CN112960879A - Method for slowing down inhibition of palmitic acid on methane production of anaerobic digestion waste oil - Google Patents

Method for slowing down inhibition of palmitic acid on methane production of anaerobic digestion waste oil Download PDF

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Publication number
CN112960879A
CN112960879A CN202110272607.5A CN202110272607A CN112960879A CN 112960879 A CN112960879 A CN 112960879A CN 202110272607 A CN202110272607 A CN 202110272607A CN 112960879 A CN112960879 A CN 112960879A
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palmitic acid
anaerobic digestion
inhibition
gvs
waste oil
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CN202110272607.5A
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Chinese (zh)
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吴坤
何霞
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Guilin University of Technology
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Guilin University of Technology
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    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F11/00—Treatment of sludge; Devices therefor
    • C02F11/02—Biological treatment
    • C02F11/04—Anaerobic treatment; Production of methane by such processes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
    • C12P5/02—Preparation of hydrocarbons or halogenated hydrocarbons acyclic
    • C12P5/023—Methane
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2101/00—Nature of the contaminant
    • C02F2101/30—Organic compounds
    • C02F2101/34—Organic compounds containing oxygen
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2209/00—Controlling or monitoring parameters in water treatment
    • C02F2209/28—CH4
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/30—Fuel from waste, e.g. synthetic alcohol or diesel

Abstract

The invention relates to a method for retarding the inhibition of palmitic acid on the methane production of anaerobic digestion waste oil, in particular to a method for retarding the inhibition of the palmitic acid on the methane production of the anaerobic digestion waste oil, which comprises the following steps of firstly mixing the palmitic acid with four metal ions (Ca)2+、Mg2+、Al3+、Fe3+) Saponifying to generate saponifiable matter, adding the saponifiable matter into an anaerobic digestion reactor, adding an inoculum to enable the ratio of the saponifiable matter to the inoculum to be 1(gVS/gVS), carrying out anaerobic digestion under the conditions that the temperature is 35-37 ℃ and the rotating speed is 150rpm, promoting the degradation of long-chain fatty acid, and improving the methane yield (92-124%) and the conversion rate of palmitic acid resources (17.02-42.81%), wherein calcium palmitate is particularly prominent. Compared with the prior art, the method has the advantages of improving the methane production rate of the waste oil, relieving the inhibition effect of long-chain fatty acid, and improving the activity of methanogens by anaerobic digestionAnd the method is easy to implement.

