CN117288975A - Multi-sample synchronous immunochromatography detection method - Google Patents
Multi-sample synchronous immunochromatography detection method Download PDFInfo
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- CN117288975A CN117288975A CN202210680147.4A CN202210680147A CN117288975A CN 117288975 A CN117288975 A CN 117288975A CN 202210680147 A CN202210680147 A CN 202210680147A CN 117288975 A CN117288975 A CN 117288975A
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- 238000001514 detection method Methods 0.000 title claims abstract description 133
- 238000003317 immunochromatography Methods 0.000 title claims abstract description 24
- 230000001360 synchronised effect Effects 0.000 title claims abstract description 17
- 238000012360 testing method Methods 0.000 claims abstract description 73
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 239000003814 drug Substances 0.000 claims abstract description 17
- 238000007789 sealing Methods 0.000 claims description 32
- 238000003556 assay Methods 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 239000002274 desiccant Substances 0.000 claims description 6
- 238000003825 pressing Methods 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 3
- 230000007306 turnover Effects 0.000 abstract description 3
- 230000000670 limiting effect Effects 0.000 description 12
- 230000000694 effects Effects 0.000 description 10
- 239000011324 bead Substances 0.000 description 5
- 238000010586 diagram Methods 0.000 description 5
- 238000009423 ventilation Methods 0.000 description 5
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000001746 injection moulding Methods 0.000 description 3
- 230000007704 transition Effects 0.000 description 3
- 230000009471 action Effects 0.000 description 2
- 230000004323 axial length Effects 0.000 description 2
- 238000004587 chromatography analysis Methods 0.000 description 2
- 230000007423 decrease Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 238000010828 elution Methods 0.000 description 2
- 238000001310 location test Methods 0.000 description 2
- 238000000465 moulding Methods 0.000 description 2
- 239000004033 plastic Substances 0.000 description 2
- 229920003023 plastic Polymers 0.000 description 2
- 238000007790 scraping Methods 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
- G01N35/04—Details of the conveyor system
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/5302—Apparatus specially adapted for immunological test procedures
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
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Abstract
The invention discloses a multi-sample synchronous immunochromatography detection method, which comprises the steps of placing a detection tube after sample addition on a tray, and enabling a tube body of the detection tube to be positioned below a tube cover; or placing the detection tube on a tray, enabling the tube body of the detection tube to be positioned below the tube cover, and then adding samples to the detection tube; turning over the tray to enable the liquid medicament containing the sample to be tested to be in contact with the test strip, and starting timing; and judging the detection results of all the detection tubes after the set detection time is reached. According to the multi-sample synchronous immunochromatography detection method, after sample addition is completed on the detection tubes, before overturning, the tube body is positioned below the tube cover, after overturning the tray, the liquid medicament is contacted with the test strip, and all the detection tubes placed on the tray synchronously perform immunochromatography detection, so that the detection results of all the detection tubes can be read after the detection time is up, and the overtime interpretation condition is avoided; the tray drives the plurality of detection tubes to turn over simultaneously, so that the technical purpose of synchronously carrying out immunochromatography detection on samples is realized.
Description
Technical Field
The invention belongs to the technical field of immunochromatography detection, and particularly relates to a multi-sample synchronous immunochromatography detection method.
Background
The immunochromatography detection technology is to apply antigen-antibody immunological reaction and chromatographic reaction, and to achieve the purpose of rapidly and accurately developing color to detect the object to be detected in the form of dry-sheet test paper. In the traditional immunochromatography detection, single samples are collected one by one, then the samples are processed uniformly, and finally the sample adding detection is carried out one by one. Because the detection starting time of the samples is inconsistent, the result interpretation time cannot be effectively grasped, and overtime interpretation is frequently caused.
Disclosure of Invention
Accordingly, the present invention is directed to a multi-sample simultaneous immunochromatography detection method capable of simultaneously performing immunochromatography detection on a plurality of samples to improve detection efficiency.
