CN105061068A - Moringa oleifera polysaccharide-rich biological liquid fertilizer and preparation method thereof - Google Patents
Moringa oleifera polysaccharide-rich biological liquid fertilizer and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a moringa oleifera polysaccharide-rich biological liquid fertilizer and a preparation method thereof. The fertilizer comprises the following components in parts by weight: 5-15 parts of moringa oleifera polysaccharide, 10-25 parts of gamma-polyglutamic acid, 3-8 parts of attapulgite, 3-8 parts of a moringa oleifera protein nutrient solution, 10-20 parts of bacillus megatherium liquid, 5-15 parts of bacillus subtilis liquid, 5-15 parts of bacillus licheniformis liquid, 5-15 parts of actinomycetes liquid, 5-15 parts of lactobacillus plantarum liquid and 5-15 parts of photosynthetic bacterium liquid. According to the preparation method, liquid fermentation is carried out on moringa oleifera polysaccharide, gamma-polyglutamic acid and the other components to obtain the fertilizer. According to the moringa oleifera polysaccharide-rich biological liquid fertilizer, the probiotic components and other organic nutrient components contained in the fertilizer can give play to an obvious synergistic effect, and compared with a separate probiotic component fertilizer and a separate organic nutrient component fertilizer, the moringa oleifera polysaccharide-rich biological liquid fertilizer can promote the yield of crops to be increased by around 15% and 25% respectively; besides, the using method is various, root and seedling strengthening of crops can be quickly promoted, the quality of crops is improved, and compared with an ordinary fertilizer, the fertilizer can promote the yield of agricultural products to be increased by around 20%.
Description
Technical field
The present invention relates to medical art, be specifically related to a kind of Moringa polysaccharide enriched biological liquid fertilizer and preparation method thereof.
Background technology
Life-time service due to chemical fertilizer can damage the crumb structure of the suitable Bacterial community of soil and soil, so in order to the ecology of soil protection and soil fertility, the use of microbial fertilizer is more and more extensive.But existing liquid microbe fertilizer is monistic biological probiotic bacterium fertilizer mostly in the market, as azotobacteria fertilizer, phosphorus bacteria and B. mucilaginocus etc., these fertilizer are used alone and only can meet the demand of crop to single nutrition, and cost is high, consumption is large, as accomplished that combination rationally with the use of being difficult to, proportioning science, thus can not play the fertilizer efficiency of fertilizer well.
A kind of good bio-feritlizer has strict requirement to aspects such as living bacteria count, miscellaneous bacteria rate, shelf lifes, but the poor management lattice at present to bio-feritlizer, even occur without production licence, production technique falls behind, effective bacterial content is low, miscellaneous bacteria exceeds standard phenomenon, some producer is in order to improve the N-P-K content in fertilizer, with the addition of chemical fertilizer or the additive of underexercise, cause effective bacterium dead, lose the effect of bio-fertilizer, bring loss to agriculture production.
Summary of the invention
For solving the problem, the object of this invention is to provide a kind of Moringa polysaccharide enriched biological liquid fertilizer and preparation method thereof.
The present invention for achieving the above object, is achieved through the following technical solutions:
A kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 5 ~ 15 parts, gamma-polyglutamic acid-10 ~ 25 parts, attapulgite 3 ~ 8 parts, Moringa nutritious protein liquid 3 ~ 8 parts, bacillus megaterium liquid 10 ~ 20 parts, subtilis liquid 5 ~ 15 parts, Bacillus licheniformis liquid 5 ~ 15 parts, actinomycetes liquid 5 ~ 15 parts, plant lactobacillus liquid 5 ~ 15 parts and photosynthetic bacteria liquid 5 ~ 15 parts; Described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 80 ~ 100 orders, add water stirring and obtain slurries in 0.5 ~ 1 hour, the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
For realizing object of the present invention further, can also by the following technical solutions:
Preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, the viable count in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer is 8 ~ 1,200,000,000.
Preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, attapulgite 5 parts, Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts, subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts, described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 90 orders, add water to stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Further preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, the viable count in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer is 900,000,000.
