CN101485337B - Agent for propagating Trichoderma sp. in field - Google Patents
Agent for propagating Trichoderma sp. in field Download PDFInfo
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- CN101485337B CN101485337B CN2009100958768A CN200910095876A CN101485337B CN 101485337 B CN101485337 B CN 101485337B CN 2009100958768 A CN2009100958768 A CN 2009100958768A CN 200910095876 A CN200910095876 A CN 200910095876A CN 101485337 B CN101485337 B CN 101485337B
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Abstract
The invention discloses a trichoderma field proliferating agent, and belongs to application of microorganisms. The prior product has the defects of complex application method, higher cost, unobvious effect and the like. The trichoderma field proliferating agent is prepared by uniformly mixing a trichoderma fungus spore agent and a nutrient medium, wherein the trichoderma fungus spore agent is solid or a liquid or a mixture of the solid and the liquid, which is prepared by culturing trichoderma fungi and contains a large quantity of trichoderma fungus spores and a small quantity of viable mycelium. The trichoderma field proliferating agent is applied on the humid surface of field soil, and nutrition is supplied to the trichoderma fungi by the nutrient medium, so that the trichoderma fungi are grown and proliferated on the soil surface and not only can prevent fungous diseases on roots and caudexes of plants but also can prevent fungous diseases on stems, leaves, flowers and fruits of the plants. The trichoderma field proliferating agent also has certain production increase function on crops after being applied.
Description
Technical field
The invention belongs to the microorganism application, relate in particular to a kind of product that utilizes trichoderma (Trichoderma) fungi prevention fungal diseases of plants.
Background technology
The research that utilizes wood mould (Trichoderma) fungi antagonism plant disease fungi is since existing so far nearly 80 years history thirties in 20th century.
Studies confirm that, wood mould (Trichoderma) fungi is plurality of plant diseases fungies such as antagonism sickle-like bacteria, southern blight bacterium, rhizoctonia, phytophthora, Rhizoctonia solani Kuhn, botrytis cinerea, Botrytis cinerea bacterium and wheat glume blight bacterium effectively, reach the purpose of prevention plant disease with this.Wooden mould fungi is used in the field, and the anxiety that no chemical pesticide is residual is specially adapted to the pollution-less agriculture field.
After the antagonism mechanism of wood mould (Trichoderma) fungi was extensively admitted, both at home and abroad expert, scholar were devoted to the work that wooden mould fungi is applied to agricultural production and do one's utmost, but fail so far to be widely used on agricultural.Five aspects of having traced it to its cause:
1, the form of preparation, existing most product is the conidial powder preparation, on application process mainly is to mix soil to spread fertilizer over the fields, directly admix plow layer, plant plant hole and spray.Mix soil spreads fertilizer over the fields, directly admixes plow layer, the application process of planting plant hole is subjected to the antibacterial factor in the soil influence, spray the influence that is subjected to weather and environment, wooden mould spore germination, mycelial growth and grow all undesirable surely.And, the part inactivation is arranged in the transportation of commodity preparation with in storing because the spore of fungi becomes current shorter, and under the situation that the spore amount of living reduces, the spore germination after above-mentioned application process influence is used, mycelial growth and growing surely;
2, notion is indeterminate, and industry is many at present says that becoming is a kind of biology of controlling plant diseases to wooden mould fungi, and the mould fungi of its solid wood can only be the prevention plant disease, and fungi wood is mould can not cure the plant that has fallen ill;
3, application process is complicated, and agriculture practical application is had any problem;
4, cost is higher;
5, DeGrain.
