CN115161352A - Method for improving production of methane by butyrate degradation through nano composite material - Google Patents

Method for improving production of methane by butyrate degradation through nano composite material Download PDF

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CN115161352A
CN115161352A CN202210744187.0A CN202210744187A CN115161352A CN 115161352 A CN115161352 A CN 115161352A CN 202210744187 A CN202210744187 A CN 202210744187A CN 115161352 A CN115161352 A CN 115161352A
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butyrate
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methane
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reduced graphene
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薛嵘
孙艳
张宝永
孙浩
郭艳
马云倩
王娜
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Qilu University of Technology
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Abstract

The invention belongs to the technical field of anaerobic digestion for methane production, and relates to a method for improving butyrate degradation for methane production by utilizing a nano composite material, wherein the nano composite material is used as an electron carrier, butyrate is used as an electron donor, and butyrate degradation is promoted under an anaerobic fermentation culture condition so as to improve methane yield; the nano composite material is a ferroferric oxide/reduced graphene oxide nano composite material, wherein the ferroferric oxide is 22-28% in mass content. In the invention, ferroferric oxide/reduced graphene oxide can serve as an electronic channel between microorganisms to promote interspecies direct electron transfer between methanogenic bacteria and methanogenic archaea. Direct inter-species electron transfer does not require H, as compared to conventional Intermediate Electron Transport (IET) 2 And formate-mediated electron transfer in anaerobic microorganismsFavorable conditions of thermodynamics and metabolism are created in the group, so that the butyrate is quickly converted into CH 4 。

Description

Method for improving production of methane by butyrate degradation through nano composite material
Technical Field
The invention belongs to the technical field of methane production through anaerobic digestion, and particularly relates to a method for improving the degradation of butyrate and methane production through a nano composite material.
Background
With the rapid development of society, the ever-increasing organic waste brings huge environmental and health threats to the society. At present, most of organic waste is treated by incineration, and harmful gases such as carbon monoxide, carbon dioxide, methane, formaldehyde, water vapor, nitrogen oxides, hydrogen sulfide and the like generated in the process can cause air pollution and global warming. In search for clean, economical ways to treat organic waste, attention has turned to anaerobic digestion processes. Anaerobic digestion is considered to be a promising method for renewable energy production. Anaerobic digestion is the most cost-effective of all biodegradable waste treatment technologies compared to other biological and thermochemical conversion processes. In recent years, anaerobic digestion has become more and more important in the treatment of industrial waste, municipal sewage, excess activated sludge and kitchen waste, mainly due to the advantages of low operating cost and high sludge load of anaerobic fermentation. Above all, it also produces CH of high calorific value 4 。
Anaerobic digestion is an effective solution for treating and utilizing organic waste to produce renewable resources. Anaerobic digestion mainly comprises four biochemical reaction stages: hydrolyzing, acidifying, producing hydrogen, producing acetic acid and producing methane. The main intermediate metabolite linking the hydrogen-producing acetogenic stage and the methanogenic stage is a short chain fatty acid, of which the more important one is butyrate. Butyrate serving as a key metabolite generated in the anaerobic degradation process of organic waste can be oxidized into acetate and H by hydrogen-producing acetic acid bacteria 2 /CO 2 Or a mixture of formates, which are then converted into CH by methanogens 4 。
The nano conductive material can promote the generation of methane through an interspecies direct electron transfer path among methanogenic microorganisms. The metal nanoparticles are fixed on the surface of a carbon-based carrier (such as graphene and multi-walled carbon nanotubes), so that the stability of the nanoparticles can be improved, the aggregation probability among the nanoparticles can be reduced, and the interaction between a nano material and microorganisms can be promoted.