The present invention also comprises a kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer, comprises the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 80 ~ 100 orders, add water stirring and obtain slurries in 0.5 ~ 1 hour, the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 5 ~ 15 parts, gamma-polyglutamic acid-10 ~ 25 parts, step is gained attapulgite fine powder 3 ~ 8 parts, step 1. gained Moringa nutritious protein liquid 3 ~ 8 parts, bacillus megaterium liquid 10 ~ 20 parts 2., subtilis liquid 5 ~ 15 parts, Bacillus licheniformis liquid 5 ~ 15 parts, actinomycetes liquid 5 ~ 15 parts, plant lactobacillus liquid 5 ~ 15 parts and photosynthetic bacteria liquid 5 ~ 15 parts carry out liquid fermenting and within 14 ~ 16 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 25 ~ 30 DEG C.
The preparation method of preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, comprises the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 90 orders, add water and stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, step is gained attapulgite fine powder 5 parts, step 1. gained Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts 2., subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts carry out liquid fermenting and within 15 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 28 DEG C.
The invention has the advantages that:
Moringa polysaccharide enriched biological liquid fertilizer formula combination of the present invention is scientific and reasonable, in every milliliter of fertilizer, living bacteria count can reach about 1,000,000,000, total nutrient amount reaches about 13%, and miscellaneous bacteria rate is low, this index is well below national normal value, not only contain the nutritive ingredient of mineral manure and organic fertilizer but also contain compound probiotic in this liquid fertilizer, all kinds of nutritive elements needed for crop growthing development can not only be provided, improve the water retention capacity of soil, the pH-value of balanced soil, and the compound probiotic wherein contained has obvious P and K decomposing function, and the multiple enzyme that its amount reproduction produces can reduce the toxin in organism sweetening of the soil, crop photosynthesis can be promoted, aerobic repiration, fixed nitrogen, Gu physiological functions such as carbon, biological control effect is played to harmful microorganism, strengthen the resistance of farm crop,
The probiotic composition contained in Moringa polysaccharide enriched biological liquid fertilizer of the present invention and other Organic nutrient can play obvious synergy, compare independent probiotics analysis fertilizer and independent Organic nutrient fertilizer can promote increasing crop yield 15% and about 25% respectively;
The using method of Moringa polysaccharide enriched biological liquid fertilizer of the present invention is various; foliage spray can be adopted, water root, soak seed and dip in the modes such as root and directly act on farm crop; the growth that plant hormone substance stimulates farm crop all can be produced in each period of crop; and absorb fast; the strong root strong sprout of crop can be promoted; flower and fruit protecting; promote that the hyperchromic increasing of fruit is sweet; improve the quality of farm crop; and have wide range of applications; adapt to multiple kinds of crops, compare common fertilizer and can promote that agricultural-food increase production about 20%.
Preparation method's flow process of Moringa polysaccharide enriched biological liquid fertilizer of the present invention is few, and technique is simply easily implemented, and is applicable to scale operation.
Embodiment
A kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 5 ~ 15 parts, gamma-polyglutamic acid-10 ~ 25 parts, attapulgite 3 ~ 8 parts, Moringa nutritious protein liquid 3 ~ 8 parts, bacillus megaterium liquid 10 ~ 20 parts, subtilis liquid 5 ~ 15 parts, Bacillus licheniformis liquid 5 ~ 15 parts, actinomycetes liquid 5 ~ 15 parts, plant lactobacillus liquid 5 ~ 15 parts and photosynthetic bacteria liquid 5 ~ 15 parts; Described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 80 ~ 100 orders, add water stirring and obtain slurries in 0.5 ~ 1 hour, the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
For realizing object of the present invention further, can also by the following technical solutions:
Preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, the viable count in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer is 8 ~ 1,200,000,000.
Preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, attapulgite 5 parts, Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts, subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts, described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 90 orders, add water to stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Further preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, the viable count in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer is 900,000,000.
The present invention also comprises a kind of preparation method of Moringa polysaccharide enriched biological liquid fertilizer, comprises the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 80 ~ 100 orders, add water stirring and obtain slurries in 0.5 ~ 1 hour, the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 5 ~ 15 parts, gamma-polyglutamic acid-10 ~ 25 parts, step is gained attapulgite fine powder 3 ~ 8 parts, step 1. gained Moringa nutritious protein liquid 3 ~ 8 parts, bacillus megaterium liquid 10 ~ 20 parts 2., subtilis liquid 5 ~ 15 parts, Bacillus licheniformis liquid 5 ~ 15 parts, actinomycetes liquid 5 ~ 15 parts, plant lactobacillus liquid 5 ~ 15 parts and photosynthetic bacteria liquid 5 ~ 15 parts carry out liquid fermenting and within 14 ~ 16 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 25 ~ 30 DEG C.
