CN117265021A - Treatment method for carrying out anaerobic digestion on high ammonia nitrogen waste through co-digestion - Google Patents
Treatment method for carrying out anaerobic digestion on high ammonia nitrogen waste through co-digestion Download PDFInfo
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- 230000029087 digestion Effects 0.000 title claims abstract description 43
- 238000000034 method Methods 0.000 title claims abstract description 36
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 title claims abstract description 35
- 239000002699 waste material Substances 0.000 title claims abstract description 7
- 150000004668 long chain fatty acids Chemical class 0.000 claims abstract description 16
- IJGRMHOSHXDMSA-UHFFFAOYSA-N nitrogen Substances N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims abstract description 11
- 239000000758 substrate Substances 0.000 claims abstract description 9
- 229910052757 nitrogen Inorganic materials 0.000 claims abstract description 7
- 239000010815 organic waste Substances 0.000 claims abstract description 6
- 239000002054 inoculum Substances 0.000 claims description 16
- 210000003608 fece Anatomy 0.000 claims description 10
- ZQPPMHVWECSIRJ-KTKRTIGZSA-N oleic acid Chemical compound CCCCCCCC\C=C/CCCCCCCC(O)=O ZQPPMHVWECSIRJ-KTKRTIGZSA-N 0.000 claims description 10
- WRIDQFICGBMAFQ-UHFFFAOYSA-N (E)-8-Octadecenoic acid Natural products CCCCCCCCCC=CCCCCCCC(O)=O WRIDQFICGBMAFQ-UHFFFAOYSA-N 0.000 claims description 9
- LQJBNNIYVWPHFW-UHFFFAOYSA-N 20:1omega9c fatty acid Natural products CCCCCCCCCCC=CCCCCCCCC(O)=O LQJBNNIYVWPHFW-UHFFFAOYSA-N 0.000 claims description 9
- QSBYPNXLFMSGKH-UHFFFAOYSA-N 9-Heptadecensaeure Natural products CCCCCCCC=CCCCCCCCC(O)=O QSBYPNXLFMSGKH-UHFFFAOYSA-N 0.000 claims description 9
- ZQPPMHVWECSIRJ-UHFFFAOYSA-N Oleic acid Natural products CCCCCCCCC=CCCCCCCCC(O)=O ZQPPMHVWECSIRJ-UHFFFAOYSA-N 0.000 claims description 9
- 239000005642 Oleic acid Substances 0.000 claims description 9
- QXJSBBXBKPUZAA-UHFFFAOYSA-N isooleic acid Natural products CCCCCCCC=CCCCCCCCCC(O)=O QXJSBBXBKPUZAA-UHFFFAOYSA-N 0.000 claims description 9
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 6
- 239000010871 livestock manure Substances 0.000 claims description 5
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 claims description 4
- 239000010802 sludge Substances 0.000 claims description 3
- 241000287828 Gallus gallus Species 0.000 claims description 2
- 235000021314 Palmitic acid Nutrition 0.000 claims description 2
- WQEPLUUGTLDZJY-UHFFFAOYSA-N n-Pentadecanoic acid Natural products CCCCCCCCCCCCCCC(O)=O WQEPLUUGTLDZJY-UHFFFAOYSA-N 0.000 claims description 2
- 235000021313 oleic acid Nutrition 0.000 claims description 2
- OYHQOLUKZRVURQ-HZJYTTRNSA-N Linoleic acid Chemical compound CCCCC\C=C/C\C=C/CCCCCCCC(O)=O OYHQOLUKZRVURQ-HZJYTTRNSA-N 0.000 claims 1
- 235000020778 linoleic acid Nutrition 0.000 claims 1
- OYHQOLUKZRVURQ-IXWMQOLASA-N linoleic acid Natural products CCCCC\C=C/C\C=C\CCCCCCCC(O)=O OYHQOLUKZRVURQ-IXWMQOLASA-N 0.000 claims 1
- 230000005764 inhibitory process Effects 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 4
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 22
- 239000007789 gas Substances 0.000 description 13
- 229910021529 ammonia Inorganic materials 0.000 description 11
- 238000004519 manufacturing process Methods 0.000 description 11
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 10
- 239000000243 solution Substances 0.000 description 10
- 210000004027 cell Anatomy 0.000 description 9
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 8
- 235000015097 nutrients Nutrition 0.000 description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 5
