CN117462465A - Water extract of gentian, antioxidation mask mud of gentian and application thereof - Google Patents
Water extract of gentian, antioxidation mask mud of gentian and application thereof Download PDFInfo
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Abstract
The invention discloses a gentian water extract, gentian antioxidant mask mud and application thereof, and belongs to the technical field of skin care products. According to the invention, optimized parameters of the water extraction of the gentian are screened out through a test, so that the gentian water extract with excellent antioxidant activity is obtained; then a mask mud is provided, the composition of which is: 0-6 parts of water, 14-22 parts of gentian water extract, 38-42 parts of kaolin, 18-22 parts of honey, 8-12 parts of hyaluronic acid, 2-4 parts of propylene glycol, 2-4 parts of pearl powder and 3-7 parts of glycerol. The invention takes the gentiana straminea as the raw material and water as the extraction solvent, is environment-friendly, reduces the production cost, has better oxidation resistance, has better free radical scavenging activity, has better oxidation resistance, aging resistance and moisturizing effect, has scientific formula, simple process and stable and reliable quality, and indicates a new direction for developing the Chinese herbal medicine mask.
Description
Technical Field
The invention belongs to the technical field of skin care products, and particularly relates to a gentian water extract, gentian antioxidant mask mud and application thereof.
Background
The herba Hedyotidis Diffusae is annual herb of genus Hedyotis of Compositae, and is named herba Artemisiae Annuae, fel Ursi, herba Artemisiae Annuae tip, herba Gentianae etc. Mainly distributed in Yunnan, sichuan and Guizhou provinces. The gentian is rich in substances such as saponin, polysaccharide, tarragon, flavone and the like, has good antioxidation effect, is mainly applied to medical treatment at present, for example, the literature 'the research on chemical components of the gentian and antiulcer activity thereof' (Su Yanfang and the like, chinese herbal medicine, 2007) reports that the gentian can play a certain role in treating rat gastric ulcer, can inhibit proliferation of cervical cancer cells and lung cancer cells, and has a role in treating cervical cancer and lung cancer; the literature 'anti-tumor activity research of gentian total saponins' (Qin Duan, tianjin university of science and technology, 2016) reports that saponins have obvious inhibition effect on cough reaction of mice caused by an atmospheric ammonia fog method, and can inhibit the contraction of isolated tracheal spasm Li Xing caused by histamine. The development of the downstream product of the gentian is less at present, and the application range of the medicinal material is limited.
With the improvement of the social living standard, people pay more attention to skin care, the mask is a skin care product which is deeply favored and welcome at present, in the future mask market, purely natural and safe will become the main melody developed by the mask industry, artificially synthesized substances are always suspected to have safety problems, and people tend to pursue to use purely natural extracts in plants to manufacture purely natural pollution-free masks nowadays, for example, CN202310513534.3 discloses a mask with whitening, moisturizing and antioxidation functions, which is prepared by taking fresh rosa roxburghii fruits as raw materials; CN202211380937.7 discloses a facial mask with the ability of keeping moisture and scavenging free radicals, which is prepared from licorice extract and lavender extract as raw materials.
The gentian is rich in flavonoid, saponin and other antioxidant substances, and the gentian is used as a raw material to prepare the mask mud, so that the comprehensive development and utilization of gentian germplasm resources in Panxi area can be promoted, the concept of green, natural and healthy skin care is met, free radicals can be effectively eliminated by the antioxidant substances, SOD activity is improved, skin can be effectively protected, and the mask mud is anti-aging, and the antioxidation mask mud prepared by using the gentian as a raw material has important research significance and use value.
Disclosure of Invention
According to the invention, optimal parameters of the water extraction method of the gentian are screened through experiments, so that an excellent extraction effect is obtained, and based on the optimal parameters, the anti-oxidation mask mud for the water extract of the gentian is provided.
