CN1173642C - Oral liquid of marine organism and its preparation - Google Patents
Oral liquid of marine organism and its preparation Download PDFInfo
- Publication number
- CN1173642C CN1173642C CNB011018690A CN01101869A CN1173642C CN 1173642 C CN1173642 C CN 1173642C CN B011018690 A CNB011018690 A CN B011018690A CN 01101869 A CN01101869 A CN 01101869A CN 1173642 C CN1173642 C CN 1173642C
- Authority
- CN
- China
- Prior art keywords
- plant
- sea
- polysaccharide
- oral liquid
- aqueous solution
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The present invention relates to an oral liquid of marine organisms and a preparation method thereof. The present invention is characterized in that water-soluble polysaccharide with the molecular weight of 2000 to 650000 and polysaccharide derivatives are prepared from specific marine plants through high speed centrifugation, organic solvent precipitation, specific component degradation and chromatograph technology treatment and are mixed with required aqueous solutions conforming to GMP after being properly treated. The oral liquid can provide substrates for human body polysaccharide metabolism, can improve, supplement and strengthen the human body polysaccharide metabolism so as to enhance the immune function of a body, and is the first international commercial developed nutrition health-care functional food with marine plant polysaccharide as main structure molecules.
Description
Technical field
The invention belongs to trophism functional food technical field, relate in particular to a kind of oral liquid of marine organism and preparation method thereof.
Background technology
The existence of life not only depends on the existence of energy-food chain, also depends on the picked-up of the important active material that function is arranged.On earth in the evolution course of the For hundreds of millions of years of life, life experienced one by simple to complexity, by rudimentary to senior evolutionary process.As the highest form one people that life is evolved, have complicated evolutionary edge, the multiple biological enzymatic reaction that it had is the fundamental reaction that earns a bare living.The essential characteristic of life is to duplicate, and the people that senior thought ability, social structure and the emotion world are arranged from simple virus to complexity is like this invariably.In maintaining the basic biochemical reaction of life, various metabolic responses all need the existence of other active material except that the energy molecule.These biological active materials comprise the material of multiple trace such as vitamin, trace element, and these micro-active materials can not be synthetic by human body self, must be absorbed by the things chain by the biology in the external world, the existence of these active materials is to maintain the necessary material of vital movement.Experiment shows, human by things except that obtaining energy, also need obtain multiple oneself know and unknown micro-bioactivator to keep metabolic needs.Lack these micro-bioactivators, can cause the dysbolism of human body, and then produce functional disturbance and produce disease.Immunologic studies show that, bioactivator is for the crucial meaning of having maintained of function of immune system.Nutrition is emphasized balanced diet, emphasizes the equilibrium picked-up of COF exactly.
Tellurian life is except that virus, and the fungi of all life forms such as the bacterium of Prokaryota, Eucaryotae, plant, animal etc. all are by cellularity.Say that from biochemical composition cell is made of large biological molecule.Have four class large biological molecules to constitute the main framework of cell: (1) protein is the most important big molecule of life, also is the representative molecule that embodies main vital functions, and protein is the polymerizable molecular that is made of seed amino acid surplus about 20.(2) nucleic acid is divided into DNA (DNA) and ribonucleic acid (RNA) two classes, is the main inhereditary material of life, the amino acid sequence of the protein of the hereditary information of its coding decision simultaneously.(3) lipid is the main molecules that constitutes life framework and energy storage, and lipoid and fatty two quasi-molecules are arranged, and lipoid has different structures, can constitute lipoprotein and carbohydrate formation glycolipid etc. with protein, is one of molecule that forms the cell framework.(4) carbohydrate is divided into list (widow) sugar and polysaccharide structures, and monose is the base unit of sugar.Carbohydrate all is to be become with hydration via the carbon dioxide of chlorophyll with occurring in nature with effect by its light by green plants.Wherein single (widow) sugar is main energy-structure molecule.Polysaccharide is important structure molecule and the important function molecule that constitutes cytoskeleton, and polysaccharide is the linear or side chain polymer that is made of the monose by more than 10 that glycosidic bond connects.Polysaccharide can also constitute polysaccharide derivates with other large biological molecule, has the important physical function.A few class large biological molecules of this of human body all be by food by external world picked-up or by synthetic after the relevant monomer matrix of external world's picked-up.
