CN117582834A - Inspection pretreatment multichannel liquid preparation system and liquid preparation method - Google Patents
Inspection pretreatment multichannel liquid preparation system and liquid preparation method Download PDFInfo
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- CN117582834A CN117582834A CN202311492427.3A CN202311492427A CN117582834A CN 117582834 A CN117582834 A CN 117582834A CN 202311492427 A CN202311492427 A CN 202311492427A CN 117582834 A CN117582834 A CN 117582834A
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- 239000007788 liquid Substances 0.000 title claims abstract description 244
- 238000007689 inspection Methods 0.000 title claims abstract description 15
- 238000002360 preparation method Methods 0.000 title abstract description 26
- 239000012452 mother liquor Substances 0.000 claims abstract description 308
- 239000003085 diluting agent Substances 0.000 claims abstract description 179
- 238000012360 testing method Methods 0.000 claims abstract description 66
- 239000000243 solution Substances 0.000 claims abstract description 29
- 238000007865 diluting Methods 0.000 claims abstract description 23
- 238000004140 cleaning Methods 0.000 claims abstract description 18
- 239000012086 standard solution Substances 0.000 claims abstract description 17
- 238000010790 dilution Methods 0.000 claims description 162
- 239000012895 dilution Substances 0.000 claims description 162
- 238000003860 storage Methods 0.000 claims description 57
- 238000009826 distribution Methods 0.000 claims description 54
- 238000011084 recovery Methods 0.000 claims description 49
- 230000002572 peristaltic effect Effects 0.000 claims description 47
- 238000010926 purge Methods 0.000 claims description 42
- 239000010413 mother solution Substances 0.000 claims description 27
- 239000002699 waste material Substances 0.000 claims description 24
- 238000000926 separation method Methods 0.000 claims description 21
- 238000000034 method Methods 0.000 claims description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 230000009471 action Effects 0.000 claims description 7
- 238000005259 measurement Methods 0.000 claims description 7
- 238000007599 discharging Methods 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000006243 chemical reaction Methods 0.000 claims description 2
- 230000000149 penetrating effect Effects 0.000 claims description 2
- 238000013461 design Methods 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 3
- 238000012423 maintenance Methods 0.000 abstract 1
- 238000004891 communication Methods 0.000 description 13
- 239000012085 test solution Substances 0.000 description 11
- 230000009977 dual effect Effects 0.000 description 10
- 230000008569 process Effects 0.000 description 10
- 238000010586 diagram Methods 0.000 description 7
- 239000012530 fluid Substances 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 238000001514 detection method Methods 0.000 description 5
- 230000036541 health Effects 0.000 description 5
- 210000000080 chela (arthropods) Anatomy 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 239000002537 cosmetic Substances 0.000 description 3
- 238000012864 cross contamination Methods 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 230000007613 environmental effect Effects 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 238000002347 injection Methods 0.000 description 3
- 239000007924 injection Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 230000006837 decompression Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000003754 machining Methods 0.000 description 2
- 238000004064 recycling Methods 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000013022 venting Methods 0.000 description 2
- 238000009736 wetting Methods 0.000 description 2
- 238000010276 construction Methods 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 239000013013 elastic material Substances 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
Classifications
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/45—Mixing liquids with liquids; Emulsifying using flow mixing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/405—Methods of mixing liquids with liquids
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F23/00—Mixing according to the phases to be mixed, e.g. dispersing or emulsifying
- B01F23/40—Mixing liquids with liquids; Emulsifying
- B01F23/49—Mixing systems, i.e. flow charts or diagrams
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01F—MIXING, e.g. DISSOLVING, EMULSIFYING OR DISPERSING
- B01F2101/00—Mixing characterised by the nature of the mixed materials or by the application field
- B01F2101/23—Mixing of laboratory samples e.g. in preparation of analysing or testing properties of materials
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- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a test pretreatment multichannel liquid preparation system and a liquid preparation method. The diluent supply module can be provided with a diluent switching accessory I or II, and the mother liquor supply module can be provided with a mother liquor switching accessory I or II. The system can realize multiple functions of preparing standard curve solution, preparing mixed standard solution, precisely taking liquid, precisely diluting, automatically cleaning, rinsing, recovering solution and the like in one step. The system adopts a modularized design, has compact structure, high treatment efficiency, convenient module disassembly and assembly and convenient maintenance, can flexibly configure the module according to the inspection task, solves the defects of manually preparing the solution to be tested in the past, and can greatly improve the solution preparation efficiency in the inspection pretreatment work.
Description
Technical Field
The invention relates to a test pretreatment multichannel liquid preparation system and a liquid preparation method, which are used in the field of sample pretreatment of all test detection industries (including but not limited to foods, medicines, health products, cosmetics, agricultural products and environments).
Background
In the analysis and detection of foods, medicines, health products, cosmetics, agricultural products, environmental samples and the like, the preparation of a test solution is an indispensable link. The analysis and detection of the sample mainly comprises four steps, and the sequence is as follows: pretreatment of a sample, filtration of a pretreatment solution, distribution and dilution of the solution, and detection of the solution. The device of the invention is used for the third step. At present, in the preparation work of a test solution, manual operation is relied on under most conditions, and the defects that the solution preparation efficiency is low, the time limit requirements of burst test and high-throughput test are difficult to deal with, the experimental environment is not attractive, the operation is complex, the capacity instrument is placed in disorder, the labels are disordered, the organic solvent is volatile and harmful to health, the measuring precision is insufficient, the labor intensity is high, misoperation is easy to occur and the like exist. There are also some automatic or semi-automatic test solution preparation aids on the market, such as: the liquid-collecting device comprises a multichannel dilution liquid-collecting device adopting a peristaltic pump, a standard-mixing device adopting a syringe pump and used for preparing mixed element standard solutions, a liquid-collecting device adopting a negative pressure principle and singly used for collecting liquid with common precision, a liquid-storing ring-type liquid-collecting device adopting the negative pressure principle and a non-direct contact liquid-storing ring with redundant volume and singly used for collecting various solutions with common precision, and the like. Although the instruments and equipment realize automation or semi-automation, the instruments and equipment still have the defects of insufficient measuring precision, single function, lack of modular design concept, high instrument price, low running speed, large solution waste volume and the like. Therefore, developing a new generation of full-automatic test solution preparation system and preparation method has become an urgent need in the inspection and detection industry.
Disclosure of Invention
The invention provides a test pretreatment multichannel liquid preparation system and a liquid preparation method, which can effectively solve the problems of low preparation efficiency of test solutions, solvent waste, messy placement, disordered labels, easy misoperation, poor precision and other common problems of test industries in the process of analyzing and detecting samples such as foods, medicines, health products, cosmetics, agricultural products, environmental samples and the like.
In order to solve the technical problems, the invention adopts the following technical scheme:
an inspection pretreatment multichannel liquid distribution system, comprising:
a diluent supply module for supplying a diluent;
the diluent switching accessory I comprises a first multichannel valve; the diluent switching accessory I is connected with the diluent supply module and is used for uniformly distributing the supplied diluent to all channels of the first multi-channel valve; so that 8 channels can obtain the dilution liquid simultaneously and uniformly.
The diluent switching accessory II comprises a second multichannel valve; the diluent switching accessory I is connected with the diluent supply module and is used for uniformly distributing the supplied diluent to all channels of the second multi-channel valve; the 8 channels can simultaneously and uniformly obtain the diluent, and the diluent switching accessory is different from the diluent switching accessory in that a single eccentric rotary manual/automatic dual-purpose valve is added, so that the diluent is additionally obtained into a single eccentric valve recycling pipe channel for realizing independent precise measurement and recycling of the diluent.
A mother liquor supply module for supplying mother liquor;
the mother liquor switching accessory I comprises a third multichannel valve; for supplying 8 kinds of mother liquor to each high-frequency seven-way solenoid valve individually.
The mother liquor switching accessory II comprises a fourth multichannel valve; for providing 8 kinds of mother liquor separately to each high-frequency seven-way solenoid valve; the mother liquor switching accessory is different from the mother liquor switching accessory in that 8 single eccentric rotary manual-automatic dual-purpose valves are added, so that each path of mother liquor additionally obtains a multi-eccentric valve recovery pipe channel for realizing independent precise measurement and recovery of the mother liquor.
And one end of the flow path distribution module is connected with the first diluent switching accessory or the second diluent switching accessory, and the other end of the flow path distribution module is connected with the first mother liquor switching accessory or the second mother liquor switching accessory.
Compared with the prior art, the invention has the beneficial effects that:
1. the invention adopts modularized design, has high degree of automation, can realize the functions of preparing standard curve solution in one step, preparing mixed standard solution in one step, preparing internal standard mother solution in one step, precisely taking liquid, precisely diluting, automatically cleaning and rinsing, recovering mother solution and diluent, and the like, has good universality and rich functions, can obviously improve the preparation efficiency of test solution, reduces the waste of standard substances and diluent solvents, and is beneficial to environmental protection.
2. The invention creates five fluid switching valves, which are respectively: t-shaped manual-automatic dual-purpose valve, single eccentric rotation manual-automatic dual-purpose valve, multi-eccentric rotation manual-automatic dual-purpose valve, cylindrical multi-way manual-automatic dual-purpose valve and high-frequency seven-way electromagnetic valve. The T-shaped manual/automatic dual-purpose valve can uniformly decompose one path of diluent into eight paths, and can be magnetically controlled and manually controlled, so that the reliability of the system is improved; the single eccentric rotation manual-automatic dual-purpose valve and the multi-eccentric rotation manual-automatic dual-purpose valve can realize the extraction, supply, preparation and recovery of mother solution and diluent, can be controlled electrically and manually, and improve the reliability of the system; the cylindrical multi-way manual-automatic dual-purpose valve can realize extraction, supply and preparation of mother liquor, can realize pneumatic control and manual control, and improves the reliability of the system; the high-frequency seven-way electromagnetic valve can be provided with a liquid storage ring or a quantitative ring, wherein the quantitative ring mode is faster in preparation speed, millisecond-level flow/stop of mother liquid and diluent can be realized, and the preparation precision of the test liquid and the durability of equipment are improved. The five fluid switching valves can effectively avoid cross contamination of different flow paths, and cooperatively operate under the liquid distribution method of the embodiment, so that the accuracy of analysis results is improved.
3. The invention is provided with the bubble detector, so that the preparation precision of the test solution can be effectively judged; the system has high tightness and corrosion resistance, and can effectively protect the health of the inspector.
4. The invention comprises a test pretreatment multichannel liquid distribution system and a liquid distribution method, which are easy to realize programming control.
5. The invention has the advantages of convenient disassembly and assembly of all accessories, and provides different system configurations according to different inspection tasks, thereby reducing the use cost of users.