The preparation method of preferred a kind of Moringa polysaccharide enriched biological liquid fertilizer, comprises the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 90 orders, add water and stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, step is gained attapulgite fine powder 5 parts, step 1. gained Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts 2., subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts carry out liquid fermenting and within 15 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 28 DEG C.
Moringa polysaccharide of the present invention is by about leaf of Moringa crushed after being dried to 100 order, obtain leaf of Moringa powder, gained leaf of Moringa powder is mixed according to weight ratio 1: 3 ~ 5 with water, obtain mixed solution, filter after being refluxed 100 ~ 120 minutes at temperature 80 ~ 90 DEG C by mixed solution, filtrate obtains Moringa polysaccharide through underpressure distillation.
The present invention's bacillus megaterium liquid, subtilis liquid, Bacillus licheniformis liquid, actinomycetes liquid, plant lactobacillus liquid and photosynthetic bacteria liquid used is all cultivated according to the method for known technology and is obtained.
Embodiment 1
A kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 5 parts, gamma-polyglutamic acid-10 parts, attapulgite 3 parts, Moringa nutritious protein liquid 3 parts, bacillus megaterium liquid 10 parts, subtilis liquid 5 parts, Bacillus licheniformis liquid 5 parts, actinomycetes liquid 5 parts, plant lactobacillus liquid 5 parts and photosynthetic bacteria liquid 5 parts; Described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 80 orders, add water to stir and obtain slurries in 0.5 hour, the weight ratio of moringa seeds and water is 1: 3, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Embodiment 2
A kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 15 parts, gamma-polyglutamic acid-25 parts, attapulgite 8 parts, Moringa nutritious protein liquid 8 parts, bacillus megaterium liquid 20 parts, subtilis liquid 15 parts, Bacillus licheniformis liquid 15 parts, actinomycetes liquid 15 parts, plant lactobacillus liquid 15 parts and photosynthetic bacteria liquid 15 parts; Described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 100 orders, add water to stir and obtain slurries in 1 hour, the weight ratio of moringa seeds and water is 1: 5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Embodiment 3
A kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 12 parts, gamma-polyglutamic acid-18 parts, attapulgite 7 parts, Moringa nutritious protein liquid 4 parts, bacillus megaterium liquid 12 parts, subtilis liquid 8 parts, Bacillus licheniformis liquid 12 parts, actinomycetes liquid 9 parts, plant lactobacillus liquid 11 parts and photosynthetic bacteria liquid 7 parts; Described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 90 orders, add water to stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 3.5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Embodiment 4
A kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 8 parts, gamma-polyglutamic acid-25 parts, attapulgite 3 parts, Moringa nutritious protein liquid 4 parts, bacillus megaterium liquid 14 parts, subtilis liquid 6 parts, Bacillus licheniformis liquid 8 parts, actinomycetes liquid 12 parts, plant lactobacillus liquid 13 parts and photosynthetic bacteria liquid 10 parts; Described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 85 orders, add water to stir and obtain slurries in 50 minutes, the weight ratio of moringa seeds and water is 1: 4.5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Embodiment 5
A kind of Moringa polysaccharide enriched biological liquid fertilizer, with parts by weight, consist of the following composition: Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, attapulgite 5 parts, Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts, subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts, described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 90 orders, add water to stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Embodiment 6
By the preparation method of a kind of Moringa polysaccharide enriched biological liquid fertilizer of the quality proportioning gained of embodiment 1, comprise the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 80 orders, add water and stir and obtain slurries in 0.5 hour, the weight ratio of moringa seeds and water is 1: 3, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid.
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 5 parts, gamma-polyglutamic acid-10 parts, step is gained attapulgite fine powder 3 parts, step 1. gained Moringa nutritious protein liquid 3 parts, bacillus megaterium liquid 10 parts 2., subtilis liquid 5 parts, Bacillus licheniformis liquid 5 parts, actinomycetes liquid 5 parts, plant lactobacillus liquid 5 parts and photosynthetic bacteria liquid 5 parts carry out liquid fermenting and within 14 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 25 DEG C.
