CN117243226A - Preparation for controlling crop diseases and insect pests and preparation method thereof - Google Patents
Preparation for controlling crop diseases and insect pests and preparation method thereof Download PDFInfo
- Publication number
- CN117243226A CN117243226A CN202311208163.4A CN202311208163A CN117243226A CN 117243226 A CN117243226 A CN 117243226A CN 202311208163 A CN202311208163 A CN 202311208163A CN 117243226 A CN117243226 A CN 117243226A
- Authority
- CN
- China
- Prior art keywords
- liquid
- preparation
- microbial
- insect pests
- vinegar
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000002360 preparation method Methods 0.000 title claims abstract description 107
- 241000607479 Yersinia pestis Species 0.000 title claims abstract description 83
- 241000238631 Hexapoda Species 0.000 title claims abstract description 61
- 201000010099 disease Diseases 0.000 title claims abstract description 60
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 60
- 239000007788 liquid Substances 0.000 claims abstract description 143
- 230000000813 microbial effect Effects 0.000 claims abstract description 86
- 235000021419 vinegar Nutrition 0.000 claims abstract description 77
- 239000000052 vinegar Substances 0.000 claims abstract description 77
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 45
- 239000002131 composite material Substances 0.000 claims abstract description 34
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims abstract description 31
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 31
- 244000005700 microbiome Species 0.000 claims abstract description 27
- 239000000843 powder Substances 0.000 claims abstract description 27
- 150000001720 carbohydrates Chemical class 0.000 claims abstract description 24
- 241000186660 Lactobacillus Species 0.000 claims abstract description 23
- 229940039696 lactobacillus Drugs 0.000 claims abstract description 23
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 21
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 21
- 241000235342 Saccharomycetes Species 0.000 claims abstract description 17
- 241000186361 Actinobacteria <class> Species 0.000 claims abstract description 16
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000010413 sodium alginate Nutrition 0.000 claims abstract description 10
- 239000000661 sodium alginate Substances 0.000 claims abstract description 10
- 229940005550 sodium alginate Drugs 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 6
- 230000001580 bacterial effect Effects 0.000 claims description 57
- 238000003756 stirring Methods 0.000 claims description 38
- 238000002156 mixing Methods 0.000 claims description 36
- 241000894006 Bacteria Species 0.000 claims description 32
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 31
- 239000000203 mixture Substances 0.000 claims description 25
- 239000000243 solution Substances 0.000 claims description 24
- 235000014633 carbohydrates Nutrition 0.000 claims description 22
- 238000009472 formulation Methods 0.000 claims description 21
- 238000000855 fermentation Methods 0.000 claims description 20
- 230000004151 fermentation Effects 0.000 claims description 20
- 230000000243 photosynthetic effect Effects 0.000 claims description 17
- 239000011259 mixed solution Substances 0.000 claims description 16
- 238000001179 sorption measurement Methods 0.000 claims description 15
- 238000005273 aeration Methods 0.000 claims description 12
- 239000001963 growth medium Substances 0.000 claims description 12
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 10
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 10
- 241001446247 uncultured actinomycete Species 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- ZSBXGIUJOOQZMP-UHFFFAOYSA-N Isomatrine Natural products C1CCC2CN3C(=O)CCCC3C3C2N1CCC3 ZSBXGIUJOOQZMP-UHFFFAOYSA-N 0.000 claims description 8
- ZSBXGIUJOOQZMP-JLNYLFASSA-N Matrine Chemical compound C1CC[C@H]2CN3C(=O)CCC[C@@H]3[C@@H]3[C@H]2N1CCC3 ZSBXGIUJOOQZMP-JLNYLFASSA-N 0.000 claims description 8
- VQXSOUPNOZTNAI-UHFFFAOYSA-N Pyrethrin I Natural products CC(=CC1CC1C(=O)OC2CC(=O)C(=C2C)CC=C/C=C)C VQXSOUPNOZTNAI-UHFFFAOYSA-N 0.000 claims description 8
- 229930014456 matrine Natural products 0.000 claims description 8
- HYJYGLGUBUDSLJ-UHFFFAOYSA-N pyrethrin Natural products CCC(=O)OC1CC(=C)C2CC3OC3(C)C2C2OC(=O)C(=C)C12 HYJYGLGUBUDSLJ-UHFFFAOYSA-N 0.000 claims description 8
- VJFUPGQZSXIULQ-XIGJTORUSA-N pyrethrin II Chemical compound CC1(C)[C@H](/C=C(\C)C(=O)OC)[C@H]1C(=O)O[C@@H]1C(C)=C(C\C=C/C=C)C(=O)C1 VJFUPGQZSXIULQ-XIGJTORUSA-N 0.000 claims description 8
- 150000001875 compounds Chemical class 0.000 claims description 7
- 239000012153 distilled water Substances 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 239000002068 microbial inoculum Substances 0.000 claims description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 5
- 239000001888 Peptone Substances 0.000 claims description 5
- 108010080698 Peptones Proteins 0.000 claims description 5
- 239000008103 glucose Substances 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 235000013336 milk Nutrition 0.000 claims description 5
- 239000008267 milk Substances 0.000 claims description 5
- 210000004080 milk Anatomy 0.000 claims description 5
- 235000013557 nattō Nutrition 0.000 claims description 5
- 235000019319 peptone Nutrition 0.000 claims description 5
- 239000011780 sodium chloride Substances 0.000 claims description 5
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 claims description 4
- 239000004382 Amylase Substances 0.000 claims description 3
- 102000013142 Amylases Human genes 0.000 claims description 3
- 108010065511 Amylases Proteins 0.000 claims description 3
- YRMLFORXOOIJDR-UHFFFAOYSA-N Dichlormid Chemical compound ClC(Cl)C(=O)N(CC=C)CC=C YRMLFORXOOIJDR-UHFFFAOYSA-N 0.000 claims description 3
- 235000019418 amylase Nutrition 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims description 3
- 239000011790 ferrous sulphate Substances 0.000 claims description 3
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 3
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 3
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000004310 lactic acid Substances 0.000 claims description 2
- 235000014655 lactic acid Nutrition 0.000 claims description 2
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 42
- 239000000575 pesticide Substances 0.000 abstract description 21
- 230000012010 growth Effects 0.000 abstract description 18
- 239000000126 substance Substances 0.000 abstract description 16
- 239000003337 fertilizer Substances 0.000 abstract description 10
- 235000015097 nutrients Nutrition 0.000 abstract description 8
- 230000002195 synergetic effect Effects 0.000 abstract description 7
- 230000002265 prevention Effects 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 3
- 241000196324 Embryophyta Species 0.000 description 50
- 239000002689 soil Substances 0.000 description 19
- 230000009286 beneficial effect Effects 0.000 description 15
- 230000000052 comparative effect Effects 0.000 description 15
- 239000002023 wood Substances 0.000 description 12
- 235000013399 edible fruits Nutrition 0.000 description 11
- 239000003814 drug Substances 0.000 description 10
- 230000004083 survival effect Effects 0.000 description 10
- 238000012360 testing method Methods 0.000 description 10
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 8
- 235000017491 Bambusa tulda Nutrition 0.000 description 8
- 244000082204 Phyllostachys viridis Species 0.000 description 8
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 8
- 239000011425 bamboo Substances 0.000 description 8
- 230000002147 killing effect Effects 0.000 description 7
- 239000002994 raw material Substances 0.000 description 7
- 230000009467 reduction Effects 0.000 description 7
- 230000002829 reductive effect Effects 0.000 description 7
- 239000010902 straw Substances 0.000 description 7
- 235000002566 Capsicum Nutrition 0.000 description 6
- 239000003610 charcoal Substances 0.000 description 6
- 239000003085 diluting agent Substances 0.000 description 6
- 230000006872 improvement Effects 0.000 description 6
- 230000001965 increasing effect Effects 0.000 description 6
- 230000002401 inhibitory effect Effects 0.000 description 6
- DKIDEFUBRARXTE-UHFFFAOYSA-N 3-mercaptopropanoic acid Chemical compound OC(=O)CCS DKIDEFUBRARXTE-UHFFFAOYSA-N 0.000 description 5
- 241000426497 Chilo suppressalis Species 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 5
- 240000003889 Piper guineense Species 0.000 description 5
- 230000000853 biopesticidal effect Effects 0.000 description 5
- 239000006185 dispersion Substances 0.000 description 5
- 238000003973 irrigation Methods 0.000 description 5
- 230000002262 irrigation Effects 0.000 description 5
- 230000001737 promoting effect Effects 0.000 description 5
- NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonia chloride Chemical compound [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 4
- 241000233866 Fungi Species 0.000 description 4
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 4
- 230000008901 benefit Effects 0.000 description 4
- 238000011161 development Methods 0.000 description 4
- 230000018109 developmental process Effects 0.000 description 4
- 239000000284 extract Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 239000000047 product Substances 0.000 description 4
- 230000002035 prolonged effect Effects 0.000 description 4
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 description 3
- 244000025254 Cannabis sativa Species 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 3
- 240000007594 Oryza sativa Species 0.000 description 3
- 239000006002 Pepper Substances 0.000 description 3
- 235000016761 Piper aduncum Nutrition 0.000 description 3
- 235000017804 Piper guineense Nutrition 0.000 description 3
- 235000008184 Piper nigrum Nutrition 0.000 description 3
- 238000012271 agricultural production Methods 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 239000004615 ingredient Substances 0.000 description 3
- 230000002045 lasting effect Effects 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 238000005507 spraying Methods 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- 235000013311 vegetables Nutrition 0.000 description 3
- DSSYKIVIOFKYAU-XCBNKYQSSA-N (R)-camphor Chemical compound C1C[C@@]2(C)C(=O)C[C@@H]1C2(C)C DSSYKIVIOFKYAU-XCBNKYQSSA-N 0.000 description 2