Summary of the invention
The technical assignment of the technical problem to be solved in the present invention and proposition is to overcome complicated, the shortcomings such as cost is higher, DeGrain of application process that prior art exists, and provides a kind of wood mould field proliferating agent.For this reason, the present invention is by the following technical solutions:
The mould field proliferating agent of wood, it is characterized in that evenly being mixed by mould fungal spore agent of wood and nutrition base and form, the mould fungal spore agent of described wood is the solid or liquid or solid and the mixtures of liquids that contain the mould fungal spore of a large amount of wood and a small amount of viable bacteria filament to the mould fungal culture gained of wood.Described nutrition base is for growing, produce the nutriment that spore provides carbon, nitrogen and mineral matter and trace element fast for the mould fungi of wood; The proportioning of mould fungal spore agent of described wood and nutrition base is:
A, when the mould fungal spore agent drying of solid wood through cultivating gained is crushed into conidial powder, the percentage by weight of conidial powder is 25%～75%, all the other are the nutrition base of drying pulverizing;
B, when the mould fungal spore agent of liquid wood through cultivating gained is spore liquid, the percentage by weight of spore liquid is 30%～70%, all the other are the nutrition base of drying pulverizing;
C, when conidial powder and spore liquid are mixed into wooden mould fungal spore agent, the percentage by weight of conidial powder and spore liquid is 25%～75%, all the other are the nutrition base that drying is pulverized.
The invention has the beneficial effects as follows:
Product of the present invention is applied in moistening field soil surface, the mould fungal spore fast-germination of wood, the nutrition base is that wooden mould conk is supplied with the prescribing adequate nutrition material, and wooden mould fungi plays the effect of prevention crop root, basal part of stem fungal disease in raised growth, the breeding fast of damp soil surface; The mould fungi of wood produces a large amount of conidiums and spreads in the field in damp soil superficial growth, reproductive process, these fresh and alive wooden mould spores are diffused into the soil position germination and growth again that does not spread this product, reach the effect of comprehensive prevention crop root, basal part of stem fungal disease.
Further, spread fertilizer over the fields the fungal disease that product of the present invention can prevent stem of plant, leaf, flower, fruit position on the damp soil surface.This is big characteristics of product of the present invention.
The mould fungi of wood damp soil superficial growth, breeding at first suppress soil surface may damage to crops the sprouting of disease fungal spore of stem, leaf, flower, fruit and the growth of mycelia, reduce the dynamics that infects of disease fungi.The mould fungi of wood produces a large amount of spores and discharges, disperses in the field in soil surface growth, reproductive process, the part spore can land in stem of plant, leaf, flower, fruit may be sprouted by the position that the disease fungi encroaches on, growth, play a protective role; The mould fungi of wood can discharge a large amount of volatility antibiotic in damp soil superficial growth, reproductive process, can suppress soil surface and stem of plant, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
The mould field proliferating agent of wood is used the back crop is also had certain effect of increasing production.
Embodiment
Below specify the mould field proliferating agent of wood of the present invention.
The mould field proliferating agent of wood, evenly mixed by mould fungal spore agent of wood and nutrition base and to form, wooden mould fungal spore agent is the solid or liquid or solid and the mixtures of liquids that contain the mould fungal spore of a large amount of wood and a small amount of viable bacteria filament to the mould fungal culture gained of wood.The nutrition base is for growing, produce the nutriment that spore provides carbon, nitrogen and mineral matter and trace element fast for the mould fungi of wood.Before mixing with wooden mould fungal spore agent, the nutrition base under 120 ± 5 ℃ of conditions, sterilized 30 ± 5 minutes.
The mould fungi of the used wood of the present invention (Trichoderma) belongs to the trichoderma of Fungi Imperfecti.It is in the fungus Trichoderma (, koning trichoderma mould, mould, the yellow green trichoderma of hook-shaped wood and tangerine green trichoderma etc.) as Trichoderma harzianum, Trichoderma viride, long shoot wood one or both combination or several combination.
The mould fungal spore of described wood is conidium or chlamydospore or conidium and chlamydosporic combination.It is with following material as medium to the mould fungal culture gained of wood:
A, grain (as rice, broomcorn millet, millet, wheat, bean, corn etc.) seed are or/and its by product; Perhaps
The branch of B, plant, leaf, root, flower or fruit, perhaps its mixture; Perhaps
The skin of C, animal, hair or meat, perhaps its mixture; Perhaps
Two kinds combination among three kinds of A, B, the C or several combination.
As required, can in medium, add mineral matter.
Cultivation, the expansion of the mould fungi of wood is numerous, processing method can be conventional solid culture, liquid culture or other cultural method, also can be that solid culture, the liquid cultivating method to aforementioned routine carries out improved cultural method.
