CN117337722A - Culture medium for grifola frondosa cultivar and preparation method thereof - Google Patents
Culture medium for grifola frondosa cultivar and preparation method thereof Download PDFInfo
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- CN117337722A CN117337722A CN202311555651.2A CN202311555651A CN117337722A CN 117337722 A CN117337722 A CN 117337722A CN 202311555651 A CN202311555651 A CN 202311555651A CN 117337722 A CN117337722 A CN 117337722A
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- scraps
- grifola frondosa
- powder
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- 240000001080 Grifola frondosa Species 0.000 title claims abstract description 71
- 235000007710 Grifola frondosa Nutrition 0.000 title claims abstract description 71
- 239000001963 growth medium Substances 0.000 title claims abstract description 65
- 238000002360 preparation method Methods 0.000 title claims abstract description 33
- 241000223025 Caragana microphylla Species 0.000 claims abstract description 78
- 239000000843 powder Substances 0.000 claims abstract description 78
- 239000000203 mixture Substances 0.000 claims abstract description 53
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 44
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 44
- 239000010440 gypsum Substances 0.000 claims abstract description 32
- 229910052602 gypsum Inorganic materials 0.000 claims abstract description 32
- 239000003864 humus Substances 0.000 claims abstract description 32
- 239000002689 soil Substances 0.000 claims abstract description 31
- 239000002023 wood Substances 0.000 claims abstract description 31
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 28
- 230000001954 sterilising effect Effects 0.000 claims abstract description 25
- 239000002994 raw material Substances 0.000 claims abstract description 24
- 241000609240 Ambelania acida Species 0.000 claims abstract description 23
- 240000000111 Saccharum officinarum Species 0.000 claims abstract description 23
- 235000007201 Saccharum officinarum Nutrition 0.000 claims abstract description 23
- 239000010905 bagasse Substances 0.000 claims abstract description 23
- 239000002893 slag Substances 0.000 claims abstract description 23
- 238000004659 sterilization and disinfection Methods 0.000 claims description 12
- 239000000428 dust Substances 0.000 claims description 8
- 238000000034 method Methods 0.000 claims description 5
- -1 bran Substances 0.000 claims 1
- 241000233866 Fungi Species 0.000 abstract description 16
- 239000002154 agricultural waste Substances 0.000 abstract description 3
- 230000000052 comparative effect Effects 0.000 description 17
- 235000019764 Soybean Meal Nutrition 0.000 description 4
- 239000011159 matrix material Substances 0.000 description 4
- 238000012986 modification Methods 0.000 description 4
- 230000004048 modification Effects 0.000 description 4
- 239000004455 soybean meal Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000000926 separation method Methods 0.000 description 3
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 2
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- 229930003451 Vitamin B1 Natural products 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000004075 alteration Effects 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000004021 humic acid Substances 0.000 description 2
- 235000012054 meals Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 239000013630 prepared media Substances 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 239000011691 vitamin B1 Substances 0.000 description 2
- 235000010374 vitamin B1 Nutrition 0.000 description 2
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 244000028550 Auricularia auricula Species 0.000 description 1
- 235000000023 Auricularia auricula Nutrition 0.000 description 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 240000008397 Ganoderma lucidum Species 0.000 description 1
- 235000001637 Ganoderma lucidum Nutrition 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 229920002488 Hemicellulose Polymers 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000001727 Tropicoporus linteus Species 0.000 description 1
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000002246 antineoplastic agent Substances 0.000 description 1
- 229940041181 antineoplastic drug Drugs 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000003337 fertilizer Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229920005610 lignin Polymers 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 235000020238 sunflower seed Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G18/00—Cultivation of mushrooms
- A01G18/20—Culture media, e.g. compost
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Mycology (AREA)
- Environmental Sciences (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Abstract
The invention relates to the technical field of edible fungus culture, and discloses a culture medium for grifola frondosa cultivars and a preparation method thereof, wherein the culture medium comprises the following raw materials in parts by weight: 69-100 parts of wood chips, 24-36 parts of corncobs, 15-22 parts of bran, 18-27 parts of bean pulp, 1-4 parts of gypsum, 9-15 parts of humus soil and 16-20 parts of bagasse. The preparation method comprises the following steps: preparing caragana microphylla scraps, apple branch scraps, corncob powder and bean pulp powder; uniformly mixing the prepared caragana microphylla scraps, apple branch scraps, corncob powder, sugarcane slag powder, bean pulp powder, bran and gypsum in a formula amount, and adding water to obtain a mixture; and (5) subpackaging and sterilizing the mixture to obtain the culture medium of the grifola frondosa cultivar. The invention prepares the culture medium of the grifola frondosa cultivar by taking agricultural wastes as raw materials, the cultured hyphae are white, compact and vigorous, the hypha growth speed is high, the bag filling time of the hypha is early, the growth period of the cultivar is shortened, and the cost is saved.
Description
Technical Field
The invention belongs to the technical field of edible fungus culture, and particularly relates to a culture medium for grifola frondosa cultivars and a preparation method thereof.
Background
Grifola frondosa is an edible fungus integrating nutrition, delicacy and health, and the fresh fruiting body contains more than 20% of protein, more than 50% of carbohydrate and rich flavor amino acids. The polysaccharide component in the grifola frondosa is developed into an anti-tumor drug, which is one of edible fungi with drug word size processing products in China. In recent years, the research shows that the grifola frondosa polysaccharide has the effect of reducing blood sugar, and is the strongest in various edible and medicinal fungi such as ganoderma lucidum, corious versicolor, phellinus linteus and the like.
In edible fungus strain production, mycelium pure cultures obtained by fruiting body tissue separation and spore separation or mycelium separation in auricularia auricula and mushroom wood bases are generally called mother seeds, and transferred strains are also called primary seeds. The pure culture of mycelium obtained by mother seed transplanting and expanding culture is called stock seed, also called secondary seed. The stock is mainly used for producing cultivars, and can also be directly used as cultivars. Pure mycelium cultures obtained by transplanting and expanding culture of stock seeds are called cultivars, or production (used) seeds, also called tertiary seeds, and the cultivars are directly used for cultivation. The cultivation seed production aim is: 1) Further expands the amount of the strain and saves the strain cost; 2) The strain is quickly adapted to a culture formula, growth conditions and nutrients are provided for the quick growth of mycelium of the strain, and the mycelium growth time and cultivation period are shortened.
The prior art discloses a high-yield grifola frondosa culture medium and a preparation method thereof (refer to Chinese patent CN 108925366A), wherein the culture medium comprises 15-25 parts of bran, 10-15 parts of leaves, 10-15 parts of wood dust, 5-10 parts of sandy loam, 5-10 parts of rice bran, 10-15 parts of cotton seed hulls, 15-25 parts of soybean meal, 1-2 parts of glucose, 3-5 parts of sunflower seed meal, 2-4 parts of peanut meal, 0.1-0.3 part of folic acid and 60-130 parts of water. Although the patent increases the yield, the hypha growth rate is not increased and the growth cycle is shortened.
Disclosure of Invention
The invention discloses a culture medium for grifola frondosa cultivars and a preparation method thereof, and solves the problems that the growth speed of hyphae is not improved and the growth period is shortened when the grifola frondosa culture medium is prepared in the prior art.
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 69-100 parts of wood chips, 24-36 parts of corncobs, 15-22 parts of bran, 18-27 parts of bean pulp, 1-4 parts of gypsum, 9-15 parts of humus soil and 16-20 parts of bagasse.
Preferably, the culture medium consists of the following raw materials in parts by weight: 80-90 parts of wood dust, 28-32 parts of corncob, 18-19 parts of bran, 22-24 parts of bean pulp, 2-3 parts of gypsum, 11-13 parts of humus soil and 17-19 parts of bagasse.