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- 235000003261 Artemisia vulgaris Nutrition 0.000 description 2
- 240000006891 Artemisia vulgaris Species 0.000 description 2
- 244000061508 Eriobotrya japonica Species 0.000 description 2
- 235000009008 Eriobotrya japonica Nutrition 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 2
- 241000244206 Nematoda Species 0.000 description 2
- 241000233629 Phytophthora parasitica Species 0.000 description 2
- 241000758706 Piperaceae Species 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 238000005054 agglomeration Methods 0.000 description 2
- 230000002776 aggregation Effects 0.000 description 2
- 235000019270 ammonium chloride Nutrition 0.000 description 2
- 244000052616 bacterial pathogen Species 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 238000003763 carbonization Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 230000000749 insecticidal effect Effects 0.000 description 2
- 235000013379 molasses Nutrition 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 150000007524 organic acids Chemical class 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 230000001846 repelling effect Effects 0.000 description 2
- 238000002791 soaking Methods 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- OWEGMIWEEQEYGQ-UHFFFAOYSA-N 100676-05-9 Natural products OC1C(O)C(O)C(CO)OC1OCC1C(O)C(O)C(O)C(OC2C(OC(O)C(O)C2O)CO)O1 OWEGMIWEEQEYGQ-UHFFFAOYSA-N 0.000 description 1
- CCBICDLNWJRFPO-UHFFFAOYSA-N 2,6-dichloroindophenol Chemical compound C1=CC(O)=CC=C1N=C1C=C(Cl)C(=O)C(Cl)=C1 CCBICDLNWJRFPO-UHFFFAOYSA-N 0.000 description 1
- 241000238876 Acari Species 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241001124076 Aphididae Species 0.000 description 1
- 241000254127 Bemisia tabaci Species 0.000 description 1
- 235000012766 Cannabis sativa ssp. sativa var. sativa Nutrition 0.000 description 1
- 235000012765 Cannabis sativa ssp. sativa var. spontanea Nutrition 0.000 description 1
- 240000008574 Capsicum frutescens Species 0.000 description 1
- 229920002101 Chitin Polymers 0.000 description 1
- 239000005886 Chlorantraniliprole Substances 0.000 description 1
- 241000723346 Cinnamomum camphora Species 0.000 description 1
- 229920000742 Cotton Polymers 0.000 description 1
- 240000005636 Dryobalanops aromatica Species 0.000 description 1
- 229930091371 Fructose Natural products 0.000 description 1
- 239000005715 Fructose Substances 0.000 description 1
- RFSUNEUAIZKAJO-ARQDHWQXSA-N Fructose Chemical compound OC[C@H]1O[C@](O)(CO)[C@@H](O)[C@@H]1O RFSUNEUAIZKAJO-ARQDHWQXSA-N 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- 241000825107 Hierochloe Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 1
- GUBGYTABKSRVRQ-PICCSMPSSA-N Maltose Natural products O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@@H](CO)OC(O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-PICCSMPSSA-N 0.000 description 1
- 241000217407 Margaritifera Species 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 241000243785 Meloidogyne javanica Species 0.000 description 1
- 241000237536 Mytilus edulis Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 241000237509 Patinopecten sp. Species 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241000233614 Phytophthora Species 0.000 description 1
- 241000255969 Pieris brassicae Species 0.000 description 1
- 241000500437 Plutella xylostella Species 0.000 description 1
- 240000003768 Solanum lycopersicum Species 0.000 description 1
- 244000061456 Solanum tuberosum Species 0.000 description 1
- 235000002595 Solanum tuberosum Nutrition 0.000 description 1
- 241000985245 Spodoptera litura Species 0.000 description 1
- 241001454295 Tetranychidae Species 0.000 description 1
- 241001414989 Thysanoptera Species 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 235000009499 Vanilla fragrans Nutrition 0.000 description 1
- 244000263375 Vanilla tahitensis Species 0.000 description 1
- 235000012036 Vanilla tahitensis Nutrition 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000002154 agricultural waste Substances 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 229940027987 antiseptic and disinfectant phenol and derivative Drugs 0.000 description 1
- 239000012298 atmosphere Substances 0.000 description 1
- 244000000005 bacterial plant pathogen Species 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- GUBGYTABKSRVRQ-QUYVBRFLSA-N beta-maltose Chemical compound OC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O)[C@H](O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@@H]1O GUBGYTABKSRVRQ-QUYVBRFLSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 229910000019 calcium carbonate Inorganic materials 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 235000009120 camo Nutrition 0.000 description 1
- 229960000846 camphor Drugs 0.000 description 1
- 229930008380 camphor Natural products 0.000 description 1
- 239000001390 capsicum minimum Substances 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 235000005607 chanvre indien Nutrition 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- PSOVNZZNOMJUBI-UHFFFAOYSA-N chlorantraniliprole Chemical compound CNC(=O)C1=CC(Cl)=CC(C)=C1NC(=O)C1=CC(Br)=NN1C1=NC=CC=C1Cl PSOVNZZNOMJUBI-UHFFFAOYSA-N 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000004132 cross linking Methods 0.000 description 1
- 238000005520 cutting process Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000000428 dust Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 150000002148 esters Chemical class 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 244000037666 field crops Species 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000006870 function Effects 0.000 description 1
- 150000002240 furans Chemical class 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 125000002791 glucosyl group Chemical group C1([C@H](O)[C@@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000000227 grinding Methods 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 239000011487 hemp Substances 0.000 description 1
- 231100000086 high toxicity Toxicity 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 230000006698 induction Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 239000010410 layer Substances 0.000 description 1
- 230000000670 limiting effect Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 231100000053 low toxicity Toxicity 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000020638 mussel Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 231100000572 poisoning Toxicity 0.000 description 1
- 230000000607 poisoning effect Effects 0.000 description 1
- 235000012015 potatoes Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 230000000644 propagated effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- CQRYARSYNCAZFO-UHFFFAOYSA-N salicyl alcohol Chemical compound OCC1=CC=CC=C1O CQRYARSYNCAZFO-UHFFFAOYSA-N 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000020637 scallop Nutrition 0.000 description 1
- 238000007873 sieving Methods 0.000 description 1
- 244000000034 soilborne pathogen Species 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000004575 stone Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000002344 surface layer Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/10—Animals; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/12—Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/20—Fabaceae or Leguminosae [Pea or Legume family], e.g. pea, lentil, soybean, clover, acacia, honey locust, derris or millettia
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/24—Lauraceae [Laurel family], e.g. laurel, avocado, sassafras, cinnamon or camphor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N65/00—Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
- A01N65/08—Magnoliopsida [dicotyledons]
- A01N65/34—Rosaceae [Rose family], e.g. strawberry, hawthorn, plum, cherry, peach, apricot or almond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/02—Acaricides
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P7/00—Arthropodicides
- A01P7/04—Insecticides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/02—Enzymes or microbial cells immobilised on or in an organic carrier
- C12N11/10—Enzymes or microbial cells immobilised on or in an organic carrier the carrier being a carbohydrate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N11/00—Carrier-bound or immobilised enzymes; Carrier-bound or immobilised microbial cells; Preparation thereof
- C12N11/14—Enzymes or microbial cells immobilised on or in an inorganic carrier
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Plant Pathology (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Environmental Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Mycology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Pest Control & Pesticides (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Virology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Insects & Arthropods (AREA)
- Botany (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Molecular Biology (AREA)
- Inorganic Chemistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention provides a preparation for controlling crop diseases and insect pests and a preparation method thereof, belonging to the technical field of pesticide application, wherein the preparation comprises the following components: plant source vinegar, biochar, microbial agents and carbohydrate carbon sources; the microbial agent comprises a composite microbial liquid and a carrier, wherein microorganisms in the composite microbial liquid comprise bacillus natto, saccharomycetes, lactobacillus and actinomycetes, the carrier consists of sodium alginate, biochar and shell powder, and the weight ratio of the composite microbial liquid in the microbial agent is 5-50%. The components in the preparation are mixed to have a synergistic effect, the disease resistance, insect expelling and sterilization performance of the preparation can reduce the disease and pest incidence rate of crops, prevent and treat the disease and pest attack of two parts of overground plants and underground root systems, have a large prevention and treatment range, can quickly reduce the pest population density in a short period, have a quick knockdown speed, have obvious and durable disease and pest prevention and treatment effect, can provide nutrients for the crops, promote the growth of the crops, reduce the use amount of chemical fertilizers and pesticides, and improve the yield and quality of the crops.
