CN117356433A - Method for breeding waxy wheat and blue waxy variety by blue dwarf-male-sterile main-auxiliary double-round group selection five-garden - Google Patents

Abstract

The invention relates to the technical field of wheat breeding, and discloses a method for breeding waxy wheat and blue waxy varieties by blue dwarf male-female double-round group selection five gardens, which comprises the following steps: a waxy round selection group is established by using the blue dwarf-male-sterile wheat, a blue waxy auxiliary round selection group is synchronously established, and waxy wheat and Lan Nuo wheat varieties are bred by adopting a blue dwarf-male-sterile main and auxiliary double round selection group five-nursery method; the invention builds a blue dwarf-male-sterile glutinous rice round selection group, synchronously builds a blue glutinous rice auxiliary round selection group, adopts a blue dwarf-male-sterile main and auxiliary double round selection group five-nursery method to select glutinous wheat and Lan Nuo wheat varieties, gets rid of selecting a non-dwarf-male-sterile plant selection blue glutinous rice variety which only breaks the linkage in a main group A region, breaks the limitation of low linkage probability and few types, enriches the blue glutinous rice variety, increases the opportunity of selecting a breakthrough blue glutinous rice variety, and ensures that the selective selection of the breakthrough blue glutinous rice variety has the same probability as the selective selection of a conventional glutinous wheat variety.

Description

Method for breeding waxy wheat and blue waxy variety by blue dwarf-male-sterile main-auxiliary double-round group selection five-garden

Technical Field

The invention relates to the technical field of wheat breeding, in particular to a method for breeding waxy wheat and blue waxy varieties by double-round group selection of blue dwarf male and female parent.

Background

Waxy wheat is commonly called sticky wheat, and amylopectin is mainly accumulated in starch grains of wheat grains, and almost no amylose is produced. The application of waxy wheat is suitable for making glutinous cake and sweet dumplings, and the waxy wheat is used as a matched flour for making noodles and bread, so that the waxy wheat has better quality and good taste and can prolong the shelf life. Waxy wheat has good processing characteristics.

Blue wheat contains anthocyanin with antioxidant function in aleurone layer of endosperm, and can enrich various microelements such as zinc, iron, chromium, manganese, selenium and the like beneficial to human body from soil, so that the blue wheat has strong health care effect. Wheat is a main ration crop of people in China, is the most main food of people in northern areas of China, can solve 'hidden hunger' through main food, and eliminates health hidden danger, and is communicated with and the same as the way of advocating by world health organization and international grain and agriculture organization, and solving the problem of insufficient nutrient such as microelements and vitamins through biological reinforcement. Today, the problem of unbalanced nutrient in staple food is increasingly prominent, and the cardiovascular and cerebrovascular diseases such as obesity, hyperglycemia, hypertension, hyperlipidemia and the like caused by the unbalanced nutrient in staple food become a main disease threatening the health of people. Therefore, blue wheat which can be used as staple food and has the effects of food therapy and health care is spotlighted in the society and market.

The blue waxy wheat has the nutritional staple food health care value of the blue wheat rich in anthocyanin, zinc, iron, selenium, manganese, chromium and other nutrients, has the special processing characteristics of waxy wheat, and expands the processing and utilization range of wheat. However, the research and production of blue waxy wheat are relatively lagged, the bred blue waxy wheat has few varieties, articles and related patent applications related to the research of the breeding method and means of the blue waxy wheat are few, the situation can not meet the requirements of strong adjustment of agricultural industry structures, and the increasingly abundant requirements of people on healthy staple foods can not be met.

The invention breeds blue glutinous variety, and the main germplasm 'blue dwarf-male-sterile' utilized by the construction of alternate selection group is an iso-translocation additional line monomer with three dominant characters of blue grain (4D/4E), dwarf (Rht 10) and anther abortion (Ms 2) linked on 4D chromosome. The application of blue dwarf-male-sterile is mainly used for hybrid seed production by utilizing wheat heterosis and constructing recurrent selection groups for recurrent selection besides being used as a hybridization tool, and has the function of a wheat germplasm innovation platform. Planting 'blue dwarf-male-sterile', using a common white grain variety as pollination, forming blue grains (accounting for about 1/4) and white grains (accounting for 3/4) on the same wheat ears, sorting the blue grains, planting the wheat ears, still dwarf, sterile, and continuously using the white grain variety as pollination, wherein the pollination performance is still the same as that of the previous generation, and the wheat ears are provided with the blue grains and the white grains; the plant height of the white grains after planting and sorting is normal, anther selfing and setting are carried out, all the set seeds are white grains which are not different from common varieties, and the next generation of the white grains is still carried out after the white grains are continuously planted. However, when blue wheat is used as a male parent for pollination of 'blue dwarf male', all the seeds are blue grains, and almost all the offspring are fertile (the principle of the offspring is still under investigation), which is the practical basis for breeding blue waxy varieties by establishing blue waxy round selection pairs.

The recurrent selection is an important approach for population improvement and germplasm innovation, and although non-dwarf fertile plants breaking linkage can appear in the blue dwarf recurrent selection population, the probability of breaking linkage is low and the types are few only by the approach, however, by constructing the blue waxy recurrent selection auxiliary population with blue dwarf, all genetic types of the main population can appear in the blue waxy variety types, the blue waxy variety types are enriched, and the opportunity of breeding breakthrough blue waxy varieties is increased.