Preferably, the culture medium consists of the following raw materials in parts by weight: 85 parts of wood chips, 30 parts of corncobs, 19 parts of bran, 23 parts of bean pulp, 3 parts of gypsum, 12 parts of humus soil and 18 parts of bagasse.
Preferably, the wood chips are a mixture of caragana microphylla chips and apple branch chips.
Preferably, the mass ratio of caragana microphylla to apple branch scraps=1:1 in the mixture.
The second purpose of the invention is to protect the preparation method of the culture medium of the grifola frondosa cultivar, which comprises the following steps:
crushing the caragana microphylla according to the formula amount to obtain caragana microphylla scraps;
the preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps;
uniformly mixing the prepared caragana microphylla scraps, apple branch scraps, corncob powder, sugarcane slag powder, bean pulp powder, and the formula amount of bran, gypsum and humus soil, and adding water to obtain a mixture;
and (5) subpackaging and sterilizing the mixture to obtain the culture medium of the grifola frondosa cultivar.
Preferably, the caragana microphylla is crushed to 80-100 meshes.
Preferably, the water is added when the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder, the bean pulp powder, the bran, the gypsum and the humus soil are mixed, so that the water content of the mixture is 30-40% by mass.
Preferably, when the mixture is sterilized, the sterilization condition is 120-130 ℃ for 100-140 min.
Compared with the prior art, the invention has the beneficial effects that:
1. the culture medium for the grifola frondosa cultivar is prepared by taking agricultural wastes as raw materials, has the advantages of low cost, easily available raw materials, simple preparation and rich nutrition, and the mycelium cultured by the culture medium for the grifola frondosa cultivar is white, dense and vigorous, has high mycelium growth speed and early bag filling time of the mycelium, shortens the growth period of the cultivar and saves the cost.
2. The invention converts lignin, cellulose and hemicellulose in the caragana microphylla by utilizing the enzymolysis of the edible fungus mycelia, and the caragana microphylla is used as a matrix raw material to culture the mycelia, so that the mycelia promote the rapid degradation of the caragana microphylla.
3. In the invention, the soybean meal is used as a matrix raw material and a source of vitamin B1 to accelerate the growth of mycelium. Bagasse is used as a raw material, contains a large amount of beneficial bacteria and active substances, such as water-soluble humic acid and crude protein, and enhances the fertility of a matrix.
4. When the culture medium for grifola frondosa cultivars is prepared, caragana microphylla, apple branches, corncobs, bran, bean pulp and bagasse in raw materials are all agricultural wastes, so that the preparation cost is reduced, and the environmental pollution is reduced.
Drawings
Fig. 1 is a flowchart of preparation of a culture medium for a grifola frondosa cultivar provided in embodiment 1 of the present invention.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely, and it is apparent that the described embodiments are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Bagasse used in the examples and comparative examples of the present invention was purchased from the company of the guangxi Mingfuxin sugar industry, inc.; bean pulp is purchased from Hebei Rundong fertilizer company; bran was purchased from Hua Mo (Shandong) biological Co., ltd; humus is purchased in the mineral processing plant of the Yongshun county of life.