The invention firstly provides a gentian water extract, and the preparation method comprises the following steps:
A. mixing the gentian powder and water according to a mass-volume ratio of 1: mixing 12.5-17.5, extracting at 62.5-78.5 ℃, and then carrying out solid-liquid separation to obtain residue A and extract A;
B. and B, adding the residue A obtained in the step A according to the mass-volume ratio of 1:12.5 to 17.5 of water is added, extraction is carried out at the temperature of 62.5 to 78.5 ℃, and then solid-liquid separation is carried out, thus obtaining residue B and extract B;
C. and B, adding the residue A obtained in the step A according to the mass-volume ratio of 1:12.5 to 17.5 of water is added, extraction is carried out at the temperature of 62.5 to 78.5 ℃, and then solid-liquid separation is carried out, thus obtaining extract C;
D. mixing the extractive solutions A, B and C to obtain water extractive solution of herba Hedyotis Auriculariae.
Preferably, in the step A, the mass-volume ratio of the gentian water extract is controlled to be 14.5-15.5.
Preferably, in the step B, the mass-volume ratio of the gentian water extract is controlled to be 14.5-15.5.
Preferably, in the step C, the mass-volume ratio of the gentian water extract is controlled to be 14.5-15.5.
Preferably, the step A is carried out at 68.5-72.5 ℃.
Preferably, the gentian water extract is extracted at 68.5-72.5 ℃ in the step B.
Preferably, the gentian water extract is extracted at 68.5-72.5 ℃ in the step C.
Preferably, in the step A, the extraction time of the gentian water extract is 11-13 h.
More preferably, in the step A, the extraction time of the gentian water extract is 11.5-12.5 h.
Preferably, in the step B, the extraction time of the gentian water extract is 11-13 h.
More preferably, in the step B, the extraction time of the gentian water extract is 11.5-12.5 h.
Preferably, in the step C, the extraction time of the gentian water extract is 11-13 h.
More preferably, in the step C, the extraction time of the gentian water extract is 11.5-12.5 h.
Wherein, in the gentian water extract, the granularity of the gentian powder is sieved by a 60-mesh sieve.
The invention further provides the gentian antioxidant mask mud which comprises the following components in parts by mass: 0-6 parts of water, 14-22 parts of the gentian water extract, 38-42 parts of kaolin, 18-22 parts of honey, 8-12 parts of hyaluronic acid, 2-4 parts of propylene glycol, 2-4 parts of pearl powder and 3-7 parts of glycerol.
Preferably, 18-22 parts of the gentian water extract is controlled in the gentian antioxidant mask mud.
Preferably, in the gentian antioxidant mask mud, 0-2 parts of water is controlled.
The invention also provides application of the gentian antioxidant mask mud in skin care or fruit preservation.
In the invention, the mass-volume ratio of feed liquid refers to the ratio of the mass of the raw material of the gentian powder to the volume of water at the same level unit, for example, the mass-volume ratio of feed liquid is 1:12.5 to 17.5 g of gentian powder: 12.5-17.5 mL of water. In the steps B and C, the mass-volume ratio of the feed liquid is still controlled by the dosage of the raw material of the gentian powder in the step A.
The invention has the beneficial effects that:
the invention takes the gentian as raw material, water as extraction solvent, green plant extraction, safety, no pollution, environmental protection, production cost reduction and better oxidation resistance; the gentian antioxidant mask mud provided by the invention accords with the green health advocated by modern people, pursues the natural consumption concept, not only meets the requirements of the beauty-seeking person by adapting to the green environment-friendly concept, but also can deeply process and utilize Chinese herbal medicines with strong time-ordered properties; the gentian facial mask mud provided by the invention has better free radical scavenging activity, better antioxidation, anti-aging and moisturizing effects, scientific formula, simple process, stable and reliable quality and higher antioxidation activity, and a new direction is pointed out for the development of Chinese herbal medicine facial masks.
Drawings
FIG. 1 is a graph showing antioxidant activity of extracts at different extraction temperatures.
FIG. 2 is a graph showing antioxidant activity of the extracts at various extraction times.
