RU2291620C1 - Method for plant growth stimulation and protection from diseases - Google Patents

Abstract

FIELD: agriculture, in particular plant protection from diseases.

SUBSTANCE: seeds are treated before seeding with composition containing bacterial cells of microorganism belonging to genus Pseudomonas in form of complex based on three Pseudomonas Species strains in amount of 2.3-2.7 ml per 1 ton of seeds, and physiologically active components, such as humin fertilizer. Said composition is diluted before seed treatment in cultural liquid of 3-day Chlorella Vulgaris.

EFFECT: effective plant growth stimulation; decreased plant affecting by pathogen microorganisms.

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Description

The invention relates to agriculture and can be used to protect plants from phytopathogenic microorganisms.

A known method of stimulating the growth and protection of plants from diseases, including treating the seeds before sowing or planting a composition from a mixture of strains of bacterial cells, humates and trace elements [1].

The technical result is an increase in the efficiency of the method and crop yields.

The technical result is achieved in that in the known method, comprising treating the seeds with a composition of bacterial cells of a microorganism of the genus Pseudomonas in the form of a complex of strains of Pseudomonas Species 7G, Pseudomonas Species 7G 2K, Pseudomonas Species 17-2 and physiologically active components according to the invention as physiologically active components use the Darina humic fertilizer, the ratio of bacterial strains is 0.7: 1.5: 0.7-1.5: 0.7: 1.5, the concentration of bacterial cells is 2-5 · 10 ¹⁰ cells / ml, and the ratio the complex of strains and Darina is 1: 1-1: 1.8, comp The preparation is prepared before seed treatment by diluting in a culture fluid of the three-day Chlorella vulgaris, moreover, for processing 1 ton of seeds, 2.3-2.7 ml of a complex of bacteria strains are used, and the working solution consumption rate is 10-12 liters per ton of grain and leguminous crops and 70 -80 liters per ton of potato tubers.

Strains Pseudomonas Species 7G, Pseudomonas sp. 7G 2K and Pseudomonas sp. 17-2 are isolated from the rhizosphere population of microorganisms of wild cereal plants. These strains were deposited in the Research Institute “Collection of microorganism cultures” of the State Scientific Center of the World Bank “Vector” under registration numbers: 7G (Research Institute of KKM V-694); 7G 2K (NII KKM V-695) and 17-2 (NII KKM V-696).

Strains are non-pathogenic for warm-blooded animals and humans.

Strain Pseudomonas sp. 7G has a high antagonistic activity on phytopathogens Alternaria specias (1), Bipolarus sorokiniana (2), Helmintosporium sativum (3) and Fusarium oxysporum (4).

Strain Pseudomonas sp. 7G 2K has a high antagonistic activity on phytopathogens (1), (3) and (4), as well as Rhizoctonia solani, Phytophtora infestans.

Strain Pseudomonas sp. 17-2 - in relation to (1), (2), (4), as well as Fusarium sumbucinum.

The composition is prepared as follows. First, each strain is grown separately, for this they are seeded on a nutrient medium (LB broth). The culture is grown for 12 hours at 30 ° C and immediately use the obtained biomass as an inoculum for the fermenter. Fermentation is carried out at 30 ° C under aeration under a pressure of 0.04 kg / cm ² at a pH of 7.0-7.2. After 12-16 hours, the titer of the cell suspension reaches 2-5 · 10 ¹⁰ cells / ml.

Then, the obtained biomass of the strains is mixed in the required ratio with the Darina humic fertilizer and introduced into the container with the 3-day-old Chlorella Vulgaris culture fluid.

Example 1. The effectiveness of the method was studied to reduce the degree of damage to winter wheat cultivars Don-93 by root rot and yield.

Control wheat seeds before sowing were processed according to the prototype. Experimental wheat seeds were processed according to the proposed method, according to which the biomass of bacterial strains with a concentration of 2-5 · 10 ¹⁰ cells / ml was mixed in the ratio of Pseudomonas sp. 7G: Pseudomonas sp. 7G 2K: Pseudomonas sp. 17-2 0.7: 1.5: 0.7. A mixture of 2.5 ml of the strain complex and 2.5 ml of Darina (1: 1 ratio) was diluted with Chlorella Vulgaris three-day culture fluid in 10-12 liters of fluid.

The resulting working solution was used to treat 1 ton of wheat seeds.

The results are presented in table 1.

Example 2. The effectiveness of the method was studied for yield and reducing the degree of damage to soybean varieties VNIIOZ-86 fusarium. In the experiment, the biomass of the strains with a concentration of 2-5 · 10 ¹⁰ cells / ml was mixed in the ratio 7G: 7G 2K: 17-2 0.7: 0.7: 1.5.

A mixture of 2.3 ml of the strain complex and 2.8 ml of Darina (1: 1.2 ratio) was diluted with Chlorella Vulgaris three-day culture fluid in 10-12 liters of fluid. The resulting working solution was used to process 1 ton of soybean seeds. In the control, the seeds were processed according to the prototype.

The results are presented in table 2.

Example 3. The effectiveness of the method was studied for yield and reducing the degree of damage to the hybrid corn of ROSS 331 MV fungal and bacterial diseases. In the experiment, the biomass of the strains with a concentration of 2-5 · 10 ¹⁰ cells / ml was mixed in the ratio of 7G: 7G 2K: 17: 2 0.7: 1.5: 0.7. A mixture of 2.5 ml of the strain complex and 2.5 ml of Darina (1: 1 ratio) was diluted with Chlorella Vulgaris three-day culture fluid in 10-12 liters of fluid. The resulting solution was used to process one ton of seeds.

In the control, the seeds were processed according to the prototype.

The results are presented in table 3.

Example 4. The effectiveness of the method was studied to reduce damage to potatoes of the Romano variety by diseases caused by phytopathogenic fungi of the genus Phytophora and Rizoctonia.

In the experiment, the biomass of the strains with a concentration of 2-5 · 10 ¹⁰ cells / ml was mixed in the ratio of 7G: 7G 2K: 17-2 1,5: 0,7: 1,5.

A mixture of 2.7 ml of the strain complex and 4.86 ml of Darina (1: 1.8 ratio) was diluted with Chlorella Vulgaris three-day culture fluid in 70-80 liters of fluid. The resulting working solution was used to process 1 ton of tubers. In the control, the tubers were processed according to the prototype.

The results are presented in table 4.

Field studies were carried out in the OPH Irrigated GNU VNIIOZ.

Table 1
The influence of the method of presowing treatment of seeds of winter wheat varieties Don-93 on the defeat of root rot and yield Experience option The defeat of wheat plants by root rot,% Productivity, t / ha Prototype Processing - Control 3,7 3,1 The processing of the proposed method 2,5 3,5 table 2
The influence of the method of pre-sowing seed treatment of soybean varieties VNIIOZ-86 on the defeat of plant seedlings by Fusarium infection and yield Experience option The defeat of plant seedlings by Fusarium infection,% Productivity, t / ha Prototype seed treatment - control 12.6 1.81 The processing of the proposed method 3,1 2.23

Table 3
The effect of the presowing treatment of seeds of the corn hybrid ROSS 331 MB on the defeat of plants with fungal and bacterial diseases and the yield of grain and green mass Experience option The defeat of corn plants,% Grain productivity, t / ha Green mass productivity, t / ha Prototype seed treatment - control 12 5.5 51.8 Seed treatment according to the proposed method 5 7.8 73.0 Table 4
The influence of the method of pre-planting treatment of potato tubers on productivity Experience option Harvest potatoes, c / ha Increase, c / ha / (%) Tuber processing according to the prototype - control 187.5-195.0 - The processing of tubers by the proposed method 220-240 32.5-45 / 17% -23%

Chlorella Vulgaris culture fluid contains enzymes, hormones, vitamins, trace elements, amino acids and the antibiotic chlorellin. The latter, together with a complex of strains, shows synergism, and the whole composition as a whole and the method of seed treatment provides a more pronounced antagonistic effect on phytopathogens.

The use of culture fluid three-day Chlorella Vulgaris due to obtaining maximum efficiency.

Thus, the proposed method reduces the degree of damage to plants agricultural crops phytopathogenic microorganisms and increases productivity compared with the prototype.

In addition, the proposed method provides a more effective stimulation of plant growth, as evidenced by the appearance of seedlings after sowing seeds earlier than 1 day in the experiment with winter wheat; for 2 days in the experiment with soy and for 3 days in the experiment with potatoes compared to the prototype.

Information sources

1. RU 2130264, A 01 N 63/00, publ. 05/20/99.

Claims (1)

A method of stimulating growth and protecting plants from diseases, comprising treating the seeds with a composition of bacterial cells of a microorganism of the genus Pseudomonas in the form of a complex of strains of Pseudomonas Species 7G, Pseudomonas Species 7G 2K, Pseudomonas Species 17-2 and physiologically active components, characterized in that as physiologically active the components use the Darina humic fertilizer, the ratio of bacterial strains is 0.7: 1.5: 0.7-1.5: 0.7: 1.5, the concentration of bacterial cells is 2-5 · 10 ¹⁰ cells / ml, the ratio of the complex of strains and "Darina" is 1: 1-1: 1.8, preparing the composition t before seed treatment by diluting in a culture fluid of three-day Chlorella Vulgaris, moreover, for processing 1 ton of seeds, 2.3-2.7 ml of a complex of bacteria strains are used, and the working solution consumption rate is 10-12 l per ton of grain and leguminous crops and 70- 80 liters per ton of potato tubers.