- 239000008103 glucose Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- XKRFYHLGVUSROY-UHFFFAOYSA-N Argon Chemical compound [Ar] XKRFYHLGVUSROY-UHFFFAOYSA-N 0.000 description 4
- 235000019270 ammonium chloride Nutrition 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000012528 membrane Substances 0.000 description 4
- 238000001179 sorption measurement Methods 0.000 description 4
- 239000003463 adsorbent Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 229910052567 struvite Inorganic materials 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- ALYNCZNDIQEVRV-UHFFFAOYSA-N 4-aminobenzoic acid Chemical compound NC1=CC=C(C(O)=O)C=C1 ALYNCZNDIQEVRV-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 description 2
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 2
- CKMXBZGNNVIXHC-UHFFFAOYSA-L ammonium magnesium phosphate hexahydrate Chemical compound [NH4+].O.O.O.O.O.O.[Mg+2].[O-]P([O-])([O-])=O CKMXBZGNNVIXHC-UHFFFAOYSA-L 0.000 description 2
- 229910052786 argon Inorganic materials 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 210000002390 cell membrane structure Anatomy 0.000 description 2
- RMRCNWBMXRMIRW-BYFNXCQMSA-M cyanocobalamin Chemical compound N#C[Co+]N([C@]1([H])[C@H](CC(N)=O)[C@]\2(CCC(=O)NC[C@H](C)OP(O)(=O)OC3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)C)C/2=C(C)\C([C@H](C/2(C)C)CCC(N)=O)=N\C\2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O RMRCNWBMXRMIRW-BYFNXCQMSA-M 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 238000000855 fermentation Methods 0.000 description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- QJGQUHMNIGDVPM-UHFFFAOYSA-N nitrogen group Chemical group [N] QJGQUHMNIGDVPM-UHFFFAOYSA-N 0.000 description 2
- GHOKWGTUZJEAQD-UHFFFAOYSA-N pantothenic acid Chemical compound OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 description 2
- 239000012466 permeate Substances 0.000 description 2
- 150000003904 phospholipids Chemical class 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- LXNHXLLTXMVWPM-UHFFFAOYSA-N pyridoxine Chemical compound CC1=NC=C(CO)C(CO)=C1O LXNHXLLTXMVWPM-UHFFFAOYSA-N 0.000 description 2
- 239000010865 sewage Substances 0.000 description 2
- 239000011550 stock solution Substances 0.000 description 2
- 239000002351 wastewater Substances 0.000 description 2
- JIAARYAFYJHUJI-UHFFFAOYSA-L zinc dichloride Chemical compound [Cl-].[Cl-].[Zn+2] JIAARYAFYJHUJI-UHFFFAOYSA-L 0.000 description 2
- OYHQOLUKZRVURQ-NTGFUMLPSA-N (9Z,12Z)-9,10,12,13-tetratritiooctadeca-9,12-dienoic acid Chemical compound C(CCCCCCC\C(=C(/C\C(=C(/CCCCC)\[3H])\[3H])\[3H])\[3H])(=O)O OYHQOLUKZRVURQ-NTGFUMLPSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 1
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- PLXBWHJQWKZRKG-UHFFFAOYSA-N Resazurin Chemical compound C1=CC(=O)C=C2OC3=CC(O)=CC=C3[N+]([O-])=C21 PLXBWHJQWKZRKG-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 238000005273 aeration Methods 0.000 description 1
- 229960004050 aminobenzoic acid Drugs 0.000 description 1
- MXZRMHIULZDAKC-UHFFFAOYSA-L ammonium magnesium phosphate Chemical compound [NH4+].[Mg+2].[O-]P([O-])([O-])=O MXZRMHIULZDAKC-UHFFFAOYSA-L 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 239000007640 basal medium Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- MPTQRFCYZCXJFQ-UHFFFAOYSA-L copper(II) chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Cu+2] MPTQRFCYZCXJFQ-UHFFFAOYSA-L 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 229960002104 cyanocobalamin Drugs 0.000 description 1
- 235000000639 cyanocobalamin Nutrition 0.000 description 1
- 239000011666 cyanocobalamin Substances 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 229940071106 ethylenediaminetetraacetate Drugs 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 229960000304 folic acid Drugs 0.000 description 1
- 235000019152 folic acid Nutrition 0.000 description 1