In order to achieve the above purpose, the present invention provides the following technical solutions:
a multi-sample synchronous immunochromatography detection method comprises the steps of placing a detection tube after sample addition on a tray, and enabling a tube body of the detection tube to be positioned below a tube cover; or placing the detection tube on a tray, enabling the tube body of the detection tube to be positioned below the tube cover, and then adding samples to the detection tube;
turning over the tray to enable the liquid medicament containing the sample to be tested to be in contact with the test strip, and starting timing;
and judging the detection results of all the detection tubes after the set detection time is reached.
Further, a first cavity and a second cavity are arranged in the tube body, the test strip is placed in the first cavity, the liquid medicament is stored in the second cavity, and the second cavity is sealed by the sealing film;
the method for adding the sample in the detection tube comprises the following steps:
removing the sealing film on the second cavity by taking the pipe cover off the pipe body;
extending at least one sampling swab into the second cavity, and enabling a sample to be tested to be left in the second cavity to be mixed with the liquid medicament;
and (5) covering the tube cover on the tube body to finish sample adding.
Further, a third cavity for communicating the first cavity with the second cavity is formed between the pipe cover and the pipe body.
Further, when a desiccant is placed in the third chamber, the desiccant is removed first after the tube cover is removed from the tube body, and then the sealing film on the second chamber is released.
Further, the top surface is provided with a first opening, and the test strip is placed into the first cavity through the first opening; a fixed plug is arranged in the first opening, a first section of the test strip penetrates through a yielding groove on the fixed plug, and the test strip is fixedly connected with the fixed plug through a first end of the test strip.
Further, the second end of the test strip is fixed by the pressing rib arranged on the side wall of the first cavity.
Further, an air hole for balancing the internal pressure and the external pressure is formed in the first cavity; and ribs for eluting the sample on the swab are arranged on the side wall of the second cavity.
Further, a cover groove with an opening at the bottom surface is arranged in the pipe cover, and a pipe cover bulge protruding downwards is arranged at the bottom of the cover groove so as to reduce the volume of the third cavity.
Further, the device is used for simultaneously detecting at least two reagent tubes.
Further, a plurality of slots for placing the detection tubes are arranged on the tray, and the method for placing the detection tubes after the sample addition on the tray is as follows:
when the slot is matched with the pipe body, the pipe body is inserted into the slot from top to bottom after the sample addition is completed;
when the slot is matched with the pipe cover, the pipe cover is inserted into the slot from bottom to top after the sample addition is completed;
when the slot is integrally matched with the detection tube, the tray is positioned in the vertical direction, the detection tube is embedded into the slot after sample addition is completed, and the tube body of the detection tube is positioned below the tube cover.
Further, when the trays have at least two, stacking the trays together after placing the test tubes after the completion of the loading on the trays, or after placing the test tubes on the trays and the completion of the loading; when the trays are turned over, the stacked trays are turned over synchronously.
The invention has the beneficial effects that:
according to the multi-sample synchronous immunochromatography detection method, after the detection tube is subjected to sample addition, the detection tube is placed on a tray, or the detection tube is placed on the tray and then sample addition is performed; at this time, the tube body is positioned below the tube cover, so that immunochromatography detection is not performed yet; then the tray is turned over to enable the liquid medicament containing the sample to be tested to be in contact with the test strip, all the detection tubes placed on the tray synchronously perform immunochromatography detection, timing is started, after the detection time is reached, the detection results of all the detection tubes can be judged, and the condition of overtime judgment can be avoided; the tray drives the plurality of detection tubes to turn over simultaneously so as to realize the technical purpose of synchronously carrying out immunochromatography detection on a plurality of samples, and the detection efficiency can be effectively improved.
