CN117242940A - Mimicry sterile culture device for tobacco seedlings - Google Patents

Abstract

The invention relates to the field of culture devices, in particular to a mimicry sterile culture device for tobacco seedlings, which comprises a machine body, wherein a door is arranged on one side of the machine body, a drawer is arranged below the door, a bracket is arranged in the drawer, a plurality of through holes I communicated with the outer wall are formed in the upper surface of the bracket, a centrifugal tube is arranged in each through hole I, an injector is arranged above each centrifugal tube, a filter screen is arranged at a suction head of the injector, sliding grooves I communicated with the outer wall are formed in the upper surface of the bracket, clamping assemblies are arranged in each sliding groove I, a fixing plate is connected to the inner wall of the machine body above the bracket, the injector can extract disinfection liquid in the centrifugal tube through the arranged material extracting assemblies, the tube cover of the centrifugal tube can be taken down and installed through the arranged clamping assemblies, different disinfection liquids can be injected into the centrifugal tube through the arranged displacement assemblies, and basic conditions are provided for comparison analysis culture of different bacterial seedling environments.

Description

Mimicry sterile culture device for tobacco seedlings

Technical Field

The invention relates to the technical field of culture devices, in particular to a mimicry sterile culture device for tobacco seedlings.

Background

In the tobacco cultivation experiment, the interaction mechanism (disease resistance, growth promotion and the like) of microorganisms and tobacco is researched, a large amount of microorganisms exist in the natural environment, the influence and the effect of single or multiple microorganisms on crops (tobacco) are researched, the environment and other microorganism variables are required to be excluded for research, and in the tobacco seedling cultivation process, single disinfection and sterilization or manual intervention can cause invasion of other strains while the influence of one bacterial colony is eliminated.

In the tobacco seedling culture process, an integrated device for sterilizing is absent, so that the secondary pollution caused by manpower is reduced, the mimicry experimental variable is unknown, and the better contrast experimental culture cannot be performed.

Disclosure of Invention

The invention aims to provide a mimicry sterile culture device for tobacco seedlings, which is used for solving the problems in the background technology.

In order to achieve the above purpose, the present invention provides the following technical solutions:

a mimicry sterile culture device for tobacco seedlings comprises a machine body, a door is arranged on one side of the machine body, a drawer is arranged below the door, a support is arranged in the drawer, a plurality of through holes I communicated with the outer wall are formed in the upper surface of the support, a centrifugal tube is arranged in each through hole I, an injector is arranged above each centrifugal tube, a filter screen is arranged at a suction head of the injector, a sliding groove I communicated with the outer wall is formed in each side of the support, a clamping assembly is arranged in each sliding groove I, the inner wall of the machine body is connected with a fixing plate above the support, an alcohol tank is arranged on one side upper surface of the fixing plate, a pumping assembly is arranged on one side of the alcohol tank, a lifting groove communicated with the inside is formed between the fixing plate and the drawer, a displacement assembly is arranged in the lifting groove, each clamping assembly is used for opening a cover of the centrifugal tube, and the pumping assembly is used for sucking liquid in the centrifugal tube through the injector, and the displacement assembly is used for injecting liquid into the centrifugal tube.

As a preferable scheme of the invention, each clamping assembly comprises a sliding rail, each sliding rail is positioned in a first sliding groove, a sliding block is arranged above each sliding rail, an electric telescopic rod is arranged on the upper surface of each sliding block, a moving block is arranged at the telescopic end of each electric telescopic rod, a moving groove communicated with the outer wall of one side is arranged in each moving block, a first servo motor is arranged in each telescopic moving groove, the output end of each first servo motor is connected with a first threaded rod, a sleeve is arranged at one end of each first threaded rod, which is far away from the first servo motor, and the other end of each sleeve penetrates out of the moving groove and extends to the outside to be connected with a clamping plate.

As a preferable scheme of the invention, each sliding block is in sliding connection with the sliding rail, the outer wall of each sliding block is in sliding connection with the inner wall of the sliding groove, each sleeve is in threaded connection with the outer wall of the threaded rod, and each clamping plate is in contact with the outer wall of the centrifugal tube.

As a preferable scheme of the invention, the material pumping assembly comprises a hydraulic telescopic rod, the hydraulic telescopic rod is fixedly connected with the upper surface of a fixed plate, the telescopic end of the hydraulic telescopic rod penetrates through the lower surface of the fixed plate and extends into a long groove of the fixed plate to be connected with a convex plate, the upper surface of the convex plate is provided with a second through hole communicated with one side outer wall at the first through hole, each injector is provided with a first circular groove communicated with one side outer wall and the second through hole at the edge of the injector, a second placing groove communicated with the second through hole is formed below each circular groove, a second spring is arranged in each placing groove, the other end of each second spring is connected with a second clamping block, two sides of the upper surface of the convex plate are provided with a groove, each groove is internally provided with an electric push rod, the telescopic ends of the two electric push rods are provided with a transverse plate, and the transverse plate is internally provided with a second circular groove communicated with the lower surface and the one side outer wall.