- 239000011724 folic acid Substances 0.000 description 1
- 238000004817 gas chromatography Methods 0.000 description 1
- 238000011065 in-situ storage Methods 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- AGBQKNBQESQNJD-UHFFFAOYSA-M lipoate Chemical compound [O-]C(=O)CCCCC1CCSS1 AGBQKNBQESQNJD-UHFFFAOYSA-M 0.000 description 1
- 235000019136 lipoic acid Nutrition 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 229910052749 magnesium Inorganic materials 0.000 description 1
- CNFDGXZLMLFIJV-UHFFFAOYSA-L manganese(II) chloride tetrahydrate Chemical compound O.O.O.O.[Cl-].[Cl-].[Mn+2] CNFDGXZLMLFIJV-UHFFFAOYSA-L 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 229960003512 nicotinic acid Drugs 0.000 description 1
- 235000001968 nicotinic acid Nutrition 0.000 description 1
- 239000011664 nicotinic acid Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 238000010979 pH adjustment Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000011253 protective coating Substances 0.000 description 1
- 235000008160 pyridoxine Nutrition 0.000 description 1
- 239000011677 pyridoxine Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229940082569 selenite Drugs 0.000 description 1
- MCAHWIHFGHIESP-UHFFFAOYSA-L selenite(2-) Chemical compound [O-][Se]([O-])=O MCAHWIHFGHIESP-UHFFFAOYSA-L 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 229960000344 thiamine hydrochloride Drugs 0.000 description 1
- 235000019190 thiamine hydrochloride Nutrition 0.000 description 1
- 239000011747 thiamine hydrochloride Substances 0.000 description 1
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 1
- 229960002663 thioctic acid Drugs 0.000 description 1
- 229910021654 trace metal Inorganic materials 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 229940011671 vitamin b6 Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 239000011592 zinc chloride Substances 0.000 description 1
- 235000005074 zinc chloride Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
- C12P5/023—Methane
Abstract
The invention belongs to the field of anaerobic digestion, and relates to a treatment method for performing anaerobic digestion on high ammonia nitrogen waste through co-digestion. The method is that long-chain fatty acid is added into an anaerobic digestion system with high-nitrogen-content organic waste as a substrate, so that the concentration of initial long-chain fatty acid in the anaerobic digestion system is 1-2.5g/L. The ammonia nitrogen inhibition phenomenon can occur when the high-nitrogen organic waste is subjected to anaerobic digestion, and the inhibition effect of the ammonia nitrogen on the anaerobic digestion process can be relieved by co-digestion with LCFA.
Description
Technical Field
The invention belongs to the field of anaerobic digestion, and relates to a treatment method for performing anaerobic digestion on high ammonia nitrogen waste through co-digestion.
Background
Anaerobic Digestion (AD) is an environmentally friendly method of organic waste treatment that can utilize renewable energy sources and can be used for livestock manure treatment, but with higher nitrogen content.
The high nitrogen-containing organic matter is converted into high ammonia nitrogen in the anaerobic digestion process, ammonia has two existing forms in the anaerobic system, and free NH 3 And ionic NH 4 + The method comprises the steps of carrying out a first treatment on the surface of the The free ammonia can directly permeate the cell membrane to diffuse into the cell interior to combine with protons, so that the pH change in the cell is caused, and the activity of the cell is influenced; the ionic state is combined with the phospholipid bilayer to destroy the cell membrane structure, i.e. ammonia inhibits the digestion reaction.