Drawings
In order to make the objects, technical solutions and advantageous effects of the present invention more clear, the present invention provides the following drawings for description:
FIG. 1 is a flow chart of an embodiment of a multi-sample simultaneous immunochromatographic assay method of the present invention;
fig. 2 is a schematic structural diagram of the test tube after being placed on the tray, specifically a schematic structural diagram when the slot is matched with the tube body;
fig. 3 is a schematic structural view of the test tube after being placed on the tray, specifically, a schematic structural view when the socket is matched with the tube cover;
fig. 4 is a schematic structural diagram of the test tube after being placed on the tray, specifically a schematic structural diagram when the slot is integrally matched with the test tube;
FIG. 5 is a schematic view of the structure of two trays stacked together;
FIG. 6 is a schematic structural view of a detection tube according to a first example;
FIG. 7 is an exploded view of the test tube;
FIG. 8 is a schematic structural view of a fixed plug;
FIG. 9 is an upper perspective view of the test tube of the present embodiment;
FIG. 10 is a lower perspective view of the test tube of the present embodiment;
FIG. 11 is a schematic view of the structure of a pipe body;
FIG. 12 is a schematic view of the structure of the tube cap;
FIG. 13 is a perspective view of a tube cap;
FIG. 14 is a perspective view of a tube body;
FIG. 15 is a schematic diagram of a test strip;
FIG. 16 is a schematic view showing the structure of a detection tube according to a second example;
FIG. 17 is an exploded view of the test tube;
fig. 18 is a schematic structural view of the tube cap.
Reference numerals illustrate:
1-a test strip; 2-a first end; 3-NC film; 4-a second end; 5-a tray; 6-slots;
10-a tube body; 10 a-upper part of the tube body; 10 b-lower part of the tube body; 11-a first cavity; 12-a first opening; 13-fixing the plug; 14-a yielding groove; 15-pressing the ribs; 16-guiding inclined plane; 17-results observation area; 18-ventilation holes; 19-a second cavity; 19 a-a lower chamber; 19 b-transition; 20-a second opening; 21-ribs; 22-closing-in section; 23-injection molding wave point; 24-limiting ribs; 25-sealing ribs; 26-positioning protrusions; 27-reducing section;
30-tube cap; 31-cover groove; 31 a-upper side wall portion; 31 b-lower side wall portion; 32-covering the mouth; 33-a third cavity; 34-sealing the groove; 35-positioning grooves; 36-tube cap protrusions; 37-sealing ribs.
Detailed Description
The present invention will be further described with reference to the accompanying drawings and specific examples, which are not intended to limit the invention, so that those skilled in the art may better understand the invention and practice it.
The detection tube suitable for the multi-sample synchronous immunochromatography detection method comprises a tube body 10 and a tube cover 30, wherein a first cavity 11 and a second cavity 19 are arranged in the tube body 10, a test strip 1 is placed in the first cavity 11, a liquid medicament is stored in the second cavity 19, and a sealing film is arranged on the second cavity 19 to seal the second cavity. After the tube cover 30 is covered on the tube body 10, a third cavity 33 for communicating the first cavity 11 and the second cavity 19 is formed between the tube cover 30 and the tube body 10.
As shown in FIG. 1, the multi-sample synchronous immunochromatography detection method of the present embodiment includes two steps of sample addition and detection. The method for adding the sample in the detection tube comprises the following steps:
removing the tube cover 30 from the tube body 10, and releasing the sealing film on the second cavity 19; specifically, when the desiccant is placed in the third cavity 33, the desiccant should be removed first and then the sealing film on the second cavity 19 should be released after the tube cover 30 is removed from the tube body 10;
extending at least one sampling swab into the second cavity 19 and leaving the sample to be tested in the second cavity 19 for mixing with the liquid medicament; the tube cover 30 is covered on the tube body 10 to finish sample application.
The method for synchronously detecting the multiple samples comprises the following steps:
placing the detection tube after sample addition on a tray 5, and positioning a tube body 10 of the detection tube below a tube cover 30; or the detection tube is firstly placed on the tray 5, the tube body 10 of the detection tube is positioned below the tube cover 30, and then the detection tube is subjected to sample adding;
turning over the tray 5 to enable the liquid medicament containing the sample to be tested to be in contact with the test strip, and starting timing;
and judging the detection results of all the detection tubes after the set detection time is reached.
Specifically, a plurality of slots 6 for placing detection tubes are arranged on the tray 5, and when the detection tubes are placed on the tray 5 after sample addition is completed, the slots 6 and the detection tubes are provided with various matching modes, for example, the following steps:
as shown in fig. 2, when the slot 6 is matched with the pipe body 10, after the sample is added, the pipe body 10 is inserted into the slot 6 from top to bottom;
as shown in fig. 3, when the slot 6 is matched with the tube cover 30, after the sample is added, the tube cover 30 is inserted into the slot 6 from bottom to top;
as shown in fig. 4, when the slot 6 is integrally matched with the test tube, the tray 5 is located in the vertical direction, the test tube is inserted into the slot 6 after the sample is applied, and the tube body 10 of the test tube is located below the tube cover 30.