Described nutrition base is:
A, grain (as rice, broomcorn millet, millet, wheat, bean, corn etc.) seed are or/and its by product; Perhaps
The branch of B, plant, leaf, root, flower or fruit, perhaps its mixture; Perhaps
The skin of C, animal, hair or meat, perhaps its mixture; Perhaps
Two kinds combination among three kinds of A, B, the C or several combination.
As required, can in the nutrition base, add mineral matter.
The proportioning of mould fungal spore agent of wood and nutrition base is:
A, when the mould fungal spore agent drying of solid wood through cultivating gained is crushed into conidial powder, the percentage by weight of conidial powder is 25%～75%, all the other are the nutrition base of drying pulverizing;
B, when the mould fungal spore agent of liquid wood through cultivating gained is spore liquid, the percentage by weight of spore liquid is 30%～70%, all the other are the nutrition base of drying pulverizing;
C, when conidial powder and spore liquid are mixed into wooden mould fungal spore agent, the percentage by weight of conidial powder and spore liquid is 25%～75%, all the other are the nutrition base that drying is pulverized.
But spore liquid also can not elimination moisture than preceding elimination moisture with the nutrition basigamy.
The granularity of the nutrition base that drying is pulverized is below the 5mm.
The application of the mould field proliferating agent of the present invention's wood:
Soil to land for growing field crops, booth, greenhouse, cultivated plant such as potted plant is poured water in advance, is sprayed water or sprinkles water, and makes ground moistening, then to the wooden mould field proliferating agent of damp soil surface dispensing;
Before seed or the seedling sowing, wooden mould field proliferating agent is evenly spread fertilizer over the fields on the damp soil surface;
Also with last same, wooden mould field proliferating agent is evenly spread fertilizer over the fields on the damp soil surface behind the planting seed;
Seedling after planting or the plant growth stage, wooden mould field proliferating agent is evenly spread fertilizer over the fields damp soil surface between seedling, is not sprinkling upon the blade face, also directly is not sprinkling upon the foundation place.
The mould field proliferating agent of wood is sprinkling upon the blade face can be influenced it and expand numerous effect, is sprinkling upon the foundation place crop of can burning in a large number.
Spreading fertilizer over the fields wooden mould field proliferating agent should carry out in the afternoon.
22.5 kilograms～150 kilograms of the mould field proliferating agent per hectare consumptions of wood.
It mainly is the prevention plant disease that the mould field proliferating agent of wood is used, and wooden mould fungi can not be cured the plant that has fallen ill.There is diseased plant to find as the field, illustrate that the disease fungi infects part or most crop, should or not use wooden mould this moment with the chemical pesticide sterilization of interior absorption, otherwise not only affect control adversely, also can cause wooden mould fungi not have the erroneous picture of effect to the user.
The mechanism of action of the mould fungi prevention of wood plant disease belongs to interbiotic antagonism, mainly shows as sterilization, bacteriolyze, competition and hyperparasitism.Use the resistance that wooden mould fungi can also be induced crop.
The advantage of the mould field proliferating agent of wood:
One, former wooden mould fungi application process are to mix native hundreds of times with conidial powder to spread fertilizer over the fields or admix plow layer, plant plant hole with the centesimal ratio of number, and such application process recruitment is too big, and practical application is had any problem.
The mould field proliferating agent of wood is as long as spread fertilizer over the fields to moistening plow layer surface, and method is simple, allows the user accept easily.
Two, former amount of application is excessive, and by plow layer, plant plant hole and mix and excute a law, every mu of the consumption of the mould pulvis of solid wood is more than double centner, and cost is higher.
The mould field proliferating agent of wood only needs with 1.5 kilograms～10.0 kilograms for every mu, and cost alleviates tens of times.