Aiming at the phenomenon of ammonia inhibition in anaerobic digestion, the method is mainly used for relieving the phenomenon of ammonia inhibition in anaerobic digestion by the following means: (1) The ammonia inhibition phenomenon of the anaerobic digestion system is relieved by adding water to the anaerobic system to dilute the substrate so as to reduce the ammonia nitrogen concentration in the system. However, the method requires a large amount of water, which increases the amount of wastewater in the fermentation system. (2) Temperature and pH adjustment, however, are difficult and costly to adjust and control in an anaerobic system, and are not an effective method. (3) The adsorbent and the adsorption material are added, and the function of the adsorbent mainly has high specific surface area, so that the fixation of microorganisms can be facilitated, and ammonia nitrogen in the adsorption system can be reduced to reduce the ammonia nitrogen concentration so as to reduce the influence of high-concentration ammonia nitrogen on anaerobic digestion. However, the adsorption capacity of the adsorbent to ammonia nitrogen is limited, new adsorption materials are required to be continuously replaced, the treatment is complicated, and the cost is high. (4) ammonia nitrogen in-situ removal; such as ammonia stripping, membrane technology, struvite precipitation technology. Ammonia stripping can remove ammonia nitrogen in biogas slurry by utilizing gas flow, and the method is simple and high in efficiency, but the treatment process also needs to control the temperature and the pH value, and air is continuously blown in. The membrane technology realizes substance separation by utilizing the selective permeability of the membrane, but the membrane has higher cost and is not suitable for large-scale utilization. In the struvite precipitation method, magnesium source and phosphorus source are added to combine with ammonia nitrogen in wastewater to form magnesium ammonium phosphate precipitate so as to reduce ammonia nitrogen concentration in the system, but chemical is also required to be added, the proportion is required to be controlled, and the precipitate formed in an anaerobic digestion system may influence the anaerobic digestion process.
Disclosure of Invention
The invention provides a treatment method for carrying out anaerobic digestion on high-ammonia nitrogen waste by co-digestion in order to solve the problem of ammonia nitrogen inhibition phenomenon of high-nitrogen organic matters in an anaerobic digestion system in the anaerobic digestion process.
In order to achieve the above purpose, the present invention adopts the following technical scheme:
a treatment method for carrying out anaerobic digestion on high ammonia nitrogen waste by co-digestion is characterized in that,
long-chain fatty acid is added into an anaerobic digestion system with high-nitrogen-content organic waste as a substrate, so that the concentration of the initial long-chain fatty acid in the anaerobic digestion system is 1-2.5g/L.
Preferably, the initial concentration of long chain fatty acid is 2.0g/L.
Preferably, the long-chain fatty acid is one or more of oleic acid, linoleic acid, palmitic acid and the like.
Preferably, the inoculum in the anaerobic digestion system has a TS content of 2% and a VS content of 50%; anaerobic sludge from a sewage plant was cultured in an anaerobic digester at 37 ℃.
Further preferably, the culture conditions of the inoculum are: 0.6g/L of citric acid was added every three days and stirred every 2 hours for 10 minutes.
Preferably, the inoculum is present in the anaerobic digestion system in an amount of 20% by volume.
Preferably, the nitrogen content of the substrate is 1-6%, and the substrate can be chicken manure, pig manure and the like.
Preferably, anaerobic digestion is suitable with a C/N ratio of around 25:1.
Preferably, the temperature of the digestion treatment is 37.+ -. 0.5 ℃.
The invention has the beneficial effects that:
the ammonia nitrogen inhibition phenomenon can occur when the high-nitrogen organic waste is subjected to anaerobic digestion, and the inhibition effect of the ammonia nitrogen on the anaerobic digestion process can be relieved by co-digestion with LCFA.
