CN117342893A - Salicylic acid iron fertilizer for improving stress resistance of plants and preparation method thereof - Google Patents
Salicylic acid iron fertilizer for improving stress resistance of plants and preparation method thereof Download PDFInfo
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- CN117342893A CN117342893A CN202311253099.1A CN202311253099A CN117342893A CN 117342893 A CN117342893 A CN 117342893A CN 202311253099 A CN202311253099 A CN 202311253099A CN 117342893 A CN117342893 A CN 117342893A
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- salicylic acid
- ferrous sulfate
- iron
- plants
- stress resistance
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 16
- FHXGZTHZFMMNCP-UHFFFAOYSA-N 2-hydroxybenzoic acid;iron Chemical compound [Fe].OC(=O)C1=CC=CC=C1O FHXGZTHZFMMNCP-UHFFFAOYSA-N 0.000 title description 7
- 238000002360 preparation method Methods 0.000 title description 3
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 136
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 82
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 68
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 62
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims abstract description 38
- 239000011790 ferrous sulphate Substances 0.000 claims abstract description 37
- 229910052742 iron Inorganic materials 0.000 claims abstract description 37
- 235000003891 ferrous sulphate Nutrition 0.000 claims abstract description 31
- 229950001102 salicylsulfuric acid Drugs 0.000 claims abstract description 6
- 150000001875 compounds Chemical class 0.000 claims abstract description 5
- 239000013589 supplement Substances 0.000 claims abstract description 5
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical compound O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 33
- ABBQHOQBGMUPJH-UHFFFAOYSA-M Sodium salicylate Chemical compound [Na+].OC1=CC=CC=C1C([O-])=O ABBQHOQBGMUPJH-UHFFFAOYSA-M 0.000 claims description 11
- 229960004025 sodium salicylate Drugs 0.000 claims description 11
- 230000001502 supplementing effect Effects 0.000 claims description 5
- 150000004688 heptahydrates Chemical class 0.000 claims description 3
- 150000003839 salts Chemical class 0.000 abstract description 8
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 abstract description 3
- 231100000331 toxic Toxicity 0.000 abstract description 3
- 230000002588 toxic effect Effects 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 34
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 26
- 241000220223 Fragaria Species 0.000 description 21
- 235000016623 Fragaria vesca Nutrition 0.000 description 21
- 235000011363 Fragaria x ananassa Nutrition 0.000 description 21
- 238000011282 treatment Methods 0.000 description 20
- 229930002875 chlorophyll Natural products 0.000 description 19
- 235000019804 chlorophyll Nutrition 0.000 description 19
- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 description 19
- 230000035882 stress Effects 0.000 description 18
- 230000000694 effects Effects 0.000 description 14
- 239000011780 sodium chloride Substances 0.000 description 13
- 238000012360 testing method Methods 0.000 description 11
- 230000012010 growth Effects 0.000 description 8
- 239000001963 growth medium Substances 0.000 description 7
- 230000036579 abiotic stress Effects 0.000 description 6
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
- 238000013329 compounding Methods 0.000 description 4
- 230000006378 damage Effects 0.000 description 4
- BSYNRYMUTXBXSQ-UHFFFAOYSA-N Aspirin Chemical compound CC(=O)OC1=CC=CC=C1C(O)=O BSYNRYMUTXBXSQ-UHFFFAOYSA-N 0.000 description 3
- 206010022971 Iron Deficiencies Diseases 0.000 description 3
- 241000124033 Salix Species 0.000 description 3
- 208000027418 Wounds and injury Diseases 0.000 description 3
- 229960001138 acetylsalicylic acid Drugs 0.000 description 3
- 230000003203 everyday effect Effects 0.000 description 3
- 208000014674 injury Diseases 0.000 description 3
- 230000001954 sterilising effect Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 description 2
- 206010042209 Stress Diseases 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 238000010306 acid treatment Methods 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 239000013522 chelant Substances 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 229910001385 heavy metal Inorganic materials 0.000 description 2
- 229910000360 iron(III) sulfate Inorganic materials 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000007721 medicinal effect Effects 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012452 mother liquor Substances 0.000 description 2
- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 230000035790 physiological processes and functions Effects 0.000 description 2
- 230000008635 plant growth Effects 0.000 description 2
- 239000003375 plant hormone Substances 0.000 description 2
- 230000000607 poisoning effect Effects 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 230000005855 radiation Effects 0.000 description 2
- 229960001860 salicylate Drugs 0.000 description 2
- 230000035939 shock Effects 0.000 description 2
- 239000002689 soil Substances 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- JVXHQHGWBAHSSF-UHFFFAOYSA-L 2-[2-[bis(carboxylatomethyl)amino]ethyl-(carboxylatomethyl)amino]acetate;hydron;iron(2+) Chemical compound [H+].[H+].[Fe+2].[O-]C(=O)CN(CC([O-])=O)CCN(CC([O-])=O)CC([O-])=O JVXHQHGWBAHSSF-UHFFFAOYSA-L 0.000 description 1
- 208000024827 Alzheimer disease Diseases 0.000 description 1
- 241000209524 Araceae Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- 235000008733 Citrus aurantifolia Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- CWYNVVGOOAEACU-UHFFFAOYSA-N Fe2+ Chemical compound [Fe+2] CWYNVVGOOAEACU-UHFFFAOYSA-N 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 244000141359 Malus pumila Species 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 244000061176 Nicotiana tabacum Species 0.000 description 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 1
- 208000018737 Parkinson disease Diseases 0.000 description 1
- 206010037660 Pyrexia Diseases 0.000 description 1
- 235000011941 Tilia x europaea Nutrition 0.000 description 1
- PSFYAUFLWVLVSR-UHFFFAOYSA-N [acetyloxy-[6-(diacetyloxyamino)hexyl]amino] acetate Chemical compound CC(=O)ON(OC(C)=O)CCCCCCN(OC(C)=O)OC(C)=O PSFYAUFLWVLVSR-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000000202 analgesic effect Effects 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000021016 apples Nutrition 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 208000026106 cerebrovascular disease Diseases 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 230000034994 death Effects 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000002526 effect on cardiovascular system Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 229960002413 ferric citrate Drugs 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- BMBUUNTZYQWADI-UHFFFAOYSA-N hexane-1,6-diamine;sodium Chemical compound [Na].NCCCCCCN BMBUUNTZYQWADI-UHFFFAOYSA-N 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 150000002500 ions Chemical class 0.000 description 1
- NPFOYSMITVOQOS-UHFFFAOYSA-K iron(III) citrate Chemical compound [Fe+3].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O NPFOYSMITVOQOS-UHFFFAOYSA-K 0.000 description 1
- 231100000636 lethal dose Toxicity 0.000 description 1
- 239000004571 lime Substances 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000008121 plant development Effects 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- YGSDEFSMJLZEOE-UHFFFAOYSA-M salicylate Chemical compound OC1=CC=CC=C1C([O-])=O YGSDEFSMJLZEOE-UHFFFAOYSA-M 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000011664 signaling Effects 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035924 thermogenesis Effects 0.000 description 1
- 230000000476 thermogenic effect Effects 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 238000004383 yellowing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
- C05D9/00—Other inorganic fertilisers
- C05D9/02—Other inorganic fertilisers containing trace elements
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a functional iron fertilizer for improving stress resistance of plants, belonging to the technical field of compound fertilizers and application thereof. The compound fertilizer containing salicylic acid and ferrous sulfate is characterized by containing salicylic acid which is a component for improving stress resistance of plants and ferrous sulfate which supplements iron. The weight ratio of the two is 1:27.8 to 1.15:1. the invention has the following advantages: 1. the synergy is obvious. 2. Avoiding or delaying the toxic action of single excessive use. 3. Relieving salt stress and improving stress resistance of plants.
Description
Technical Field
The invention relates to the field of plant fertilizers, in particular to a salicylic acid iron fertilizer for improving stress resistance of plants and a preparation method thereof, and belongs to the technical field of fertilizers and application thereof.