Description
Technical Field
The invention relates to the technical field of pesticide application, in particular to a preparation for controlling crop diseases and insect pests and a preparation method thereof.
Background
Along with the improvement of the agricultural production level, the development of green food production has higher and higher requirements on fertilizer and pesticide for controlling diseases and insect pests used in the agricultural production. In the past agricultural cultivation activities, a large amount of chemical fertilizer is usually applied for increasing yield, and long-term use of the chemical fertilizer easily leads to shallow cultivation layers, excessively single nutrients, more diseases and insect pests of crops during the production and planting period, and serious influence on the quality and yield of agricultural products.
Most crops are easy to attack by diseases and insect pests in production and planting, and the diseases and insect pests have the characteristics of multiple types, wide distribution, quick transmission and serious damage loss, and can attack the diseases and insect pests on two parts of overground plants and underground root systems simultaneously. Therefore, chemical pesticides are mainly adopted to control crop diseases and insect pests in agricultural production, so that the application amount of the chemical pesticides is high. The chemical pesticides are used for a long time and repeatedly, so that the pollution to soil, water and atmosphere is generated, the pesticide residue in agricultural products is increased, the health and survival of human beings are directly endangered, the drug resistance and the drug resistance of various pests and harmful pathogens (including soil borne pathogens) to the chemical pesticides are improved year by year, the control difficulty is increased, the pest control cost of crops is also increased, and finally the yield of the crops is reduced. And the chemical pesticide kills natural enemies and other beneficial organisms and damages ecological balance while preventing and curing pests.
Therefore, the production and the use of high-toxicity and high-residue chemical pesticides are limited, and the safe, low-toxicity and environment-friendly biopesticide is greatly popularized and has become a promising development direction. With the research of technicians on biopesticides, whether single or combined biopesticides achieve a certain control effect on plant diseases and insect pests of crops, but the biopesticides have problems and difficulties restricting the use and development of the biopesticides, for example: the knockdown speed is slower, the effective period is shorter, the pest population density of pests cannot be reduced rapidly in a short period, and the like.
Disclosure of Invention
The invention provides a preparation for controlling crop diseases and insect pests and a preparation method thereof, wherein the components in the preparation are synergistic, the disease resistance, insect expelling and sterilization performance of the preparation can reduce the disease and insect pest incidence rate of crops, the control range is wide, the pest population density can be rapidly reduced in a short period, the knockdown speed is high, the disease and insect pest control effect is obvious and durable, the use amount of chemical fertilizers and pesticides can be reduced, and the yield and quality of crops can be improved.
In order to achieve the above purpose, the present invention provides the following technical solutions:
in a first aspect, the present invention provides a formulation for controlling crop diseases and pests, comprising: plant source vinegar, biochar, microbial agents and carbohydrate carbon sources; the microbial agent comprises a composite microbial liquid and a carrier, wherein microorganisms in the composite microbial liquid comprise bacillus natto, saccharomycetes, lactobacillus and actinomycetes, the carrier consists of sodium alginate, biochar and shell powder, and the weight ratio of the composite microbial liquid in the microbial agent is 5-50%.
In the technical scheme, the biochar has larger porosity and specific surface area, is generally alkaline, and can neutralize the acidity of plant source vinegar liquid, so that the self environment of the preparation is more suitable for the survival of microorganisms, and meanwhile, the biochar can be used as a carrier to provide nutrients for the growth of microorganisms, and the effectiveness and the lasting effect of microbial agents are prolonged. The bacteria in the microbial agent can generate resistance through competition, antibiotic, bacteriolysis and induction of plants, so that prevention and control effects on pests and pathogenic bacteria infection are generated.
The components in the preparation of the invention are mixed to play a synergistic effect, the disease resistance, insect expelling and sterilization performance of the preparation is beneficial to reducing the disease and pest incidence rate of crops, the disease and pest invasion of two parts of overground plants and underground root systems is prevented and treated, the prevention and treatment range is wide, the pest population density can be rapidly reduced in a short period, the knockdown speed is high, the disease and pest prevention and treatment effect is obvious and durable, nutrients can be provided for crops, the growth of the crops is promoted, the use amount of chemical fertilizers and pesticides is reduced, and therefore, the preparation has the advantages of disease and pest resistance, synergy, fertilizer and pesticide application amount reduction, crop yield and quality improvement, drug resistance is not easy to generate, and the energy efficiency of the preparation product is stable and durable.
According to the invention, the weight ratio of the plant source vinegar liquid to the charcoal to the microbial inoculum to the carbohydrate carbon source is 1 (1-3): 1-2.5): 0.01-0.1.
According to the invention, in the carrier, the weight ratio of sodium alginate to charcoal to shell powder is 1 (1-3) (0.5-1.5).
According to the present invention, the carbohydrate carbon source is at least one of glucose, lactose, maltose, fructose, hemp, molasses, and brown sugar. Preferably brown sugar. The carbohydrate carbon source is used for providing certain nutrients for microorganisms in the preparation and prolonging the effective survival time of the microorganisms.
According to the present invention, the plant-derived vinegar includes at least one of wood vinegar, bamboo vinegar, straw vinegar, and grass vinegar. The straw vinegar liquid takes agricultural wastes such as crop straws (such as cotton stalks, wheat stalks, corn stalks, rice stalks, soybean stalks and the like) and the like as raw materials, the wood vinegar liquid takes wood such as wood dust/branches and the like and residues of forestry processing as raw materials, the bamboo vinegar liquid takes southern bamboo as raw materials, the grass vinegar liquid takes herbaceous plants as raw materials, and the steam gas mixture led out after carbonization in carbonization equipment is condensed and separated to obtain reddish brown liquid which contains various organic compounds such as acid, alcohol, phenol, ester, furans and the like, and also contains various organic components and trace elements beneficial to crop growth, thereby effectively inhibiting the growth of plant pathogenic bacteria, preventing the occurrence of plant diseases and insect pests, and having good control effect and lasting effect in a certain time.
Preferably, the plant source vinegar is wood vinegar, bamboo vinegar and straw vinegar with the weight ratio of (1-1.5) 1 (1-3). Because the components of different raw materials have different properties, the components of the vinegar are more various by utilizing the vinegar from various sources, and the vinegar is more beneficial to providing comprehensive nutrients required by pest control and growth for plants.
According to the invention, the preparation process of the microbial agent comprises the following steps:
1) Preparing bacterial liquid: taking 100-120g of dry yeast, adding 250-350L of water and 1-3wt% of brown sugar, and fermenting at 35-45 ℃ for 5-9 days to obtain a saccharomycete liquid; crushing 100-150g natto, adding 250-350L water and 1-3wt% brown sugar, and performing aeration fermentation at 30-55deg.C for 2-4 days to obtain bacillus natto solution; taking 50-100g of lactobacillus and 100-300g of pure milk, adding 250-350L of water and 1-3wt% of brown sugar, and fermenting at 30-50 ℃ for 1-3 days to obtain lactobacillus liquid; inoculating actinomycetes with a inoculation concentration of 5-9% to a culture medium, and performing aeration fermentation at 30-60 ℃ for 3-5 days to obtain actinomycete bacterial liquid;
2) Preparing a composite microbial bacterial liquid: mixing bacillus natto liquid, saccharomycete liquid, lactobacillus liquid and actinomycete liquid according to proportion to obtain mixed bacillus liquid, adding 1-3wt% of brown sugar into the mixed bacillus liquid, and uniformly mixing to obtain compound microbial bacillus liquid;
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, stirring uniformly, and carrying out mixed adsorption to obtain the microbial agent.