Example 1
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 69 parts of wood chips, 24 parts of corncobs, 15 parts of bran, 18 parts of bean pulp, 1 part of gypsum, 9 parts of humus soil and 16 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
The preparation method of the culture medium for the grifola frondosa cultivar specifically comprises the following steps with reference to fig. 1:
s1, crushing the caragana microphylla to 80 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder, the bean pulp powder, the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 30% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 102.97kPa, 120 ℃ and 140min to obtain the culture medium of the grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Example 2
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 69 parts of wood chips, 24 parts of corncobs, 15 parts of bran, 18 parts of bean pulp, 1 part of gypsum, 9 parts of humus soil and 16 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 80 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder, the bean pulp powder, the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 30% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 103.4kPa, 121.3 ℃ and 120min to obtain the culture medium of the grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Example 3
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 80 parts of wood dust, 28 parts of corncob, 18 parts of bran, 22 parts of bean pulp, 2 parts of gypsum, 11 parts of humus soil and 17 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 80 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder and the bean pulp powder prepared in the S1 with the bran, the gypsum and the humus soil in the formula amount, and adding water to ensure that the water content ratio of the mixture is 30% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 103.4kPa, 121.3 ℃ and 120min to obtain the culture medium of the grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Example 4
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 85 parts of wood chips, 30 parts of corncobs, 19 parts of bran, 23 parts of bean pulp, 3 parts of gypsum, 12 parts of humus soil and 18 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 100 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder and the bean pulp powder prepared in the S1 with the bran, the gypsum and the humus soil in the formula amount, and adding water to ensure that the water content ratio of the mixture is 35%, thereby obtaining the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 103.4kPa, 121.3 ℃ and 120min to obtain the culture medium of the grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Example 5
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 90 parts of wood chips, 32 parts of corncobs, 19 parts of bran, 24 parts of bean pulp, 3 parts of gypsum, 13 parts of humus soil and 19 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 100 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder, the bean pulp powder, the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 40% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 137.3kPa, 126 ℃ for 100min to obtain the grifola frondosa cultivar culture medium. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Example 6
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 69 parts of wood chips, 24 parts of corncobs, 15 parts of bran, 18 parts of bean pulp, 1 part of gypsum, 9 parts of humus soil and 16 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 80 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder, the bean pulp powder, the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 30% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 137.30kPa, 126 deg.C, and 100min to obtain culture medium for grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Example 7
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 100 parts of wood dust, 36 parts of corncob, 22 parts of bran, 27 parts of bean pulp, 4 parts of gypsum, 15 parts of humus soil and 20 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 100 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the sugarcane slag powder, the bean pulp powder, the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 40% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 137.30kPa, 126 deg.C, and 100min to obtain culture medium for grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Comparative example 1
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 100 parts of wood dust, 36 parts of corncob, 22 parts of bran, 27 parts of bean pulp, 4 parts of gypsum, 15 parts of humus soil and 20 parts of bagasse.
The wood chips are apple branch chips.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the apple branches to 100 meshes according to the formula amount to obtain apple branch scraps.
The preparation method of the corncob powder and the bean pulp powder is the same as that of apple branch scraps.
S2, uniformly mixing the apple branch scraps, the corncob powder, the sugarcane slag powder, the bean pulp powder, the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 40% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 103.4kPa, 121.3 ℃ and 120min to obtain the culture medium of the grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Comparative example 2
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 100 parts of wood dust, 36 parts of corncob, 22 parts of bran, 4 parts of gypsum, 15 parts of humus soil and 20 parts of bagasse.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 100 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder and sugarcane slag powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the bagasse powder and the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 40% to obtain the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 103.4kPa, 121.3 ℃ and 120min to obtain the culture medium of the grifola frondosa cultivar. Fungus bags of 17cm×33cm×0.05cm are packed separately, and each bag weighs 1200g.
Comparative example 3
The culture medium for the grifola frondosa cultivar comprises the following raw materials in parts by weight: 100 parts of wood dust, 36 parts of corncob, 22 parts of bran, 27 parts of bean pulp, 4 parts of gypsum and 15 parts of humus soil.
The wood chips are a mixture of caragana microphylla scraps and apple branch scraps; the mass ratio of caragana microphylla scraps to apple branch scraps=1:1.
A preparation method of a culture medium for grifola frondosa cultivars specifically comprises the following steps:
s1, crushing the caragana microphylla to 100 meshes according to the formula amount to obtain caragana microphylla scraps.
The preparation methods of apple branch scraps, corncob powder and bean pulp powder are the same as those of caragana microphylla scraps.