Currently, known carbon-based composite materials applied to anaerobic fermentation mainly include iron sesquioxide/carbon nanoparticles, nickel-graphene nanocomposite, hydroxyapatite-graphene nanocomposite, iron sesquioxide biochar, zero-valent iron biochar, and magnetite biochar. However, these materials have no magnetism, specific surface area and conductivity much smaller than those of ferroferric oxide/reduced graphene oxide materials, and cannot provide more attachment sites for microorganisms and promote better interaction among microorganisms.
Disclosure of Invention
In order to overcome the problems of low methane yield and slow substrate degradation rate in anaerobic digestion in the prior art. The invention provides a method for improving the degradation of butyric acid and producing methane by a nano composite material. According to the method, the magnetite/reduced graphene oxide nanocomposite which is easy to contact with microorganisms is added in anaerobic digestion methane production to promote the interaction among the microorganisms, so that the degradation of butyrate is accelerated and the methane yield is improved. Meanwhile, the magnetite/reduced graphene oxide serves as a hydrogen storage device, hydrogen nutrition type methanogenesis in an anaerobic digestion system is promoted, and abundance of hydrogen nutrition methanogens (Methanobacterium) is improved.
In order to achieve the technical purpose, the technical scheme of the invention is as follows:
a method for improving the degradation of butyrate and the production of methane by utilizing a nano composite material is characterized in that the nano composite material is used as an electron carrier, butyrate is used as an electron donor, and the degradation of butyrate is promoted under the anaerobic fermentation culture condition so as to improve the yield of methane; the nano composite material is a ferroferric oxide/reduced graphene oxide nano composite material, wherein the ferroferric oxide is 22-28% in mass content.
Preferably, the method comprises the following steps: and mixing the inoculated sludge, sodium butyrate, a nitrogen source and the ferroferric oxide/reduced graphene oxide nanocomposite to form an anaerobic fermentation culture system, and carrying out anaerobic fermentation at the pH of 7.0 +/-0.1 and the temperature of 30-40 ℃ to produce methane.
Preferably, the anaerobic fermentation system uses a nitrogen stripping method to maintain an anaerobic environment inside the reactor.
Preferably, in the anaerobic fermentation culture system, the adding amount of the sodium butyrate is 7-9 g/L COD.
Preferably, in the anaerobic fermentation culture system, the nitrogen source is peptone, and the adding amount is 0.08-0.12 g/L.
Preferably, in the anaerobic fermentation culture system, the inoculation amount of the inoculated sludge accounts for 20-40% of the volume of the system.
Preferably, the physicochemical properties of the inoculated sludge are that the pH is 7.0 +/-0.1, and the total solid (TS, wt%): 9.89 ± 0.32, volatile solids (VS, wt% of TS): 73.94 +/-2.80, ammonia Nitrogen (NH) 4 + -N, mg/L): 693 ± 20.8, total organic carbon (TOC, mg/L): 440.6 ± 20, total carbon (TC, mg/L): 1204 ± 32.2, inorganic carbon (IC, mg/L): 763.1 ± 22.7, chemical oxygen demand (COD, mg/L): 1460.00 ± 32.00.
Further preferably, the inoculation sludge is obtained by acclimatization through the following steps:
(1) Collecting anaerobic sludge from an upflow anaerobic sludge blanket of a citric acid wastewater treatment plant;
(2) Adding 0.5-1 g/L glucose into the anaerobic sludge, and culturing for 20-30 days at the temperature of 30-40 ℃ to obtain the high-activity anaerobic microorganism inoculated sludge.
Preferably, in the anaerobic fermentation culture system, the addition amount of the ferroferric oxide/reduced graphene oxide nanocomposite is 50-400 mg/L. The content of ferroferric oxide in the ferroferric oxide/reduced graphene oxide nanocomposite is 22.79-27.57%, and the preferable content is 25-26%.
Preferably, the ferroferric oxide/reduced graphene oxide nanocomposite promotes the growth of methanogens Anaeroidea, syntropimonas, proteinihilum and Methanobacterium, constructs a potential interspecific direct electron transfer channel, promotes the methane production rate, and shortens the anaerobic methane production period.