Embodiment 7
By the preparation method of a kind of Moringa polysaccharide enriched biological liquid fertilizer of the quality proportioning gained of embodiment 2, comprise the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 100 orders, add water and stir and obtain slurries in 1 hour, the weight ratio of moringa seeds and water is 1: 5, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 15 parts, gamma-polyglutamic acid-25 parts, step is gained attapulgite fine powder 8 parts, step 1. gained Moringa nutritious protein liquid 8 parts, bacillus megaterium liquid 20 parts 2., subtilis liquid 15 parts, Bacillus licheniformis liquid 15 parts, actinomycetes liquid 15 parts, plant lactobacillus liquid 15 parts and photosynthetic bacteria liquid 15 parts carry out liquid fermenting and within 16 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 30 DEG C.
Embodiment 8
By the preparation method of a kind of Moringa polysaccharide enriched biological liquid fertilizer of the quality proportioning gained of embodiment 3, comprise the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 90 orders, add water and stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 3.5, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 12 parts, gamma-polyglutamic acid-18 parts, step is gained attapulgite fine powder 7 parts, step 1. gained Moringa nutritious protein liquid 4 parts, bacillus megaterium liquid 12 parts 2., subtilis liquid 8 parts, Bacillus licheniformis liquid 12 parts, actinomycetes liquid 9 parts, plant lactobacillus liquid 11 parts and photosynthetic bacteria liquid 7 parts carry out liquid fermenting and within 15 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 28 DEG C.
Embodiment 9
By the preparation method of a kind of Moringa polysaccharide enriched biological liquid fertilizer of the quality proportioning gained of embodiment 4, comprise the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 85 orders, add water and stir and obtain slurries in 50 minutes, the weight ratio of moringa seeds and water is 1: 4.5, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 8 parts, gamma-polyglutamic acid-25 parts, step is gained attapulgite fine powder 3 parts, step 1. gained Moringa nutritious protein liquid 4 parts, bacillus megaterium liquid 14 parts 2., subtilis liquid 16 parts, Bacillus licheniformis liquid 8 parts, actinomycetes liquid 12 parts, plant lactobacillus liquid 13 parts and photosynthetic bacteria liquid 10 parts carry out liquid fermenting and within 14 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 26 DEG C.
Embodiment 10
By the preparation method of a kind of Moringa polysaccharide enriched biological liquid fertilizer of the quality proportioning gained of embodiment 5, comprise the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 90 orders, add water and stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, step is gained attapulgite fine powder 5 parts, step 1. gained Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts 2., subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts carry out liquid fermenting and within 15 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 28 DEG C.
Comparative example 1
With parts by weight, by bacillus megaterium liquid 12 parts, subtilis liquid 8 parts, Bacillus licheniformis liquid 12 parts, actinomycetes liquid 9 parts, plant lactobacillus liquid 11 parts and photosynthetic bacteria liquid 7 parts mixing, obtain microbial liquid fertilizer.
Comparative example 2
With parts by weight, Moringa polysaccharide 12 parts, gamma-polyglutamic acid-18 parts, attapulgite 7 parts and Moringa nutritious protein liquid 4 parts of Homogeneous phase mixing, be crushed to more than 200 orders, obtain Moringa polysaccharide fertilizer, described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 90 orders, adds water and stirs and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 3.5, gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid.
Detect the Moringa polysaccharide enriched biological liquid fertilizer of embodiment 1 ~ 5, result as shown in Table 1 and Table 2.
