer antler gelatin powder and the basil leaf extract through ethanol reflux extraction and concentration, so that the tincture with the effects of promoting blood circulation and removing blood stasis, relaxing tendons and activating collaterals and diminishing inflammation and relieving pain can be produced, the effective components can be effectively extracted from traditional Chinese medicine materials, meanwhile, the effective components in the extract are not destroyed through controlling the pressure and the temperature, the concentration of the active components in the extract can be ensured, the final tincture has strong efficacy, the alcohol content of the prepared tincture is 30% -40%, the stability of the effective components can be ensured, the pollution of external microorganisms can be reduced, and the effective period of the medicine can be prolonged.
Detailed Description
The technical scheme of the invention is further described in detail below with reference to the specific embodiments.
Aiming at the problems of single prescription, incomplete efficacy and incomplete extraction of active ingredients caused by insufficient scientific production process of the blood-activating and tendon-relaxing tincture products on the market, the invention aims to develop a novel production method of the blood-activating and tendon-relaxing traditional Chinese medicine tincture, the blood-activating and tendon-relaxing traditional Chinese medicine tincture prepared by the method is prepared by adopting a plurality of medicinal material compounds, has double effects of activating blood circulation to dissipate blood stasis and relaxing tendons and activating collaterals, optimizes the production process, ensures the maximum extraction of the active ingredients, and simultaneously achieves the aims of rapidly and effectively treating muscle pain and bone injury, and is convenient to carry and use.
The application provides a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which comprises the following components in percentage by mass: 5-9% of ligusticum wallichii extract, 5-9% of red sage root extract, 3-7% of gentiana macrophylla extract, 3-7% of fructus cnidii extract, 3-5% of stiff silkworm extract, 4-6% of Chinese taxillus twig extract, 3-7% of kadsura pepper stem extract, 1-3% of deer horn gelatin powder, 2-6% of cinnamon extract, 1-3% of liquorice extract, 3-7% of schizonepeta extract, 3-7% of radix bupleuri extract, 3-7% of polygonum cuspidatum extract, 2-6% of basil leaf extract and 30-40% of ethanol.
In the application, if not specified, the gentiana macrophylla extract is prepared by grinding the gentiana macrophylla into powder, filtering the powder through a 80-mesh sieve, adding distilled water with 3 times of volume to prepare a gentiana macrophylla suspension, and grinding the suspension through a liquid grinder to obtain ligusticum chuanxiong hort emulsion with the particle size of 30-100 nm; ethanol is selected as an organic solvent, gentiana macrophylla powder and ethanol are mixed according to the proportion of 1:5, leaching is carried out for 10 hours at 60 ℃, and reflux extraction is carried out for 3 times; filtering the extractive solution to remove solid residue, recovering ethanol to obtain solution containing radix Gentianae Marcrophyllae component, concentrating the filtrate at 75deg.C and-0.6 MPa to obtain radix Gentianae Marcrophyllae extract.
Wherein, when the gentiana macrophylla powder and the ethanol are mixed according to the proportion of 1:5, the solvent volume ratio of the weight of the medicinal material of the gentiana macrophylla powder to the ethanol is adopted.
According to the steps, the traditional Chinese medicine components of the red sage root, the cnidium fruit, the kadsura pepper stem, the cinnamon, the liquorice, the schizonepeta, the bupleurum and the giant knotweed are respectively extracted to obtain the traditional Chinese medicine extract containing the effective components of the traditional Chinese medicine components: ligusticum wallichii extract, red sage root extract, cnidium fruit extract, kadsura pepper stem extract, cinnamon extract, licorice extract, schizonepeta extract, bupleurum extract and polygonum cuspidatum extract.
In the application, the stiff silkworm extract is prepared by grinding the stiff silkworm obtained into powder, adding ethanol with the volume concentration of 75% in an amount which is 8 times that of the powder, soaking for 15 minutes, heating in a water bath at 55 ℃ for 14 hours, extracting under reflux for 3 times, heating and evaporating to remove the ethanol, carrying out volume concentration to 1/10 in a water bath at 60 ℃ with the stirring speed of 350rpm for 50 minutes, and sterilizing.
