CN117481997B - Acne-removing repair composition containing recombinant collagen and application thereof - Google Patents
Acne-removing repair composition containing recombinant collagen and application thereof Download PDFInfo
- Publication number
- CN117481997B CN117481997B CN202311623336.9A CN202311623336A CN117481997B CN 117481997 B CN117481997 B CN 117481997B CN 202311623336 A CN202311623336 A CN 202311623336A CN 117481997 B CN117481997 B CN 117481997B
- Authority
- CN
- China
- Prior art keywords
- acne
- gel
- repairing
- recombinant collagen
- repair
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 206010000496 acne Diseases 0.000 title claims abstract description 69
- 208000002874 Acne Vulgaris Diseases 0.000 title claims abstract description 64
- 239000000203 mixture Substances 0.000 title claims abstract description 45
- 102000008186 Collagen Human genes 0.000 title claims abstract description 40
- 108010035532 Collagen Proteins 0.000 title claims abstract description 40
- 229920001436 collagen Polymers 0.000 title claims abstract description 40
- 230000008439 repair process Effects 0.000 title claims abstract description 29
- BDJRBEYXGGNYIS-UHFFFAOYSA-N nonanedioic acid Chemical compound OC(=O)CCCCCCCC(O)=O BDJRBEYXGGNYIS-UHFFFAOYSA-N 0.000 claims abstract description 52
- 230000000694 effects Effects 0.000 claims abstract description 42
- NVNLLIYOARQCIX-MSHCCFNRSA-N Nisin Chemical compound N1C(=O)[C@@H](CC(C)C)NC(=O)C(=C)NC(=O)[C@@H]([C@H](C)CC)NC(=O)[C@@H](NC(=O)C(=C/C)/NC(=O)[C@H](N)[C@H](C)CC)CSC[C@@H]1C(=O)N[C@@H]1C(=O)N2CCC[C@@H]2C(=O)NCC(=O)N[C@@H](C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(NCC(=O)N[C@H](C)C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCSC)C(=O)NCC(=O)N[C@H](CS[C@@H]2C)C(=O)N[C@H](CC(N)=O)C(=O)N[C@H](CCSC)C(=O)N[C@H](CCCCN)C(=O)N[C@@H]2C(N[C@H](C)C(=O)N[C@@H]3C(=O)N[C@@H](C(N[C@H](CC=4NC=NC=4)C(=O)N[C@H](CS[C@@H]3C)C(=O)N[C@H](CO)C(=O)N[C@H]([C@H](C)CC)C(=O)N[C@H](CC=3NC=NC=3)C(=O)N[C@H](C(C)C)C(=O)NC(=C)C(=O)N[C@H](CCCCN)C(O)=O)=O)CS[C@@H]2C)=O)=O)CS[C@@H]1C NVNLLIYOARQCIX-MSHCCFNRSA-N 0.000 claims abstract description 26
- 108010053775 Nisin Proteins 0.000 claims abstract description 26
- 239000004309 nisin Substances 0.000 claims abstract description 26
- 235000010297 nisin Nutrition 0.000 claims abstract description 26
- PWKSKIMOESPYIA-BYPYZUCNSA-N L-N-acetyl-Cysteine Chemical compound CC(=O)N[C@@H](CS)C(O)=O PWKSKIMOESPYIA-BYPYZUCNSA-N 0.000 claims abstract description 25
- 229960004308 acetylcysteine Drugs 0.000 claims abstract description 25
- 239000000284 extract Substances 0.000 claims description 23
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 21
- 238000002156 mixing Methods 0.000 claims description 20
- 238000002360 preparation method Methods 0.000 claims description 19
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 16
- 239000002537 cosmetic Substances 0.000 claims description 11
- 239000002994 raw material Substances 0.000 claims description 11
- 244000146553 Ceiba pentandra Species 0.000 claims description 10
- 235000003301 Ceiba pentandra Nutrition 0.000 claims description 10
- 239000002904 solvent Substances 0.000 claims description 10
- 235000011187 glycerol Nutrition 0.000 claims description 9
- GSEJCLTVZPLZKY-UHFFFAOYSA-N Triethanolamine Chemical compound OCCN(CCO)CCO GSEJCLTVZPLZKY-UHFFFAOYSA-N 0.000 claims description 7
- PUPZLCDOIYMWBV-UHFFFAOYSA-N (+/-)-1,3-Butanediol Chemical compound CC(O)CCO PUPZLCDOIYMWBV-UHFFFAOYSA-N 0.000 claims description 6
- 241000132446 Inula Species 0.000 claims description 6
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 6
- 241000196324 Embryophyta Species 0.000 claims description 5
- 239000003795 chemical substances by application Substances 0.000 claims description 5
- 238000000034 method Methods 0.000 claims description 5
- 230000003255 anti-acne Effects 0.000 claims description 4
- 239000003963 antioxidant agent Substances 0.000 claims description 4
- 230000003078 antioxidant effect Effects 0.000 claims description 4
- 229920005862 polyol Polymers 0.000 claims description 4
- 150000003077 polyols Chemical class 0.000 claims description 4
- 229920002125 Sokalan® Polymers 0.000 claims description 3
- 125000003118 aryl group Chemical group 0.000 claims description 3
- 239000002773 nucleotide Substances 0.000 claims description 3
- 125000003729 nucleotide group Chemical group 0.000 claims description 3
- 150000005846 sugar alcohols Polymers 0.000 claims description 3
- 239000004359 castor oil Substances 0.000 claims description 2
- 235000019438 castor oil Nutrition 0.000 claims description 2
- ZEMPKEQAKRGZGQ-XOQCFJPHSA-N glycerol triricinoleate Natural products CCCCCC[C@@H](O)CC=CCCCCCCCC(=O)OC[C@@H](COC(=O)CCCCCCCC=CC[C@@H](O)CCCCCC)OC(=O)CCCCCCCC=CC[C@H](O)CCCCCC ZEMPKEQAKRGZGQ-XOQCFJPHSA-N 0.000 claims description 2
- 239000000825 pharmaceutical preparation Substances 0.000 claims description 2
- 229940127557 pharmaceutical product Drugs 0.000 claims description 2
- NIXOWILDQLNWCW-UHFFFAOYSA-N Acrylic acid Chemical compound OC(=O)C=C NIXOWILDQLNWCW-UHFFFAOYSA-N 0.000 claims 2
- 229960001631 carbomer Drugs 0.000 claims 2
- 208000020154 Acnes Diseases 0.000 abstract description 14
- 241000186427 Cutibacterium acnes Species 0.000 abstract description 13
- 229940055019 propionibacterium acne Drugs 0.000 abstract description 13
- 230000002401 inhibitory effect Effects 0.000 abstract description 9
- 230000002195 synergetic effect Effects 0.000 abstract description 9
- 206010061218 Inflammation Diseases 0.000 abstract description 8
- 230000004054 inflammatory process Effects 0.000 abstract description 7
- 239000004615 ingredient Substances 0.000 abstract description 7
- 230000015572 biosynthetic process Effects 0.000 abstract description 6
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 230000003993 interaction Effects 0.000 abstract description 4
- 230000007246 mechanism Effects 0.000 abstract description 3
- 239000000047 product Substances 0.000 description 25
- 210000003491 skin Anatomy 0.000 description 23
- 239000000499 gel Substances 0.000 description 22
- 239000000243 solution Substances 0.000 description 20
- 230000000052 comparative effect Effects 0.000 description 18
- 238000012360 testing method Methods 0.000 description 9
- YPFDHNVEDLHUCE-UHFFFAOYSA-N propane-1,3-diol Chemical compound OCCCO YPFDHNVEDLHUCE-UHFFFAOYSA-N 0.000 description 8
- 230000001580 bacterial effect Effects 0.000 description 7
- 230000005764 inhibitory process Effects 0.000 description 7
- 210000004027 cell Anatomy 0.000 description 6
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 description 6