When the blue dwarf male parent is used for constructing the common wheat alternate selection group, only a plurality of group varieties are selected, and the mixed varieties are used as male parent to pollinate the blue dwarf male parent, so that the wheat alternate selection group is very simple and convenient. However, for Waxy wheat that has been genetically deleted for the three WX-A, WX-B, WX-D protein subunit genes (equivalent to having three pairs of recessive trait genes), how to construct a Waxy wheat alternate population has never been involved, moreover, the number of the existing Waxy wheat varieties in current production is small, the yield level is greatly different from that of the conventional common wheat varieties, and reports and patent applications on how to integrally improve the yield level and the comprehensive property level of the Waxy wheat by constructing a round selection group are not yet found by utilizing blue dwarf-male-sterile construction Waxy round selection groups and blue Waxy germplasm creation methods.

Disclosure of Invention

In view of the above, the invention provides a method for breeding waxy wheat and blue waxy varieties by double-round group selection of blue dwarf male and female, which comprises the following steps: and constructing a waxy round selection group by using the blue dwarf-male-sterile wheat, synchronously constructing a blue waxy round selection auxiliary group, and breeding waxy wheat and Lan Nuo wheat varieties by adopting a blue dwarf-male-sterile main-auxiliary double round selection group five-nursery method.

The five gardens are a main group A area waxy blue dwarf male-sterile seed selection garden, a main group B area waxy variety planting seed selection garden, a double group C area blue waxy variety planting seed selection garden, a subsidiary group D area blue waxy germplasm innovation garden and a subsidiary group E area blue waxy germplasm expanding planting garden.

Specifically, a main group waxy blue dwarf-male-sterile seed nursery and an isolation region of the waxy blue dwarf-male-sterile seed nursery are used as the main group A region; a waxy variety planting seed selection garden and an isolation area are used as the main group B area; selecting a seed nursery and an isolation area for planting the blue waxy variety as the double-group C area; selecting a blue dwarf-male-sterile blue glutinous rice auxiliary group and an isolation region as a blue glutinous rice auxiliary group D region; and the blue waxy auxiliary group seed selfing expanding group planting garden and the isolation area are used as the auxiliary group E area.

Further, the main group a area specifically includes: transforming the non-waxy wheat variety into blue dwarf-male-sterile wheat or using the transformed stable blue dwarf-male-sterile wheat, pollinating and hybridizing the waxy wheat into the blue dwarf-male-sterile wheat, and backcrossing;

the method comprises the steps of (1) sorting blue dwarf-male-sterile wheat and white-grain wheat in the backcross generation, identifying waxy blue dwarf-male-sterile wheat and waxy white-grain wheat by dyeing, mixing a sterile line system of a round-selected group with known quantity of waxy blue dwarf-male-sterile wheat, and mixing the waxy white-grain wheat identified by sorting into a male parent system for pollination, so as to construct a waxy blue dwarf-male-sterile round-selected group;

according to the selection method of the recurrent colony, the selection of each recurrent generation of the waxy recurrent colony is maintained, a current generation male parent is planted on one side of the blue dwarf sterile line, a superior generation male parent is planted on the other side, the overall property level of the main colony of the waxy recurrent colony is improved by selecting the recurrent generation male parent, the current generation male parent is waxy white wheat directly separated from the blue dwarf male colony, and the superior generation male parent is waxy wheat or ear in the main colony B area seed selection garden.

Further, the main group B area specifically includes: according to the selection method of the blue dwarf-male-sterile recurrent selection, white wheat separated from the selected waxy blue dwarf-male-sterile single plants is built, a waxy variety planting seed nursery is built, an isolation region of the main group B region is built, and male parents are provided for the blue dwarf-male-sterile recurrent selection group.

The double-group C region specifically comprises: screening out the blue waxy wheat bagging, hanging, marking, single harvesting, threshing and blue grain planting, autumn sowing to establish blue waxy variety planting seed selection nursery, separately establishing isolation area planting, selecting full blue grain plants, and carrying out spike selection or single plant spike row, spike line and yield comparison on the full blue grain plants until blue waxy wheat varieties are bred. The parent line selection of the secondary group D region and the parent line selection of the secondary group E region of the double group C region can sequentially enter the blue waxy variety breeding work such as the planting spike line, the yield comparison and the like of the region, and the double group C region provides excellent male parent for the blue waxy secondary group in turn.

The blue glutinous rice auxiliary group D area is specifically as follows: randomly separating a plurality of seeds from the blue-grain wheat single-plant selected by each round in the main group A as sterile lines of the blue-waxy auxiliary group D, selecting a plurality of excellent blue-waxy selection lines from the double group C to be mixed as pollination male parent of the blue-waxy auxiliary group D, and planting the seeds which are obtained by the blue-dwarf-male-sterile wheat single-plant selection in the blue-waxy auxiliary group D and enter the auxiliary group E completely without sorting; and selecting single spikes before harvesting of the male parent line in the region D, and sending the single spikes into the planting spike line in the region C of the double groups for subsequent breeding.

The waxy blue dwarf-male-sterile wheat in the secondary group E region is from the main group A region, the pollination male parent in the secondary group E region is from the double group C region, and the secondary group E region is selected round by round along with the waxy blue dwarf-male-sterile wheat in the main group A region.

Further, the subgroup E region specifically includes: seeds produced by the blue dwarf-male-sterile wheat in the blue waxy secondary group D area are not subjected to grain color sorting, the seed is directly planted in a district drill field for selfing generation, the seeds are directly harvested for re-planting and selfing expansion groups, and single spike is selected and sent to planting spike rows in the double group C area for subsequent breeding.