The invention has the beneficial effects that:
in the invention, ferroferric oxide/reduced graphene oxide can serve as an electronic channel between microorganisms to promote interspecies direct electron transfer between methanogenic bacteria and methanogenic archaea. Direct inter-species electron transfer does not require H, as compared to conventional Intermediate Electron Transfer (IET) 2 And formate-mediated electron transfer, can create thermodynamically and metabolically favorable conditions in the anaerobic microbiome for rapid conversion of butyrate to CH 4 。
According to the invention, the ferroferric oxide/reduced graphene oxide formed by compounding the ferroferric oxide nanoparticles and the reduced graphene oxide (rGO) has the advantages of high surface area, excellent conductivity, good biocompatibility, capability of providing metal ions and the like. Ferroferric oxide/reduced graphene oxide can be adhered to the surfaces of microbial cells to form bridges between bacteria, so that the interspecies direct electron transfer between the bacteria is effectively promoted. Iron ions released by ferroferric oxide/reduced graphene oxide can not only react with Fe in dissimilatory iron reducing bacteria 3+ Reduction to Fe 2+ In the process, an anaerobic microenvironment with more reducibility is constructed, and the anaerobic microenvironment can also be used as an important trace element to stimulate the growth of methanogenic microorganisms. In addition, ferroferric oxide nanoparticles on the surface of the rGO are more easily utilized by methanogenic microorganisms, and the concentration of the ferroferric oxide nanoparticles in the ferroferric oxide/reduced graphene oxide nanocomposite is lower than that of single Nanoparticles (NPs) because the ferroferric oxide nanoparticles are well dispersed on the surface of the rGO and keep a certain distance, so that the toxicity is lower. In addition, ferroferric oxide/reduced graphene oxide can be easily recovered, and the release of the ferroferric oxide/reduced graphene oxide to the environment is reduced to the maximum extent.
Before the methane production experiment, the obtained sludge is cultured for 20-30 days to obtain high-activity anaerobic microorganism inoculated sludge, and then the experiment is carried out, and the methane production process is fast by adopting proper substrate addition amount. During anaerobic digestion, too high a substrate COD concentration can lead to inhibition, thereby prolonging the whole anaerobic digestion cycle.
Drawings
FIG. 1 shows biological CH by ferroferric oxide nanoparticles and different dosage of ferroferric oxide/reduced graphene oxide 4 Resulting in yield effects.
FIG. 2 shows biological CH by ferroferric oxide nanoparticles and different dosage of ferroferric oxide/reduced graphene oxide 4 The effect of yield.
FIG. 3 shows the influence of ferroferric oxide nanoparticles and different amounts of ferroferric oxide/reduced graphene oxide on the degradation of butyrate.
FIG. 4 shows the effect of ferroferric oxide nanoparticles added in an amount of 100mg/L on microbial communities.
FIG. 5 shows the effect of ferroferric oxide/reduced graphene oxide added in an amount of 100mg/L on microbial communities.
Detailed Description
The following examples are further illustrative of the present invention, but the present invention is not limited thereto. The raw materials used in the examples of the present invention were all common commercial products unless otherwise specified, wherein the magnetite nanoparticles were purchased from Macklin, shanghai, china, and had a purity of more than 99.5% and an average particle diameter of 100nm.
The size of the ferroferric oxide/reduced graphene oxide nano composite material used in the embodiment of the invention is 80-120 nm. The nano ferroferric oxide is uniformly distributed on the surface of the gauze-shaped reduced graphene oxide. X-ray diffraction data of the ferroferric oxide/reduced graphene oxide nanocomposite material: characteristic peaks at 18.44 degrees, 30.18 degrees, 35.56 degrees, 43.16 degrees, 53.6 degrees, 57.18 degrees and 62.78 degrees correspond to ferroferric oxide; the diffraction peak at 26.02 ° corresponds to the (002) plane of graphitic carbon. The preparation method of the ferroferric oxide/reduced graphene oxide nano-scale refers to Chinese patent document (202111579767.0).
Example 1
A method for improving the degradation of butyrate and the production of methane by utilizing a nano composite material,
ultrapure water, sodium butyrate, peptone, inoculated sludge and ferroferric oxide/reduced graphene oxide are added into a reactor with the effective volume of 500mL to form an anaerobic fermentation system. The anaerobic fermentation system comprises: 8g/L of COD sodium butyrate, 0.08-0.12 g/L of peptone, 150mL of inoculated sludge and 50mg/L of ferroferric oxide/reduced graphene oxide.
Adjusting the pH value in the reactor to 7.0 +/-0.1, blowing off the mixture by using nitrogen for 5-10 min to keep the interior of the reactor in an anaerobic environment, and performing butyric acid degradation and methane production reaction under a medium temperature condition (30-40 ℃) for 5 days. During the whole experiment, the fermentation broth was collected every 12h, filtered through a 0.45 μm filter membrane, and placed in a brown sample bottle to be tested. The methane production was measured every 12 h. The method for collecting methane is an alkali discharge method, and the method adopts 10 percent NaOH solution to absorb H in gas 2 S、CO 2 When the volume of the discharged NaOH solution is equal to that of the acidic gas, the volume of the discharged NaOH solution is CH 4 The yield of (2). In this example, the method for producing magnetite/reduced graphene oxide nanoparticles was the same as that described in example 1 of chinese patent document (114408981a, 202111579767.0). The results show that after the 5-day experiment, the degradation rate of the sodium butyrate is 100%, the accumulation amount of the methane is 158mL/gCOD, and the maximum degradation rate of the methane is 6.18 mL/(g COD.h).
Example 2
A method for improving the degradation of butyrate and producing methane by utilizing a nano composite material,
the same method as in example 1 was used, except that the amount of ferroferric oxide/reduced graphene oxide material added was 100mg/L. After the experiment was completed. The results show that the degradation rate of sodium butyrate is 100%, the cumulative yield of methane is 179.75mL/g COD, and the maximum methane production rate is 6.96 mL/(g COD.h). 16S rRNA high-throughput sequencing is used for evaluating the influence of ferroferric oxide/reduced graphene oxide on the microbial community in the process of producing methane by degrading anaerobic fermentation butyric acid.
Example 3
The method for improving the yield of methane by butyrate degradation by using the nano composite material is the same as that in example 1, and is characterized in that the dosage of ferroferric oxide/reduced graphene oxide is 200mg/L. The results show that the degradation rate of sodium butyrate is 100%, the cumulative yield of methane is 160.625mL/g COD, and the maximum methane production rate is 6.48 mL/(g COD.h).
Example 4
The method for improving the yield of methane by butyrate degradation by using the nano composite material is the same as that in the embodiment 1, and is characterized in that the adding amount of ferroferric oxide/reduced graphene oxide is 400mg/L. The results showed that the degradation rate of sodium butyrate was 100%, the cumulative yield of methane was 143.5mL/g COD, and the maximum methane production rate was 6.13 mL/(g COD. H).
Comparative example 1
A method for improving the production of methane through butyrate degradation by using a nanocomposite material, which is the same as that in example 1, is different in that a ferroferric oxide/reduced graphene oxide nanocomposite material is replaced by ferroferric oxide nanoparticles. Magnetite nanoparticles were provided by Macklin, shanghai, china, with a purity of greater than 99.5% and a diameter of 100nm. The dosage of the magnetite nano-particles is 50mg/L. The results show that the degradation rate of sodium butyrate is 100%, the cumulative yield of methane is 146.00mL/g COD, and the maximum methane production rate is 6.11 mL/(g COD.h).