It three is effect aspects, the mould fungi of wood mixes soil spreads fertilizer over the fields, directly admixes plow layer, the application process of planting plant hole is subjected to the antibacterial factor in the soil influence, sprays the influence that is subjected to weather and environment with conidial powder, wooden mould spore germination, mycelial growth and grow all undesirable surely.And, the part inactivation is arranged in the transportation of commodity preparation with in storing because the spore of wooden mould fungi becomes current shorter, and under the situation that the spore amount of living reduces, the spore germination after above-mentioned application process influence is used, mycelial growth and growing surely;
The advantage of the mould field proliferating agent of wood is: carry the nutrition base.The advantage that carries the nutrition base has two aspects.The first, supply with wooden mould fungi with abundant nutrition make its mycelia fast, raised growth, grow in soil surely, and produce a large amount of conidiums, make the spore amount of supplying with the field can enlarge tens of times to hundreds of times.It two is, when the mould field proliferating agent of wood has under the situation of part spore inactivation in transportation with in storing, as long as the nutrition base that has the small part spore germination just can rely on to carry is quick, raised growth, product spore.When the mould field proliferating agent of wood is sprinkling upon on the wetland, the very fast moisture absorption of wooden mould field proliferating agent, under suitable temperature, 6 hours endospores are sprouted, mycelial growth, just can grow into the cotton-shaped bacterium colony of white cotton, 24 hours flocculence bacterium colony greening product spores in 12 hours.The mould field proliferating agent of wood was spread fertilizer over the fields in one week of back, and every gram can produce conidium more than 1,000,000,000.These fresh and alive spores are with flow air between crop, are diffused into each corner of soil surface and stem of plant, leaf, flower, really, sprout in suitable place, growth and growing surely.
In the said process, the wooden mould hypha,hyphae of the cotton-shaped bacterium colony of white cotton bottom can be grown in soil fast because of obtaining the abundant nutrition of nutrition base, grows in soil surely.
The mould field proliferating agent dispensing of wood can obtain sufficient oxygen, and not be subjected to the influence of the antibacterial factor in the soil on the damp soil surface, and growth rate is than fast several times in soil, and this is the another big characteristics of this product.
The mechanism of this product prevention crop root, basal part of stem fungal disease is, the mould field proliferating agent dispensing of wood is grown fast, is bred behind the damp soil surface and grows surely in soil, suppress the sprouting of disease fungal spore in the soil and the growth of disease hypha,hyphae, inhibition disease fungi is infected crop, reaches the purpose of prevention crop root, the generation of basal part of stem fungal disease.
This product prevention stem of plant, leaf, flower if really the mechanism of fungus diseases be, Trichoderma damp soil superficial growth, breeding at first suppress soil surface may damage to crops the sprouting of disease fungal spore of stem, leaf, flower, fruit and the growth of mycelia, reduce the dynamics that infects of disease fungi.Trichoderma produces a large amount of spores and discharges, disperses in the field in damp soil superficial growth, reproductive process, the part spore can land may be played a protective role by the position that the disease fungi encroaches in stem of plant, leaf, flower, fruit; Trichoderma can discharge a large amount of volatility antibiotic in growth, reproductive process, can suppress soil surface and stem of plant, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
The effect of using wooden mould field proliferating agent prevention root of the crop, basic stem fungal disease is sustainable more than two months; Prevention stem of plant, leaf, flower, fruit position fungal disease effect are sustainable more than 25 days.
This product uses simply, cost is low, protection effect is obvious, and non agricultural chemical residuum, is specially adapted to the pollution-less agriculture field.
The mould field proliferating agent diseases prevention example of wood:
The prevention of example one, vegetables, grey mould fruit rot of strawberry
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum two strain bacterial strains (numbering T25 and T96 certainly).
The prescription of conidial powder medium: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 600 grams, rapeseed cake 380 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 5mm.
The weight proportion of conidial powder and nutrition base is 25: 75, is 120 kilograms of per hectares at the morbidity season crop premorbid amount of application first time, and executing the applied once amount again in 1 month at interval is 45 kilograms of per hectares, and this example is executed secondary altogether, and protection effect reaches 91%.
The prevention of example two, cotton seedling blight (claiming the cotton seedling blight again)
The bacterial classification that this example uses is fungus Trichoderma Trichoderma harzianum one a strain bacterial classification (numbering T96 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 380 grams, corn flour 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 380 grams, rice meal 100 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.5mm.
The weight proportion of conidial powder and nutrition base is 30: 70, and per hectare is executed 45 kilograms before seedling is sowed, and executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 93%.
The prevention of example three, peanut damping off
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum one strain bacterial classification (numbering T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, corn stalk powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 35: 65, uses before sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 95%.