S2, uniformly mixing the caragana microphylla scraps, the apple branch scraps, the corncob powder, the bean pulp powder, the bran, the gypsum and the humus soil with the formula amount, and adding water to ensure that the water content ratio of the mixture is 40%, thereby obtaining the mixture.
S3, subpackaging and sterilizing the mixture, wherein the sterilization conditions are as follows: 103.4kPa, 121.3 ℃ and 120min to obtain the culture medium of the grifola frondosa cultivar. The bags of 17cm ×33× 33cm ×0.05cm were packed separately, and each bag weighed 1200g.
And (3) result detection:
grifola frondosa stock was inoculated into the media prepared in examples 1-7 and comparative examples 1-3, respectively, on an ultra clean bench. Culturing in dark at 25deg.C for 20 days, and measuring growth rate of Maitake Mushroom cultivars in different culture media by streaking method, wherein the results are shown in the following table:
TABLE 1 average growth Rate of hyphae inoculated into different groups of prepared media by Grifola frondosa cultivars
| Group of | Average growth rate of hypha (mm/d) |
| Example 1 | 4.2±0.2 |
| Example 2 | 3.9±0.1 |
| Example 3 | 4.1±0.1 |
| Example 4 | 4.0±0.2 |
| Example 5 | 4.3±0.2 |
| Example 6 | 4.6±0.1 |
| Example 7 | 4.4±0.1 |
| Comparative example 1 | 3.5±0.1 |
| Comparative example 2 | 2.9±0.2 |
| Comparative example 3 | 3.2±0.2 |
The culture substrates of the grifola frondosa cultivars prepared in examples 1 to 7 and comparative examples 1 to 3 were sown, managed until harvest, and harvested when the caps were flattened and the fruiting bodies were mature.
TABLE 2 yields of Grifola frondosa cultured in different groups of Medium
| Group of | Grifola frondosa yield (g/bag) |
| Example 1 | 210 |
| Example 2 | 215 |
| Example 3 | 213 |
| Example 4 | 209 |
| Example 5 | 211 |
| Example 6 | 218 |
| Example 7 | 216 |
| Comparative example 1 | 143 |
| Comparative example 2 | 124 |
| Comparative example 3 | 137 |
As can be seen from tables 1 and 2, the average growth rate of hyphae in the culture mediums prepared in examples 1 to 7 is much faster than that in comparative examples 1 to 3, the growth rate of hyphae is fast, the feeding speed is fast, the bag filling time of hyphae is early, and the growth period of cultivars is shortened; the yield of Grifola frondosa was also higher than that of comparative examples 1 to 3. Compared with examples 1-7, the comparative example 1 has no caragana microphylla added, the average growth speed of hyphae in the prepared culture medium is slower, and the yield of the grifola frondosa is lower, which indicates that the caragana microphylla can promote the growth of the hyphae and improve the yield of the grifola frondosa; compared with examples 1-7, the comparative example 2 has no soybean meal, the average growth speed of hyphae in the prepared culture medium is very slow, and the yield of the grifola frondosa is low, which shows that the soybean meal is used as a source of vitamin B1, thereby accelerating the growth of the hyphae and improving the yield of the grifola frondosa; compared with examples 1 to 7, comparative example 3 was free of bagasse, and the average growth rate of mycelia in the prepared medium was slightly slow and the yield was low, because sugar contained in bagasse was used as a carbon source, the carbon-nitrogen ratio was adjusted, the growth of mycelia was promoted, and the yield of Grifola frondosa was increased. Bagasse contains a large amount of beneficial bacteria and active substances, such as water-soluble humic acid and crude protein, and enhances the fertility of the matrix.
While preferred embodiments of the present invention have been described, additional variations and modifications in those embodiments may occur to those skilled in the art once they learn of the basic inventive concepts. It is therefore intended that the following claims be interpreted as including the preferred embodiments and all such alterations and modifications as fall within the scope of the invention.
It will be apparent to those skilled in the art that various modifications and variations can be made to the present invention without departing from the spirit or scope of the invention. Thus, it is intended that the present invention also include such modifications and alterations insofar as they come within the scope of the appended claims or the equivalents thereof.