The three modes can realize the technical purposes that the detection tube after the sample addition is placed on the tray 5 and the tray 5 is turned over, and the detection tube is turned over synchronously along with the tray 5. Of course, the matching relationship between the detection tube and the tray may be implemented in other various manners, as long as it is satisfied that the detection tube can be turned synchronously with the tray when the tray 5 is turned.
In addition, when the tray 5 has at least two, after the test tubes after the completion of the sample addition are placed on the tray 5, or after the test tubes are placed on the tray 5 and the sample addition is completed, the trays 5 are stacked together; when the trays 5 are turned over, the stacked trays 5 are turned over synchronously, so that the detection tubes placed in the plurality of trays 5 are turned over synchronously and synchronous detection is achieved, as shown in fig. 5. Specifically, a stacking structure which is convenient for stacking is arranged on the tray 5 at the moment, and the stacking structure can be realized in various existing modes without being tired.
In the multi-sample synchronous immunochromatography detection method of the embodiment, after the sample is added to the detection tube, the detection tube is placed on the tray 5, or the detection tube is placed on the tray 5 and then the sample is added; at this time, since the tube body 10 is positioned below the tube cover 30, no immunochromatographic detection has been performed yet; then the tray is turned over to enable the liquid medicament containing the sample to be tested to be in contact with the test strip, all the detection tubes placed on the tray 5 synchronously perform immunochromatography detection, timing is started, after the detection time is reached, the detection results of all the detection tubes can be judged and read, and the condition of overtime judgment can be avoided; the tray 5 drives the plurality of detection tubes to turn over simultaneously so as to realize the technical purpose of synchronously carrying out immunochromatography detection on a plurality of samples, namely, the multi-sample synchronous immunochromatography detection method of the embodiment can be used for simultaneously detecting at least two reagent tubes, and can effectively improve the detection efficiency.
The following describes specific embodiments of a detection tube according to the multi-sample simultaneous immunochromatographic detection method of the present embodiment with two specific examples.
The embodiment of the detection tube of the first example is as follows.
As shown in fig. 6-7, the detection tube comprises a tube body 10 and a tube cover 30, at least one first cavity 11 for placing the test strip 1 is arranged in the tube body 10, a first opening 12 for the test strip 1 to pass through is arranged on the top surface of the first cavity 11, a fixed plug 13 is arranged on the first opening 12, a yielding groove 14 matched with the first end 2 of the test strip 1 is arranged on the fixed plug 13 as shown in fig. 8, and the first end of the test strip 1 passes through the yielding groove 14 arranged on the fixed plug 13 so as to fix the first end of the test strip. The test strip 1 is placed through setting up first cavity 11 to set up fixed stopper 13 on the first opening 12 of first cavity 11, fixed stopper 13 not only can play the effect of the first end of location test strip 1, in the detection tube inversion in-process moreover, can prevent effectively that the liquid reagent that splashes from entering into in the first cavity 11, and then avoid the liquid reagent pollution test strip that splashes, can effectively improve the success rate of chromatography detection. The tube body 10 is internally provided with a first cavity 11, when the tube is used, one test strip 1 can be placed in the first cavity 11 to realize the detection of a single item, and at least two test strips 1 can be placed in the first cavity 11 at the same time to realize the synchronous detection of two or more items. Of course, in other embodiments, two or more first cavities 11 may be disposed in the tube 10, and at this time, the test strips 1 may be selectively disposed in different first cavities 11 to realize synchronous detection of multiple items.
Further, as shown in fig. 9, a result observation area 17 is disposed on a side wall of the first cavity 11, and a highlight convex surface is disposed in the result observation area 17, so as to be convenient for reading the detection result.