Description

Method for slowing down inhibition of palmitic acid on methane production of anaerobic digestion waste oil
Technical Field
The invention belongs to the field of anaerobic digestion of organic wastes, and particularly relates to a method for retarding inhibition of palmitic acid on methane production of anaerobic digestion waste oil
Background
In recent years, the treatment of organic waste and the recovery of biomass energy by anaerobic digestion have been receiving more and more attention. Under the trend, the waste oil and fat has higher methane production potential which is 2.4 times higher than carbohydrate and 2 times higher than protein, so the treatment mode of the waste oil and fat is also transferred from the traditional treatment mode and the anaerobic digestion technology for biodiesel.
Waste oils and fats also have problems in anaerobic digestion processes, wherein the degradation of long chain fatty acids is considered to be the rate limiting step in anaerobic digestion of waste oils and fats, since long chain fatty acids are easily adsorbed to the cell surface, hinder the transfer of nutrients and electrons, and also cause flotation of sludge to be flushed out of the reactor. It has been found that long chain fatty acids have a toxic and inhibitory effect on different anaerobically digested microorganisms (in concert with beta-oxidases and methanogens), which ultimately results in a reduced methanogenesis rate. The length of a fatty acid carbon chain in the waste oil is between C14 and C18, the content of palmitic acid (C16) is the highest, the palmitic acid is a main inhibiting factor, when the concentration of the palmitic acid reaches 5.71mmol/L, the methane yield of acetic acid type methanogens is reduced by 50%, and simultaneously, the acetic acid type methanogens have inhibiting effects on propionic acid degrading bacteria and a beta-oxidation process, so that the effect of slowing down the inhibition of the palmitic acid on the methane production of the anaerobic digestion waste oil is very important.
Disclosure of Invention
The invention aims to overcome the problems in the anaerobic digestion process of the waste oil and fat, and provides a method for reacting palmitic acid with different metal ions to generate a palmitic acid saponificate and seeking an optimal palmitic acid saponificate to slow down the inhibition effect of the palmitic acid on the anaerobic digestion of the waste oil and fat, improve the activity of microorganisms and improve the methane production rate and the methane yield.
In order to achieve the purpose, the invention is implemented according to the following technical scheme:
a method for improving methane production by anaerobic digestion of waste oil by using saponified substances of different metal ions comprises the following steps:
a method for slowing down the inhibition of palmitic acid on the methanogenesis of anaerobic digestion waste oil comprises the following steps:
(1) selecting palmitic acid saponificates
Step A: palmitic acid (C16) was used as a waste grease material.
And B: the metal ion adopts two valence states which are commonly used: (a) divalent: ca2+、Mg2+(ii) a (b) Trivalent: al (Al)3+、Fe3+。
And C: metal ions in the corresponding molar ratio (divalent ion: palmitic acid 1:2 and trivalent ion: palmitic acid 1:3) were reacted with palmitic acid.
Step D: and (3) generating a palmitic acid saponified substance through saponification: calcium palmitate, magnesium palmitate, aluminum palmitate and iron palmitate.
(2) Anaerobic digestion treatment mode
Step A: the ratio of palmitic acid saponified product to inoculum was 1(gVS/gVS), wherein the inoculum was selected from anaerobic digested sludge from Yanjing beer (Guilin Liquan) Inc. for treating beer wastewater.
And B: the reaction substrate and the inoculum are transferred to an anaerobic digestion bottle with a working volume of 150mL, and deionized water is added to the volume of 150 mL.
And C: in order to maintain anoxic conditions in the anaerobic digestion bottles, nitrogen is introduced into all the groups of sealed anaerobic digestion bottles for 5 minutes. Anaerobic digestion was then carried out at a temperature of 35-37 ℃ and a rotational speed of 150rpm, with periodic measurements of methane content.
Drawings
FIG. 1 cumulative methane production of metal saponificate
Detailed Description
The following embodiments of the present invention are provided, and it should be noted that the present invention is not limited to the following embodiments, and all equivalent changes based on the technical solutions of the present invention are within the protection scope of the present invention.
The method for improving anaerobic methanogenesis of waste oil and fat by using the palmitic acid saponificate of the embodiment of the invention is specifically described below.
Example 1
The embodiment provides a method for slowing down the inhibition of palmitic acid on methane production of anaerobic digestion waste oil, which specifically comprises the following steps:
in the embodiment, calcium palmitate is used as a reaction substrate, 3 groups of anaerobic digestion bottles with the total volume of 150mL are arranged, and 3 parallel samples are arranged in each group, namely the calcium palmitate group. In the initial stage of the reaction, the ratio of substrate to inoculum was 1: 1(gVS/gVS), respectively adding 20mL of inoculated sludge into each group of anaerobic digestion bottles, then adding corresponding amount of calcium palmitate, respectively introducing nitrogen into the anaerobic digestion bottles for 5min, discharging air, and keeping anaerobic conditions.
All anaerobic digestion bottles were subjected to anaerobic digestion at a temperature of 35-37 ℃ and a rotational speed of 150rpm, and the methane content was measured periodically. The cumulative methane production of the calcium palmitate group after 25 days of anaerobic digestion is shown in FIG. 1.
Example 2
The embodiment provides a method for slowing down the inhibition of palmitic acid on methane production of anaerobic digestion waste oil, which specifically comprises the following steps:
in the embodiment, magnesium palmitate is used as a reaction substrate, 3 groups of anaerobic digestion bottles with the total volume of 150mL are arranged, and 3 parallel samples are arranged in each group, namely the magnesium palmitate group. In the initial stage of the reaction, the ratio of substrate to inoculum was 1: 1(gVS/gVS) adding 20mL of inoculated sludge into each anaerobic digestion bottle, adding a corresponding amount of magnesium palmitate, introducing nitrogen into the anaerobic digestion bottles for 5min, discharging air, and keeping anaerobic conditions.
All anaerobic digestion bottles were subjected to anaerobic digestion at a temperature of 35-37 ℃ and a rotational speed of 150rpm, and the methane content was measured periodically. The cumulative methane production of the magnesium palmitate group after 25 days of anaerobic digestion is shown in FIG. 1.
Example 3
The embodiment provides a method for slowing down the inhibition of palmitic acid on methane production of anaerobic digestion waste oil, which specifically comprises the following steps:
in the embodiment, aluminum palmitate is used as a reaction substrate, 3 groups of anaerobic digestion bottles with the total volume of 150mL are arranged, and each group is provided with 3 parallel samples, namely an aluminum palmitate group. In the initial stage of the reaction, the ratio of substrate to inoculum was 1: 1(gVS/gVS), respectively adding 20mL of inoculated sludge into each group of anaerobic digestion bottles, then adding a corresponding amount of aluminum palmitate, respectively introducing 5min of nitrogen into the anaerobic digestion bottles, discharging air, and keeping anaerobic conditions.
All anaerobic digestion bottles were subjected to anaerobic digestion at a temperature of 35-37 ℃ and a rotational speed of 150rpm, and the methane content was measured periodically. The cumulative methane production of the aluminum palmitate group after 25 days of anaerobic digestion is shown in FIG. 1.
Example 4
The embodiment provides a method for slowing down the inhibition of palmitic acid on methane production of anaerobic digestion waste oil, which specifically comprises the following steps:
in the embodiment, ferric palmitate is used as a reaction substrate, 3 groups of anaerobic digestion bottles with the total volume of 150mL are arranged, and each group is provided with 3 parallel samples, namely the ferric palmitate group. In the initial stage of the reaction, the ratio of substrate to inoculum was 1: 1(gVS/gVS), respectively adding 20mL of inoculated sludge into each group of anaerobic digestion bottles, then adding corresponding amount of ferric palmitate, respectively introducing nitrogen into the anaerobic digestion bottles for 5min, discharging air, and keeping anaerobic conditions.
All anaerobic digestion bottles were subjected to anaerobic digestion at a temperature of 35-37 ℃ and a rotational speed of 150rpm, and the methane content was measured periodically. The cumulative methane production of the iron palmitate group after 25 days of anaerobic digestion is shown in figure 1.
Comparative examples
The embodiment provides a method for producing methane by anaerobic treatment of non-saponified palmitic acid, which specifically comprises the following steps:
in the embodiment, 1 group of anaerobic digestion bottles with the total volume of 150mL are arranged, and 3 parallel samples are arranged, namely the control group. In the initial stage of the reaction, the ratio of substrate to inoculum was 1: 1(gVS/gVS) adding 20mL of inoculated sludge into an anaerobic digestion bottle respectively, then adding a corresponding amount of non-saponified pretreated palmitic acid into the anaerobic digestion bottle, introducing nitrogen into the anaerobic digestion bottle for 5min respectively, discharging air, and keeping anaerobic conditions. All anaerobic digestion bottles were subjected to anaerobic digestion at a temperature of 35-37 ℃ and a rotational speed of 150rpm, and the methane content was measured periodically. The cumulative methane production of the control group after 25 days of anaerobic digestion reaction is shown in FIG. 1.
At the end of anaerobic digestion, the comparative example reached a cumulative methane production of 410.1mL/gVSaddedWhile the cumulative methane production of the calcium palmitate group of example 1 reached a maximum of 916.8mL/gVSaddedThe improvement is 124% compared with the comparative example; the cumulative methane yield in the magnesium palmitate group of example 2 was 868.5mL/gVSaddedImprovement over comparative example by 112 percent; cumulative methane production in the aluminum palmitate group of example 3 was 788.8mL/gVSaddedThe ratio is improved by 92 percent compared with the comparative example; cumulative methane production in the iron palmitate group of example 4 was 853.2mL/gVSaddedThe improvement is 108% over the comparative example.
In conclusion, different palmitic acid saponifiable matters have obvious promotion effect on the anaerobic digestion gas production performance of the waste oil, wherein the effect of the calcium palmitate on improving the anaerobic digestion methane production of the waste oil is better.

Claims (7)

1. A method for slowing down the inhibition of palmitic acid on the methane production of anaerobic digestion waste oil is characterized by comprising the following steps:
(1) saponification treatment: mixing palmitic acid and four metal ions (Ca)2+、Mg2+、Al3+、Fe3+) Generating a palmitic acid saponified substance through saponification; ,
(2) anaerobic digestion for methane production: adding the palmitic acid saponificate generated in the step (1) into an anaerobic digestion reactor, then adding an inoculum to ensure that the ratio of the palmitic acid saponificate to the inoculum is 1(gVS/gVS), and carrying out anaerobic digestion under the conditions of the temperature of 35-37 ℃ and the rotating speed of 150rpm to produce methane.
2. The method for slowing the inhibition of palmitic acid to the methanogenesis of anaerobically digested waste oils and fats according to claim 1, wherein said method comprises: palmitic acid (C16) is selected as a representative of the waste oil and fat in the step (1).
3. The method for slowing the inhibition of palmitic acid to the methanogenesis of anaerobically digested waste oils and fats according to claim 1, wherein said method comprises: the palmitic acid saponificate in the step (1) adopts metal ions (divalent: Ca) with different valence states2+、Mg2+And trivalent: al (Al)3 +、Fe3+) A saponified product formed with palmitic acid.
4. The method for slowing the inhibition of palmitic acid to the methanogenesis of anaerobically digested waste oils and fats according to claim 1, wherein said method comprises: the saponified material in the step (1) is formed by reacting metal ions and palmitic acid in a corresponding molar ratio (divalent ion: palmitic acid 1:2 and trivalent ion: palmitic acid 1: 3).
5. The anaerobic digestion reaction system according to claim 1, wherein: the method of adding the palmitic acid soap of claim 3 as a reaction substrate to an anaerobic digestion system, wherein the ratio of palmitic acid soap to inoculum is 1 (gVS/gVS).
6. The anaerobic digestion reaction system according to claim 5, wherein: the ratio of the working volume of the anaerobic digestion reactor to the volume of inoculum was 7.5: 1.
7. the anaerobic digestion reaction system according to claim 5, wherein: in order to keep the anaerobic condition in the anaerobic digestion bottles, introducing nitrogen into all the groups of sealed anaerobic digestion bottles for 5 minutes; anaerobic digestion was then carried out at 35-37 ℃ and 150rpm, with periodic measurements of methane content.
CN202110272607.5A 2021-03-12 2021-03-12 Method for slowing down inhibition of palmitic acid on methane production of anaerobic digestion waste oil Pending CN112960879A (en)

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113564208A (en) * 2021-06-30 2021-10-29 桂林理工大学 Method for improving methane production by anaerobic digestion of calcium oleate based on oleic acid solubilization
CN117265021A (en) * 2023-09-08 2023-12-22 标发生态(山东)有限公司 Treatment method for carrying out anaerobic digestion on high ammonia nitrogen waste through co-digestion

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Publication number Priority date Publication date Assignee Title
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CN103725715A (en) * 2013-12-30 2014-04-16 北京化工大学 Preparation method of calcium ion slow-release agent and method for improving anaerobic digestion of kitchen wastes
CN107382003A (en) * 2017-08-11 2017-11-24 东南大学 A kind of method for anaerobic sludge digestion based on pyrohydrolysis Yu chemical conditioning combined pretreatment
CN111356540A (en) * 2017-09-15 2020-06-30 伊米尔技术有限公司 Integrated water-based waste conversion system

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Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113564208A (en) * 2021-06-30 2021-10-29 桂林理工大学 Method for improving methane production by anaerobic digestion of calcium oleate based on oleic acid solubilization
CN117265021A (en) * 2023-09-08 2023-12-22 标发生态(山东)有限公司 Treatment method for carrying out anaerobic digestion on high ammonia nitrogen waste through co-digestion

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Application publication date: 20210615