The product technology of the Moringa polysaccharide enriched biological liquid fertilizer of table 1 embodiment 1 ~ 5 detects data
| Living bacteria count, hundred million/ml | Total nutrient, N+P 2O 5+KO 2% | Miscellaneous bacteria rate, % | pH | Validity period | |
| Embodiment 1 | 8 | 10 | ≤2 | 6.0 | At least one year |
| Embodiment 2 | 9 | 11 | ≤2 | 7.0 | At least one year |
| Embodiment 3 | 11 | 13 | ≤2 | 6.0 | At least one year |
| Embodiment 4 | 11 | 12 | ≤2 | 7.0 | At least one year |
| Embodiment 5 | 12 | 15 | ≤1 | 7.0 | At least one year |
The innoxious detection data of the product of the Moringa polysaccharide enriched biological liquid fertilizer of table 2 embodiment 1 ~ 5
| Embodiment 1 | Embodiment 2 | Embodiment 3 | Embodiment 4 | Embodiment 5 | |
| Excrement colibacillus group number, individual/ml | 5 | 6 | 5 | 5 | 3 |
| Induced worm egg death rate, % | 100 | 100 | 100 | 100 | 100 |
| Arsenic and compound (in As) thereof, mg/kg | Do not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Cadmium and compound (in Cd) thereof, mg/kg | Do not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Lead and compound (in Pb) thereof, mg/kg | Do not detect | Do not detect | Do not detect | Do not detect | Do not detect |
| Chromium and compound (in Cr), mg/kg | 10 | 10 | 10 | 10 | 10 |
| Mercury and mercuric compounds (in Hg), mg/kg | Do not detect | Do not detect | Do not detect | Do not detect | Do not detect |
The using method of this product and applicable crops
1. this product is suitable for all kinds of rhizome vegetable, Green leaf vegetable, melon and fruit, flowers, fruit tree and various food crop, as paddy rice, corn, wheat etc.
2. consumption
1. foliage spray: crop growth period, to bloom or the phase of bearing fruit to spray the face anyway on plant blade faces according to 1: 450 ~ 550 times of diluents, sprayed once every 15 days;
2. root is watered: according to after 1: 900 ~ 1100 dilutions, filling root is carried out to crop, the strong seedling of root of the crop can be made to strengthen, and then improve the output of crop;
3. soak seed: according to seed soaking after 1: 250 ~ 350 dilutions 8 ~ 12 hours, percentage of germination and the seedling rate of crop can be significantly improved, make seedling neat for strong sprout;
4. root is dipped in: the nursery stock transplanted will be needed, add fine earth according to 1: 450 ~ 550 diluents and size mixing and dip in root, can delay seedling in advance, raising surviving rate.
3. result of use
Experimental plot plant spring wheat 40 mu, be divided into 8 groups at random, often organize 5 mu, experimental group 1 ~ 5 uses the Moringa polysaccharide enriched biological liquid fertilizer of embodiment 1 ~ 5 gained by the following method; Control group 1 uses the microbial liquid fertilizer of comparative example 1 gained according to identical method; The main Moringa polysaccharide fertilizer using comparative example 2 gained at sedimentation value during grain filling stage of spring wheat of control group 2, every mu of usage quantity is 50 jin, and control group 3 uses common fertilizer to specifications.
Moringa polysaccharide enriched biological liquid fertilizer and microbial liquid fertilizer are to the using method of spring wheat: sow after soaking 10 hours to seed after 1: 300 dilution, and to spray the face anyway on plant blade face according to 1: 500 times of diluent in filling stage of spring wheat, sprayed once every 15 days, spray twice altogether.
Maize planting is continued in this plot after getting wheat, Moringa polysaccharide enriched biological liquid fertilizer and microbial liquid fertilizer are to the using method of corn: soak sowing after 10 hours according to after 1: 320 dilution to seed, and respectively spray the face anyway on plant blade face once in tri-leaf period of corn and fringe phase according to 1: 500 times of diluent.
Moringa polysaccharide fertilizer is in the using method of corn field: control group 2 mainly uses the Moringa polysaccharide fertilizer of comparative example 2 gained in the tri-leaf period of corn, every mu of usage quantity is 50 jin.
The output of wheat and maize is as shown in table 3.
The wheat of table 3 experimental group 1 ~ 5 and control group 1 ~ 2, corn yield table
| Wheat per mu yield/kg | Corn per mu yield/kg | |
| Experimental group 1 | 552 | 685 |
| Experimental group 2 | 554 | 690 |
| Experimental group 3 | 562 | 694 |
| Experimental group 4 | 565 | 698 |
| Experimental group 5 | 580 | 720 |
| Control group 1 | 480 | 600 |
| Control group 2 | 450 | 550 |
| Control group 3 | 475 | 590 |
As can be seen from Table 3, the per mu yield that the experimental group 1 ~ 5 using Moringa polysaccharide enriched biological liquid fertilizer of the present invention compares control group 1 wheat and maize using microbial liquid fertilizer increases by 17.2% and 16.2% respectively, the per mu yield that the experimental group 1 ~ 5 using Moringa polysaccharide enriched biological liquid fertilizer of the present invention compares control group 2 wheat and maize using Moringa polysaccharide fertilizer increases by 25.0% and 26.8% respectively, the per mu yield that the experimental group 1 ~ 5 using Moringa polysaccharide enriched biological liquid fertilizer of the present invention compares control group 3 wheat and maize using common fertilizer increases by 18.4% and 18.2% respectively, as can be seen here, it is more obvious that common fertilizer effect of increasing production compared by Moringa polysaccharide enriched biological liquid fertilizer of the present invention, and the probiotic composition contained in this fertilizer and other nutritive ingredients can play obvious synergy.