Claims (9)
1. The culture medium for the grifola frondosa cultivar is characterized by comprising the following raw materials in parts by weight: 69-100 parts of wood chips, 24-36 parts of corncobs, 15-22 parts of bran, 18-27 parts of bean pulp, 1-4 parts of gypsum, 9-15 parts of humus soil and 16-20 parts of bagasse.
2. The culture medium for grifola frondosa cultivars according to claim 1, wherein the culture medium comprises the following raw materials in parts by weight: 80-90 parts of wood dust, 28-32 parts of corncob, 18-19 parts of bran, 22-24 parts of bean pulp, 2-3 parts of gypsum, 11-13 parts of humus soil and 17-19 parts of bagasse.
3. The culture medium for grifola frondosa cultivars according to claim 1, wherein the culture medium comprises the following raw materials in parts by weight: 85 parts of wood chips, 30 parts of corncobs, 19 parts of bran, 23 parts of bean pulp, 3 parts of gypsum, 12 parts of humus soil and 18 parts of bagasse.
4. A culture medium for grifola frondosa cultivars according to claims 1-3, wherein the wood chips are a mixture of caragana microphylla chips and apple branch chips.
5. The culture medium for grifola frondosa cultivars according to claim 4, wherein the mass ratio of the mixture is caragana microphylla chip to apple branch chip=1:1.
6. The method for preparing a culture medium for a grifola frondosa cultivar according to claim 5, comprising the following steps:
crushing the caragana microphylla according to the formula amount to obtain caragana microphylla scraps;
the preparation methods of apple branch scraps, corncob powder, sugarcane slag powder and bean pulp powder are the same as those of caragana microphylla scraps;
uniformly mixing the prepared caragana microphylla scraps, apple branch scraps, corncob powder, sugarcane slag powder, bean pulp powder, and the formula amount of bran, gypsum and humus soil, and adding water to obtain a mixture;
and (5) subpackaging and sterilizing the mixture to obtain the culture medium of the grifola frondosa cultivar.
7. The method for preparing a culture medium for a grifola frondosa cultivar according to claim 6, wherein the caragana microphylla is crushed to 80-100 mesh.
8. The method for preparing a culture medium for a grifola frondosa cultivar according to claim 6, wherein the water is added when the caragana microphylla scraps, apple branch scraps, corncob powder, sugarcane slag powder, bean pulp powder, bran, gypsum and humus soil are mixed, so that the water content of the mixture is 30-40% by mass.
9. The method for preparing a culture medium for a grifola frondosa cultivar according to claim 6, wherein when the mixture is sterilized, the sterilization conditions are as follows: 102.97-137.30 kPa, 120-126 ℃ and 100-140 min.
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| CN106242856A (en) * | 2016-08-30 | 2016-12-21 | 贵州瑞泰农业科技有限公司 | A kind of Grifola frondosa culture medium prescription and preparation method thereof |
| CN108812070A (en) * | 2018-09-30 | 2018-11-16 | 福州东星生物技术有限公司 | A kind of grifola frondosus culture substrate |
| CN111264343A (en) * | 2020-03-13 | 2020-06-12 | 江苏华绿生物科技股份有限公司 | Culture medium for industrial cultivation of grifola frondosa and preparation method thereof |
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| CN106242856A (en) * | 2016-08-30 | 2016-12-21 | 贵州瑞泰农业科技有限公司 | A kind of Grifola frondosa culture medium prescription and preparation method thereof |
| CN108812070A (en) * | 2018-09-30 | 2018-11-16 | 福州东星生物技术有限公司 | A kind of grifola frondosus culture substrate |
| CN111264343A (en) * | 2020-03-13 | 2020-06-12 | 江苏华绿生物科技股份有限公司 | Culture medium for industrial cultivation of grifola frondosa and preparation method thereof |
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