The invention discloses a method for breeding waxy wheat and blue waxy varieties by blue dwarf-male-sterile main-auxiliary double-round group selection five gardens, which has the beneficial effects that compared with the prior art:

when the blue dwarf male parent is used for constructing the common wheat alternate selection group, only a plurality of group varieties are selected, and the group varieties are mixed together to be used as male parent for pollinating the blue dwarf male parent, so that the wheat alternate selection group is simple and convenient. In addition, a non-dwarf fertile plant breaking linkage can appear in the region A of the main group, and a blue glutinous variety can be selected, but the probability of breaking linkage is low and the types are few only by the way, and all genetic types of the main group can appear in the blue glutinous variety types by constructing a blue glutinous wheel to select the auxiliary group, so that the blue glutinous variety types are enriched, and the opportunity of breeding breakthrough blue glutinous variety is increased.

Detailed Description

The following description of the technical solutions in the embodiments of the present invention will be clear and complete, and it is obvious that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

1. Waxy wheat and blue dwarf-male parent selection and hybridization

1.1. Alternative parent material and pre-preparation: determining that 2 parts of waxy wheat materials (Tiannuo 158 and Jinuo 200) are utilized, selecting good varieties of 14 plan groups and preparation groups, namely Jinui 22, goldrake 02-1, black horse No. 2, wheat 8, handan 6172, jinui 20, lu MAi 21, lu Mai 23, ningnong 19, shannon 20, wen nong 14, taishan 23 and Linnui 2, and using the varieties as backcross male parents to respectively carry out continuous backcross with blue dwarf male sterile lines and backcross groups of 14 parts of blue dwarf male sterile lines and backcross groups, wherein the varieties are respectively: jimai 22 blue dwarf-male, ma Koelk 02-1 blue dwarf-male, black horse No. 2 blue dwarf-male, wheat No. 8 blue dwarf-male, handan 6172 blue dwarf-male, jimai 20 blue dwarf-male, lu wheat 21 blue dwarf-male, lu Mai blue dwarf-male, ningong No. 19 blue dwarf-male, shannng 20 blue dwarf-male, wen nong 14 blue dwarf-male, taishan 23 blue dwarf-male, lin wheat No. 2 blue dwarf-male. Parental material for later population expansion was introduced and prepared: taimai 198, zheng 7698.

1.2. Sowing and building an isolation area: waxy wheat Ji Nuo 200 is selected as a backcross male parent to be hybridized and backcrossed with 10 parts of blue dwarf male parent and 10 parts of blue dwarf male parent. 10 blue dwarf-male-sterile sorted blue grains are sown, each blue dwarf-male-sterile line is 1 m 2 lines, 1 line of male parent is waxy 200 m by one side of a reserved pavement, 3 lines occupy 0.5 m, 1 m of a built partition wall and a small pavement are reserved, 2 lines of blue dwarf-male-sterile and 1 line of waxy wheat male parent combined in another rotation mode are sown at intervals of 0.6 m on the other side, 1 m of small pavement is reserved on the other side, and two cells in the forward direction are spaced by 0.5 m. The 10 varieties are divided into two rows, 5 varieties are arranged in each row, a partition wall with the wall height of 1.2 meters is built by bamboo piles, iron wires and plastic woven cloth in the middle (the middle of 0.6 meter) of the two parallel rows before heading, and a circle of partition wall is built at the position of about 25 cm outside sowing rows around the planting area. The two cells are also separated by bamboo piles and woven cloth to form a plurality of independent isolation areas, and the mixed plants in the male parent are observed to be removed in time, so that various anther plants such as the long-stalk plants separated from the blue dwarf male parent are removed in time. The waxy wheat in the flowering period is free pollination of blue dwarf, and the parent is shifted by a small bamboo pole to carry out supplementary pollination in the main pollination period.

The round selection group firstly selects Ji glutinous 200 as a waxy character source of a male parent, and 10 group varieties are selected from 10 varieties of Jimai 22, ji Koelk 02-1, heima No. 1, Mai 8, handan 6172, jimai 20, lumai 23, kangnong 19, shannon 20 and Taishan 23; especially, the corresponding blue dwarf-male sterile backcross populations are Jimai 22 blue dwarf male sterile, ji's goldenrain 02-1 blue dwarf male sterile, heima No. 1 blue dwarf male sterile, wheat No. 8 blue dwarf male sterile, handan 6172 blue dwarf male sterile, jimai 20 blue dwarf male sterile, lu Mai blue dwarf male sterile, ningnong 19 blue dwarf male sterile, shannong 20 blue dwarf male sterile, taishan 23 blue dwarf male sterile and 10 blue dwarf male sterile and backcross populations respectively.

The number of waxy varieties selected by the group is proper, the genetic basis is relatively richer, but considering the reality that the overall property level of the waxy wheat is lower at present, one waxy wheat is selected as a donor variety with waxy properties in the group.

1.3. Harvesting and sorting: and harvesting seeds (two colors of seeds of blue and white) of dwarf and male-sterile seeds in each isolation region respectively according to the isolation regions, threshing by single harvest, and sorting the blue seeds in the isolation regions to preserve and wait for sowing in autumn. The pollinated male parent is singly harvested and stored and is continuously used as the male parent.

The first step of constructing the waxy wheat wheel selection group is completed: hybridization of waxy wheat and various transgenic varieties.