The prevention of example four, pimento and Phytophthora capsici disease
The bacterial classification that this example uses is fungus Trichoderma Trichoderma viride one a strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 400 grams, assorted leaf powder 100 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder and nutrition base is 40: 60, uses after sowing, and per hectare is executed 45 kilograms, executes 30 kilograms of per hectares again in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 90%.
The prevention of example five, jasmine southern blight
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 580 grams, rapeseed cake 400 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 2.0mm.
The weight proportion of conidial powder, spore liquid and nutrition base is 25: 20: 55, and 45 kilograms of per hectare consumptions are used at morbidity season crop premorbid, executes once in 2 months at interval, and this example is executed secondary altogether, and protection effect reaches 94%.
The prevention of example six, orchid fusarium wilt (claiming stem rot again)
The bacterial classification that this example uses is the mixed bacteria of fungus Trichoderma Trichoderma harzianum two strain bacterial classifications (numbering T25, T96 certainly) and Trichoderma viride one strain bacterial classification (numbering T32 certainly).
The prescription of conidial powder medium: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams.The prescription of spore liquid medium: potato 200 grams, sucrose 20 grams, potassium dihydrogen phosphate 1.5 grams, magnesium sulfate 0.5 gram add 1000 milliliters in water.The nutrition based formulas: wheat bran 500 grams, rapeseed cake 430 grams, corn flour 50 grams, meat and bone meal 20 grams, potassium dihydrogen phosphate 1.0 grams, potassium nitrate 1.0 grams, sodium tetraborate 1.0 grams, the granularity of nutrition base is below the 3.0mm.
The weight proportion of conidial powder, spore liquid and nutrition base is 50: 20: 30, and 60 kilograms of per hectare consumptions are used at morbidity season crop premorbid, executes once in 45 days at interval, and this example is Shi Sici altogether, and protection effect reaches 96%.
In above-mentioned each example, conidial powder, spore liquid mixing formula can reach better protection effect; And the percentage by weight of the mould fungal spore agent of described wood when being 40%-60% protection effect better.
Remarks:
Expand numerous, i.e. expanding propagation; Field proliferating is promptly carried out expanding propagation in the field.
The expansion of wooden mould fungi in the past is numerous all to be finished in factory or laboratory, and the wooden mould fungal spore preparation that people will expand after numerous directly discharges the field, and to reach the purpose of preventing and treating crop pest, its application process mainly contains two kinds:
One is the mould fungal spore preparation of wood is mixed that soil spreads fertilizer over the fields or directly to admix soil or admix kind of a plant hole, makes wooden mould fungal spore sprout in soil, grow and grow in soil surely.
Its two, be with the mould fungal spore preparation of wood with dusting or converting water sprays, the mould fungal spore of wood is sprayed directly on to stem, leaf, flower, the fruit position of plant, make that it is sprouted at these positions, growth and growing surely.
The mould field proliferating agent of wood then is to form with mould fungal spore of wood and nutrition base, will expand numerous dose of dispensing in the field, and wooden mould fungi grows, breeds in the field, enlarges the spore amount.The spore that these expanding propagation are come out can be diffused in the soil that does not spread spore growth and grow surely, reaches the purpose of comprehensive protection.The spore that expanding propagation is come out can discharge, disperse, and the part spore can land may be sprouted, grow, grow surely in stem of plant, leaf, flower, fruit by the position that the disease fungi encroaches on, and plays a protective role.The mould fungi of wood discharges a large amount of volatility antibiotic in field growing, reproductive process, can suppress soil surface and stem of plant, leaf, flower, the sprouting of fruit position disease fungal spore and the growth of mycelia, reaches the effect of diseases prevention.
