CN105938066A - Liquid mixing device and mixing method - Google Patents
Liquid mixing device and mixing method Download PDFInfo
- Publication number
- CN105938066A CN105938066A CN201610525481.7A CN201610525481A CN105938066A CN 105938066 A CN105938066 A CN 105938066A CN 201610525481 A CN201610525481 A CN 201610525481A CN 105938066 A CN105938066 A CN 105938066A
- Authority
- CN
- China
- Prior art keywords
- reagent
- chamber
- pressing plate
- sealing member
- reagent container
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D11/00—Control of flow ratio
- G05D11/02—Controlling ratio of two or more flows of fluid or fluent material
- G05D11/03—Controlling ratio of two or more flows of fluid or fluent material without auxiliary power
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/38—Diluting, dispersing or mixing samples
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/523—Containers specially adapted for storing or dispensing a reagent with means for closing or opening
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/52—Containers specially adapted for storing or dispensing a reagent
- B01L3/527—Containers specially adapted for storing or dispensing a reagent for a plurality of reagents
-
- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D11/00—Control of flow ratio
- G05D11/001—Control of flow ratio with discontinuous action
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0689—Sealing
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1002—Reagent dispensers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
- G01N35/1009—Characterised by arrangements for controlling the aspiration or dispense of liquids
- G01N35/1016—Control of the volume dispensed or introduced
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- General Physics & Mathematics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Automation & Control Theory (AREA)
- Medicinal Chemistry (AREA)
- Clinical Laboratory Science (AREA)
- Engineering & Computer Science (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Package Specialized In Special Use (AREA)
- Life Sciences & Earth Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
Abstract
The invention discloses a liquid mixing device, which comprises a reagent accommodation cavity, a mixing cavity, and a channel between the accommodation cavity and the mixing cavity. The lateral wall of the channel is provided with an injection port. A reagent container is arranged in the reagent accommodation cavity. The top and the bottom of the reagent container are opened and respectively provided with a first sealing member and a second sealing member. The first sealing member comprises a press plate arranged on the top opening of the reagent container and a cover plate arranged on the top of the press plate. The press plate is provided with a press plate through hole. The press plate through hole is opposite to the top opening of the reagent container. The cover plate and the second sealing member are detachable. The invention also discloses a mixing method of the liquid mixing device. The provided liquid mixing device has a good sealing property, the cross contamination of reagents is effectively avoided by setting the press plate through hole, and moreover, the using amount of reagents can be precisely controlled.
Description
Technical field
The present invention relates to apparatus for combining liquids and mixed method thereof.
Background technology
In biochemical analysis, it usually needs micro-example mixes with different classes of trace reagent, then passes through light
The means such as electro-detection are gone detection, are analyzed.But the reagent added is mostly individually placed with big packaged form, adding
Added-time needs operator to use special dosage instrument to join in mixing pit quantitatively, exactly.Reagent is many to drip
Vial-type contains, and the interference of in use anthropic factor is relatively big, affects the accuracy of result, and generally requires and join
Put extra adnexa, field quick detection is brought inconvenience.
Summary of the invention
Goal of the invention: liquid that it is an object of the invention to provide a kind of simple in construction, that can accurately control reagent dosage
Body mixing arrangement and mixed method thereof.
Technical scheme: for reaching this purpose, the present invention by the following technical solutions:
Apparatus for combining liquids of the present invention, accommodates chamber, hybrid chamber and reagent including reagent and accommodates chamber and mix
Passage between chamber, the sidewall of passage is provided with adding mouth, and reagent accommodates intracavity portion and is provided with reagent container, and reagent holds
The equal opening in device top and bottom, the top in reagent receiving chamber and bottom are respectively equipped with the first sealing member and second and seal
Part, the first sealing member includes being located at the pressing plate of reagent container top open part and being located at the cover plate at pressing plate top, pressing plate
Being provided with pressing plate through hole, pressing plate through hole is connected with the open top of reagent container, cover plate and the second sealing member
Dismounting.
Sealing during in order to place when improving reagent container top horizontal, is provided with the first elastomeric material bottom described pressing plate
Layer, in order to improve sealing during reagent container top inflatable, also is provided with the first elastic material inside described pressing plate through hole
The bed of material.