2. Backcross and waxy identification

As with the previous generation, the same seeding mode and field layout are used, 2 rows of separated blue dwarf male parent and 1 row of waxy wheat backcross male parent are sowed, corresponding isolation areas are established, various separated fertile plants and hybrid plants in waxy wheat are timely pulled out, isolation walls are established by adopting the same method before heading and the previous generation, each independent isolation area is established, any waxy wheat is free pollination of blue dwarf male parent, and small bamboo poles are used for poking male parent plants for supplementary pollination in the full pollination period for improving pollination seed setting rate. The male parent and the female parent are respectively written for pollination and fruiting of the blue dwarf male parent and the female parent, and single harvesting and threshing are carried out for independent storage; waxy wheat male parent also selects typical plant wheat ears for harvesting and threshing.

And sorting the blue grains and white grains in the seeds obtained by harvesting the blue dwarf male and female. Before sowing wheat, respectively identifying the waxy property of blue grains and white grains, cutting at the coronary hair end by 2-3 mm with a surgical knife, and cutting with 0.2% I ₂ Dyeing with KI solution, eliminating all blue-black grains, and retaining embryo ends of brown grains (brown grains of waxy wheat). The waxy blue dwarf-male-sterile and waxy white grains identified for each variety can form respective waxy backcross groups, and the group scale and seed quantity of the waxy backcross groups are enlarged.

Or adopting molecular marker to assist in selection, and detecting whether the non-embryo coronary hair end lacks WX-A, WX-B, WX-D three wall protein genes.

3. Group and operation of waxy blue dwarf alternate colony

3.1. The group composition: and respectively forming a blue dwarf waxy backcross population by the identified blue grains (blue dwarf male sterile) and the waxy white grains, and expanding the population scale and the seed quantity of the blue dwarf male sterile backcross population. And mixing 10 parts of waxy blue grains and white grains after the respective group expansion to form a waxy blue dwarf system, forming a white grain waxy material from 10 backcross groups into a male parent system to form a blue dwarf waxy round selection group, and performing subsequent selection according to a method for round selection of the blue dwarf round selection group.

The more the number of backcross generations of non-waxy varieties before the group, the higher the comprehensive level of the established waxy blue dwarf-male-sterile alternate group.

3.2. And (5) field layout and sowing and coloning: sowing 6 rows of blue dwarf male parents, sowing single seeds, sowing 25 seeds/meter, sowing 1 row of uniformly mixed waxy male parents on two sides, and sowing the male parents in a wide seedling zone of about 50 seeds/meter. Small walkways with the width of 1 meter are reserved at the periphery. And (5) timely removing various anther plants separated from the blue dwarf-male-sterile population during the growth period. Before heading, building a partition wall by using bamboo piles, iron wires and plastic woven cloth, wherein the height of the wall is about 1.2 meters, and building a partition area. Free pollination is carried out in the flowering stage, and the small bamboo poles are used for poking the male parent plants for supplementary pollination in the pollination full stage so as to increase the setting rate.

3.3. Recurrent selection of waxy blue dwarf-male-sterile recurrent populations (main population a region): the field layout is the same as the above, 6 rows of blue dwarf male parent are sown, 1 row of male parent is sown on two sides (the male parent is sown in the mixed harvest of the last year, the white grains which are sorted in the blue dwarf male parent of the current year are sown on the other side), and walkways are arranged around the field layout. The isolation area is built before heading, various separated anther plants are pulled out, natural pollination or auxiliary artificial pollination is carried out in the isolation area, bad single plants are eliminated in the blue dwarf male parent in each generation, good single plants are selected and reserved, seed sorting is carried out on the collected and reserved blue dwarf male parent, blue seed planting is carried out, father is utilized in the first two rounds, white seed (called as current-generation male parent) separated from the blue dwarf current-generation selected plants is utilized on one side, and seeds (called as current-generation male parent) formed by mixing the last-year male parent are used as pollination male parent on the other side.

3.4. Establishment of main group B region of waxy wheat seed selection nursery

Starting from the first round, the white seeds (all waxy seeds) are separated, a seed nursery B area is additionally built, and isolated planting is carried out. And (3) carrying out cell mechanically-sowing thin-sowing planting on the white seeds sorted in the current year, carrying out conventional management, selecting single spikes in the group before maturity, wherein one part of the single spikes are used for the next-year seed spike row, and one part of the mixed threshing of the single spikes is used as a parent of the previous generation.

3.5. Running of main group of blue dwarf-male-sterile waxy round selection group

In the region A of the blue dwarf-male-sterile main group, the blue dwarf-male-sterile single plant is eliminated and retained in the first few rounds, and the size of the round-male-sterile main group is enlarged as soon as possible; after the blue dwarf waxy round selection population reaches a certain scale, the selection intensity of the blue dwarf is gradually enhanced, but the coverage of selection is also noted, the previous selection is changed into the selection, and then the elimination intensity of the blue dwarf selection is gradually increased.

In waxy wheat seed selection nursery (main group B area), white seeds which are selected from blue dwarf-male-sterile seeds in the current year are sown in a district, field management is adopted, ears are selected before maturation, ears are selected in the next year, and good ears are raised. The ear rows are arranged together and the ear series are arranged together in each year, and the yield comparison test is participated in by the stability and yield factors. The spike lines with excellent performance are usually bred into new waxy wheat varieties according to a systematic seed selection procedure. At present, a plurality of new waxy wheat strains with stable characters are selected from the main group B region. Over time we must be able to select more and better waxy wheat varieties.