Background
The use of Salicylic Acid (SA) has a long history, and as early as 4 th century before the first trimester, the Greek physician uses willow bark as a pain killer; edmund Stone in the united kingdom 1763 describes in detail the medical effect of willow bark, which can treat malaria and fever; scientists in 1838 found that the truly active substance in willow bark was salicylic acid. In 1898, the advent of the salicylic acid, aspirin, has been widely used because salicylic acid overcomes the disadvantage of irritating intestines and stomach. Aspirin has been present for over a century from now on, and today, with highly developed science and technology, people have not abandoned this ancient medicine, but have found many new and more important medical effects, ranging from initial anti-inflammation, antipyresis, analgesic, to prevention and treatment of cardiovascular and cerebrovascular diseases, and recently discovered salicylic acid and aspirin have been effective in treating cataracts, cancers, alzheimer's disease, parkinson's disease, and their effects are not uniform.
In contrast, salicylic acid, while produced in plants, has not been noted until the 60 s of the 20 th century for its physiological function in plants. Van Herk in 1937 suggested that the thermogenesis of the Buddha flame inflorescence, a plant of Araceae, might be caused by a thermogenic element, and Lee and Skoog reported in 1965 that salicylic acid could induce flowering in tobacco group-cultivated plants. Experiments in Raskin et al have demonstrated that this thermogen is salicylic acid until 1987. Salicylic acid has now become a recognized signaling molecule involved in plant disease resistance. In terms of abiotic stress, most results show that salicylic acid is against abiotic stress such as: the abiotic stress such as heavy metal, ozone, ultraviolet radiation, low temperature, heat shock, water deficiency, salt damage and the like also has a relieving effect. Salicylic acid also has irreplaceable effects in delaying aging of fresh cut flowers and fruits. In addition, salicylic acid has been reported to regulate the closure of air holes, influence the absorption of ions by root systems, induce adventitious roots, promote the growth of lateral roots and promote the germination of side buds of new shoots of apples.
Iron is one of 16 nutrient elements necessary for plant growth and development, is abundant in soil, but has low solubility, particularly in alkaline or lime soil, and the effectiveness of iron is low, so that the requirement of plant growth is difficult to meet, and iron deficiency of plants is caused. About 40% of cultivated land worldwide has the potential for iron deficiency, and plant-derived foods are the main source of human iron, so that the iron content level of crops directly affects human health.
At present, in tissue culture, multi-purpose chelate iron, such as ferric citrate or ferric hexamethylenediamine tetraacetate, is used as an iron source, salicylic acid is known to form chelate with iron, and earlier test results of the inventor show that salicylic acid-ferrous sulfate is used as an iron source, the absorption and utilization efficiency of the salicylic acid-ferrous sulfate is between ferrous sulfate and EDTA-ferrous sulfate, and compared with EDTA-ferrous sulfate, poisoning phenomenon is not easy to occur.
Disclosure of Invention
The invention aims to provide a functional fertilizer for improving stress resistance of plants and supplementing iron elements. The invention is based on researching salicylic acid and iron on the basis of regulating plant resistance and physiological functions, further researching the application of salicylic acid and iron in the agricultural field, and particularly, the invention is completed aiming at iron deficiency prevention and treatment research of fruit trees.
According to the invention, the reasonability of the use of different compounding proportions is evaluated by researching the independent use of novel plant hormones of salicylic acid and ferrous sulfate and the indoor test of the strawberry tissue culture seedlings after compounding, and the compounding effect of the salicylic acid and the ferrous sulfate is discussed, so that the purposes of improving the stress resistance of plants, increasing the effectiveness of the ferrous sulfate, reducing the use amount, reducing the production cost, avoiding or delaying the occurrence of single excessive use poison and the like are achieved.
The technical scheme adopted by the invention is as follows: the functional fertilizer contains salicylic acid and ferrous sulfate for improving stress resistance of plants, wherein the molar mass ratio of the salicylic acid to the ferrous sulfate=1: 16-2: 1, a step of; the optimal molar mass ratio as iron supplement is 1:6, the optimal molar mass ratio of the substances for improving the stress resistance of the plants is 1:1. because salicylic acid is indissolvable in water, the solution adopts sodium salicylate and ferrous sulfate heptahydrate to compound, and the weight ratio is 1:27.8 to 1.15:1, if the sodium salicylate is used as an iron element supplement after being compounded: the optimal weight ratio of the heptahydrate to the ferrous sulfate is 1:10.5, sodium salicylate if emphasis is placed on increasing plant resistance: the optimal weight ratio of the heptahydrate to the ferrous sulfate is 1:1.7.
the compound functional fertilizer containing sodium salicylate and ferrous sulfate provided by the invention has outstanding effects in supplementing plant iron and improving the salt resistance of crops, and also has a certain relief effect on plant abiotic stress.