In order to improve the sealing bottom reagent container, described second sealing member is provided with the second elastomeric layer, the
The bottom opening of reagent container is sealed by two elastomeric layers.
Sealing during in order to place when improving reagent container top horizontal, is provided with projection, cover plate lid bottom described cover plate
Time on pressing plate, in protruding embedding pressing plate through hole.
In order to improve sealing during reagent container top inflatable, the cross-sectional area of described pressing plate through hole holds less than reagent
The open-topped cross-sectional area of device.
Drip to prevent from being formed to hang at reagent container bottom opening during inflation in reagent container, improve examination
The accuracy of agent injection rate, the inwall of described reagent container bottom is provided with the boss extended internally.
In order to reduce the volume of whole apparatus for combining liquids, described reagent container top accommodates top of chamber less than reagent,
The thickness of described pressing plate accommodates the distance between top of chamber equal to reagent container top to reagent.
For the ease of hand-held apparatus for combining liquids, it is hand-held that the sidewall in described reagent receiving chamber is provided with reagent receiving chamber
Portion, reagent accommodates Handheld Division, chamber and is provided with the first groove.
For the ease of dismantling the second sealing member, described second sealing member is provided with the second sealing member Handheld Division, and second is close
Sealing Handheld Division is provided with the second groove.
Liquid mixing method of the present invention, comprises the following steps:
S1: reagent accommodates in chamber and encapsulates reagent in advance, removes cover plate, extracts the second sealing member out, by adding mouth to
Sample is added in hybrid chamber;
S2: by pressing plate through hole to reagent container inner inflatable, the reagent in reagent container is mixed via passage press-in
Close in chamber, thus reagent is mixed with sample.
Beneficial effect: compared with prior art, the present invention has a following beneficial effect:
1) the reagent receiving chamber storing reagent is integrated in a device with the hybrid chamber carrying out biochemical reaction by the present invention
In, it is not necessary to store reagent by other devices, simplify the structure of device, reduce cost;
2) present invention has multiple reagent container, it is possible to achieve plurality of reagents simultaneously or sequentially joins hybrid chamber
In;
3) pressing plate of the present invention is provided with pressing plate through hole, it is possible to by inflating in pressing plate through hole, by reagent container
In reagent press-in hybrid chamber in, and the mode of malleation ensure that the accuracy of reagent dosage;
4) setting of the cover plate of the present invention, improves reagent and accommodates the sealing in chamber, effectively achieve reagent
Seal up for safekeeping, the setting of pressing plate through hole, effectively prevent because of be heated or external pressure change and cause reagent to be pressed into
In hybrid chamber, effectively prevent the cross-contamination between reagent, it is achieved that the accurate control to reagent dosage.
Accompanying drawing explanation
Fig. 1 is the decomposition view of the apparatus for combining liquids of embodiments of the invention 1;
Fig. 2 is the sectional view of the apparatus for combining liquids of embodiments of the invention 1;
Fig. 3 is the flow chart of the liquid mixing method of embodiments of the invention 1.
Detailed description of the invention
With detailed description of the invention, technical scheme is further introduced below in conjunction with the accompanying drawings.