Pollinated male parents are provided for the main round selection group A from the B region seed selection nursery every year and planted on the blue dwarf male parent side as the upper-generation male parent. Male parent composition principle: firstly, the overall character is well represented, secondly, the type is various, the genetic basis is wide, thirdly, the special character is provided, thirdly, the seed quantity of each type is smaller than the ratio, more types are stored as much as possible, and more seeds of the selected spike or spike row are selected.

The waxy wheat alternate selection population is not too small, and the minimum population size should be kept to be about half mu. The first two rounds are because the early basic seeds of the group are fewer, only individual bad single plants are generally pulled out for enlarging the group demand, most of blue dwarf-male-sterile plants are reserved, blue grain sowing is carried out, the size of the round-male-sterile group is enlarged, 6 rows of blue dwarf-male-sterile and 1 row of pollination male parent are adopted in the field layout of the large-scale round-male-sterile group, the small walkways are not reserved in the field, large-furrow planting is carried out, or the large-furrow planting is carried out in a courtyard, or relatively firm isolation walls with the height of more than 1.5 meters are built around the round-male-sterile group. The male parent selection planting area (B area) for sorting white grains can be planted in the same large isolation area, then the isolation area which is relatively slightly shorter (1.2 meters can be built), and the male parent selection nursery isolation area can be independently built at alternative places.

4. Discovery and selection of blue waxy wheat strains: after the recurrent selection of a plurality of generations, the overall character level of the waxy wheat Lan Ai abortive recurrent selection group is greatly improved, and the non-dwarf fertile plants which break the linkage and appear in the waxy blue dwarf-male sterile are searched from the recurrent selection groups, bagging and hanging (preventing pollen from polluting the blue dwarf-male sterile), after the pollination of the group is finished, the bagging is removed, the plastic rope is used for loose bagging, hanging is checked, and related information is marked in detail. And (3) in the mature period, pulling out the selected plants one by one according to the selected plants, threshing according to the plants, and sorting blue grains to be planted in the area C.

5. Establishing a blue waxy planting nursery isolation region (region C): blue grains (in theory, blue glutinous grains should be used) are sown and sorted according to the selected plants one by one, and 0.2% of I is needed before sowing ₂ Dyeing KI solution to determine whether waxy plants are selected, sowing waxy plants only), sowing single seeds, and sowing 20 seeds/m, wherein each single seedThe plant is sown in double rows, and the row length is usually 2-3 meters. And checking the color of the seeds one by one during harvesting, wherein part of the single plants have blue-white seeds, and all the seeds of part of the single plants are full blue single plants with blue seeds. Selecting and reserving all blue single plants, sowing rows of the selected all blue single plants in the next year, sowing 3 row areas, and growing the rows for 5 meters, selecting single spikes and spike building rows for the still-separated plants, carrying out subsequent breeding according to a systematic seed selection program, and simultaneously comparing the yields of the plants until the plants are stable, and breeding blue waxy wheat.

Planting the blue waxy seed selection nursery (C area) in a single courtyard or building an isolation area before heading to prevent pollen from polluting other varieties and pollen of other non-waxy varieties from polluting the group so as to lose the waxy property.

6. Establishing a blue dwarf-male-blue glutinous rice auxiliary round group D region: blue grains sorted by a plurality of blue dwarf-male-sterile plants in the main group A are taken as sterile lines, excellent blue waxy selected plants or selected lines in the blue waxy planting nursery C are taken as male parents, blue dwarf-male-sterile dynamic round selection groups are built, the sterile lines are selected round by round along with the round selection of the main round selection groups, blue dwarf-male-sterile plants are provided round by round from the main group A, the excellent blue waxy plants or spike seeds newly selected from the blue waxy seed selection nursery in the C are mixed from the auxiliary group blue waxy male parents round by round, the blue dwarf-male-sterile plants and the blue waxy male-sterile plants are dynamically updated round by round, and the character level is gradually optimized.

And (3) field arrangement and sowing of blue glutinous auxiliary wheel selection groups: and 6 rows of blue dwarf male parents selected from the main group in the area A are sown, one row of blue waxy male parents selected from the area C are sown on two sides of the main group, and the periphery of the main group is formed into an isolation area by using isolation walls (the height of the walls is about 1.3 meters) built by bamboo piles, iron wires and plastic woven fabrics before heading. Free pollination is carried out in the flowering period after heading, and blue dwarf and male parent seeds are respectively harvested after maturation.

The seeds produced in the D region of the blue dwarf-male-sterile blue glutinous rice secondary round selection group are all blue grains and are all glutinous and fertile, genetic backgrounds among individuals in the group are different from each other, the seeds are ideal groups for breeding blue glutinous rice varieties, the seeds have the potential for breeding breakthrough blue glutinous rice large varieties, not only the D region needs to be established with a separate isolation region, but also the later generation of the D region needs to be established with a separate planting isolation region E region.

The male parent seeds in the region D are all blue waxy seeds from the region C, the process of taking the male parent in the region D is also the process of stabilizing the self-copulation characters, and excellent all blue single ears are selected from the male parent and are sent into the planting ear rows in the region C again to enter the subsequent selection.