As a preferable scheme of the invention, the outer wall of each clamping block is in sliding connection with the inner wall of the second placing groove, and each clamping block is in contact with the outer wall of the injector.

As a preferable scheme of the invention, the displacement assembly comprises two hydraulic rods, each hydraulic rod is positioned in a lifting groove, the telescopic end of each hydraulic rod is connected with a connecting plate, a second sliding groove communicated with the outer wall of one side is formed in each connecting plate, a second servo motor is mounted on the inner wall of one side of each second sliding groove, the output end of each second servo motor is connected with a second threaded rod, a threaded plate is sleeved on the outer wall of each second threaded rod, and a second fixing groove is formed in each threaded plate.

As a preferable scheme of the invention, the bottom end of each hydraulic rod is fixed with the inner wall of the lifting groove by bolts, the outer wall of each connecting plate is in sliding connection with the inner wall of the lifting groove, one end of each second threaded rod is in rotary connection with the inner wall of the sliding groove, each threaded plate is in threaded connection with the second threaded rod, and the outer wall of each threaded plate is in sliding connection with the inner wall of the sliding groove.

As a preferable scheme of the invention, a sodium chlorate tank is arranged on one side of the alcohol tank, a sterile water tank is arranged on one side of the sodium chlorate tank, a flexible hose is hermetically linked at the bottom end of the alcohol tank, an alcohol M pipe is connected in a fixing groove II penetrating through the displacement assembly at the other end of the flexible hose, a sodium chlorate M pipe is connected in a fixing groove II penetrating through the displacement assembly at the other end of the flexible hose at the bottom end of the sodium chlorate tank, a sterile water M pipe is connected in a fixing groove II penetrating through the displacement assembly at the other end of the flexible hose at the bottom end of the sterile water tank, and the alcohol M pipe, the sodium chlorate M pipe and the M end of the sterile water M pipe penetrate through the lower surface of a convex plate in the displacement assembly and extend to the outside.

As a preferable scheme of the invention, a first placing groove communicated with a first through hole is formed below the sliding groove of the support, a first communicating fixing groove is formed on one side of each placing groove, an electric push rod is arranged in the first fixing groove, the telescopic end of the electric push rod is connected with a first clamping block, one end of the support is connected with two connecting rods, the outer walls of the two connecting rods are sleeved with a first spring, the other ends of the two connecting rods penetrate through the outer wall of the other side of the support and extend to the outside, a rotating motor is arranged between the connecting rods on one side of the support away from the first spring, and the output end of the rotating motor is connected with an arc rod.

As a preferable scheme of the invention, the first outer wall of the clamping block is in sliding connection with the first inner wall of the placing groove, the two ends of the connecting rod are fixedly connected with the inner wall of the drawer, the bracket is in sliding connection with the outer wall of the connecting rod, the bottom end of the rotating motor is fixedly connected with the inner wall of the drawer through bolts, and the arc-shaped rod is contacted with the outer wall of the bracket.

Compared with the prior art, the invention has the beneficial effects that:

1) The method realizes unified disinfection at the disinfection stage of the tobacco seedling culture front end, reduces the influence of various strains, and unifies experimental strain research variables.

2) The method reduces unstable factors caused in the artificial disinfection and cultivation process of the tobacco seedlings, and can evaluate single indexes of the tobacco seedling growth and cultivation environment.

Drawings

FIG. 1 is a schematic diagram of the overall structure of the present invention;

FIG. 2 is a cut-away view of the overall structure of the present invention;

FIG. 3 is a top cross-sectional view of a displacement assembly of the present invention;

FIG. 4 is a cut-away view of the body of the present invention;

FIG. 5 is a schematic view of a drawer of the present invention;

FIG. 6 is a schematic diagram of a pumping assembly and a displacement assembly according to the present invention;

FIG. 7 is an enlarged view of the invention at A;

fig. 8 is an enlarged view of the invention at B.

In the figure: 1. a body; 101. a door; 102. a drawer; 1021. a rotating electric machine; 1022. an arc-shaped rod; 103. a lifting groove; 2. a bracket; 201. a first through hole; 202. a first sliding groove; 203. placing a first groove; 204. a first fixing groove; 205. a connecting rod; 206. a first spring; 3. an electric push rod; 301. a clamping block I; 4. a material pumping assembly; 401. a hydraulic telescopic rod; 402. a convex plate; 403. a circular groove I; 404. a second through hole; 405. a second placing groove; 406. a second spring; 407. a clamping block II; 408. a groove; 409. an electric push rod; 410. a cross plate; 411. a circular groove II; 5. a clamping assembly; 501. a slide rail; 502. a slide block; 503. an electric telescopic rod; 504. a moving block; 505. a moving groove; 506. a servo motor I; 507. a first threaded rod; 508. a sleeve; 509. a clamping plate; 6. a displacement assembly; 601. a hydraulic rod; 602. a connecting plate; 603. a second sliding groove; 604. a servo motor II; 605. a second threaded rod; 606. a thread plate; 607. a second fixing groove; 7. a fixing plate; 701. an alcohol tank; 7011. an alcohol M pipe; 702. a sodium chlorate tank; 7021. sodium chlorate M tube; 703. an aseptic water tank; 7031. sterile water M tube; 704. a flexible hose; 8. centrifuging tube; 9. a syringe.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention. The embodiments described below by referring to the drawings are illustrative only and are not to be construed as limiting the invention.

Hereinafter, an embodiment of the present invention will be described in accordance with its entire structure.

Examples

Referring to fig. 1-8, a mimicry aseptic culture device for tobacco seedlings comprises a machine body 1, a door 101 is arranged at one side of the machine body 1, a drawer 102 is arranged below the door 101, a support 2 is arranged in the drawer 102, a plurality of through holes I201 communicated with the outer wall are formed in the upper surface of the support 2, a centrifugal tube 8 is arranged in the through holes I201, an injector 9 is arranged above the centrifugal tube 8, a filter screen is arranged at a suction head of the injector 9, sliding grooves I202 communicated with the outer wall are formed in the upper surface of the support 2 at two sides of the through holes I201, a placing groove I203 communicated with the through holes I201 is formed in the lower portion of the sliding grooves I202, a fixing groove I204 communicated with one side of the placing groove I203, an electric push rod 3 is arranged in the fixing groove I204, a clamping block I301 is connected to a telescopic end of the electric push rod 3, two connecting rods 205 are connected to one end of the support 2, the outer wall of the connecting rod 205 is sleeved with a first spring 206, the other end of the connecting rod 205 penetrates through the outer wall of the other side of the bracket 2 and extends to the outside, a rotating motor 1021 is arranged between the connecting rods 205 on one side of the bracket 2 far away from the first spring 206, the output end of the rotating motor 1021 is connected with an arc rod 1022, the outer wall of a clamping block 301 is in sliding connection with the inner wall of a placing groove 203, the two ends of the connecting rod 205 are fixedly connected with the inner wall of the drawer 102, the bracket 2 is in sliding connection with the outer wall of the connecting rod 205, the bottom end of the rotating motor 1021 is fixedly bolted with the inner wall of the drawer 102, the arc rod 1022 is contacted with the outer wall of the bracket 2, a clamping assembly 5 is arranged in the sliding groove 202, the inner wall of the machine body 1 is connected with a fixing plate 7 above the bracket 2, an alcohol tank 701 is arranged on the upper surface of one side of the fixing plate 7, a sodium chlorate tank 702 is arranged on one side of the alcohol tank 701, a sterile water tank 703 is arranged on one side of the sodium chlorate tank 702, the bottom end of the alcohol tank 701 is hermetically connected with a flexible hose 704, the other end of the flexible hose 704 penetrates through a second fixing groove 607 in the carry moving assembly 6 to be connected with an alcohol M pipe 7011, the bottom end of the sodium chlorate tank 702 is hermetically connected with the flexible hose 704, the other end of the flexible hose 704 penetrates through the second fixing groove 607 in the carry moving assembly 6 to be connected with a sodium chlorate M pipe 7021, the bottom end of the sterile water tank 703 is hermetically connected with the flexible hose 704, the other end of the flexible hose 704 penetrates through the second fixing groove 607 in the carry moving assembly 6 to be connected with a sterile water M pipe 7031, the alcohol M pipe 7011, the sodium chlorate M pipe 7021 and the M end of the sterile water M pipe 7031 penetrate through the lower surface of the convex plate 402 in the moving assembly 6 and extend to the outside, one side of the upper surface of the fixing plate 7, which is located on the alcohol tank 701, a lifting groove 103 communicated with the inside is formed between the fixing plate 7 and the drawer 102, a moving assembly 6 is formed in the inner wall of the machine body 1, a clamping assembly 5 is used for opening a cover of the centrifuge tube 8, the liquid in the centrifuge tube 8 is sucked by the syringe 9, and the liquid in the centrifuge tube 8 is injected into the centrifuge tube 6.

When tobacco seedlings are required to be subjected to aseptic mimicry culture, a worker puts 50mm dry compressed Jiffy-7 matrix blocks into a plastic box, adds 1L of newly prepared 0.5 XLS nutrient solution into the box, fully dips the matrix blocks into the nutrient solution, waits for 30-40mm of the matrix blocks to expand to about 5 times of height, puts the matrix blocks after water absorption expansion into an empty plastic flowerpot, transfers two empty plastic flowerpots into the center of each culture box, transfers four fully hydrated matrix blocks into each culture box on two sides of the central plastic flowerpot, adds 50ml of redundant LS nutrient solution into each culture box, loosely puts the culture box cover on the box, puts two assembled culture boxes with loose covers into an autoclave, puts the assembled device into the autoclave after the packaging bags are sealed and folded, after autoclaving at 121 ℃ for 45min, cooling to about 50 ℃, then placing the assembled device at RT, when the seeds need to be disinfected and sterilized the next day after the first sterilization, a worker withdraws the drawer 102, places the centrifuge tube 8 containing a proper amount of tobacco seeds on the bracket 2, pushes the drawer 102 into the machine body 1, then opens the door 101, places the syringe 9 into the through hole II 404 of the convex plate 402, places the piston rod cap of the syringe 9 into the circular groove II 411 of the transverse plate 410, closes the door 101, at this time, the worker starts the clamping assembly 5 through the external controller to drive the tube cover to move upwards until the tube cover moves out of the tube opening of the centrifuge tube 8, then starts the electric push rod 3 to work, the telescopic end of the electric push rod 3 pushes the clamping block I301 to clamp the centrifuge tube 8, simultaneously starts the displacement assembly 6 to fill alcohol into the centrifuge tube 8, when the clamping assembly 5 drives the tube cap of the centrifuge tube 8 to repeatedly cover the tube cap on the centrifuge tube 8 just after the tube cap is taken out, the rotating motor 1021 works after the tube cap is covered, the output end of the rotating motor 1021 drives the arc rod 1022 to rotate, the rotating arc rod 1022 pushes the support 2 to move to one side when contacting with the outer wall of the support 2, the moving support 2 transversely slides along the connecting rod 205 and contacts with the first spring 206, the support 2 is swayed back and forth through the first spring 206, after the tobacco seeds fully contact with alcohol, the rotating motor 1021 stops working, then the tube cap opening operation of the centrifuge tube 8 is repeated, the material sucking assembly 4 is started to suck the alcohol, then the injector 9 is reset, at this time, the injector 9 filled with liquid is replaced by staff, then the operation of sterilizing the alcohol is repeated to inject sodium chlorate and sterile water to perform multiple sterilization operations on the tobacco seeds, taking out the sterilized tobacco seeds, respectively streaking and inoculating the solution of the last washed seeds on R2A and PDA culture medium by a disposable inoculating loop, culturing at 30 ℃ for at least one week, detecting whether the seeds are polluted by bacteria and fungi, standing for 2 days (36-48 h) by a sterilizing device, repeating the high-pressure sterilizing cycle, cooling for 3-4h or enough at RT, taking out sterilizing package, closing a cover in a sterilizing bag, wiping a laboratory sterile operation table by 75% alcohol, sterilizing the inner environment of the sterile operation table and all objects required to be used by alcohol spraying and ultraviolet lamp surface, taking out a culture box containing gnotolot from the sterilizing bag after sterilizing for at least 15 minutes, taking out the bag from the sterile operation table, opening the culture box, spooning a small amount of peat substrate or solution in a culture box from any part of the gnotoptops by a disposable inoculating loop, respectively coating the peat substrate or solution on R2A and PDA culture mediums, culturing at 30 ℃ for at least one week, detecting whether the substrate is polluted by bacteria and fungi, adding 15mL of 1 XLS nutrition solution into each gnotops by irrigating the top of each substrate block, and recovering the water content of the substrate block to a saturated state.

Can make syringe 9 take out the disinfection liquid in centrifuging tube 8 through the material subassembly 4 that takes out that sets up, can take off and install centrifuging tube 8's tube cap through the clamping component 5 that sets up, the displacement subassembly 6 that the rethread set up can make to pour into disinfection liquid into in centrifuging tube 8, ensures that tobacco seeds can be in place in one step when disinfecting to reduce personnel operating time improvement efficiency.

Referring to fig. 2, 3, 5 and 8, the clamping assembly 5 includes a sliding rail 501, the sliding rail 501 is located in a first sliding groove 202, a sliding block 502 is disposed above the sliding rail 501, an electric telescopic rod 503 is mounted on an upper surface of the sliding block 502, a moving block 504 is mounted at a telescopic end of the electric telescopic rod 503, a moving groove 505 communicated with an outer wall of one side is formed in the moving block 504, a first servo motor 506 is mounted in each telescopic moving groove 505, an output end of the first servo motor 506 is connected with a first threaded rod 507, a sleeve 508 is disposed at one end of the first threaded rod 507 far away from the first servo motor 506, the other end of the sleeve 508 penetrates out of the moving groove 505 and extends to the outside and is connected with a clamping plate 509, the sliding block 502 is slidably connected with the sliding rail 501, the outer wall of the sliding block 502 is slidably connected with an inner wall of the first sliding groove 202, the sleeve 508 is in threaded connection with an outer wall of the first threaded rod 507, and the clamping plate 509 is in contact with an outer wall of the centrifuge tube 8.

The staff starts servo motor one 506 through external control ware work, and the output of servo motor one 506 of work is through driving threaded rod one 507 rotatory, and rotatory threaded rod one 507 drives grip block 509 through sleeve 508 and carries out the centre gripping to centrifuging tube 8's tube cap, and after the centre gripping is accomplished, electric telescopic rod 503 upwards moves and takes the tube cap to shift up, until the tube cap shifts out centrifuging tube 8's mouth of pipe, then slider 502 passes through electric telescopic rod 503 and drives the tube cap and move to one side along slide rail 501.

Referring to fig. 2, 6 and 7, the material drawing component 4 includes a hydraulic telescopic rod 401, the hydraulic telescopic rod 401 is fixedly connected with the upper surface of the fixed plate 7, a telescopic end of the hydraulic telescopic rod 401 penetrates through the lower surface of the fixed plate 7 and extends to a long groove of the fixed plate 7 to be connected with a convex plate 402, a through hole two 404 communicated with one side outer wall is formed in the position of the through hole two 201 on the upper surface of the convex plate 402, the injector 9 is located in the through hole two 404, circular grooves 403 communicated with the one side outer wall and the through hole two 404 are formed in the position of the convex plate 402 along the plate, a placing groove two 405 communicated with the through hole two 404 is formed below the circular grooves two 403, a spring two 406 is installed in the placing groove two 405, the other end of the spring two 406 is connected with a clamping block two 407, a groove 408 is formed in two sides of the upper surface of the convex plate 402, an electric push rod 409 is installed in the groove 408, a transverse plate 410 is installed at the telescopic end of the electric push rod 409, a circular groove 411 communicated with the lower surface and one side outer wall is formed in the transverse plate 410, two outer walls 407 are respectively contacted with the two outer walls of the placing block two 407 and the two clamping blocks 405 are respectively connected with the two outer walls of the injector 9 in a sliding mode.

The hydraulic telescopic rod 401 is started to work, the telescopic end of the hydraulic telescopic rod 401 drives the convex plate 402 to move downwards, the downwards moved convex plate 402 drives the injector 9 to move downwards until the injector 9 is completely consistent with the centrifuge tube 8, the electric push rod 409 is started to work, the telescopic end of the electric push rod 409 pushes the transverse plate 410 to move upwards, and the moving transverse plate 410 drives the injection rod of the injector 9 to move upwards.

Referring to fig. 2, 3, 4 and 6, the displacement assembly 6 includes two hydraulic rods 601, the hydraulic rods 601 are all located in the lifting groove 103, the telescopic ends of the hydraulic rods 601 are all connected with a connecting plate 602, a second sliding groove 603 communicated with one side outer wall is formed in the connecting plate 602, a second servo motor 604 is mounted on one side inner wall of the second sliding groove 603, the output end of the second servo motor 604 is connected with a second threaded rod 605, a threaded plate 606 is sleeved on the outer wall of the second threaded rod 605, a second fixing groove 607 is formed in the threaded plate 606, the bottom ends of the hydraulic rods 601 are all fixed with the inner wall of the lifting groove 103 through bolts, the outer wall of the connecting plate 602 is connected with the inner wall of the lifting groove 103 in a sliding mode, one end of the second threaded rod 605 is connected with the inner wall of the second sliding groove 603 in a rotating mode, the threaded plate 606 is connected with the second threaded rod 605 through threads, and the outer wall of the threaded plate 606 is connected with the inner wall of the second sliding groove 603 in a sliding mode.

Starting the hydraulic rod 601 to push the connecting plate 602 to move downwards, the downwards moving connecting plate 602 drives the threaded plate 606 to move downwards, when the connecting plate moves to a certain position, the second servo motor 604 starts to work, the output end of the second servo motor 604 drives the second threaded rod 605 to rotate, the second threaded rod 605 drives the threaded plate 606 in threaded connection to move towards the centrifuge tube 8 until the pipe orifices of the alcohol M pipe 7011, the sodium chlorate M pipe 7021 and the sterile water M pipe 7031 are parallel to the pipe orifice of the centrifuge tube 8, and the hydraulic rod 601 continues to move downwards until the three pipe orifices extend into the centrifuge tube 8.

The titration rotation is controlled between the components and the equipment through PLC software.

The working flow of the invention is as follows: when the tobacco seedling is required to be subjected to aseptic mimicry culture experiments, a worker puts 50mm dry compressed Jiffy-7 matrix blocks into a plastic box, adds 1L of newly prepared 0.5 XLS nutrient solution into the box, fully dips the matrix blocks into the nutrient solution, swells to about 5 times of the height after 30-40mm of the matrix blocks, puts the matrix blocks after water absorption swelling into an empty plastic flowerpot, transfers two empty plastic flowerpots into the center of each culture box, transfers four fully hydrated matrix blocks into each culture box on two sides of the central plastic flowerpot, adds 50ml of redundant LS nutrient solution into each culture box, loosely puts a culture box cover on the box, puts two assembled culture boxes with loose covers into an autoclave, puts the assembled device into an autoclave after the packaging bag is sealed and folded, after autoclaving at 121 ℃ for 45min, cooling to about 50 ℃, then placing the assembled device at RT, when the seeds need to be disinfected and sterilized the next day after the first sterilization, a worker pulls out the drawer 102, places the centrifuge tube 8 containing a proper amount of tobacco seeds on the bracket 2, pushes the drawer 102 into the machine body 1, then opens the door 101, places the injector 9 into the through hole II 404 of the convex plate 402, places the piston rod cap of the injector 9 into the circular groove II 411 of the transverse plate 410, closes the door 101, at this time, the worker starts the servo motor I506 to work through the external controller, the output end of the working servo motor I506 rotates through the driving threaded rod I507, the rotating threaded rod I507 drives the clamping plate 509 through the sleeve 508 to clamp the tube cover of the centrifuge tube 8, after the clamping is completed, the electric telescopic rod 503 moves the tube cover upwards, until the tube cap moves out of the tube orifice of the centrifuge tube 8, the sliding block 502 drives the tube cap to move to one side along the sliding rail 501 through the electric telescopic rod 503, then the electric push rod 3 is started to work, the telescopic end of the electric push rod 3 pushes the clamping block I301 to clamp the centrifuge tube 8, meanwhile, the hydraulic rod 601 is started to push the connecting plate 602 to move downwards, the connecting plate 602 which moves downwards drives the threaded plate 606 to move downwards, when the connecting plate moves to a certain position, the servo motor II 604 starts to work, the output end of the servo motor II 604 which works drives the threaded rod II 605 to rotate, the rotating threaded rod II 605 drives the threaded plate 606 which is in threaded connection to move towards the centrifuge tube 8 until the tube orifices of the alcohol M tube 7011, the sodium chlorate M tube 7021 and the sterile water M tube 7031 are parallel to the tube orifice of the centrifuge tube 8, the hydraulic rod 601 continues to move downwards until the three tube orifices extend into the centrifuge tube 8, then the electromagnetic valve on the alcohol tank 701 starts the alcohol to be filled into the centrifuge tube 8 along with the alcohol M tube 7011, when the clamping assembly 5 drives the tube cover of the centrifuge tube 8 to repeatedly cover the tube cover on the centrifuge tube 8 just after the tube cover is taken out, the rotating motor 1021 works after the tube cover is covered, the output end of the rotating motor 1021 drives the arc rod 1022 to rotate, the rotating arc rod 1022 pushes the support 2 to move to one side when contacting with the outer wall of the support 2, the moving support 2 transversely slides along the connecting rod 205 and contacts with the first spring 206, the support 2 is swayed back and forth through the first spring 206, after the tobacco seeds fully contact with the alcohol, the rotating motor 1021 stops working, then the opening operation of the tube cover 8 is repeated, the hydraulic telescopic rod 401 is started to work, the telescopic end of the hydraulic telescopic rod 401 drives the convex plate 402 to move downwards, the downward convex plate 402 drives the injector 9 to move downwards until the injector 9 is completely consistent with the centrifuge tube 8, starting the electric push rod 409 to work, pushing the transverse plate 410 to move upwards by the telescopic end of the electric push rod 409, driving the injection rod of the injector 9 to move upwards by the movable transverse plate 410, sucking out the sterilizing liquid in the centrifuge tube 8 by vacuum drawing, resetting the injector 9, replacing the injector 9 filled with the liquid by a worker at the moment, repeating the operation of alcohol sterilization to inject sodium chlorate and sterile water to sterilize tobacco seeds for a plurality of times until sterilization is finished, respectively streaking and inoculating a solution of the last washed seed to R2A and PDA culture media by using a disposable inoculating loop until sterilization is finished, culturing at least one week at 30 ℃, detecting whether the seed is polluted by bacteria and fungi, placing a sterilizing device for 2 days (36-48 h), taking out a sterilizing package after cooling for 3-4h or enough cooling under RT, closing a cover in a sterilizing bag, using a 75% alcohol laboratory sterile operating table, spraying alcohol and sterilizing all articles required to the surfaces of the sterile operating table, taking out a solution of the sterilizing bag from the sterilizing bag after at least 15 minutes, respectively streaking and inoculating the solution to R2A and PDA, and taking out a small amount of the solution from the sterilizing bag to the bag after the sterilizing bag, and filling the solution into the bag after the sterilizing bag, and taking out a small amount of the solution from the bag to the bag after the sterilizing bag to a small amount of the bag to be saturated and a small amount of the substrate, and at least one week, and taking the substrate from the bag to be taken out of the sterile substrate and a moisture and a small amount of the substrate and a saturated substrate and a 1 and a saturated substrate and a moisture amount and a bag and at least one and a small amount of a substrate and a bag and is taken.

And then taking out the sterilized tobacco seeds uniformly, and transferring the tobacco seeds into a biochemical incubator for one time in a sterile experimental environment to realize connection. The drawer 2 is also provided with a glass cover plate which is covered before transfer to a subsequent operation.

Although embodiments of the present invention have been shown and described, it will be understood by those skilled in the art that various changes, modifications, substitutions and alterations can be made therein without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims (10)

1. A mimicry sterile culture device for tobacco seedlings, comprising a machine body (1), characterized in that: a door (101) is arranged on one side of the machine body (1), a drawer (102) is arranged below the door (101) and positioned in the drawer (102), a support (2) is arranged in the drawer (102), a plurality of through holes I (201) communicated with the outer wall are formed in the upper surface of the support (2), a centrifuge tube (8) is arranged in each through hole I (201), a syringe (9) is arranged above each centrifuge tube (8), a filter screen is arranged at a suction head of the syringe (9), sliding grooves I (202) communicated with the outer wall are formed in the upper surface of the support (2) and positioned on two sides of the through hole I (201), clamping components (5) are arranged in each sliding groove I (202), an alcohol tank (701) is arranged on the upper surface of one side of the support (2), a material drawing component (4) is arranged on one side of the alcohol tank (701), a lifting component (103) is arranged between the inner wall (7) of the support (1) and the drawer (102), a lifting component (103) is arranged in the lifting component (6) and is arranged in the lifting component (103), the material sucking component (4) is used for sucking the liquid in the centrifuge tube (8) by the injector (9), and the displacement component (6) is used for injecting the liquid into the centrifuge tube (8).

2. A mimicry sterile culture device for tobacco seedlings according to claim 1, wherein: every centre gripping subassembly (5) include slide rail (501), every slide rail (501) all are located in sliding tray one (202), every slide rail (501) top is equipped with slider (502), every surface mounting has electric telescopic handle (503) on slider (502), every movable block (504) are installed to the flexible end of electric telescopic handle (503), every all seted up in movable block (504) with one side outer wall communicating movable groove (505), every flexible movable groove (505) in all install servo motor one (506), every the output of servo motor one (506) is connected with threaded rod one (507), every threaded rod one (507) keep away from the one end of servo motor one (506) and are equipped with sleeve (508), every sleeve (508) other end wear out movable groove (505) and extend to external connection have grip block (509).

3. A mimicry sterile culture device for tobacco seedlings according to claim 2, wherein: each sliding block (502) is in sliding connection with a sliding rail (501), the outer wall of each sliding block (502) is in sliding connection with the inner wall of a first sliding groove (202), each sleeve (508) is in threaded connection with the outer wall of a first threaded rod (507), and each clamping plate (509) is in contact with the outer wall of a centrifugal tube (8).

4. A mimicry sterile culture device for tobacco seedlings according to claim 1, wherein: the material drawing component (4) comprises a hydraulic telescopic rod (401), the hydraulic telescopic rod (401) is fixedly connected with the upper surface of a fixed plate (7), the telescopic end of the hydraulic telescopic rod (401) penetrates through the lower surface of the fixed plate (7) and extends to a second placing groove (405) communicated with the second placing groove (404) of the second placing groove (405), a second through hole (404) communicated with one side outer wall is formed in the position, located on the first through hole (201), of the upper surface of the convex plate (402), circular grooves (403) communicated with the second through hole (404) are formed in the position, located on the first side outer wall and the second side edge, of the first syringe (9), of the first placing groove (403) are formed in the lower portion, a second spring (406) is arranged in the second placing groove (405), a second clamping block (407) is connected with the other end of the second spring (406), a first electric push rod (409) is arranged in the first groove (408) of the upper surface of the first protruding plate (402), a second electric push rod (409) is arranged in the first groove (408), a circular groove II (411) communicated with the lower surface and the outer wall at one side is formed in the transverse plate (410).

5. The pseudo-sterile culturing apparatus for tobacco seedlings of claim 4, wherein: the outer wall of each second clamping block (407) is in sliding connection with the inner wall of the second placing groove (405), and each second clamping block (407) is in contact with the outer wall of the injector (9).

6. A mimicry sterile culture device for tobacco seedlings according to claim 1, wherein: the displacement assembly (6) comprises two hydraulic rods (601), each hydraulic rod (601) is located in a lifting groove (103), each telescopic end of each hydraulic rod (601) is connected with a connecting plate (602), a second sliding groove (603) communicated with one side outer wall is formed in each connecting plate (602), a second servo motor (604) is mounted on one side inner wall of each second sliding groove (603), a second threaded rod (605) is connected to the output end of each second servo motor (604), a threaded plate (606) is sleeved on the outer wall of each second threaded rod (605), and a second fixing groove (607) is formed in each threaded plate (606).

7. The pseudo-sterile culturing apparatus for tobacco seedlings of claim 6, wherein: every hydraulic rod (601) bottom all with lift groove (103) inner wall bolt fastening, every connecting plate (602) outer wall all with lift groove (103) inner wall sliding connection, every threaded rod two (605) one end all with sliding tray two (603) inner wall rotation connection, every screw thread board (606) all with threaded rod two (605) threaded connection, every screw thread board (606) outer wall and sliding tray two (603) inner wall sliding connection.

8. A mimicry sterile culture device for tobacco seedlings according to claim 1, wherein: the utility model provides a sterile water tank (703) is installed to one side of alcohol case (701), aseptic water tank (703) is installed to one side of sodium chlorate case (702), the airtight link in alcohol case (701) bottom has flexible hose (704) and flexible hose (704) the other end through into fixed slot second (607) in displacement subassembly (6) in be connected with alcohol M pipe (7011), the airtight link in sodium chlorate case (702) bottom has flexible hose (704) and flexible hose (704) the other end through into fixed slot second (607) in displacement subassembly (6) be connected with sodium chlorate M pipe (7021), the airtight link in aseptic water tank (703) bottom has flexible hose (704) and flexible hose (704) the other end through into fixed slot second (607) in displacement subassembly (6) in be connected with sterile water M pipe (7031), protruding (402) in displacement subassembly (6) are all run through to outside under with the M end of sodium chlorate M pipe (7021) and sterile water M pipe (7031) in be connected with.

9. A mimicry sterile culture device for tobacco seedlings according to claim 1, wherein: the support (2) is located sliding tray one (202) below and all offered with communicating standing groove one (203) of through-hole one (201), every standing groove one (203) one side has been offered communicating fixed slot one (204), install electric putter (3) in fixed slot one (204), the flexible end of electric putter (3) is connected with grip block one (301), support (2) one end is connected and is equipped with two connecting rods (205), two connecting rod (205) outer wall cover is equipped with spring one (206), two the other end of connecting rod (205) runs through the opposite side outer wall of support (2) and extends to outside, one side that spring one (206) was kept away from to support (2) is located between connecting rod (205) is equipped with rotating electrical machines (1021), the output of rotating electrical machines (1021) is connected with arc stick (1022).

10. A mimicry sterile culture device for tobacco seedlings according to claim 9, wherein: the outer wall of the clamping block I (301) is in sliding connection with the inner wall of the placing groove I (203), two ends of the connecting rod (205) are fixedly connected with the inner wall of the drawer (102), the support (2) is in sliding connection with the outer wall of the connecting rod (205), the bottom end of the rotating motor (1021) is fixedly connected with the inner wall of the drawer (102) through bolts, and the arc-shaped rod (1022) is in contact with the outer wall of the support (2).