When the microbial agent is prepared, a plurality of microorganisms are loaded on the carrier by utilizing the porous structure and the adsorption capacity of the carrier to prepare the microbial agent, so that the microorganisms are dormant or supported in the carrier, the bacterial content, the survival rate and the effective survival time of the microorganisms in the preparation are improved, the dispersion of the microbial agent in the preparation is facilitated, and the microorganisms can play a role stably and efficiently during pest control.
The biochar has larger porosity and specific surface area, can provide habitat for microorganisms, has stronger adsorption capacity, can improve the load capacity of the microorganisms, and can provide nutrients for the growth of the microorganisms. The shell powder is powder prepared by pulverizing and grinding shell, wherein the main components are calcium carbonate, chitin, and a small amount of amino acid and polysaccharide substances for providing certain nutrients for microorganism bacteria and prolonging the effective survival time of the microorganism. Sodium alginate is a natural polysaccharide with certain adhesiveness and is used for promoting cross-linking adsorption between other solid carriers and microorganisms and protecting the microorganisms from being in a moist environment.
According to the invention, in the composite microorganism bacterial liquid, bacillus natto bacterial liquid, saccharomycete bacterial liquid, lactobacillus bacterial liquid and actinomycete bacterial liquid are mixed according to the volume ratio of 2 (1-1.5) (0.5-1.0), and the number of viable bacteria in each bacterial liquid is not less than 1.0x10 9 cfu/mL. The bacillus natto belongs to bacillus, has a wide growth temperature range, is acid and alkali resistant, and can maintain high stability and high survival rate in an acidic environment. The bacillus natto, the saccharomycetes, the lactobacillus and the actinomycetes can form beneficial bacteria advantages on soil or plants, inhibit harmful bacteria from propagating, provide a microbial environment favorable for crop growth, and achieve the effects of inhibiting bacteria, preventing diseases and promoting growth.
According to the invention, the dissolved oxygen in the fermentation system is not less than 70% during aeration fermentation.
According to the invention, the culture medium for actinomycetes fermentation is: 1500g of yeast powder, 3000g of peptone, 3000g of glucose, 150g of dipotassium hydrogen phosphate, 150g of magnesium sulfate, 300g of sodium chloride, 500g of ferrous sulfate, 1500g of amylase, 90g of sodium hydroxide, 300g of dichlormid, 900g of shell powder, 300g of brown sugar and distilled water, and sterilizing for 30min at 120 ℃.
According to the invention, the composite microorganism bacterial liquid also comprises photosynthetic bacteria bacterial liquid; the volume ratio of photosynthetic bacteria bacterial liquid to bacillus natto bacterial liquid is (1-2): 1.
Preferably, the photosynthetic bacteria bacterial liquid is prepared as follows: inoculating photosynthetic bacteria to the culture medium at a inoculation concentration of 3-5%, and fermenting at 30-40deg.C for 3-5 days to obtain photosynthetic bacteria bacterial solution.
Preferably, the photosynthetic bacteria medium is: 900g of sodium acetate, 300g of ammonium chloride, 300g of potassium dihydrogen phosphate, 60g of magnesium sulfate, 300g of sodium chloride, 100g of sodium bicarbonate, 600g of yeast extract, 30g of peptone, 6g of glutamic acid, 300g of shell powder, 150g of brown sugar and distilled water are dissolved to 300L, and the mixture is sterilized at 120 ℃ for 30min.
The lactobacillus, actinomycetes and photosynthetic bacteria are activated and propagated to logarithmic phase before being used for preparing bacterial liquid, and the steps of activation and propagation are carried out according to the conventional method in the field, and are not described herein.
According to the invention, the shell powder in the carrier and the culture medium is from mussel shell, pearl shell, oyster shell, scallop shell, clam shell and/or clam shell. Along with the fermentation, the shell powder is converted into soluble and absorbable calcium salt in the culture medium to be absorbed and utilized by microorganisms, thereby being beneficial to promoting the growth and propagation of microorganisms, increasing the number of viable bacteria in bacterial liquid and further being beneficial to improving the number of beneficial bacteria of the preparation and the control effect thereof.
According to the invention, the formulation further comprises 0.1-1.0wt% of pyrethrin and 0.1-1.0wt% of matrine. Pyrethrin is a kind of broad-spectrum pesticide capable of preventing and curing several pests, matrine with contact killing effect on pests is a natural plant pesticide, and has the functions of contact killing and stomach poisoning.
According to the invention, the preparation also comprises 5-10wt% of traditional Chinese medicine vinegar. Preferably, the preparation process of the traditional Chinese medicine vinegar liquid comprises the following steps: mixing herba Medicaginis 5-10 weight parts, mugwort 3-8 weight parts, herb Hierochloes Adoratae 1-8 weight parts, eriobotrya japonica 2-5 weight parts, cinnamomum camphora 1-3 weight parts, crab shell 1-3 weight parts, and Margaritifera 2-10 weight parts, pulverizing to 80-100 mesh, adding wood vinegar at a ratio of 1g (3-5) mL, stirring at 45-60deg.C and 300-500rpm, soaking for 18-24 hr, standing, filtering, and collecting filtrate to obtain traditional Chinese medicinal vinegar extract. The traditional Chinese medicine vinegar liquid is prepared from a plurality of raw materials, and the active ingredients in the raw materials are extracted after being soaked in the wood vinegar liquid, so that the traditional Chinese medicine vinegar liquid is mutually tonifying, not only can be used for resisting bacteria and killing insects, but also has obvious control effects on plant diseases and insect pests occurring in the whole growth period of crops, and has the effects of promoting growth, improving fruit quality and the like after being applied to the crops.
According to the present invention, the formulation can be used for controlling plant diseases and insect pests including but not limited to seed damping off, bacterial wilt, rot, aphid, early/late blight, fusarium and gray mold, root knot nematode, root cutting worm, bemisia tabaci, spider mites, ticks, leaf moths, nematodes, rice stem borers, grubs, thrips, plutella xylostella, cabbage caterpillar, prodenia litura, saprophytic fungus, fusarium, phytophthora, leaf mold, flammule and the like.
The organic acid, phenol, derivatives thereof, microorganisms and the like contained in the preparation have the effects of killing or repelling common pests in crops, have the effect of killing or inhibiting common pathogens, can prevent the attack of the pests on overground plants and underground root systems, has obvious and durable pest control effect, and is also beneficial to the growth, the yield increase and the quality improvement of the crops.
In a second aspect, the present invention provides a method for preparing a formulation for controlling crop diseases and insect pests, the method comprising:
1) Mixing plant source vinegar solution and biochar in proportion, oscillating for 15-30min at 150-300r/min, standing for 2-3h, stirring to obtain premix;
2) Adding microbial agent into the premix, and magnetically stirring for 0.5-1h at the temperature of 30-35 ℃ and the speed of 100-200r/min to obtain a mixed solution;
3) Adding a sugar carbon source into the mixed solution, magnetically stirring for 15-30min at the temperature of 30-35 ℃ and the speed of 100-200r/min, and standing for 1-2h to obtain the preparation for controlling crop diseases and insect pests; the weight ratio of the plant source vinegar liquid, the biochar, the microbial agent and the carbohydrate carbon source is 1 (1-3)/(1-2.5)/(0.01-0.1).
In the preparation method, the plant source vinegar is adsorbed on the biochar, so that the acidity of the plant source vinegar can be neutralized, and the carbohydrate carbon source is adsorbed on the biochar and a carrier of a microbial agent for providing microbial nutrition, so that the effectiveness of the preparation is prolonged.
In a third aspect, the present invention provides a method for controlling crop pests, comprising: the preparation for controlling crop diseases and insect pests is taken, diluted to 50-300 times by adding water to form a diluted solution, and the diluted solution is applied at a single time with the dosage of 5-50L/mu and can be applied in the whole growth period of crops.
The preparation is preferably applied in a liquid state, is applied by diluting with water in a conventional way in the field, specifically, for example, 1g of the preparation is diluted with 50-300g of water and then applied, and the preparation can be used for preventing and controlling diseases and insect pests of crops in the whole growth period (such as seedling stage, field planting stage, fruit setting stage, full bearing stage and the like), can replace chemical pesticides, or is matched with the chemical pesticides to obviously reduce the application amount of the chemical pesticides.
According to the invention, the diluted solution of the preparation can be used for seed soaking, irrigation, foliar spraying, water-based irrigation, drip irrigation or root irrigation treatment of crops when the preparation is applied specifically. The preparation has effects of preventing and treating diseases and insect pests on leaf surfaces and root systems of crops, and can kill pests such as root nodule nematodes or prevent seed damping-off even if applied to soil, and increase the number of beneficial microorganisms (mainly bacteria and fungi) in the soil, thereby inhibiting the growth and reproduction of pathogenic bacteria in the soil.
In a fourth aspect, the present invention provides the use of a formulation for controlling crop disease and pest in improving the quality of crop fruits. The preparation of the invention can also be used as a biological fertilizer, can increase soil fertility, is used for improving the fruit quality of crops, realizes the yield and income increase of the crops, and improves the quality of the crops.
The preparation for controlling crop diseases and insect pests and the preparation method thereof have the following advantages:
1) The organic acid, phenol and derivatives thereof (from plant source vinegar liquid), microorganisms and the like contained in the preparation have the effects of killing or repelling common pests in crops, have the effect of killing or inhibiting common pathogens, can prevent the attack of the pests on two parts of overground plants and underground root systems, can quickly reduce the pest population density in a short period, has high knockdown speed, and can form beneficial bacteria in the growing environment of the crops, so that the effective period of the control effect of the preparation is prolonged, the control effect of the pests is obvious and durable, and the growth, the yield increase and the quality improvement of the crops are also facilitated.
2) The preparation is harmless to human and livestock, is environment-friendly, has the effects of improving soil fertility, improving the activity of roots and leaves of crops, enhancing stress resistance of the crops and improving the quality and yield of fruits of the crops, increases the yield of the crops by more than 10%, and can remarkably reduce the dosage of chemical pesticides and fertilizers, thereby being widely applied to the aspects of controlling and increasing the yield of crop diseases and insect pests, promoting ecological green agriculture and having wide application prospect and development space.
3) The preparation has various application modes, and the preparation adopts the biochar to neutralize the acidity of plant source vinegar liquid, so that the effectiveness and the lasting effect of microbial agents in the preparation are improved, and each fungus in the microbial agents is loaded on a porous carrier, so that the survival rate and the effective survival time of the fungus are prolonged, and each component can play a role in synergy, stability and efficiency in pest control, and has better control effect.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are also within the scope of the invention.
The following examples use instrumentation conventional in the art. The test materials, reagents, etc. used in the examples described below are commercially available in the specification conventional in the art unless otherwise specified. Those of skill or conditions not identified in the following examples may be performed according to the techniques or conditions described in the literature in this field or according to the product specifications.
In the present invention and the following examples, the concentration, the proportion, etc. not specifically described are weight concentration, the weight ratio, etc., and "%" all represent weight percentages, and "parts" all represent weight parts, which belong to the writing habit commonly used by those skilled in the art, so that the description thereof is omitted herein.
As an improvement of the foregoing, when the formulation for controlling crop diseases and insect pests contains more components, the preparation method of the formulation is as follows:
1) Mixing plant source vinegar solution, chinese medicinal vinegar solution, pyrethrin, matrine and biochar at a certain proportion, oscillating for 15-30min at 150-300r/min, standing for 2-3 hr, stirring to obtain premix;
2) Adding microbial agent into the premix, and magnetically stirring for 0.5-1h at the temperature of 30-35 ℃ and the speed of 100-200r/min to obtain a mixed solution;
3) Adding saccharide carbon source into the mixed solution, magnetically stirring at 30-35deg.C and 100-200r/min for 15-30min, and standing for 1-2 hr to obtain the final product.
As an improvement to the previous implementation, the mixed adsorption step in the preparation of the microbial agent is further optimized as follows: adding a carrier into the composite microbial liquid, adding a dispersed component accounting for 3-10% of the weight of the carrier, stirring uniformly, and carrying out mixed adsorption to obtain the microbial agent. The dispersing component contains mercaptopropionic acid and salicyl alcohol in the weight ratio of 1 (0.3-1.0). Excessive addition of the dispersing component has no obvious gain on the dispersing effect, and the cost is easy to increase; when the amount of the dispersion component is too small, the optimum dispersion effect cannot be achieved, and thus the amount of the dispersion component to be added is controlled to 3 to 10% by weight of the carrier.
In order to ensure that the biochar and the shell powder in the carrier can be uniformly mixed when the biochar and the shell powder are mixed with the composite microbial bacterial liquid, the agglomeration phenomenon is avoided, so that the dispersing component is selected to be added to combine the hydrophilic group of the dispersing component with the biochar, especially the shell powder, the dispersing component and the biochar exert synergistic effect to weaken the mutual attraction between the shell powder and the biochar molecules, improve the agglomeration phenomenon between the shell powder and the biochar, strengthen the dispersibility of the shell powder and the biochar in the bacterial liquid, help to improve the mixing adsorption efficiency of the carrier in the bacterial liquid, further improve the loading amount of the shell powder and the biochar on the microorganisms and the bacterial liquid in cooperation with sodium alginate, and improve the effectiveness and the duration of the preparation.
The dry yeast, lactic acid bacteria, actinomycetes and photosynthetic bacteria in the embodiment of the invention are purchased from the biological technology limited company of Jinan Jin Yuyuan, and the sources of the strains used in the preparation of the invention are not particularly limited and can be cultivated or purchased by themselves.
The present invention will be described in further detail with reference to examples. It is to be understood that the examples are for illustrative purposes only and are not intended to limit the scope of the present invention.
Example 1:
the preparation method of the preparation for controlling crop diseases and insect pests comprises the following steps:
1) Preparing bacterial liquid: taking 100g of dry yeast, adding 250L of water and 1wt% of brown sugar, and fermenting for 5 days at 35 ℃ to obtain a saccharomycete liquid; crushing 100g of natto, adding 250L of water and 1wt% of brown sugar, and performing aeration fermentation at 30 ℃ for 2 days to obtain a bacillus natto solution; 50g of lactobacillus and 100g of pure milk are taken, 250L of water and 1wt% of brown sugar are added, and fermentation is carried out for 1 day at 30 ℃ to obtain lactobacillus bacterial liquid.
Inoculating actinomycetes with a inoculation concentration of 5% onto a culture medium, and performing aeration fermentation at 30 ℃ for 3 days to obtain actinomycete bacterial liquid. The actinomycetes culture medium is as follows: 1500g of yeast powder, 3000g of peptone, 3000g of glucose, 150g of dipotassium hydrogen phosphate, 150g of magnesium sulfate, 300g of sodium chloride, 500g of ferrous sulfate, 1500g of amylase, 90g of sodium hydroxide, 300g of dichlormid, 900g of shell powder, 300g of brown sugar and distilled water, and sterilizing for 30min at 120 ℃.
In the aeration fermentation, the dissolved oxygen in the fermentation system is not less than 70%. The number of viable bacteria in each bacterial liquid is not less than 1.0X10 9 cfu/mL。
2) Preparing a composite microbial bacterial liquid: mixing bacillus natto liquid, saccharomycete liquid, lactobacillus liquid and actinomycete liquid according to the volume ratio of 2:1:0.5:0.5 to obtain mixed bacillus liquid, adding 1wt% of brown sugar into the mixed bacillus liquid, and uniformly mixing to obtain composite microbial bacillus liquid.
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, uniformly stirring, and carrying out mixed adsorption to obtain a microbial agent, wherein the weight ratio of the composite microbial liquid in the microbial agent is 5%; the weight ratio of the sodium alginate to the biochar to the shell powder in the carrier is 1:1:0.5.
4) Mixing plant source vinegar and biochar in proportion, oscillating for 15min at 150r/min, standing for 2h, and stirring to obtain premix.
5) And adding a microbial agent into the premix, and magnetically stirring for 0.5h at the temperature of 30 ℃ and the speed of 100r/min to obtain a mixed solution.
6) Adding a sugar carbon source into the mixed solution, magnetically stirring for 15min at 30 ℃ and 100r/min, and standing for 1h to obtain the preparation for controlling crop diseases and insect pests. In the preparation, the weight ratio of the plant source vinegar to the charcoal to the microbial inoculum to the carbohydrate carbon source is 1:1:1:0.01, the carbohydrate carbon source is glucose, and the plant source vinegar is wood vinegar.
Example 2:
the preparation method in this example differs from example 1 only in that:
1) Preparing bacterial liquid: taking 120g of dry yeast, adding 350L of water and 3wt% of brown sugar, and fermenting for 9 days at 45 ℃ to obtain a saccharomycete liquid; crushing 150g of natto, adding 350L of water and 3wt% of brown sugar, and performing aeration fermentation at 50 ℃ for 4 days to obtain a bacillus natto solution; taking 100g of lactobacillus and 300g of pure milk, adding 350L of water and 3wt% of brown sugar, and fermenting at 50 ℃ for 3 days to obtain lactobacillus bacterial liquid.
2) Preparing a composite microbial bacterial liquid: and (3) mixing the bacillus natto liquid, the saccharomycete liquid, the lactobacillus liquid and the actinomycete liquid according to the volume ratio of 2:1.5:1.5:1.0 to obtain a mixed bacterial liquid, adding 3wt% of brown sugar into the mixed bacterial liquid, and uniformly mixing to obtain the compound microbial bacterial liquid.