1. The synergy is obvious.
Ferrous sulfate alone has the effect of supplementing iron nutrients, but has poor utilization efficiency, the effectiveness of ferrous sulfate is greatly improved after sodium salicylate is added, the iron content of plants is closely related to chlorophyll, and the chlorophyll content of ferrous sulfate salicylate with the concentration of 0.2mmol/L is equivalent to that of ferrous sulfate alone with the concentration of 0.8mmol/L by taking chlorophyll as an evaluation index. CAT and POD enzymes related to stress reach the maximum value at the concentration of salicylic acid and ferrous sulfate of 0.05mmol/L, and ferrous sulfate alone reaches the maximum value at the concentration of 0.8 mmol/L. The use amount of ferrous sulfate is reduced by more than 75% by compounding.
2. Avoiding or delaying the toxic action of excessive use.
Salicylic acid is used as a plant hormone, has a characteristic of the hormone that the plant is promoted when the content is low, and can be damaged when the concentration exceeds a certain concentration. After the sodium salicylate and the ferrous sulfate are compounded and used, the existence of iron avoids or delays the toxic action caused by the pure salicylic acid. If the iron content in the strawberry culture medium is low (0.05 mmol/L), 0.2mmol/L salicylic acid causes obvious injury to strawberry tissue culture seedlings, and when the iron content in the culture medium is increased to 0.8mmol/L, 0.8mol/L salicylic acid can cause obvious injury to strawberry tissue culture seedlings. SA treatment of 0.4mmol/L increases with FeSO4 concentration (0.05-0.4 mmol/L), root length, plant height and chlorophyll content.
3. Relief of abiotic stress
After salicylic acid and iron are compounded and used, the stress of strawberry tissue culture Miao Yan can be obviously relieved, and the strain height, fresh weight and chlorophyll content are increased. Salicylic acid also has relieving effect on abiotic stress such as heavy metal, ozone, ultraviolet radiation, low temperature, heat shock, water deficiency, salt injury, etc.
Drawings
Fig. 1: strawberry tissue culture seedlings are treated with sodium hexamethylenediamine tetraacetate-ferrous sulfate (EDTA-FeSO) 4 ) Ferrous sulfate (FeSO) 4 ) Salicylic acid ferrous sulfate (SA-FeSO) 4 ) Salicylic acid-ferric sulfate [ Fe 2 (SO 4 ) 3 –SA]Chlorophyll content under treatment;
fig. 2: adding Salicylic Acid (SA) strawberry seedling growth condition diagrams with different concentrations under NaCl stress, wherein the growth condition diagrams are as follows in sequence from left to right: control (0 mmol/L NaCl+0mmol/L SA), 50mmol/L NaCl+0mmol/LSA, 50mmol/L NaCl+0.025mmol/LSA, 50mmol/L NaC+0.05mmol/L SA, 50mmol/L NaCl+0.1mmol/L SA, 50mmol/L NaCl+0.2mmol/L SA, 50mmol/L NaCl+0.4mmol/L SA;
Detailed Description
The present invention is illustrated in detail by the following specific examples, which are not intended to limit the scope of the invention in any way.