Embodiment 1:
Present embodiment discloses a kind of apparatus for combining liquids, as it is shown in figure 1, include that reagent accommodates chamber 91 and mixing
Chamber 61, reagent accommodates by being connected in contraction-like passage 51 from top to bottom between chamber 91 and hybrid chamber 61, logical
The equal opening of top and bottom in road 51, the sidewall of passage 51 is provided with adding mouth 52, it is simple in hybrid chamber 61
Add sample.Reagent receiving chamber 91 sidewall is provided with is easy to hand-held reagent receiving Handheld Division, chamber 32, and reagent accommodates
The outer surface of Handheld Division, chamber 32 is provided with at least two anti-skidding first projections or the first groove 33, to increase hands
Frictional force when holding.Reagent accommodates inside, chamber 91 and is provided with four from top to bottom in contraction-like reagent container 81, as
Shown in Fig. 2, the equal opening in reagent container 81 top and bottom, in order to avoid reagent is at reagent container 81 bottom opening
Place is formed to hang drips, and the inwall of reagent container 81 bottom is provided with the boss extended internally, and boss is along reagent container
81 inwalls are circumferentially arranged.Reagent container 81 top accommodates top, chamber 91 less than reagent, and reagent container 81 top sets
Having the first sealing member, the first sealing member to include pressing plate 21 and cover plate 11, wherein, reagent container is located at by pressing plate 21
81 tops, the thickness of pressing plate 21 accommodates the distance between top, chamber 91 equal to reagent container 81 top to reagent,
Pressing plate 21 is provided with four pressing plate through holes 23, and pressing plate through hole 23 is connected with the open top of reagent container 81,
Bottom pressing plate 21 and being equipped with the first elastomeric layer 22 inside pressing plate through hole 23, the first elastomeric layer 22 is
Silica gel, has, in the middle of the first elastomeric layer 22 inside pressing plate through hole 23, the elastic through hole 221 run through, elastic
The cross-sectional area of through hole 221 is less than the open-topped cross-sectional area of reagent container 81.Cover plate 11 is covered on pressing plate 21
Top, is provided with four downward projections 12 bottom cover plate 11, protruding 12 are embedded in pressing plate through hole 23.Four examinations
Total cross-sectional area of agent container 81 bottom opening is less than the open-topped cross-sectional area of passage 51, in order to reagent can be from
Reagent container 81 bottom opening is flowed in hybrid chamber 61 via passage 51, without being splashed to outside hybrid chamber 61
Face.It is provided with the second sealing member bottom reagent container 81, in passage 51, is provided with the supporting part supporting the second sealing member,
The second sealing member is made to be partially disposed at passage 51 top.The surface of the second sealing member faces reagent container 81
The position of bottom opening is provided with the second elastomeric layer, and the second elastomeric layer is also silica gel such that it is able to by reagent
Container 81 bottom opening seals.On second sealing member, the one end away from the second elastomeric layer is the second sealing member hands
Holding portion 41, it is protruding that the upper surface of the second sealing member Handheld Division 41 and/or lower surface are provided with at least two anti-skidding second
Or the second groove 43.
Additionally, the present embodiment has a biggest advantage, it is simply that if the pressure that reagent accommodates within chamber 91 is more than
Reagent accommodates the pressure outside chamber 91, then take down cover plate 11, and reagent is accommodated chamber 91 with extraneous by pressing plate through hole 23
Connection is got up so that reagent accommodates the pressure balance inside and outside chamber 91, accordingly even when extract the second sealing member out, the most not
The problem that reagent can be occurred to be pressed in hybrid chamber 61, effectively prevent the cross-contamination between reagent, it is achieved that
Accurate control to reagent dosage.
The present embodiment also discloses a kind of method using this apparatus for combining liquids to carry out liquid mixing, as it is shown on figure 3,
Comprise the following steps:
S1: reagent encapsulates reagent in accommodating chamber 91 in advance, removes cover plate 11, extracts the second sealing member out, by adding
Sample mouth 52 adds sample in hybrid chamber 61;
S2: coordinated with pressing plate through hole 23 by the end of gas tube, to reagent container 81 inner inflatable, holds reagent
Reagent in device 81 is pressed in hybrid chamber 61 via passage 51, thus is mixed with sample by reagent.
Embodiment 2:
Present embodiment discloses a kind of apparatus for combining liquids, accommodate chamber and hybrid chamber including reagent, hybrid chamber uses suitable
In the material of detection light transmission, reagent accommodates between chamber and hybrid chamber by from top to bottom in contraction-like passage phase
Even, the equal opening of top and bottom of passage, the sidewall of passage is provided with adding mouth, it is simple to add sample in hybrid chamber
This.Reagent accommodates intracavity portion and is provided with four reagent containers, the equal opening in reagent container top and bottom, reagent container top
Angle between line and the vertical direction at opening center, portion and reagent container bottom opening center is more than zero, four examinations
Line between adjacent two reagent container top opening center, adjacent two reagent container bottom openings in agent container
Off-line between center, the line between same reagent container top opening center and bottom opening center surround inversion
Terrace with edge.Drip in order to avoid reagent forms extension at reagent container bottom opening, the inwall of reagent container bottom sets
Having the boss extended internally, boss is circumferentially arranged along reagent container inwall.Reagent container top is higher than reagent container
And reagent accommodates the joint face between chamber enclosure.Reagent container top is provided with the first sealing member, and the first sealing member includes
Pressing plate and cover plate, wherein, reagent container top is located at by pressing plate, and pressing plate is provided with four pressing plate through holes, pressing plate through hole
It is connected with the open top of reagent container, bottom pressing plate and is equipped with the first elastomeric layer inside pressing plate through hole,
First elastomeric layer is stuck in the outer rim at reagent container top, and the first elastomeric layer is silica gel.Cover plate is covered on pressure
Plate top, is provided with four downward projections bottom cover plate, projection is embedded in pressing plate through hole.It is provided with bottom reagent container
Second sealing member, is provided with the supporting part supporting the second sealing member so that the second sealing member is partially disposed in passage
Channel roof.The surface of the second sealing member faces the position of reagent container bottom opening and is provided with the second elastomeric material
Layer, the second elastomeric layer is also silica gel such that it is able to sealed by reagent container bottom opening.On second sealing member
One end away from the second elastomeric layer is the second sealing member Handheld Division, the upper surface of the second sealing member Handheld Division and/
Or lower surface is provided with at least two antiskid grooves being perpendicular to draw direction.
Additionally, the present embodiment has a biggest advantage, it is simply that if reagent accommodates the pressure in intracavity portion more than reagent
Accommodating the pressure outside chamber, then take down cover plate, reagent is accommodated chamber and is in communication with the outside by pressing plate through hole so that examination
Agent accommodates the pressure balance outside intracavity, accordingly even when extract the second sealing member out, also reagent will not be occurred to be pressed into mixed
Close the problem in chamber, effectively prevent the cross-contamination between reagent, it is achieved that the accurate control to reagent dosage.
Claims (10)
1. apparatus for combining liquids, it is characterised in that: include that reagent accommodates chamber (91), hybrid chamber (61) and examination
Agent accommodates the passage (51) between chamber (91) and hybrid chamber (61), and the sidewall of passage (51) is provided with sample-adding
Mouthful (52), reagent accommodates the internal multiple reagent container (81) that is provided with in chamber (91), reagent container (81) top and
Bottom all openings, reagent accommodates the top in chamber (91) and bottom is respectively equipped with the first sealing member and the second sealing member,
First sealing member includes the pressing plate (21) being located at reagent container (81) top open part and is located at pressing plate (21) top
The cover plate (11) in portion, pressing plate (21) is provided with pressing plate through hole (23), pressing plate through hole (23) and reagent container (81)
Open top be connected, cover plate (11) and the second sealing member are the most detachable.
Apparatus for combining liquids the most according to claim 1, it is characterised in that: described pressing plate (21) bottom
It is equipped with the first elastomeric layer (22) with pressing plate through hole (23) inner side.
Apparatus for combining liquids the most according to claim 1, it is characterised in that: set on described second sealing member
The second elastomeric layer, the second elastomeric layer is had to be sealed by the bottom opening of reagent container (81).
Apparatus for combining liquids the most according to claim 1, it is characterised in that: described cover plate (11) bottom
Being provided with projection (12), cover plate (11) covers when pressing plate (21) is upper, and protruding (12) embed pressing plate through hole (23)
In.
Apparatus for combining liquids the most according to claim 1, it is characterised in that: described pressing plate through hole (23)
Cross-sectional area less than reagent container (81) open-topped cross-sectional area.
Apparatus for combining liquids the most according to claim 1, it is characterised in that: described reagent container (81)
The inwall of bottom is provided with the boss extended internally.
Apparatus for combining liquids the most according to claim 1, it is characterised in that: described reagent container (81)
Top accommodates chamber (91) top less than reagent, and the thickness of described pressing plate (21) is equal to reagent container (81) top
The distance between chamber (91) top is accommodated to reagent.
Apparatus for combining liquids the most according to claim 1, it is characterised in that: described reagent accommodates chamber (91)
Sidewall be provided with reagent accommodate Handheld Division, chamber (32), reagent accommodate Handheld Division, chamber be provided with the first groove (33).
Apparatus for combining liquids the most according to claim 1, it is characterised in that: set on described second sealing member
Having the second sealing member Handheld Division (41), the second sealing member Handheld Division is provided with the second groove (43).
10. use apparatus for combining liquids as in one of claimed in any of claims 1 to 9 to carry out the side of liquid mixing
Method, it is characterised in that: comprise the following steps:
S1: reagent receiving chamber (91) encapsulates reagent in advance, removes cover plate (11), extract the second sealing member out,
In hybrid chamber (61), sample is added by adding mouth (52);
S2: by pressing plate through hole (23) to reagent container (81) inner inflatable, by reagent container (81)
Reagent is pressed in hybrid chamber (61) via passage (51), thus is mixed with sample by reagent.
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201610525481.7A CN105938066B (en) | 2016-07-05 | 2016-07-05 | Apparatus for combining liquids and its mixed method |
DE202016104521.5U DE202016104521U1 (en) | 2016-07-05 | 2016-08-17 | Liquid mixer |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN201610525481.7A CN105938066B (en) | 2016-07-05 | 2016-07-05 | Apparatus for combining liquids and its mixed method |
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CN105938066A true CN105938066A (en) | 2016-09-14 |
CN105938066B CN105938066B (en) | 2017-07-18 |
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CN201610525481.7A Active CN105938066B (en) | 2016-07-05 | 2016-07-05 | Apparatus for combining liquids and its mixed method |
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DE (1) | DE202016104521U1 (en) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108535084A (en) * | 2018-04-08 | 2018-09-14 | 河南农业大学 | A kind of liquid mixing chamber |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
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CN111855998B (en) * | 2020-08-04 | 2024-06-14 | 高伟 | Sealed specimen processing device and application method thereof |
CN114870701A (en) * | 2022-05-10 | 2022-08-09 | 山东威高血液净化制品股份有限公司 | Vortex oscillation mixing device |
Citations (6)
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DE68925370D1 (en) * | 1988-02-16 | 1996-02-22 | Rms Lab Inc | Apparatus for luminescence detection and exposure |
CN101048336A (en) * | 2004-10-21 | 2007-10-03 | 环球农业技术及工程有限公司 | Liquid mixture dispensing system |
JP2008246363A (en) * | 2007-03-30 | 2008-10-16 | Doshisha | Multiple dispenser |
CN204649517U (en) * | 2015-05-26 | 2015-09-16 | 辽宁中医药大学 | Stirring apparatus in a kind of automatic sampler |
CN105107449A (en) * | 2015-09-11 | 2015-12-02 | 南京天纵易康生物科技股份有限公司 | Combined type reaction cup |
CN205879656U (en) * | 2016-07-05 | 2017-01-11 | 南京天纵易康生物科技股份有限公司 | Liquid mixer |
-
2016
- 2016-07-05 CN CN201610525481.7A patent/CN105938066B/en active Active
- 2016-08-17 DE DE202016104521.5U patent/DE202016104521U1/en active Active
Patent Citations (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE68925370D1 (en) * | 1988-02-16 | 1996-02-22 | Rms Lab Inc | Apparatus for luminescence detection and exposure |
CN101048336A (en) * | 2004-10-21 | 2007-10-03 | 环球农业技术及工程有限公司 | Liquid mixture dispensing system |
JP2008246363A (en) * | 2007-03-30 | 2008-10-16 | Doshisha | Multiple dispenser |
CN204649517U (en) * | 2015-05-26 | 2015-09-16 | 辽宁中医药大学 | Stirring apparatus in a kind of automatic sampler |
CN105107449A (en) * | 2015-09-11 | 2015-12-02 | 南京天纵易康生物科技股份有限公司 | Combined type reaction cup |
CN205879656U (en) * | 2016-07-05 | 2017-01-11 | 南京天纵易康生物科技股份有限公司 | Liquid mixer |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN108535084A (en) * | 2018-04-08 | 2018-09-14 | 河南农业大学 | A kind of liquid mixing chamber |
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Publication number | Publication date |
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CN105938066B (en) | 2017-07-18 |
DE202016104521U1 (en) | 2016-08-29 |
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Effective date of registration: 20220607 Address after: 410000 room 102-2, 1st floor, building A5, phase II, Changsha E center, No. 18, Xiangtai Road, Liuyang economic and Technological Development Zone, Changsha City, Hunan Province Patentee after: Hunan Tianzong Yijun Biotechnology Co.,Ltd. Address before: 210000 floors 1-3, Block E, Dingye Baitai building, Xinghuo Road, Pukou high tech Zone, Nanjing, Jiangsu Patentee before: Nanjing Tzong Blotechnology Co.,Ltd. |