FIG. 3 is a graph showing antioxidant activity of extracts with different feed ratios.
Fig. 4 is a graph of the scavenging rate of DPPH radicals of mask mud with different proportions.
Fig. 5 is a graph of the scavenging rate of hydroxyl radicals of mask mud with different proportions.
FIG. 6 shows facial mask mud ABTS in different proportions + Free radical scavenging rate plot.
Fig. 7 is a graph of the scavenging rate of superoxide anion radicals of mask mud with different proportions.
Fig. 8 is a graph of apple weight loss ratio for each set of mask mud.
Fig. 9 is a first day physical view of apples under each group of mask mud.
Fig. 10 is a physical image of apples under each group of mask mud on day 13.
Fig. 11 is a view of the apple sets with facial mask mud washed off after day 13.
Detailed Description
Specifically, the preparation method of the gentian antioxidant mask mud comprises the following steps:
A. mixing the gentian powder and water according to a mass-volume ratio of 1: mixing 12.5-17.5, extracting at 62.5-78.5 ℃, and then carrying out solid-liquid separation to obtain residue A and extract A;
B. and B, adding the residue A obtained in the step A according to the mass-volume ratio of 1:12.5 to 17.5 of water is added, extraction is carried out at the temperature of 62.5 to 78.5 ℃, and then solid-liquid separation is carried out, thus obtaining residue B and extract B;
C. and B, adding the residue A obtained in the step A according to the mass-volume ratio of 1:12.5 to 17.5 of water is added, extraction is carried out at the temperature of 62.5 to 78.5 ℃, and then solid-liquid separation is carried out, thus obtaining extract C;
D. mixing the extractive solutions A, B and C to obtain water extractive solution of herba Hedyotis Auriculariae.
The early test of the invention finds that the extraction is carried out for 1 time or 2 times, the extraction concentration is lower, the active ingredients in the gentiana macrophylla are wasted in a large amount, the active ingredients are basically extracted completely after 3 times of extraction, the extraction times are continuously increased, the extraction effect is basically the same as that of 3 times, and the preparation of mask mud is not facilitated due to the excessive water consumption, so the invention repeatedly extracts the gentiana macrophylla powder for three times.
In order to obtain the gentian water extract suitable for preparing mask mud, the invention carries out a large number of screening on the extraction conditions, and experiments find that: extracting at 70 ℃ for 12h with a feed-liquid ratio of 1:15, the best effect. According to the screening result, in the step A, the mass-volume ratio is controlled to be 14.5-15.5; in the step B, the mass-volume ratio is controlled to be 14.5-15.5; in the step C, the mass-volume ratio is controlled to be 14.5-15.5.
In order to obtain more excellent water extract, in the step A, the extraction is carried out at 68.5-72.5 ℃; in the step B, extracting is carried out at 68.5-72.5 ℃; in the step C, the extraction is carried out at 68.5-72.5 ℃.
Experiments show that in the step A, the extraction time is controlled to be 11-13 h; in the step B, the extraction time is controlled to be 11-13 h; in the step C, the extraction time is controlled to be 11-13 h; the active substances contained in the gentiana rigescens can achieve better extraction efficiency. Preferably, in the step A, the extraction time is controlled to be 11.5-12.5 hours; in the step B, the extraction time is controlled to be 11.5-12.5 h, and in the step C, the extraction time is controlled to be 11.5-12.5 h; the maximum extraction efficiency is reached.
All the whole herb of gentian can be used as a medicine, but leaves are generally used only in order to allow it to continue growing. According to the invention, the granularity of the gentian powder is 60 mesh.
In the step D, after the extracting solution A, the extracting solution B and the extracting solution C are combined, small particles possibly exist in the solution to influence the light absorption value, and the combined solution can be centrifuged for 10-12 min at 4000-4500 r/min.