Drawings
FIG. 1 is a general composition of the present invention;
FIG. 2 is a block diagram of a diluent supply module of the present invention;
FIG. 3 is a block diagram of a diluent switching accessory one of the present invention;
FIG. 4 is a block diagram of a diluent switching accessory II of the present invention;
FIG. 5 is a front view of the mother liquor supply module of the present invention;
FIG. 6 is a side view of the mother liquor supply module of the present invention;
FIG. 7 is a block diagram of a mother liquor switch accessory one of the present invention;
FIG. 8 is a block diagram of a mother liquor switch accessory II of the present invention;
FIG. 9 is a side view of a single high frequency seven-way solenoid valve of the flow path distribution module of the present invention;
FIG. 10 is a top view of a single high frequency seven-way solenoid valve of the flow path distribution module of the present invention;
FIG. 11 is a block diagram of a purge module of the present invention;
FIG. 12 is a perspective view of a T-shaped manual and automatic dual purpose valve of the present invention;
FIG. 13 is a perspective view of a single eccentric rotary manual dual purpose valve of the present invention;
FIG. 14 is a perspective view of a cylindrical multi-way manual and automatic dual purpose valve of the present invention;
FIG. 15 is a perspective view of a high frequency seven-way solenoid valve of the present invention;
FIG. 16 is a perspective view of the check head of the present invention;
FIG. 17 is a schematic diagram of a high frequency seven-way solenoid valve switch of the present invention (seven-way valve positions one-eight in sequence from top to bottom on the left, seven-way valve open position on the right);
fig. 18 is a perspective view of a second mother liquor switch attachment of the present invention.
Wherein: 1. a diluent supply module; 2. diluent switching accessory one; 3. diluent switching accessory II; 4. a mother liquor supply module; 5. mother liquor switching accessories I; 6. mother liquor switching accessories II; 7. a flow path distribution module; 8. a purge module; 101. dilution supply module post 102. Dilution supply module lead screw; 103. a dilution supply module upper platform; 104. a dilution supply module upper platform jack; 105. a dilution supply module receiving hole; 106. a dilution supply module lower platform; 107. a dilution supply module lower platform jack; 108. dilution feed module motor; 109. diluting and supplying a module motor driving gear; 110. diluting and supplying a module motor driven gear; 111. a belt; 112. a dilution supply module drive rack; 113. a dilution supply module L bracket fixing hole; 114. diluting the piston plunger; 115. diluting a piston inserted rod fixing wire; 116. diluting the piston double-clip; 117. a dilution supply module L bracket; 118. a diluting supply module L bracket connecting hole; 119. a dilution piston; 120. perforating a dilution piston; 121. diluting the piston conical head; 122. diluting the conical head tip of the piston; 123. diluting a piston sealing ring; 124. diluting a sample injector; 125. diluting the injector head; 126. diluting the outer thread of the sample injector head; 127. diluting the sample injection hole; 128. the dilution supply module drives the wire hole of the frame; 129. a screw; t-shaped manual/automatic dual-purpose valve; a t-shaped lumen; t-shaped manual/automatic dual-purpose valve manual pulling window; t-shaped manual/automatic dual-purpose valve fixing hole; t-valve inlet; t-valve outlet; t valve diluent inlet; 208. the flat end of the diluent inlet pipe; 209T valve diluent inlet joint; 210. the diluent enters the outer thread of the pipe joint; 211. a diluent outlet pipe; 212. the diluted liquid is discharged from the flat end; 213. a diluent outlet pipe joint; 214. a diluent outlet pipe joint external thread; 215. a single bubble sensor; manual scales of T-shaped manual and automatic two-purpose valves; t-spool handle; t-spool; t-spool magnetic pad; a T-shaped valve core enters a sample injector channel; a T-shaped valve core is arranged outside a sample injector channel; t-valve dilution interface; t-valve dilution interface passage; 224. an inlet channel of the dilution liquid separation cavity; 225. a center channel of the dilution liquid separation cavity; 226. a diluting liquid separation cavity L channel; 227. a diluent inlet passage; 301. single eccentric rotary manual and automatic dual-purpose valve; 302. a single offset valve fixing hole; 303. a single-bias valve core distribution main way; 304. a single bias valve recovery port; 305. a single bias valve inlet; 306. a single bias valve motor fixing plate; 307. a single bias valve motor; 308. a single bias valve handle; 309. screw holes of a motor fixing plate of the single offset valve; 310. a single bias valve outlet; 311. a single offset valve recovery pipe; 312. a single-offset valve inlet pipe; 313. single bias valve motor fixing plate hole; 314. a single bias spool outlet channel; 315. a single bias spool recovery channel; 316. a single-bias spool inlet channel; 317. a single bias spool; 318. a single-offset valve motor fixing hole; 319. a single bias valve dilution interface; 320. a single offset valve dilution interface passage; 321 single offset valve lumen; 322. a single-bias valve liquid separation cavity central channel; 323. a single-bias valve liquid separation cavity L channel; 401. a mother solution is supplied to the upper platform of the module; 402. a mother liquor supply module support column; 403. a mother liquor supply module lower platform; 404. mother liquor co-driving frame; 405. a mother liquor supply module L bracket; 406. a mother solution supply module receiving hole; 407. the mother solution is supplied to the module motor; 408. a mother solution is supplied to a driving gear of a motor of the module; 409. mother solution is supplied to the double driven gears of the module motor; 410. mother liquor supply module double screw rods; 411. a mother liquor injector; 412. a mother liquor injector head; 413. a mother solution injector head external thread; 414. a mother solution sample injection hole; 415. a mother liquor piston; 416. mother liquor piston inserting rod; 417. a mother liquor inlet pipe; 418. the mother liquor enters the flat end of the pipe; 419. a mother liquor inlet pipe joint; 420. mother liquor enters the outer thread of the pipe joint; 421. a multi-linked bubble sensor; 422. a mother liquor outlet pipe; 423. a mother liquor outlet pipe joint; 424. mother liquor co-drives the rack slide hole; 425. a mother liquor co-driving frame driving hole; 426. mother liquor co-drives the hollow groove of the frame; 427. mother liquor co-drives the rack connecting hole; 428. mother liquor piston inserted rod fixing wire; 429. the thick end of the mother liquor piston; 430. a mother liquor piston conical head; 431. conical head tip of mother liquor piston; 432. a mother liquor piston seal ring; 433. mother liquor piston double-clamping; 434. mother liquor supply module L bracket fixing hole; 435. perforating a mother liquor piston; 501. cylindrical multi-way manual-automatic dual-purpose valve; 502. a cylindrical lumen; 503. a cylindrical multiway valve fixing hole; 504. a cylindrical multiport valve inlet; 505. a cylindrical multi-way valve outlet; 506. a cylindrical multi-way valve pneumatic tube I; 507. cylindrical multi-way valve pneumatic tube II; 508. a gas pipe I gas leakage port; 509. a second gas leakage port of the pneumatic pipe; 510. a cylindrical multi-way valve core hand-dialing window; 511. a cylindrical multi-way valve stem handle; 512. a cylindrical multi-way valve core; 513. a cylindrical multi-way valve cartridge inlet passage; 514. a cylindrical multi-way valve cartridge outlet passage; 515. cylindrical multi-way valve pneumatic piston; 516. a mother liquor interface of the cylindrical multi-way valve; 517. a mother liquor interface channel of a cylindrical multi-way valve; 518. a cylindrical multi-way valve inlet passage; 519. a cylindrical multi-way valve outlet passage; 520. a cylindrical multi-way valve pneumatic valve; 521. a cylindrical multi-way valve pneumatic chamber; 601. multiple eccentric rotary manual and automatic dual-purpose valve; 602. a multi-bias valve fixing hole; 603. a multi-bias valve motor; 604. a multi-bias valve handle; 605. a multi-bias valve motor fixing plate; 606. a multi-bias valve motor fixing hole; 607. a multi-bias valve outlet; 608. a multi-bias valve recovery port; 609. a multi-bias valve inlet; 610. a multi-bias valve outlet pipe; 611. a multi-bias valve recovery pipe; 612. a multi-bias valve inlet pipe; 701. a high-frequency seven-way electromagnetic valve; 702. seven-way valve upper cavity; 703. a seven-way valve lower cavity; 704. a seven-way valve upper motor; 705. a seven-way valve upper piston; 706. a motor fixing plate on the seven-way valve; 707. a motor fixing hole is arranged on the seven-way valve; 708. seven-way valve lower motor; 709. a seven-way valve lower piston; 710. seven-way valve lower motor fixing plate; 711. seven-way valve lower cavity interface; 712. a reservoir or dosing ring; 713. a test liquid tube; 714. a seven-way valve joint; 715. a reservoir or dosing ring inlet; 716. a reservoir or dosing ring outlet; 717. seven-way valve waste liquid interface; 718. seven-way valve mother liquor interface; 719. a seven-way valve diluent port; 720. a seven-way valve purge port; 721. a reservoir or dosing ring inlet port; 722. a reservoir ring or dosing ring outlet port; 723. a seven-way valve test liquid interface; 724. a test tube; 725. seven-way valve core magnetic pad; 726. seven-way valve core fixing edges; 727. a seven-way valve core; 728. a motor fixing hole is arranged on the seven-way valve; 729. seven-way valve lower motor fixing hole; 730. an inner cavity of the seven-way valve; 731. seven-way valve cavity groove; 732. a seven-way valve switch passage; 733. a piston coil is arranged on the seven-way valve; 734. seven-way valve lower piston coil; 735. a seven-way valve core body; 736. a return stop; 737. a check head internal passage; 738. a check head inlet; 739. a check head outlet; 801. a peristaltic pump; 802. peristaltic tube limiting clamp; 803. an extruder; 804. an extrusion column; 805. a limit screw adjusting groove; 806. a limit screw; 807. a peristaltic pump motor; 808. a peristaltic tube;
Detailed Description
The invention is described in detail below with reference to the attached drawings:
the invention provides an inspection pretreatment multichannel liquid distribution system and an inspection pretreatment multichannel liquid distribution method.
1. Liquid preparation system:
comprises a diluent supply module 1, a mother liquor supply module 4, a flow path distribution module 7 and a purging module 8. Wherein, the diluent supply module 1 can be provided with a diluent switching accessory I2 or II 3, and the mother liquor supply module 4 can be provided with a mother liquor switching accessory I5 or II 6. The first diluent switching accessory 2 comprises a T-shaped manual/automatic dual-purpose valve 201 and a connecting pipeline, the second diluent switching accessory 3 comprises a single eccentric rotation manual/automatic dual-purpose valve 301 and a connecting pipeline, the first mother liquid switching accessory 5 comprises a cylindrical multi-way manual/automatic dual-purpose valve 501 and a connecting pipeline, and the second mother liquid switching accessory 6 comprises a multi-eccentric rotation manual/automatic dual-purpose valve 601 and a connecting pipeline.
The T-shaped manual/automatic dual-purpose valve 201 can enable diluent to be rapidly switched between input and output through two channels (one channel can be uniformly distributed into 8 channels) which are mutually independent, does not have cross contamination, enables the diluent to be uniformly divided into 8 channels during output, provides the diluent for 8 high-frequency seven-way electromagnetic valves 701, realizes manual and magnetic control dual-purpose, can be broken, and improves reliability. The single eccentric rotation manual-automatic dual-purpose valve 301 has the functions of the T-shaped manual-automatic dual-purpose valve 201, and can supplement and enable diluent to be quickly and freely switched between input, output and recovery without crossing at the front end of the high-frequency seven-way electromagnetic valve 701, so that redundant diluent can be timely returned to a reagent bottle, diluent waste is reduced, manual control and electric control dual-purpose are realized, and quick and precise diluent measurement can be realized.