Further, as shown in fig. 10, ventilation holes 18 are provided in the first chamber 11 to balance the internal and external air pressures. Specifically, after the detection tube is inverted, the test strip 1 can be placed in the same pressure environment as the outside, and the chromatographic detection effect can be effectively improved. The ventilation holes 18 are provided on the bottom surface of the first chamber 11. In the production process, the defect that the rubber is punched and bent to cause product perforation and the like can be avoided, and the requirement of die demolding can be met.
Further, as shown in fig. 11, a closing-in section 22 is provided at the bottom of the second cavity 19, the inside diameter of the closing-in section 22 gradually decreases along the top-to-bottom direction, and an injection molding wave point 23 is provided at the bottom of the closing-in section 22. Through setting up the binding off section in the bottom of second cavity to set up the ripples point of moulding plastics in the bottom of binding off section, so, can make the bottom of second cavity thick, in the production process, with the assurance mould advance gluey.
Further, a pressing rib 15 for positioning the second end 4 of the test strip 1 is arranged on the side wall of the first cavity 11, and the second end of the test strip 1 is fixedly positioned through the pressing rib 15 arranged on the side wall of the first cavity 11. Through setting up the clamp 15, can fix a position the second end 4 of test paper strip 1, prevent that test paper strip 1 from rocking to make test paper strip 1 paste on the lateral wall of first cavity 11 in order to improve the chromatographic effect, as shown in fig. 11. Preferably, a guiding inclined plane 16 for guiding the test strip 1 is arranged at the top of the rib 15, so that the test strip 1 can be easily placed at a designated position of the first cavity 11 under the guiding action of the guiding inclined plane 16.
Further, as shown in fig. 11, a second cavity 19 for storing a liquid medicine is provided in the tube body 10, and a second opening 20 is provided on the top surface of the second cavity 19. The rib 21 which is convenient for eluting the sample on the swab is arranged on the side wall of the second cavity 19, and the sample on the swab is convenient to elute by utilizing the scraping effect of the rib 21 on the swab, so that the sample elution rate is improved.
As shown in fig. 11, a limiting rib 24 for limiting a pipe cover 30 is arranged on the outer wall of the pipe body 10, the pipe body is divided into a pipe body upper part 10a and a pipe body lower part 10b by the limiting rib 24, and the pipe cover 30 covers the pipe body upper part 10 a; the axial length of the upper tube body portion 10a is smaller than the depth of the cover groove 31, so that after the tube cover 30 is covered on the upper tube body portion 10a, a gap is formed between the groove bottom of the cover groove 31 and the top surface of the tube body 10 under the limiting action of the limiting ribs 24, thereby forming a third cavity 33.
Further, as shown in fig. 12 to 13, a lid groove 31 having an opening at the bottom surface is provided in the tube lid 30, a notch of the lid groove 31 forms a lid opening 32, the tube lid 30 is covered on the upper end of the tube body 10, and a third cavity 33 for communicating the first cavity 11 and the second cavity 19 is formed between the tube lid 30 and the tube body 10. The bottom of the cover groove 31 is provided with a pipe cover protrusion 36 protruding downward to reduce the volume of the third cavity 33, so as to reduce the requirement for the use amount of the liquid medicine.
Further, a sealing structure is provided between the pipe body 10 and the pipe cover 30. The sealing structure comprises a sealing rib 25 and a sealing groove 34 which are matched with each other. Specifically, the sealing structure may be such that the sealing bead 25 is provided on the outer wall of the upper portion 10a of the pipe body, as shown in fig. 14. A seal groove 34 is provided on the side wall of the cover groove 31 as shown in fig. 12. The sealing structure may be that a sealing groove 34 is provided on the outer wall of the upper portion 10a of the pipe body, and a sealing bead 25 is provided on the side wall of the cover groove 31. The sealing bead 25 is provided on the outer wall of the upper body portion 10a, and the sealing groove 34 is provided on the side wall of the cover groove 31.