The invention optimizes the water extraction method of the gentian so that the obtained water extraction liquid of the gentian is more suitable for preparing mask mud and has excellent antioxidant activity, and based on the method, the invention also provides the gentian antioxidant mask mud which comprises the following components in parts by mass: 0-6 parts of water, 14-22 parts of the gentian water extract, 38-42 parts of kaolin, 18-22 parts of honey, 8-12 parts of hyaluronic acid, 2-4 parts of propylene glycol, 2-4 parts of pearl powder and 3-7 parts of glycerol.
In order to further improve the oxidation resistance, water retention capacity and the like of the gentian oxidation resistance mask mud, 18-22 parts of gentian water extract is controlled; 0-2 parts of control water.
The invention also provides application of the gentian antioxidant mask mud in skin care or fruit preservation.
The present invention will be described in further detail by way of examples, which are not intended to limit the scope of the invention.
Example 1
Cleaning the picked gentian with distilled water, placing in a ventilation place, air-drying to constant weight, pulverizing with a high-speed pulverizer, sieving with a 60-mesh sieve, placing the gentian leaf powder into a dry reagent bottle, and preserving at room temperature.
1. Obtaining optimal parameters of the gentian water extract:
(1) Weighing 3 parts of 5.00g of the gentian leaf powder, putting the gentian leaf powder into a beaker, adding 50mL of deionized water, respectively carrying out water bath in a water bath kettle at 60 ℃ and 70 ℃ for 12 hours, pouring out an extracting solution, and repeating the steps twice (namely adding water into 50mL of residues after the extracting solution is poured out for extraction twice); finally, 3 times of liftingMixing the obtained solutions to obtain a gentian extract, centrifuging for 10-12 min at 4000-4500 r/min, collecting the supernatant, and measuring the pH value and ABTS of the supernatant + Scavenging rate of free radicals such as hydroxyl and superoxide anions. The results are shown in FIG. 1, and the results show that the extraction temperature is 70℃and the effect is best.
(2) 3 parts of 5.00g of the gentian leaf powder are weighed, put into a beaker, added with 50mL of deionized water, respectively subjected to water bath for 10 hours, 12 hours and 14 hours in a water bath kettle with the optimal temperature of 70 ℃ to pour out the extracting solution, and then the steps are repeated twice. Finally, mixing the extracting solutions for 3 times to obtain the gentian extracting solution, centrifuging for 10-12 min at 4000-4500 r/min, taking the supernatant, and measuring the pH value and ABTS of the supernatant + Scavenging rate of free radicals such as hydroxyl and superoxide anions. The results are shown in FIG. 2, and the measured results show that the extraction time is 12h, which is the best.
(3) Weighing 3 parts of 5.00g of the gentian leaf powder according to the mass-volume ratio of 1: 10. 1:15. 1: adding deionized water into the water bath kettle with the optimal temperature of 70 ℃ for 12 hours respectively, pouring out the extracting solution, and repeating the steps twice; finally, mixing the extracting solutions for 3 times to obtain the gentian extracting solution, centrifuging for 10-12 min at 4000-4500 r/min, taking the supernatant, and measuring the pH value and ABTS of the supernatant + Scavenging rate of free radicals such as hydroxyl and superoxide anions. The results are shown in FIG. 3, and the measured results show a feed to liquid ratio of 1:15 is most effective.
The optimal parameters of the gentian water extract obtained by the method are that the extraction temperature is 70 ℃, the extraction time is 12 hours, and the feed-liquid ratio is 1:15.
2. the optimal proportion of the gentian facial mask mud is obtained:
(1) Antioxidant activity determination of mask mud
The mask mud is prepared from deionized water, herba Hedyotis Auriculariae extractive solution, kaolinitum, mel, hyaluronic acid, propylene glycol, margarita powder, and glycerol. In the early test, according to the color proportion, viscosity and the like of mask mud, the optimal proportion of other materials except the gentian extract is found to be 20% of deionized water, 40% of kaolin, 20% of honey, 10% of hyaluronic acid, 3% of propylene glycol, 2% of pearl powder and 5% of glycerol.
And (3) respectively preparing six groups of mask mud with different proportions after obtaining the extracting solution under the optimal reference of the step (1).