Establishment of 7.E region: the seeds produced in the blue dwarf-male-sterile blue waxy auxiliary wheel group-selecting region D do not need to be subjected to plant selection and grain color sorting, the seeds harvested in the auxiliary group-D region are directly subjected to mechanical sowing in a region E, the group is subjected to conventional field planting management, and the total region is harvested and calculated according to conventional investigation and recorded, and the relative percentage of the yield level of each round and the yield of waxy control varieties is counted, or the single spike is mixed and threshed in a planting region before maturation for the planting in a next generation region. And harvesting seeds, mechanically sowing and selfing for planting a first generation in a larger cell area, and giving more character recombination opportunities, and carrying out field management, investigation and recording and the same generation as the yield statistics. Whole blue single spikes were selected in the secondary planting population prior to maturation. And (3) single-ear threshing eliminates the selection ears with separated grain colors, not full grains and poor color and luster, bags and stores the selection ears according to single ears, and the blue glutinous rice selection ears with full blue are planted in the C area to form ear rows in the next year, and enter a systematic breeding program of the blue glutinous rice variety. The seeds produced in the C region further provide male parent for the blue waxy minor group in the D region.

By using the method, a batch of waxy wheat and Lan Nuo wheat strains are selected to participate in the variety comparison test in the unit.

In conclusion, the invention utilizes the blue dwarf-male-sterile to construct a waxy round-selection group, synchronously constructs the blue waxy round-selection group, and breeds waxy wheat and Lan Nuo wheat by adopting a blue dwarf-male-sterile main-auxiliary double round-selection group five-nursery method. The function and operation modes of each nursery of the main group and the auxiliary group are as follows:

the waxy blue dwarf-male-female alternate group main group blue dwarf-male-female and isolation region (main group A region): firstly, transferring a group of non-waxy wheat varieties into blue dwarf-male-sterile varieties (or utilizing blue dwarf-male-sterile varieties which are stable in transfer, and utilizing waxy wheat to pollinate and hybridize various blue dwarf-male-sterile varieties, backcrossing, sorting blue grains (blue dwarf-male-sterile varieties) and white grains in the backcrossing generation, identifying waxy grains (waxy blue dwarf-male-sterile varieties) and waxy white grains by dyeing, mixing a plurality of (a certain number of) waxy blue dwarf-male-sterile varieties together to form a sterile line system of a round selection group, mixing the identified waxy white grains into a male parent system for pollinating, and jointly constructing a waxy blue dwarf-male-sterile round selection group (main round selection group). And the more the number of backcross algebra of non-waxy varieties before the group, the higher the comprehensive level of the established waxy blue dwarf-male-sterile alternate group.

Operation of the main group: according to the selection method of the recurrent group, the selection of each recurrent generation of the waxy recurrent group is maintained, the current generation male parent (white grains selected by blue dwarf male sterile line selection) is planted on one side of the blue dwarf male sterile line, the upper generation male parent (excellent waxy scions selected from the main group B region seed selection nursery are mixed to be used as pollination male parent) is planted on the other side of the blue dwarf male sterile line, and the overall trait level of the main group of the waxy recurrent group is improved by the selection of the blue dwarf male sterile line.

Seed selection nursery and isolation area (main group B area) for waxy variety planting: according to the selection method of the blue dwarf-male-sterile recurrent selection, white grains (from a main group A area) separated from the selected waxy blue dwarf-male-sterile single plants are built, waxy variety planting seed selection gardens are built, independent isolation areas (B areas) are built, male parents are provided for the blue dwarf-male-sterile recurrent selection groups, and particularly excellent single plants are selected to be bred into waxy wheat varieties according to a systematic seed selection program.

Seed selection nursery and isolation area (double group C area) for planting blue glutinous rice variety: the method is characterized in that when a round selection group of the technical system is utilized to select and breed common white waxy wheat varieties, blue waxy fertile plants (with normal plant heights and anthers) separated by breaking linkage are searched from a round selection group (A area) of a round selection group of waxy blue dwarf, bagging and hanging label single-harvest threshing are carried out, blue grain planting is carried out, autumn sowing is carried out, a blue waxy variety planting seed selection garden is established, and isolation area (C area) planting is independently established. Selecting full blue plants from the wheat, then carrying out variety stabilization and yield comparison work such as ear row selection (or single plant) planting, ear line selection, yield comparison and the like on the plants according to a systematic breeding program until blue waxy wheat varieties are bred, wherein the C area is simultaneously responsible for providing father for blue waxy auxiliary groups round by round.

The whole blue selection spike selected in the subsequent subsidiary group E area and the single spike selected in the subsidiary group D area father line also enter the C area to plant spike line and spike line, and the single spike and the blue plant from the A area participate in test and comparison at the same time, so the double group C area is called.

And (3) selecting a group and an isolation region (a blue waxy sub group D region) by a blue dwarf-male-female blue waxy sub group: and randomly separating out a part of seeds from blue grains (blue dwarf-male-sterile lines from the A group) selected by each blue dwarf-male-sterile single plant of each round of the main group of the waxy blue dwarf-male-sterile line, selecting a plurality of excellent blue waxy lines from a blue waxy variety planting seed selection nursery (double group C area) to be mixed as a pollination male parent of the auxiliary group, and planting the seeds in an isolation area E which is independently set for the seeds without sorting all the seeds. And (3) selecting a single spike of a blue glutinous population with excellent male parent behaviors in the region, feeding the single spike into a planting spike row in the region C, and carrying out subsequent blue glutinous variety breeding.

The waxy blue dwarf-male-sterile of the blue waxy auxiliary group is from the region A (the waxy blue dwarf-male-sterile of the main group), the pollination male parent of the blue waxy auxiliary group is from each preferred blue waxy line of the region C, the auxiliary group is selected round by round along with the waxy blue dwarf-male-sterile of the main group A, the character level of the auxiliary group is more and more excellent round by round, the character level of the father parent is also better along with the selection of a blue waxy seed selection nursery (the region C), and the population overall level is also improved round by round along with the selection of the blue waxy seed selection nursery (the region C).