Comparative example 2
A method for improving the methane production through butyrate degradation by utilizing a nano composite material is the same as that of comparative example 1, except that the adding amount of magnetite nanoparticles is 100mg/L. The results show that the degradation rate of sodium butyrate is 100%, the cumulative yield of methane is 162.38mL/g COD, and the maximum methane production rate is 6.56 mL/(g COD.h). After the experiment is finished, 16S rRNA high-throughput sequencing is used for evaluating the influence of the magnetite nanoparticles on the microbial community in the process of degrading the methanogenesis by anaerobic fermentation butyric acid.
Comparative example 3
A method for improving the methane production through butyrate degradation by utilizing a nano composite material is the same as that of comparative example 1, except that the adding amount of magnetite nano particles is 200mg/L. The results showed that the degradation rate of sodium butyrate was 100%, the cumulative yield of methane was 149.75mL/g COD, and the maximum methane production rate was 6.13 mL/(g COD. H).
Comparative example 4
A method for improving the methane production through butyrate degradation by utilizing a nano composite material is the same as that of comparative example 1, except that the adding amount of magnetite nano particles is 400mg/L. The results showed that the degradation rate of sodium butyrate was 100%, the cumulative yield of methane was 135.00mL/g COD, and the maximum methane production rate was 6.01 mL/(g COD. H).
Comparative example 5
A method for increasing the production of methane by butyrate degradation using a nanocomposite, using the same method as in comparative example 1, except that no material is added. The results showed that the degradation rate of sodium butyrate was 100%, the cumulative yield of methane was 128.88mL/gCOD, and the maximum methane production rate was 5.51 mL/(g COD. H).
From the data of examples 1 to 4 and comparative examples 1 to 5, it can be seen that the optimum addition amount of magnetite nanoparticles was 100mg/L, and the methane production and methane yield were increased by 25.99% and 19.09% in comparative example 2, respectively, as compared to comparative example 5. The optimal addition amount of the ferroferric oxide/reduced graphene oxide nanocomposite is 100mg/L, and compared with the comparative example 5, the yield and the yield of methane in example 2 are respectively improved by 39.48% and 26.28%. The methane production and yield were increased by 10.70% and 6.10%, respectively, compared to comparative example 2. In general, the magnetite/reduced graphene oxide nanocomposite has the optimal promotion effect on the degradation of butyrate to produce methane.
FIGS. 1-5 show magnetite nanoparticles and magnetite/reduced graphene oxide on biological CH in accordance with the present invention, respectively 4 The effect of the generation, the effect of magnetite nanoparticles and magnetite/reduced graphene oxide on butyrate degradation, the effect of magnetite nanoparticles and magnetite/reduced graphene oxide on microbial communities.
From FIGS. 1 and 2, it can be seen that the optimal methane yield, 162.38 and 179.75mL/g COD, is achieved when the concentration of magnetite nanoparticles and magnetite/reduced graphene oxide is increased from 50mg/L to 100mg/L. The ratio is 25.99% and 39.48% higher than that of the ratio 5, respectively, which shows that the magnetite nanoparticles and the magnetite/reduced graphene oxide with proper concentration have a promoting effect on the generation of methane through the degradation of butyrate in the anaerobic fermentation process, and the promoting effect of the magnetite/reduced graphene oxide is better than that of the magnetite nanoparticles. The peak in methane yield in both the examples and comparative examples occurred at 24h, indicating that both nano-additives did not have much effect on the hysteresis of the anaerobic fermenting microorganisms. When the concentration of magnetite nanoparticles and magnetite/reduced graphene oxide increased from 50mg/L to 100mg/L, the maximum methane yield was 6.56 and 6.96 mL/(gCOD · h), respectively, which was 19.09% and 26.28% higher than that of comparative example 5. This indicates that both magnetite nanoparticles and magnetite/reduced graphene oxide have a positive effect on the degradation of butyrate. The magnetite nanoparticles and the graphene both have high conductivity and can serve as electronic conduits among anaerobic microorganisms, and inter-species direct electron transfer in an anaerobic fermentation system is enhanced. Meanwhile, the magnetite nanoparticles and the magnetite/reduced graphene oxide can release iron ions, and the iron ions are important trace elements in anaerobic fermentation and can stimulate the growth of microorganisms and promote the generation of methane. Throughout the anaerobic fermentation process, it was observed that the magnetite/reduced graphene oxide yields methane were much higher than the magnetite nanoparticles.
As can be seen from FIG. 3, the butyrate in the reactor with the addition of 100mg/L magnetite nanoparticles and 100mg/L magnetite/reduced graphene oxide rapidly degraded to an extremely low content within 0-60 hours, whereas the butyrate in comparative example 5 achieved this effect within 96 hours. It was found that the decrease in butyrate concentration was almost simultaneous in example 2 and comparative example 2, but the CH produced in example 2 4 Significantly higher than in comparative example 2. This is because the addition of the magnetite/reduced graphene oxide material facilitates the hydrogenotrophic methanogenesis process of the anaerobic fermentation methanogenesis process.
FIG. 4 shows the microbial community structure of methanogens at the genus level in the reaction system after the anaerobic fermentation reaction. In the figure, R1 is comparative example 5, R2 is comparative example 2, R3 is example 2, and the most important species are Anaerolineaceae, bacteroides, syntropimonas and Proteiniphilum, and the abundance of the species accounts for 46.82-58.40% of the total flora. The Anaerolineaceae ratio is the highest, and the abundance of Anaerolineaceae in example 2 and comparative example 2 is higherImproved by 4.25% and 2.86% respectively compared with comparative example 5. Anaerolineceae is capable of oxidizing butyrate and transferring extracellular electrons to an electron acceptor. The high transcription of the pil A gene was detected in Anaerolineaceae, which is a prerequisite for the formation of conductive nanowires for interspecies direct electron transfer, and the presence of Anaerolineeae facilitates the conversion of substrates, the transfer of electrons between microbial species, and the promotion of methanogenesis. Syntropimonas is a butyrate oxidizing bacterium which can effectively promote the conversion of butyrate. The abundance of the bacterium in comparative example 2 and example 2 is respectively improved by 4.26% and 8.42% compared with comparative example 5, and the Syntropimonas is beneficial to the conversion of a substrate butyrate and can accelerate the reaction process. The relative abundance of Proteiphilium ranked 10.37% (example 2)>9.56% (comparative example 2)>5.17% (comparative example 5), proteinihilum are acid-producing bacteria that produce primarily acetic acid and propionic acid, but they also produce NH by protein degradation 3 . In conclusion, the abundance of Anaerolineaceae, syntropimonas and Proteiniphilum in anaerobic fermentation is increased in example 2 and comparative example 2, which shows that the magnetite nanoparticles and the magnetite/reduced graphene oxide are beneficial to the growth of the three bacteria, the degradation of butyrate is well promoted, the promotion effect of the magnetite/reduced graphene oxide is more remarkable than that of single magnetite or reduced graphene oxide, and the two have synergistic promotion effects on main methanogens.
FIG. 5 shows the change in microbial community structure of anaerobic methanogenic archaea at the genus level. In the figure, R1 is a comparative example 5, R2 is a comparative example 2, R3 is an example 2, methanobacterium and Methanosaeta are the most important microbial communities in anaerobic archaea, and account for 93.84% -95.73% of the total microbial communities. Methanobacterium is generally predominant in anaerobic fermentation and is capable of rapid synthesis of methane using a substrate. The higher the abundance, the more favorable the methane production. The abundance ratio of Methanobacterium in comparative example 2 and example 2 was increased by 3.05% and 7.43% respectively compared to comparative example 5.
