CN117482189A - Hair growth and hair loss prevention application of kaempferia galanga essential oil - Google Patents
Hair growth and hair loss prevention application of kaempferia galanga essential oil Download PDFInfo
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- CN117482189A CN117482189A CN202311769331.7A CN202311769331A CN117482189A CN 117482189 A CN117482189 A CN 117482189A CN 202311769331 A CN202311769331 A CN 202311769331A CN 117482189 A CN117482189 A CN 117482189A
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- hair
- essential oil
- hair growth
- kaempferia galanga
- kaempferia
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- 229930006737 car-3-ene Natural products 0.000 description 1
- 239000001569 carbon dioxide Substances 0.000 description 1
- 229910002092 carbon dioxide Inorganic materials 0.000 description 1
- 150000001851 cinnamic acid derivatives Chemical class 0.000 description 1
- 230000004087 circulation Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 230000003749 cleanliness Effects 0.000 description 1
- MRUAUOIMASANKQ-UHFFFAOYSA-N cocamidopropyl betaine Chemical compound CCCCCCCCCCCC(=O)NCCC[N+](C)(C)CC([O-])=O MRUAUOIMASANKQ-UHFFFAOYSA-N 0.000 description 1
- 229940073507 cocamidopropyl betaine Drugs 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000002285 corn oil Substances 0.000 description 1
- 235000005687 corn oil Nutrition 0.000 description 1
- 238000005536 corrosion prevention Methods 0.000 description 1
- 239000002537 cosmetic Substances 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
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- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000002222 downregulating effect Effects 0.000 description 1
- 239000003995 emulsifying agent Substances 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 230000006353 environmental stress Effects 0.000 description 1
- 231100000321 erythema Toxicity 0.000 description 1
- DHNGCHLFKUPGPX-UHFFFAOYSA-N ethyl 4-methoxycinnamate Natural products CCOC(=O)C=CC1=CC=C(OC)C=C1 DHNGCHLFKUPGPX-UHFFFAOYSA-N 0.000 description 1
- XTZZULGXHUQOEN-UHFFFAOYSA-N ethyl p-methoxycinnamate Natural products CCOC1=CC=C(C=CC(=O)OC)C=C1 XTZZULGXHUQOEN-UHFFFAOYSA-N 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 230000003721 exogen phase Effects 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- 229960004039 finasteride Drugs 0.000 description 1
- DBEPLOCGEIEOCV-WSBQPABSSA-N finasteride Chemical compound N([C@@H]1CC2)C(=O)C=C[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H](C(=O)NC(C)(C)C)[C@@]2(C)CC1 DBEPLOCGEIEOCV-WSBQPABSSA-N 0.000 description 1
- HVQAJTFOCKOKIN-UHFFFAOYSA-N flavonol Natural products O1C2=CC=CC=C2C(=O)C(O)=C1C1=CC=CC=C1 HVQAJTFOCKOKIN-UHFFFAOYSA-N 0.000 description 1
- 235000011957 flavonols Nutrition 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- KZJWDPNRJALLNS-STIDJNKJSA-N gamma-sitosterol Natural products CC[C@@H](CC[C@@H](C)[C@@H]1CC[C@H]2[C@H]3CC=C4C[C@@H](O)CC[C@]4(C)[C@H]3CC[C@]12C)C(C)C KZJWDPNRJALLNS-STIDJNKJSA-N 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000002290 gas chromatography-mass spectrometry Methods 0.000 description 1
- 239000003349 gelling agent Substances 0.000 description 1
- 235000008397 ginger Nutrition 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 208000037824 growth disorder Diseases 0.000 description 1
- 230000003648 hair appearance Effects 0.000 description 1
- 230000031774 hair cycle Effects 0.000 description 1
- 210000000642 hair follicle dermal papilla cell Anatomy 0.000 description 1
- 239000001307 helium Substances 0.000 description 1
- 229910052734 helium Inorganic materials 0.000 description 1
- SWQJXJOGLNCZEY-UHFFFAOYSA-N helium atom Chemical compound [He] SWQJXJOGLNCZEY-UHFFFAOYSA-N 0.000 description 1
- 239000003906 humectant Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000002401 inhibitory effect Effects 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 235000008777 kaempferol Nutrition 0.000 description 1
- MWDZOUNAPSSOEL-UHFFFAOYSA-N kaempferol Natural products OC1=C(C(=O)c2cc(O)cc(O)c2O1)c3ccc(O)cc3 MWDZOUNAPSSOEL-UHFFFAOYSA-N 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 239000002075 main ingredient Substances 0.000 description 1
- 238000004949 mass spectrometry Methods 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 230000004630 mental health Effects 0.000 description 1
- UXOUKMQIEVGVLY-UHFFFAOYSA-N morin Natural products OC1=CC(O)=CC(C2=C(C(=O)C3=C(O)C=C(O)C=C3O2)O)=C1 UXOUKMQIEVGVLY-UHFFFAOYSA-N 0.000 description 1
- 230000037257 muscle growth Effects 0.000 description 1
- 229930014626 natural product Natural products 0.000 description 1
- 230000001069 nematicidal effect Effects 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 239000003960 organic solvent Substances 0.000 description 1
- 230000035515 penetration Effects 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 230000000737 periodic effect Effects 0.000 description 1
- 229960005323 phenoxyethanol Drugs 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000010298 pulverizing process Methods 0.000 description 1
- 235000019633 pungent taste Nutrition 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000010992 reflux Methods 0.000 description 1
- 230000008929 regeneration Effects 0.000 description 1
- 238000011069 regeneration method Methods 0.000 description 1
- 230000026267 regulation of growth Effects 0.000 description 1
- 230000000284 resting effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 230000028327 secretion Effects 0.000 description 1
- 231100000872 sexual dysfunction Toxicity 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 1
- 239000004299 sodium benzoate Substances 0.000 description 1
- 235000010234 sodium benzoate Nutrition 0.000 description 1
- 229940057950 sodium laureth sulfate Drugs 0.000 description 1
- SXHLENDCVBIJFO-UHFFFAOYSA-M sodium;2-[2-(2-dodecoxyethoxy)ethoxy]ethyl sulfate Chemical compound [Na+].CCCCCCCCCCCCOCCOCCOCCOS([O-])(=O)=O SXHLENDCVBIJFO-UHFFFAOYSA-M 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000003381 stabilizer Substances 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 238000000194 supercritical-fluid extraction Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 229940033123 tannic acid Drugs 0.000 description 1
- 235000015523 tannic acid Nutrition 0.000 description 1
- 229920002258 tannic acid Polymers 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 229940124597 therapeutic agent Drugs 0.000 description 1
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- 231100000331 toxic Toxicity 0.000 description 1
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- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 238000010792 warming Methods 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
- 230000029663 wound healing Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/906—Zingiberaceae (Ginger family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/92—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof
- A61K8/922—Oils, fats or waxes; Derivatives thereof, e.g. hydrogenation products thereof of vegetable origin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P17/00—Drugs for dermatological disorders
- A61P17/14—Drugs for dermatological disorders for baldness or alopecia
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q7/00—Preparations for affecting hair growth
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- Health & Medical Sciences (AREA)
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- General Health & Medical Sciences (AREA)
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- Chemical & Material Sciences (AREA)
- Dermatology (AREA)
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- Engineering & Computer Science (AREA)
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- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The application provides a hair growth and hair loss prevention application of kaempferia galanga essential oil. Use of kaempferia galanga essential oil in the preparation of a composition for dermal application. The skin application composition is a hair growth and hair loss prevention medicine. The skin application composition is for promoting hair growth. The promoting hair growth includes promoting proliferation of human dermal papilla cells, and/or regulating levels of hair growth-related cell growth factors, and/or improving hair growth cycle. The preparation method of the kaempferia galanga essential oil comprises a steam distillation method or a supercritical carbon dioxide extraction method. The total content of cinnamate, olefin compounds, borneol and sitosterol in the rhizoma kaempferiae essential oil accounts for more than 90% of the total mass of the rhizoma kaempferiae essential oil. The skin application composition is applied to a pharmaceutical preparation or a daily chemical product. The kaempferia galanga essential oil is added into the pharmaceutical preparation or the daily chemical product in a mass percentage of 0.1% -20%.
Description
Technical Field
The application relates to the application field of plant essential oil, in particular to the application of kaempferia galanga essential oil in hair growth and hair loss prevention, and further application in the field of pharmaceutical preparations or daily chemicals.
Background
Alopecia is a common skin disease, clinically categorized into physiological alopecia and pathological alopecia, wherein physiological alopecia refers to the condition that healthy hair falls naturally from rest, also called rest-period alopecia, generally without special treatment. Pathologic alopecia refers to a phenomenon in which hair is abnormally or excessively lost, also called non-physiologic alopecia, in which androgenic alopecia (also called seborrheic alopecia) is the most common type of alopecia. It may not be a life threatening disease, but has a great impact on one's mental health and overall quality of life. More and more men and women suffer from hair loss or thinning due to environmental pollution, side effects of drugs, social environmental stress, and other factors. The research data of the alopecia Wei Jian Committee show that the number of men and women is 1.64 hundred million people and 0.87 hundred million people in the alopecia crowd in 2020, the proportion of male alopecia is obviously higher than that of women, and the female alopecia is in younger development trend. The cosmetic and pharmaceutical market for hair regrowth and hair loss prevention has grown significantly for decades. The currently clinically accepted therapeutic agents are minoxidil and the 5α -reductase inhibitor finasteride, but these agents all have various degrees of adverse side effects: such as sexual dysfunction, muscle growth disorders, hirsutism, local irritation and erythema, and the like. Therefore, other effective treatment schemes are urgently needed, and natural products are also an effective rich source for developing novel anti-hair loss agents.
The traditional Chinese medicine has long history of preventing and treating alopecia and has important significance in traditional medicine. The traditional Chinese medicine for preventing and treating alopecia is mainly deficiency tonifying, heat clearing, blood circulation promoting and blood stasis removing, wherein representative deficiency tonifying medicines such as polygonum multiflorum and ginseng; heat clearing type medicine kuh-seng and baikal skullcap root; safflower, etc. for activating blood and dissolving stasis. The periodic circulation of hair growth and shedding is regulated mainly by regulating the levels of some hormones, growth factors, cytokines and the like.
Rhizoma Kaempferiae, rhizoma Kaempferiae (Kaempferia galanga Linn) of Kaempferia of Zingiberaceae, is distributed in Guangdong, guangxi, yunnan, taiwan, etc. According to the records of Chinese pharmacopoeia (2020 edition), rhizoma kaempferiae has pungent taste, warm nature, spleen and stomach meridian, and has effects of activating qi-flowing, warming spleen and stomach, resolving food stagnation, and relieving pain, and also has unique fragrance, can be used as flavoring essence, and is one of medicinal materials with homology of medicine and food. In addition, kaempferia galanga is also commonly used for external use such as beautifying and cleaning teeth in ancient prescription, and modern domestic and foreign researches show that kaempferia galanga has activities of promoting wound healing, inhibiting pigmentation, preventing sunburn and the like in the aspect of skin pharmacology, can relax blood vessels, possibly plays a certain role in promoting hair growth, and is a medicinal material with great application potential.
The activity research of kaempferia galanga by the articles and patents published at home and abroad at present mainly focuses on: volatile components in rhizoma Kaempferiae have antibacterial, antiinflammatory, antioxidant, anticancer, and nematicidal effects, and flavonol compounds such as kaempferol and kaempferide have antioxidant, antitumor, antiinfectious, and blood lipid reducing effects. At present, in the existing patent 'a composition for removing dandruff and preventing alopecia, a preparation method and application thereof' (patent number CN 116035988A), kaempferia flavonoid component is applied to the aspects of removing dandruff and preventing alopecia; the patent of hair-blacking and hair-care essential oil and a preparation method thereof (patent number CN 110037946A) mentions that tannic acid component of kaempferia galanga essential oil can protect hair. However, in the prior researches, kaempferia galanga is not added as a main component of a hair care anti-hair loss product, the content is low, the specific components are unknown, the chemical components or the structure types of the chemical components are directly related to the activity of the chemical components, and flavonoids or tannins compounds play roles in the prescription; and the mechanism of how kaempferia galanga plays a role in hair growth is not emphasized or specifically described.
Disclosure of Invention
The embodiment of the application provides a hair growth and hair loss prevention application of kaempferia galangal essential oil, so as to solve the problems in the related art, and the technical scheme is as follows:
the embodiment of the application provides application of kaempferia galanga essential oil in preparing a skin application composition.
In one embodiment, the skin application composition is a hair growth, anti-hair loss drug.
In one embodiment, the skin application composition is for promoting hair growth.
In one embodiment, the promoting hair growth comprises promoting proliferation of human dermal papilla cells, and/or modulating levels of hair growth-related cell growth factors, and/or improving hair growth cycle.
In one embodiment, the preparation method of the kaempferia galanga essential oil comprises a steam distillation method or a supercritical carbon dioxide extraction method.
In one embodiment, the total content of cinnamate, olefin compound, borneol and sitosterol in the kaempferia galanga essential oil accounts for more than 90% of the total mass of the kaempferia galanga essential oil.
In one embodiment, the skin application composition is applied in a pharmaceutical formulation or a daily chemical product.
In one embodiment, the pharmaceutical formulation includes patches, tinctures, creams, cream formulations, ointments, gels, and sprays.
In one embodiment, the daily chemical product comprises a shampoo, a conditioner, an anti-hair-loss lotion, a hair-care essential oil, a hair-wash-hair-care essence, a hair-care oil, a hair-care lotion, a scalp-hair-show nourishment, a hair essence, a scalp cream, a hair-care gel, a hair spray, a hair-care film, and an eyebrow care solution.
In one embodiment, the kaempferia galanga essential oil is added into the pharmaceutical preparation or the daily chemical product in a mass percentage of 0.1% -20%.
The advantages or beneficial effects in the technical scheme at least comprise:
(1) The application provides the kaempferia galanga essential oil which is purely natural and free of organic solvent residues, and the essential oil is rich in raw material sources, simple and easy to obtain, and belongs to medicinal materials for both medicine and food.
(2) The application provides kaempferia galanga essential oil with hair growth and hair loss prevention effects, which comprises effective components of cinnamic acid esters, olefin compounds, borneol, sitosterol and the like; the essential oil and the active ingredients have small potential toxic and side effects, the essential oil is beneficial to skin penetration, and the essential oil has excellent effects on the aspect of in vivo and in vitro experiments, and can be used for preparing daily chemical products for promoting hair growth, fixing hair or preventing alopecia.
(3) The application provides a daily chemical product containing kaempferia galanga essential oil and used for promoting hair growth and preventing hair loss, which comprises but is not limited to shampoo products, anti-hair loss care solutions and the like, wherein the adding amount of the kaempferia galanga essential oil is 0.1-20% of the total mass of the daily chemical product, and the daily chemical product can achieve good effects of maintaining scalp health, preventing hair loss, nourishing hair and the like.
(4) Unlike the prior art, the following are: the application defines the types and the contents of volatile components contained in the kaempferia galangal essential oil, wherein the kaempferia galangal essential oil contains the mixture of various effective volatile components such as cinnamate, olefin compounds, borneol, sitosterol and the like, and the total content of the components reaches more than 90% of the essential oil; the rhizoma kaempferiae essential oil does not contain flavonoid or tannic acid compounds, which is the place different from the prior patent (such as the anti-dandruff and anti-drop ginger composition, and a preparation method and application thereof, patent number CN 116035988A); the kaempferia galanga essential oil and the main components thereof are used for activity research and application in the aspect of hair growth and hair loss prevention for the first time, and the hair growth and hair loss prevention effects of the kaempferia galanga essential oil formed by the components on the cellular and animal level are verified.
The foregoing summary is for the purpose of the specification only and is not intended to be limiting in any way. In addition to the illustrative aspects, embodiments, and features described above, further aspects, embodiments, and features of the present application will become apparent by reference to the drawings and the following detailed description.
Drawings
In the drawings, the same reference numerals refer to the same or similar parts or elements throughout the several views unless otherwise specified. The figures are not necessarily drawn to scale. It is appreciated that these drawings depict only some embodiments according to the disclosure and are not therefore to be considered limiting of its scope.
FIG. 1 is a graph showing the results of comparison of the cell viability of HFDPCs with different concentrations of kaempferia galangal essential oil in example 1 of the effect of the present application.
FIG. 2 is a graph showing the results of controlling hair growth-related cytokines in HFDPCs by kaempferia galangal essential oil in example 2 of the effects of the present application.
FIG. 3 is a graph showing the second result of controlling hair growth-related cytokines in HFDPCs by kaempferia galangal essential oil in effect example 2 of the present application.
FIG. 4 is a graph showing the comparison of hair growth areas on day 14 of application of kaempferia galangal essential oil to a natural hair growth model of a mouse in effect example 3 of the present application.
FIG. 5 is a graph showing the comparison of the lengths of the new hair after 21 days of application of kaempferia galangal essential oil to a natural hair growth model of a mouse in effect example 3 of the present application.
FIG. 6 is a graph showing the comparison of hair growth areas on day 14 of application of kaempferia galangal essential oil to an androgenic alopecia mouse model in effect example 4 of the present application.
Fig. 7 is a graph showing the comparison of the lengths of the new hair after 21 days of application of kaempferia galangal essential oil to an androgenic alopecia mouse model in effect example 4 of the present application.
Detailed Description
Hereinafter, only certain exemplary embodiments are briefly described. As will be recognized by those of skill in the pertinent art, the described embodiments may be modified in various different ways without departing from the spirit or scope of the present application. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive.
The application provides the pure natural kaempferia galanga essential oil which has excellent hair growing and hair loss preventing effects. The kaempferia galanga essential oil can be used for preventing or treating alopecia in the early stage by promoting proliferation of human dermal papilla cells (HFDPCs), up-regulating secretion of growth factor VEGF (vascular endothelial growth factor ), down-regulating inhibition factor TGF-beta 1 (transforming growth factor-beta, transforming growth factor beta), improving hair growth cycle and the like, and can overcome the defect that chemical medicines for promoting hair growth and preventing alopecia are not natural and safe enough in the prior art.
The embodiment of the application provides application of kaempferia galanga essential oil in preparing a skin application composition.
In one embodiment, the skin application composition is a hair growth, anti-hair loss drug.
In one embodiment, the skin application composition is for promoting hair growth.
In one embodiment, the promoting hair growth comprises promoting proliferation of human dermal papilla cells, and/or modulating levels of hair growth-related cell growth factors, and/or improving hair growth cycle.
In one embodiment, the preparation method of the kaempferia galanga essential oil comprises a steam distillation method or a supercritical carbon dioxide extraction method.
In one embodiment, the total content of cinnamate, olefin compound, borneol and sitosterol in the kaempferia galanga essential oil accounts for more than 90% of the total mass of the kaempferia galanga essential oil. The hair-growing and hair-loss-preventing effects of the kaempferia galangal essential oil mainly are achieved through the synergistic effect of the components.
In one embodiment, the skin application composition is applied in a pharmaceutical formulation or a daily chemical product.
In one embodiment, the pharmaceutical formulation includes patches, tinctures, creams, cream formulations, ointments, gels, and sprays.
In one embodiment, the daily chemical product comprises a shampoo, a conditioner, an anti-hair-loss lotion, a hair-care essential oil, a hair-wash-hair-care essence, a hair-care oil, a hair-care lotion, a scalp-hair-show nourishment, a hair essence, a scalp cream, a hair-care gel, a hair spray, a hair-care film, and an eyebrow care solution.
It should be understood that the daily chemical product may contain, in addition to the kaempferia galanga essential oil, general auxiliary components such as an emulsifier, a thickener, a surfactant, a humectant, alcohols, a water-soluble polymer, a gelling agent, a stabilizer, vitamins, inorganic salts, and a perfume. The auxiliary components are common raw materials in the prior art and are not repeated.
In one embodiment, the kaempferia galanga essential oil is added into the pharmaceutical preparation or the daily chemical product in a mass percentage of 0.1% -20%. By setting the kaempferia galanga essential oil in the proportion, the pharmaceutical preparation or daily chemical product can contain an effective amount of kaempferia galanga essential oil, so that the effects of hair growth and hair loss prevention are achieved.
Example 1 supercritical extraction method for preparing Kaempferia galanga essential oil
Pulverizing rhizoma Kaempferiae, taking a proper amount of rhizoma Kaempferiae, placing in a supercritical carbon dioxide extraction kettle, wherein the temperature of the extraction kettle is 40-60 ℃, the pressure of the extraction kettle is 15-35 MPa, the temperature of a separation kettle is 20-40 ℃, the pressure is 4-6 MPa, the temperature of condensate water is 5 ℃, the flow rate of carbon dioxide is 20-40 kg/h, and the extraction time is 1-3 h. The kaempferia galanga essential oil is obtained from the separation kettle, and the yield is 5-8%.
EXAMPLE 2 preparation of Kaempferia galanga essential oil by steam distillation
Adding fresh or dry rhizoma kaempferiae into a round-bottomed flask, adding 6-10 times of deionized water, and heating and refluxing with a volatile oil extraction device for 4-6 h. Standing after extraction, adding anhydrous sodium sulfate for water removal to obtain yellowish kaempferia galangal essential oil, wherein the yield is 0.5-1%.
For the above examples 1 or 2, kaempferia galangal essential oil can be obtained by extraction, optionally, the sample obtained in one step is diluted to a proper concentration by chromatographic grade ethyl acetate, and the active ingredient is subjected to qualitative and quantitative analysis by gas phase mass spectrometry (GC-MS):
chromatographic column DB-5MS capillary column (30 m x 0.25mm x 0.25 μm); the temperature of the ion source is 250 ℃; the mass range m/z is 30-500; the sample injection amount is 1 mu L; programming temperature: the initial temperature is 60 ℃, the temperature is kept for 5min, the temperature is increased to 180 ℃ at the speed of 15 ℃/min, and then the temperature is increased to 250 ℃ at the speed of 5 ℃/min, and the temperature is kept for 5min; the carrier gas is high-purity helium, split-flow sample injection is carried out, and the split ratio is 20:1, a step of; carrier gas flow rate 1mL/min; the standard chart library is an American NIST spectrum library, the relative content is determined by an area normalization method, and the quantitative result is shown in Table 1:
TABLE 1 Main ingredients and relative content in Kaempferia galanga essential oil
| Composition of the components | Molecular formula | Relative content (%) |
| P-methoxy cinnamic acid ethyl ester | C 12 H 14 O 3 | 49.34 |
| Pentadecane | C 15 H 32 | 18.79 |
| Trans-cinnamic acid ethyl ester | C 11 H 12 O 2 | 14.55 |
| Hexadecadiene | C 16 H 28 O | 4.34 |
| Cyperus-containing alkene | C 15 H 24 | 1.03 |
| N-heptadecane | C 17 H 36 | 0.99 |
| Juniper alkene | C 15 H 24 | 0.85 |
| 3-carene | C 10 H 16 | 0.51 |
| Gamma-sitosterol | C 29 H 50 O | 0.49 |
| Borneol | C 10 H 18 O | 0.40 |
Example 3 shampoo product containing Kaempferia galanga essential oil
Shampoo product containing kaempferia galanga essential oil has the components and contents shown in table 2:
table 2 contains the components and contents of the shampoo containing kaempferia galanga essential oil
| Component (A) | Mass percent (%) | Action |
| Rhizoma Kaempferiae essential oil | 2 | Hair growth, hair nourishing and hair loss preventing |
| Cocamidopropyl betaine | 3 | Surface active agent |
| Sodium laureth sulfate | 8 | Surface active agent |
| Citric acid | 0.3 | pH regulator and antidandruff agent |
| Phenoxyethanol | 0.1 | Preservative agent |
| Sodium benzoate | 0.1 | Preservative agent |
| Guar hydroxypropyl trimethyl ammonium chloride | 1 | Moisturizing softener |
| Pearling agent | 0.5 | |
| Deionized water | Complement 100 | Solvent(s) |
The preparation method comprises the following steps: and stirring and heating the deionized water and the surfactant according to the formula amount to 70 ℃, adding the kaempferia galangal essential oil after dissolution, stirring at constant temperature for 10min, adding the moisturizing softener, and stirring at constant temperature for 20min. Stopping heating and cooling after all the raw materials are completely dissolved, sequentially adding the preservative and the pearling agent when the temperature is reduced to below 45 ℃, regulating the pH value to 5.5-6.5 by using citric acid, stirring and cooling to room temperature.
Example 4 anti-drop Care solution containing Kaempferia galanga essential oil
The kaempferia galanga essential oil obtained in the example 1 is selected and dissolved in a solvent system to prepare anti-drop care solutions with the mass percentages of 0%, 0.1%, 1% and 10% respectively. Wherein the solvent system is 30% -40% absolute ethyl alcohol (dissolution), 50% propylene glycol (dissolution, corrosion prevention and moisture preservation) and 10% -20% deionized water.
Effect example 1 proliferation-promoting effect of kaempferia galanga essential oil on human dermal papilla cells (Hair Follicle Dermal Papilla Cells (HFDPCs))
Cell culture: hair papilla cells (HFDPCs) were cultured in Dulbecco's modified eagle's medium (DMEM, dulbecco Modified Eagle Medium) containing 10% (v/v) fetal bovine serum and 1% (v/v) antibiotics at 37℃with 5% CO 2 Culturing under the condition.
Sample preparation: the kaempferia galanga essential oil obtained in example 4 was dissolved in dimethyl sulfoxide (DMSO), and diluted with a medium to obtain kaempferia galanga essential oil of different concentrations (0.0002% -0.02%).
CCK-8 method detection: the cells were grown at 5X 10 3 Density of wells/Density of wells was inoculated in 96 well plates for 24h incubation, medium containing different concentrations of kaempferia galangal essential oil was added, and serum-free DMEM medium and 0.1% DMSO medium were used as blank and negative controls, respectively. After 24h of treatment, 10% CCK-8 solution was added to each well and incubated for a further 1h. The absorbance OD was measured at a detection wavelength of 450 nm. Cell viability (%) = (OD Sample of －OD Blank space )/(OD Negative control －OD Blank space ). The results are shown in FIG. 1. Under the co-incubation of kaempferia galanga essential oil with different concentrations, the cell activity is obviously improved as compared with a negative control group without essential oil, and the highest cell activity can reach 129.59%. It has been demonstrated that kaempferia galanga essential oil can promote proliferation of papilla cells, thereby promoting hair growth.
Effect example 2 modulation of hair growth-related cytokines in HFDPCs cells by kaempferia galangal essential oil
Cell culture: the same effects as in example 1.
Sample preparation: the same effect example 1, wherein the concentration of kaempferia galanga essential oil in the experimental group is 0.0002% -0.001%; in addition, minoxidil was prepared as a 0.0002% positive control group by the same method as the sample preparation.
The experimental method comprises the following steps: the regulation of growth factors VEGF (vascular endothelial growth factor ) and TGF-. Beta.1 (transforming growth factor-. Beta., transforming growth factor beta) in the culture supernatants obtained by treatment with ethyl p-methoxycinnamate was performed by ELISA kit (Xinbo, cat. No. EHC108.96, EHC107 b.96), and the results are shown in FIGS. 2 and 3.
The result shows that after the kaempferia galangal essential oil is given for co-incubation, the VEGF amount produced by human hair papilla cells is increased, and the VEGF plays a role in the growth of hair follicles and is a marked research index for promoting hair growth. On the other hand, minoxidil as a positive control significantly increased the amount of VEGF produced by human hair papilla cells compared to the blank control; in addition, the positive control and experimental groups with different concentrations of kaempferia galanga essential oil can obviously reduce the production of TGF-beta 1, the factor is generated by androgen being combined with androgen receptor after being converted by reductase, the apoptosis process of hair papilla cells is regulated, hair follicle atrophy is caused, and finally hair loss is caused.
Therefore, the result shows that after the kaempferia galangal essential oil is applied to scalp and the like, the production amount of cell factors related to hair growth can be increased, and the production of inhibition factors is resisted, so that the effects of hair growth and hair fixation can be achieved.
Effect example 3 hair growth effect of kaempferia galanga essential oil on mouse natural hair growth model application
Sample preparation: the dosage on the animal experiment level needs to be amplified on the cellular level. The kaempferia galanga essential oil prepared in the example 1 is dissolved in ethanol solution to prepare kaempferia galanga essential oil samples with the mass percentage of 5% and 1% for later use. And simultaneously, the internationally recognized alopecia treatment medicine minoxidil is dissolved in ethanol solution to be used as a positive control.
The experimental method comprises the following steps: the effect of kaempferia galanga essential oil on the growth of hair follicles of mice is tested by applying back dehaired skin. The C57BL/6 mice were divided into 4 groups, which were respectively an experimental group (1% Kaempferia galangal essential oil group and 5% Kaempferia galangal essential oil group), a positive control group and a blank control group, and were 4 groups of 3 mice each. After shaving the backs of the mice, 100 μl of kaempferia galangal essential oil samples (1% kaempferia galangal essential oil is applied to the test group 1, 5% kaempferia galangal essential oil is applied to the test group 2), 5% minoxidil is applied to the positive control group, and a blank solvent is applied to the blank control group for 14 consecutive days. The hair growth area (%) was calculated from the hair growth after 14 days of application. Hair growth area (%) =area of back hair newly grown/area of hair removed area × 100%.
The application was continued on day 21, and about 10 hairs were carefully pulled out from 6 different areas of the back of the mouse with forceps, and the length was measured with a vernier caliper and recorded as the longest value.
The hair growth area results are shown in fig. 4, and the hair regeneration area was calculated for each group of mice on day 14 of application. The color of the back skin of the C57BL/6 mice is closely related to the hair growth cycle, and the skin changes from pink to grey and black in the resting stage after depilation, indicating that the hair growth is again in the anagen stage. The results show that the back of the kaempferia galangal essential oil-treated mice grow new hair after being smeared for 14 days, the growth area is obviously larger than that of a blank control group, and the high-concentration kaempferia galangal essential oil-treated mice perform better than that of a positive group. The kaempferia galanga essential oil has a certain tendency of promoting the hair regeneration of mice.
The results of the length of the new hair are shown in fig. 5, and after 21 days of application, the hair regeneration was substantially completed in each group of mice. The positive control group and the experimental group with different concentrations of kaempferia galangal essential oil show better hair growth trend than the blank control group.
Effect example 4 anti-hair loss effect of kaempferia galanga essential oil on androgen alopecia mouse model
Sample preparation: the kaempferia galangal essential oil and the positive control minoxidil in example 1 were dissolved in ethanol solution respectively to prepare 5% concentration samples for use. Testosterone propionate was dissolved in corn oil for injection.
The experimental method comprises the following steps: the antagonism of kaempferia galanga essential oil on androgen inhibition hair growth is tested by applying the back dehaired skin. The C57BL/6 mice are divided into 4 groups, namely a blank control group, a model group, a positive control group and an experimental group, and 3 mice in each group are respectively used. Except for the blank group, testosterone propionate 5mg/kg was subcutaneously injected every day in the other 3 groups to induce alopecia, and after 30 minutes after injection, the blank control group and the model group were coated with a blank solvent, the positive control group were coated with 5% minoxidil, and the experimental group were coated with 5% kaempferia galangal essential oil, each 100 μl. The hair-regrowth area (%) was calculated by observation at day 14 of application, and the calculation formula was as in effect example 3.
The application was continued on day 21, and about 10 hairs were carefully pulled out from 6 different areas of the back of the mouse with forceps, and the length was measured with a vernier caliper and recorded as the longest value.
The calculation results of the hair regeneration area are shown in fig. 6, and fig. 6 shows that the ratio of the hair regeneration area on the back of each group of mice on the 14 th day of smearing shows that after 14 days of continuous androgen injection, the hair growth of the mice in the model group is blocked due to the action of hormone, and the hair regeneration area is obviously reduced compared with that of the blank control group, so that the modeling is successful; the effect of the experiment group kaempferia galanga essential oil after administration is similar to that of a positive control group, the experiment group kaempferia galanga essential oil can antagonize the hair growth inhibition caused by androgens, the effect of increasing the regeneration area is obvious compared with that of a model group, and the surface kaempferia galanga essential oil can antagonize the progress of androgenic alopecia to a certain extent and promote the hair growth of in-vivo mice.
The result of the length of the new hair is shown in fig. 7, the length of the new hair of the model group mice is shorter than that of the blank control group, and the positive control group and the kaempferia galangal essential oil experimental group can increase the length of the hair and have significance.
Effect example 5 Kaempferia galanga essential oil shampoo use feeling investigation
Volunteers aged 20-50 years were selected to be divided into two groups, and a use feeling test was performed on the shampoo containing kaempferia galangal essential oil prepared in example 3 or the comparative example (formulation as in example 3, kaempferia galangal essential oil was removed), and the results of a questionnaire about the use feeling are shown in table 3.
Table 3 evaluation of the feel of kaempferia galanga essential oil shampoo
| Comfortable feeling in use | Degree of cleanliness | Improvement of hair quality | Reducing hair loss | |
| Example 3 | ++ | ++ | +++ | +++ |
| Comparative example | ++ | ++ | ++ | - |
Note that: in the investigation result, — indicating that no findings, + indicates substantial satisfaction, ++ means that it is more satisfactory, +++ means non-positive is often satisfactory.
According to the effect test, the invention provides the kaempferia galangal essential oil-containing hair washing water, and the product has the effects of improving hair quality, strengthening hair roots and preventing hair loss compared with a comparative example.
Effect example 6 Kaempferia galanga essential oil anti-drop care solution use feeling investigation
Volunteers aged 20-50 years old were selected, and the anti-drop care solutions prepared in example 4 and containing different concentrations of kaempferia galangal essential oil were subjected to random blind testing for 3 human subjects for 1 month. The volunteers had the hair washed and then applied the care solution to the scalp once every two days, 5ml each time, and the results of the questionnaire about the effect of use are shown in table 4.
Table 4 evaluation of the effect of the kaempferia galanga essential oil anti-drop care solution
Note that: in the investigation result, — indicating that no findings, + indicates substantial satisfaction, ++ means that it is more satisfactory, +++ means non-positive is often satisfactory.
According to the effect test, the invention provides the anti-hair loss care solution containing the kaempferia galangal essential oil, and the product has the effects of improving scalp health, preventing hair loss and nourishing hair and has good use feeling.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the present application. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
The foregoing is merely specific embodiments of the present application, but the scope of the present application is not limited thereto, and any person skilled in the art can easily think of various changes or substitutions within the technical scope of the present application, and these should be covered in the scope of the present application. Therefore, the protection scope of the present application shall be subject to the protection scope of the claims.
Claims (10)
1. Use of kaempferia galanga essential oil in the preparation of a composition for dermal application.
2. The use according to claim 1, wherein the skin application composition is a hair growth, anti-hair loss medicament.
3. The use according to claim 2, wherein the skin application composition is for promoting hair growth.
4. The use according to claim 3, wherein said promoting hair growth comprises promoting proliferation of human dermal papilla cells, and/or regulating levels of hair growth-related cell growth factors, and/or improving hair growth cycle.
5. The use according to claim 1, wherein the preparation method of the kaempferia galangal essential oil comprises a steam distillation method or a supercritical carbon dioxide extraction method.
6. The use according to claim 1, wherein the total content of cinnamate, olefin compound, borneol and sitosterol in the kaempferia galanga essential oil is more than 90% of the total mass of the kaempferia galanga essential oil.
7. The use according to any one of claims 1 to 6, wherein the skin application composition is applied in a pharmaceutical formulation or in a daily chemical product.
8. The use according to claim 7, wherein the pharmaceutical formulation comprises a patch, a tincture, a cream formulation, an ointment, a gel and a spray.
9. The use according to claim 7, wherein the daily chemical product comprises a shampoo, a conditioner, an anti-hair loss lotion, a hair care essential oil, a hair wash-hair essence, a hair care oil, a hair care lotion, a scalp hair-care tonic, a hair essence, a scalp cream, a hair spray, a hair mask, and an eyebrow care solution.
10. The use according to claim 7, wherein the kaempferia galanga essential oil is added into the pharmaceutical preparation or the daily chemical product in a mass percentage of 0.1% -20%.
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