- 239000012488 sample solution Substances 0.000 description 6
- 239000004519 grease Substances 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- CDQSJQSWAWPGKG-UHFFFAOYSA-N butane-1,1-diol Chemical compound CCCC(O)O CDQSJQSWAWPGKG-UHFFFAOYSA-N 0.000 description 4
- 238000000855 fermentation Methods 0.000 description 4
- 230000004151 fermentation Effects 0.000 description 4
- 238000002835 absorbance Methods 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000003110 anti-inflammatory effect Effects 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 238000006243 chemical reaction Methods 0.000 description 3
- 238000012258 culturing Methods 0.000 description 3
- 238000011156 evaluation Methods 0.000 description 3
- 239000013604 expression vector Substances 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 239000003349 gelling agent Substances 0.000 description 3
- RAXXELZNTBOGNW-UHFFFAOYSA-N imidazole Natural products C1=CNC=N1 RAXXELZNTBOGNW-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 230000004048 modification Effects 0.000 description 3
- 238000012986 modification Methods 0.000 description 3
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 description 3
- 239000011148 porous material Substances 0.000 description 3
- 239000002244 precipitate Substances 0.000 description 3
- 102000004169 proteins and genes Human genes 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- 229960004889 salicylic acid Drugs 0.000 description 3
- 239000013598 vector Substances 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 2
- 239000004342 Benzoyl peroxide Substances 0.000 description 2
- OMPJBNCRMGITSC-UHFFFAOYSA-N Benzoylperoxide Chemical compound C=1C=CC=CC=1C(=O)OOC(=O)C1=CC=CC=C1 OMPJBNCRMGITSC-UHFFFAOYSA-N 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- 241000628997 Flos Species 0.000 description 2
- 241000219071 Malvaceae Species 0.000 description 2
- 229930195725 Mannitol Natural products 0.000 description 2
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 description 2
- 206010046865 Vaccinia virus infection Diseases 0.000 description 2
- SHGAZHPCJJPHSC-YCNIQYBTSA-N all-trans-retinoic acid Chemical compound OC(=O)\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C SHGAZHPCJJPHSC-YCNIQYBTSA-N 0.000 description 2
- 239000007864 aqueous solution Substances 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 235000019400 benzoyl peroxide Nutrition 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 230000001815 facial effect Effects 0.000 description 2
- 230000008014 freezing Effects 0.000 description 2
- 238000007710 freezing Methods 0.000 description 2
- 239000012737 fresh medium Substances 0.000 description 2
- LBQIJVLKGVZRIW-ZDUSSCGKSA-N glabridin Chemical compound C1([C@H]2CC3=CC=C4OC(C=CC4=C3OC2)(C)C)=CC=C(O)C=C1O LBQIJVLKGVZRIW-ZDUSSCGKSA-N 0.000 description 2
- 229940093767 glabridin Drugs 0.000 description 2
- PMPYOYXFIHXBJI-ZDUSSCGKSA-N glabridin Natural products C1([C@H]2CC=3C=CC4=C(C=3OC2)CCC(O4)(C)C)=CC=C(O)C=C1O PMPYOYXFIHXBJI-ZDUSSCGKSA-N 0.000 description 2
- LBQIJVLKGVZRIW-UHFFFAOYSA-N glabridine Natural products C1OC2=C3C=CC(C)(C)OC3=CC=C2CC1C1=CC=C(O)C=C1O LBQIJVLKGVZRIW-UHFFFAOYSA-N 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 230000002757 inflammatory effect Effects 0.000 description 2
- 230000007794 irritation Effects 0.000 description 2
- 229930027917 kanamycin Natural products 0.000 description 2
- SBUJHOSQTJFQJX-NOAMYHISSA-N kanamycin Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CN)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](N)[C@H](O)[C@@H](CO)O2)O)[C@H](N)C[C@@H]1N SBUJHOSQTJFQJX-NOAMYHISSA-N 0.000 description 2
- 229960000318 kanamycin Drugs 0.000 description 2
- 229930182823 kanamycin A Natural products 0.000 description 2
- 239000002502 liposome Substances 0.000 description 2
- 239000000594 mannitol Substances 0.000 description 2
- 235000010355 mannitol Nutrition 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 230000036619 pore blockages Effects 0.000 description 2
- 239000000843 powder Substances 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 229930002330 retinoic acid Natural products 0.000 description 2
- 210000001732 sebaceous gland Anatomy 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 229960001727 tretinoin Drugs 0.000 description 2
- 208000007089 vaccinia Diseases 0.000 description 2
- GOZMBJCYMQQACI-UHFFFAOYSA-N 6,7-dimethyl-3-[[methyl-[2-[methyl-[[1-[3-(trifluoromethyl)phenyl]indol-3-yl]methyl]amino]ethyl]amino]methyl]chromen-4-one;dihydrochloride Chemical compound Cl.Cl.C=1OC2=CC(C)=C(C)C=C2C(=O)C=1CN(C)CCN(C)CC(C1=CC=CC=C11)=CN1C1=CC=CC(C(F)(F)F)=C1 GOZMBJCYMQQACI-UHFFFAOYSA-N 0.000 description 1
- 238000012270 DNA recombination Methods 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 238000008157 ELISA kit Methods 0.000 description 1
- 244000252337 Epipremnum pinnatum Species 0.000 description 1
- 206010020649 Hyperkeratosis Diseases 0.000 description 1
- 108010002352 Interleukin-1 Proteins 0.000 description 1
- 208000001126 Keratosis Diseases 0.000 description 1
- 206010034972 Photosensitivity reaction Diseases 0.000 description 1
- 229940124158 Protease/peptidase inhibitor Drugs 0.000 description 1
- 206010040844 Skin exfoliation Diseases 0.000 description 1
- 206010040880 Skin irritation Diseases 0.000 description 1
- 206010070835 Skin sensitisation Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000000172 allergic effect Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 125000000129 anionic group Chemical group 0.000 description 1
- 230000002421 anti-septic effect Effects 0.000 description 1
- 208000010668 atopic eczema Diseases 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960003328 benzoyl peroxide Drugs 0.000 description 1
- 230000000903 blocking effect Effects 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 239000012539 chromatography resin Substances 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 230000003750 conditioning effect Effects 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 239000012228 culture supernatant Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000035618 desquamation Effects 0.000 description 1
- 238000000502 dialysis Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000003467 diminishing effect Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003480 eluent Substances 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 210000000245 forearm Anatomy 0.000 description 1
- 235000021588 free fatty acids Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 229960000789 guanidine hydrochloride Drugs 0.000 description 1