Most methanobacteria are considered to be typical hydrogenotrophic methanogens that can convert carbon dioxide and hydrogen to methane. In the inter-species direct electron transfer mode,anaerolineceae, syntropimonas and Proteiniphilum are capable of oxidizing HAc to CO 2 Electrons are released outside cells, and the magnetite/reduced graphene oxide can be used as an electronic channel to transfer the electrons to Methanobacterium, so that CH can be rapidly and efficiently produced 4 . The result of the invention shows that the magnetite/reduced graphene oxide can help the electronically active bacteria Anaerolinaceae, syntrophomonas, proteinihilum and Methanobacterium to construct a potential interspecies direct electron transfer channel. The higher the abundance ratio of Anaerolineaceae, syntropimonas, proteinihilum and Methanobacterium in a dark fermentation system, the better the methane production effect.
While the foregoing is directed to the preferred embodiment of the present invention, it will be understood by those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention as defined in the appended claims.

Claims (10)

1. A method for improving the degradation of butyrate and the production of methane by utilizing a nano composite material is characterized in that the nano composite material is used as an electron carrier, butyrate is used as an electron donor, and under the anaerobic fermentation culture condition, the degradation of butyrate is promoted so as to improve the yield of methane; the nano composite material is a ferroferric oxide/reduced graphene oxide nano composite material, wherein the ferroferric oxide is 22-28% in mass content.
2. The method according to claim 1, characterized in that the method is: and mixing the inoculated sludge, sodium butyrate, a nitrogen source and the ferroferric oxide/reduced graphene oxide nanocomposite to form an anaerobic fermentation culture system, and carrying out anaerobic fermentation at the pH of 7.0 +/-0.1 and the temperature of 30-40 ℃ to produce methane.
3. The method of claim 2, wherein the anaerobic fermentation system maintains an anaerobic environment inside the reactor using a nitrogen stripping method.
4. The method according to claim 2, wherein the amount of sodium butyrate added in the anaerobic fermentation culture system is 7-9 g/L COD.
5. The method according to claim 2, wherein the nitrogen source is peptone and is added in an amount of 0.08 to 0.12g/L in the anaerobic fermentation culture system.
6. The method according to claim 2, wherein the inoculation amount of the sludge in the anaerobic fermentation culture system is 20-40% of the volume of the system.
7. A method according to claim 2, characterized in that the physicochemical properties of the inoculated sludge are pH 7.0 ± 0.1, total solids (TS, wt%): 9.89 ± 0.32, volatile solids (VS, wt% of TS): 73.94 +/-2.80, ammonia Nitrogen (NH) 4 + -N, mg/L): 693 ± 20.8, total organic carbon (TOC, mg/L): 440.6 ± 20, total carbon (TC, mg/L): 1204 ± 32.2, inorganic carbon (IC, mg/L): 763.1 ± 22.7, chemical oxygen demand (COD, mg/L): 1460.00 ± 32.00.
8. The method according to claim 7, wherein the inoculated sludge is acclimated by the following steps:
(1) Collecting anaerobic sludge from an upflow anaerobic sludge blanket of a citric acid wastewater treatment plant;
(2) Adding 0.5-1 g/L glucose into the anaerobic sludge, and culturing for 20-30 days at the temperature of 30-40 ℃ to obtain high-activity anaerobic microorganism inoculated sludge.
9. The method according to claim 2, wherein in the anaerobic fermentation culture system, the addition amount of the ferroferric oxide/reduced graphene oxide nanocomposite is 50-400 mg/L.
10. The method of claim 2, wherein the ferroferric oxide/reduced graphene oxide nanocomposite promotes the growth of the methanogens Anaeroidea, syntropimonas, proteinippilum and Methanobacterium.
CN202210744187.0A 2022-06-27 2022-06-27 Method for improving production of methane by butyrate degradation through nano composite material Pending CN115161352A (en)

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CN115992186A (en) * 2023-03-23 2023-04-21 山东生态家园环保股份有限公司 Method for improving hydrogen production performance of dark fermentation by utilizing nitrogen doped graphene

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115992186A (en) * 2023-03-23 2023-04-21 山东生态家园环保股份有限公司 Method for improving hydrogen production performance of dark fermentation by utilizing nitrogen doped graphene

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