Example 1 Effect of different iron on chlorophyll content of strawberry
1.1 test methods
Respectively preparing sodium edetate-ferrous sulfate (EDTA-FeSO) 4 ) Ferrous sulfate (FeSO) 4 ) Salicylic acid ferrous sulfate (SA-FeSO) 4 ) Salicylic acid-ferric sulfate [ Fe 2 (SO 4 ) 3 –SA]Mother liquor with the concentration of 10mmol/L, different amounts of other elements with reference to the formula of MS culture medium are added in different treatments, 30g/L of sucrose and 6g/L of agar are added, and the pH value is adjusted to 5.8 to prepare the culture medium. After sterilizing at 121 ℃ for 20 minutes, inoculating the proliferated strawberry seedling buds on an ultra-clean workbench, and inoculating three strains per bottle after six bottles are treated. And (3) placing the inoculated strain into a tissue culture room, wherein the indoor culture temperature is 25+/-1 ℃, and the strain is irradiated for 14 hours every day, and the irradiation intensity is about 2000 Lux.
1.2 harvesting of test Material
The tissue culture seedlings grow for about 40 days, are harvested, the root number of 5 seedlings is measured, the plant height is measured, and the length of the longest 3 roots of each seedling is recorded. Then the root, stem and leaf are separated, quick frozen by liquid nitrogen and stored in an ultra-low temperature refrigerator at the temperature of minus 65 ℃.
1.3 analysis of results
Effect of different iron treatments on chlorophyll content of strawberry seedlings, see FIG. 1
FeSO 4 Treating strawberry seedlings, wherein the chlorophyll content is always in an ascending trend along with the increase of the iron concentration, and reaches the maximum value at 0.8 mmol/L; EDTA-FeSO 4 Treating strawberry seedlings with iron concentration of 0.05mmol/L and 0.1mmol L -1 When the chlorophyll content is significantly higher than other treatments, but then drops sharply; feSO 4 Chlorophyll content of SA treatment lower than EDTA-FeSO at iron concentration of 0.05 and 0.1mmol/L 4 The treatment was carried out, but the concentration was always rising and did not decrease. Fe (Fe) 2 (SO 4 ) 3 The chlorophyll content of the SA-treated strawberry seedlings is obviously lower than that of EDTA-FeSO at the iron concentration of 0.05 and 0.1mmol/L 4 And FeSO 4 SA treatment, but up to 0.2mmol/L and FeSO 4 The SA treatment is not so much different, as Fe 2 (SO 4 ) 3 Increasing concentration of SA, chlorophyll content is always increasing.
From the test, the combination of the ferrous salicylate and the ferrous sulfate is most suitable for being used as the supplement of the iron element.
Example 2 sodium salicylate and ferrous sulfate Compare screening
2.1 reagent for test
Sodium salicylate and ferrous sulfate (FeSO4.7H2O) are prepared into mother liquor with concentration of 10mmol/L by adopting analytically pure medicines and distilled water for standby.
2.2 test materials
The strawberry variety to be tested is Hani, the first batch of aseptic seedlings is provided by the gardening line of the Laiyang academy of agriculture, the culture medium used for proliferation is MS culture medium, 3 percent of sucrose, 6g/L of agar, 0.3-m g/L of 6-BA, 0.1 g/L of IBA and 5.8 of pH value are added, and the proliferation culture is carried out for about 20 days, so that the sprouting is used for the test. Salicylic acid and iron were added to the medium at various concentrations. The propagated strawberry seedling buds are inoculated on an ultra-clean workbench, six bottles are treated, and three strains are inoculated in each bottle. And (3) placing the inoculated strain into a tissue culture chamber, wherein the indoor culture temperature is 25+/-10 ℃, and the light is irradiated for 14 hours every day, and the light intensity is about 2000 Lux.
The tissue culture seedlings grow for about 40 days, are harvested, the root number of 5 seedlings is measured, the plant height is measured, and the length of the longest 3 roots of each seedling is recorded. Then the root, stem and leaf are separated, quick frozen by liquid nitrogen and stored in an ultra-low temperature refrigerator at the temperature of minus 65 ℃.
2.3 test treatments
The test treatments were ferrous sulfate treatments at different salicylic acid concentrations.