Claims (6)
1. a Moringa polysaccharide enriched biological liquid fertilizer, it is characterized in that: with parts by weight, consist of the following composition: Moringa polysaccharide 5 ~ 15 parts, gamma-polyglutamic acid-10 ~ 25 parts, attapulgite 3 ~ 8 parts, Moringa nutritious protein liquid 3 ~ 8 parts, bacillus megaterium liquid 10 ~ 20 parts, subtilis liquid 5 ~ 15 parts, Bacillus licheniformis liquid 5 ~ 15 parts, actinomycetes liquid 5 ~ 15 parts, plant lactobacillus liquid 5 ~ 15 parts and photosynthetic bacteria liquid 5 ~ 15 parts; Described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 80 ~ 100 orders, add water stirring and obtain slurries in 0.5 ~ 1 hour, the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
2. a kind of Moringa polysaccharide enriched biological liquid fertilizer according to claim 1, is characterized in that: the viable count in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer is 8 ~ 1,200,000,000.
3. a kind of Moringa polysaccharide enriched biological liquid fertilizer according to claim 1, it is characterized in that: with parts by weight, consist of the following composition: Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, attapulgite 5 parts, Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts, subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts, described Moringa nutritious protein liquid is obtained by following steps: moringa seeds is crushed to 90 orders, add water to stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid.
4. a kind of Moringa polysaccharide enriched biological liquid fertilizer according to claim 3, is characterized in that: the viable count in every milliliter of Moringa polysaccharide enriched biological liquid fertilizer is 900,000,000.
5. a preparation method for Moringa polysaccharide enriched biological liquid fertilizer as claimed in claim 1, is characterized in that: comprise the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 80 ~ 100 orders, add water stirring and obtain slurries in 0.5 ~ 1 hour, the weight ratio of moringa seeds and water is 1: 3 ~ 5, and gained slurries are carried out millipore filtration, collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 5 ~ 15 parts, gamma-polyglutamic acid-10 ~ 25 parts, step is gained attapulgite fine powder 3 ~ 8 parts, step 1. gained Moringa nutritious protein liquid 3 ~ 8 parts, bacillus megaterium liquid 10 ~ 20 parts 2., subtilis liquid 5 ~ 15 parts, Bacillus licheniformis liquid 5 ~ 15 parts, actinomycetes liquid 5 ~ 15 parts, plant lactobacillus liquid 5 ~ 15 parts and photosynthetic bacteria liquid 5 ~ 15 parts carry out liquid fermenting and within 14 ~ 16 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 25 ~ 30 DEG C.
6. a preparation method for Moringa polysaccharide enriched biological liquid fertilizer as claimed in claim 3, is characterized in that: comprise the following steps:
1. the preparation of Moringa nutritious protein liquid: moringa seeds is crushed to 90 orders, add water and stir and obtain slurries in 45 minutes, the weight ratio of moringa seeds and water is 1: 4, and gained slurries are carried out millipore filtration, and collect filtrate, gained filtrate is Moringa nutritious protein liquid;
2. attapulgite is crushed to more than 200 orders, obtains attapulgite fine powder;
3. with parts by weight, by Moringa polysaccharide 10 parts, gamma-polyglutamic acid-20 parts, step is gained attapulgite fine powder 5 parts, step 1. gained Moringa nutritious protein liquid 5 parts, bacillus megaterium liquid 15 parts 2., subtilis liquid 10 parts, Bacillus licheniformis liquid 10 parts, actinomycetes liquid 10 parts, plant lactobacillus liquid 10 parts and photosynthetic bacteria liquid 10 parts carry out liquid fermenting and within 15 days, obtain Moringa polysaccharide enriched biological liquid fertilizer at 28 DEG C.
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