The cylindrical multi-way manual-automatic dual-purpose valve 501 can enable mother liquor to be rapidly switched between input and output through 16 channels which are mutually independent, does not have cross contamination, can respectively provide 8 mother liquor for 8 high-frequency seven-way electromagnetic valves 701, realizes dual purposes of manual control and gas automatic control, can also break a circuit, and can improve the reliability of the movement of the cylindrical multi-way valve core 512 inside the cylindrical multi-way manual-automatic dual-purpose valve 501. The multi-eccentric rotary manual-automatic dual-purpose valve 601 has the functions of the cylindrical multi-way manual-automatic dual-purpose valve 501, and can supplement 8 kinds of mother solutions which are respectively independent and can be quickly and freely switched between input, output and recovery at the front end of the high-frequency seven-way electromagnetic valve 701, so that redundant mother solutions can be timely returned to a reagent bottle, waste of the mother solutions is reduced, manual control and electric control dual-purpose are realized, and quick and precise mother solution measurement can be realized.
The high-frequency seven-way electromagnetic valve 701 is provided with seven interfaces, which are respectively connected with an inlet and an outlet of waste liquid, mother liquid, diluent, a purging pipe, a liquid storage ring or a quantitative ring (alternative one), and a test liquid, wherein 8 layers in the horizontal direction are arranged inside the seven-way valve core 735, each layer is provided with a processed arc-shaped inner channel, and the channels are mutually independent and do not cross, and can vertically move in the inner cavity 730 of the seven-way valve at a general speed or ultra-fast (millisecond level) under the actions of the pushing of an upper piston and a lower piston and a magnetic coil inside the piston, so as to realize 8 preset connection and disconnection. The 8 preset communication can realize 9 functions of cleaning a system pipeline, cleaning a liquid storage ring or a quantitative ring, drying the liquid storage ring or the quantitative ring, wetting mother liquor, precisely taking the mother liquor, wetting the diluent, precisely taking the diluent, discharging the test solution and cutting off all passages.
The purging module 8 comprises a peristaltic pump 801 and 8 peristaltic tubes 808, wherein the peristaltic tubes are made of elastic materials, and the peristaltic tubes are driven to perform pulse motion through a peristaltic pump motor 807, so that 8 paths of air respectively enter 8 high-frequency seven-way electromagnetic valves 701 to play a role in evacuating liquid in a rear-end pipeline.
In this embodiment, through the program control of the T-type manual-automatic dual-purpose valve 201, the single eccentric rotary manual-automatic dual-purpose valve 301, the cylindrical multi-way manual-automatic dual-purpose valve 501, the multi-eccentric rotary manual-automatic dual-purpose valve 601, the high-frequency seven-way electromagnetic valve 701, the purging module 8, the diluent and mother solution injector, various motors, the pneumatic valves, and the flexible combination among the modules, the standard curve solution (the concentration of a single unit is at most 8, the number of the single unit can be arbitrarily increased), the mixed standard solution (the standard solution of a single unit is at most 8, the number of the single unit can be arbitrarily increased), the functions of preparing the mother solution containing an internal standard in a single unit, precisely taking the liquid, precisely diluting, automatically cleaning and rinsing, recovering the redundant mother solution and the diluent and the like can be realized, and the efficiency of preparing the test solution can be greatly improved.
(1) Diluent supply module 1 (fig. 2):
comprises a vertical fixing frame, a dilution supply module support 101, a dilution supply module upper platform 103 and a dilution supply module lower platform 106; the upper platform jack 104 of the dilution supply module and the lower platform jack 107 of the dilution supply module are used for inserting the screw rod 102 of the dilution supply module, and the lower part of the screw rod passes through the lower platform jack to be connected with the driven gear 110 of the motor of the dilution supply module. The dilute feed module motor 108 rotates the feed module motor driven gear 110 through the dilute feed module motor driving gear 109 and the belt 111. The dilution feed module drive bay 112 has a double dilution feed module drive bay wire aperture 128 that moves vertically up and down as the screw rotates. The dilution supply module L bracket 117 is fixed to the dilution supply module driving frame 112 by screws in the dilution supply module L bracket fixing holes 113, and a dilution supply module L bracket connecting hole 118 is provided at the bottom of the dilution supply module L bracket 117, and the dilution piston insert rod fixing wire 115 can be screwed correspondingly. Dilution piston plunger 114 may be inserted through dilution piston bore 120 to secure cylindrical dilution piston 119 by two semi-circular dilution piston double clips 116, allowing dilution supply module motor 108 to drive dilution piston 119 up and down. The top of dilution piston 119 is connected to dilution piston conical head 121, which mates with the inside top of dilution syringe 124. The dilution piston sealing ring 123 is a double-layer elastic rubber ring and plays a role in sealing. The top of the dilution piston cone 121 has a dilution piston cone tip 122 that acts to reduce dead volume.
The dilution supply module receiving holes 105 are four symmetrical on the dilution supply module upper platform 103 for inserting screws 129 (fig. 4) to fix the T-type manual/automatic dual purpose valve 201 or the single eccentric rotation manual/automatic dual purpose valve 301. Dilution injector head 125 may be inserted into either T-valve dilution interface 222 (fig. 12) or single offset valve dilution interface 319 (fig. 13) and secured and sealed by dilution injector head outer wire 126 such that dilution injection orifice 127 is tightly connected to either T-valve dilution interface channel 223 (fig. 12) or single offset valve dilution interface channel 320 (fig. 13).
(2) Diluent switch accessory one 2 (fig. 3 and 12):
the valve comprises a T-shaped manual/automatic dual-purpose valve 201 and a connecting pipeline, wherein a left-right and front-penetrating T-shaped inner cavity 202 (figure 12) is formed in the T-shaped manual/automatic dual-purpose valve 201, a T-shaped valve core 218 is tightly embedded in the T-shaped inner cavity, a T-shaped valve core magnetic pad 219 is respectively arranged at the left end and the right end of the T-shaped valve core 218, and the T-shaped valve core 218 can move left and right under the action of magnetic force. The T-shaped valve core handle 217 can move left and right in the T-shaped manual/automatic dual-purpose valve hand pulling window 203, and can also drive the T-shaped valve core 218 to move left and right. The T-shaped spool 218 has a T-spool inlet injector passage 220 and a T-spool outlet injector passage 221 therein. The T-valve inlet injector passage 220 communicates with a T-valve dilution interface passage 223 and a dilution liquid distribution chamber inlet passage 224. The T-spool outlet injector channel 221 communicates with a T-valve dilution interface channel 223 and a diluent inlet channel 227.
A T-valve diluent inlet fitting 209 (fig. 3) is screwed into the T-valve inlet 205 (fig. 12) through a diluent inlet fitting external thread 210, in close connection with the diluent inlet channel 227 below. The flat end 208 of the diluent inlet tube acts to enhance sealing and prevent the T-valve diluent inlet tube fitting 209 from falling out.
The center channel 225 (fig. 12) of the dilution and separation cavity is communicated above the inlet channel 224 of the dilution and separation cavity, and is a flat cylinder chamber, and the center channel is communicated with the L channels 226 of 8 dilution and separation cavities, wherein the inner diameters are consistent, the angles are average, and the uniform diversion is ensured. The 8 diluent outlet pipe joints 213 are screwed into the T-shaped valve outlet 206 through the diluent outlet pipe joint external threads 214, and are respectively and closely communicated with the 8 diluent separating cavity L channels 226.
The T-shaped manual/automatic dual-purpose valve fixing hole 204 can be inserted with a screw to fix the T-shaped manual/automatic dual-purpose valve 201 on the upper platform 103 of the dilution supply module. Diluent enters from T-valve diluent inlet tube 207 and is drawn into dilution injector 124 when T-valve diluent interface channel 223 and diluent inlet channel 227 are in communication; is pushed out of dilution injector 124 when T-valve dilution interface passage 223 and dilution split chamber inlet passage 224 are in communication, and on average into seven-way valve dilution interface 719 (fig. 10) of 8 high-frequency seven-way solenoid valves 701. The outer wall of the T-valve diluent inlet tube 207 is provided with a single bubble sensor 215 for determining the accuracy of the volume of diluent flowing therethrough.
(3) Diluent switch accessory two 3 (fig. 4 and 13):
comprises a single eccentric rotary manual dual-purpose valve 301 and a connecting pipeline, wherein a cylindrical single-bias valve inner cavity 321 (figure 13) is formed in the top of the single-eccentric rotary manual dual-purpose valve 301 inwards (not penetrating) and is used for tightly embedding a single-bias valve core 317. The single-bias valve core 317 is internally provided with three independent L-shaped channels, and the three independent L-shaped channels can be circularly communicated with a single-bias valve dilution interface channel 320 at the eccentric position of the bottom of the single-bias valve core 317 under the drive of a single-bias valve motor 307 and are sequentially and circularly communicated with a single-bias valve core distribution main channel 303 or a single-bias valve recovery port 304 or a single-bias valve inlet 305.
Dilution injector head 125 may be screwed into single offset dilution interface 319 (fig. 13) via dilution injector head external thread 126 and tightly coupled to single offset dilution interface channel 320. When the single-bias valve motor 307 controls the single-bias valve dilution interface channel 320 to be communicated with the single-bias valve core distribution main channel 303, the single-bias valve motor is used for evenly conveying the diluent to the 8 single-bias valve liquid distribution cavity L channels 323 and then enters the seven-pass valve diluent interface 719 of the high-frequency seven-pass electromagnetic valve 701; when the single-offset valve motor 307 controls the single-offset valve dilution interface channel 320 to be communicated with the single-offset valve recovery port 304, the single-offset valve motor is used for recovering or precisely measuring the diluent through the single-offset valve recovery pipe 311; when the single-bias valve motor 307 controls the single-bias valve dilution interface passage 320 to communicate with the single-bias valve inlet 305, it is used to draw dilution liquid into the dilution sampler 124 through the single-bias valve inlet 312. The single bubble sensor 215 is also mounted on the outer wall of the single offset valve inlet tube 312 for determining the accuracy of the volume of diluent flowing therethrough.
(4) Mother liquor supply module 4 (fig. 5 and 6):
comprises a vertical fixing frame, which is composed of a mother liquor supply module upper platform 401, a mother liquor supply module lower platform 403 and two mother liquor supply module struts 402. Between the two mother liquor supply module supports 402, the mother liquor supply module upper platform 401 and the mother liquor supply module lower platform 403 each have two openings for passing through the mother liquor supply module double screw rod 410, below which a pair of mother liquor supply module motor double driven gears 409 are fixed, and one mother liquor supply module motor driving gear 408 is sandwiched between the two mother liquor supply module motor double driven gears 409. The mother liquor supply module motor 407 drives the mother liquor supply module motor driving gear 408 to rotate, and then drives the mother liquor supply module double lead screw 410 to rotate in the same direction at the same time.
The mother liquor co-drive frame 404 is of a partial double-layer structure, so that firmness is improved. A pair of mother liquor co-drive rack slide holes 424 are provided at each end of the double structure for sliding the mother liquor co-drive rack 404 over the two mother liquor feed module posts 402. Meanwhile, a pair of driving holes 425 of the mother liquor co-driving frame for processing the inner thread are arranged in the middle of the mother liquor co-driving frame 404 and are just tightly matched with the two mother liquor supply module double screw rods 410, so that when the two mother liquor supply module double screw rods 410 rotate in the same direction at the same time, the mother liquor co-driving frame 404 can be driven to slide on the two mother liquor supply module support columns 402.
The 8 mother liquor supply module L brackets 405 are fixed to the mother liquor common drive frame 404 as a unit by using screws through the mother liquor common drive frame receiving holes 427 and the mother liquor supply module L bracket fixing holes 434, thereby allowing the 8 mother liquor plungers 415 to move up and down at the same time. The bottom of the mother liquor piston 415 has a thickened mother liquor piston butt 429 for machining the mother liquor piston perforations 435.
The construction of the mother liquor piston plunger 416, mother liquor piston plunger retaining wire 428, mother liquor piston double clamp 433, mother liquor piston conical head 430, mother liquor piston conical head tip 431, mother liquor piston seal 432 is similar to that in the diluent supply module 1.
The outer walls of the 8 mother liquor inlet pipes 417 and the 8 mother liquor outlet pipes 422 are respectively penetrated through a multi-connected bubble sensor 421 for detecting whether bubbles exist in the liquid flowing through the multi-connected bubble sensor, so as to judge the volume accuracy of the mother liquor.
(5) Mother liquor switch attachment one 5 (fig. 7 and 14):
comprising a cylindrical multi-way manual/automatic dual-purpose valve 501 and connecting pipes. The cylindrical multi-way manual dual-purpose valve 501 is internally provided with a left, right and front through cylindrical inner cavity 502, and is tightly embedded into a cylindrical multi-way valve core 512 which has three positions of left, middle and right. A cylindrical multi-way valve stem handle 511 extends from the cylindrical multi-way valve stem hand-pulling window 510 at the front of the cylindrical multi-way valve stem 512 for manually adjusting the horizontal position of the cylindrical multi-way valve stem 512 within the cylindrical interior cavity 502.
Inside the cylindrical multi-way valve core 512, there are 8 cylindrical multi-way valve core inlet passages 513 and 8 cylindrical multi-way valve core outlet passages 514 which are independent of each other, the cylindrical multi-way valve core inlet passages 513 are communicated with the cylindrical multi-way valve mother liquid interface passages 517 and the cylindrical multi-way valve inlet passages 518, and the cylindrical multi-way valve core outlet passages 514 are communicated with the cylindrical multi-way valve mother liquid interface passages 517 and the cylindrical multi-way valve outlet passages 519. When the cylindrical multi-way valve cartridge handle 511 is to the left of the cylindrical multi-way valve cartridge dial window 510, the cylindrical multi-way valve mother liquor interface channel 517 and the cylindrical multi-way valve outlet channel 519 are in communication, at which point mother liquor in the lower mother liquor injector 411 may be pushed into the mother liquor outlet pipe 422. When the cylindrical multi-way valve stem handle 511 is in the middle of the cylindrical multi-way valve stem dial window 510, it is in the open circuit state. When the cylindrical multi-way valve cartridge handle 511 is to the right of the cylindrical multi-way valve cartridge dial window 510, the cylindrical multi-way valve mother liquor interface channel 517 and the cylindrical multi-way valve inlet channel 518 are in communication, at which point the lower mother liquor injector 411 may draw mother liquor from the mother liquor inlet tube 417.
The cylindrical multiport valve spool 512 has a cylindrical multiport valve pneumatic piston 515 on top of it, integral therewith. The cylindrical multi-way valve pneumatic piston 515 is precisely embedded into the cylindrical multi-way valve pneumatic valve 520, and can horizontally move between the left cylindrical multi-way valve pneumatic chamber 521 and the right cylindrical multi-way valve pneumatic chamber 521, so as to drive the cylindrical multi-way valve core 512 to horizontally move.
When compressed gas is flushed from the first cylindrical multi-way valve pneumatic tube 506, the cylindrical multi-way valve pneumatic piston 515 is pushed to move to the left edge of the right cylindrical multi-way valve pneumatic chamber 521, and the rear gas is discharged from the second pneumatic tube venting port 509 for decompression recovery, and the lower cylindrical multi-way valve mother liquor interface channel 517 is communicated with the cylindrical multi-way valve inlet channel 518. When compressed gas is flushed from the second cylindrical multi-way valve pneumatic tube 507, the cylindrical multi-way valve pneumatic piston 515 is pushed to move to the right edge of the left cylindrical multi-way valve pneumatic chamber 521, and the rear gas is discharged from the first pneumatic tube venting port 508 for decompression recovery, and the lower cylindrical multi-way valve mother liquor interface channel 517 is communicated with the cylindrical multi-way valve outlet channel 519. Considering that the cylindrical multi-way valve element 512 is longer, pneumatic actuation is used, which is more powerful than magnetic actuation.
(6) Mother liquor switch attachment two 6 (fig. 8):
comprises a multi-eccentric rotary manual/automatic dual-purpose valve 601 and a connecting pipeline. The internal structure of the multi-eccentric rotary manual/automatic dual-purpose valve 601 is similar to that of the single-eccentric rotary manual/automatic dual-purpose valve 301, and 8 independent single-eccentric rotary manual/automatic dual-purpose valves 301 are connected in parallel to form a whole. The mother liquor is different in the design of the input, recovery and output pipelines. The 8 multi-bias valve motors 603 independently drive the eccentric rotary valve cores below the inner parts, so that the 8 mother liquor injectors 411 are respectively communicated with the multi-bias valve outlet 607, the multi-bias valve recovery port 608 and the multi-bias valve inlet 609, and mother liquor is respectively pushed to the high-frequency seven-way solenoid valve 701 from the multi-bias valve outlet 610, discharged from the multi-bias valve recovery pipe 611 and pumped into the mother liquor injectors 411 from the multi-bias valve inlet 612. The three communication positions can be realized in a circulating way when the single eccentric rotary valve core rotates in the same direction (clockwise or anticlockwise); by adjusting the rotation direction of the single eccentric rotary valve core, the valve can be rapidly rotated (clockwise or anticlockwise) in any communication mode to reach any next communication position. The multi-bias valve handle 604 is used for manually and independently adjusting the angle of each eccentric rotary valve core in the lower part and is used for switching the input, recovery, output and disconnection of mother liquor.
(7) Flow channel distribution module 7 (fig. 9, 10, 15, 16, 17):
comprises 8 high-frequency seven-way solenoid valves 701, 8 liquid storage rings or quantitative rings and connecting pipelines.
Each high-frequency seven-way solenoid valve 701 independently switches flow paths and is provided with seven interfaces: seven-way valve waste liquid interface 717, seven-way valve mother liquid interface 718, seven-way valve diluent interface 719, seven-way valve purge interface 720, reservoir or dosing ring inlet interface 721, reservoir or dosing ring outlet interface 722, seven-way valve test liquid interface 723.
Seven-way valve waste liquid interface 717 is used to drain excess waste liquid, seven-way valve mother liquid interface 718 is used to input mother liquid, seven-way valve diluent interface 719 is used to input diluent, seven-way valve purge interface 720 is used to input purge air, reservoir or dosing ring inlet interface 721 and reservoir or dosing ring outlet interface 722 are used to mount reservoir or dosing ring, and seven-way valve test liquid interface 723 is used to drain test liquid.
Each high-frequency seven-way solenoid valve 701 is composed of a seven-way valve upper cavity 702 with a smaller upper diameter and a seven-way valve lower cavity 703 with a slightly larger lower diameter. The thickened seven-way valve lower cavity 703 facilitates machining of the inner 7 seven-way valve lower cavity interfaces 711. The seven-way valve lower cavity interface 711 is provided with an inner wire, and each pipeline is screwed into the seven-way valve lower cavity interface 711 through the seven-way valve connector 714 provided with an outer wire, and is respectively and tightly connected with the 7 seven-way valve inner conversion passages 732.
The high frequency seven-way solenoid valve 701 has a seven-way valve cavity 730 extending vertically therethrough and is machined with a vertical seven-way valve cavity recess 731 for mounting a seven-way valve spool 727. The seven-way valve core 727 is a cylinder, and a seven-way valve core fixing edge 726 is arranged in the vertical direction of the periphery and is used for being embedded into a seven-way valve cavity groove 731, so that the seven-way valve core 727 can only vertically move in the seven-way valve cavity 730. The middle part of the seven-way valve core 727 is a seven-way valve core body 735, and the upper end and the lower end of the seven-way valve core 727 are respectively provided with a seven-way valve core magnetic pad 725 which can be attracted by magnetic force, so that the seven-way valve core magnetic pad vertically moves under the action of the magnetic force.
Seven-way valve upper piston 705 and seven-way valve lower piston 709 are respectively arranged above and below a seven-way valve core 727 in the seven-way valve inner cavity 730, and each piston consists of a screw rod with a smaller diameter and a round table with a larger diameter. A seven-way valve upper piston coil 733 is arranged at the bottom of the round table at the lower end of the seven-way valve upper piston 705. A seven-way valve lower piston coil 734 is provided on top of the upper end land of the seven-way valve lower piston 709. After being electrified, the seven-way valve core 727 can be attracted to be clung to the bottom of the round table of the upper piston 705 of the seven-way valve upwards or clung to the top of the round table of the lower piston 709 of the seven-way valve downwards.
The seven-way valve motor 704 is secured to the top of the seven-way valve upper cavity 702 by a seven-way valve motor securing plate 706 and a seven-way valve motor securing aperture 707. The seven-way valve lower motor 708 is fixed to the bottom of the seven-way valve lower chamber 703 by a seven-way valve lower motor fixing plate 710 and a seven-way valve upper motor fixing hole 707. Seven-way valve upper motor 704 drives seven-way valve upper piston 705 to move vertically, and seven-way valve lower motor 708 drives seven-way valve lower piston 709 to move vertically, so that seven-way valve upper piston 705, seven-way valve lower piston 709 and seven-way valve core 727 sandwiched between them can be pushed to a proper position. The seven-way valve core 727 can move up and down at high speed under the action of electromagnetic force and can also be pushed by a piston to move up and down.
The seven-way valve body 735 is pre-machined with 8 layers of mutually independent channels along the vertical direction (fig. 17), and 8 communication modes (seven-way valve positions one to eight) are respectively provided. The open state (seven-way valve core open position) is arranged between each layer of channels, above the uppermost layer of channels and below the lowermost layer of channels. The positions of the seven-way valve core, namely the one-eight valve core and the seven-way valve core, can be switched in millisecond level under the action of electromagnetic force. The positions of the seven-way valve upper piston 705 and the seven-way valve lower piston 709 define the high-speed range of motion of the seven-way valve spool 727. For example: the upper piston and the lower piston can rapidly position the seven-way valve core 727 to the fourth position under the drive of a motor, then the upper piston can be moved to the third position in advance, namely, two spaces of the third position and the fourth position of the seven-way valve core 727 are reserved between the upper piston and the lower piston, and then the seven-way valve core 727 can be subjected to ultra-high-speed switching between the third position and the fourth position through a high-frequency switching circuit coil, so that the ultra-high-speed switching of a flow path is realized, and the extremely accurate required volume is obtained.
The test liquid tube 713 is used for outputting test liquid to the test tube 724, a check head 736 is fixed at the tail end of the test liquid tube 713, the check head 736 is processed into a snake-shaped check head internal channel 737 with a small diameter, the solution in the test liquid tube 713 enters from the check head inlet 738 and is discharged from the check head outlet 739, and the solution in the test tube 724 is prevented from flowing backwards and the solution in the test liquid tube 713 seeps out, so that the test liquid volume is accurate.
When the high-frequency seven-way solenoid valve 701 is installed on the liquid storage ring 712, the internal volume of the liquid storage ring should be larger than the internal volume of the mother liquid injector 411, and when the quantitative ring 712 is installed, the internal volume of the quantitative ring should be smaller than the internal volume of the mother liquid injector 411.
(8) Purge module 8 (fig. 11):
comprising a peristaltic pump 801 and 8 peristaltic tubes 808. Peristaltic pump motor 807 drives extruder 803 into rotation, extruding column 804 pulses peristaltic tube 808, causing internal air flow. The extruder 803 is cylindrical, and the periphery is provided with two semicircular peristaltic tube spacing pincers 802, and the space between peristaltic tube spacing pincers 802 and the extruder 803 is adjustable, and there are three spacing screw 806 in the bottom of every peristaltic tube spacing pincers 802 to screw in and fixed screw hole, and spacing screw 806 can slide in spacing screw adjustment groove 805, can fix the peristaltic tube spacing pincers 802 of top after tightening. Peristaltic pump 801 may be adapted to peristaltic tubing 808 of different thickness and facilitate replacement of peristaltic tubing 808. Each peristaltic tube 808 is individually connected to one high frequency seven-way solenoid valve 701.
2. The liquid preparation method comprises the following steps:
system configuration one: (for a fully functional configuration)
First, the instrument system is connected, and each part is connected by a diluent supply module 1, a diluent switching accessory II 3, a mother liquor supply module 4, a mother liquor switching accessory II 6, a flow path distribution module 7, and a purge module 8: the single-bias valve inlet pipe 312 is inserted into the diluent, the single-bias valve recovery pipe 311 is inserted back into the diluent, the 8 single-bias valve outlets 310 are respectively connected with seven-way valve diluent interfaces 719 of the 8 high-frequency seven-way electromagnetic valves 701, the 8 multi-bias valve inlet pipes 612 are inserted into the mother liquor, the 8 multi-bias valve recovery pipes 611 are respectively inserted back into the 8 mother liquors, the 8 multi-bias valve outlet pipes 610 are respectively connected with seven-way valve mother liquor interfaces 718 of the 8 high-frequency seven-way electromagnetic valves 701, the 8 peristaltic pipes 808 are respectively connected with seven-way valve purge interfaces 720 of the 8 high-frequency seven-way electromagnetic valves 701, the 8 high-frequency seven-way electromagnetic valves 701 are respectively provided with liquid storage rings or quantitative ring inlet interfaces 721 and liquid storage rings or quantitative ring outlet interfaces 722 (the liquid storage ring volumes are slightly larger than the volumes of the sample injectors 411), the 8 seven-way valve liquid supply interfaces 723 of the 8 high-frequency seven-way electromagnetic valves are respectively connected with 8 liquid supply pipes 713, and the 8 test tubes 724 are respectively output to the 8 test tubes 724.
The following 11 functions can be realized:
(1) Precisely measuring a diluent:
diluent supply module 1 and diluent switching accessory II 3 used therein
The single-offset valve core 317 rotates to enable the sample injector 124 to be communicated with the single-offset valve inlet pipe 312, the dilution piston 119 moves downwards, the dilution liquid enters the dilution sample injector 124, then the single-offset valve core 317 rotates to enable the sample injector 124 to be communicated with the single-offset valve recovery pipe 311, the dilution piston 119 moves upwards for a certain stroke precisely, and a certain volume of dilution liquid enters the single-offset valve recovery pipe 311 and is discharged, so that a certain volume of dilution liquid can be measured precisely.
(2) Precisely measuring a mother solution:
mother liquor supply module 4 and mother liquor switching accessory II 6 used therein
The multi-eccentric rotary manual/automatic dual-purpose valve 601 rotates any single eccentric rotary valve core, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve inlet 609, the mother liquor piston 415 moves downwards, the mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve core at the corresponding position rotates again, the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve recovery port 608, the mother liquor piston moves upwards for a certain stroke precisely, a certain volume of mother liquor enters the corresponding multi-eccentric valve recovery pipe 612 and is then discharged, and a certain volume of mother liquor can be measured precisely.
(3) Precisely measuring 2-8 kinds of mother solutions:
mother liquor supply module 4 and mother liquor switching accessory II 6 used therein
The multi-eccentric rotary manual/automatic dual-purpose valve 601 rotates any 2 or all single eccentric rotary valve cores, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve inlet 609, the mother liquor piston 415 moves downwards, the mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve core at the corresponding position rotates again, the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve recovery port 608, the mother liquor piston moves upwards for a certain stroke precisely, and a certain volume of mother liquor enters the corresponding multi-eccentric valve recovery pipe 612 and is then discharged, so that 2-8 precisely measured independent mother liquor are obtained. The corresponding multi-valve recovery tube 612 can also be collected into the same test tube to obtain a precisely measured mixed mother liquor.
(4) Precisely diluting a mother solution:
the diluent supply module 1, the diluent switching attachment 3, the mother liquor supply module 4, the mother liquor switching attachment 6, the flow path distribution module 7, and the purge module 8 are used.
The multi-eccentric rotary manual valve rotates from a single eccentric rotary valve core at any corresponding position in the dual-purpose valve 601, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve inlet 609, the mother liquor piston 415 moves downwards, so that the mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve core at the corresponding position rotates again, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve recovery port 608, meanwhile, the high-frequency seven-way electromagnetic valve 701 at the corresponding position is switched to a position IV (the rest is switched to an open-circuit position), the mother liquor piston moves upwards for a certain stroke precisely, so that a certain volume of mother liquor enters the liquid storage ring, and then the high-frequency seven-way electromagnetic valve 701 at one corresponding position is switched to a position VI (the rest is switched to an open-circuit position). The dilution piston 119 moves upward for a certain stroke precisely, so as to push the dilution liquid to flow from the dilution injector 124 through the corresponding seven-way valve dilution liquid interface 719, the corresponding liquid storage ring or quantitative ring inlet interface 721, the corresponding liquid storage ring or quantitative ring outlet interface 722 and the corresponding seven-way valve test liquid interface 723 in sequence, finally, the corresponding high-frequency seven-way electromagnetic valve 701 is switched to a position seven, the purging module 8 is started, air peristaltic motion is carried out into a pipeline, and residual liquid in the liquid storage ring 712 and the test liquid pipe 713 is blown into the test tube 124 completely, so that the precise dilution liquid of mother liquid is obtained.
(5) Precisely diluting 2-8 kinds of mother solutions:
the diluent supply module 1, the diluent switching attachment 3, the mother liquor supply module 4, the mother liquor switching attachment 6, the flow path distribution module 7, and the purge module 8 are used.
The multi-eccentric rotary manual-automatic dual-purpose valve 601 rotates any 2 or all single eccentric rotary valve cores, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve inlet 609, the mother liquor piston 415 moves downwards, mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve cores at the corresponding positions rotate again, the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve recovery port 608, and meanwhile, the high-frequency seven-way electromagnetic valve 701 at the corresponding position is switched to the position four (the rest is switched to the open-circuit position).
The mother liquor piston precisely moves upwards for a certain stroke to enable a certain volume of mother liquor to enter the liquid storage ring, and each high-frequency seven-way electromagnetic valve 701 in the process can be independently switched between the fourth position and the third position at an ultrahigh speed, so that mother liquor with different volumes enters the respective liquid storage ring. If mother liquor is not required to be recovered, any mother liquor flows into a seven-way valve waste liquid interface 717; if recovery is desired, each of the eccentric rotary valves on the multi-eccentric rotary manual-automatic dual purpose valve 601 can be controlled individually to allow mother liquor to enter the respective multi-eccentric valve recovery pipe 611.
The high frequency seven-way solenoid valve 701 in the corresponding position is then switched to position six (the rest is switched to the off position). Dilution piston 119 moves precisely upward a stroke pushing a volume of diluent from dilution injector 124 through seven-way valve diluent interface 719, reservoir or dosing ring inlet interface 721, reservoir or dosing ring outlet interface 722, and seven-way valve test fluid interface 723, in sequence.
In this process, each high-frequency seven-way solenoid valve 701 can be independently switched between the six position and the five position at ultrahigh speed, so that the diluents with different volumes can enter the respective liquid storage rings.
By individual high-speed switching of each high-frequency seven-way solenoid valve 701, each liquid storage ring can obtain different volumes of mother liquid and diluent. So that each channel can obtain different dilution times and volumes of the test solution.
Finally, the corresponding high-frequency seven-way solenoid valve 701 is switched to the position seven, the purging module 8 is started, air is peristaltic into the pipeline, and residual liquid in the liquid storage ring 712 and the liquid test tube 713 is completely blown into the test tube 124, so that 2-8 independent mother liquid precise dilutions are obtained.
(6) Preparing standard substance standard yeast solution:
the diluent supply module 1, the diluent switching attachment 3, the mother liquor supply module 4, the mother liquor switching attachment 6, the flow path distribution module 7, and the purge module 8 are used.
All multi-eccentric valve inlet pipes 612 are inserted into the same standard solution, any or all single eccentric rotary valve cores in the multi-eccentric rotary manual dual-purpose valve 601 are rotated, so that a mother liquor injector 411 at the corresponding position below is communicated with a corresponding multi-eccentric valve inlet 609, a mother liquor piston 415 moves downwards, mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve core at the corresponding position is rotated again, the mother liquor injector 411 at the corresponding position below is communicated with a corresponding multi-eccentric valve recovery port 608, and meanwhile, a high-frequency seven-way electromagnetic valve 701 at the corresponding position is switched to a position IV (the rest is switched to an open circuit position).
The mother liquor piston precisely moves upwards for a certain stroke to enable a certain volume of mother liquor to enter the liquid storage ring, and each high-frequency seven-way electromagnetic valve 701 in the process can be independently switched between the fourth position and the third position at an ultrahigh speed, so that mother liquor with different volumes enters the respective liquid storage ring. If mother liquor is not required to be recovered, any mother liquor flows into a seven-way valve waste liquid interface 717; if recovery is desired, each of the eccentric rotary valves on the multi-eccentric rotary manual-automatic dual purpose valve 601 can be controlled individually to allow mother liquor to enter the respective multi-eccentric valve recovery pipe 611.
The high frequency seven-way solenoid valve 701 in the corresponding position is then switched to position six (the rest is switched to the off position). Dilution piston 119 moves precisely upward a stroke pushing a volume of diluent from dilution injector 124 through seven-way valve diluent interface 719, reservoir or dosing ring inlet interface 721, reservoir or dosing ring outlet interface 722, and seven-way valve test fluid interface 723, in sequence. In this process, each high-frequency seven-way solenoid valve 701 can be independently switched between the six position and the five position at ultrahigh speed, so that the diluents with different volumes can enter the respective liquid storage rings.
By individual high-speed switching of each high-frequency seven-way solenoid valve 701, each liquid storage ring can obtain different volumes of mother liquid and diluent. Each liquid storage ring corresponds to different concentration points of the standard curve.
Finally, the corresponding high-frequency seven-way solenoid valve 701 is switched to the position seven, the purging module 8 is started, air is peristaltic into the pipeline, and residual liquid in the liquid storage ring 712 and the liquid test tube 713 is completely blown into the test tube 124, so that a plurality of concentration standard curve solutions of one standard product are obtained.
In this embodiment, 8 concentration standard yeast solutions can be prepared simultaneously at a time, and if the number of channels of the mother liquor supply module 4, the mother liquor switching accessory two 6 and the flow path distribution module 7 is increased, more than 8 concentration standard yeast solutions can be prepared at a time.
(7) Preparing 2-8 mixed standard solutions:
the diluent supply module 1, the diluent switching attachment 3, the mother liquor supply module 4, the mother liquor switching attachment 6, the flow path distribution module 7, and the purge module 8 are used.
The multi-eccentric rotary manual valve 601 is characterized in that a multi-eccentric valve inlet pipe 612 is inserted into 2-8 different standard substance solutions, any 2 or all single eccentric rotary valve cores in the multi-eccentric rotary manual valve 601 are rotated, so that a mother liquor injector 411 at the corresponding position below is communicated with a corresponding multi-eccentric valve inlet 609, a mother liquor piston 415 moves downwards, mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve core at the corresponding position is rotated again, the mother liquor injector 411 at the corresponding position below is communicated with a corresponding multi-eccentric valve recovery port 608, and meanwhile, a high-frequency seven-way electromagnetic valve 701 at the corresponding position is switched to a position IV (the rest is switched to an open-circuit position).
The mother liquor piston precisely moves upwards for a certain stroke to enable a certain volume of mother liquor to enter the liquid storage ring, and each high-frequency seven-way electromagnetic valve 701 in the process can be independently switched between the fourth position and the third position at an ultrahigh speed, so that mother liquor with different volumes enters the respective liquid storage ring. If mother liquor is not required to be recovered, any mother liquor flows into a seven-way valve waste liquid interface 717; if recovery is desired, each of the eccentric rotary valves on the multi-eccentric rotary manual-automatic dual purpose valve 601 can be controlled individually to allow mother liquor to enter the respective multi-eccentric valve recovery pipe 611.
The high frequency seven-way solenoid valve 701 in the corresponding position is then switched to position six (the rest is switched to the off position). Dilution piston 119 moves precisely upward a stroke pushing a volume of diluent from dilution injector 124 through seven-way valve diluent interface 719, reservoir or dosing ring inlet interface 721, reservoir or dosing ring outlet interface 722, and seven-way valve test fluid interface 723, in sequence. In this process, each high-frequency seven-way solenoid valve 701 can be independently switched between the six position and the five position at ultrahigh speed, so that the diluents with different volumes can enter the respective liquid storage rings.
By individual high-speed switching of each high-frequency seven-way solenoid valve 701, each liquid storage ring can obtain different volumes of mother liquid and diluent. Each reservoir ring corresponds to the respective required amount of different standards in the mixed standard solution, and the volume of dilution liquid in the reservoir ring and the required amount of dilution liquid for that portion of mixed standard solution.
Finally, the corresponding high-frequency seven-way solenoid valve 701 is switched to the position seven, the purging module 8 is started, air is peristaltic into the pipeline, and residual liquid in the liquid storage ring 712 and the liquid to be tested 713 is blown into the same test tube 124, so that mixed solution of 2-8 standard substances is obtained.
In this embodiment, 8 concentration standard curve solutions can be simultaneously prepared at a time, and if the number of channels of the mother liquor supply module 4, the mother liquor switching accessory II 6 and the flow path distribution module 7 is increased, more than 8 standard solutions can be prepared at a time.
(8) And (3) mother liquor washing:
a mother liquid supply module 4, a mother liquid switching attachment II 6, and a flow path distribution module 7 are used.
The multi-eccentric rotary manual self-dual-purpose valve 601 rotates any several or all single eccentric rotary valve cores, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve inlet 609, the mother liquor piston 415 moves downwards, the mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve core at the corresponding position rotates again, the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve recovery port 608, the mother liquor piston moves upwards, the mother liquor reaches the seven-way valve mother liquor interface 718 of the high-frequency seven-way solenoid valve 701, the high-frequency seven-way solenoid valve 701 is firstly switched to the position III, the mother liquor enters a waste liquor pipe, the pipeline is cleaned, bubbles are removed, and when no bubbles are detected by the multi-connected bubble sensor 421 for a certain time, the high-frequency seven-way solenoid valve 701 is switched to the position IV, and the mother liquor can enter a liquid storage ring.
(9) Rinsing with a diluent:
the diluent supply module 1, the diluent switching accessory two 3, and the flow path distribution module 7 are used.
The single bias valve core 317 rotates to enable the injector 124 to be communicated with the single bias valve inlet pipe 312, the dilution piston 119 moves downwards, the dilution liquid enters the dilution injector 124, then the single bias valve core 317 rotates to enable the injector 124 to be communicated with the 8 single bias valve outlets 310, the dilution piston 119 moves upwards to enable the dilution liquid to reach the seven-way valve dilution liquid interface 719 of the high-frequency seven-way electromagnetic valve 701, the high-frequency seven-way electromagnetic valve 701 is firstly switched to the position five, the dilution liquid enters the waste liquid pipe, the pipeline is cleaned and bubbles are removed, and when no bubbles are detected by the multi-bubble sensor 421 for a certain time, the high-frequency seven-way electromagnetic valve 701 is switched to the position six, and the dilution liquid can enter the liquid storage ring.
(10) Pre-rinsing the liquid storage ring:
the program may be run each time it is powered on.
The diluent supply module 1, the diluent switching attachment two 3, the flow path distribution module 7, and the purge module 8 are used.
Single bias valve inlet tube 312 is inserted into the diluent, single bias valve core 317 is rotated, injector 124 is connected to single bias valve inlet tube 312, dilution piston 119 is moved downward, and the diluent enters dilution injector 124. Subsequently, the single bias valve core 317 rotates to enable the injector 124 to communicate with the 8 single bias valve outlets 310, meanwhile, the high-frequency seven-way electromagnetic valve 701 is switched to the first position, the dilution piston 119 moves upwards to push the diluent to flow from the dilution injector 124 to the seven-way valve diluent interface 719, the reservoir ring or dosing ring inlet interface 721, the reservoir ring or dosing ring outlet interface 722 and the seven-way valve waste liquid interface 717 in sequence, so that the diluent washes the reservoir ring, then the high-frequency seven-way electromagnetic valve 701 is switched to the second position, the purging module 8 is started, air is peristaltic into the pipeline, and residual diluent in the reservoir ring 712 and the valve core is blown clean.
(11) And (3) cleaning a system:
the program may be run before each shutdown.
The diluent supply module 1, the diluent switching attachment 3, the mother liquor supply module 4, the mother liquor switching attachment 6, the flow path distribution module 7, and the purge module 8 are used.
The single-bias valve inlet pipe 312 and the 8 multi-bias valve inlet pipes 612 are inserted into the cleaning liquid, all single-bias rotary valve cores in the multi-bias rotary manual-automatic dual-purpose valve 601 rotate, so that the mother liquid injector 411 below is communicated with the multi-bias valve inlet 609, and the mother liquid piston 415 moves downwards, so that the cleaning liquid enters the mother liquid injector 411.
The single bias valve core 317 rotates to allow the injector 124 to communicate with the single bias valve inlet tube 312, the dilution piston 119 moves downward, and the cleaning fluid enters the dilution injector 124.
The mother liquor piston 415 and the dilution piston 119 move upward at the same time, and the high-frequency seven-way electromagnetic valve 701 is sequentially switched to the first, third, fourth, fifth, sixth and eighth positions, so that the cleaning liquid enters each prefabricated passage inside the high-frequency seven-way electromagnetic valve 701, passes through the liquid storage ring, and then passes through the liquid test tube 713 to be discharged. After a certain time of operation, the high-frequency seven-way solenoid valve 701 is switched to a position seven, the purging module 8 is started, air peristaltic motion enters the pipeline, and residual cleaning liquid in the liquid storage ring 712 and the test liquid pipe 713 is purged.
And (3) system configuration II: (for easy and quick configuration, cost can be reduced)
The instrument system was reconnected using the diluent supply module 1, the diluent switching accessory 1, the mother liquor supply module 4, the mother liquor switching accessory 5, the flow path distribution module 7, and the purge module 8, and the respective parts were connected: the method comprises the steps of inserting a T-valve diluent inlet pipe 207 into diluent, respectively connecting 8 diluent outlet pipes 211 with seven-way valve diluent interfaces 719 of 8 high-frequency seven-way electromagnetic valves 701, respectively inserting 8 mother liquor inlet pipes 417 into 8 mother liquors, respectively connecting 8 mother liquor outlet pipes 422 with seven-way valve mother liquor interfaces 718 of 8 high-frequency seven-way electromagnetic valves 701, respectively connecting 8 peristaltic pipes 808 with seven-way valve purge interfaces 720 of 8 high-frequency seven-way electromagnetic valves 701, respectively installing 8 quantitative rings (the volume of the quantitative rings is smaller than that of a mother liquor injector 411) on a liquid storage ring or a quantitative ring inlet interface 721 and a liquid storage ring or a quantitative ring outlet interface 722 of the 8 high-frequency seven-way electromagnetic valves 701, respectively connecting seven-way valve test liquid interfaces 723 of the 8 high-frequency seven-way electromagnetic valves 701 with 8 test liquid pipes 713, respectively outputting the 8 test tubes 724.
The following 6 functions can be realized:
(1) 8 kinds of mother liquor are precisely and rapidly diluted:
the diluent supply module 1, the diluent switching unit 1, the mother liquor supply module 4, the mother liquor switching unit 5, the flow path distribution module 7, and the purge module 8 are used.
The 8 mother liquor inlet pipes 417 are inserted into the 8 mother liquors, respectively. The cylindrical multi-way manual dual-purpose valve 501 has its cylindrical multi-way valve spool 512 switched to the right (fig. 14) with the lower mother liquor injector 411 in communication with the corresponding cylindrical multi-way valve spool inlet channel 513 and the mother liquor piston 415 moved down to bring mother liquor into the mother liquor injector 411, then the cylindrical multi-way manual dual-purpose valve 501 has its cylindrical multi-way valve spool 512 switched to the left with the lower mother liquor injector 411 in communication with the corresponding cylindrical multi-way valve spool outlet channel 514 and the high frequency seven-way solenoid valve 701 switched to position four.
The mother liquor piston runs up fast (faster than the system is configured with a volume controlled by the dosing ring) so that mother liquor enters the dosing ring and excess mother liquor directly enters the seven-way valve waste liquid interface 717. Then high frequency seven-way solenoid valve 701 is switched to position six, diluting piston 119 is moved upward precisely by a certain stroke, forcing a volume of diluent to flow from diluting injector 124 sequentially through seven-way valve diluent interface 719, reservoir or dosing ring inlet interface 721, reservoir or dosing ring outlet interface 722, and seven-way valve test fluid interface 723.
In this process, each high-frequency seven-way solenoid valve 701 can be independently switched between the six position and the five position at ultrahigh speed, so that the diluents with different volumes can enter the respective liquid storage rings.
By individual high speed switching of each high frequency seven-way solenoid valve 701, a different volume of diluent can be obtained for each dosing ring. So that each channel can obtain different dilution times and volumes of the test solution.
Finally, the corresponding high-frequency seven-way solenoid valve 701 is switched to the position seven, the purging module 8 is started, air is peristaltic into the pipeline, and all residual liquid in the quantitative ring 712 and the test liquid pipe 713 is blown into the test tube 124, so that 8 independent mother liquid precise dilutions are obtained.
(2) Rapidly preparing standard substance standard yeast solution:
the diluent supply module 1, the diluent switching unit 1, the mother liquor supply module 4, the mother liquor switching unit 5, the flow path distribution module 7, and the purge module 8 are used.
The 8 mother liquors were inserted into the same standard solution via pipe 417 and the remainder was subjected to precise rapid dilution as described above for "(1) 8 mother liquors. 8 standard solutions of standard substance were obtained.
In this embodiment, 8 concentration standard yeast solutions can be prepared simultaneously at a time, and if the number of channels of the mother liquor supply module 4, the mother liquor switching accessory one 5 and the flow path distribution module 7 is increased, more than 8 concentration standard yeast solutions can be prepared at a time.
(3) And (3) mother liquor washing:
A mother liquor supply module 4, a mother liquor switching attachment 5, and a flow path distribution module 7 are used.
The cylindrical multi-way manual dual-purpose valve 501 has its cylindrical multi-way valve spool 512 switched to the right (fig. 14) with the lower mother liquor injector 411 in communication with the corresponding cylindrical multi-way valve spool inlet channel 513 and the mother liquor piston 415 moved downward with mother liquor entering the mother liquor injector 411, and then the cylindrical multi-way manual dual-purpose valve 501 has its cylindrical multi-way valve spool 512 switched to the left with the lower mother liquor injector 411 in communication with the corresponding cylindrical multi-way valve spool outlet channel 514.
The mother liquor piston moves upwards, so that the mother liquor reaches a seven-way valve mother liquor interface 718 of the high-frequency seven-way electromagnetic valve 701, the high-frequency seven-way electromagnetic valve 701 is firstly switched to a position III, the mother liquor enters a waste liquor pipe, a pipeline is cleaned and bubbles are removed, when a multi-connected bubble sensor 421 detects no bubbles for a certain time, the high-frequency seven-way electromagnetic valve 701 is switched to a position IV, and the mother liquor can enter a quantitative ring.
(4) Rinsing with a diluent:
the diluent supply module 1, the diluent switching accessory 1, and the flow path distribution module 7 are used.
The T-shaped valve core is made to enter the injector channel 220 to communicate the T-shaped valve dilution interface channel 223 and the dilution liquid separating cavity inlet channel 224, the dilution piston 119 moves downwards, the dilution liquid enters the dilution injector 124, then the T-shaped valve core is made to exit the injector channel 221 to communicate the T-shaped valve dilution interface channel 223 and the dilution liquid inlet channel 227, the dilution piston 119 moves upwards, the dilution liquid reaches the seven-way valve dilution liquid interface 719 of the high-frequency seven-way electromagnetic valve 701, the high-frequency seven-way electromagnetic valve 701 is firstly switched to the position five, the dilution liquid enters the waste liquid pipe, the pipeline is cleaned and bubbles are removed, and when no bubble is detected by the multi-bubble sensor 421 for a certain time, the high-frequency seven-way electromagnetic valve 701 is switched to the position six, and the dilution liquid can enter the dosing ring.
(5) Quantitative ring pre-rinse:
the program may be run each time it is powered on.
The diluent supply module 1, the diluent switching accessory 1, the flow path distribution module 7, and the purge module 8 are used.
The T-valve inlet injector passage 220 communicates with the T-valve dilution interface passage 223 and the dilution liquid separation chamber inlet passage 224, the dilution piston 119 moves downward, and the dilution liquid enters the dilution injector 124. Then, the T-shaped valve core outlet injector channel 221 is communicated with the T-shaped valve dilution interface channel 223 and the diluent inlet channel 227, meanwhile, the high-frequency seven-way electromagnetic valve 701 is switched to the first position, the diluent piston 119 moves upwards to push diluent to flow through the seven-way valve diluent interface 719, the liquid storage ring or dosing ring inlet interface 721, the liquid storage ring or dosing ring outlet interface 722 and the seven-way valve waste liquid interface 717 in sequence from the dilution injector 124, so that the diluent washes the dosing ring, then the high-frequency seven-way electromagnetic valve 701 is switched to the second position, the purging module 8 is started, air is peristaltic into the pipeline, and residual diluent in the dosing ring 712 and the valve core is blown clean.
(6) And (3) cleaning a system:
the program may be run before each shutdown.
The diluent supply module 1, the diluent switching unit 1, the mother liquor supply module 4, the mother liquor switching unit 5, the flow path distribution module 7, and the purge module 8 are used.
The T-valve diluent inlet pipe 207 and the 8 mother liquor inlet pipes 417 are inserted into the cleaning liquid, and the cylindrical multi-way valve core 512 of the cylindrical multi-way manual-automatic dual-purpose valve 501 is switched to the right position (fig. 14), so that the lower mother liquor injector 411 is communicated with the corresponding cylindrical multi-way valve core inlet channel 513, and the mother liquor piston 415 moves downwards, so that the cleaning liquid enters the mother liquor injector 411.
The T-valve inlet injector passage 220 communicates with the T-valve dilution interface passage 223 and the dilution liquid separation chamber inlet passage 224, the dilution piston 119 moves downward, and the dilution liquid enters the dilution injector 124.
The mother liquor piston 415 and the dilution piston 119 move upward at the same time, and the high-frequency seven-way electromagnetic valve 701 is sequentially switched to the first, third, fourth, fifth, sixth and eighth positions, so that the cleaning liquid enters each prefabricated passage inside the high-frequency seven-way electromagnetic valve 701, passes through the dosing ring, and then is discharged through the liquid sample tube 713. After a certain period of operation, the high-frequency seven-way solenoid valve 701 is switched to the position seven, the purging module 8 is started, air peristaltic motion enters the pipeline, and residual cleaning liquid in the dosing ring 712 and the test liquid pipe 713 is purged.
System configuration three (for preparing a maximum of 8 mother liquors containing internal standards at a time):
the instrument system was reconnected, and each part was connected using 2 mother liquor supply modules 4, 2 mother liquor switching accessories two 6, 1 flow path distribution module 7, 1 purge module 8: the 8 multi-valve inlet pipes 612 of the first mother liquor switching accessory two 6 are inserted into the 8 mother liquors, and the 8 multi-valve inlet pipes 612 of the second mother liquor switching accessory two 6 are inserted into the internal standard solution. The 8 multi-bias valve outlet pipes 610 of the first mother liquor switching accessory II 6 are respectively connected with the seven-way valve mother liquor interfaces 718 of the first 8 high-frequency seven-way electromagnetic valves 701, and the 8 multi-bias valve outlet pipes 610 of the second mother liquor switching accessory II 6 are respectively connected with the seven-way valve diluent interfaces 719 of the last 8 high-frequency seven-way electromagnetic valves 701.
The 8 peristaltic tubes 808 are respectively connected with seven-way valve purging interfaces 720 of the 8 high-frequency seven-way electromagnetic valves 701, the liquid storage ring or quantitative ring inlet interface 721 and the liquid storage ring or quantitative ring outlet interface 722 of the 8 high-frequency seven-way electromagnetic valves 701 are respectively provided with 8 liquid storage rings (the volume of the liquid storage rings is slightly larger than that of the mother liquid injector 411), and seven-way valve test liquid interfaces 723 of the 8 high-frequency seven-way electromagnetic valves 701 are respectively connected with 8 test liquid tubes 713.
The following 4 functions can be realized:
(1) 1 to 8 mother solutions containing internal standard are prepared:
the first multi-eccentric rotary valve rotates from any 1 or all single eccentric rotary valve cores in the dual-purpose valve 601, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve inlet 609, the mother liquor piston 415 moves downwards, so that mother liquor enters the mother liquor injector 411, then the single eccentric rotary valve cores at the corresponding positions rotate again, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve recovery port 608, and meanwhile, the high-frequency seven-way electromagnetic valve 701 at the corresponding position is switched to the position four (the rest is switched to the open circuit position).
The mother liquor piston precisely moves upwards for a certain stroke to enable a certain volume of mother liquor to enter the liquid storage ring, and each high-frequency seven-way electromagnetic valve 701 in the process can be independently switched between the fourth position and the third position at an ultrahigh speed, so that mother liquor with different volumes enters the respective liquid storage ring. If mother liquor is not required to be recovered, any mother liquor flows into a seven-way valve waste liquid interface 717; if recovery is desired, each of the eccentric rotary valves on the multi-eccentric rotary manual-automatic dual purpose valve 601 can be controlled individually to allow mother liquor to enter the respective multi-eccentric valve recovery pipe 611.
The high frequency seven-way solenoid valve 701 in the corresponding position is then switched to position six (the rest is switched to the off position).
The second multi-eccentric rotary manual/automatic dual-purpose valve 601 operates as described above to allow the internal standard solution in the corresponding path of the mother liquor injector 411 to enter the reservoir ring from the seven-way valve diluent interface 719.
In the process, each high-frequency seven-way electromagnetic valve 701 can be independently switched between the six position and the five position at ultrahigh speed, so that internal standard solutions with different volumes can enter respective liquid storage rings.
By means of the independent high-speed switching of each high-frequency seven-way electromagnetic valve 701, each liquid storage ring can obtain mother liquid and internal standard solution with different volumes.
Finally, the high-frequency seven-way solenoid valve 701 is switched to the position seven, the purging module 8 is started, air is peristaltic into the pipeline, and residual liquid in the liquid storage ring 712 and the liquid to be tested 713 is completely blown into the test tube 124, so that 1-8 independent mother liquids containing internal standards are obtained.
(2) And (3) mother liquor washing: and the same system configuration is adopted.
(3) Quantitative ring pre-rinse: and the same system configuration is adopted.
(4) And (3) cleaning a system: and the same system configuration is adopted.
System configuration four (for precise measurement of up to 16 mother liquors):
reconnecting the instrument system, and connecting the parts by adopting 2 mother liquor supply modules 4 and 2 mother liquor switching accessories II 6: the 16 multi-valve inlet pipes 612 are inserted into the 16 mother liquors, and the 16 multi-valve recovery pipes 611 are inserted into the 16 test tubes 724, respectively.
The following functions can be realized:
precisely measuring 9-16 kinds of mother solutions:
the two multi-eccentric rotary manual-automatic dual-purpose valves 601 rotate any 9-16 single-eccentric rotary valve cores, so that the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve inlet 609, the mother liquor piston 415 moves downwards, so that the mother liquor enters the mother liquor injector 411, then the single-eccentric rotary valve core at the corresponding position rotates again, the mother liquor injector 411 at the corresponding position below is communicated with the corresponding multi-eccentric valve recovery port 608, the mother liquor piston moves upwards for a certain stroke precisely, and a certain volume of mother liquor enters the corresponding multi-eccentric valve recovery pipe 612 and is then discharged, and 9-16 mother liquor can be measured precisely.
Claims (10)
1. An inspection pretreatment multichannel liquid distribution system, comprising:
a diluent supply module for supplying a diluent;
the diluent switching accessory I comprises a first multichannel valve; the diluent switching accessory I is connected with the diluent supply module and is used for uniformly distributing the supplied diluent to all channels of the first multi-channel valve; enabling all channels of the first multi-channel valve to obtain diluent simultaneously and uniformly;
the diluent switching accessory II comprises a second multichannel valve; the diluent switching accessory I is connected with the diluent supply module and is used for uniformly distributing the supplied diluent to all channels of the second multi-channel valve; all channels of the second multi-channel valve can simultaneously and uniformly obtain diluent, and the diluent is used for realizing independent precise measurement and recovery of the diluent;
A mother liquor supply module for supplying mother liquor;
the mother liquor switching accessory I comprises a third multichannel valve and is used for accessing various mother liquor of the mother liquor supply module;
the mother liquor switching accessory II comprises a fourth multichannel valve and is used for accessing various mother liquor of the mother liquor supply module to realize independent precise measurement and recovery of the mother liquor;
and one end of the flow path distribution module is connected with the first diluent switching accessory or the second diluent switching accessory, and the other end of the flow path distribution module is connected with the first mother liquor switching accessory or the second mother liquor switching accessory.
2. The test preconditioning multichannel liquid dispensing system of claim 1, wherein said first multichannel valve is a T-valve (201) comprising a T-valve core (218); the T-shaped valve core is internally provided with a T-shaped valve core inlet sampler channel (220), a T-shaped valve core outlet sampler channel (221), a T-shaped valve dilution interface (222), a T-shaped valve dilution interface channel (223), a dilution liquid separation cavity inlet channel (224), a dilution liquid separation cavity central channel (225), a liquid separation cavity L channel (226) and a T-shaped valve outlet (206); the T-shaped valve core inlet injector channel (220) is communicated with the T-shaped valve dilution interface channel (223) and the dilution liquid distribution cavity inlet channel (224), and the T-shaped valve core outlet injector channel (221) is communicated with the T-shaped valve dilution interface channel (223) and the dilution liquid inlet channel (227); a center channel (225) of the dilution liquid separating cavity is communicated above the inlet channel (224) of the dilution liquid separating cavity; the center channel (225) of the dilution liquid separating cavity is communicated with a plurality of L channels (226) of the dilution liquid separating cavity, the inner diameters of the L channels (226) of the dilution liquid separating cavity are consistent, the angles are average, and the uniform flow distribution is ensured.
3. The inspection preconditioning multichannel liquid dispensing system of claim 1, wherein said second multichannel valve is a single off-center rotary manual-automatic dual-purpose valve (301), comprising a single off-center valve core (317); the single-bias valve core (317) is provided with a single-bias valve dilution interface channel (320), three independent L-shaped channels and three inlet channels, wherein the three inlet channels are a single-bias valve core distribution main channel (303), a single-bias valve recovery port (304) and a single-bias valve inlet (305) respectively; the three independent L-shaped channels are respectively used for being communicated with a single-bias valve dilution interface channel (320) and one of the three inlet channels, can be circularly communicated with the single-bias valve dilution interface channel (320) at the eccentric position of the bottom of the single-bias valve core (317) under the driving of the single-bias valve motor (307), and are sequentially circularly communicated with a single-bias valve core distribution main channel (303), a single-bias valve recovery port (304) or a single-bias valve inlet (305).
4. The inspection preconditioning multichannel liquid dispensing system of claim 1, wherein said third multichannel valve is a cylindrical multipass manual-automatic dual-purpose valve (501), comprising a cylindrical multipass valve core (512); the cylindrical multi-way valve core (512) is internally provided with a plurality of mutually independent cylindrical multi-way valve core inlet channels (513) and a plurality of cylindrical multi-way valve core outlet channels (514), and the number of the multi-way valve core outlet channels (514) is consistent with that of the cylindrical multi-way valve core inlet channels (513); the cylindrical multi-way valve core inlet channel (513) is communicated with the cylindrical multi-way valve mother liquor interface channel (517) and the cylindrical multi-way valve inlet channel (518), and the cylindrical multi-way valve core outlet channel (514) is communicated with the cylindrical multi-way valve mother liquor interface channel (517) and the cylindrical multi-way valve outlet channel (519).
5. A test preconditioning multichannel dispensing system as claimed in claim 3, characterized in that said fourth multichannel valve is a multi-eccentric rotary manual-automatic dual-purpose valve (601) comprising a plurality of independent single-eccentric rotary manual-automatic dual-purpose valves (301) in parallel.
6. An inspection pretreatment multichannel liquid distribution system according to any of the claims 1-5, characterized in that said flow path distribution module (7) comprises 8 high frequency seven-way solenoid valves (701), 8 reservoir or dosing rings and connecting lines; each high-frequency seven-way electromagnetic valve (701) is independently switched with a flow path, and seven interfaces are respectively a seven-way valve waste liquid interface (717), a seven-way valve mother liquid interface (718), a seven-way valve diluent interface (719), a seven-way valve purging interface (720), a liquid storage ring or quantitative ring inlet interface (721), a liquid storage ring or quantitative ring outlet interface (722) and a seven-way valve test liquid interface (723); the seven-way valve waste liquid interface (717) is used for discharging redundant waste liquid, the seven-way valve mother liquid interface (718) is used for inputting mother liquid, the seven-way valve diluent interface (719) is used for inputting diluent, the seven-way valve purge interface (720) is used for inputting purge air, the liquid storage ring or quantitative ring inlet interface (721) and the liquid storage ring or quantitative ring outlet interface (722) are used for installing a liquid storage ring or quantitative ring, and the seven-way valve test liquid interface (723) is used for discharging test liquid.
7. The inspection pretreatment multichannel liquid dispensing system of claim 6, characterized in that each high frequency seven-way solenoid valve (701) is comprised of a seven-way valve upper cavity (702) with a smaller upper diameter and a seven-way valve lower cavity (703) with a slightly larger lower diameter; 7 seven-way valve lower cavity interfaces (711) are processed in the seven-way valve lower cavity (703); an inner wire is processed on the seven-way valve lower cavity interface (711), and each pipeline is screwed into the seven-way valve lower cavity interface (711) through a seven-way valve joint (714) processed with an outer wire and is respectively and tightly connected with 7 seven-way valve conversion passages (732);
the high-frequency seven-way electromagnetic valve (701) is internally provided with a seven-way valve cavity (730) penetrating up and down, and is processed with a vertical seven-way valve cavity groove (731) for installing a seven-way valve core (727); the seven-way valve core (727) is a cylinder, and a seven-way valve core fixing edge (726) is arranged in the vertical direction of the periphery and is used for being embedded into a seven-way valve cavity groove (731), so that the seven-way valve core (727) can only vertically move in the seven-way valve cavity (730); the middle part of the seven-way valve core (727) is a seven-way valve core body (735), and the upper end and the lower end of the seven-way valve core are respectively provided with a seven-way valve core magnetic pad (725) which can be attracted by magnetic force, so that the seven-way valve core moves vertically under the action of the magnetic force;
A seven-way valve upper piston (705) and a seven-way valve lower piston (709) are respectively arranged above and below a seven-way valve core (727) in the seven-way valve inner cavity (730), and both pistons are provided with a screw rod with a smaller diameter and a round platform with a larger diameter; a seven-way valve upper piston coil (733) is arranged at the bottom of the round table at the lower end of the seven-way valve upper piston (705); a seven-way valve lower piston coil (734) is arranged at the top of the round table at the upper end of the seven-way valve lower piston (709); after being electrified, the seven-way valve core (727) can be attracted to be clung to the bottom of a truncated cone of the upper piston (705) of the seven-way valve upwards or clung to the top of a truncated cone of the lower piston (709) of the seven-way valve downwards;
the seven-way valve upper motor (704) is fixed at the top of the seven-way valve upper cavity (702) through a seven-way valve upper motor fixing plate (706) and a seven-way valve upper motor fixing hole (707); the seven-way valve lower motor (708) is fixed at the bottom of the seven-way valve lower cavity (703) through a seven-way valve lower motor fixing plate (710) and a seven-way valve upper motor fixing hole (707); the seven-way valve upper motor (704) drives the seven-way valve upper piston (705) to vertically move, the seven-way valve lower motor (708) drives the seven-way valve lower piston (709) to vertically move, and the seven-way valve upper piston (705), the seven-way valve lower piston (709) and the seven-way valve core (727) clamped in the middle can be pushed to a proper position, so that the seven-way valve core (727) can move up and down at a high speed under the action of electromagnetic force and can also be pushed to move up and down by the pistons;
The test liquid pipe (713) is used for outputting test liquid to the test tube (724), a check head (736) is fixed at the tail end of the test liquid pipe (713), the check head (736) is processed into a snake-shaped check head internal channel (737) with a small diameter, the solution in the test liquid pipe (713) enters from the check head inlet (738) and is discharged from the check head outlet (739) and is used for preventing the solution in the test tube (724) from flowing backwards and the solution in the test liquid pipe (713) from exuding, so that the test liquid volume is accurate;
when the high-frequency seven-way electromagnetic valve (701) is used for installing the liquid storage ring (712), the internal volume of the liquid storage ring is larger than the internal volume of the mother liquid injector (411), and when the quantitative ring (712) is installed, the internal volume of the quantitative ring is smaller than the internal volume of the mother liquid injector (411).
8. An inspection pretreatment multichannel liquid distribution system according to any of claims 1 to 5, further comprising a purge module coupled to the flow distribution module for cleaning the flow distribution module piping.
9. A test preconditioning multichannel dispensing system according to claim 8, characterized in that the purge module comprises a peristaltic pump (801) and 8 peristaltic tubes (808); a peristaltic pump motor (807) drives a squeezer (803) to rotate, and a squeezing column (804) is used for pulse squeezing a peristaltic tube (808) so as to enable internal air to flow; the extruder (803) is cylindrical, two semicircular peristaltic tube limiting clamps (802) are arranged on the periphery of the extruder, and a gap between the peristaltic tube limiting clamps (802) and the extruder (803) is adjustable; three limit screws (806) are arranged at the bottom of each peristaltic tube limit clamp (802) and can be screwed into and fixed with screw holes, the limit screws (806) can slide in limit screw adjusting grooves (805), and the peristaltic tube limit clamp (802) above can be fixed after being screwed up, so that the peristaltic pump (801) can be suitable for peristaltic tubes (808) with different thicknesses, and the peristaltic tubes (808) can be replaced conveniently; each peristaltic tube (808) is independently communicated with a high-frequency seven-way electromagnetic valve (701).
10. A method of dispensing liquid based on any one of claims 1-9 for testing a preconditioning multichannel liquid dispensing system, comprising:
forming a liquid composition, namely, a first, a second, a third or a fourth liquid composition, wherein:
the first liquid separation configuration is as follows: a diluent supply module (1), a diluent switching accessory II (3), a mother liquor supply module (4), a mother liquor switching accessory II (6) and a flow path distribution module 7 are adopted;
the liquid separation configuration II comprises: a diluent supply module (1), a diluent switching accessory I (2), a mother liquor supply module (4), a mother liquor switching accessory I (5) and a flow path distribution module (7) are adopted;
the liquid separation configuration III comprises: 2 mother liquor supply modules (4), 2 mother liquor switching accessories (6) and 1 flow path distribution module (7) are adopted;
the liquid separation configuration is four: 2 mother liquor supply modules (4) and 2 mother liquor switching accessories (6) are adopted;
the liquid separation configuration one is utilized to carry out the following functional operations:
precisely measuring a diluent;
precisely measuring a mother solution;
precisely measuring 2-8 kinds of mother liquor;
precisely diluting a mother solution;
precisely diluting 2-8 kinds of mother liquor;
preparing a standard substance standard yeast solution;
preparing 2-8 mixed standard solutions;
rinsing mother liquor;
Rinsing with a diluent;
pre-rinsing the liquid storage ring;
and (3) carrying out the following functional operations by utilizing the liquid separation configuration II:
precise and rapid dilution of mother solution;
preparing a standard substance standard yeast solution;
rinsing mother liquor;
rinsing with a diluent;
pre-rinsing the quantitative ring;
and (3) performing the following functional operations by utilizing the liquid separation configuration III:
1 to 8 mother solutions containing internal standard are prepared:
rinsing mother liquor;
pre-rinsing the quantitative ring;
the liquid separation configuration is utilized to carry out the following functional operations:
precisely measuring 9-16 kinds of mother liquor.
Priority Applications (1)
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CN202311492427.3A CN117582834A (en) | 2023-11-10 | 2023-11-10 | Inspection pretreatment multichannel liquid preparation system and liquid preparation method |
Applications Claiming Priority (1)
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