A first group: deionized water 20%, kaolin 40%, honey 20%, hyaluronic acid 10%, propylene glycol 3%, pearl powder 2% and glycerol 5%;
second group: 16% of deionized water, 4% of gentian extract, 40% of kaolin, 20% of honey, 10% of hyaluronic acid, 3% of propylene glycol, 2% of pearl powder and 5% of glycerol;
third group: 12% of deionized water, 8% of gentian extract, 40% of kaolin, 20% of honey, 10% of hyaluronic acid, 3% of propylene glycol, 2% of pearl powder and 5% of glycerol;
fourth group: 8% of deionized water, 12% of gentian extract, 40% of kaolin, 20% of honey, 10% of hyaluronic acid, 3% of propylene glycol, 2% of pearl powder and 5% of glycerol;
fifth group: 4% of deionized water, 16% of gentian extract, 40% of kaolin, 20% of honey, 10% of hyaluronic acid, 3% of propylene glycol, 2% of pearl powder and 5% of glycerol;
sixth group: 20% of gentian extract, 40% of kaolin, 20% of honey, 10% of hyaluronic acid, 3% of propylene glycol, 2% of pearl powder and 5% of glycerol.
Measuring the DPPH and ABTS of the sample + Scavenging rate of free radicals such as hydroxyl and superoxide anions. The results are shown in FIGS. 4-7, and show that the aqueous extract has a DPPH radical scavenging rate, an OH radical scavenging rate, and an ABTS in the sixth set of ratios + Radical scavenging Rate and O 2 - The clearance rate of (a) is highest and is respectively 37.12%, 34.10%, 37.58% and 34.15%.
(2) Six groups of mask mud with different proportions have antioxidant effect on fresh-cut apples
Six apples were taken and grouped into six groups, one for each apple. Each apple was cut in half, the left portion of the first set of apples was coated with deionized water, and the right portion was coated with the gentian extract, as shown in fig. 9, the wrinkles and browning levels of the apples coated with the gentian extract were less than those of the left coated with deionized water, indicating that the gentian can effectively reduce the antioxidant level of the apples.
The left part of each half of the rest second to sixth apples is coated with deionized water, the right part of each half is coated with facial mask mud added with the gentian extracting solution in different proportions and facial mask mud not added with the gentian extracting solution respectively to form a comparison group, the first half is a sample group, and the second half is a comparison group. As can be seen from fig. 9 to 11, the color of the samples treated with the facial mask mud at different ratios was gradually dull with time. However, the color of the samples of each sample group is obviously higher than that of the control group, which indicates that the mask mud has an inhibition effect on the browning of apples. The comparison between the different treatment groups, the sixth group was generally superior to the other treatment groups, and the color was significantly superior to the other groups. Therefore, compared with other proportions of mask mud, the mask mud of the sixth group has the best oxidation resistance and the mask mud has the best proportion.
As can be seen from fig. 8, the weight loss ratio of the samples coated with different facial mask mud showed an increasing trend with time in the whole experimental process, but the increasing trend gradually decreased, tended to be gentle, indicating that the water loss of the apples gradually decreased. Compared with the control group, the weight loss rate of each sample group is smaller than that of the control group, the mask mud coated with the gentian extract can reduce the water evaporation of apples, the weight loss rate is reduced, the comparison is carried out among six different sample groups, and the fact that the weight loss rate of each sample group is not particularly obvious is found, the sixth group is the smallest in weight loss rate, the mask mud water retention effect of the sixth group is the most obvious, and the mask mud proportion is optimal.
The optimal proportion of the gentian antioxidant mask mud obtained by the method is 20% of gentian extract, 40% of kaolin, 20% of honey, 10% of hyaluronic acid, 3% of propylene glycol, 2% of pearl powder and 5% of glycerol.