Further, a positioning structure is provided between the pipe body 10 and the pipe cover 30. The positioning structure comprises positioning protrusions 26 and positioning grooves 35 which are arranged in one-to-one correspondence with the positioning protrusions 26. Specifically, the positioning structure may be such that the positioning protrusion 26 is provided on the outer wall of the upper tube body portion 10a, as shown in fig. 14. The positioning groove 35 is provided on the side wall of the cover groove 31 as shown in fig. 12. The positioning structure may be such that the positioning groove 35 is provided on the outer wall of the upper tube body portion 10a and the positioning projection 26 is provided on the side wall of the cover groove 31. The positioning projection 26 is provided on the outer wall of the tube body upper portion 10a, and the positioning groove 35 is provided on the side wall of the cover groove 31.
As shown in fig. 11 to 12, the side wall of the cover groove 31 includes an upper side wall section 31a at an upper portion and a lower side wall section 31b at a lower portion, the geometry of the lower side wall section 31b is larger than that of the upper side wall section 31a, the positioning projection 26 or the positioning groove 35 is provided on the lower side wall section 31b, and the sealing bead 25 or the sealing groove 34 is provided on the upper side wall section 31 a. In this embodiment, the positioning boss 26 is provided on the side wall lower section 31b, and the seal bead 25 is provided on the side wall upper section 31 a.
The embodiment of the detection tube of the second example is as follows.
As shown in fig. 16, the detection tube includes a tube body 10 and a tube cover 30, at least one first cavity 11 for placing the test strip 1 is provided in the tube body 10, a first opening 12 for the test strip 1 to pass through is provided on the top surface of the first cavity 11, a fixing plug 13 (not shown in the figure) is mounted on the first opening 12, and a yielding groove 14 matched with the first end 2 of the test strip 1 is provided on the fixing plug 13. The test tube is through setting up first cavity 11 and placing test strip 1 to set up fixed stopper 13 on the first opening 12 of first cavity 11, fixed stopper 13 not only can play the effect of the first end of location test strip 1, in the test tube inversion in-process moreover, can prevent effectively that the liquid reagent that splashes from entering into in the first cavity 11, and then avoid the NC membrane 3 of the liquid reagent pollution test strip that splashes, can effectively improve the success rate that the chromatography detected. The tube body 10 is internally provided with a first cavity 11, when the tube is used, one test strip 1 can be placed in the first cavity 11 to realize the detection of a single item, and at least two test strips 1 can be placed in the first cavity 11 at the same time to realize the synchronous detection of two or more items. Of course, in other embodiments, two or more first cavities 11 may be disposed in the tube 10, and at this time, a test strip may be selectively disposed in different first cavities 11 to realize synchronous detection of multiple items.
Further, as shown in fig. 16, a rib 15 for positioning the second end 4 of the test strip 1 is provided on the side wall of the first cavity 11. Through setting up the clamp 15, can fix a position the second end 4 of test paper strip 1, prevent that test paper strip 1 from rocking to make test paper strip 1 paste on the lateral wall of first cavity 11 in order to heighten the chromatographic effect. Preferably, a guiding inclined plane 16 for guiding the test strip 1 is arranged at the top of the rib 15, so that the test strip 1 can be easily placed at a designated position of the first cavity 11 under the guiding action of the guiding inclined plane 16.
Further, a result observation area 17 is arranged on the side wall of the first cavity 11, and a highlight convex surface is arranged in the result observation area 17, so that the detection result can be conveniently read.
Further, the ventilation holes 18 are formed in the first cavity 11, and after the detection tube is inverted, the test strip 1 can be placed in the same pressure environment as the outside, so that the chromatographic detection effect can be effectively improved. The ventilation holes 18 are provided on the bottom surface of the first chamber 11. In the production process, the defect that the rubber is punched and bent to cause product perforation and the like can be avoided, and the requirement of die demolding can be met.
Further, a second cavity 19 for storing a liquid medicine is provided in the tube body 10, and a second opening 20 is provided on the top surface of the second cavity 19. The rib 21 which is convenient for eluting the sample on the swab is arranged on the side wall of the second cavity 19, and the sample on the swab is convenient to elute by utilizing the scraping effect of the rib 21 on the swab, so that the sample elution rate is improved.