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, uniformly stirring, and carrying out mixed adsorption to obtain a microbial agent, wherein the weight ratio of the composite microbial liquid in the microbial agent is 50%; the weight ratio of the sodium alginate to the biochar to the shell powder in the carrier is 1:3:1.5.
4) Mixing plant source vinegar and biochar in proportion, oscillating for 30min at 300r/min, standing for 3h, and stirring to obtain premix.
5) And adding a microbial agent into the premix, and magnetically stirring for 1h at 35 ℃ and 200r/min to obtain a mixed solution.
6) Adding a sugar carbon source into the mixed solution, magnetically stirring for 30min at 35 ℃ and 200r/min, and standing for 2h to obtain the preparation for controlling crop diseases and insect pests. In the preparation, the weight ratio of the plant source vinegar to the charcoal to the microbial inoculum to the carbohydrate carbon source is 1:3:2.5:0.1, the carbohydrate carbon source is molasses, and the plant source vinegar is bamboo vinegar.
Example 3:
the preparation method in this example differs from example 1 only in that:
1) Preparing bacterial liquid: adding 300L of water and 2wt% of brown sugar into 120g of dry yeast, and fermenting for 7 days at 40 ℃ to obtain a saccharomycete liquid; crushing 120g of natto, adding 300L of water and 3wt% of brown sugar, and performing aeration fermentation at 45 ℃ for 3 days to obtain a bacillus natto solution; 70g of lactobacillus and 300g of pure milk are taken, 300L of water and 3wt% of brown sugar are added, and fermentation is carried out for 3 days at 40 ℃ to obtain lactobacillus bacterial liquid. Inoculating actinomycetes with a inoculation concentration of 7% onto a culture medium, and performing aeration fermentation at 40 ℃ for 4 days to obtain actinomycete bacterial liquid.
2) Preparing a composite microbial bacterial liquid: and (3) mixing the bacillus natto liquid, the saccharomycete liquid, the lactobacillus liquid and the actinomycete liquid according to the volume ratio of 2:1.3:1.0:0.8 to obtain a mixed bacterial liquid, adding 3wt% of brown sugar into the mixed bacterial liquid, and uniformly mixing to obtain the compound microbial bacterial liquid.
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, uniformly stirring, and carrying out mixed adsorption to obtain a microbial agent, wherein the weight ratio of the composite microbial liquid in the microbial agent is 30%; the weight ratio of the sodium alginate to the biochar to the shell powder in the carrier is 1:2.5:1.0.
4) Mixing plant source vinegar and biochar in proportion, oscillating for 20min at 200r/min, standing for 2h, and stirring to obtain premix.
5) And adding a microbial agent into the premix, and magnetically stirring for 1h at 35 ℃ and 150r/min to obtain a mixed solution.
6) Adding a sugar carbon source into the mixed solution, magnetically stirring for 15min at 30 ℃ and 200r/min, and standing for 1.5h to obtain the preparation for controlling crop diseases and insect pests. In the preparation, the weight ratio of the plant source vinegar to the charcoal to the microbial inoculum to the carbohydrate carbon source is 1:2:2:0.05, the carbohydrate carbon source is brown sugar, and the plant source vinegar is wood vinegar, bamboo vinegar and straw vinegar with the weight ratio of 1.5:1:2.
Example 4:
the preparation method in this example differs from that of example 3 only in that:
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, adding a dispersing component accounting for 5% of the weight of the carrier, uniformly stirring, and carrying out mixed adsorption to obtain the microbial agent, wherein the dispersing component contains mercaptopropionic acid and salicyclic alcohol, and the weight ratio of the mercaptopropionic acid to the salicyclic alcohol is 1:0.5.
Example 5:
the preparation method in this example differs from that of example 3 only in that:
2) Preparing a composite microbial bacterial liquid: and (3) mixing the photosynthetic bacteria liquid, the bacillus natto liquid, the saccharomycetes liquid, the lactobacillus liquid and the actinomycetes liquid according to the volume ratio of 3:2:1.3:1.0:0.8 to obtain a mixed bacterial liquid, adding 3wt% of brown sugar into the mixed bacterial liquid, and uniformly mixing to obtain the compound microbial bacterial liquid.
The photosynthetic bacteria bacterial liquid is prepared as follows: and inoculating photosynthetic bacteria to a culture medium at a inoculation concentration of 5%, and fermenting at 35 ℃ for 5 days to obtain photosynthetic bacteria bacterial solution. The photosynthetic bacteria culture medium is as follows: 900g of sodium acetate, 300g of ammonium chloride, 300g of potassium dihydrogen phosphate, 60g of magnesium sulfate, 300g of sodium chloride, 100g of sodium bicarbonate, 600g of yeast extract, 30g of peptone, 6g of glutamic acid, 300g of shell powder, 150g of brown sugar and distilled water are dissolved to 300L, and the mixture is sterilized at 120 ℃ for 30min.
Example 6:
the preparation method in this example differs from that of example 3 only in that:
4) Mixing plant source vinegar solution, chinese medicinal vinegar solution and biochar at a certain proportion, oscillating for 15min at 150r/min, standing for 2 hr, and stirring to obtain premixed solution. The addition amount of the traditional Chinese medicine vinegar extract is 8wt% of the weight of the preparation. The preparation process of the traditional Chinese medicine vinegar liquid comprises the following steps: 10 parts by weight of alfalfa, 5 parts by weight of mugwort, 6 parts by weight of vanilla, 5 parts by weight of loquat, 3 parts by weight of camphor, 3 parts by weight of crab shell and 8 parts by weight of drunkenness are taken and mixed, crushed to 100 meshes, wood vinegar is added according to the proportion of 1g to 5ml, stirred and soaked for 20 hours at the temperature of 45 ℃ and the speed of 400rpm, and the mixture is kept stand and filtered to obtain filtrate, thus obtaining the traditional Chinese medicine vinegar extract.
Example 7:
the preparation method in this example differs from that of example 3 only in that:
4) Mixing plant source vinegar, pyrethrin, matrine and biochar in proportion, oscillating for 20min at 200r/min, standing for 2h, and stirring to obtain premix. The addition amount of pyrethrin is 0.3wt% of the weight of the preparation, and the addition amount of matrine is 0.5wt% of the weight of the preparation.
Example 8:
the preparation method in this example differs from that of example 3 only in that:
2) Preparing a composite microbial bacterial liquid: and (3) mixing the photosynthetic bacteria liquid, the bacillus natto liquid, the saccharomycetes liquid, the lactobacillus liquid and the actinomycetes liquid according to the volume ratio of 4:2:1.3:1.0:0.8 to obtain a mixed bacterial liquid, adding 3wt% of brown sugar into the mixed bacterial liquid, and uniformly mixing to obtain the compound microbial bacterial liquid. The photosynthetic bacteria liquid was prepared by the procedure of example 5.
4) Mixing plant source vinegar solution, chinese medicinal vinegar solution, pyrethrin, matrine and biochar at a certain proportion, oscillating at 150r/min for 15min, standing for 2 hr, and stirring to obtain premixed solution. The addition amount of the traditional Chinese medicine vinegar liquid is 10wt% of the weight of the preparation, the addition amount of the pyrethrin is 0.5wt% of the weight of the preparation, and the addition amount of the matrine is 1.0wt% of the weight of the preparation. The above Chinese medicinal vinegar extract was prepared by the procedure of example 6.
Example 9:
the preparation method in this example differs from that of example 8 only in that:
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, adding a dispersing component accounting for 7% of the weight of the carrier, stirring uniformly, and carrying out mixed adsorption to obtain the microbial agent, wherein the dispersing component contains mercaptopropionic acid and salicyclic alcohol, and the weight ratio of the mercaptopropionic acid to the salicyclic alcohol is 1:0.8.
Comparative example 1:
the preparation method of the preparation for controlling crop diseases and insect pests is different from the preparation in the embodiment 3 in that no microbial agent is added, and the preparation method specifically comprises the following steps:
1) Mixing plant source vinegar and biochar in proportion, oscillating for 20min at 200r/min, standing for 2h, and stirring to obtain premix.
2) Adding a sugar carbon source into the premix, magnetically stirring for 15min at 30 ℃ and 200r/min, and standing for 1.5h to obtain the preparation for controlling crop diseases and insect pests. In the preparation, the weight ratio of the plant source vinegar to the charcoal to the carbohydrate carbon source is 1:2:0.05, the carbohydrate carbon source is brown sugar, and the plant source vinegar is wood vinegar, bamboo vinegar and straw vinegar with the weight ratio of 1.5:1:2.
Comparative example 2:
the preparation method of the preparation for controlling crop diseases and insect pests is different from example 3 in that no plant source vinegar is added into the preparation, and the preparation method specifically comprises the following steps:
step 1) -step 3) is consistent with step 1) -step 3) in example 3.
4) Mixing microbial agent and biochar in proportion, and magnetically stirring at 35 ℃ and 150r/min for 1h to obtain a mixed solution.
5) Adding a sugar carbon source into the mixed solution, magnetically stirring for 15min at 30 ℃ and 200r/min, and standing for 1.5h to obtain the preparation for controlling crop diseases and insect pests. In the preparation, the weight ratio of the biochar to the microbial agent to the carbohydrate carbon source is 2:2:0.05, and the carbohydrate carbon source is brown sugar.
Comparative example 3:
the preparation method of the preparation for controlling crop diseases and insect pests is different from example 3 in that no biochar is added into the preparation, and the preparation method specifically comprises the following steps:
step 1) -step 3) is consistent with step 1) -step 3) in example 3.
4) Adding microbial agent into plant source vinegar liquid, and magnetically stirring at 35 ℃ and 150r/min for 1h to obtain mixed solution.
5) Adding a sugar carbon source into the mixed solution, magnetically stirring for 15min at 30 ℃ and 200r/min, and standing for 1.5h to obtain the preparation for controlling crop diseases and insect pests. In the preparation, the weight ratio of the plant source vinegar to the microbial agent to the carbohydrate carbon source is 1:2:0.05, the carbohydrate carbon source is brown sugar, and the plant source vinegar is wood vinegar, bamboo vinegar and straw vinegar with the weight ratio of 1.5:1:2.
Comparative example 4:
the preparation method in this example differs from that of example 3 only in that:
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, adding the dispersed component-mercaptopropionic acid which is 5% of the weight of the carrier, stirring uniformly, and carrying out mixed adsorption to obtain the microbial agent.
Comparative example 5:
the preparation method in this example differs from that of example 3 only in that:
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, adding the dispersing component-salicylalcohol, wherein the weight of the dispersing component-salicylalcohol is 5% of that of the carrier, stirring uniformly, and carrying out mixed adsorption to obtain the microbial agent.
Test example 1:
test of insecticidal Effect in field
And (3) test design: the formulations prepared in examples 3-9 and comparative examples 1-5 were taken as test group samples, the unapplied as blank group and chlorantraniliprole as control group, each group was provided with 3 parallel cells, 42 cells in total, and the area of each cell was 30m 2 And (5) randomly arranging. When in application, each group of medicaments is diluted to 100 times by adding water to form a diluent, and the diluent is applied once at the dosage of 15L/mu.
The test method comprises the following steps: and 5 points of diagonal lines are adopted for sampling in each cell before the pesticide is applied by adopting fixed-point fixed-plant investigation, 10 rice plants with rice stem borers are selected in each point, and the number of the rice stem borers on the rice plants is counted by hanging a card and is used as the number of the insect population before the pesticide is applied in the cell. The leaf surface spraying and the front and back surface spraying are adopted. The survival number of rice stem borers on the fixed-point host crops is respectively investigated 1 and 5 days after the application, the number of pests and the rate of reduction of insect population are counted, the control effect of each treatment is calculated, the rate of reduction of insect population is corrected as the control effect, and the results are shown in Table 1. Wherein, the calculation formula is as follows:
the rate of reduction of insect population (%) = (number of live insects before administration-number of live insects after administration)/number of live insects before administration×100%, and the control effect (%) = (rate of reduction of insect population of treatment group-rate of reduction of insect population of control group)/(rate of reduction of insect population of 100-control region) ×100%.
Table 1 field control effect (unit:%)
The results show that the preparation of the embodiment of the invention has stronger insecticidal capability on rice stem borers, the effect is better than that of a control group, the control effect of the group of the embodiment can exceed 90% on the first day, the control effect of part of the group of the embodiment reaches 100% on the 5 th day, the effect is outstanding, the pest population density can be quickly reduced in a short period, and the knockdown speed is high. The results of comparative examples 3 to 4 and 4 to 5 revealed that the control effect of example 4 was significantly superior to that of example 3, and the effect of the dispersed components of comparative examples 4 to 5 was weaker when used alone than when used together in example 4, and the synergistic effect of the dispersed components was significant. The results of comparative example 3 and comparative examples 1-3 show that the ingredients in the preparation of the invention are compounded to have a synergistic effect, the control effect of example 3 is obviously better than that of comparative examples 1-3 when used alone, and the control of the compounded ingredients of example 3 is faster and more stable.
Test example 2:
control effect of different preparations on soil insects and soil diseases
The test method comprises the following steps: the formulations prepared in examples 3-4 and comparative examples 1-3 were sampled in test groups and non-administered as controls, each group being provided with 3 replicates. When in application, each group of medicaments is diluted to 50 times by adding water to form a diluent, and the diluent is applied once at the dosage of 20L/mu. Root irrigation is respectively carried out in the planting of field crop potatoes and vegetable peppers and tomatoes. Each crop or vegetable has 3 parallel cells, each cell having an area of 20m 2 And (3) arranging random granules. And (5) all plants in each cell are investigated, and the seedling protection rate and the control effect are calculated. Seedling retention (%) = (total number of plants-number of victims)/total number of plants×100. Control effect (%) = (treatment group rate of seedlings-control group rate of seedlings)/(1-control group rate of seedlings) ×100. The results are shown in Table 2.
TABLE 2 control effect of different formulations on soil-borne diseases and soil pests (unit:%)
The results show that the preparation of the embodiment of the invention has obvious control effect on soil-borne diseases and underground pests of field crops and vegetables, and the control effect of the embodiment 4 is obviously better than that of the embodiment 3, which shows that the dispersion component of the embodiment 4 promotes the increase of the immobilized amount of microorganisms, is beneficial to improving the number of beneficial bacteria in soil and further improving the microbial environment of the soil, thereby improving the effectiveness of the preparation; the results of comparative example 3 and comparative examples 1-3 show that the preparation of the invention stably and efficiently acts, the ingredients are compounded to have a synergistic effect, and the control effect of example 3 is obviously better than that of comparative examples 1-3 when used alone, thus being beneficial to improving the yield and quality of crops.
Test example 3:
influence of the preparation on the quality of Capsici fructus
The test method comprises the following steps: the preparation prepared in example 3-example 4 was taken as a test group sample, and the NPK organic ternary compound fertilizer 16-16-16 was taken as a control group, each group being provided with 3 parallels. When the preparation is applied, each group of preparation is diluted to 50 times by adding water to form a diluent, the diluent is applied once by watering with the dosage of 40L/mu, and the control group is applied once with the dosage of 10 kg/mu. Test variety of capsicum: sheep horn red number one. Taking earth surface layer (0-20 cm) soil in the same area of a planting area for planting peppers for years, removing broken stone impurities, sieving with a 5mm sieve, and evenly split charging into each culture basin, wherein 3kg of each basin is filled with 1L of distilled water, so that the soil reaches 100% of the field water holding capacity. Standing for one week, sowing the white seeds into soil, and pouring the same amount of water into each pot according to the soil moisture condition. When the cultivation is carried out to the fruiting period, measuring the plant height (from the soil surface to the highest point of the plant) by using a tape measure; measuring stem thickness (as close as possible to the soil surface) with vernier calipers; fresh weight and dry weight of fruits are weighed during the harvest period (the fruits are washed and put into an oven for 85 clean fixation for 30min and dried to constant weight at 65 ℃). The Vc content in the fresh pepper fruits is measured by adopting a 2, 6-dichloro indophenol method. The results are shown in Table 3.
TABLE 3 influence of formulations on Capsici fructus fruit quality
| Plant height cm | Thick stem mm | Fresh weight g/g | Dry weight g/min | Vc content mg/kg | |
| Example 3 | 28.36 | 7.7 | 8.67 | 0.81 | 337.81 |
| Example 4 | 29.71 | 8.1 | 9.81 | 0.96 | 346.16 |
| Control group | 26.32 | 7.1 | 6.36 | 0.62 | 319.54 |
The results show that the preparation disclosed by the invention can promote the growth of pepper plants, can obviously increase the number of pepper flowers in a flowering period, obviously increase the height and the thickness of stems of pepper plants in a fruiting period, and also improve the fruit quality of pepper, and is beneficial to improving the soil fertility, inhibiting plant diseases and insect pests, improving the fruit quality of crops and realizing the yield and income increase of crops after being applied in the period of crop planting.
It should be noted that, in the present invention, detailed steps of part of operations are not described in detail, but are known in the prior art by those skilled in the art, and thus are not described herein. Moreover, in the present invention, all features such as values, amounts, contents, and concentrations defined in numerical ranges or percentage ranges are for brevity and convenience only. Accordingly, the description of a numerical range or percentage range should be considered to cover and specifically disclose all possible sub-ranges and individual values (including integers and fractions) within the range.
Finally, it should be noted that the foregoing examples are only for illustrating the technical solution of the present invention, and not for limiting the same, and all possible combinations of the technical features in the various embodiments or examples are not described in the present invention, so long as there is no contradiction between the combinations of the technical features, and any combination of the technical features in the various embodiments or examples may be performed, and all possible combinations should be considered as the scope described in the present specification. Although the invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art will appreciate that; the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention.
Claims (10)
1. A formulation for controlling crop diseases and insect pests, comprising: plant source vinegar, biochar, microbial agents and carbohydrate carbon sources; the microbial agent comprises a composite microbial liquid and a carrier, wherein microorganisms in the composite microbial liquid comprise bacillus natto, saccharomycetes, lactobacillus and actinomycetes, the carrier comprises sodium alginate, biochar and shell powder, and the weight ratio of the composite microbial liquid in the microbial agent is 5-50%.
2. The formulation for controlling crop diseases and insect pests according to claim 1, wherein the weight ratio of the plant source vinegar, the biochar, the microbial inoculum and the carbohydrate carbon source is 1 (1-3): 1-2.5): 0.01-0.1.
3. The formulation for controlling crop diseases and insect pests according to claim 1, wherein the weight ratio of sodium alginate, biochar and shell powder in the carrier is 1 (1-3): 0.5-1.5.
4. The formulation for controlling crop diseases and insect pests according to claim 1, wherein the microbial agent is prepared by the following steps:
1) Preparing bacterial liquid: taking 100-120g of dry yeast, adding 250-350L of water and 1-3wt% of brown sugar, and fermenting at 35-45 ℃ for 5-9 days to obtain a saccharomycete liquid; crushing 100-150g natto, adding 250-350L water and 1-3wt% brown sugar, and performing aeration fermentation at 30-55deg.C for 2-4 days to obtain bacillus natto solution; taking 50-100g of lactobacillus and 100-300g of pure milk, adding 250-350L of water and 1-3wt% of brown sugar, and fermenting at 30-50 ℃ for 1-3 days to obtain lactobacillus liquid; inoculating actinomycetes with a inoculation concentration of 5-9% to a culture medium, and performing aeration fermentation at 30-60 ℃ for 3-5 days to obtain actinomycete bacterial liquid;
2) Preparing a composite microbial bacterial liquid: mixing bacillus natto liquid, saccharomycete liquid, lactobacillus liquid and actinomycete liquid according to proportion to obtain mixed bacillus liquid, adding 1-3wt% of brown sugar into the mixed bacillus liquid, and uniformly mixing to obtain compound microbial bacillus liquid;
3) Mixing and adsorbing: adding a carrier into the composite microbial liquid, stirring uniformly, and carrying out mixed adsorption to obtain the microbial agent.
5. The preparation for controlling crop diseases and insect pests according to claim 4, wherein the Bacillus natto, yeast, lactic acid bacteria and actinomycetes are mixed in a volume ratio of 2 (1-1.5): (0.5-1.5): (0.5-1.0) and the viable count in each bacterial liquid is not less than 1.0X10 9 cfu/mL。
6. The formulation for controlling crop diseases and insect pests according to claim 4, wherein the oxygen dissolved in the fermentation system is not less than 70% during the aeration fermentation.
7. The formulation for controlling crop diseases and insect pests according to claim 4, wherein the culture medium for fermentation of actinomycetes is: 1500g of yeast powder, 3000g of peptone, 3000g of glucose, 150g of dipotassium hydrogen phosphate, 150g of magnesium sulfate, 300g of sodium chloride, 500g of ferrous sulfate, 1500g of amylase, 90g of sodium hydroxide, 300g of dichlormid, 900g of shell powder, 300g of brown sugar and distilled water, and sterilizing for 30min at 120 ℃.
8. The formulation for controlling crop diseases and insect pests according to claim 5, wherein the composite microbial liquid further comprises photosynthetic bacteria liquid; the volume ratio of the photosynthetic bacteria bacterial liquid to the bacillus natto bacterial liquid is (1-2): 1.
9. The formulation for controlling crop diseases and insect pests according to claim 1, wherein the formulation further comprises 0.1-1.0wt% of pyrethrin and 0.1-1.0wt% of matrine.
10. A method for preparing a formulation for controlling crop diseases and insect pests, the method comprising:
1) Mixing plant source vinegar solution and biochar in proportion, oscillating for 15-30min at 150-300r/min, standing for 2-3h, stirring to obtain premix;
2) Adding microbial agent into the premix, and magnetically stirring for 0.5-1h at the temperature of 30-35 ℃ and the speed of 100-200r/min to obtain a mixed solution;
3) Adding a carbohydrate carbon source into the mixed solution, magnetically stirring for 15-30min at the temperature of 30-35 ℃ and the speed of 100-200r/min, and standing for 1-2h to obtain the crop disease and pest control preparation according to any one of claims 1-8;
the weight ratio of the plant source vinegar liquid to the biochar to the microbial inoculum to the carbohydrate carbon source is 1 (1-3)/(1-2.5)/(0.01-0.1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311208163.4A CN117243226A (en) | 2023-09-19 | 2023-09-19 | Preparation for controlling crop diseases and insect pests and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202311208163.4A CN117243226A (en) | 2023-09-19 | 2023-09-19 | Preparation for controlling crop diseases and insect pests and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN117243226A true CN117243226A (en) | 2023-12-19 |
Family
ID=89136252
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202311208163.4A Pending CN117243226A (en) | 2023-09-19 | 2023-09-19 | Preparation for controlling crop diseases and insect pests and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN117243226A (en) |
-
2023
- 2023-09-19 CN CN202311208163.4A patent/CN117243226A/en active Pending
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Zafar-ul-Hye et al. | Application of ACC-deaminase containing rhizobacteria with fertilizer improves maize production under drought and salinity stress | |
| CN101697736B (en) | Bacillus subtilis and propiconazole compound wettable sterilizing powder and use thereof | |
| EP2514815A1 (en) | Biocontrol strain for cucumber and watermelon continuous cropping wilt diseases and microbial organic fertilizer thereof | |
| CN109679884B (en) | Efficient corn growth-promoting bacterium capable of reducing application of nitrogen and phosphorus fertilizers and application thereof | |
| EP2255660B1 (en) | Biocontrol agent against soil-borne diseases | |
| CN114085793B (en) | Production method and application of paenibacillus picolinae microbial inoculum for preventing and treating plant fungal diseases | |
| CN105594698B (en) | A kind of preparation method of bacillus laterosporus wettable powder | |
| CN106305793A (en) | Compound bacterial manure used for prevention and control of cotton verticillium wilt and the preparation method thereof | |
| CN101270012A (en) | Novel microorganism medicine fertilizer and preparation method thereof | |
| CN112753516A (en) | Ecological cultivation method for intercropping bighead atractylodes rhizome and soybeans | |
| CN114605203A (en) | Compound microbial fertilizer and application thereof | |
| CN107417415A (en) | A kind of agricultural microbial agent and preparation method thereof | |
| CN112889843B (en) | Killed rice-germ bacillus wettable powder for preventing and treating citrus red spiders and application thereof | |
| CN110343628B (en) | Microbial agent containing candida ethanolate and application thereof | |
| CN111892440A (en) | Microbial fertilizer for preventing and treating potato scab and preparation method and use method thereof | |
| CN116918832A (en) | Composite biocontrol microbial agent, preparation method, application and application method | |
| US20200197736A1 (en) | Biological composition for degrading plant pesticide residues and the application thereof | |
| CN110540461A (en) | Composite microbial liquid fertilizer for preventing and treating strawberry anthracnose as well as preparation method and application thereof | |
| CN114258750B (en) | Method for improving strawberry continuous cropping obstacle soil | |
| Guji et al. | Compost enriched with effective microorganism and Bordeaux mixture on ginger bacterial wilt (Ralstonia solanaceurum) Epidemics in southwestern, Ethiopia | |
| CN117243226A (en) | Preparation for controlling crop diseases and insect pests and preparation method thereof | |
| CN107646899A (en) | A kind of green high-efficient rice seed dressing agent and its preparation method and application | |
| CN107227270A (en) | A kind of biocontrol bacteria in soil-borne diseases agent, a kind of soil-borne disease biological control matrix and preparation method thereof and application method | |
| CN113349223A (en) | Plant microorganism seed dressing agent and use method thereof | |
| CN112956385A (en) | Ecological cultivation method for intercropping Hubei fritillary and broad beans |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |