CN117286059B - Composite microbial inoculum, microbial synergist, microbial compound fertilizer and application - Google Patents

Composite microbial inoculum, microbial synergist, microbial compound fertilizer and application Download PDF

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CN117286059B
CN117286059B CN202311190566.0A CN202311190566A CN117286059B CN 117286059 B CN117286059 B CN 117286059B CN 202311190566 A CN202311190566 A CN 202311190566A CN 117286059 B CN117286059 B CN 117286059B
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microbial
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bacteria
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CN117286059A (en
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韩金波
董肖杰
公茂斌
王敏欣
王云超
常冬
冀定磊
李延锋
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Sinochem Agriculture Holdings
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
    • C05F11/00—Other organic fertilisers
    • C05F11/08—Organic fertilisers containing added bacterial cultures, mycelia or the like
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
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    • C12R2001/07—Bacillus
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/645—Fungi ; Processes using fungi
    • C12R2001/885—Trichoderma

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Abstract

The invention relates to the field of fertilizers, in particular to a composite microbial inoculant, a microbial synergist, a microbial compound fertilizer and application. The composite microbial inoculum is prepared by mixing 5 types of strains (4 bacteria in spore form and 1 fungus spore), and has remarkable antibacterial effect on fusarium graminearum. The composite microbial inoculum and the synergistic auxiliary agent provided by the invention are jointly applied to cereal crops, so that the cereal crop diseases and the toxin content in cereal crop kernels can be obviously reduced, and the composite microbial inoculum has great application significance in the aspects of grain production and grain safety.

Description

Composite microbial inoculum, microbial synergist, microbial compound fertilizer and application
Technical Field
The invention relates to the field of fertilizers, in particular to a composite microbial inoculant, a microbial synergist, a microbial compound fertilizer and application.
Background
Cereal crops as an important grain source face a number of problems due to the influence of soil conditions, plant diseases and insect pests, environmental conditions (light, temperature, water, etc.), harvest, etc. Among these, the most significant problem is mycotoxin contamination. Mycotoxins are extremely pathogenic, not only causing reduced yields and reduced quality of raw grain, but also jeopardizing the health of humans and animals and even leading to death.
Vomitoxin, gibberellin, aflatoxin are among the most common mycotoxins that affect most. Wherein, aflatoxin can reduce toxicity by decomposition or adsorption, and vomitoxin has no mature and effective degradation method. The excessive vomitoxin content in the seeds is mainly influenced by factors such as variety, temperature, humidity, harvesting time, seed breakage rate during harvesting and the like, and in the planting process, the control of insect damage such as borers and fusarium infection is an effective measure for preventing the excessive vomitoxin content, but no technical product for completely and effectively controlling the toxin content is available at present, and an application technology capable of obviously reducing the toxin content of the seeds is urgently needed.
In view of this, the present invention has been made.
Disclosure of Invention
In order to solve the technical problems, the invention provides a composite microbial inoculant, a microbial synergist, a microbial compound fertilizer and application. The composite microbial inoculum is prepared by mixing 5 types of strains (4 bacteria in spore form and 1 fungus spore), and has remarkable antibacterial effect on fusarium graminearum. The composite microbial inoculum provided by the invention and the synergistic auxiliary agent are jointly applied to cereal crops, so that cereal crop diseases and toxin content in cereal crop kernels can be obviously reduced.
Specifically, the technical scheme of the invention is as follows:
in a first aspect, the invention provides a composite microbial inoculant, which comprises, by mass, 20-50 parts of bacillus subtilis, 10-30 parts of bacillus mucilaginosus, 10-30 parts of bacillus amyloliquefaciens, 10-35 parts of bacillus laterosporus and 10-50 parts of trichoderma harzianum.
Wherein the amount of the bacillus subtilis is 20-50 parts, preferably 25-50 parts, more preferably 25-30 parts. The Bacillus mucilaginosus is used in an amount of 10 to 30 parts, preferably 10 to 20 parts, more preferably 10 to 15 parts. The Bacillus amyloliquefaciens is used in an amount of 10 to 30 parts, preferably 10 to 20 parts, more preferably 10 to 15 parts. The amount of Bacillus laterosporus is 10-35 parts, preferably 10-20 parts, more preferably 15-20 parts. The amount of Trichoderma harzianum is 10-50 parts, preferably 10-30 parts, more preferably 20-30 parts.
According to the invention, 5 types of strains are compounded in the proportion to obtain the composite microbial inoculum, and all the strains are matched with each other, so that the composite microbial inoculum has a remarkable inhibition effect on fusarium graminearum. And when the bacteria are compounded in the preferable dosage parts, the obtained composite microbial inoculum has obviously better inhibition effect on fusarium graminearum. The specific sources of the above 5 species are not particularly limited, and conventional commercial sources in the art may be used. Wherein, the form of the class 4 bacteria in the process of compounding is preferably spore; the form of the class 1 fungus upon reconstitution is preferably spores.
In a second aspect, the invention provides a bacteria-containing synergist, which comprises the composite bacterial agent and a synergist.
In the invention, the synergistic additive is a fertilizer synergistic agent which is commonly used for plant planting in the field and has the effect of promoting plant height, stem thickness, fresh weight on the ground and the like, such as chitosan oligosaccharide, polyglutamic acid, seaweed active element, seaweed polysaccharide, spring element, brown seaweed oligosaccharide, mineral fulvic acid, aminobutyric acid, fish protein peptide, yellow peptide, synergistic nitrogen, synergistic phosphorus, amino acid, plant element, benefit applying side and the like.
In a more preferred embodiment of the present invention, the synergistic adjuvant is selected from at least two of the aforementioned chitosan oligosaccharide, polyglutamic acid, seaweed active ingredient, seaweed polysaccharide, spring ingredient, brown algae oligosaccharide, mineral fulvic acid, aminobutyric acid, fish protein peptide, xanthene peptide, synergistic nitrogen, synergistic phosphorus, amino acid, plant ingredient, and benefit applicator.
More preferably, the synergistic agent is algal polysaccharide and polyglutamic acid.
The invention researches the influence of different synergistic assistants on the growth of cereal crops (corn varieties: artemisia princeps) in the early stage, and specifically comprises the following steps:
test crop: corn pot (artificial climate greenhouse, day: night=12:12);
The treatment method comprises the following steps: the dosage of the base fertilizer is equal; after weighing the synergist, diluting the synergist in an equal volume to prepare a synergist solution, quantitatively adding each treatment by a pipetting gun, and repeating 5 basins for each treatment. 6 seeds are sowed in each pot, and after seedlings emerge until the seedlings are aligned, 2 seedlings with consistent growth vigor are selected and reserved in each pot. Culturing in artificial greenhouse.
The growth promoting effect of different treatments on the plant height, the stem thickness and the fresh weight of the maize seedlings is measured 40 days after the treatment. The effect of different synergists on the fresh weight control effect of the Artemisia princeps Pampanini is shown in Table 1.
TABLE 1 influence of co-agent addition on fresh weight control of Artemisia princeps Pampanini
Treatment of Synergistic auxiliary agent Dosage g/mu Plant height cm Thick stem mm Fresh weight g on ground Underground fresh weight g
T1 Clean water 80 69.00 8.00 25.17 4.14
T2 Chitosan oligosaccharide 20 80.00 9.35 35.03 6.37
T3 Polyglutamic acid 40 84.25 9.46 36.93 7.05
T4 Seaweed vitality 80 83.67 9.79 36.03 5.98
T5 Seaweed polysaccharide 80 86.50 9.54 37.10 6.35
T6 Spring essence 20 78.38 9.57 36.30 6.63
T7 Brown algae oligosaccharide 12 81.42 9.42 33.83 5.72
T8 Mining source fulvic acid 240 78.92 9.40 33.38 5.78
T9 Aminobutyric acid 40 74.50 8.23 27.88 5.15
T10 Fish protein peptide 80 76.25 8.57 28.83 4.87
T11 Yellow peptide 20 72.25 7.91 25.50 4.00
T12 Synergistic nitrogen 400 70.00 8.00 30.12 4.42
T13 Synergistic phosphorus 160 73.50 8.93 31.48 4.18
T14 Amino acids 400 66.00 6.94 18.60 3.14
T15 Phytochemicals 40 73.08 8.36 26.20 4.28
T16 Benefit applying upper 100 69.75 7.50 21.65 3.23
The seaweed polysaccharide and the polyglutamic acid have obvious promotion effects on plant height, thick stems, overground fresh weight and underground fresh weight of cereal crops, and have obvious advantages compared with other types of synergistic assistants. Wherein, the seaweed polysaccharide can improve the activity of plant cells, enhance the stress resistance capability of freeze injury, drought and waterlogging and the like, and reduce the occurrence of diseases. The seaweed polysaccharide is generally used in an amount of 50-100 g/mu, preferably 80 g/mu. The effect of the seaweed polysaccharide on the fresh weight of the cereal crop on the ground is more obvious (in the experiment of potting corn seed seedlings, clear water is used as a control, 80 g/mu of seaweed polysaccharide is added, and the fresh weight of the cereal crop on the ground after 40 days of treatment is improved to 37.10g compared with 25.17g of the control group). The amount of polyglutamic acid is generally 20-60 g/mu, preferably 40 g/mu. The underground fresh weight of the polyglutamic acid on cereal crops is better (in the experiment of potting corn seeds and seedlings, clear water is used as a reference, 40 g/mu of polyglutamic acid is added, and the underground fresh weight of the cereal crops after 40 days of treatment is improved to 7.05g compared with 4.14g of the reference group).
Other synergistic additives such as chitosan oligosaccharide, seaweed vitality element, spring element and the like have obvious synergistic effects (superior to other conventional synergistic additives) in the aspects of plant height, stem thickness, overground fresh weight and underground fresh weight of cereal crops, and partial indexes (such as stem thickness, underground fresh weight and the like) are superior to seaweed polysaccharide and polyglutamic acid. However, in the further cereal crop disease research and the research on the content of toxin in seeds, the performance of synergistic additives such as chitosan oligosaccharide, seaweed vitality element, spring element and the like is obviously weaker than that of seaweed polysaccharide and polyglutamic acid.
In the invention, the mass ratio of the composite microbial inoculum to the synergistic agent is preferably 1-3:1-10.
When the synergistic agent is algal polysaccharide and polyglutamic acid, the mass ratio of the complex bacterial agent to the algal polysaccharide to the polyglutamic acid is preferably 1-2:1-2:1-2, and more preferably 1:1:1.
The invention does not limit the specific category and source of the synergistic agent, and various synergistic agents from conventional commercial sources in the field can be used.
In a third aspect, the invention provides a bacteria-containing compound fertilizer, which comprises the bacteria-containing synergistic agent and fertilizer.
The specific type and source of the fertilizer are not particularly limited in the present invention, and fertilizers for cereal crops, which are conventionally commercially available in the art, may be used.
In a preferred embodiment of the invention, the fertilizer has an available nutrient N-P 2O5-K2 O content of 15-15-15.
In the invention, the mass ratio of the composite microbial inoculum to the fertilizer is preferably 1-3:500-2000, more preferably 1-3:1000.
The microbial compound fertilizer obtained by matching the preferred proportion comprises a microbial inoculum, a synergistic auxiliary agent and fertilizer nutrients, and has better effect in reducing ear rot and seed toxin after the components are compounded according to the proportion.
In a more specific embodiment of the invention, the bacteria-containing compound fertilizer is prepared by compounding the composite microbial inoculum, the synergistic auxiliary agent and the fertilizer according to the mass ratio of 1-3:1-10:1000, and has remarkable effect in reducing cereal crop diseases and the content of toxin in cereal crop grains when being used as a cereal crop base fertilizer.
In a fourth aspect, the invention provides a preparation method of the bacteria-containing compound fertilizer, which comprises the steps of weighing and mixing raw material components according to parts by mass to obtain the bacteria-containing compound fertilizer.
The preparation method provided by the invention is simple and efficient, and is suitable for industrial production.
In a fifth aspect, the invention provides the application of the composite microbial inoculum, or the microbial synergist or the microbial compound fertilizer in cereal crop planting.
Preferably, the cereal crop is selected from the group consisting of maize, wheat, rice;
preferably, the application comprises at least one of:
(1) The method is used for preparing compound fertilizers, blending fertilizers or organic fertilizers;
(2) For reducing cereal crop disease; the cereal crop diseases include ear rot;
(3) For reducing the toxin content in cereal crop kernels.
The beneficial effects are that:
The invention provides a composite microbial inoculum, a microbial synergist, a microbial compound fertilizer and application thereof. The composite microbial inoculum is prepared by mixing 5 types of strains (4 bacteria in spore form and 1 fungus spore), and has remarkable antibacterial effect on fusarium graminearum. The composite microbial inoculum and the synergistic auxiliary agent provided by the invention are jointly applied to cereal crops, so that the cereal crop diseases and the toxin content in cereal crop kernels can be obviously reduced, and the composite microbial inoculum has great application significance in the aspects of grain production and grain safety.
Drawings
In order to more clearly illustrate the technical solutions of the present invention or the prior art, the drawings used in the description of the embodiments or the prior art will be described below.
Fig. 1 shows the results of the experiment of the bacteriostatic effect of the different composite microbial agents in example 1 of the present invention.
Detailed Description
The technical solutions provided by the present invention are described in detail below with reference to examples, but they should not be construed as limiting the scope of the present invention. Unless otherwise indicated, all the experimental procedures used in the examples were conventional; the materials, reagents and the like used are all commercially available.
Example 1
The embodiment provides a composite microbial inoculant, which comprises the following components: 25 parts of bacillus subtilis, 10 parts of bacillus mucilaginosus, 15 parts of bacillus amyloliquefaciens, 20 parts of bacillus laterosporus and 30 parts of trichoderma harzianum.
The embodiment also provides a preparation method of the composite microbial inoculum, which comprises the following steps: weighing spores or spores of various strains according to mass parts, and mixing to obtain the composite microbial inoculum.
Example 2
The embodiment provides a composite microbial inoculant, which comprises the following components: 30 parts of bacillus subtilis, 20 parts of bacillus mucilaginosus, 10 parts of bacillus amyloliquefaciens, 20 parts of bacillus laterosporus and 20 parts of trichoderma harzianum.
The preparation method of the composite microbial inoculum is the same as that of the embodiment 1.
Example 3
The embodiment provides a composite microbial inoculant, which comprises the following components: 50 parts of bacillus subtilis, 10 parts of bacillus mucilaginosus, 20 parts of bacillus amyloliquefaciens, 10 parts of bacillus laterosporus and 10 parts of trichoderma harzianum.
The preparation method of the composite microbial inoculum is the same as that of the embodiment 1.
Example 4
The embodiment provides a composite microbial inoculant, which comprises the following components: 20 parts of bacillus subtilis, 20 parts of bacillus mucilaginosus, 10 parts of bacillus amyloliquefaciens, 20 parts of bacillus laterosporus and 30 parts of trichoderma harzianum.
The preparation method of the composite microbial inoculum is the same as that of the embodiment 1.
Example 5
The embodiment provides a composite microbial inoculant, which comprises the following components: 40 parts of bacillus subtilis, 10 parts of bacillus mucilaginosus, 30 parts of bacillus amyloliquefaciens, 10 parts of bacillus laterosporus and 10 parts of trichoderma harzianum.
The preparation method of the composite microbial inoculum is the same as that of the embodiment 1.
Comparative example 1
The comparative example provides a compound microbial inoculum, which comprises the following formula: 25 parts of bacillus subtilis, 10 parts of bacillus mucilaginosus, 15 parts of bacillus amyloliquefaciens, 20 parts of bacillus laterosporus and 30 parts of conidium.
The preparation method of the composite microbial inoculum of the comparative example is the same as that of example 1.
Comparative example 2
The comparative example provides a compound microbial inoculum, which comprises the following formula: 25 parts of pseudomonas, 10 parts of bacillus mucilaginosus, 15 parts of bacillus amyloliquefaciens, 20 parts of bacillus laterosporus and 30 parts of trichoderma harzianum.
The preparation method of the composite microbial inoculum of the comparative example is the same as that of example 1.
Comparative example 3
The comparative example provides a compound microbial inoculum, which comprises the following formula: 25 parts of chaetomium, 10 parts of bacillus mucilaginosus, 15 parts of bacillus amyloliquefaciens, 20 parts of bacillus laterosporus and 30 parts of conidium.
The preparation method of the composite microbial inoculum of the comparative example is the same as that of example 1.
Experimental example 1
The composite microbial agents described in examples 1 to 5 and comparative examples 1 to 3 were selected in this experimental example, and the fusarium graminearum inhibition effect of each composite microbial agent was compared.
The test method comprises the following steps: the plates were inoculated with the composite microbial inoculum and Fusarium graminearum (1 g of each composite microbial inoculum was diluted 1000 times, 10uL was aspirated for coating, fusarium graminearum was inoculated with a bacterial cake having a diameter of 0.5 cm), and the culture was carried out in an artificial incubator at 28℃for 5 days for observation.
The bacteriostatic effect of the complex microbial agents described in examples 1-5 and comparative examples 1-3 is shown in FIG. 1.
In FIG. 1, the two sides of the culture dish are provided with composite microbial inoculum, and the middle is provided with pathogenic bacteria; the first row is the bacteriostatic effect on the front surface of the culture dish, and the second and third rows are the bacteriostatic effect on the back surface of the culture dish (two groups of repetition).
As can be seen from fig. 1: the composite microbial inoculum described in examples 1-5 has obvious inhibition effect on growth of fusarium graminearum. Among them, the zone of inhibition of example 1 was the largest and the best effect. The compound bacteria of examples 2-5 have the bacteria ratio adjusted, and the inhibition zone is smaller than that of example 1, but has obvious inhibition performance. The bacteriostatic effect of examples 2-4 was better than that of example 5. Comparative examples 1 to 3 each were subjected to adjustment of species types constituting the composite microbial inoculum. Wherein, the antibacterial effect of comparative example 1 is not obvious; the bacteriostatic effect of comparative example 2 was not as good as that of examples 1-5; comparative example 3, although showing a better bacteriostatic effect, is inferior to examples 1 to 4.
Example 6
The embodiment provides a bacteria-containing synergist, which comprises the following components in percentage by weight:
60 parts of composite microbial inoculum (prepared in example 1), 60 parts of algal polysaccharide and 60 parts of polyglutamic acid.
The embodiment also provides a preparation method of the bacteria-containing synergist, which comprises the following steps: weighing the raw materials according to the mass parts, and mixing to obtain the bacteria-containing synergist.
Example 7
The embodiment provides a bacteria-containing synergist, which comprises the following components in percentage by weight:
60 parts of composite microbial inoculum (prepared in example 1), 60 parts of alginic acid and 60 parts of mineral source fulvic acid.
The preparation method of the bacteria-containing synergist in this example is the same as that in example 6.
Example 8
The embodiment provides a bacteria-containing synergist, which comprises the following components in percentage by weight:
60 parts of composite microbial inoculum (prepared in example 1), 60 parts of seaweed vitality element and 60 parts of mineral source fulvic acid.
The preparation method of the bacteria-containing synergist in this example is the same as that in example 6.
Example 9
The embodiment provides a bacteria-containing synergist, which comprises the following components in percentage by weight:
60 parts of composite microbial inoculum (prepared in example 1), 60 parts of polyglutamic acid and 60 parts of chitosan oligosaccharide.
The preparation method of the bacteria-containing synergist in this example is the same as that in example 6.
Experimental example 2
The fungus-containing synergistic agent described in examples 6-9 is selected for the experimental example to prepare the fungus-containing compound fertilizer. And then comparing and testing the influence condition of corn ear rot and seed toxin content of each bacteria-containing compound fertilizer.
Test crop: corn, variety: YF3240.
The test method comprises the following steps:
T1 treatment group (CK): applying 40kg of base fertilizer.
T2 treatment group: 40kg of base fertilizer is applied, and 180g of the bacteria-containing synergist (60 g of composite bacteria agent, 60g of algal polysaccharide and 60g of polyglutamic acid) described in the example 6 is added.
T3 treatment group: applying 40kg of base fertilizer and simultaneously increasing 180g of the microbial synergist (60 g of composite microbial inoculant, 60g of alginic acid and 60g of mineral source fulvic acid) in example 7.
T4 treatment group: applying 40kg of base fertilizer and simultaneously increasing 180g of the bacteria-containing synergist (60 g of composite bacteria agent, 60g of seaweed vitality element and 60g of mineral source fulvic acid) in example 8.
T5 treatment group: 40kg of base fertilizer is applied, and 180g of the bacteria-containing synergist (60 g of composite bacteria agent, 60g of polyglutamic acid and 60g of chitosan oligosaccharide) described in the example 9 is added.
The effective nutrient content of the base fertilizer in the T1-T5 group is N-P 2O5-K2 O (15-15-15). And the microbial synergist in the T2-T5 treatment group is coated on the surface of the base fertilizer particles to prepare a microbial compound fertilizer, then the microbial compound fertilizer is co-sowed with corn seed fertilizer, the corn seed is ripened until the corn seed is ripened, the spike rot prevention effect is measured, and the toxin content is measured after the corn seed is harvested. The results are shown in Table 2.
TABLE 2 influence of the microbial Compound fertilizer on corn ear rot and seed toxin content (ug/kg)
From the test results in Table 2, it can be seen that: the occurrence degree of each treatment spike rot is moderate, and the vomitoxin of the corn kernel is detected, and the gibberellin ketone and the aflatoxin are detected in part of the samples. The bacteria-containing compound fertilizer can obviously reduce 18-57% of corn post-spike rot and 17-70% of seed vomit toxin. The content of gibberellin ketone and aflatoxin is reduced to a certain extent. After the synergistic auxiliary agent is combined with the composite microbial inoculum, the corn growth can be obviously promoted, and the later-stage yield is improved by 11-12%. Has better economic benefit. Comprehensive comparison of T1-T5 shows that T2 treatment has optimal yield increase and lower toxin content at different test points.
The above examples merely represent a few embodiments of the present invention, which facilitate a specific and detailed understanding of the technical solutions of the present invention, but are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention.

Claims (7)

1. The bacteria-containing synergist is characterized by comprising a composite bacterial agent and a synergistic auxiliary agent; the composite microbial inoculum consists of, by mass, 25 parts of bacillus subtilis, 10 parts of bacillus mucilaginosus, 15 parts of bacillus amyloliquefaciens, 20 parts of bacillus laterosporus and 30 parts of trichoderma harzianum; the synergistic agent is algal polysaccharide and polyglutamic acid; the mass ratio of the composite microbial inoculum to the algal polysaccharide to the polyglutamic acid is 1:1:1.
2. The bacteria-containing compound fertilizer is characterized by comprising the bacteria-containing synergistic agent of claim 1 and further comprising a fertilizer; the mass ratio of the composite microbial inoculum in the microbial compound fertilizer to the fertilizer is 1-3:500-2000.
3. The preparation method of the bacteria-containing compound fertilizer of claim 2, wherein the bacteria-containing compound fertilizer is obtained by weighing and mixing the raw material components according to the mass parts.
4. Use of the microbial synergist of claim 1 or the microbial compound fertilizer of claim 2 in corn planting.
5. The use of the microbial synergist of claim 1 in the preparation of a compound fertilizer, a blended fertilizer or an organic fertilizer.
6. The use of the microbial synergist of claim 1 or the microbial compound fertilizer of claim 2 for reducing corn ear rot.
7. Use of the microbial synergist of claim 1 or the microbial compound fertilizer of claim 2 for reducing the toxin content in corn kernels, said toxin being selected from at least one of vomitoxin, gibberellin, and aflatoxin.
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Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107673927A (en) * 2017-11-03 2018-02-09 黑龙江省农业科学院土壤肥料与环境资源研究所 A kind of rice slow-release fertilizer and preparation method thereof
CN109182172A (en) * 2018-09-06 2019-01-11 河南天赐鼎立农业科技有限公司 A kind of promoting root growth supports root, increasing yield and improving quality complex micro organism fungicide and preparation method thereof
CN109749974A (en) * 2019-03-21 2019-05-14 沈阳师范大学 A kind of Bacillus amyloliquefaciens strain and its application
CN109776154A (en) * 2017-11-13 2019-05-21 深圳市芭田生态工程股份有限公司 Fertilizer synergistic composition, its application and fertilizer
CN113788725A (en) * 2021-09-03 2021-12-14 哈尔滨帝盟生物科技有限公司 Multifunctional fertilizer synergist and preparation method thereof
CN114672440A (en) * 2022-04-26 2022-06-28 中国水稻研究所 Bacillus subtilis BS273 and application thereof
CN115925486A (en) * 2023-02-06 2023-04-07 青岛海大生物集团股份有限公司 Seaweed compound microbial fertilizer for preventing and treating soil moss and preparation method thereof

Patent Citations (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN107673927A (en) * 2017-11-03 2018-02-09 黑龙江省农业科学院土壤肥料与环境资源研究所 A kind of rice slow-release fertilizer and preparation method thereof
CN109776154A (en) * 2017-11-13 2019-05-21 深圳市芭田生态工程股份有限公司 Fertilizer synergistic composition, its application and fertilizer
CN109182172A (en) * 2018-09-06 2019-01-11 河南天赐鼎立农业科技有限公司 A kind of promoting root growth supports root, increasing yield and improving quality complex micro organism fungicide and preparation method thereof
CN109749974A (en) * 2019-03-21 2019-05-14 沈阳师范大学 A kind of Bacillus amyloliquefaciens strain and its application
CN113788725A (en) * 2021-09-03 2021-12-14 哈尔滨帝盟生物科技有限公司 Multifunctional fertilizer synergist and preparation method thereof
CN114672440A (en) * 2022-04-26 2022-06-28 中国水稻研究所 Bacillus subtilis BS273 and application thereof
CN115925486A (en) * 2023-02-06 2023-04-07 青岛海大生物集团股份有限公司 Seaweed compound microbial fertilizer for preventing and treating soil moss and preparation method thereof

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
哈茨木霉生物防治研究进展;吕黎 等;《湖南农业科学》;20130915(第17期);第94页第1栏第2段 *

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