Further, the second cavity 19 includes a lower cavity 19a located at a lower portion and a transition section 19b located at an upper portion, the second opening 20 is square, and an inner wall of the lower cavity 19a is cylindrical; the transition section 19b serves to smoothly blend between the second opening 20 and the lower chamber 19 a. Through setting up the changeover portion and make smooth transition between second opening and the lower chamber, can prevent that liquid from hanging in second opening position department, be convenient for encapsulate.
Further, a closing-in section 22 is provided at the bottom of the second cavity 19, the inside diameter of the closing-in section 22 gradually decreases along the direction from top to bottom, and an injection molding wave point 23 is provided at the bottom of the closing-in section 22. Through setting up the binding off section in the bottom of second cavity to set up the ripples point of moulding plastics in the bottom of binding off section, so, can make the bottom of second cavity thick, in the production process, with the assurance mould advance gluey.
Further, as shown in fig. 18, a lid groove 31 having an opening at the bottom surface is provided in the tube lid 30, a lid opening 32 is formed in the notch of the lid groove 31, the tube lid 30 is covered on the upper end of the tube body 10, and a third cavity 33 for communicating the first cavity 11 and the second cavity 19 is formed between the tube lid 30 and the tube body 10. The bottom of the cover groove 31 is provided with a pipe cover protrusion 36 protruding downward to reduce the volume of the third cavity 33, so as to reduce the requirement for the use amount of the liquid medicine.
As shown in fig. 17, a limiting rib 24 for limiting a pipe cover 30 is arranged on the outer wall of the pipe body 10, the pipe body is divided into a pipe body upper part 10a and a pipe body lower part 10b by the limiting rib 24, and the pipe cover 30 covers the pipe body upper part 10 a; the axial length of the upper tube body portion 10a is smaller than the depth of the cover groove 31, so that after the tube cover 30 is covered on the upper tube body portion 10a, a gap is formed between the groove bottom of the cover groove 31 and the top surface of the tube body 10 under the limiting action of the limiting ribs 24, thereby forming a third cavity 33.
Further, a sealing structure is arranged between the pipe body 10 and the pipe cover 30, and the sealing structure adopts a sealing rib 37 arranged between the pipe cover 30 and the limiting convex rib 24. Specifically, the sealing rib 37 may be disposed on the bottom surface of the tube cover 30, and the sealing rib may be disposed on the upper side surface of the limit rib 24. Sealing ribs 37 may be provided on the bottom surface of the tube cover 30. In order to ensure enough binding force between the pipe body 10 and the pipe cover 30 to ensure the sealing effect, a reducing section 27 for interference fit with the pipe cover 30 is arranged at the position of the upper part 10a of the pipe body close to the limiting convex rib 24; the cross sections of any two planes parallel to each other and perpendicular to the axis of the pipe body are similar in pattern, and the geometric dimension of the cross section of the plane at the lower part is larger than that of the cross section of the plane at the upper part, so that the bonding force between the pipe cover 30 and the pipe body 10 can be ensured.
Further, a positioning structure is provided between the pipe body 10 and the pipe cover 30. The positioning structure comprises positioning protrusions 26 and positioning grooves 35 which are arranged in one-to-one correspondence with the positioning protrusions 26. Specifically, the positioning structure may be that the positioning protrusion 26 is disposed on the outer wall of the upper portion 10a of the pipe body, and the positioning groove 35 is disposed on the side wall of the cover groove 31; the positioning structure may be such that the positioning groove 35 is provided on the outer wall of the upper tube body portion 10a and the positioning projection 26 is provided on the side wall of the cover groove 31. The positioning projection 26 is provided on the outer wall of the tube body upper portion 10a, and the positioning groove 35 is provided on the side wall of the cover groove 31.
The above-described embodiments are merely preferred embodiments for fully explaining the present invention, and the scope of the present invention is not limited thereto. Equivalent substitutions and modifications will occur to those skilled in the art based on the present invention, and are intended to be within the scope of the present invention. The protection scope of the invention is subject to the claims.
Claims (10)
1. A multi-sample synchronous immunochromatography detection method is characterized in that:
placing the detection tube after the sample addition on a tray, and enabling the tube body of the detection tube to be positioned below the tube cover; or placing the detection tube on a tray, enabling the tube body of the detection tube to be positioned below the tube cover, and then adding samples to the detection tube;
turning over the tray to enable the liquid medicament containing the sample to be tested to be in contact with the test strip, and starting timing;
and judging the detection results of all the detection tubes after the set detection time is reached.
2. The multiple sample simultaneous immunochromatographic assay method according to claim 1, wherein: a first cavity and a second cavity are arranged in the tube body, the test strip is placed in the first cavity, the liquid medicament is stored in the second cavity, and the second cavity is sealed by a sealing film;
the method for adding the sample in the detection tube comprises the following steps:
removing the sealing film on the second cavity by taking the pipe cover off the pipe body;
extending at least one sampling swab into the second cavity, and enabling a sample to be tested to be left in the second cavity to be mixed with the liquid medicament;
and (5) covering the tube cover on the tube body to finish sample adding.
3. The multiple sample simultaneous immunochromatographic assay method according to claim 2, wherein: and a third cavity for communicating the first cavity with the second cavity is formed between the pipe cover and the pipe body.
4. The multiple sample simultaneous immunochromatographic assay method according to claim 3, wherein: when the drying agent is placed in the third cavity, the drying agent is removed first after the pipe cover is removed from the pipe body, and then the sealing film on the second cavity is released.
5. The multiple sample simultaneous immunochromatographic assay method according to claim 2, wherein: a first opening is arranged on the top surface of the first cavity, and the test strip is placed into the first cavity through the first opening; a fixed plug is arranged in the first opening, a first section of the test strip penetrates through a yielding groove on the fixed plug, and the test strip is fixedly connected with the fixed plug through a first end of the test strip;
the second end of the test strip is fixed by a pressing rib arranged on the side wall of the first cavity.
6. The multiple sample simultaneous immunochromatographic assay method according to claim 2, wherein: an air hole for balancing the internal pressure and the external pressure is formed in the first cavity; and ribs for eluting the sample on the swab are arranged on the side wall of the second cavity.
7. The multiple sample simultaneous immunochromatographic assay method according to claim 3, wherein: a cover groove with an opening at the bottom surface is arranged in the pipe cover, and a pipe cover bulge which protrudes downwards is arranged at the bottom of the cover groove so as to reduce the volume of the third cavity.
8. The multiple sample simultaneous immunochromatographic assay method according to any one of claims 1 to 7, which is characterized in that: for simultaneous detection of at least two reagent tubes.
9. The multiple sample simultaneous immunochromatographic assay method according to any one of claims 1 to 7, which is characterized in that: the tray is provided with a plurality of slots for placing the detection tubes, and the method for placing the detection tubes after the sample addition on the tray is as follows:
when the slot is matched with the pipe body, the pipe body is inserted into the slot from top to bottom after the sample addition is completed;
when the slot is matched with the pipe cover, the pipe cover is inserted into the slot from bottom to top after the sample addition is completed;
when the slot is matched with the pipe cover, the pipe cover is inserted into the slot from bottom to top after the sample addition is completed;
when the slot is integrally matched with the detection tube, the tray is positioned in the vertical direction, the detection tube is embedded into the slot after sample addition is completed, and the tube body of the detection tube is positioned below the tube cover.
10. The multiple sample simultaneous immunochromatographic assay method according to any one of claims 1 to 7, which is characterized in that: when the trays are provided with at least two, stacking the trays together after placing the detection pipes after the sample addition on the trays or after placing the detection pipes on the trays and completing the sample addition; when the trays are turned over, the stacked trays are turned over synchronously.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210680147.4A CN117288975A (en) | 2022-06-16 | 2022-06-16 | Multi-sample synchronous immunochromatography detection method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210680147.4A CN117288975A (en) | 2022-06-16 | 2022-06-16 | Multi-sample synchronous immunochromatography detection method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117288975A true CN117288975A (en) | 2023-12-26 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210680147.4A Pending CN117288975A (en) | 2022-06-16 | 2022-06-16 | Multi-sample synchronous immunochromatography detection method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117288975A (en) |
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2022
- 2022-06-16 CN CN202210680147.4A patent/CN117288975A/en active Pending
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