2.4 test results
SA is added under different iron concentrations, so that the rooting quantity is increased; the effect on root length is to remove FeSO at a concentration of 0.05mmol/L 4 0.1 to 0.8mmol/L FeSO outside the treatment 4 Adding SA with different concentrations to inhibit root elongation; the influence on plant height is that the increase of plants is promoted at low concentration of SA, and the growth is inhibited at high concentration; the change trend of chlorophyll is the same as the plant height. If compared transversely, 0.4mmol/L SA treatment followed by FeSO 4 The concentration (0.05-0.4 mmol/L) is increased, the root length, the plant height and the chlorophyll content are increased, and the survival rate is increased. The chlorophyll content is used as an index for evaluating the iron condition in plants, and salicylic acid can be added into a culture medium to promote the utilization of iron at low concentration, especially at low level of feed water; high concentrations inhibit iron utilization, leading to plant yellowing and even albino death. However, as the level of iron supply increases, the lethal concentration of salicylic acid also increases. The poisoning effect of SA with high concentration can be partially alleviated by the increase of the iron content of plants.
TABLE 1 rooting number of treatment with iron salicylic acid of different concentrations
TABLE 2 root length (cm) of treatment with iron salicylic acid at various concentrations
TABLE 3 plant height (cm) of treatment with iron salicylic acid at different concentrations
TABLE 4 chlorophyll content of iron salicylic acid treatments at different concentrations (mg.g -1 FW).
TABLE 2.5 survival rate T of high concentration salicylic acid different iron treated strawberry seedlings
Example 3 salicylic acid relief strawberry tissue culture Miao Yan stress
3.1 test method:
salicylic acid (salicylic acid SA) and sodium chloride (NaCl) were added to the medium before sterilization. After sterilizing at 121 ℃ for 20 minutes, inoculating the proliferated strawberry seedling buds on an ultra-clean workbench, and inoculating three strains per bottle after six bottles are treated. And (3) placing the inoculated strain into a tissue culture chamber, wherein the indoor culture temperature is 25+/-10 ℃, and the light is irradiated for 14 hours every day, and the light intensity is about 2000 Lux.
3.2 data statistics
The tissue culture seedlings are grown for 50 days, harvested, the fresh weight of 5 seedlings is measured, the plant height is measured, and the length of the longest 3 roots of each seedling is recorded.
3.3 test results
Physiological effects of salicylic acid on strawberry seedlings under 50mmol/L NaCl stress: the stress effect of 50mmol/L NaCl is not obvious in 0-15 days, all treatment differences of adding 0.025-0.4 mmol/L SA are not obvious, the 50mmol/L NaCl slowly shows stress symptoms in 15-30 days, and SA with the concentration of 0.025, 0.05, 0.1, 0.2 and 0.4mmol/L is added under the condition of 50mmol/L NaCl, so that the stress relieving effect is obvious, wherein the growth vigor of plants treated by 0.05 and 0.1mmol/L SA even exceeds that of a control without salt stress. The growth of salt stress plus SA after 30 days was progressively inferior to that of the blank. Harvested at 50 days. Its external form is shown in FIG. 2
The salt stress seriously hinders the growth of strawberry tissue culture seedlings, and 0.025-0.4 mmol/L salicylic acid is added under 50mmol/LNaCl stress, so that the salt stress symptoms are obviously relieved, and the root length, the plant height, the fresh weight and the chlorophyll content are increased.
Effect of salicylic acid treatment on strawberry growth and chlorophyll content under 50mmol/LNaCL stress
Claims (3)
1. The functional fertilizer contains salicylic acid for improving plant stress resistance and ferrous sulfate for supplementing iron elements, and adopts sodium salicylate and ferrous sulfate heptahydrate as compound fertilizer sources, wherein the molar mass ratio of the sodium salicylate to the ferrous sulfate heptahydrate is 1: 16-2: 1, the weight ratio is 1:27.8 to 1.15:1.
2. the functional fertilizer containing salicylic acid and ferrous sulfate according to claim 1, wherein the optimal molar mass ratio of the functional fertilizer to the iron supplement is 1:6, the optimal weight ratio is 1:10.5; to improve plant stress resistance, sodium salicylate: the optimal molar mass ratio of the heptahydrate to the ferrous sulfate is 1:1, the optimal weight ratio is 1:1.7.
3. the functional fertilizer containing salicylic acid and ferrous sulfate as set forth in claim 1 or 2, which is useful for supplementing iron element and improving stress resistance of plants.
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