In the application, when herba Taxilli extract is prepared, dry stem and leaf of herba Taxilli is taken as raw material, crushed into coarse powder, and placed in supercritical CO 2 In the extraction device, ethanol with the volume of 5 times is added as entrainer, the extraction pressure is 50MPa, the temperature is 45 ℃, and the mistletoe extract is obtained.
The following will explain in conjunction with specific examples 1 to 5.
Wherein, each component of the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons of the example 1 to the example 5 and the dosage of each component are shown in the table 1 below, wherein, each component dosage in each tincture is g.
Table 1 examples 1-5 Each component and the amount (g) of each component of the blood-activating and tendon-relaxing Chinese medicinal tincture
Specific examples and preparation methods are as follows:
example 1
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
step 1), putting 2g of deer horn gelatin powder and 40 g of ethanol into a medicine tank, stirring uniformly, and adding 7g of prepared ligusticum wallichii extract, 7g of red sage root extract, 5g of gentiana macrophylla extract, 5g of cnidium fruit extract, 4g of stiff silkworm extract, 5g of Chinese taxillus herb extract, 5g of kadsura pepper stem extract, 4g of cinnamon extract, 2g of licorice extract, 5g of schizonepeta extract, 5g of bupleurum root extract, 5g of polygonum cuspidatum extract and 4g of basil leaf extract.
Step 2), uniformly stirring at a speed of 350r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 35%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Example 2
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
step 1), putting 3g of deer horn gelatin powder and 35 g of ethanol into a medicine tank, stirring uniformly, and adding 9g of prepared ligusticum wallichii extract, 5g of red sage root extract, 7g of gentiana macrophylla extract, 4g of cnidium fruit extract, 5g of stiff silkworm extract, 6g of Chinese taxillus herb extract, 7g of kadsura pepper stem extract, 2g of cinnamon extract, 3g of licorice extract, 3g of schizonepeta extract, 6g of bupleurum root extract, 5g of polygonum cuspidatum extract and 5g of basil leaf extract.
Step 2), uniformly stirring at a speed of 300r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 30%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Example 3
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
step 1), putting 2g of deer horn gelatin powder and 45 g of ethanol into a medicine tank, stirring uniformly, and adding 5g of prepared ligusticum wallichii extract, 5g of red sage root extract, 5g of gentiana macrophylla extract, 5g of cnidium fruit extract, 3g of stiff silkworm extract, 5g of Chinese taxillus herb extract, 5g of kadsura pepper stem extract, 5g of cinnamon extract, 2g of licorice extract, 3g of schizonepeta extract, 5g of bupleurum root extract, 5g of polygonum cuspidatum extract and 5g of basil leaf extract.
Step 2), uniformly stirring at the speed of 700r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 40%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Example 4
The difference from example 2 is that: 1g of deer horn gelatin powder, 8g of szechuan lovage rhizome extract, 7g of red sage root extract, 7g of common cnidium fruit extract, 5g of Chinese taxillus herb extract, 5g of kadsura pepper stem extract, 3g of cinnamon extract, 1g of liquoric root extract, 5g of schizonepeta extract, 7g of bupleurum root extract, 3g of giant knotweed rhizome extract and 6g of basil leaf extract.
Example 5
The embodiment of the invention provides a production method of a traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons, which is prepared by the following steps:
step 1), putting 1g of deer horn gelatin powder and 41 g of ethanol into a medicine tank, stirring uniformly, and adding 9g of prepared ligusticum wallichii extract, 9g of red sage root extract, 3g of gentiana macrophylla extract, 3g of fructus cnidii extract, 4g of stiff silkworm extract, 4g of Chinese taxillus twig extract, 3g of kadsura pepper stem extract, 6g of cinnamon extract, 3g of liquorice extract, 7g of schizonepeta extract, 3g of bupleurum root extract, 7g of polygonum cuspidatum extract and 2g of basil leaf extract.
Step 2), uniformly stirring at a speed of 650r/min under a magnetic stirrer, concentrating at 75 ℃ until the alcohol content is about 36%, sterilizing the obtained blood-activating and tendon-relaxing traditional Chinese medicine tincture, and packaging after quality inspection.
Based on the above examples 1 to 5, referring to the amounts of the components shown in table 1, the rhizoma Ligustici Chuanxiong extract, saviae Miltiorrhizae radix extract, radix Gentianae Marcrophyllae extract, fructus Cnidii extract, bombyx Batryticatus extract, herba Taxilli extract and caulis Piperis Futokadsurae extract etc. required for preparing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons are weighed, when preparing, the deer-horn gelatin powder is weighed according to the mass percentage, the deer-horn gelatin powder is mixed with ethanol solution as a solvent, and the prepared traditional Chinese medicine extract, the radix Gentianae Marcrophyllae extract, the Bombyx Batryticatus extract, the herba Taxilli extract and the herba Ocimi leaf extract are added, and concentrated after mixing uniformly, the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons is prepared.
Comparative example
According to the difference from example 1, the respective weights of the ligusticum wallichii extract, the radix salviae miltiorrhizae extract and the gentiana macrophylla extract are different, and a blood-activating and tendon-relaxing traditional Chinese medicine tincture of comparative examples 1 to 3 is prepared, wherein the respective raw materials and the respective raw material dosages of the blood-activating and tendon-relaxing traditional Chinese medicine tincture of comparative examples 1 to 3 are shown in the following table 2, and the respective raw material dosages are g.
Table 2 comparative examples 1-3 various raw materials and the dosage (g) of the Chinese medicinal tincture for promoting blood circulation and relaxing tendons
Specific examples and preparation methods are as follows:
referring to the components shown in Table 2 and the amounts of the components, the components required for preparing the blood-activating and tendon-relaxing traditional Chinese medicine tincture are weighed, and the difference between comparative example 1 and example 1 is that: the prepared traditional Chinese medicine tincture does not contain gentiana macrophylla extract, and the comparison example 2 is different from the example 1 in that: the prepared Chinese medicinal tincture does not contain stiff silkworm extract, and comparative example 3 is different from example 1 in that: the prepared traditional Chinese medicine tincture does not contain the basil leaf extract, and the preparation method is the same as that of the example 1, so as to produce the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons.
Toxicity experiment: the Chinese medicinal tinctures prepared in example 1 and comparative examples 1 to 3 of the present invention were continuously administered to mice for 12 weeks and stopped for 4 weeks, wherein SD rats were randomly divided into 7 groups of 10 animals each, and named: blank, comparative example 1, comparative example 2, comparative example 3, example 1, experimental, low, medium, high dose tincture. Specific:
blank control group: the same volume of physiological saline was administered by gavage.
Comparative example 1 control group: the same volume of the traditional Chinese medicine tincture of the comparative example 1 is administrated by stomach irrigation, and the traditional Chinese medicine tincture is calculated according to the crude drug amount of 1.0ml/100g of body weight for 2 times per day.
Comparative example 2 control group: the same volume of the traditional Chinese medicine tincture of the comparative example 2 is administrated by stomach irrigation, and the traditional Chinese medicine tincture is calculated according to the crude drug amount of 1.0ml/100g body weight for 2 times per day.
Comparative example 3 control group: the same volume of the traditional Chinese medicine tincture of the comparative example 3 is administrated by stomach irrigation, and the traditional Chinese medicine tincture is calculated according to the crude drug amount of 1.0ml/100g body weight for 2 times per day.
Example 1 experimental group: the same volume of the traditional Chinese medicine tincture of the example 1 is administrated by stomach irrigation, wherein the weight of the traditional Chinese medicine tincture is 1.0ml/100g, and the crude drug amount is 2 times per day.
Low dose tincture group: the traditional Chinese medicine composition of the example 1 is administrated by stomach irrigation at a concentration of 0.5ml/100g, and the traditional Chinese medicine composition is calculated according to the crude drug amount.
Group of medium dose tinctures: 1.2ml/100g of the traditional Chinese medicine composition of the example 1 is administrated by stomach irrigation, and the traditional Chinese medicine composition is calculated according to the crude drug amount.
High dose tincture group: 3ml/100g of the traditional Chinese medicine composition of the example 1 is administrated by stomach irrigation, and the traditional Chinese medicine composition is calculated according to the crude drug amount.
The results show that: the traditional Chinese medicine tincture disclosed by the invention has no obvious influence on indexes such as hair, behaviors, stool and urine, weight, organ weight, hemogram, liver and kidney functions, blood sugar and blood fat of 7 groups of SD rats, and no abnormal change is found by naked eyes of organs and histological examination results show that after administration for 12 weeks and stopping administration for 4 weeks, all organs of the mice are not obviously changed. The traditional Chinese medicine tincture disclosed by the invention has small toxicity to mice after long-term administration, has no abnormal reaction after stopping administration, and is safe to apply.
Clinical effect test:
1. case selection
The treatment cases are all kinds of muscle pain, bone injury and rheumatic joint pain patients, the ages are 23-68 years, the average age is 46 years, the course of the disease is 4 weeks to 2 years, and most of the patients are treated by hospital examination and treatment, and the tincture is used for assisting treatment.
2. Application method
The traditional Chinese medicine tincture in the embodiment 1-5 is selected, 125 patients meeting the case standard are randomly divided into 5 groups for administration; selecting the traditional Chinese medicine tincture of the comparative examples 1-3, and randomly dividing 75 patients meeting the case standard into 3 groups for administration; spraying tincture onto external focus, wrapping with gauze, and absorbing by permeation. Spraying/applying once every 4-5 hours, and treating for 4 weeks. No more than 4 times daily, avoiding discomfort caused by overdosing.
3. Standard of efficacy
(1) And (3) curing: the symptoms disappear completely, and the leg and foot activities recover to be normal;
(2) Improvement: the main symptoms basically disappear, the activities of legs and feet are basically recovered to be normal, but pain is sometimes caused;
(3) Invalidation: compared with the prior treatment, the symptoms are unchanged.
4. Therapeutic results
The traditional Chinese medicine tincture disclosed by the invention is used for treating 125 typical cases, 32 cases are cured, 76 cases are effective, 12 cases are effective, 5 cases are ineffective, and the total effective rate is 96%; the traditional Chinese medicine tincture of comparative examples 1-3 is used, 75 cases are typical cases, 14 cases are cured, 37 cases are obvious in effect, 16 cases are effective, 8 cases are ineffective, and the total effective rate is 89.3%; no patient in the 8 example groups had adverse effects during the course of the trial. The medicine has the effects of astringing to stop bleeding, promoting blood circulation to remove blood stasis, relieving swelling and pain, promoting reunion of fractured bones, relaxing tendons and activating collaterals, dispelling wind and removing dampness, and can solve pain and quickly relieve swelling within 1-2 weeks. 1-2 treatment courses can promote muscle pain and bone injury repair, relieve rheumatic joint pain, improve mobility, and achieve treatment effect after 4-6 treatment courses.
5. Typical cases
1. Muscle pain cases:
the work of the king XX, female, 35 years old, sitting in front of a computer for a long time causes tension and pain of neck and shoulder muscles, and rest and simple massage cannot be effectively relieved. The doctor diagnoses the scapulohumeral periarthritis, and the disease course is half a year. Under the advice of doctors, the traditional Chinese medicine tincture provided by the invention is used, and contains traditional Chinese medicine extracts for relaxing tendons and activating blood. After the traditional Chinese medicine tincture is sprayed on the neck and shoulder muscles for 4 weeks, the pain of the neck and shoulder muscles is relieved, and after the traditional Chinese medicine tincture is continuously taken for 8 weeks, the muscle stiffness and pain symptoms are radically improved, and the normal work and life rhythm is restored.
2. Bone injury cases:
liu XX, male, 23 years old, is an amateur football player, and is unfortunately injured in one match, resulting in fracture of the leg, and after normal treatment, the fracture part heals. However, during rehabilitation, he feels pain at the injured part, which affects rehabilitation training. The traditional Chinese medicine tincture is sprayed when receiving physical treatment. After the tincture is continuously used for 3 months, the symptoms of leg ache are obviously relieved, and normal training is restored.
3. Case of rheumatic arthralgia:
zhao XX, female, 58 years old, retired teacher, had rheumatic arthritis for many years, especially in wet and cold weather, with severe joint pain, severely affecting daily life. Although various western medicines and rheumatism plasters were tried, only short relief was provided. Later, she tried a traditional Chinese medicine tincture, which has the main actions of activating blood, dispelling wind, warming meridians and relieving pain. The spray is adhered to every day and proper joint movement is matched. After 2 months Zhao Ayi found a significant relief of joint pain and a more flexible action. After the medicine is continuously taken for 6 months, the symptoms of the rheumatic arthritis are greatly relieved, and the life quality is obviously improved.
All of the above cases were performed under the direction of the doctor.
While the preferred embodiments of the present invention have been described in detail, the present invention is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present invention within the knowledge of those skilled in the art.
Claims (10)
1. The production method of the traditional Chinese medicine tincture for activating blood and relaxing tendons is characterized by comprising the following steps of:
step one, preparing a traditional Chinese medicine extract;
and i, weighing the traditional Chinese medicine components according to the proportion: ligusticum wallichii, root of red rooted saliva, cnidium fruit, kadsura pepper stem, cinnamon, licorice root, schizonepeta, bupleurum root and giant knotweed;
ii, rolling the traditional Chinese medicine components with good weight into coarse powder, and sieving with a 60-80 mesh sieve; adding appropriate amount of distilled water into the obtained Chinese medicinal powder to obtain suspension, and uniformly performing nanocrystallization grinding treatment in a liquid grinder to obtain Chinese medicinal emulsion;
iii, pouring each Chinese medicinal material emulsion into a container containing ethanol solution for reflux extraction for 2-3 times, filtering out supernatant fluid each time, recovering ethanol, and concentrating filtrate at 75 ℃ and under the condition of minus 0.8 to minus 0.6MPa to obtain a Chinese medicinal extract containing active ingredients of each Chinese medicinal component for later use;
step two, preparing tincture;
the preparation method comprises the steps of weighing deer horn gelatin powder according to mass percentage, mixing the deer horn gelatin powder with ethanol solution to be used as a solvent, adding the prepared traditional Chinese medicine extract, and the gentiana macrophylla extract, the stiff silkworm extract, the mistletoe extract and the basil leaf extract which are weighed according to mass percentage, uniformly mixing under the magnetic stirring effect of stirring speed of 300-700 r/min, concentrating at 75 ℃, and preparing the traditional Chinese medicine tincture with 30-40% of alcohol content for promoting blood circulation and relaxing tendons.
2. The method for producing a tincture of Chinese medicine for promoting blood circulation and relaxing tendons according to claim 1, wherein when the Chinese medicine powder obtained in the first step is prepared into suspension, distilled water which is 3-8 times of the mass of the Chinese medicine powder is added, and the mixture is uniformly subjected to nanocrystallization grinding in a liquid grinder at 25 ℃ until the particle size is 30-100nm.
3. The method for producing a blood-activating and tendon-relaxing Chinese medicinal tincture according to claim 2, wherein the Chinese medicinal extracts prepared in the first step comprise Ligusticum wallichii extract, salvia miltiorrhiza extract, fructus Cnidii extract, piper hancei extract, cinnamomum cassia extract, glycyrrhiza extract, schizonepeta tenuifolia extract, bupleurum chinense extract and Polygonum cuspidatum extract.
4. The method for producing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons according to claim 3, wherein the preparation method of the ligusticum wallichii extract is as follows:
weighing a proper amount of ligusticum wallichii, rolling the ligusticum wallichii into coarse powder, sieving the coarse powder with a 60-80 mesh sieve, and adding 3 times of distilled water into the ligusticum wallichii powder to prepare suspension;
performing nanocrystallization grinding treatment in a liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-70 nm;
pouring the ligusticum chuanxiong hort emulsion into a container containing 75% ethanol solution for reflux extraction for 2-3 times, wherein each time period is 8-10 hours; filtering out supernatant, recovering ethanol, concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain rhizoma Ligustici Chuanxiong extract containing rhizoma Ligustici Chuanxiong effective components.
5. The method for producing a tincture for promoting blood circulation and relaxing muscles and tendons according to claim 4, wherein the filtrate is concentrated to 1/10-1/20 of the volume of the filtrate to obtain the Ligusticum chuanxiong extract containing the effective components of Ligusticum chuanxiong.
6. The method for producing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons according to claim 3, wherein the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons prepared in the second step comprises the following components in percentage by mass:
5% -9% of Ligusticum chuanxiong extract
5% -9% of red sage root extract
3 to 7 percent of gentiana macrophylla extract
3 to 7 percent of cnidium fruit extract
3 to 5 percent of stiff silkworm extract
4% -6% of mistletoe extract
3 to 7 percent of kadsura pepper stem extract
1 to 3 percent of deer horn gelatin powder
2% -6% of cinnamon extract
1% -3% of licorice extract
3 to 7 percent of schizonepeta extract
3 to 7 percent of bupleurum extract
3% -7% of polygonum cuspidatum extract
2% -6% of basil leaf extract
30% -40% of ethanol.
7. The method for producing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons according to claim 6, wherein the preparation method of the gentiana macrophylla extract is as follows:
grinding radix Gentianae Marcrophyllae into powder, filtering with 60-80 mesh sieve, adding distilled water 3 times of the volume to obtain radix Gentianae Marcrophyllae suspension, and grinding with liquid grinder to obtain rhizoma Ligustici Chuanxiong emulsion with particle diameter of 30-100 nm;
ethanol is selected as an organic solvent, gentiana macrophylla powder and ethanol are mixed according to the proportion of 1:5 to 1:10, leaching is carried out for 8 to 10 hours at the temperature of 60 to 80 ℃, and reflux extraction is carried out for 2 to 3 times; filtering the extractive solution to remove solid residue, recovering ethanol to obtain solution containing radix Gentianae Marcrophyllae component, and concentrating the filtrate at 75deg.C under-0.8 to-0.6 MPa to obtain radix Gentianae Marcrophyllae extract.
8. The method for producing a Chinese medicinal tincture for promoting blood circulation and relaxing tendons according to claim 7, wherein the ratio of the weight of the medicinal material of the gentiana macrophylla powder to the volume of the solvent of the ethanol is 1:5 to 1:10 when the gentiana macrophylla powder and the ethanol are mixed.
9. The method for producing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons according to claim 6, wherein the preparation method of the stiff silkworm extract is as follows: grinding dried stiff silkworm into powder, adding 8 times of ethanol with the volume concentration of 75%, soaking for 10-15 minutes, heating in a water bath at 55-65 ℃ for 10-14 hours, reflux extracting for 2-3 times, heating and evaporating to remove ethanol, stirring at 300-800rpm for 20-60 minutes under the water bath condition of 40-60 ℃ for 1/10-1/20 of the volume concentration, and sterilizing to obtain the stiff silkworm extract.
10. The method for producing the traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons according to claim 6, wherein the preparation method of the mistletoe extract is as follows: pulverizing dried stem and leaf of herba Taxilli into coarse powder, and placing into supercritical CO 2 Adding 5 times volume of ethanol as entrainer into the extraction device, and extracting under 35-65MPa at 25-75deg.C to obtain herba Taxilli extract.
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202410187266.5A CN117731712B (en) | 2024-02-20 | 2024-02-20 | Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN202410187266.5A CN117731712B (en) | 2024-02-20 | 2024-02-20 | Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons |
Publications (2)
Publication Number | Publication Date |
---|---|
CN117731712A true CN117731712A (en) | 2024-03-22 |
CN117731712B CN117731712B (en) | 2024-05-17 |
Family
ID=90261285
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN202410187266.5A Active CN117731712B (en) | 2024-02-20 | 2024-02-20 | Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN117731712B (en) |
Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1229658A (en) * | 1998-03-24 | 1999-09-29 | 刘月江 | Compound sanbi medicinal liquor for treating gout |
CN1375298A (en) * | 2001-01-04 | 2002-10-23 | 黄兆元 | Pain-relieving notoginseng mixture |
US20060189512A1 (en) * | 2005-01-21 | 2006-08-24 | Ehrenkranz Joel R L | Compositions containing botanical extracts rich in phlorizin and methods for using such compositions in blood glucose modification and to affect aging |
US20090310839A1 (en) * | 2008-06-11 | 2009-12-17 | Katzenelson Omer Y | Systems and methods to perform inhibition diagnostic testing |
WO2013062266A2 (en) * | 2011-10-25 | 2013-05-02 | 주식회사 아모레퍼시픽 | Wrap fermentation method using a medicinal flower, and composition for external application onto the skin using same |
CN106924433A (en) * | 2015-12-31 | 2017-07-07 | 于清 | Promoting blood circulation medicinal liquor |
CN106929261A (en) * | 2015-12-31 | 2017-07-07 | 于清 | A kind of blood-activating wine |
CN106929306A (en) * | 2015-12-31 | 2017-07-07 | 于清 | One kind promoting blood circulation medicinal liquor |
CN107469006A (en) * | 2017-09-07 | 2017-12-15 | 成都兴倍加生物科技有限责任公司 | It is a kind of to be used to treat tincture dose medicament for the treatment of rheumatic ostealgia and preparation method thereof |
CN108815462A (en) * | 2018-07-06 | 2018-11-16 | 广州聚澜健康产业研究院有限公司 | A kind of pain relieving ointment and preparation method thereof |
CN109512994A (en) * | 2019-01-16 | 2019-03-26 | 罗朝艳 | A kind of Chinese medicine wine for external use that treating rheumatoid disease and preparation method |
CN114886958A (en) * | 2022-06-17 | 2022-08-12 | 唐山冀东老年病研究所 | Traditional Chinese medicine formula of electrostatic acupoint plaster for meridian acupoint diagnosis and therapy |
-
2024
- 2024-02-20 CN CN202410187266.5A patent/CN117731712B/en active Active
Patent Citations (12)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1229658A (en) * | 1998-03-24 | 1999-09-29 | 刘月江 | Compound sanbi medicinal liquor for treating gout |
CN1375298A (en) * | 2001-01-04 | 2002-10-23 | 黄兆元 | Pain-relieving notoginseng mixture |
US20060189512A1 (en) * | 2005-01-21 | 2006-08-24 | Ehrenkranz Joel R L | Compositions containing botanical extracts rich in phlorizin and methods for using such compositions in blood glucose modification and to affect aging |
US20090310839A1 (en) * | 2008-06-11 | 2009-12-17 | Katzenelson Omer Y | Systems and methods to perform inhibition diagnostic testing |
WO2013062266A2 (en) * | 2011-10-25 | 2013-05-02 | 주식회사 아모레퍼시픽 | Wrap fermentation method using a medicinal flower, and composition for external application onto the skin using same |
CN106924433A (en) * | 2015-12-31 | 2017-07-07 | 于清 | Promoting blood circulation medicinal liquor |
CN106929261A (en) * | 2015-12-31 | 2017-07-07 | 于清 | A kind of blood-activating wine |
CN106929306A (en) * | 2015-12-31 | 2017-07-07 | 于清 | One kind promoting blood circulation medicinal liquor |
CN107469006A (en) * | 2017-09-07 | 2017-12-15 | 成都兴倍加生物科技有限责任公司 | It is a kind of to be used to treat tincture dose medicament for the treatment of rheumatic ostealgia and preparation method thereof |
CN108815462A (en) * | 2018-07-06 | 2018-11-16 | 广州聚澜健康产业研究院有限公司 | A kind of pain relieving ointment and preparation method thereof |
CN109512994A (en) * | 2019-01-16 | 2019-03-26 | 罗朝艳 | A kind of Chinese medicine wine for external use that treating rheumatoid disease and preparation method |
CN114886958A (en) * | 2022-06-17 | 2022-08-12 | 唐山冀东老年病研究所 | Traditional Chinese medicine formula of electrostatic acupoint plaster for meridian acupoint diagnosis and therapy |
Non-Patent Citations (3)
Title |
---|
REDZIC SS ET AL.: "The ecological aspect of ethnobotany and ethnopharmacology of population in Bosnia and Herzegovina", 《COLLEGIUM ANTROPOLOGICUM》, vol. 31, no. 3, 30 September 2007 (2007-09-30), pages 869 - 890 * |
樊建;沈莹;赵娟萍;孙雪晴;关枫;韩凤娟;王艳宏;: "用于风湿病治疗的中药外用膏剂的应用现状及研究进展", 中国实验方剂学杂志, no. 02, 29 June 2018 (2018-06-29) * |
王鑫国: "《中药药理学实验教程》", 31 March 2017, 中国中医药出版社, pages: 13 * |
Also Published As
Publication number | Publication date |
---|---|
CN117731712B (en) | 2024-05-17 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN102091202B (en) | Traditional Chinese medicinal composition for treating fracture and preparation method thereof | |
CN103520654B (en) | A kind of Chinese medicine composition being used for the treatment of knee osteoarthritis and preparation method thereof | |
CN103656238B (en) | A kind of Chinese medicine composition for the treatment of rheumatic osteopathia | |
CN101244252A (en) | Chinese medicine label for eliminating ache and preparation thereof | |
CN107397949A (en) | A kind of ointment for treating neck-shoulder pain, lumbago and leg pain and preparation method thereof | |
CN104306495B (en) | A kind of Chinese medicine for external application of cure scapulohumeral periarthritis and preparation method thereof | |
CN104666698A (en) | External-use traditional Chinese medicine ointment for treating gonarthromeningitis and preparation method of traditional Chinese medicine ointment | |
CN104666579A (en) | Traditional Chinese medicine composition for treating gonarthromeningitis and preparation method of traditional Chinese medicine composition | |
CN104258278B (en) | Gushangling (for bone injury) spray | |
CN108175795A (en) | A kind of plaster | |
CN106975063A (en) | A kind of new bone setting plaster and preparation method thereof | |
CN100386106C (en) | Medicine for treating acne and its preparation | |
CN117731712B (en) | Production method of traditional Chinese medicine tincture for promoting blood circulation and relaxing tendons | |
CN101057922A (en) | Traditional Chinese medicine preparation for treating vitiligo | |
CN110141622B (en) | Traditional Chinese medicine composition for treating pain and preparation method and application thereof | |
CN108619464B (en) | Traditional Chinese medicine composition for treating rheumatoid arthritis and preparation method and application thereof | |
CN1049826C (en) | "Biantianfengshiqing" medicine for treating rheumatism | |
CN107213238B (en) | Traditional Chinese medicine for treating venomous snake bite and preparation method thereof | |
CN101721547B (en) | Medicine for treating onychomycosis | |
CN109908298A (en) | A kind of bone fracture bruise external preparation and preparation method thereof | |
CN112755134B (en) | Plaster for treating hyperosteogeny and preparation method thereof | |
CN110141621B (en) | Traditional Chinese medicine composition for treating pain and preparation method and application thereof | |
CN107158322A (en) | It is a kind of to be used for gout, soft tissue injury, preparation of rheumatalgia pain and preparation method thereof | |
CN105560427A (en) | Transdermal gate-freeing powder | |
CN105560537A (en) | Medicine composition for treating osteoartritis and preparation method thereof |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
GR01 | Patent grant | ||
GR01 | Patent grant |