Claims (8)
1. wooden mould field proliferating agent, it is characterized in that evenly being mixed by mould fungal spore agent of wood and nutrition base and form, the mould fungal spore agent of described wood is the solid or liquid or solid and the mixtures of liquids that contain the mould fungal spore of a large amount of wood and a small amount of viable bacteria filament to the mould fungal culture gained of wood; The proportioning of mould fungal spore agent of described wood and nutrition base is:
A, when the mould fungal spore agent drying of solid wood through cultivating gained is crushed into conidial powder, the percentage by weight of conidial powder is 25%～75%, all the other are the nutrition base of drying pulverizing;
B, when the mould fungal spore agent of liquid wood through cultivating gained is spore liquid, the percentage by weight of spore liquid is 30%～70%, all the other are the nutrition base of drying pulverizing;
C, when conidial powder and spore liquid are mixed into wooden mould fungal spore agent, the percentage by weight of conidial powder and spore liquid is 25%～75%, all the other are the nutrition base that drying is pulverized.
2. the mould field proliferating agent of wood according to claim 1, it is characterized in that the mould fungi of described wood be in the fungus Trichoderma one or both combination or several combination.
3. the mould field proliferating agent of wood according to claim 1 is characterized in that the mould fungal spore of described wood is conidium or chlamydospore or conidium and chlamydosporic combination.
4. the mould field proliferating agent of wood according to claim 1, it is characterized in that the mould fungal spore of described wood be with following material as medium to the mould fungal culture gained of wood:
A, foodstuff seed are or/and its by product; Perhaps
The branch of B, plant, leaf, root, flower or fruit, perhaps its mixture; Perhaps
The skin of C, animal, hair or meat, perhaps its mixture; Perhaps
Two kinds combination among three kinds of A, B, the C or several combination.
5. the mould field proliferating agent of wood according to claim 4 is characterized in that adding mineral matter in the described medium.
6. the mould field proliferating agent of wood according to claim 1, the described nutrition base of its feature is:
A, foodstuff seed are or/and its by product; Perhaps
The branch of B, plant, leaf, root, flower or fruit, perhaps its mixture; Perhaps
The skin of C, animal, hair or meat, perhaps its mixture; Perhaps
Two kinds combination among three kinds of A, B, the C or several combination.
7. the mould field proliferating agent of wood according to claim 6 is added mineral matter in the described nutrition base of its feature.
8. the mould field proliferating agent of wood according to claim 1, it is characterized in that described spore liquid mix with the nutrition base before elimination moisture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009100958768A CN101485337B (en) | 2009-02-16 | 2009-02-16 | Agent for propagating Trichoderma sp. in field |
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100958768A CN101485337B (en) | 2009-02-16 | 2009-02-16 | Agent for propagating Trichoderma sp. in field |
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| CN101485337A CN101485337A (en) | 2009-07-22 |
| CN101485337B true CN101485337B (en) | 2011-11-30 |
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| CN2009100958768A Expired - Fee Related CN101485337B (en) | 2009-02-16 | 2009-02-16 | Agent for propagating Trichoderma sp. in field |
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| CN103320330B (en) * | 2012-12-25 | 2015-02-04 | 山东省农业科学院农产品研究所 | Trichoderma aureoviride strain and application thereof |
| CN112760236B (en) * | 2021-02-24 | 2023-05-16 | 云南省烟草公司普洱市公司 | Biocontrol strain for antagonizing phytophthora nicotianae and field propagation method and application of biocontrol strain and microbial inoculum thereof |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337164A (en) * | 2000-08-09 | 2002-02-27 | 玉溪红塔烟草(集团)有限责任公司 | Microbial bactericide and preparation method thereof |
| CN1473475A (en) * | 2003-05-21 | 2004-02-11 | 云南大学 | Method for increasing growing and propagating ability of fungus agent in soil |
| CN101028006A (en) * | 2006-07-31 | 2007-09-05 | 云南生物制药有限公司 | Production of trichodermin agricultural chemicals |
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2009
- 2009-02-16 CN CN2009100958768A patent/CN101485337B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1337164A (en) * | 2000-08-09 | 2002-02-27 | 玉溪红塔烟草(集团)有限责任公司 | Microbial bactericide and preparation method thereof |
| CN1473475A (en) * | 2003-05-21 | 2004-02-11 | 云南大学 | Method for increasing growing and propagating ability of fungus agent in soil |
| CN101028006A (en) * | 2006-07-31 | 2007-09-05 | 云南生物制药有限公司 | Production of trichodermin agricultural chemicals |
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| CN101485337A (en) | 2009-07-22 |
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