3. And (5) evaluating the sensory properties.
The face is selected as a test part, 10 skin health college students at school are randomly selected to carry out sensory evaluation on the gentian antioxidant mask mud, and the specific evaluation process is as follows: on the test day, the subject firstly cleans the face by using clear water, and sits still for 30-35 min in a laboratory with the temperature (21+/-1) ℃ and the humidity of 50+/-10% RH; and then a proper amount of mask mud is taken and evenly smeared on the face, after 15 minutes, the mask mud is washed down, and then scoring is carried out according to the using experience. Sensory evaluation criteria are shown in the following table.
TABLE 1 sensory evaluation criteria for mask mud
The skin is kept moist after the mask mud is smeared, and the phenomena of redness, eruption, vesicular formation and the like are avoided, and the phenomena of itching, pain, allergy and the like are avoided, so that the mask mud is free from irritation. The scores of the subjects are shown in table 2 below.
Table 2 sensory evaluation of subjects
| A subject | 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | Average score |
| Scoring of | 72 | 78 | 81 | 71 | 75 | 75 | 76 | 84 | 70 | 72 | 75.4 |
From the feedback result, it can be seen that: after the sample mask mud is used, adverse reaction does not occur on the skin of an observation area, and the quality of the mask mud can be up to more than 70 minutes for a tester.
Claims (9)
1. The gentian water extract is characterized in that: the preparation method comprises the following steps:
A. mixing the gentian powder and water according to a mass-volume ratio of 1: mixing 12.5-17.5, extracting at 62.5-78.5 ℃, and then carrying out solid-liquid separation to obtain residue A and extract A;
B. and B, adding the residue A obtained in the step A according to the mass-volume ratio of 1:12.5 to 17.5 of water is added, extraction is carried out at the temperature of 62.5 to 78.5 ℃, and then solid-liquid separation is carried out, thus obtaining residue B and extract B;
C. and B, adding the residue A obtained in the step A according to the mass-volume ratio of 1:12.5 to 17.5 of water is added, extraction is carried out at the temperature of 62.5 to 78.5 ℃, and then solid-liquid separation is carried out, thus obtaining extract C;
D. mixing the extractive solutions A, B and C to obtain water extractive solution of herba Hedyotis Auriculariae.
2. The water extract of gentian according to claim 1, wherein: in the step A, the mass-volume ratio is controlled to be 14.5-15.5; in the step B, the mass-volume ratio is controlled to be 14.5-15.5; in the step C, the mass-volume ratio is controlled to be 14.5-15.5.
3. The water extract of gentian according to claim 1, wherein: in the step A, extracting is carried out at 68.5-72.5 ℃; in the step B, extracting is carried out at 68.5-72.5 ℃; in the step C, the extraction is carried out at 68.5-72.5 ℃.
4. The water extract of gentian according to claim 1, wherein: in the step A, the extraction time is 11-13 h; in the step B, the extraction time is 11-13 h; in the step C, the extraction time is 11-13 h;
preferably, in the step A, the extraction time is 11.5-12.5 h; in the step B, the extraction time is 11.5-12.5 h; in the step C, the extraction time is 11.5-12.5 h.
5. The water extract of gentian according to any one of claims 1 to 4, wherein: the granularity of the gentian powder is that the gentian powder is sieved by a 60-mesh sieve.
6. The gentian antioxidation mask mud is characterized in that: the composite material consists of the following components in parts by mass: 0 to 6 parts of water, 14 to 22 parts of the gentian aqueous extract according to any one of claims 1 to 5, 38 to 42 parts of kaolin, 18 to 22 parts of honey, 8 to 12 parts of hyaluronic acid, 2 to 4 parts of propylene glycol, 2 to 4 parts of pearl powder and 3 to 7 parts of glycerol.
7. The anti-oxidation mask mud of gentian according to claim 6, wherein: 18-22 parts of gentian water extract.
8. The anti-oxidation mask mud of gentian according to claim 6 or 7, wherein: 0-2 parts of water.
9. Use of the gentian antioxidant mask mud according to any one of claims 6 to 8 in skin care or fruit preservation.
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