Drawings
FIG. 1 is a graph of cumulative methane yield from anaerobic fermentation experiments under different conditions.
Detailed Description
The technical scheme of the invention is further described below by referring to the accompanying drawings, namely embodiments.
Experimental materials:
to illustrate the effectiveness of the invention, embodiments of the present invention simulate the anaerobic digestion system of high nitrogen containing substrates with cow dung and ammonium chloride.
Basal medium was prepared from the following stock solutions (the chemicals given below are concentrations in distilled water in g/l).
And (3) solution A: NH (NH) 4 Cl,100; sodium chloride, 10; mgCl 2 ·6H 2 O，10；CaCl 2 ·2H 2 O，5；
Liquid B, K 2 HPO 4 ·3H 2 O，200；
And C, liquid: 0.5 of resazurin;
and D, liquid: feCl as trace metal and selenite solution 2 ·4H 2 O，2；H 3 BO 3 0.05; zinc chloride, 0.05; copper chloride dihydrate, 0.038; manganese chloride tetrahydrate, 0.05; (NH) 4 ) 6 Mo 7 O 24 ·4H 2 O，0.05；AlCl 3 ，0.05；CoCl 2 ·6H 2 O，0.05；NiCl 2 ·6H 2 O,0.092; ethylenediamine tetraacetate, 0.5; concentrated hydrochloric acid, 1 ml; na (Na) 2 SeO 3 ·5H 2 O，0.1；
E, liquid: vitamin mixture (ingredients given in mg/l) biotin, 2; folic acid, 2; pyridoxine, 10; rizefin, 5; thiamine hydrochloride, 5; cyanocobalamin, 0.1; nicotinic acid, 5; p-aminobenzoic acid, 5; lipoic acid, 5; DL-pantothenic acid.
The anaerobic nutrient solution is as follows: to 975 ml of distilled water, 10 ml of the following stock solution (a) was added; (B) 2 ml; (C) 1 ml; (D) 1 ml and (E) 1 ml.
Cow dung (TS (solid content) =20%, VS (organic content) =80%);
inoculum (ts=2%, vs=50%) was taken from anaerobic sludge from the peninsula sewage plant and cultured in laboratory medium temperature (37 ℃) anaerobic digester.
Oleic acid was purchased.
The experimental process comprises the following steps:
21 serum bottles with a total volume of 300ml were prepared, divided into seven groups, each group of three parallel experiments, designated control, N0, N1, N2, N3, N4, N5; the working volume was 200mL. A shaker at 37℃was used, 130rpm. Argon aeration was used to create an anaerobic environment.
Control group: only 40mL of inoculum was added; 160mL anaerobic nutrient solution (ammonia nitrogen concentration is not regulated, and gas production interference of the inoculum is counteracted);
n0 group: 40mL of inoculum and 12.5g of cow dung were added, the ammonia nitrogen concentration was not adjusted, and anaerobic nutrient solution was added to a working volume of 200mL.
N1: adding 40mL of inoculum and 12.5g of cow dung, adding 2.29g of ammonium chloride to adjust the ammonia nitrogen concentration to 3g/L, and adding anaerobic nutrient solution to 200mL;
n2: 40mL of inoculum and 12.5g of cow dung were added, 0.4g of oleic acid (0.00142 mol) was added, and 2.29g of ammonium chloride was added to adjust the ammonia nitrogen concentration to 3g/L. Adding anaerobic nutrient solution to 200mL working volume
And N3: 40mL of inoculum and 12.5g of cow dung were added, 0.77g (0.00426. Mol) of glucose was added, and 2.29g of ammonium chloride was added to adjust the ammonia nitrogen concentration to 3g/L. Anaerobic nutrient solution was added to 200mL.
N4: 40mL of inoculum was added, and 0.4g (0.00142 mol) of oleic acid was added, and the ammonia nitrogen concentration was not adjusted, and the anaerobic nutrient solution was added to 200mL.
N5: 40mL of inoculum was added, 0.77g (0.00426 mol) of glucose was added, ammonia nitrogen concentration was not adjusted, and anaerobic nutrient solution was added to 200mL.
After bottling, sealing, and flushing for five minutes by argon to form an anaerobic environment. All the bottles were placed in a gas-bath shaker at 120rpm and 37℃and the gas production was measured daily by water drainage and the gas composition was measured by gas chromatography.
The N3 minus N5 was used to remove glucose gas production interference, the gas production data was designated X1.
The N2 minus N4 was used to remove oleic acid gas production interference, the gas production data being X2.
The results of the gas production are shown in figure 1. The invention uses N3 minus N5 to remove the glucose gas production interference, because the anaerobic digestion of the high nitrogen system introduces an additional carbon source and also improves the anaerobic digestion process (the C/N ratio is changed), and the glucose has the function of counteracting the additional carbon source introduced by the addition of oleic acid in the system, so that the result is more accurate. The N2 minus N4 was used to remove oleic acid gas production interference, the gas production data being X2.
The methane yield of group N0 without ammonia nitrogen inhibition was found to be 486.27mL/gVS by the methane yield map. In the group with ammonia nitrogen inhibition, N1 had an accumulated methane yield of 162.98mL/g VS and X2 (i.e., oleic acid group, 433.62 mL/gVS) had a methane yield higher than X1 (no oleic acid added, 373.46 mL/gVS). And the later period of gas production stagnancy is shortened, which proves that the co-digestion of LCFAs can effectively relieve the inhibition of ammonia nitrogen under a high ammonia nitrogen system. The method shows that the addition of long-chain fatty acid in the anaerobic digestion system of high ammonia nitrogen organic matters can play a role in reducing ammonia nitrogen inhibition.
Ammonia exists in two forms in anaerobic systems, and free NH 3 And ionic NH 4 + The method comprises the steps of carrying out a first treatment on the surface of the The free ammonia can directly permeate the cell membrane to diffuse into the cell interior to combine with protons, so that the pH change in the cell is caused, and the activity of the cell is influenced; the ionic state is combined with the phospholipid bilayer to destroy the cell membrane structure. The reason why the addition of long-chain fatty acid can play a role in reducing ammonia nitrogen inhibition is hypothesized that the low degradation rate of LCFA (long-chain fatty acid) prevents accumulation of VFA (volatile fatty acid) at an early stage in the anaerobic digestion process; in addition, protective coating mechanisms may form around bacterial and archaeal cells, limiting ammonia diffusion into the cell or providing a locally lower pH in the vicinity of the cell.
Claims (10)
1. A treatment method for carrying out anaerobic digestion on high ammonia nitrogen waste by co-digestion is characterized in that,
long-chain fatty acid is added into an anaerobic digestion system with high-nitrogen-content organic waste as a substrate, so that the concentration of the initial long-chain fatty acid in the anaerobic digestion system is 1-2.5g/L.
2. The process of claim 1, wherein the initial concentration of long chain fatty acid is 2.0g/L.
3. The method according to claim 1, wherein the long-chain fatty acid is one or more of oleic acid, linoleic acid and palmitic acid.
4. The process of claim 1, wherein the inoculum in the anaerobic digestion system has a TS content of 2% and a VS content of 50%.
5. The process of claim 1, wherein the inoculum in the anaerobic digestion system is anaerobic sludge.
6. The method of claim 1, wherein the inoculum is cultured under the following conditions: 0.6g/L of citric acid was added every three days and stirred every 2 hours for 10 minutes.
7. The process of claim 1, wherein the inoculum is present in the anaerobic digestion system in an amount of 20% by volume.
8. The process of claim 1, wherein the substrate has a nitrogen content of 1-6%.
9. The method according to claim 1, wherein the substrate is one of chicken manure and pig manure.
10. The process of claim 1, wherein the anaerobic digestion process is carried out at a temperature of 37±0.5 ℃.
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