Called function food, be meant except nutrition (subfunction) and the sensation (secondary function), the food that also has adjusting physiological activity (three subfunctions).Definition according to Japanese health ministry (Ministry of Public Health that is equivalent to China), functional food is meant " its composition fully shows the health defense function to people's physical efficiency, regulates the through engineering approaches food of relevant health regulatory functions such as circadian rhythm and prevent disease and promotion rehabilitation ".Functional food is divided into two classes, i.e. (1) daily functional food, be according to different healthy consumption group's (as infant, old man, student etc.) physilogical characteristics and nutritional requirement design, be intended to promote to grow or keep the through engineering approaches food of vigor and energy or performance immunological regulation and adjusting biological rhythm.(2) particular functionality food is that (as diabetic, tumor patient, the obesity etc.) that are used for special consumer groups require design, emphasizes the prevention of food and the adjusting of promotion rehabilitation.
The disease of generation and numerous food product have the reason of four aspects to promote the developmental research (1) of functional food to understand to(for) the research of disease pathogenesis have interaction relations, simultaneously food and body's immunological function etc. have the incidence of disease of modern diseases such as cardiovascular and cerebrovascular disease that confidential relation (2) aging society brings, tumour, diabetes (3) the popular understanding to food medicine and nutrition that constantly rises to improve constantly the raising of (4) whole society living-hygienic level, impel people to seek the quality that healthy lifestyles more and raising are lived.
International and domestic since the fifties developmental research multinomial be the alimentary health-care function food of main function bioactive molecule with protein, amino acid and nucleic acid.Reach " precious difficult to understand " etc. as " Wahaha " " Apollo ", up to now, the nutritive health care product of international and domestic exploitation is all carried out for the main function bioactive molecule around protein, amino acid and nucleic acid, its raw material source is various, comprises the extracts such as bone, marrow, honeycomb, land plant, fungi, bacterium, animal of animal.These alimentary health-care function food are being brought into play important effect aspect the guarantee human beings'health.But modern science can not show a candle to research to protein and nucleic acid to STUDY ON POLYSACHAROSE.Therefore polysaccharide is not developed as the important food that bioactive functions is arranged as yet up to now in the world.Existing result of study shows, " sea-plant live element " do not contain amino acid, nucleic acid and can be used as the sugared composition of list (widow) that body energy utilizes, be to contain water-soluble polysaccharide and the polysaccharide derivates thereof of molecular weight ranges between 2000-650000 more.
The structure of polysaccharide is very complicated, and different dissolubilities is arranged.Developed the extractive technique of a series of polysaccharide and derivative thereof both at home and abroad for many years, these extractive techniques change according to the character of extracting polysaccharide, but are proved to be effective technology.
According to the retrieval of technical literature, the extraction of soluble polysaccharide and derivative thereof can adopt methods such as homogenized, precipitation, centrifugal and molecular sieve chromatography post separation to realize, but wherein the removal of nucleic acid compositions, lipopolysaccharides composition also depends on the particular processing means.
Summary of the invention
The purpose of this invention is to provide a kind of oral liquid of marine organism and preparation method thereof, it is to adopt routine techniques to handle the back by the extraction to its polysaccharide and derivative component thereof specific sea-plant, prepare oral liquid of marine organism, this oral liquid of marine organism can provide the matrix of human organism's polysaccharide metabolism, improve and the metabolism of additional body polysaccharide, reach the raising body's immunological function.
For achieving the above object, the present invention takes following design: a kind of oral liquid of marine organism, and it is to pulverize after handling through the cleaning that mixes, sterilizes, oven dry with specific sea-plant; Homogenized behind the monose is taken off in degreasing again; High speed centrifugation is carried out in the plant homogenate that obtains to be handled; Collect centrifuged supernatant, add the polysaccharide component of equivalent ethanol precipitation different molecular weight; The precipitation vacuum drying obtains polysaccharide crude; Vacuum filtration is removed ethanol, will precipitate to be dissolved in the aqueous solution again, takes off albumen, decolouring; To obtaining the digestion process that solution carries out the DNA enzyme, wherein nucleic acid molecule is degraded to single nucleotides; On the molecular sieve chromatography post, this aqueous solution is carried out column chromatography purification, select the component of molecular weight between 2000-650000 to collect; Dehydration concentrates, desalination, drying; Add the aqueous solution that meets injectable water supply standard (GMP) in right amount and make, described specific sea-plant and percentage by weight thereof are: brown algae 70%, red algae 15%, agar 15%.
Described occupy specific sea-plant 70% percentage by weight the brown algae sea-plant comprise: sea-tangle 30%; Undaria pinnitafida 30%; Bulk kelp 10%.
Described red algae sea-plant is a laver.
The method for preparing above-mentioned oral liquid of marine organism comprises the steps:
(1) collects specific sea-plant and mix in proportion, the sterilization cleaning treatment;
(2) oven dry is handled;
(3) pulverize;
(4) ungrease treatment;
(5) take off monose;
(6) add NaCI in 0.2% ratio, under 4 ℃ of conditions, carried out homogenized 10 minutes through the high-speed homogenization machine;
(7) the plant homogenate that obtains was left standstill 24 hours under 4 ℃ of conditions, during stirred once in per 1 hour;
(8) under 4 ℃ of conditions, use large-scale preparative centrifugation machine that centrifugal treating is carried out in plant homogenate, 20000 rev/mins, handled 30 minutes;
(9) collect centrifuged supernatant, add the polysaccharide component of equivalent ethanol precipitation different molecular weight;
(10) will precipitate vacuum drying, obtain polysaccharide crude;
(11) vacuum filtration is removed ethanol, will precipitate to be dissolved in the aqueous solution again, takes off albumen;
(12) decolouring;
(13) solution is carried out the digestion process of DNA enzyme, wherein nucleic acid molecule is degraded to single nucleotides;
(14) on the molecular sieve chromatography post, this aqueous solution is carried out column chromatography purification, select the component of molecular weight between 2000-650000 to collect;
(15) dehydration concentrates;
(16) desalination;
(17) drying;
(18) add the aqueous solution that meets injectable water supply standard (GMP) in right amount, bottling.
Advantage of the present invention is:
1, can improve, replenish the polysaccharide metabolism of body, the matrix of body polysaccharide metabolism is provided.
2, reduce the generation of the picked-up of food cholesterol, prevention cardiovascular and cerebrovascular disease.
3, the plant polyose component can be used as immune conditioning agent, improves the function for monitoring of body immune system.
4, have active material such as the trace element and the necessary active material of biological metabolism of multiple trace, can significantly improve body's immunological function, improve cellular metabolism, various biological activity such as delaying cell aging.
The result of study that oneself has shows, " sea-plant live element " do not contain amino acid, nucleic acid and can be used as the sugared composition of list (widow) that body energy utilizes, be to contain water-soluble polysaccharide and the polysaccharide derivates thereof of molecular weight ranges between 2000-650000 more.Elderly population, obese people and normal adult crowd had prevent that cardiovascular and cerebrovascular disease, defaecation, dimension from treating that normal intestinal flora, toxin-expelling and face nourishing, minimizing fat are taken in, pre-preventing tumor, improve body immunity function, improve effects such as cellular metabolism, delaying cell aging, its effect is extensively clear and definite, and the metabolism of body, immunological regulation etc. are had in the past the incomparable advantage of functional food health products.
The specific embodiment
The invention will be further described below in conjunction with embodiment
Embodiment 1;
(1) collect specific sea-plant (directly by the undressed marine alga of marine fishing, mainly prescription and percentage by weight thereof are: brown algae 70%, wherein sea-tangle accounts for 30%, undaria pinnitafida accounts for 30%, bulk kelp accounts for 10%; The red algae laver accounts for 15%, agar accounts for 15%) through the sterilization cleaning treatment;
(2) oven dry is handled;
(3) pulverize;
(4) ungrease treatment;
(5) take off monose;
(6) add NaCI in 0.2% ratio, under 4 ℃ of conditions, carried out homogenized 10 minutes through the high-speed homogenization machine;
(7) plant homogenate was left standstill 24 hours under 4 ℃ of conditions, during stirred once in per 1 hour;
(8) under 4 ℃ of conditions, use large-scale preparative centrifugation machine that centrifugal treating is carried out in plant homogenate, 20000 rev/mins, handled 30 minutes;
(9) collect centrifuged supernatant, add the polysaccharide component of equivalent ethanol precipitation different molecular weight;
(10) will precipitate vacuum drying and obtain polysaccharide crude;
(11) vacuum filtration is removed ethanol, will precipitate to be dissolved in the aqueous solution again, takes off albumen;
(12) decolouring;
(13) solution is carried out the digestion process of DNA enzyme, wherein nucleic acid molecule is degraded to single nucleotides;
(14) on the molecular sieve chromatography post, this aqueous solution is carried out column chromatography purification, select the component of molecular weight between 2000-650000 to collect;
(15) dehydration concentrates;
(16) desalination;
(17) drying;
(18) aqueous solution of preparation 5% in the workshop that meets injectable water supply standard (GMP);
(19) on automatic assembly line, bottle 5 milliliters every bottle.
The oral liquid of marine organism that makes includes activity marine plant polyose and reactive derivative 0.25 gram thereof.
Oral liquid of marine organism of the present invention is that first in the world what carry out commercial development research is the alimentary health-care function food of primary structure molecule with the sea-plant polysaccharide.
Animal experiment study shows that the major function of this kind oral liquid comprises:
(1) can improve the polysaccharide metabolism of body, the matrix of body polysaccharide metabolism is provided;
(2) replenish the metabolism of reinforcement body polysaccharide:
(3) have defaecation and prevent constipation;
(4) reduce the generation that the food cholesterol absorbs, prevents cardiovascular and cerebrovascular disease;
(5) reduce fatty absorption (fat-reducing):
(6) keep the normal flora of enteron aisle, reduce the breeding of producing malicious metabolizing bacteria, reduce the absorption of metabolic poison, toxin-expelling and face nourishing;
(7) pre-preventing tumor takes place, and has the effect of anti-sudden change, anti-distortion;
(8) the plant polyose component can be used as immune conditioning agent, improves the function for monitoring of body rabbit epidemic disease system;
(9) active material with multiple trace can significantly improve body's immunological function as trace element and the necessary active material of biological metabolism, improves cellular metabolism, various biological activity such as delaying cell aging.
Claims (4)
1, a kind of oral liquid of marine organism is characterized in that it is to pulverize after handling through the cleaning that mixes, sterilizes, oven dry with specific sea-plant; Homogenized behind the monose is taken off in degreasing again; High speed centrifugation is carried out in the plant homogenate that obtains to be handled; Collect centrifuged supernatant, add the polysaccharide component of equivalent ethanol precipitation different molecular weight; The precipitation vacuum drying obtains polysaccharide crude; Vacuum filtration is removed ethanol, will precipitate to be dissolved in the aqueous solution again, takes off albumen, decolouring; To obtaining the digestion process that solution carries out the DNA enzyme, wherein nucleic acid molecule is degraded to single nucleotides; On the molecular sieve chromatography post, this aqueous solution is carried out column chromatography purification, select the component of molecular weight between 2000-650000 to collect; Dehydration concentrates, desalination, drying; Add the aqueous solution that meets injectable water supply standard (GMP) in right amount and make, described specific sea-plant and percentage by weight thereof are: brown algae 70%, red algae 15%, agar 15%.
2, oral liquid of marine organism according to claim 1 is characterized in that the described brown algae sea-plant that occupies specific sea-plant 70% percentage by weight comprises: sea-tangle 30%; Undaria pinnitafida 30%; Bulk kelp 10%.
3, oral liquid of marine organism according to claim 1 is characterized in that described red algae sea-plant is a laver.
4, a kind of method for preparing ocean principal goods oral liquid comprises the steps:
(1) collect specific sea-plant, its contained sea-plant and percentage by weight thereof are: brown algae 70%, red algae laver 15%, agar 15%, and the brown algae sea-plant that wherein occupies 70% percentage by weight comprises: sea-tangle 30%; Undaria pinnitafida 30%; Bulk kelp 10%; Mix the sterilization cleaning treatment in this ratio;
(2) oven dry is handled;
(3) pulverize;
(4) ungrease treatment;
(5) take off monose;
(6) add Nacl in 0.2% ratio, under 4 ℃ of conditions, carried out homogenized 10 minutes through the high-speed homogenization machine;
(7) the plant homogenate that obtains was left standstill 24 hours under 4 ℃ of conditions, during stirred once in per 1 hour;
(8) under 4 ℃ of conditions, use large-scale preparative centrifugation machine that centrifugal treating is carried out in plant homogenate, 20000 rev/mins, handled 30 minutes;
(9) collect centrifuged supernatant, add the polysaccharide component of equivalent ethanol precipitation different molecular weight;
(10) will precipitate vacuum drying and obtain polysaccharide crude;
(11) vacuum filtration is removed ethanol, will precipitate to be dissolved in the aqueous solution again, takes off albumen;
(12) decolouring;
(13) solution is carried out the digestion process of DNA enzyme, wherein nucleic acid molecule is degraded to single nucleotides;
(14) on the molecular sieve chromatography post, this aqueous solution is carried out column chromatography purification, select the component of molecular weight between 2000-650000 to collect;
(15) dehydration concentrates;
(16) desalination;
(17) drying;
(18) add the aqueous solution that meets injectable water supply standard (GMP) in right amount, bottling.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011018690A CN1173642C (en) | 2001-02-16 | 2001-02-16 | Oral liquid of marine organism and its preparation |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011018690A CN1173642C (en) | 2001-02-16 | 2001-02-16 | Oral liquid of marine organism and its preparation |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1322497A CN1322497A (en) | 2001-11-21 |
| CN1173642C true CN1173642C (en) | 2004-11-03 |
Family
ID=4652271
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011018690A Expired - Fee Related CN1173642C (en) | 2001-02-16 | 2001-02-16 | Oral liquid of marine organism and its preparation |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1173642C (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1872882B (en) * | 2005-05-30 | 2010-05-26 | 上海水产大学 | Method for preparing laver amylose |
| CN105055616A (en) * | 2015-08-12 | 2015-11-18 | 蔡婷婷 | Seaweed-containing healthcare oral liquid |
| CN105011150A (en) * | 2015-08-26 | 2015-11-04 | 缪雪峰 | Preparation technology of seaweed healthcare oral liquid |
| CN105616443A (en) * | 2016-03-31 | 2016-06-01 | 山东健科生物技术有限公司 | Marine bio-ionic liquid |
-
2001
- 2001-02-16 CN CNB011018690A patent/CN1173642C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1322497A (en) | 2001-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN1247121C (en) | Longan aril and wolfberry fruit polysaccharide health-care liquid and its preparing method and use | |
| CN1240317C (en) | Natural food ferment beverage and its production method | |
| CN1729875A (en) | Sports drink | |
| CN1701789A (en) | Nutrition replenisher containing sialic acid and its derivative | |
| CN1299724C (en) | Health care products of lucid ganoderma and their preparation | |
| CN1672540A (en) | Hypolipidemic milk powder and its prepn process | |
| CN1620919A (en) | Oxidation resistance healthcare food of fruit and vegetable , and its production method | |
| CN1173642C (en) | Oral liquid of marine organism and its preparation | |
| CN110656151A (en) | Method for improving kidney-tonifying and yang-strengthening effects of sea cucumber | |
| CN1057126C (en) | Preparation of organic selenium extracting agent by microbe | |
| CN102871132A (en) | Health care soft capsule for strengthening immunity and preparation method thereof | |
| CN100340245C (en) | Bone strengthening oral liquor and its preparing method | |
| CN1599615A (en) | Oral immunostimulation of mammals, birds, and reptiles from (1-4) linked beta -d-mannuronic acid | |
| JP4673262B2 (en) | Health food drink with mozuku extract | |
| CN1709150A (en) | Life-cultivating paste nourishing agent and its processing method | |
| CN1966655A (en) | Mulberry fruit vinegar and its production process | |
| CN1895664A (en) | Cordyceps sinensis and emu collagens peptide capsules and production thereof | |
| CN1436490A (en) | Production process of natural marine organism health food with deep sea clam worm as material | |
| CN1336126A (en) | Natural high-protein nutrient powder and its production process | |
| CN1857332A (en) | Selenium-rich hedgehog fungus extract preparation for treating colitis, gastritis and stomach cancer and its preparing process | |
| CN1242776C (en) | Worm nutritiou health oral liquid and its preparing method | |
| CN1763091A (en) | Mumia protein and its production process | |
| CN1296059C (en) | Mackefulan immune liquid | |
| CN110623184A (en) | Novel special health-care beverage for middle-aged and old people | |
| CN1203167C (en) | Wine for modulating immune from gene of ancient organism at deep sea and its production method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |