CN117224420A - Oil-soluble lip-rounding compound peptide and preparation method thereof - Google Patents

Abstract

The application discloses an oil-soluble lip-rounding compound peptide which is characterized by comprising the following components in percentage by weight: 0.1-1.0% of active polypeptide, 50-85% of oil phase matrix, 1-20% of emulsifier, 1-20% of auxiliary emulsifier, 1-15% of water, 0.1-1.0% of preservative and 0.01-0.1% of pH regulator, wherein the active polypeptide comprises a compound of lip-rounding/breast-rounding peptide, moisturizing peptide and anti-wrinkle and anti-aging peptide, and the lip-rounding/breast-rounding peptide is one or a combination of more of palmitoyl tripeptide-1, hexapeptide-3, palmitoyl isoleucine and acetyl hexapeptide-38; the moisturizing peptide is one or more of acetyl hexapeptide-37 and carnosine, and the anti-wrinkle anti-aging peptide comprises one or more of acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-38 and palmitoyl hexapeptide-12; the sum of the contents of all components is 100%.

Description

Oil-soluble lip-rounding compound peptide and preparation method thereof

Technical Field

The application belongs to the technical field of cosmetics, and particularly relates to an oil-soluble lip-rounding compound peptide and a preparation method thereof.

Background

The lips are important parts of the face, and are the transition parts from the oral mucosa to the skin, unlike facial skin, lip skin is not only free of sebaceous glands and sebaceous membranes, but also has extremely high metabolism rate of the stratum corneum, and the stratum corneum is thin so that immature cells are exposed on the surface of the lips. Therefore, the moisture of the lip skin is relatively easier to evaporate, and particularly when the movement or nasal obstruction needs to breathe with the mouth, the water loss rate of the lips is higher, and the lips are often dry, rough, dull and dull. The eastern aesthetic concept makes having a plump lip a new pursuit of many easterners.

It is also common in life to find that young women have lips that are full, free of lips, clearly visible to the volume of lip tissue, with age, thin and flat, senescent, flat, especially upper lips, with almost no complete lips visible, and very deep lips.

The ideal lip-beautifying standard is: the thickness of the center of the upper lip red lip is 7-8mm, the thickness of the center of the lower lip red lip is 10mm, the lip archwire is clear, the peak point of the lip is 3-5mm higher than the middle incision, the width of the split is 45-50mm for men and 42-50mm for women. With the development of medical plastic and scientific technology, lip-rounding technology is also more and more, and the lip-rounding method can be roughly divided into operation lip rounding, namely autologous fat particle transplantation, and non-operation lip rounding, namely injection lip rounding. However, these medical means have a non-negligible disadvantage: the operation means such as autologous fat particle transplantation not only has the risks of infection and the like in the operation, but also needs to take antibiotics and perform infection monitoring after the operation, and the price is high. The injection lip-rounding is low in risk, but cannot be injected too much, otherwise adverse reaction or infection phenomenon can occur, and the maintenance clearly aggravates the burden of consumers; lip balms or lip oils are often used by people to keep the lips moist, but far from the effect of filling the lips.

The prior art has the problems that:

1. most of bioactive polypeptides are hydrophilic macromolecules, and are often prepared into freeze-dried powder or aqueous solution for use, but most of lip care products are oily matrixes, so that the polypeptides are difficult to dissolve in oil, and the formula is difficult, and layering, precipitation, color change and other phenomena are easy to occur;

2. most of the bioactive polypeptides are formed by connecting specific amino acids through peptide bonds in a certain sequence, and are easy to hydrolyze and damage in aqueous solution, so that the activity is reduced;

3. the bioactive polypeptide has high hydrophilicity and high molecular weight, and although the lipophilicity is improved through hydrophobic groups such as palmitoyl, acetyl and the like, the bioactive polypeptide is still difficult to penetrate through a skin physiological barrier, has poor permeability and influences the efficacy;

4. the solubility of the hydrophilic active peptide in the oil phase is increased by adopting a conventional solubilization method, the solubilization effect is limited, and the oil solution with high peptide content is difficult to obtain. The water-in-oil microemulsion coating technology is adopted, so that the preparation difficulty is high due to the high surface tension of the internal water phase.

Disclosure of Invention

The application aims to: aiming at the problems and the defects existing in the prior art, the application aims to provide an oil-soluble lip-rounding compound peptide and a preparation method thereof.

The technical scheme is as follows: in order to achieve the above purpose, the present application adopts the following technical scheme: the oil-soluble lip-rounding compound peptide comprises the following components in percentage by weight:

0.1-1.0% of active polypeptide

50-85% of oil phase matrix

1-20% of emulsifying agent

1-20% of auxiliary emulsifier

1-15% of water

0.1-1.0% of preservative

pH regulator 0.01-0.1%

The active polypeptide comprises a composite of lip-rounding/breast-rounding peptide, moisturizing peptide and anti-wrinkle and anti-aging peptide, wherein the lip-rounding/breast-rounding peptide is one or a combination of more of palmitoyl tripeptide-1, hexapeptide-3, palmitoyl isoleucine and acetyl hexapeptide-38; the moisturizing peptide is one or more of acetyl hexapeptide-37 and carnosine, and the anti-wrinkle anti-aging peptide comprises one or more of acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-38 and palmitoyl hexapeptide-12;

the sum of the contents of all components is 100%.

Further, the oil phase matrix is a silicone oil, hydrocarbon oil or ester oil, and comprises at least two of polydimethylsiloxane, cyclopentadimethicone, squalane, isohexadecane, GTCC (caprylic/capric triglyceride), IPM (isopropyl myristate), 2EHP (ethylhexyl palmitate), pentaerythritol tetraisostearate, propylene glycol caprylic caprate, dioctyl carbonate and glycerol caprylate, and at least one of the oil phase matrix is an ester oil.

Further, the HLB value of the emulsifier is 5-9, and is one or more selected from polysorbate 80, polysorbate 20, span80 (sorbitan oleate), span20 (sorbitan laurate), polyglyceryl fatty acid esters such as polyglyceryl-3 diisostearate, cetyl polyethylene glycol/polypropylene glycol-10/1 dimethylsiloxane, PEG-20 glyceryl triisostearate, polyglyceryl-3 polyricinoleate and sorbitan isostearate.

Further, the co-emulsifier comprises ethylhexyl glycerol or glycerol octanoate.

Further, the preservative comprises one or more of phenoxyethanol, octanediol, 1,2 hexanediol, p-hydroxyacetophenone, cinnamon essential oil, lemon essential oil, clove essential oil and peppermint essential oil.

Further, the pH regulator is lactic acid or arginine for increasing the solubility of the alkaline polypeptide or the acidic polypeptide in the aqueous phase.

Further, the composition comprises the following components in percentage by weight:

ethylhexyl palmitate 56.875%

Squalane 20%

Span20 5%

Ethylhexyl glycerol 10%

PEG20 Triglycerol triisostearate 5%

Peppermint essential oil 0.005%

5% L-arginine aqueous solution 3%

And an active polypeptide comprising 0.025% palmitoyl isoleucine, 0.025% hexapeptide-3,0.025% palmitoyl tripeptide-38,0.025% acetyl hexapeptide-37;

the sum of the contents of all components is 100%.

The application also provides a preparation method of the oil-soluble lip-rounding compound peptide, which comprises the following steps:

step S1, weighing an oil phase matrix, an emulsifier, an auxiliary emulsifier and a preservative according to mass ratio, putting the oil phase matrix, the emulsifier, the auxiliary emulsifier and the preservative into a preparation pot, and stirring the materials to completely and uniformly mix the materials to obtain a transparent oil phase solution;

s2, in addition, weighing water according to the mass ratio, then adding a pH regulator, and stirring to completely dissolve to obtain a transparent aqueous phase solution;

step S3, adding active polypeptide into the aqueous solution obtained in the step S2, and stirring to completely dissolve the active polypeptide to obtain an aqueous solution containing the active peptide;

and step S4, slowly adding the water phase solution containing the active peptide obtained in the step S3 into the oil phase solution obtained in the step S1 under stirring, and keeping stirring for 1-2h to obtain the oil-soluble transparent microemulsion.

The beneficial effects are that: compared with the prior art, the oil-soluble lip-rounding compound peptide of the application adjusts the polarity of an oil phase mainly through a compound oil phase so as to improve the formation of microemulsion and facilitate the subsequent compounding with various oily matrixes; secondly, two emulsifying agents are compounded to enable the interface film to be more compact and firm so as to improve the stability of the microemulsion; thirdly, a three-dimensional network is formed by utilizing non-covalent crosslinking self-assembly of carboxyl-carboxyl, carboxyl-amino and the like among the active polypeptides, and the mode that a hydrophobic long chain is inserted into an oil phase to enable the microemulsion to be more stable is adopted, and a W/O type microemulsion encapsulation technology is adopted to obtain colorless to light yellow transparent liquid with high active peptide content, and the method has the following advantages:

(1) the product has high content of active peptide, colorless to pale yellow transparent liquid, can be mixed with various oily matrixes in any proportion when in use, does not delaminate, discolor or devitrify during storage, and can meet the needs of most customers.

(2) The microemulsion drop has small particle diameter (less than 100 nm), belongs to a thermodynamic and kinetic stable system, has high encapsulation efficiency and good stability, can increase the lipophilicity of hydrophilic active polypeptide, and is beneficial to the percutaneous permeation of macromolecular active polypeptide, thereby enhancing the efficacy of each peptide.

(3) The microemulsion encapsulation technology is adopted to encapsulate active polypeptides with different properties, so that not only can the stability of various polypeptides be increased, but also the release speed of the polypeptides can be regulated, and the purpose of sustained release is achieved.

(4) The product selects three active polypeptides with full lip-rounding, moisturizing, anti-wrinkle and anti-aging effects for compounding, and the three active polypeptides are compounded according to the optimal proportion aiming at different targets respectively, and the three active polypeptides are synergistic and have good lip-rounding effect after long-term use.

(5) The essential oil is compounded with polypeptide to play multiple roles of lip enlargement, moisturizing, natural preservation, taste correction and the like.

Drawings

FIG. 1 is a schematic view showing the appearance of the microemulsions prepared in examples 1 to 5 of the present application;

FIG. 2 is a schematic diagram showing a safety evaluation test of the oil-soluble lip-rounding compound peptide prepared in example 1 of the present application;

FIG. 3 is a graph showing the results of clinical tests of the oil-soluble lip-rounding compound peptide prepared in example 1 of the present application.

Description of the embodiments

The present application is further illustrated in the accompanying drawings and detailed description which are to be understood as being merely illustrative of the application and not limiting of its scope, and various modifications of the application, which are equivalent to those skilled in the art upon reading the application, will fall within the scope of the application as defined in the appended claims.

Except for the special description, the equipment used in the embodiment and the comparative example are conventional experimental equipment, the materials and the reagents used are all obtained in the market without the special description, and the experimental method without the special description is also a conventional experimental method.

Example 1: the preparation process of the oil-soluble Feng Chun compound peptide in the embodiment is as follows:

step S1, weighing 56.875% of ethylhexyl palmitate, 5% of PEG20 glycerol isostearate, 5% of Span20, 10% of ethylhexyl glycerol and 0.005% of peppermint essential oil, and stirring at the speed of 200 rpm to completely dissolve until the mixture is clear to obtain solution A;

step S2, respectively weighing 0.025% of palmitoyl isoleucine, hexapeptide-3, palmitoyl tripeptide-38 and acetyl hexapeptide-37 according to the mass ratio, and adding 5% of L-arginine water solution to stir for complete dissolution to obtain solution B;

and step S3, slowly adding the solution B into the solution A at the stirring speed of 200 rpm, and continuously stirring for 1h to obtain the finished product. The appearance, stability and dilution compatibility results are shown in Table 1.

Examples 2 to 5: comparative analysis of the Effect of different oil phases on microemulsion Performance

This embodiment differs from embodiment 1 in that: the microemulsion is prepared by adopting single grease or two grease compounds with different polarities as oil phases according to the prescription dosage of the table 1, and the influence of the formation and stability of the microemulsion with different oil phases is compared based on the transparent uniformity of the microemulsion appearance and the non-delamination of the microemulsion after standing for 24 hours at room temperature. And further the use of diluent oils (olive oil, squalane and GTCC) commonly used in cosmetics, according to microemulsion: oil = 1:10, and the compatibility is compared, and the results are shown in Table 1.

TABLE 1 influence of oils of different polarity on nanoemulsion quality

The result shows that: examples 1,2, and 5 all formed transparent to translucent uniform and stable W/O microemulsions, while examples 3 and 4 were milky-white in appearance, difficult to form transparent microemulsions, and significantly delaminated after 24h at room temperature, demonstrating that stable microemulsions were difficult to form with one non-polar oil phase cyclopentadimethicone or squalene alone (see FIG. 1). In the embodiment 2, the medium-chain fatty acid synthetic oil ester is used as an oil phase, so that the microemulsification performance is greatly improved, and the microemulsions can be formed; examples 1 and 5 are two different oil phases which are compounded to form transparent microemulsion, layering phenomenon does not occur after the transparent microemulsion is placed for 24 hours at room temperature, but example 5 adopts two kinds of grease with larger polarity to be compounded, and atomization is carried out after the grease is mixed with a common nonpolar oil phase in cosmetics, so that compatibility is poor, and therefore, the compounding stability and compatibility of the oil esters with two different polarities are better in the implementation 1.

Examples 6 to 10: comparative analysis of the Effect of different emulsifiers on microemulsion Performance

The present embodiment differs from embodiment 1 in that: the microemulsion was prepared according to the law by selecting different types of emulsifiers and the compound emulsifiers according to table 2, observing the appearance of the microemulsion, standing at room temperature and centrifugal stability, and evaluating the influence of different emulsifiers on the performance of the microemulsion, and the results are shown in table 2.

TABLE 2 influence of different emulsifiers on the quality of the microemulsions

As a result, it was found that the stability of the prepared microemulsions was slightly inferior in examples 6 to 8, and that examples 9 to 10 were each composed of two different emulsifiers, and that HLB was in the range of 5 to 9, and that the stability was significantly improved, and the inventors thought that the hydrophilic groups of Span-type emulsifiers were rigid ring-shaped structures and had a large volume, so that gaps were formed during the directional arrangement of the oil-water interface, and that the interfacial film was more dense and stable by adding a small amount of a secondary emulsifier containing hydrophilic polyoxyethylene chains, and the hydrophilic polyoxyethylene chains were inserted into the gaps, thereby improving the stability.

Therefore, the emulsifier with large oil affinity is particularly selected as a main emulsifier, and is compounded by being assisted with the emulsifier with certain hydrophilicity as a secondary emulsifier, and the HLB value is in the range of 5-9, so that the emulsifier has stronger affinity with the oil phase and also has certain affinity with the water phase.

Examples 11 to 15: comparative analysis of the Effect of different Co-emulsifiers on microemulsion Performance

This embodiment differs from embodiment 1 in that: the various co-emulsifiers were selected and the microemulsion was prepared according to Table 3, and the appearance, standing at room temperature and centrifugal stability of the microemulsion were observed, and the results are shown in Table 3.

TABLE 3 influence of different co-emulsification on quality of the nanoemulsion of the agent

The result shows that: in examples 11-12, conventional short-chain alcohol is used as an auxiliary emulsifier, and the appearance is transparent, so that microemulsion can be formed, but layering phenomenon appears after the microemulsion is placed for 24 hours at room temperature, and layering is more obvious after centrifugation, because the conventional short-chain alcohol and the conventional short-chain alcohol are hydrophilic short-chain alcohol, and the stability of W/O microemulsion is not facilitated. And examples 13-15 are all oil-soluble synthetic monoglyceride with certain hydrophilicity, have certain surface activity and good stability, and can be used as oil phase compound oil ester in a prescription, also can play a role of an auxiliary emulsifier and have optimal performance.

Examples 16-19 comparative analysis of the Effect of different active peptide formulations and different addition amounts on microemulsion Performance

This embodiment differs from embodiment 1 in that: the related properties were tested by compounding different active peptides and different aqueous phases and studied as shown in table 4.

TABLE 4 influence of different active peptides and amounts on microemulsion Properties

The result shows that: examples 16-17 were each formulated with water-soluble active peptides in different amounts to give clear and homogeneous microemulsions with no delamination after 24h standing, indicating that the properties of the microemulsions were not affected as long as the active peptides were completely dissolved in the aqueous phase. The formulation of example 18 contains palmitoyl pentapeptide-4 which is insoluble in water, and the problem of dissolution in water phase can be solved by adding a proper amount of acidic substances such as lactic acid and citric acid as pH regulator into water phase because it belongs to polypeptide of basic amino acid. Example 19 is a comparative example, and the formulation does not contain an active polypeptide.

Performance test: microemulsion stability, collagen production promotion and safety evaluation

(1) Stability investigation: the stability study was performed by selecting examples 1 and 19 (comparative examples) for acceleration test at 40℃at high temperature and 4℃at low temperature, -18℃and light (4500 LX) for 30 days and long-term test at 40℃and room temperature for 3 months, and the same concentration of aqueous solution was prepared as the amount of the active polypeptide of example 1 as a control to examine the appearance properties of the samples, demulsification and delamination and the marked amount percentage of the active polypeptide, respectively (wherein the samples at-18℃were left at room temperature after sampling and examined after melting), and the results are shown in Table 5.

TABLE 5 stability test results

As a result, it was found that the appearance properties of examples 1 and 19 (comparative examples) were not significantly changed by the low temperature of 4 ℃, -18 ℃ and the light conditions for 30 days and by the accelerated and long-term storage for 3 months, whereas example 19 (comparative example) was demulsified at ultra-low temperature of-18 ℃ and resulted in a small amount of delamination, and the analysis was due to the fact that the active polypeptide was a protein having carboxyl groups, amino groups and the terminal was modified with hydrophobic palmitoyl groups, acetyl groups, etc., not only the carboxyl groups, amino groups, etc., among the molecules of the active polypeptide were crosslinked in the form of hydrogen bonds, coordination bonds, etc., to form a stable three-dimensional network structure, but also the hydrophobic long chains thereof were further inserted into the oil phase through the interfacial film formed by the surfactant, making the interfacial film more compact and firm. Compared with the content measurement result of the active polypeptide aqueous solution with the same concentration in example 1, the stability of the active peptide is obviously improved, and further the oil-soluble polypeptide solution is further demonstrated to obviously improve the stability and be beneficial to the efficacy.

(2) Test for promoting collagen production

(1) Experimental materials: mouse embryo fibroblasts (NIH/3T 3), 10% new born calf serum+90% DMEM medium, PBS buffer, 0.25% pancreatin (EDTA), MTT, DMSO, and collagen ELISA assay kit.

(2) The experimental steps are as follows: NIH/3T3 cell fusion degree reaches about 80%, cell density is regulated by digestion with pancreatin, and the cells are uniformly inoculated into 96-well plates, and the number of cells in each well is 1×10 ⁴ Placing at 37deg.C and 5% CO ₂ After overnight incubation in the incubator, the old medium was blotted, 0.2mL of medium containing samples of example 1 at different concentrations was added to each well, three parallel controls were set, and a blank control was set up, and incubation was continued for 48h.48 After h, each cell is collected, and the influence of the sample on the collagen content of the NIH/3T3 cells is detected by adopting a collagen ELISA (enzyme-Linked immunosorbent assay) kit.

The results demonstrate that the oil-soluble lip-rounding compound peptide has a promotion effect on the collagen secretion of fibroblasts, the promotion rate reaches 21.5% when the concentration is 0.02 mg/mL, and the promotion effect shows concentration dependency (see Table 6).

TABLE 6 test results for promotion of collagen production

(3) Safety evaluation

(1) The subject: female 13, age range: 20-55 years old, test site: forearm medial, test period: 0.5h,24h and 48h after removing the plaque tester.

(2) The testing steps are as follows: the arms were first cleaned and rested for 20min in a constant humidity (40% -60%) environment at constant temperature (20 ℃ -25 ℃). Samples 0.020-0.025 g of example 1 were taken and added to the plaque assay chamber. The patch tester was applied to the forearm of the subject on the curved side, and gently pressed with the palm to uniformly apply the patch to the skin for 24 hours. Skin reactions were observed 0.5h,24h,48h after removal of the plaque tester, respectively, and the skin adverse reaction grading criteria are shown in Table 7.

TABLE 7 skin seal Patch test skin adverse reaction grading criteria

As a result, it was found that the skin closed patch test was negative for 13 subjects, and did not cause skin adverse reaction. The oil-soluble lip-rounding compound peptide has high safety, and a typical test photo is shown in figure 2.

Clinical application manifestation of lip enlargement

(1) Test sample: taking the product of the example 1, and diluting the product to the concentration of 5% by using jojoba seed oil to obtain lip-rounding oil;

(2) Test object: female volunteer 5 people aged 20-40 years;

(3) Test part: lips;

(4) Test period: 0d,7d,14d;

(5) Test instrument: moistureMeter SC (stratum corneum Water content tester), vapoMeter SWL5 (percutaneous Water loss tester), visioFace 1000D (facial image analyzer), and C-CUBE (multifunctional skin imaging System);

(6) The testing steps are as follows:

(1) cleaning lips, and resting for 20min in a constant-temperature (20-25 ℃) constant-humidity (40% -60%) environment;

(2) MoistureMeter SC and VapoMeter SWL 5) are adopted to measure the water content and the percutaneous water loss rate, visioFace 1000D) and C-CUBE) are adopted to measure the volume and depth of lips, and lip images are collected to measure the thickness of the convex part of the lips;

(3) the cleaning agent is used for cleaning and then is uniformly smeared on the lip part once in the morning and evening;

(4) legal test at 0d,7d,14d, respectively, and fill out a self-evaluation questionnaire after the test is completed.

Evaluation index and test result: the moisture retention performance and the volume and depth of the lips were measured for the moisturizing performance of the product, the lip thickness and the lip thickness were measured for the lip enlarging effect of the product, and the measurement results are shown in tables 8, 9 and 3.

Table 85% example 1 lip gloss clinical test results (n=5)

Table 9 subject self-evaluation questionnaire and results

From the test results of tables 8 and 9 and fig. 3, it can be seen that the oil-soluble lip compound peptide of the present application, after being applied 2 times per day, has a water content and a lip thickness which are significantly increased by 10.6% and 38.0% respectively for 7 days and 14 days, has a water content which is significantly increased by 4.0% and 9.3% respectively for the lip thickness, has a percutaneous water loss rate, a lip texture and a lip texture depth which are significantly reduced by 9.6% and 17.3% respectively for 7 days and 14 days, has a lip texture volume which is reduced by 8.1% and 20.3% respectively for the lip texture depth which is reduced by 5.4% and 12.1% respectively, and has a subject self-evaluation. The oil-soluble lip-rounding compound peptide has good transdermal absorption rate, can synergistically increase the effect, and has the comprehensive effects of moisturizing, reducing wrinkles and enlarging lips.

Claims (8)

1. The oil-soluble lip-rounding compound peptide is characterized by comprising the following components in percentage by weight:

0.1-1.0% of active polypeptide

50-85% of oil phase matrix

1-20% of emulsifying agent

1-20% of auxiliary emulsifier

1-15% of water

0.1-1.0% of preservative

pH regulator 0.01-0.1%

The active polypeptide comprises a composite of lip-rounding/breast-rounding peptide, moisturizing peptide and anti-wrinkle and anti-aging peptide, wherein the lip-rounding/breast-rounding peptide is one or a combination of more of palmitoyl tripeptide-1, hexapeptide-3, palmitoyl isoleucine and acetyl hexapeptide-38; the moisturizing peptide is one or more of acetyl hexapeptide-37 and carnosine, and the anti-wrinkle anti-aging peptide comprises one or more of acetyl hexapeptide-8, palmitoyl pentapeptide-4, palmitoyl tripeptide-38 and palmitoyl hexapeptide-12;

the sum of the contents of all components is 100%.

2. The oil-soluble lip compound peptide according to claim 1, wherein the oil phase matrix is a silicone oil, a hydrocarbon oil or an ester oil, and comprises at least two of polydimethylsiloxane, cyclopentadimethicone, squalane, isohexadecane, GTCC (caprylic/capric triglyceride), IPM (isopropyl myristate), 2EHP (ethylhexyl palmitate), pentaerythritol tetraisostearate, propylene glycol caprate caprylate, dioctyl carbonate, and glyceryl caprylate, and at least one of the oils is an ester oil.

3. The oil-soluble lip compound peptide according to claim 1, wherein the HLB value of the emulsifier is 5-9, and is a mixture of one or more of polysorbate 80, polysorbate 20, span80 (sorbitan oleate), span20 (sorbitan laurate), polyglyceryl fatty acid esters such as polyglyceryl-3 diisostearate, cetyl polyethylene glycol/polypropylene glycol-10/1 dimethylsiloxane, PEG-20 triisostearate, polyglyceryl-3 polyricinoleate and sorbitan isostearate.

4. The oil-soluble lip pencil peptide of claim 1, wherein the co-emulsifier comprises ethylhexyl glycerol or glycerol octanoate.

5. The oil-soluble lip rounding compound peptide of claim 1, wherein the preservative comprises one or more of phenoxyethanol, octylglycol, 1,2 hexanediol, p-hydroxyacetophenone, cinnamon essential oil, lemon essential oil, clove essential oil, peppermint essential oil.

6. The oil-soluble lip complex peptide according to claim 1, wherein the pH adjuster is lactic acid or arginine for increasing the solubility of the alkaline polypeptide or the acidic polypeptide in the aqueous phase.

7. The oil-soluble lip compound peptide according to claim 1, which is characterized by comprising the following components in percentage by weight:

ethylhexyl palmitate 56.875%

Squalane 20%

Span20 5%

Ethylhexyl glycerol 10%

PEG20 Triglycerol triisostearate 5%

Peppermint essential oil 0.005%

5% L-arginine aqueous solution 3%

And an active polypeptide comprising 0.025% palmitoyl isoleucine, 0.025% hexapeptide-3,0.025% palmitoyl tripeptide-38,0.025% acetyl hexapeptide-37;

the sum of the contents of all components is 100%.

8. A method for preparing the oil-soluble lip-rounding compound peptide according to any one of claims 1 to 7, which is characterized by comprising the following steps:

step S1, weighing an oil phase matrix, an emulsifier, an auxiliary emulsifier and a preservative according to mass ratio, putting the oil phase matrix, the emulsifier, the auxiliary emulsifier and the preservative into a preparation pot, and stirring the materials to completely and uniformly mix the materials to obtain a transparent oil phase solution;

s2, in addition, weighing water according to the mass ratio, then adding a pH regulator, and stirring to completely dissolve to obtain a transparent aqueous phase solution;

step S3, adding active polypeptide into the aqueous solution obtained in the step S2, and stirring to completely dissolve the active polypeptide to obtain an aqueous solution containing the active peptide;

and step S4, slowly adding the water phase solution containing the active peptide obtained in the step S3 into the oil phase solution obtained in the step S1 under stirring, and keeping stirring for 1-2h to obtain the oil-soluble transparent microemulsion.