- PJJJBBJSCAKJQF-UHFFFAOYSA-N guanidinium chloride Chemical compound [Cl-].NC(N)=[NH2+] PJJJBBJSCAKJQF-UHFFFAOYSA-N 0.000 description 1
- 210000003780 hair follicle Anatomy 0.000 description 1
- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 1
- 230000003780 keratinization Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 229940126601 medicinal product Drugs 0.000 description 1
- 210000004400 mucous membrane Anatomy 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 229910052759 nickel Inorganic materials 0.000 description 1
- 230000000474 nursing effect Effects 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 239000000137 peptide hydrolase inhibitor Substances 0.000 description 1
- 230000036211 photosensitivity Effects 0.000 description 1
- 230000019612 pigmentation Effects 0.000 description 1
- 239000000419 plant extract Substances 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000037307 sensitive skin Effects 0.000 description 1
- 230000008313 sensitization Effects 0.000 description 1
- 230000001953 sensory effect Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 230000008591 skin barrier function Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 230000036556 skin irritation Effects 0.000 description 1
- 231100000475 skin irritation Toxicity 0.000 description 1
- 231100000370 skin sensitisation Toxicity 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- 230000000699 topical effect Effects 0.000 description 1
- 150000003626 triacylglycerols Chemical class 0.000 description 1
- 229940098386 usnea barbata extract Drugs 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
- 239000003643 water by type Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/04—Dispersions; Emulsions
- A61K8/042—Gels
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/36—Carboxylic acids; Salts or anhydrides thereof
- A61K8/362—Polycarboxylic acids
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/40—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing nitrogen
- A61K8/44—Aminocarboxylic acids or derivatives thereof, e.g. aminocarboxylic acids containing sulfur; Salts; Esters or N-acylated derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q17/00—Barrier preparations; Preparations brought into direct contact with the skin for affording protection against external influences, e.g. sunlight, X-rays or other harmful rays, corrosive materials, bacteria or insect stings
- A61Q17/005—Antimicrobial preparations
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- General Health & Medical Sciences (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Birds (AREA)
- Epidemiology (AREA)
- Dermatology (AREA)
- Emergency Medicine (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Dispersion Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Cosmetics (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
The invention relates to an acne-removing and repairing composition containing recombinant collagen and application thereof. The invention creatively combines the four components, wherein azelaic acid, nisin and acetylcysteine have potential interaction promotion effects in inhibiting propionibacterium acnes and resisting inflammation, so that the invention has synergistic effects in inhibiting and relieving the formation of acnes and repairing the acnes; the recombinant collagen can repair skin damaged by acnes and inflammations, can provide sufficient nutritional ingredients for the damaged skin to help the skin to repair better, and can further promote the effects of azelaic acid, nisin and acetylcysteine on acne removal, and the four ingredients play roles of acne removal and repair jointly from different angles of different mechanisms. Meanwhile, the composition can well give consideration to excellent acne removal and repair effects and excellent mildness.
Description
Technical Field
The invention belongs to the technical field of acne-removing efficacy products, and relates to an acne-removing repair composition containing recombinant collagen and application thereof.
Background
Acne, also known as whelk, comedo, pimple, is a common and frequent disease state of the skin. The root cause of the formation is a chronic skin disease occurring in the pilosebaceous glands, and the phenomenon of pore blocking is generated due to the vigorous secretion of grease. The cosmetic effect is affected by the abundant sebaceous glands such as head, face, neck and the like.
Propionibacterium acnes is considered to be the most predominant bacterium causing acnes because excessive grease is secreted in the skin, the sebaceous cells are excessively proliferated, the grease is secreted into sebaceous glands and spread in pores, after propionibacterium acnes contacts the grease, triglycerides in the grease are decomposed to form glycerin and fatty acids, the glycerin increases nutrition of bacteria, proliferation of bacteria is caused, and free fatty acids cause keratinization of skin pores, hyperkeratinization, and pore blockage is caused. Bacterial proliferation, pore blockage, and vaccinia formation.
The topical medicinal products for removing acne include retinoic acid, benzoyl peroxide, and antibiotics. However, retinoic acid increases the photosensitivity of the skin and reds the skin; benzoyl peroxide has certain irritation, and individual patients can have reactions such as redness, desquamation and the like; antibiotics can cause bacterial drug resistance, and the using course of the antibiotics cannot be too long.
The prior art also discloses a few types of cosmetics with acne removing effect, for example, CN107737033A discloses a composition for rapidly removing acnes and acne marks and a preparation method thereof, and the raw materials comprise the following components in parts by weight: 1.2 to 1.8 parts of salicylic acid, 2 to 2.77 parts of arginine, 0.5 to 2 parts of Usnea barbata extract, 1 to 10 parts of glabridin liposome, 0.03 to 0.1 part of O-cymene-5 alcohol, 1 to 12 parts of other acne-removing efficacy components and 75 to 90 parts of other base materials. The product is prepared by combining salicylic acid with arginine with specific content, compounding with scindapsus aureus extract and glabridin liposome with specific content, and adding O-cymene-5 alcohol as a mild and effective antiseptic bactericide and other acne-removing functional components. However, the raw material contains salicylic acid, so that skin and mucous membrane can be stimulated, skin barrier can be damaged, allergic symptoms can be caused, and the raw material is not suitable for people with sensitive skin.
At present, the acne-removing cosmetics on the market are various in variety, the production process and the formula of the cosmetics are basically different in size, and the cosmetics have the effects of protecting and beautifying the human skin to different degrees, but the effects are not ideal, and the cosmetics have no multiple effects of diminishing inflammation, repairing and nursing on facial acnes. And after the products are coated, the products generally lose efficacy in a short time, the functional components are difficult to act on the skin for a long time, the purpose of acne removal is achieved, and the efficacy is difficult to ensure.
Therefore, it is very significant to develop a product which is mild, safe and capable of removing acnes for a long time.
Disclosure of Invention
Aiming at the defects of the prior art, the invention aims to provide an acne-removing and repairing composition containing recombinant collagen and application thereof.
In order to achieve the aim of the invention, the invention adopts the following technical scheme:
In a first aspect, the present invention provides an anti-acne repair composition comprising recombinant collagen, the anti-acne repair composition comprising azelaic acid, nisin, acetylcysteine and recombinant collagen.
Azelaic acid has antibacterial, antiinflammatory, hair follicle keratosis regulating, oil secretion inhibiting, pigmentation reducing, and cell proliferation inhibiting effects; nisin can interact with anionic lipids on bacterial cell membranes to cause perturbation of the cell membranes to inhibit bacteria; acetylcysteine can be used as skin conditioner, antioxidant or protease inhibitor in cosmetics and skin care products; the recombinant collagen is collagen prepared by using a DNA recombination technology, can supplement amino acid required by skin, repair damaged skin and provide necessary nutritional ingredients for the skin.
The invention creatively combines four components of azelaic acid, nisin, acetylcysteine and recombinant collagen, wherein the azelaic acid, nisin and acetylcysteine have potential interaction promotion effects in inhibiting propionibacterium acnes and resisting inflammation, thereby having synergistic effects in inhibiting and relieving the formation of acnes and repairing the acnes; the recombinant collagen can repair skin damaged by acnes and inflammations, can provide sufficient nutritional ingredients for the damaged skin to help the skin to repair better, and can further promote the effects of azelaic acid, nisin and acetylcysteine on acne removal, and the four ingredients play roles of acne removal and repair jointly from different angles of different mechanisms. Meanwhile, the composition can well give consideration to excellent acne removal and repair effects and excellent mildness.
Preferably, the acne-removing and repairing composition comprises, by mass, 4-10 parts of azelaic acid, 0.1-1 part of nisin, 0.5-5 parts of acetylcysteine and 0.01-1 part of recombinant collagen.
Based on the potential interaction among the components, when the four components are combined in a specific mass ratio relationship, the composition has better effects on removing acnes and repairing skin.
The azelaic acid may be 4 parts, 4.5 parts, 5 parts, 5.5 parts, 6 parts, 6.5 parts, 7 parts, 7.5 parts, 8 parts, 8.5 parts, 9 parts, 9.5 parts, 10 parts, etc.; the nisin may be 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.6 part, 0.7 part, 0.8 part, 0.9 part, 1 part, etc.; the acetylcysteine may be 0.5 part, 1 part, 1.5 parts, 2 parts, 2.5 parts, 3 parts, 3.5 parts, 4 parts, 4.5 parts, 5 parts, etc.; the recombinant collagen may be 0.01 part, 0.03 part, 0.05 part, 0.08 part, 0.1 part, 0.2 part, 0.3 part, 0.4 part, 0.5 part, 0.7 part, 0.8 part, 1 part, etc. Other specific point values in the numerical ranges are selectable, and will not be described in detail herein.
Preferably, the nucleotide sequence encoding the recombinant collagen is the sequence shown in SEQ ID NO. 1.
Compared with other sources of recombinant collagen, the recombinant collagen from the specific source has better effects on bacteriostasis, anti-inflammation, acne removal and skin repair.
SEQ ID NO:1：
GGGCCTCAAGGTATTGCTGGACAGCGTGGTGTGGTCGGCCTGCCTGGTCAGAGAGGAGAGAGAGGCTTCCCTGGTCTTCCTGGCCCCTCTGGTGAACCTGGCAAACAAGGTCCCTCTGGAGCAAGTGGTGAACGTGGTCCCCCTGGTCCCATGGGCCCCCCTGGATTGGCTGGACCCCCTGGTGAATCTGGACGTGAGGGGGCTCCTGGTGCCGAAGGTTCCCCTGGACGAGACGGTTCTCCTGGCGCCAAGGGTGACCGTGGTGAGACCGGCCCCGCTGGACCCCCTGGTGCTCCTGGTGCTCCTGGTGCCCCTGGCCCCGTTGGCCCTGCTGGCAAGAGTGGTGATCGTGGTGAGACTGGTCCTGCTGGTCCCGCCGGAGAACGAGGTGGCCCTGGAGGACCTGGCCCTCAGGGTCCTCCTGGAAAGAATGGTGAAACTGGACCTCAGGGACCCCCAGGGCCTACTGGGCCTGGTGGTGACAAAGGAGACACAGGACCCCCTGGTCCACAAGGATTACAAGGCTTGCCTGGTACAGGTGGTCCTCCAGGAGAAAATGGAAAACCTGGGGAACCAGGTCCAAAGGGTGATGCCGGTGCACCTGGAGCTCCAGGAGGCAAGGGTGATGCTGGTGCCCCTGGTGAACGTGGACCTCCTGGATTGGCAGGGGCCCCAGGACTTAGAGGTGGAGCTGGTCCCCCTGGTCCCGAAGGAGGAAAGGGTGCTGCTGGTCCTCCTGGGCCACCTGGTGCTGCTGGTACTCCTGGTCTGCAAGGAATGCCTGGACCTGGTCCTTGCTGTGGTGGTTAA.
In a second aspect, the invention provides an application of the acne-removing and repairing composition containing the recombinant collagen according to the first aspect in preparing an acne-removing and repairing effect product.
Preferably, the additive amount of the acne removing and repairing composition in the acne removing and repairing effect product is 1-20%, for example 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 20%, etc., and other specific point values in the numerical range can be selected, which will not be described in detail herein.
Preferably, the product comprises a cosmetic or pharmaceutical product.
In a third aspect, the invention provides a gel with acne-removing and repairing effects, and the gel comprises the following preparation raw materials in percentage by mass: 1-20% of the acne-removing repair composition in the first aspect, 0.5-5% of a gel, 0.2-2% of a solubilizer, 5-20% of a polyalcohol, 0.1-1% of triethanolamine and 50-90% of water.