Blue waxy auxiliary group seed selfing expanding group planting nursery and isolation area (auxiliary group E area): seeds produced by the current generation of blue dwarf-male-sterile in the region D (blue waxy variety planting seed selection group) are not subjected to grain color sorting, the self-bred generation of the field drill field planting is directly adopted, the seeds are directly harvested for carrying out the field planting, or single spike mixing and threshing are carried out, the field drill field planting is carried out again, all-blue single plants or all-blue single spikes are selected, the spike rows and the spike lines are planted in the region C (blue waxy variety planting seed selection nursery and isolation region), and subsequent selection is carried out, wherein the preferable spike lines become male parents of the region D (blue dwarf-male-sterile blue waxy variety seed selection group and isolation region) in the next round.

The embodiment of the invention uses germplasm conditions to explain:

and (3) carrying out natural glutinous rice 158: the method is characterized in that the method comprises the steps of breeding of Toyo Tianyi biological limited company, approval in 2011, wherein the approval number is Yu-to-be-approved wheat 2011016, and the Zibo lover seed industry limited company is collected and provided.

Ji glutinous 200: hebei province institute of agriculture and forestry science, institute of grain and oil crops, 3 months of 2011, hebei province approval, approval number Ji-to-examine wheat 2011002. The Zibo lover seed industry limited company is collected and provided.

Jimai 22: the Shandong province agricultural academy of sciences crop institute. In 2006, the approval numbers are determined by the Shandong province and the country approval at the same time: lu Nong No. 2006050 and No. 2006018. The Zibo lover seed industry limited company is collected and provided.

Valencia 02-1: the method is characterized by breeding in the original seed field of Koelreuteria county at the university of Hebei province. Approval in Hebei province 2004, approval number is Ji-to-trial wheat 2004009); in 2007, the national Huang-Huai-Bei tablet is approved, and the approval number is national audit wheat 2007016. The Zibo lover seed industry limited company is collected and provided.

Black horse No. 1: the Shandong state black horse seed industry Limited company is bred, the Shandong province approval in 2004, and the approval number (Lu Nong audit word 2004 No. 026. Zibo loving people seed industry Limited company is collected and provided).

Black horse No. 2: the Shandong state black horse seed industry limited company is bred, the Shandong province approval in 2006 is Lu Nong to 2006055, and the Zibo lover seed industry limited company is collected and provided.

wheat 8: the breeding of the academy of agricultural science in the city of Fangfang is carried out by approval in Shandong province in 2003, and the approval number is: lu Nong the word is examined [2003] 028. The Zibo lover seed industry limited company is collected and provided.

Handan 6172: the Shandon agricultural academy of sciences of Handan in Hebei province breeds, and the national Huang-Huai-Bei-pian approval in 2003 is the national trial wheat 2003036. The Zibo lover seed industry limited company is collected and provided.

Jimai 20: the Shandong province institute of agricultural science developed, 8 months in 2003 passed the Shandong province approval, approval number: lu Nong the word [2003] 029. The Zibo lover seed industry limited company is collected and provided.

Lu Mai 23: the Shandong black horse seed industry limited company and the victory oilfield horse mill farm cooperate and select a seed, and in 1996, the Shandong province approval is passed, and the approval number is the Lu seed audit number 0201. The Zibo lover seed industry limited company is collected and provided.

Lu Mai 21: and (3) breeding by using a tobacco stand city academy of agricultural sciences, and in 1996, checking the tobacco stand city of Shandong province, wherein the checking number is Lu-seed checking number 0199. The Zibo lover seed industry limited company is collected and provided.

Tobacco grower No. 19: the breeding of the agricultural sciences of the tobacco stand city is examined and approved in Shandong province in 2001, the examined and approved number is Lu Nong examined word No. 2001, and the Zibo lover seed industry Limited company is collected and provided.

Shannong 20: breeding at Shandong agricultural university, passing national Huang-Huai nan-piece approval in 2010, wherein the approval number is national audit wheat 2010006. The approval number of 2011 is national audit wheat 2011012 through national Huang-Huai-Bei tablet approval. Zibo lover seed industry Limited company collection and provision

Taishan No. 23: the Taian national academy of sciences was bred, and in 2004, was approved by Shandong province, and Lu Nong was reviewed in [2004] 023. Zibo lover seed industry Limited company collection and provision

Linmai No. 2: breeding of Linyi city academy of agricultural sciences, shandong province approval in 2004, approval number: lu Nong the words of the word 2004 021 are collected and provided by the company of the seed industry of Bo lovers.

Venturi farm 14: the Wen agriculture seed industry Limited liability company of Taian city breeds, 2011 passes the national Huang-Huai North tablet approval, and the approval number is Guo-Bao-Mai 2011015. Approval by Shandong province 2010, lu Nong, 2010068, collection and provision by Zibo loving min seed industry Limited.

Ether-wheat 198: the Taishan region Jiu and crop research institute of Taian, shandong province, the approval of Shandong province in 2016, the approval number is Lu-to-Be 20160056, the Zibo lover seed industry Limited company is collected and provided.

Zhengmai 7698: the wheat research center of the agricultural academy of sciences of Henan province is used for breeding, and the wheat is examined and examined 2011008 in 2011; the country approval of Huang-Huai-nan-nationality in 2012 is that the approval number is national approval wheat 2012009, which is collected and provided by Zibo lover seed industry Limited company.

The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.

Claims (7)

1. A method for breeding waxy wheat and blue waxy varieties by double-round group selection of blue dwarf male and female parent is characterized by comprising the following steps: and constructing a waxy round selection group by using the blue dwarf-male-sterile wheat, synchronously constructing a blue waxy auxiliary round selection group, and breeding waxy wheat and Lan Nuo wheat varieties by adopting a blue dwarf-male-sterile main and auxiliary double round selection group five-nursery method.

2. The method for breeding waxy wheat and blue waxy varieties by double-round group selection of blue dwarf-male-sterile type according to claim 1, wherein the five gardens are a main group A area waxy blue dwarf-male-sterile type seed selection garden, a main group B area waxy type planting seed selection garden, a double group C area blue waxy type planting seed selection garden, a secondary group D area blue waxy type innovation garden and a secondary group E area blue waxy type expanding group planting garden.

Specifically, a main group waxy blue dwarf-male-sterile seed nursery and an isolation region of the waxy blue dwarf-male-sterile seed nursery are used as the main group A region; a waxy variety planting seed selection garden and an isolation area are used as the main group B area; selecting a seed nursery and an isolation area for planting the blue waxy variety as the double-group C area; selecting a blue dwarf-male-sterile blue glutinous rice auxiliary group and an isolation region as a blue glutinous rice auxiliary group D region; and the blue waxy auxiliary group seed selfing expanding group planting garden and the isolation area are used as the auxiliary group E area.

3. The method for breeding waxy wheat and blue waxy varieties by double-round group selection of blue dwarf male-female-male-female varieties according to claim 2, wherein the main group A area is specifically: transferring the non-waxy wheat variety into blue dwarf-male-sterile or using the transferred stable blue dwarf-male-sterile, and crossing and backcrossing the waxy wheat variety into blue dwarf-male-sterile;

the method comprises the steps of (1) sorting blue grains (blue dwarf-male-sterile) and white grains in the backcross generation, identifying waxy blue dwarf-male-sterile and waxy white grains by dyeing, mixing a sterile line system of a waxy round-robin colony formed by mixing known waxy blue dwarf-male-sterile wheat and the waxy white grains identified by sorting into a male parent system for pollination, and constructing a waxy blue dwarf-male-sterile round-colony;

according to the selection method of the recurrent colony, the selection of each recurrent generation of the waxy recurrent colony is maintained, a current generation male parent is planted on one side of the blue dwarf sterile line, a last generation male parent is planted on the other side, the overall property level of the main colony of the waxy recurrent colony is improved by selecting the recurrent, the current generation male parent is waxy white wheat directly sorted out from the blue dwarf male colony, and the last generation male parent is waxy selective plant or selective spike and spike line seed in the main colony B area seed selection garden.

4. The method for breeding waxy wheat and blue waxy varieties by double-round group selection of blue dwarf male and female parent according to claim 3, wherein the main group B area is specifically: according to the selection method of the blue dwarf-male-sterile recurrent selection, white wheat separated from the selected waxy blue dwarf-male-sterile single plants is built, a waxy variety planting seed nursery is built, an isolation region of the main group B region is built, and male parents are provided for the blue dwarf-male-sterile recurrent selection group.

5. The method for breeding waxy wheat and blue waxy varieties by double-round group selection of blue dwarf male-sterile main and auxiliary, according to claim 4, characterized in that the double-group C region is specifically: screening out the blue waxy wheat bagging, hanging, marking, single harvesting, threshing and blue grain planting, autumn sowing to establish blue waxy variety planting seed selection nursery, separately establishing isolation area planting, selecting full blue grain plants, and carrying out spike selection or single plant spike row, spike line and yield comparison on the full blue grain plants until blue waxy wheat varieties are bred. The parent line selection of the secondary group D region and the parent line selection of the secondary group E region of the double group C region can sequentially enter the blue waxy variety breeding work such as the planting spike line, the yield comparison and the like of the region, and the double group C region provides excellent male parent for the blue waxy secondary group in turn.

6. The method for breeding waxy wheat and blue waxy varieties by double round of selecting five gardens of blue dwarf male-sterile main and auxiliary rounds of selecting group according to claim 5, the method is characterized in that the blue waxy auxiliary group D area is specifically as follows: randomly separating a plurality of seeds from the blue-grain wheat single-plant selected by each round in the main group A as sterile lines of the blue-waxy auxiliary group D, selecting a plurality of excellent blue-waxy selection lines from the double group C to be mixed as pollination male parent of the blue-waxy auxiliary group D, and planting the seeds which are obtained by the blue-dwarf-male-sterile wheat single-plant selection in the blue-waxy auxiliary group D and enter the auxiliary group E completely without sorting; and selecting single spikes before harvesting of the male parent line in the region D, and sending the single spikes into the planting spike line in the region C of the double groups for subsequent breeding.

The waxy blue dwarf-male-sterile wheat in the secondary group E region is from the main group A region, the pollination male parent in the secondary group E region is from the double group C region, and the secondary group E region is selected round by round along with the waxy blue dwarf-male-sterile wheat in the main group A region.

7. The method for breeding waxy wheat and blue waxy varieties by double round of selecting five gardens of blue dwarf male-sterile main and auxiliary rounds of selecting group according to claim 6, the method is characterized in that the secondary group E area is specifically as follows: seeds produced by the blue dwarf-male-sterile wheat in the blue waxy secondary group D area are not subjected to grain color sorting, the seed is directly planted in a district drill field for selfing generation, the seeds are directly harvested for re-planting and selfing expansion groups, and single spike is selected and sent to planting spike rows in the double group C area for subsequent breeding.