The mass percentage of the acne-removing and repairing composition can be selected to be 1%, 2%, 3%, 4%, 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 20% and the like; the mass percentage of the gel can be selected to be 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2%, 3%, 4%, 5% and the like; the mass percentage of the solubilizer can be selected to be 0.2%, 0.3%, 0.5%, 0.6%, 0.8%, 1%, 1.2%, 1.4%, 1.6%, 1.8%, 2% and the like; the mass percent of the polyol can be selected to be 5%, 6%, 7%, 8%, 9%, 10%, 12%, 14%, 15%, 20%, etc.; the weight percentage of the triethanolamine can be selected to be 0.1%, 0.2%, 0.3%, 0.4%, 0.5%, 0.7%, 0.8%, 0.9%, 1% and the like; the mass percentage of the water can be selected to be 50%, 60%, 65%, 70%, 75%, 80%, 85%, 90% and the like; other specific point values within the above numerical ranges are all selectable, and will not be described in detail herein.
Preferably, the gelling agent comprises carbomers.
Preferably, the solubilising agent comprises PPG-26-butanol polyether-26 and/or PEG-40 hydrogenated castor oil.
Preferably, the polyol comprises any one or a combination of at least two of propylene glycol, butylene glycol or glycerin.
Preferably, the gel preparation comprises the following raw materials in percentage by mass: 1-10% of plant source conditioner, 0.05-0.5% of antioxidant or 0.1-0.5% of aromatic agent or the combination of at least two.
The mass percentage of the plant source conditioning agent can be selected to be 1%, 2%, 3%, 4%, 5%, 6%, 8%, 10% and the like; the antioxidant may be selected from 0.05%, 0.08%, 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5%, etc.; the mass percentage of the aromatic agent can be selected to be 0.1%, 0.15%, 0.2%, 0.25%, 0.3%, 0.4%, 0.5% and the like; other specific point values within the above numerical ranges are all selectable, and will not be described in detail herein.
Preferably, the plant source conditioner comprises an extract of a square flower and/or an extract of kapok.
The invention also creatively discovers that when the extract of the inula flowers and/or the extract of the kapok flowers, especially the combination of the extract of the inula flowers and the extract of the kapok flowers, are further added into the product, the antibacterial, anti-inflammatory and acne-removing effects of the product can be further improved. Wherein the extract of flos Lupuli and the extract of flos kapok have synergistic effect in exerting the above effects.
In a fourth aspect, the present invention provides a method for preparing the gel with acne-removing and repairing effects according to the third aspect, the method comprising the following steps:
Mixing the gel with part of water at 25-45 ℃ to obtain a solution A; mixing the acne-removing repair composition, the solubilizer, the polyol and the residual water at 30-50 ℃ to obtain a solution B; mixing the solution A and the solution B at 20-40 ℃, and adding triethanolamine and the rest preparation raw materials to obtain the gel with acne removing and repairing effects.
The specific point value of 25-45deg.C can be 25deg.C, 28deg.C, 30deg.C, 32deg.C, 35deg.C, 38deg.C, 40deg.C, 42deg.C, 45deg.C, etc.; the specific point value of 30-50deg.C can be 30 deg.C, 33 deg.C, 35 deg.C, 38 deg.C, 40 deg.C, 42 deg.C, 45 deg.C, 48 deg.C, 50 deg.C, etc.; the specific values of 20-40deg.C can be 20deg.C, 22deg.C, 25deg.C, 28deg.C, 30deg.C, 32deg.C, 35deg.C, 38deg.C, 40deg.C, etc.; other specific point values within the above numerical ranges are all selectable, and will not be described in detail herein.
Compared with the prior art, the invention has the following beneficial effects:
The invention creatively combines four components of azelaic acid, nisin, acetylcysteine and recombinant collagen, wherein the azelaic acid, nisin and acetylcysteine have potential interaction promotion effects in inhibiting propionibacterium acnes and resisting inflammation, thereby having synergistic effects in inhibiting and relieving the formation of acnes and repairing the acnes; the recombinant collagen can repair skin damaged by acnes and inflammations, can provide sufficient nutritional ingredients for the damaged skin to help the skin to repair better, and can further promote the effects of azelaic acid, nisin and acetylcysteine on acne removal, and the four ingredients play roles of acne removal and repair jointly from different angles of different mechanisms. Meanwhile, the composition can well give consideration to excellent acne removal and repair effects and excellent mildness.
Detailed Description
In order to further describe the technical means adopted by the present invention and the effects thereof, the following describes the technical scheme of the present invention in combination with the preferred embodiments of the present invention, but the present invention is not limited to the scope of the embodiments.
The mass parts of each component in the composition product referred to in the following are calculated according to the actual content of the effective components in the raw materials sold in the market.
The extract of Fanghua involved in the following is derived from the product of Waters biotechnology Co., ltd; kapok extract is derived from the product of the biological sciences company, vortex, lan; nisin is derived from products of guangzhou Tianjia biotechnology limited.
The recombinant collagen referred to in the following was prepared using the product prepared in preparation examples in which pET-28a (+) vector was purchased from Michelin; BL21 (DE 3) chemocompetent cells were commercially available from Merck under the designation CMC0014-4X40 UL.
Preparation example
The preparation example provides recombinant collagen freeze-dried powder, and the preparation method is carried out by referring to the method disclosed in the preparation example 1 in ZL 202310123476.3, and specifically comprises the following steps:
(1) Constructing a recombinant plasmid: according to the nucleotide sequence shown in SEQ ID NO. 1, selecting a pET-28a (+) vector suitable for an expression vector escherichia coli host, and sending the pET-28a (+) vector to a sequencing company for synthesis to prepare a recombinant collagen expression vector pET-28a-1.
(2) Recombinant fermentation strain construction: transferring the pET-28a-1 expression vector into competent cells of escherichia coli (BL 21 (DE 3)) by using a chemical conversion method, screening positive clones in plate colonies after conversion by agarose gel electrophoresis, transferring the screened positive clones to 2mL of LB culture medium, culturing for 12 hours at 37 ℃ and 200rpm, diluting the cultured bacterial liquid by 1 time with glycerol with the volume fraction of 50%, packaging into a freezing tube, and freezing at the temperature of minus 80 ℃ to obtain the recombinant collagen fermentation strain.
(3) Fermenting and culturing recombinant fermentation strains: 10 mu L of recombinant collagen fermentation broth is coated on a solid LB plate, cultured for 12 hours in a 37 ℃ incubator, selected and inoculated in 50mL of LB liquid medium containing 50 mu g/mL kanamycin content, placed in a shaking table for culture at 200rpm for 14 hours at 37 ℃, then inoculated in 200mL of LB liquid medium containing 50 mu g/mL kanamycin content at a ratio of 1:100, cultured until the bacterial broth OD=0.6 at 37 ℃, added with IPTG to achieve the final concentration of 1mM, continuously cultured for 4 hours, then centrifuged at 4000rpm for 15 minutes, and the bacterial cells are collected.
(4) Purifying recombinant collagen: the bacterial cells are subjected to ultrasonic disruption and centrifugation, the precipitate is washed by using a TritonX-100 washing solution, the supernatant is removed by centrifugation again, 6M guanidine hydrochloride is added into the precipitate, the pH is regulated to 8, after the precipitate is fully dissolved, the solution is subjected to nickel affinity chromatography resin, 20mM, 50mM, 100mM, 200mM and 500mM concentration gradient imidazole solution is used for eluting in sequence, eluent is concentrated by using a protein concentration column, the concentrated solution is collected and dialyzed overnight (dialysis bag, RC film, 14KD and flat width of 44mM (manufacturer F600112)), then the protein concentration is detected by using a BCA kit, and the solution is uniformly mixed with the mannitol aqueous solution according to the proportion of 0.5mL of mannitol aqueous solution with the volume concentration of 5 percent per 5mg of protein, and then the solution is pre-frozen at-40 ℃ for 10min by using a freeze dryer, and finally freeze-dried at-35 ℃ to obtain the recombinant collagen freeze-dried powder.
Example 1
The embodiment provides an acne-removing and repairing composition which comprises the following components in parts by mass: 5 parts of azelaic acid, 1 part of nisin, 3 parts of acetylcysteine and 0.08 part of recombinant collagen. The components are physically and evenly mixed according to the mass ratio.
Example 2
The embodiment provides an acne-removing and repairing composition which comprises the following components in parts by mass: 4 parts of azelaic acid, 0.5 part of nisin, 5 parts of acetylcysteine and 0.05 part of recombinant collagen. The components are physically and evenly mixed according to the mass ratio.
Example 3
The embodiment provides an acne-removing and repairing composition which comprises the following components in parts by mass: azelaic acid 7 parts, nisin 0.2 parts, acetylcysteine 2 parts and recombinant collagen 0.03 parts. The components are physically and evenly mixed according to the mass ratio.
Comparative example 1
This comparative example provides a composition which differs from example 1 only in that azelaic acid is absent, its reduced mass is apportioned to the mass of nisin and acetylcysteine, the other components and amounts remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Comparative example 2
This comparative example provides a composition which differs from example 1 only in that nisin is not present, and its reduced mass is apportioned to the mass of azelaic acid and acetylcysteine, the other components and amounts remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Comparative example 3
This comparative example provides a composition which differs from example 1 only in that it does not contain acetylcysteine, and its reduced mass is apportioned to the mass of azelaic acid and nisin, the other components and amounts remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Comparative example 4
This comparative example provides a composition which differs from example 1 only in that it does not contain recombinant collagen, its reduced mass being assigned to the mass of azelaic acid, the other components and amounts remaining unchanged. The components are physically and evenly mixed according to the mass ratio.
Application example 1
The application example provides a gel, which comprises the following components:
The preparation method comprises the following steps:
(1) Mixing the gel with part of water at 30 ℃ to obtain a solution A;
(2) Mixing azelaic acid with 1, 3-propylene glycol and butanediol at 40deg.C, and mixing with nisin, acetylcysteine, recombinant collagen, solubilizer, glycerol and residual water at 40deg.C to obtain solution B;
(3) Mixing the solution A and the solution B at 30 ℃, and adding triethanolamine to obtain the gel with acne removing and repairing effects.
Application examples 2 to 3
The application example provides two gels, and the formula of the gel is different from that of application example 1 only in that the acne-removing repair composition of example 1 is replaced by the acne-removing repair composition prepared in the same amount of examples 2-3, and other components and contents are kept unchanged. The preparation method is reference application example 1.
Comparative application examples 1 to 4
Comparative examples 1 to 4 provided four gels, the formulation of which was different from that of example 1 only in that the acne-removing restoration composition prepared in example 1 was replaced with the same amount of the acne-removing restoration composition prepared in comparative examples 1 to 4, and the other components and contents were kept unchanged. The preparation method is reference application example 1.
Application example 4
The application example provides a gel, which comprises the following components:
The preparation method comprises the following steps:
(1) Mixing the gel with part of water at 35 ℃ to obtain a solution A;
(2) Mixing azelaic acid with 1, 3-propylene glycol and butanediol at 30deg.C, and mixing with nisin, acetylcysteine, recombinant collagen, solubilizer, glycerol and residual water at 30deg.C to obtain solution B;
(3) Mixing the solution A and the solution B at 30 ℃, and adding triethanolamine and a conditioner to obtain the gel with acne removing and repairing effects.
Application example 5
The application example provides a gel, and the formula of the gel is different from that of application example 4 only in that the extract of the common bombax flower is lacking, the mass percentage of the extract of the common bombax flower is 8%, and other components and contents are kept unchanged.
Application example 6
The application example provides a gel, and the formula of the gel is different from that of application example 4 only in that kapok extract is absent, the mass percentage of plain flower extract is 8%, and other components and contents are kept unchanged.
Application example 7
The application example provides a gel, the formula of which is different from that of application example 1 only in that the acne-removing repairing composition is lack, the reduced quality is complemented by water, and the other components and the content are kept unchanged.
Test example 1
Safety evaluation:
placing the products prepared in application examples 1-7 and comparative application examples 1-4 in a patch tester, attaching the patch tester with the test object to the forearm flexor side of the tested person (20), and uniformly applying the patch to the skin by lightly pressing the palm for 24 hours; skin irritation and sensitization were observed as shown in Table 1 below 30min, 24h, 48h after removal of the subject plaque tester, respectively, and the observations were recorded.
TABLE 1
The results show that the products prepared in application examples 1-7 and comparative application examples 1-4 have a rating of 0, which indicates that the acne-removing repair composition according to the invention has no irritation to skin.
Test example 2
Propionibacterium acnes inhibition assay:
Preparing a sample solution: sample solutions 1 to 11 correspond to application examples 1 to 7 and comparative application examples 1 to 4, respectively, and their formulations differ from application examples 1 to 7 and comparative application examples 1 to 4 in that they do not contain solubilizers, gelling agents and glycerin, and their reduced mass is complemented by water. The preparation method comprises the following steps: mixing azelaic acid with 1, 3-propylene glycol and butanediol at 30deg.C, mixing with nisin, acetylcysteine, recombinant collagen and water at 30deg.C, optionally adding conditioner, and mixing.
Propionibacterium acnes was inoculated into 96-well plates at 1X 10 6/mL, 150. Mu.L of each well was added with 150. Mu.L of the sample solution 1-11, and the culture medium of the same volume was added as a blank, and incubated in a constant temperature incubator at 37℃for 48 hours under anaerobic conditions. The absorbance at 600nm was measured by an ultraviolet spectrophotometer, and the propionibacterium acnes inhibition (%) was calculated according to the following formula, and each group was measured in parallel 5 times, and the average was taken.
Propionibacterium acnes inhibition (%) = (blank absorbance-test absorbance)/blank absorbance x 100%
The results are shown in Table 2.
TABLE 2
| Group of | Propionibacterium acnes inhibition rate (%) |
| 1 | 76.9 |
| 2 | 71.5 |
| 3 | 70.8 |
| 4 | 85.2 |
| 5 | 80.3 |
| 6 | 82.1 |
| 7 | 7.6 |
| 8 | 58.8 |
| 9 | 62.5 |
| 10 | 67.2 |
| 11 | 79.4 |
As shown in table 2, the acne-removing and repairing composition has a remarkable propionibacterium acnes inhibition effect, azelaic acid, nisin and acetylcysteine have a synergistic effect on the propionibacterium acnes inhibition effect, and the extract of inula flowers and the extract of kapok flowers can have the effects and have the synergistic effect.
Test example 3
Propionibacterium acnes inhibition assay:
Preparing a sample solution: sample solutions 1 to 11 correspond to application examples 1 to 7 and comparative application examples 1 to 4, respectively, and their formulations differ from application examples 1 to 7 and comparative application examples 1 to 4 in that they do not contain solubilizers, gelling agents and glycerin, and their reduced mass is complemented by water. The preparation method comprises the following steps: mixing azelaic acid with 1, 3-propylene glycol and butanediol at 30deg.C, mixing with nisin, acetylcysteine, recombinant collagen and water at 30deg.C, optionally adding conditioner, and mixing.
1X 10 6/mL Raw264.7 cells were inoculated into 24-well plates, 300. Mu.L per well, placed in a 37℃incubator for 24 hours, and the culture supernatant was discarded. 300 mu L of fresh medium is added into the blank group, 300 mu L of fresh medium dissolved with 1 mu g/mL of LPS is added into the LPS group, 300 mu L of sample solution 1-11 dissolved with 1 mu g/mL of LPS is respectively added into the test group, and the test groups are placed in a constant temperature incubator at 37 ℃ for culturing for 24 hours. The supernatant was centrifuged and the concentrations of the inflammatory factors TNF- α and IL-1 were measured according to the test procedure on ELISA kit and each group was assayed 5 times in parallel and the average was taken and the results are shown in Table 3.
TABLE 3 Table 3
As shown in table 3, the acne-removing and repairing composition has a remarkable anti-inflammatory effect, azelaic acid, nisin and acetylcysteine have a synergistic effect on the effect of inhibiting inflammatory factors, and the extract of inula flowers and the extract of kapok flowers can have further effects, and the two have a synergistic effect.
Test example 4
Sensory evaluation test:
160 oilpox muscle females and male volunteers with the age range of 18-40 years old are selected, randomly divided into 8 groups, 20 people in each group and men and women in each half, the products of application examples 1,4-6 and comparative application examples 1-4 are used in each group, the products are used once after facial cleaning every morning and evening, the use is continued for 60 days, other acne removing component products are not used in the period, and the use amount and the operation are ensured to be basically consistent. The mildness and vaccinia restoration effect of the product were scored after 60 days, wherein the highest score was 5 and the lowest score was 1, the scoring interval was 1-5, and the evaluation results are shown in table 4 (average score):
TABLE 4 Table 4
From table 4, it can be seen that the composition and the cosmetic prepared by the composition have excellent acne-removing and repairing properties, wherein the recombinant collagen plays an important role in improving the acne-removing and repairing effects and mildness of the product, the plant extract added into the cosmetic can further improve the acne-removing and repairing effects of the composition, and the extract of the inula flower and the extract of the kapok have a certain synergistic effect in improving the acne-removing and repairing effects of the product.
The applicant states that the technical solution of the present invention is illustrated by the above embodiments, but the present invention is not limited to the above embodiments, i.e. it does not mean that the present invention must be implemented by the above embodiments. It should be apparent to those skilled in the art that any modification of the present invention, equivalent substitution of raw materials for the product of the present invention, addition of auxiliary components, selection of specific modes, etc., falls within the scope of the present invention and the scope of disclosure.
The preferred embodiments of the present invention have been described in detail above, but the present invention is not limited to the specific details of the above embodiments, and various simple modifications can be made to the technical solution of the present invention within the scope of the technical concept of the present invention, and all the simple modifications belong to the protection scope of the present invention.
In addition, the specific features described in the above embodiments may be combined in any suitable manner, and in order to avoid unnecessary repetition, various possible combinations are not described further.
Claims (10)
1. The acne-removing repair composition containing the recombinant collagen is characterized by comprising, by mass, 4-10 parts of azelaic acid, 0.1-1 part of nisin, 0.5-5 parts of acetylcysteine and 0.01-1 part of recombinant collagen; the nucleotide sequence of the coded recombinant collagen is shown as SEQ ID NO. 1.
2. Use of the acne-removing repair composition containing recombinant collagen according to claim 1 for preparing an acne-removing repair efficacy product.
3. The use according to claim 2, wherein the acne-removing and repairing composition is added to the acne-removing and repairing efficacy product in an amount of 1-20%.
4. Use according to claim 2, wherein the product comprises a cosmetic or pharmaceutical product.
5. The gel with the acne-removing and repairing effects is characterized by comprising the following preparation raw materials in percentage by mass: the anti-acne repair composition of claim 1, 1-20%, carbomer 0.5-5%, solubilizer 0.2-2%, polyalcohol 5-20%, triethanolamine 0.1-1% and water 50-90%.
6. The gel with acne-removing and repairing effects according to claim 5, wherein the solubilizer comprises PPG-26-butanol polyether-26 and/or PEG-40 hydrogenated castor oil.
7. The gel with acne-removing and repairing effects according to claim 5, wherein the polyhydric alcohol comprises any one or a combination of at least two of propylene glycol, butylene glycol, or glycerin.
8. The gel with acne-removing and repairing effects according to claim 5, wherein the preparation raw materials of the gel further comprise, in mass percent: 1-10% of plant source conditioner, 0.05-0.5% of antioxidant or 0.1-0.5% of aromatic agent or the combination of at least two.
9. The gel with acne-removing and repairing effects according to claim 8, wherein the plant source conditioner comprises a inula flower extract and/or a kapok flower extract.
10. The method for preparing the gel with acne-removing and repairing effects according to any one of claims 5 to 9, wherein the preparation method comprises the following steps:
mixing carbomer with part of water at 25-45deg.C to obtain solution A; mixing the acne-removing repair composition, the solubilizer, the polyol and the residual water at 30-50 ℃ to obtain a solution B; mixing the solution A and the solution B at 20-40 ℃, and adding triethanolamine and the rest preparation raw materials to obtain the gel with acne removing and repairing effects.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311623336.9A CN117481997B (en) | 2023-11-29 | 2023-11-29 | Acne-removing repair composition containing recombinant collagen and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311623336.9A CN117481997B (en) | 2023-11-29 | 2023-11-29 | Acne-removing repair composition containing recombinant collagen and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN117481997A CN117481997A (en) | 2024-02-02 |
| CN117481997B true CN117481997B (en) | 2024-05-17 |
Family
ID=89684963
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311623336.9A Active CN117481997B (en) | 2023-11-29 | 2023-11-29 | Acne-removing repair composition containing recombinant collagen and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117481997B (en) |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080086229A (en) * | 2007-03-22 | 2008-09-25 | 한국콜마 주식회사 | Cosmetic compositon containing azelaic acid for treating acne skin and its manufacturing method thereof |
| CN113559105A (en) * | 2014-01-29 | 2021-10-29 | 维奥姆治疗有限公司 | Treatment of resistant acne |
| CN113797143A (en) * | 2021-10-15 | 2021-12-17 | 中国热带农业科学院农产品加工研究所 | Bromelain acne-removing essence and preparation method thereof |
| CN114028264A (en) * | 2021-11-26 | 2022-02-11 | 广州贝奥吉因生物科技股份有限公司 | Microneedle patch for acne removal and repair and preparation method and application thereof |
| CN115252460A (en) * | 2022-08-18 | 2022-11-01 | 媞颂日化用品(广州)有限公司 | Acne-removing composition containing antibacterial peptide and preparation method and application thereof |
| CN115414282A (en) * | 2022-09-21 | 2022-12-02 | 陕西慧康生物科技有限责任公司 | Composition containing ultramicro azelaic acid with acne-removing, anti-inflammatory, whitening and freckle-removing effects |
-
2023
- 2023-11-29 CN CN202311623336.9A patent/CN117481997B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20080086229A (en) * | 2007-03-22 | 2008-09-25 | 한국콜마 주식회사 | Cosmetic compositon containing azelaic acid for treating acne skin and its manufacturing method thereof |
| CN113559105A (en) * | 2014-01-29 | 2021-10-29 | 维奥姆治疗有限公司 | Treatment of resistant acne |
| CN113797143A (en) * | 2021-10-15 | 2021-12-17 | 中国热带农业科学院农产品加工研究所 | Bromelain acne-removing essence and preparation method thereof |
| CN114028264A (en) * | 2021-11-26 | 2022-02-11 | 广州贝奥吉因生物科技股份有限公司 | Microneedle patch for acne removal and repair and preparation method and application thereof |
| CN115252460A (en) * | 2022-08-18 | 2022-11-01 | 媞颂日化用品(广州)有限公司 | Acne-removing composition containing antibacterial peptide and preparation method and application thereof |
| CN115414282A (en) * | 2022-09-21 | 2022-12-02 | 陕西慧康生物科技有限责任公司 | Composition containing ultramicro azelaic acid with acne-removing, anti-inflammatory, whitening and freckle-removing effects |
Also Published As
| Publication number | Publication date |
|---|---|
| CN117481997A (en) | 2024-02-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| TWI750716B (en) | Topical composition and its use for preparation of composition for improving skin disease and dermatitis | |
| CN116003577B (en) | Recombinant collagen for skin soothing and repairing and application thereof | |
| CN110946813B (en) | Compound fermentation extract containing cubilose and preparation method and application thereof | |
| KR101734064B1 (en) | Novel antimicrobial peptide derived from myxinidin peptide and uses thereof | |
| KR101494661B1 (en) | Composition for hair or scalp treatment comprising fermented black garlic residue and method of preparing the same | |
| CN110721117A (en) | Freeze-dried powder preparation of beauty and skin care cosmetics and application of freeze-dried powder preparation in solution | |
| CN110478308A (en) | A kind of cosmetic composition of the extract containing yeast-raised products and preparation method thereof | |
| CN117481997B (en) | Acne-removing repair composition containing recombinant collagen and application thereof | |
| CN117598926A (en) | Skin barrier repair composition containing recombinant collagen and application thereof | |
| CN114404326A (en) | Moisturizing composition for pet local care and preparation method and application thereof | |
| KR20170106571A (en) | A patch formulation for preventing or improving acne comprising antibacterial adhesive peptide | |
| CN115252460B (en) | Acne-removing composition containing antibacterial peptide, and preparation method and application thereof | |
| JP2012077018A (en) | Skin collagen production-promoting agent | |
| CN109199895B (en) | Smoothie acne-removing gel and preparation method thereof | |
| CN114848721B (en) | Acne-removing composition containing plant extracts and application thereof | |
| CN105073089B (en) | Cosmetic use of Q-base | |
| CN115414308A (en) | Acne removing composition as well as preparation method and application thereof | |
| CN111297765B (en) | Roxburgh rose extract and antibacterial application thereof | |
| KR20220104655A (en) | Composition for treating acne containing nanoized bee pollen extract as an active ingredient | |
| KR101938301B1 (en) | Anti-acne composition comprising extracts of rubus coreanus, and willow or propolis | |
| CN111281977A (en) | Anti-acne composition containing lipase inhibitor and vegetable oil | |
| KR20090001268A (en) | Cosmetic composition comprising thyme oil for treating acne vulgaris | |
| WO2017113263A1 (en) | Novel use of gluconacetobacter xylinus fermentation broth as cosmetic composition | |
| KR101347250B1 (en) | A cosmetic composition comprising papain and bamboo salt | |
| CN115212148B (en) | Acne removing composition and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |