CN1171965A - Tianjing Busuiye medicinal liquid and its preparing method - Google Patents
Tianjing Busuiye medicinal liquid and its preparing method Download PDFInfo
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Abstract
The present invention relates ot a compound medicinal preparation with the functions of supplementing five viscera (heart, lung, spleen, liver and kidney), regulating six pulses, supplementing qi and nourishing spirit, raising immunity of human body, engendering essence and boosting marrow and delaying senility and its preparation method. It is made up by using 25 Chinese and Western medicines and animal thymus gland mixture components, such as calf thymus gland, calf spleen, auricle of pig heart, ginseng, sea horse, epimedium, psoralea seed, scutellaria root, acanthopanax root, polygonatum root and procaine, etc. through such processes fo different pretreatment respectively, them proportioning, mixing, regulating pH value, measuring specific gravity, inspecting, sterilizing, reinspection and packaging.
Description
The invention belongs to the antiaging agent preparation, particularly relate to the pharmaceutical preparation that a kind of Tianjing Busuiye medicinal liquid kidney invigorating and YANG supporting improves immune function of human body, the invention still further relates to the manufacture method of Tianjing Busuiye medicinal liquid.
The people is to the decline gradually along with the growth at age of later on normal immunologic function of middle age, the immunologic function decline is the main cause of human senility, what come along with the immunologic function decline is to infect, the immunity comprehensive disease, the sickness rate of cancer obviously increases, and has quickened the human aging process, and these aging phenomenon traditional Chinese medical science are thought deficiency of the kidney, due to the brains deficiency,, benefiting essence and marrow controls antidotal theoretical foundation so just becoming dialectical opinion.At present, the medicine of some the kidney invigorating, health preserving, tonifying YANG is arranged also, the prescription of these medicines is had nothing in common with each other, and also is added with valuable wild animal medicine in the prescription, and these medicines also can tonifying kidney and preserving health, human body is played certain anti-ageing healthcare effect, but the medicine valency is expensive, and function is very not comprehensive.Seldom see the pharmaceutical preparation of the high treatment nephropathy of curative effect that Chinese medicine and western medicine prescription combination and animal thymus blending constituent utilize on the market.
The purpose of this invention is to provide and a kind ofly have kidney invigorating and YANG supporting, spermatogenesis benefit marrow, eliminate senile symptom, human body immunity improving function, benefiting essence and marrow pharmaceutical preparation of delaying senility function and preparation method thereof by what animal thymus blending constituent and Chinese medicine and western medicine prescription were formed.
The object of the present invention is achieved like this: the pharmaceutical preparation of kidney invigorating and YANG supporting delaying senility function, consisting of of Tianjing Busuiye medicinal liquid Chinese powder medicine component and each constituent content: calf thymus 8～30 grams, calf spleen 3～20 grams, auricle 3～25 grams on the pig heart, Radix Ginseng 3～15 grams, hippocampus 3～10 grams, Herba Epimedii 10～30 grams, Fructus Psoraleae 10～35 grams, Radix Scutellariae 10～30 grams, Rhizoma Polygonati 10～30 grams, Radix Et Caulis Acanthopanacis Senticosi 10～30 grams; Said medicine adjuvant, Radix Rehmanniae 10～30 grams, Fructus Lycii 10～30 grams, Radix Asparagi 10～25 grams, the Radix Astragali 10～30 grams, the Rhizoma Atractylodis Macrocephalae 8～25 grams, Radix Glycyrrhizae 8～30 grams, Radix Polygoni Multiflori 8～30 grams; Another ancillary drug Radix Paeoniae Alba 8～30 grams, Radix Codonopsis 8～30 grams, Placenta Hominis 6～30 grams, Herba Cistanches 8～30 grams, Radix Isatidis 8～30 grams ,/100 milliliters of procaine 0.0003～0.05 grams, Fructus Ligustri Lucidi 8～30 grams, Fructus Schisandrae Chinensis 8～30 grams.
A kind of pharmaceutical preparation Tianjing Busuiye medicinal liquid Chinese powder medicine component of kidney invigorating and YANG supporting delaying senility function and consisting of of each component optimum content: calf thymus 30 grams, calf spleen 15 grams, auricle 15 grams on the pig heart, Radix Ginseng 15 grams, Hippocampus 6 grams, Herba Epimedii 30 grams, Fructus Psoraleae 25 grams, Radix Scutellariae 25 grams, Rhizoma Polygonati 25 grams, Radix Et Caulis Acanthopanacis Senticosi 25 grams; Adjuvant Radix Rehmanniae 15 grams of above-mentioned principal agent, Fructus Lycii 20 grams, Radix Asparagi 15 grams, the Radix Astragali 20 grams, the Rhizoma Atractylodis Macrocephalae 15 grams, Radix Glycyrrhizae 20 grams, Radix Polygoni Multiflori 20 grams; Another group adjuvant Radix Paeoniae Alba 15 grams, Radix Codonopsis 25 grams, Placenta Hominis 25 grams, Herba Cistanches 20 grams, Radix Isatidis 20 grams ,/100 milliliters of procaine 0.0006 grams, Fructus Ligustri Lucidi 20 grams, Fructus Schisandrae Chinensis 20 grams.
The characteristics of animal thymus of the present invention are: the enhancing human body immunity function, the people is to middle age thymus atrophy gradually later on, become fatty tissue to geratic period thymus, among the human aging process, because the secretory function of thymosin descends, immunologic function also descends thereupon, make human body present aging phenomenon gradually, it is reported thymus transplantation in the human body triangular muscle, can treat immunodeficiency diseases, the human body immunity improving function.Thymosin is that the T cell individual is grown necessary, the forfeiture of T cell often causes autoimmune generation, for being tending towards old and feeble people gradually, extracts the mixing composition (containing the thymosin parahormone) of animal thymus, but the enhancing human body immunity function, and the effect of the senilism of preventing is arranged.There is not obvious species specificity according to newly organized " materia medica " third edition record thymus parahormone.
Spleen is the immune device of human body maximum, the cell that spleen produced has the effect of engulfing antibacterial, virus and harmful substance, the traditional Chinese medical science is thought with the dirty effect that the human body immunity improving function is arranged of dirty benefit, extracts the spleen liquid factor, and being used to strengthen immune function of human body is the theory that meets Chinese medicine.
The auricle of pig contains a kind of heart factor, for old and feeble gradually people, extracts the heart humoral factor, is applied to the irritability that human body can increase heart, improves cardiac function.
Have effects such as " tonifying five ZANG-organs ", " peace spirit " according to ancient book " herbal guiding principle wood ", " book on Chinese herbal medicine converges and says ", Shennong's Herbal record Radix Ginseng, above-mentioned calf thymus, calf spleen, three kinds of animal drugs of Cor Sus domestica ear are equipped with the merit that Radix Ginseng, Hippocampus, Herba Epimedii, Fructus Psoraleae, Radix Scutellariae, Radix Et Caulis Acanthopanacis Senticosi, Rhizoma Polygonati have YIN nourishing and YANG strengthening, heat-clearing and toxic substances removing, tonifying five ZANG-organs, peace spirit, root for the human body of bestirring oneself--kidney yang plays an important role with the gas of warm promotion whole body, but Hippocampus one medicine can see that disease can reach the function of blood circulation promoting competent silt and heat-clearing and toxic substances removing to common old and feeble blood stasis.The ancillary drug Radix Rehmanniae of this prescription principal agent, Fructus Lycii, Radix Asparagi, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, Radix Polygoni Multiflori have invigorating the liver and kidney promoting generation of vital essence and blood, Yin-nourishing and body fluid promoting, cooling blood for hemostasis, reducing the asthenic fever effect.This another group adjuvant Radix Paeoniae Alba of filling a prescription, Radix Codonopsis, Placenta Hominis, Herba Cistanches, Radix Isatidis, procaine, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis are the YIN nourishing of enriching blood, nourishing YIN and benefiting blood, the effect of calming the nerves.
The procaine medicine can alleviate the depressed and worried of elderly patients, improving memory and ability of thinking.Has the effect of mediator's somatic nerves function balance.
The preparation that this full formula compatibility is comparatively precise and appropriate has cooperative effect, has no side effect through the zoopery proof.Can replenishing vital QI with drugs of warm nature Rong Wei, tonifying five ZANG-organs, accent six kinds of pulses, grow fill hundred arteries and veins, moist three warmers, invigorate blood circulation help gas, reach that QI invigorating is reposed, the merit of YIN nourishing and YANG strengthening, heat-clearing and toxic substances removing and benefiting essence and marrow, and can human body immunity improving function, slow down aging play main effect, old people's deficiency in both YIN and YANG, QI and blood impassable patient are suited.
The main Chinese medicinal materials source is: Radix Ginseng is produced in northeast, Hippocampus is produced in Guangdong, the Herba Epimedii stem and leaf is produced in Shanxi, scutellariae,radix is produced in northeast, the Fructus Psoraleae fruit is produced in Sichuan, the compound preparation that 25 kinds of Chinese herbal medicine such as Xinjiang calf animal thymus, procaine, animal drugs, Western medicine are formed, drug dose are all stipulated by (pharmacopeia nineteen ninety-five first one).
This medicine is a yellowish-brown liquid, sweet, little hardship of distinguishing the flavor of, and every bottle 250 milliliters * 1 bottle, every milliliter contains crude drug in whole 1 gram.Oral one time 10 milliliters, a twice-daily, (medicine) being taken before meal is sooner or later taken medicine and is avoided strong tea in back 2 hours, if any precipitation, takes after shaking up, and curative effect is not had influence.Nontoxic, the side effect of this product.Want lucifuge, airtight, shady and cool place to preserve.
The manufacture method of above-mentioned Tianjing Busuiye medicinal liquid Chinese medicine in turn includes the following steps: selected first-class medical material clean → was pulverized 40 mesh sieves, take by weighing weight: Radix Ginseng 3～15 grams, hippocampus 3～10 grams, Herba Epimedii 10～30 grams, Fructus Psoraleae 10～35 grams, Radix Scutellariae 10～30 grams, Rhizoma Polygonati 10～30 grams, Radix Et Caulis Acanthopanacis Senticosi 10～30 grams; Radix Rehmanniae 10～30 grams, Fructus Lycii 10～30 grams, Radix Asparagi 10～25 grams, the Radix Astragali 10～30 grams, the Rhizoma Atractylodis Macrocephalae 8～25 grams, Radix Glycyrrhizae 8～30 grams, Radix Polygoni Multiflori 8～30 grams; The Radix Paeoniae Alba 8～30 grams, Radix Codonopsis 8～30 grams, Placenta Hominis 6～30 grams, Herba Cistanches 8～30 grams, Radix Isatidis 8～30 grams, Fructus Ligustri Lucidi 8～30 grams, Fructus Schisandrae Chinensis 8～30 gram mercerations bubble 24 hours → (the changzhou second chemical mechanical equipment factory makes to put into multi-function extractor, model 0N type 200V) water is carried 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 gram amount by every milliliter and add distilled water after quiet straight 24 hours → vacuum filtration → quiet straight 24 hours final vacuum sucking filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.Annotate: will contain the medicine of volatile oil, vacuum extraction volatile oil will extract in medicinal residues and the straight in the lump extraction pot of said medicine then in advance.The volatile oil that extracts stocked wait to join.
The animal drugs extracting method of Tianjing Busuiye medicinal liquid may further comprise the steps successively: with the calf thymus of getting ready, the calf spleen, on the pig heart auricle in advance 8～20 ℃ of one weeks of stored frozen to reach the purpose of handling autolytic ferment → remove connective tissue and impurity → clean → slurrying (rubbing) → take by weighing weight calf thymus 8～30 grams with electrical meat mincer with distilled water, calf spleen 3～20 grams, the normal saline of auricle 3～25 gram → adding doubling doses leaches on the pig heart, soak earlier and soaked filtration in 0.5 hour (can soak as the case may be 2～3 times) → made medicinal liquid slightly be chilled to 40～60 ℃ in 3～5 minutes the rapid heated and boiled of filtrate at 75～80 ℃ again in 1 hour and use 6.804 kilograms of pressure through 30 minutes heat treated immediately with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out with paper pulp cake sucking filtration → filtrate at room temperature, filter with frozen water cooling reuse paper pulp cake then, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, the Pu Lu point is because of/100 milliliters of adapteds of 0.0003～0.05 gram, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.
The excellent beneficial effect of Tianjing Busuiye medicinal liquid is that treatment is suffered from a deficiency of the kidney, the kidney invigorating, QI invigorating, help the effect of intensive culture god, YIN nourishing, tonifying YANG and heat-clearing and toxic substances removing.Improve human body reticuloendothelial system phagocytic antibacterial, the ability of virus and harmful substance through experiment confirm; Improve the function of human body cell inducement interferon; Nervous system there are balance and regulating action; The stem cell that bone marrow is produced is transformed into the T lymphocyte, thereby can strengthen and improve immune function of human body.Sick evident in efficacy for brain function decline, neurasthenia, the tinnitus of having a dizzy spell etc., memory reinforcing; Diseases such as sexual hypofunction, climacteric syndrome, removing lipofuscin (senile plaque), soreness of the waist and knees, rheumatoid arthritis, hepatitis also there is significant curative effect; Can promote fracture heals in early days, postcerebral injury syndrome, the patient who weakens for after the wound and operation back resistance is more remarkable.
Put to the proof below by relevant test:
One, slow down aging and the clinical observation of treatment insufficiency of kidney-YANG
Anti-old (energy) three essentials-essence theory and the Immunization Update theory paid attention to of inventor's relevant health preserving from ancient medical book (health preserving side), 25 kinds of Chinese medicine and western medicine, the animal drugs of screening, through clinical 26 years research, practice, utilize the refining compound preparation that forms of modern science technology.To 80 examples, the senile symptom patient that suffers from a deficiency of the kidney is divided into Tianjing Busuiye medicinal liquid group (treatment group) at random through clinical, sucrose solution placebo group (matched group), adopt the double blind control method, took medicine three months, observed result shows: Tianjing Busuiye medicinal liquid can make the total mark value decline (p＜0.01) of elder kidney asthenia senile symptom.Visual acuity and audition improve, and heart rate shortens between often again, and memory improves.Superoxide dismutase in the erythrocyte (SOD) vigor raises (p＜0.01), and blood plasma lipid peroxide (LPO) content descends; Comparing with matched group all has significant difference (p＜0.01).Prompting reduces the free radical poisoning injury, strengthens anti-oxidant vigor, originally can alleviate the total effective rate 94% of senile symptom.In order further to confirm its clinical effect, the same period, between year March nineteen ninety-five to 1997, we adopted the double blind control method, carry out second phase paired observation to there not being the elder kidney asthenia patient of serious organic disease, and acquisition data 80 examples now are reported as follows the result.
1, clinical data
The patient is from Wusu City, Xinjiang institute of traditional Chinese medicine, Yi Li continent Kuitun hospital and Yi Li continent Kuytun City hospital.In the second phases 80 example, male's 60 examples, women's 20 examples at 60～80 years old age, are divided (treatment group) 50 examples, mean age 68,11 ± 5,31 years old, sucrose solution placebo group (matched group) 30 examples at random.
2, the diagnostic criteria reference literature is formulated (1):
(1) geratic period 〉=60 year old, (2) are selected to look into (comprising electrocardio, hematuria routine, liver, renal function, blood pressure detection etc.) through visiting, prove be not in the mood for, organic disease persons such as brain, liver, kidney and endocrine.
3,14 senile symptoms are kept the score with the dynamic change classification: reference literature is formulated relevant all types of dialectical standards and the senile symptom integrated value evaluation criteria of suffering from a deficiency of the kidney in (1).Soreness of the waist and knees; Lassitude and weak; The cold syndrome of the stomach cold extremities; Nocturia frequently; Spontaneous perspiration; Breathe hard; Big loose stool is thin; Alopecia or poliosis; Forgetful; Luxated tooth; Hyposexuality; Tinnitus; Dizzy; Sleep not good enough or insomnia.
Possess insufficiency of kidney-YANG, the deficiency of kidney-QI binomial, in three of minor symptoms and the tongue arteries and veins one all scores according to integration method.
4, senile symptom integrated value assessment method: the symptom note that initiatively say (1) 4 minutes.(2) remarkable or lasting note 3 minutes that occur of the symptom of getting.Heavy or be interrupted the symptom note that occurs 2 minutes when light when (3) getting.(4) get gently or the symptom note that occurs once in a while 1 minute.(5) asymptomatic note is 0 minute.(6) any person of appearance all is designated as 6 fens in the tongue arteries and veins.
5, medicine and method of administration: reference literature is formulated (2), adopts the randomized controlled method, and oral Tianjing Busuiye medicinal liquid 20ml/ day is organized in treatment, divides empty sooner or later clothes respectively to obey 10ml.Matched group clothes sucrose solution placebo, dosage and the method same with the treatment group, each group serve on three months.
6, health check-up method:
(1) intelligence is measured: comprise and point to memory and learning by association, 1. point to note second testing method: after the experimenter listened to the content in the recorder and requires, it at first answered out " sensings " content (120s prescribes a time limit).Answer out non-" sensing " content (120s in limited time) again.The standard of keeping the score: whenever answered questions a speech note 1 minute, and answered wrong and answer button 1 minute, inerrancy full marks (pointing to and non-sensing) respectively 12 minutes more.2. the testing method of learning by association: listen to capstan, the standard of keeping the score is, " answers " two kinds with " answering difficulty " in the problem of answering easily, whenever answers questions a speech of " easily " and remembers 0.5 fen, whenever answer questions speech note 1 minute of " difficulty ", answer be 9 minutes to full marks entirely.
(2) look adjusting power (visual acuity): for carrying out after the calibrated vision of ametrope, write down the myopia point and the High eye point of images of left and right eyes respectively, calculated difference is visual acuity (cm).
(3) audition: respectively from 250Hz, 4000Hz, the ascending and descending adjusting input of signal generator ab amount can the amount of listening be that audition value (dB) is calculated with minimum ab amount.
(4) postexercise heart rate assay method recovery time: squat upright movable 10 times to 20 times, write down its postexercise heart rate required time (S) when returning to normal cardiac rate respectively.
(5) the one retraction time of skin pull-up: inboard 3cm is the trial zone on the former wrist: let go after 1 second with thumb, forefinger pull-up skin, write down it with stopwatch and calm down the time (S).
7, lab testing: (1) routine blood test; (2) routine urinalysis and glucose in urine are qualitative; (3) liver function; (4) serum urea nitrogen (Buw).(5) plasma lipid peroxide (LPO).(6) surpass oxide enzyme (SOD) in the erythrocyte.(7) glutathione peroxidase (GSH-Px) is measured in the whole blood; LPO adopts Yagi spark gap state husband method (3), and SOD adopts the nitrite forming method, and GSH-Px adopts the Hafeman method.
The result
8, efficacy assessment standard: reference literature is formulated (1).
(1) produce effects: 1. treatment back aging sees that the disease integration descends more than 2/3; 2. take a turn for the better more than 2 in 1～5 of the health check-up.3. 3 improvements in 4～7 of the lab testings.
(2) effective: 1. treatment back aging sees that the disease integration descends 1/3.2. 2 improvements in 1～5 of the health check-up.3. 2 improvements in 4～7 of the lab testings.
(3) invalid: as fail to reach above-mentioned effective standard, or lab testing to have 2 (and more than 2) persons of increasing the weight of.
9, two groups of curative effects relatively see Table 1:
Table 1 liang group curative effect relatively
| The group example is not several | Produce effects | Effectively | Invalid | Effectively total |
| Example number % | Example number % | Example number % | ????％ | |
| 50 matched groups 30 are organized in treatment | ?22??44 ?0???0 | ?25????50 ?8?????26.7 | ?3?????6 ?22????73.3 | ????94 ????26 |
Behind the treatment group clothes Tianjing Busuiye medicinal liquid, its aging sees that the disease integration analyzes through Ridit, all significantly reduces p＜0.01).And difference is not remarkable before and after the matched group placebo treatment.
10, physical examination results change:
(1) no significant change before and after the treatment of blood pressure, pulse treatment group, illustrate this product to nothing obviously influence.
(2) intelligent measurement result: the treatment group is pointed to has highly significant to improve (p＜0.01) after memory is treated with learning by association, and no significance changes before and after the treatment of control group.
(3) visual accommodation (visual acuity) result: treatment group left eye (p＜0.01) right eye (p＜0.05).And matched group does not have significant change.
(4) audition treatment back result: left and right ear significantly improves (p＜0.01) before treating, and matched group does not have significant change.
(5) postexercise heart rate measurement result recovery time: heart rate has obvious shortening (p＜0.01) between often again before and after upright movable 10 to 20 the treatment groups treatment of squatting.And matched group has the prolongation of different time, but the statistics there was no significant difference.
(6) skin pull-up retraction time result: two groups of treatment back differences compare (p＜0.01), illustrate that treatment group treatment back skin elasticity strengthens, and matched group is no change then.
11, blood, routine urinalysis and hepatic and renal function check result: the treatment front and back are all in normal range.Part patient has surveyed, cholesterol, blood glucose, equal no change before and after taking medicine.
12, blood plasma LPO before and after the treatment, SOD in the erythrocyte, blood GSH-Px changes: the treatment group, compare before and after the treatment, blood plasma LPO content decline (p＜0.01), erythrocyte sod is active to rise (p＜0.01), and more all has significance to change (p＜0.01) according to group, no significance variation before and after the treatment of control group.
Table 2 liang group blood plasma LPO content, erythrocyte sod, the comparison of blood GSH-Px vigor (xx ± S)
| Group | The example number | ???LPO(mmol/ml) | ????SOD(ug/g.Hb) | GSH-PX (unit of activity) |
| The treatment group | Treat preceding 50 treatment backs 49 | ???4.49±0.71 ???3.27±0.52※ | ???488.51±46.46 ???571.35±95.54※ | ???58.82±8.02 ???61.95±6.62 |
| Matched group | Treat preceding 30 treatment backs 26 | ???4.48±0.72 ???4.23±0.62 | ???489.89±57.40 ???503.35±43.78 | ???61.09±8.60 ???62.93±7.14 |
Annotate: with preceding relatively ※ p＜0.01 of treatment; Compare Δ p＜0.01 with matched group.
13, side effect: do not find any side effect after the treatment.
Conclusion
The traditional Chinese medical science is thought, kidney controlling essence storage, and with people growth, grow old and feeble closely relatedly, kidney qi is contained the then longevity, and kidney qi declines and then dies young.Therefore the kidney invigorating is an important channel of life lengthening, and this paper is discussed below its effect by the clinical observation to Tianjing Busuiye medicinal liquid:
(1) these product can make the aging of suffering from a deficiency of the kidney see that the disease integrated value obviously descends, and are especially improving aspect the insufficiency of kidney-YANG pattern of syndrome more significantly, illustrate that this side has slow down aging, the kidney invigorating effect.
(2) the old and feeble patient that suffers from a deficiency of the kidney takes that SOD obviously raise when LPO descended in blood behind the Tianjing Busuiye medicinal liquid, illustrates that these product reduce the effect of radical pair body damage, may be by improving the vigor realization of peroxidase.
(3) these product can improve brain intelligence, memory reinforcing, and the raising of visual acuity and audition, heart rate shortens between often again.
To sum up every: the effect that Tianjing Busuiye medicinal liquid has the kidney invigorating and removes old people's senile symptom, have slow down aging, remove the effect of free radical.
List of references
1, Chen Guiting, etc., up-to-date domestic and international disease treatment standard, Xueyuan Press, 1 edition, Beijing, 1991:1416,1418 (the screening rules of slow down aging Chinese medicine and clinical observation standards).
2, Du Xin, etc., vigor Soviet Union anti-aging effects research-507 routine double blind control methods are observed, combination of Chinese and Western medicine magazine, 1986,6 (5): 271.
3, Yagi spark gap state husband, serum is crossed the clinical meaning of acidify lipid assay と body, clinical pathology, 1981,29 (3): 221.
Two, the clinical observation of Tianjing Busuiye medicinal liquid slow down aging
To 42 examples, the clinical observation result of healthy elderly shows through clinical:
(1) can obviously increase the total E rosettes of old people (ETRFC) percentage rate (P＜0.01) and T--lymhocyte transformation rate (P＜0.05).Has the effect that improves the body ability to work and eliminate old symptom.
(2) have the peripheral leukocytes sum of increasing and neutrophil cell (P＜0.01),, promote the effect of cellular immunization and humoral immunization, slow down aging is played one of main mechanism to strengthen specific immunity.
Object and method
1, the selection of object: the patient is from Wusu City, Xinjiang institute of traditional Chinese medicine, Yi Li state, Xinjiang Kuitun hospital, Yi Li state, Xinjiang Kuytun City hospital, by (Gerontological Society of Chinese Medical Association required standard (1)), selection proves through clinical examination (comprising inspections such as electrocardio, hematuria routine, liver function, kidney merit, blood pressure) and is not in the mood for, brain, lung kidney and in organic disease person's 42 examples such as branch oozes, male's 27 examples wherein, women's 15 examples, 60～80 years old age, and the mean age 68.03 ± 5.31 (x ± SD).Be divided into Tianjing Busuiye medicinal liquid group (treatment group) 36 examples at random, sucrose solution placebo group (matched group) 6 examples.
2, take medicine and Therapeutic Method: adopt the double blind control method, reference literature (2～4), oral Tianjing Busuiye medicinal liquid 20ml/ day is organized in treatment, divides sooner or later each 10ml on an empty stomach.Matched group clothes sucrose solution placebo, dosage and the method same with the treatment group, each organizes one month is a course of treatment, observes two courses of treatment altogether.Tianjing Busuiye medicinal liquid and sucrose solution placebo provide by the little pharmaceutical factory of Wusu City, Xinjiang institute of traditional Chinese medicine.
3, observation index: comprise clinical symptoms, physical examination, generally chemical examination and test on immune function.
(1) clinical symptoms: giddy, fatiguability, uncomfortable in chest, five symptoms relevant such as sleep is not good enough, inappetence with aging.
(2) general chemical examination: comprise white blood count and differential, routine urinalysis regulating liver-QI kidney function test.
(3) immunologic function test: comprise determination of immunoglobulin, the lysozyme quantitative assay is marked thymidine (3H-Tdk) with tritium and is mixed algoscopy and carry out lymphocyte transformation test and total E rosette.
The result
1, clinical symptoms change: the treatment group still has tired person's 2 exceptions after the treatment of clothes Tianjing Busuiye medicinal liquid, all the other symptoms all disappear, and behind the matched group clothes sucrose solution placebo, symptom does not have obvious improve (table 3).
Clinical symptoms before and after table 3 treatment relatively
| Group | ??n | Dizzy | Fatiguability | Uncomfortable in chest | It is not good enough to sleep | Inappetence | |
| The treatment contrast | After treating before treating after treating before treating | ??36 ??36 ??6 ??6 | ???14 ????0 ????3 ????0 | ????7 ????2 ????2 ????0 | ????7 ????0 ????2 ????0 | ????9 ????0 ????1 ????0 | ????3 ????0 ????0 ????0 |
2, the variation of numeration of leukocyte and classification: the total white blood cells of treatment group and neutrophil cell absolute number all are significantly increased after treatment.
Peripheral leukocytes comparison before and after table 4 treatment (x ± SD)
| Group | ??n | Numeration of leukocyte * 10 9/L | The neutrophil cell absolute number | |
| The treatment contrast | Treat before treating and treat back P value before back P value is treated | ??36 ??36 ??6 ??6 | ???6.700±1.734 ???8.224±1.582 ???????＜0.01 ???5.725±4.170 ???6.650±1.271 ???????＞0.05 | ????3784±1438 ????5034±1503 ???????＜0.01 ????3817±1290 ????3868±1456 ???????＞0.05 |
Immunoglobulin comparison before and after table 5 treatment (x ± SD)
| Group | ?n | ????IgG(g/L) | ??IgA(mg/L) | ?Igm(mg/L) | |
| The treatment contrast | Treat before treating and treat back p value before back p value is treated | ?36 ?36 ?6 ?6 | ??11.44±2.87 ??14.55±2.28 ??????＜0.01 ??10.28±1.99 ??16.03±0.69 ??????＜0.01 | ??970±450 ??930±690 ????＞0.05 ??970±400 ??1600±530 ????＜0.05 | ?1070±330 ?830±230 ???＜0.01 ?1070±270 ?1120±210 ???＞0.05 |
(p＜0.01)。And matched group behind clothes sucrose solution placebo except that sum slightly increases, the neutrophil cell absolute number does not have significant change (table 4).
3, liver, renal function change: treatment group and matched group are in the treatment liver function, and except that icteric index and thymol turbidity test slightly changed, all the other two (GPT and HBSAg) all belonged to normal or negative, the renal function and the front and back no change of taking medicine.
4, immunoglobulin changes: the IgG of treatment group its measured value after treatment obviously raises (p＜0.01), and IgA and IgM then do not have significant change; But in the matched group, two measured values of IgG and IgA also have significantly and increase, and Igm does not have significant change (table 5).
The variation of table 7 lysozyme: in treatment group and matched group.Bacterium enzymatic determination value after the treatment all has decline, but not statistically significant (table 6).
Lysozyme comparison before and after table 6 treatment (x ± SD)
| Group | ?n | Lysozyme is (mg/L) relatively | |
| The treatment contrast | Treat before treating and treat back P value before back P value is treated | ?25 ?25 ?4 ?4 | ????7.27±2.50 ????5.93±2.14 ?????＞0.05 ????7.60±3.69 ????4.18±1.42 ?????＞0.05 |
Lymphocytic comparison before and after table 7 treatment (x ± SD)
| Group | ??n | ????3H--TdK(SI) | |
| The treatment treatment | Treat before treating and treat back p value before back p value is treated | ??25 ??25 ??4 ??4 | ????62.77±36.29 ????100.63±71.12 ???????＜0.05 ????59.21±42.20 ????52.30±34.19 ???????＞0.05 |
5, the variation of lymphocyte transformation test: the stimulation index (Index ofstimulation be called for short SI) of treatment group after treatment obviously increases (p＜0.05), and matched group no change (table 7).
6, the comparison of total E rosette: treatment is organized total E rosettes measured value and is obviously increased (p＜0.01) matched group and then do not have significant change (table 8).
ETRFC comparison before and after table 8 treatment (x ± SD)
| Group | ??N | ???ET--RFC(％) | |
| The treatment treatment | Treat before treating and treat back P value before back P value is treated | ??25 ??25 ??4 ??4 | ???0.36±0.07 ???0.50±0.08 ?????＜0.01 ???0.42±0.06 ???0.47±0.05 ?????＞0.05 |
7, the back side effect of taking medicine: side effect takes place at the period in a medicine and none example of back of taking medicine.
Conclusion
Aging is the basic biological phenomenas of all lived individualities in the life evolution, by molecular biological research, thinks, the functional status of immuning tissue and immunocyte may be an old and feeble key factor of influence.By the clinical observation of Tianjing Busuiye medicinal liquid, its effect is discussed below:
1, improve the defying age ability of body: old and feeble clinical symptoms, this group have 40 examples time, in treatment back 2 middle of the month, 38 example time complete obiterations (95%) are arranged.
2, to the influence of nonspecific immunity: numeration of leukocyte and the neutrophil cell absolute number of treatment group after treatment has obviously and increases (P＜0.01) matched group and then do not have obvious variation.Be expected this medicine radioactivity, chemical, medicine and the caused leukopenia of other reason are had certain curative effect.
3, to the influence of specific immunity:
(1) influence of pair cell: the conversion test of T lymphocyte, the stimulation index of treatment group after treatment significantly increases (P＜0.05), and matched group does not then have significant change.In addition, total E rosette proves, treatment group its measured value after treatment also obviously rise (P＜0.01) prove that Tianjing Busuiye medicinal liquid has the effect of promotion cellular immune function.
(2) to the effect of humoral immune function: the main effect of B cell is to produce immunoglobulin.Observed result shows that Tianjing Busuiye medicinal liquid can make the measured value of IgG significantly increase (P＜0.01), illustrates that Tianjing Busuiye medicinal liquid has the function that strengthens humoral immunization by the B cell.
To sum up every, Tianjing Busuiye medicinal liquid can be used as slow down aging, and immune adjuvant is applied to clinical.
List of references
(1) Yang Zeyan, etc., several immune indexes measurement results of healthy elderly.China's geriatrics magazine 1984; 3 (4): 193.
(2) Zhou Wenquan, etc., the screening rules of slow down aging Chinese herbal medicine and clinical observation standard.Combination of Chinese and Western medicine magazine.1986；6<11>：682。
(3) Zhou Shangren compiling: immunodeficiency diseases, 1 edition, Tianjin science tech publishing house; Tianjin, 1981,367.
(4) Du Xin, etc., the anti-aging effects research of vigor Soviet Union--507 routine double blind control methods are observed.Combination of Chinese and Western medicine magazine.1986；6(5)：271。
Three, Tianjing Busuiye medicinal liquid is to benefiting essence and marrow, YIN nourishing, tonifying YANG pharmacodynamics research report
The compound recipe Chinese-western medicine preparation Tianjing Busuiye medicinal liquid of nourishing kidney yin and warming and recuperating the kidney-YANG is grown the sex character of castrating cock all optimum effect.And the observation of some leading indicators is shown nourishing kidney yin and the kidney yang sex hormone level in the body that is significantly increased, the mensuration of periphery physiochemical indice is significantly improved.
Select Tianjing Busuiye medicinal liquid the kidney invigorating side medicine for use, the internal relation of the theoretical material base of the pharmacodynamic action of research nourishing kidney yin and warming and recuperating the kidney-YANG and the traditional Chinese medical science " kidney storing essence, living marrow ".
We are model with the castrating cock, are promoting castrating cock sex character to grow, hematosis, and the mechanism that improves hemoglobin, and the observation of contrasting of aspects such as drug effect after effect, preliminary report of results is as follows.
1, materials and methods: (1) cock source: buy 80 days healthy male leghorn of brood birth 25 (average weight 85og) in May, 1996 from breed center, North SinKiang, Wusu City, Xinjiang and take action the quality testing epidemic disease after 1 week through this city veterinary station, the row male castration.
(2) castrating cock operation method: between the free rib of cock back 1-2, cut long mouthful of 2-3cm, separate the abdominal cavity mucosa, sew up after taking out a side testis, allow it recover to take out the opposite side testis with method after 5 days.Pathological section turns out to be the testis tissue of undeveloped mature.The half-castrate person of cutting only takes out one side testis.
(3) experiment administration group and medication: cock castrating back the 40th day, the cock that will meet complete castration requirement is divided into Tianjing Busuiye medicinal liquid (the kidney invigorating group) and castrating matched group (claiming full castrating group) at random.And get brood uncastrated cockerel in addition as the normal control group.Every group of 5-7 only.
The kidney invigorating group, the Tianjing Busuiye medicinal liquid adult who is provided by the little pharmaceutical factory of Wusu City, Xinjiang institute of traditional Chinese medicine measures 20 times of feed cocks, successive administration 60 days.Normal group and cut group entirely and will not administration handle, each conventional raising 60 days.
2, observation index: observed respectively and the preceding cock body weight of administration, cockscomb height, surface area and external appearance characteristic in 1-2 month after the administration.Administration 2 months and drug withdrawal were done the peripheral hemogram analysis respectively in 2 months, and did serum sex hormone level and measure the hemoglobin group analysis.
3, experimental result:
(1) castrating cock surface changes: castrate the back cock after routine is raised 39 days, and the obvious atrophy of cockscomb, formalness and behavior characteristics are obviously feminized, its body weight, cockscomb surface area and the percentile variation of cockscomb high growth see Table 9.
Each group of table 9 experiment back is hung down cockscomb surface area rate of increase than (m ± SD)
| Group (only) | Body weight (%) | Cockscomb surface area (%) | Cockscomb height (%) |
| Castrate group 16 half-castrate groups 5 normal group 4 entirely | ???64.1±22.1 ???64.8±25.3 ???51.9±19.5 | ?????-16.8±27.5※ ???????71.4±4.20 ??????101.8±48.3 | ???-1.2±22.9※ ???26.6±18.5 ???48.1±20.0 |
Annotate: ※ and normal group be P＜0.01 relatively.
(2) cock sex character is grown and the variation of surface after the administration: after the administration 30 days, 60 days, and the variation of surveying body weight, cockscomb height, cockscomb surface area and surface respectively.The result: after the administration cock body weight with castrate relatively no significant difference of group entirely.See Table 10 and table 11 show.
Respectively organize relatively (m ± SD) of cockscomb height change after table 10 administration
| Group (only) | Before the administration (cm) | After the administration 30 days (cm) | After the administration 60 days (cm) |
| Castrate group 5 the kidney invigorating groups 7 normal group 4 entirely | ???3.30±0.69 ???3.73±0.46 ???5.07±0.25※ | ???3.74±1.38 ???6.01±0.68※ ???7.43±0.78※ | ???4.18±1.11 ???7.20±0.68※ ???8.75±1.46※ |
Annotate: 60 days several 5 of detection chickens (table 11 together) after the administration of the kidney invigorating group.
※ with castrate relatively P＜0.01 of group entirely.
Respectively organize (m ± SD) of cockscomb surface area ratio after table 11 administration
| Group (only) | Before the administration (cm) | After the administration 30 days (cm) | After the administration 60 days (cm) |
| Castrate group 5 the kidney invigorating groups 7 normal group 4 entirely | ???8.92±1.62 ???13.89±2.75 ???25.76±3.87※ | ????9.45±5.17 ???33.79±3.97※ ???47.49±10.08※ | ????13.26±4.75 ????39.51±23.86※ ????72.94±13.19※ |
※ with castrate relatively P＜0.01 of group entirely.
Table 12 is taken medicine and was respectively organized the peripheral blood index in 60 days and change (m ± SD)
| Group (only) | Hemoglobin (g%) | Reticulocyte (%) | RBC number (%) |
| Castrate group 5 the kidney invigorating groups 7 normal group 4 entirely | ????15.23±0.92 ????13.58±4.20 ????17.06±1.20※ | ??0.40±0.49 ??1.16±0.68 ??2.00±1.40 | ??5.62±0.45 ??5.35±0.81 ??6.84±1.40 |
Annotate: with castrate relatively P＜0.05 of group entirely.
The growth of 30 days the kidney invigorating group cockscomb height and surface area all is higher than and castrates group (P＜0.01) entirely but more remarkable in the growth of administration cockscomb after 60 days after the administration.
The variation of cock surface shows: the kidney invigorating group disposition is active, and is how moving, identical with normal group, castrates group entirely and then have a sweet temper torpescence.The kidney invigorating group feather light, plentiful; It is loose to castrate the group feather entirely, tarnish.The kidney invigorating group cockscomb is upright plump the same with normal group scarlet.Castrate group cockscomb atrophy pinkiness entirely, behavior exactly likes hen especially.
Table 13 drug withdrawal was respectively organized the peripheral blood index in 60 days and is changed (m ± SD)
| Group (only) | Hemoglobin (g%) | Reticulocyte (%) | RBC number (%) |
| Castrate group 5 the kidney invigorating groups 7 normal group 4 entirely | ??14.40±1.90 ??12.74±3.60 ??18.30±1.88 | ??1.60±1.35 ??1.40±1.49 ??3.25±7.03 | ??5.83±0.92 ??4.29±1.37 ??7.03±0.65 |
Annotate: P＜0.05.
(3) to the influence of gonadal hormone: each organizes after the administration 45 days clear testosterone of extracting vein blood (T) respectively, estradiol (E2).The result: the kidney invigorating group serum T and E2 content increase.But no statistical significance (the P value all＞0.05).
(4) to the influence of periphery physiochemical indice: administration 60 days and drug withdrawal are measured respectively in the time of 60 days and are respectively organized cock periphery hemoglobin, reticulocyte, and red, leukocyte count, testing result sees Table 12, table 13.
Above results suggest: why Tianjing Busuiye medicinal liquid the kidney invigorating effect can improve hemoglobin content may be relevant with its hemopoietic function improvement.
Conclusion
The traditional Chinese medical science is thought people's life, length, strong, old and kidney is closely related, the people mostly is physiological " kidney " void to old age, the existing insufficiency of kidney-YANG of male's multilist, sexual disorder, the women then mostly is the ischemia of suffering from a deficiency of the kidney, and endocrine dysfunction makes each hypofunction of body, presents aging phenomenon, the kidney being the origin of congenital constitution, the root of life.Therefore utilize the medicine of benefiting essence and marrow, YIN nourishing, tonifying YANG, Tianjing Busuiye medicinal liquid is that specific action is arranged.
Selecting the castrating cock is model, the obvious atrophy of cockscomb after the cock castration, and feather is loose matt, and behavior is blunt, and outward appearance is obviously feminized, and presents " suffering from a deficiency of the kidney " ischemic state.The cockscomb growth is one of outstanding feature of cock sex character growth.Therefore, from the sex character variation of formalness, can intuitively reflect the partial action of medicine.Experiment shows: the initial stage of taking medicine is fast with the growth of (the kidney invigorating group) cockscomb of kidney invigorating and YANG supporting, and is tall and big upright, plump, scarlet, with the castrating matched group very notable difference arranged, again administration after 60 days above-mentioned situation more obvious.60 days contrast difference are not remarkable after drug withdrawal.
From the peripheral hemogram analysis result: the kidney invigorating group all has and increases.After the drug withdrawal 60 days, the kidney invigorating group peripheral hemogram was still kept higher level.Kidney invigorating and YANG supporting is described, the Tianjing Busuiye medicinal liquid medicine is lasting, and the after effect time is long.Prompting has the castrating of promotion cock sex character to grow and hematosis, so adopt benefiting essence and marrow, the kidney invigorating, tonifying YANG, more is tending towards " effecting a permanent cure ".Hormonal readiness is measured and is shown, the kidney invigorating, tonifying YANG, and the modulability hormonal action promotes the sex character developmental effects.
In sum, this medical instrument has benefiting essence and marrow and grows effects such as sun, tonifying YANG.The energy sex hormone level regulation promotes sexual function effect and function in delaying senility.
Four, the acute toxicity test of Tianjing Busuiye medicinal liquid
1, materials and methods: get 20 of the health large ear rabbits of 1.6-2.2kg, male and female half and half are divided into four groups at random.
The sorting table of 20 animals of table 14 during by four groups of random assortment
| Numbering | 1??2??3??4??5??6??7??8??9??10?11?12?13?14?15?16?17?18?19?20 |
| Random digit | 26?08?73?37?32?04?05?69?30?16?09?05?88?69?58?28?99?93?22?53 |
| Remove remainder with 4 | 2??0??1??1??0??0??1??1??2??0??1??1??0??1??2??0??3??1??2??1 |
| Distribute group | 24 one by one 44 one by one 24 one by one 41243121 |
Illustrate: remove each random number with group several 4, write down remainder.Pressing the big young pathbreaker animal of remainder divides to each group.Remainder is 1, is about to this animal and belongs to 1 group, and 2 return 2 groups, and 3 return 3 groups, and 0 returns four groups.
The grouping of table 15 completely random
| One group | 3??4??7??8??11?12?14?18?20 |
| Two groups | 1??9?15?19 |
| Three groups | 17 |
| Four groups | 2?15??6??10??13??16 |
Annotate: each treated animal is unequal, gets 4 numbers and remove first number with first group 9 earlier on random table, and 8 remove second number, and 7 remove the 3rd number, and 6 remove the 4th number.The gained remainder be respectively 5,5,3,4 arrange in order in being about to first group the 5th, and the like.
20 animal completely randoms of table 16 distribution result
| Group | Number of animals |
| First group | ?3??4??14??18??20 |
| Second group | ?1??9??15??19???6 |
| The 3rd group | ?17?11?12??7????8 |
| The 4th group | ?2??15?10??13??16 |
Annotate: grouping back animal difference, sub-cage rearing
4 groups of white rabbits are taken out the Tianjing Busuiye medicinal liquid that gavages 4 kinds of various dose behind the stomach tube with syringe respectively.First group of 5ml contains crude drug 5g; Second group of 10ml contains crude drug 10g; The 3rd group of 15ml contains crude drug 15g; The 4th group of 20ml contains crude drug 20g.
The acute filling stomach contamination of table 17 log room temperature on August 10th, 1996
Medicine: Tianjing Busuiye medicinal liquid proportion: 5.5＜extracting solution 〉
Animal varieties: large ear rabbit dosage 20ml contains crude drug 20g
| Numbering | Body weight | Dosage | Toxic action | Death time |
| ???<g> | ??20ml | Performance | 1 day 23456789 20 11 12 13 14 | |
| ??2 | ???1、6 | ???、 | ????0 | ??0??0?0?0?0?0?0?0?0?0??0??0??0??0 |
| ??15 | ???2、2 | ???、 | ????0 | ??0??0?0?0?0?0?0?0?0?0??0??0??0??0 |
| ??10 | ???2、2 | ???、 | ????0 | ??0??0?0?0?0?0?0?0?0?0??0??0??0??0 |
| ??13 | ???1、6 | ???、 | ????0 | ??0??0?0?0?0?0?0?0?0?0??0??0??0??0 |
| ??16 | ???1、6 | ???、 | ????0 | ??0??0?0?0?0?0?0?0?0?0??0??0??0??0 |
2, result:
After (1) four group of white rabbit gavages the Tianjing Busuiye medicinal liquid of 4 kinds of various dose respectively, do not have dead.None lists the existing psychological problem and the phenomena of mortality as a result after gavaging 14 days continuously, and the white rabbit weight increase is more active on the contrary.See more satin of white rabbit fur with appearance.
(2) analyze: this medicine avirulence medical material, no eighteen incompatible medicaments nineteen medicaments of mutual restraint incompatibility.
Its effect of Tianjing Busuiye medicinal liquid is: benefiting essence and marrow, help intensive culture god, YIN nourishing, tonifying YANG and heat-clearing and toxic substances removing.Therefore it is spiritual more active that White Rabbit is irritated drink more.
Five, check, probation report list
1, table 18 Tianjing Busuiye medicinal liquid hygiene inspection report
| Solution colour | Pale brown color |
| Transparency | Transparent |
| The sense of taste | Sweet, little hardship |
| PH value | ???5.5 |
| Proportion | ???1.15 |
| Through centrifugal 3000/ change/10 ' | There is not precipitation |
| 96 years JIUYUE of trier Wang Wei China 20 days | |
2, table 19 Tianjing Busuiye medicinal liquid count of bacteria report as a result
| Average bacterium colony numerical example | Test sample dilution figure place | The ratio of two dilution factor bacterium numbers | Total plate count T/ml | Total number of bacteria | ||
| ?10-1 | ???10-2 | ??10-3 | ||||
| ????1 | ????- | ??- | ＜10 | |||
| ????2 | ????- | ??- | ＜10 | |||
| ????3 | ????- | ??- | ＜10 | |||
| ????4 | ????- | ??- | ＜10 | |||
| Remarks: the equal asepsis growth of each extension rate | ||||||
Every batch of test sample extracts number: every bottle of 250ml, totally 2 bottles.
Trier: Wang Weihua.
3, table 20 Tianjing Busuiye medicinal liquid colonial morphology and probation report list
| Serum | A--F is many. valency O serum | The normal saline contrast | General biochemical reaction | Report | |
| Bacterium to be checked | Lawn | ??100° ??0.3′ | |||
| In proper order | |||||
| ????1 | ??- | ???- | ??- | Do not detect Salmonella | |
| Trier Wang Wei China JIUYUE in 1996 20 days | |||||
4, table 21 Salmonella testing procedure diagram
Test sample
↓ dilution
Test liquid
↓ cholate lactose increases bacterium and cultivated 37 ° of 37 ° ↓ 18-24 of 37 ° ↓ 18-24 hour SS and EmB flat board hour triple sugar iron agars ↓ 18-24 hour
5, Tianjing Busuiye medicinal liquid chemical constituent inspection report
(1) glycoside inspection:
Method: get test liquid 1ml, drip 2 joltings of a-naphthol reagent, drip dense stream acid, make to be divided into two liquid layers along test tube wall.
The result: purple-purplish red colour circle appears in two liquid layer intersections.(genus positive reaction)
(2) inspection of flavonoid glycoside:
Method: test liquid is directly used dissolve with ethanol (adding dissolve with ethanol if aqueous solution should concentrate) 2ml dropping concentrated hydrochloric acid, and (this moment, color reddened, illustrate and contain anthocyanidin) add then the magnesium powder granule a little, this moment in vitro promptly produce a large amount of hydrogen, bubble, warm a moment on the pond.
The result: it is red that purplish red-cherry appears in solution.(being positive reaction)
(3) inspection of Saponin:
Method: after the dilution of test liquid 1ml adding distil water, fierce jolting in test tube.
Result: produce a large amount of persistency foams and maintain more than 10 minutes more than 1/3 of foam volume, liquid volume.(being positive reaction)
(4) aminoacid, protein, polypeptide inspection:
Method: test liquid 1ml adds ninhydrin test solution number droplet, heated and boiled 4-5 minute.
Result: show redness-purple-brown-Lan Se.(being positive reaction)
6, Tianjing Busuiye medicinal liquid is to the influence (resisting fatigue experiment) of rabbit swimming time
Equipment: get that high 60cm is wide, 2 of 2 of 40cm glass jars, 20ml syringe 2 tools, stomach tube, conduit, 2 of animal fixed mounts, 1 of animal scale, 2 of gags.
Medicine: Tianjing Busuiye medicinal liquid, reference substance 0.9% normal saline.
Animal: 20 of three months little rabbit, health, male and female half and half, by same forage feed, seven day 6ml after test matched group from a mouthful administration every day: normal saline 6ml.
Method: glass jar adds water, and depth of water 50cm, water temperature remain on 20 ± 0.5 ℃, get 20 rabbits, and female, hero half and half is weighed respectively, numbered, and is divided into two groups by the body weight sex.Respectively organize rabbit fasting 4 hours before being tried, respectively rabbit is fixed on the frame, insert conduit the conduit collar extension is placed a bottle that is filled with water, prove that as gassing not this pipe is in esophagus, draw with syringe then and tried thing, inject esophagus from conduit.To Tianjing Busuiye medicinal liquid group 6ml/kg, matched group 6ml/kg normal saline, timing immediately after the injection, inject rabbit to be put in the glass jar respectively in back 30 minutes and swim, and reclocking, put into each rabbit at every turn, observe rabbit head submerged and can not the person of emerging be body weight exhaustion 10 seconds; At once timing is that swimming time and the matched group of rabbit carries out statistical procedures, and Tianjing Busuiye medicinal liquid is to the influence of three months rabbit swimming times, experimental record: table 22
| Group | Dosage ml/kg | Rabbit swimming time min |
| Normal saline | ????6ml | ???12.00±2.10 |
| Tianjing Busuiye medicinal liquid | ????6ml | ???21.96±3.42 |
According to experimental result: Tianjing Busuiye medicinal liquid has antifatigue effect.
Six, Tianjing Busuiye medicinal liquid stability preliminary study
Adopt the constant temperature accelerated test, to the research that experimentizes of the stability of Tianjing Busuiye medicinal liquid.Experimental data is learned by statistics and is handled and graphing method is asked calculation, under 25 ℃ of conditions of room temperature, with 2 years effect duration of emodin standard.
The difference of various medicines on chemically stable is very big, the compound Chinese medicinal preparation complicated component, thereby the preliminary assay of effect duration is difficult.Present stage with a certain or several active component (preferably effective ingredient), as the preparation index of effect duration, be feasible.This paper adopts the constant temperature accelerated test method, through prerun, selects 55,65,75,90 ℃ for use, to main component emodin stability prediction in the Tianjing Busuiye medicinal liquid, simultaneously the variation of pH value in the degradation process is investigated.
1, assay method: (1) sample treatment: sample is close to be filled in centrifuge tube, places 55,65,75,90 ℃ of waters bath with thermostatic control respectively, timing sampling, and cooling, standby.(2) the former state trap is measured: sample thief 40ml adds methanol 50ml, puts in the apparatus,Soxhlet's, extracts, and waves most methanol, add 2.5mol/L sulphuric acid 15ml, chloroform 15ml, hydrolysis 2 hours, be transferred to after the cooling in the separatory funnel, divide and get chloroform layer, reuse 15ml chloroform extraction once.Combined chloroform liquid, (2 * 20ml) are washed till neutrality to distilled water.Water-bath boils off chloroform, and it is a small amount of to add 0.5% magnesium acetate methanol solution, transfers to after the dissolving in the 50ml volumetric flask, is diluted to scale.With 0.5% magnesium acetate methanol reagent is blank, decides absorption value with 721 spectrophotometers in the 498nm wave length calibration.(3) instant absorption of sample degree is measured: method is with above-mentioned former state absorption measurement.(4) relative amount is asked calculation: the relative amount of each time (%)=trap * 100% when instant absorption of sample degree/former state is zero.
2, experimental result:
(1) determination data: see Table 23
(2) determine the order of reaction with diagram method,,, be shown as straight line, illustrate that its change procedure meets pseudo first-order reaction with the 1gc-t mapping according to heat time heating time and the changes of contents under each constant temperature of table 23.
(3) calculating K value: carry out linear regression according to different heating time under each constant temperature of table 23 and the logarithmic data of relative concentration, try to achieve the slope of each 1 line, and then try to achieve respective reaction rate constant K.The results are shown in Table 24.
Determination data under the different constant temperatures of table 23
| Temperature ℃ | Sample time (d) | ????0 | ????8 | ????18 | ????28 | ????38 | ????46 |
| ????55 | Measure absorption value relative concentration C (%) IgC | ??0.631 ????100 ??2.000 | ??0.614 ??97.32 ?1.9882 | ???0.590 ???93.50 ??1.9708 | ???0.569 ???90.17 ??1.9551 | ???0.550 ???87.16 ??1.9403 | ??0.539 ??85.49 ??1.9319 |
| ????65 | Measure absorption value relative concentration C (%) IgC | ??0.631 ????100 ??2.000 | ??0.602 ??95.40 ?1.9795 | ???0.583 ???92.39 ??1.9656 | ???0.561 ???88.91 ??1.9490 | ???0.510 ???80.82 ??1.9075 | ??0.466 ??73.85 ?1.8684 |
| ????75 | Measure absorption value relative concentration C (%) IgC | ??0.631 ????100 ??2.000 | ??0.582 ??92.23 ?1.9649 | ???0.540 ???85.58 ??1.9324 | ???0.486 ???77.02 ??1.8866 | ???0.412 ???65.29 ??1.8148 | ??0.392 ??62.12 ??1.7932 |
| ????90 | Measure absorption value relative concentration C (%) IgC | ??0.631 ????100 ??2.000 | ??0.525 ??83.20 ?1.9201 | ????0.42 ???66.72 ??1.8243 | ???0.341 ???54.08 ??1.7330 | ???0.268 ???42.47 ??1.6281 | ??0.229 ??36.29 ??1.5593 |
K value when (4) asking room temperature (25 ℃) is handled relevant data in the table 24, the results are shown in Table 25.With 1gk 1/T is mapped, try to achieve room temperature (1/c273+25)=3.3557 * 10
-3The time K=5.053 * 10
-4).
Measure equation of linear regression and K value under each condition of table 24
| Temperature ℃ | Regression equation | ?????r | Straight slope | The K value |
| ????55 | ???Igc＝1.9993-0.0015169t | ???0.9981 | ??-0.0015169 | ??3.4934×10 -3 |
| ????65 | ???Igc＝2.00685-0.0026892t | ???-9.9719 | ??-0.0026892 | ??6.1933×10 -3 |
| ????75 | ???Igc＝2.00548-0.0046450t | ???-0.9929 | ??-0.0046450 | ??1.0697×10 -2 |
| ????90 | ???Igc＝1.9984-0.0096028t | ???-0.9998 | ??-0.0096028 | ??2.2115×10 -2 |
Table 25 temperature and rate constant logarithm synopsis
| Temperature ℃ | ?????1/T | ???IgK |
| ????55 | ??3.0488×10 -3 | ?-2.4568 |
| ????65 | ??2.9586×10 -3 | ?-2.2081 |
| ????75 | ??2.8736×10 -3 | ?-1.9707 |
| ????90 | ??2.7548×10 -3 | ?-1.6553 |
(5) 25 ℃ of effect duration (t0.9) ask calculation, be 737 days.
(6) mensurationization of pH value in the degradation process.See Table 26 (utilizing TOAHM-20E type acidometer)
PH value changes in table 26 degradation process
| ?????55℃ | ??????65℃ | ???????75℃ | ????????90℃ | ||||
| Sample time h | PH value | Sample time h | PH value | Sample time h | PH value | Sample time h | PH value |
| ????0 | ????5.5 | ????0 | ???5.5 | ????0 | ??5.5 | ????0 | ??5.5 |
| ????4 | ????5.4 | ????6 | ???5.4 | ????3 | ??5.4 | ????2 | ??5.3 |
| ????8 | ????5.3 | ????12 | ???5.3 | ????6 | ??5.3 | ????4 | ??5.2 |
| ????12 | ????5.0 | ????18 | ???5.2 | ????10 | ??5.15 | ????6 | ??5.1 |
| ????24 | ????4.7 | ????24 | ???5.0 | ????16 | ??5.1 | ????8 | ??5.0 |
| ????48 | ????4.5 | ????36 | ???4.7 | ????24 | ??5.0 | ????12 | ??4.95 |
| ????72 | ????4.3 | ????48 | ???4.6 | ????36 | ??4.8 | ????16 | ??4.9 |
| ????144 | ????4.6 | ????72 | ???4.5 | ????48 | ??4.6 | ????24 | ??4.8 |
| ????360 | ????4.8 | ????120 | ???4.8 | ????72 | ??4.8 | ????36 | ??4.8 |
The PK value is more stable in its degradation process of Tianjing Busuiye medicinal liquid, and slight decline is arranged, and acidic materials have been described.
Conclusion
1, experiment shows after the big procatarxis temperature rise in the Tianjing Busuiye medicinal liquid downward trend is arranged, and its effect duration is 737 days.
2, pH value changes little in degradation process.
3, Tianjing Busuiye medicinal liquid is a compound preparation, and its mechanism of action is very complicated, so the compound preparation effect duration prediction of Chinese medicine is difficult.Have only the effective ingredient that utilizes medicine to carry out prerun.
Above-mentioned all kinds of test by Wusu City, Xinjiang Ruan of institute of traditional Chinese medicine can be, Dai Liman, Zhou Xudong, the Li Aiping of Yi Li state, Xinjiang Kuitun hospital finish.Clinical observation for slow down aging, treatment insufficiency of kidney-YANG is finished by Ruan Kecai, Li Yuzhi, Dai Liman, Hou Dongxia, Wang Weihua, Yang Hongcheng, Ma Yongyao, the Xu Xinwu of Wusu City, Xinjiang hospital, Zhou Xudong, Zhao Wenkun, the Li Aiping of Yi Li state, Xinjiang Kuitun hospital, the Zhu Weiping of Kuytun City hospital, Dong Zhiyi, Zou Sulan etc. participate in and finish.
Embodiments of the invention 1: auricle 25 grams, Radix Ginseng 15 grams, Hippocampus 10 grams, Herba Epimedii 30 grams, Fructus Psoraleae 35 grams, Radix Scutellariae 30 grams, Rhizoma Polygonati 30 grams, Radix Et Caulis Acanthopanacis Senticosi 30 grams on calf thymus 30 grams, calf spleen 20 grams, the pig heart; Radix Rehmanniae 30 grams, Fructus Lycii 30 grams, Radix Asparagi 25 grams, the Radix Astragali 30 grams, the Rhizoma Atractylodis Macrocephalae 25 grams, Radix Glycyrrhizae 30 grams, Radix Polygoni Multiflori 30 grams; The Radix Paeoniae Alba 30 grams, Radix Codonopsis 30 grams, Placenta Hominis 30 grams, Herba Cistanches 30 grams, Radix Isatidis 30 grams, procaine 0.05/100 milliliters of grams, Fructus Ligustri Lucidi 30 grams, Fructus Schisandrae Chinensis 30 grams.
Embodiment 2: auricle 3 grams, Radix Ginseng 3 grams, hippocampus 3 gram, Herba Epimedii 10 grams, Fructus Psoraleae 10 grams, Radix Scutellariae 10 grams, Rhizoma Polygonati 10 grams, Radix Et Caulis Acanthopanacis Senticosi 10 grams on calf thymus 8 grams, calf spleen 3 grams, the pig heart; Radix Rehmanniae 10 grams, Fructus Lycii 10 grams, Radix Asparagi 10 grams, the Radix Astragali 10 grams, the Rhizoma Atractylodis Macrocephalae 8 grams, Radix Glycyrrhizae 8 grams, Radix Polygoni Multiflori 8 grams; The Radix Paeoniae Alba 8 grams, Radix Codonopsis 8 grams, Placenta Hominis 6 grams, Herba Cistanches 8 grams, Radix Isatidis 8 grams, procaine 0.0003/100 milliliters of grams, Fructus Ligustri Lucidi 8 grams, Fructus Schisandrae Chinensis 8 grams.
Embodiment 3: auricle 15 grams, Radix Ginseng 15 grams, Hippocampus 6 grams, Herba Epimedii 30 grams, Fructus Psoraleae 25 grams, Radix Scutellariae 25 grams, Rhizoma Polygonati 25 grams, Radix Et Caulis Acanthopanacis Senticosi 25 grams on calf thymus 30 grams, calf spleen 15 grams, the pig heart; Radix Rehmanniae 15 grams, Fructus Lycii 20 grams, Radix Asparagi 15 grams, the Radix Astragali 20 grams, the Rhizoma Atractylodis Macrocephalae 15 grams, Radix Glycyrrhizae 20 grams, Radix Polygoni Multiflori 20 grams; The Radix Paeoniae Alba 15 grams, Radix Codonopsis 25 grams, Placenta Hominis 25 grams, Herba Cistanches 20 grams, Radix Isatidis 20 grams, procaine 0.0006/100 milliliters of grams, Fructus Ligustri Lucidi 20 grams, Fructus Schisandrae Chinensis 20 grams.
Embodiment 4: the manufacture method of Tianjing Busuiye medicinal liquid Chinese medicine is: selected first-class medical material clean → was pulverized 40 mesh sieves, was weighed: Radix Ginseng 3 grams, hippocampus 3 gram, Herba Epimedii 10 grams, Fructus Psoraleae 10 grams, Radix Scutellariae 10 grams, Rhizoma Polygonati 10 grams, Radix Et Caulis Acanthopanacis Senticosi 10 grams; Radix Rehmanniae 10 grams, Fructus Lycii 10 grams, Radix Asparagi 10 grams, the Radix Astragali 10 grams, the Rhizoma Atractylodis Macrocephalae 8 grams, Radix Glycyrrhizae 8 grams, Radix Polygoni Multiflori 8 grams; The Radix Paeoniae Alba 8 grams, Radix Codonopsis 8 grams, Placenta Hominis 6 grams, Herba Cistanches 8 grams, Radix Isatidis 8 grams, Fructus Ligustri Lucidi 8 grams, Fructus Schisandrae Chinensis 8 grams, merceration bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 gram amount by every milliliter and add distilled water, quiet straight 24 hours → vacuum filtration → quiet straight 24 hours → vacuum filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.
The making step of the animal drugs of joining with the foregoing description 4 medicines is: with the calf thymus of getting ready, the calf spleen, auricle is in advance at-8～-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → slurrying → take by weighing weight with distilled water on the pig heart: calf thymus 8 grams, calf spleen 3 grams, saline leaching in the life of auricle 3 gram → adding doubling doses on the pig heart, soaked 1 hour at room temperature earlier, again 75～80 ℃ soak filtered in 0.5 hour soak again 2～3 times → with the rapid heated and boiled of filtrate 3～5 minutes, making medicinal liquid slightly be chilled to 40～60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, / 100 milliliters of (0.0003～0.05%) adapteds of procaine 0.0003～0.05 gram, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.
Embodiment 5: the manufacture method of Tianjing Busuiye medicinal liquid Chinese medicine is: selected first-class medical material clean → was pulverized 40 mesh sieves, was weighed: Radix Ginseng 15 grams, Hippocampus 10 grams, Herba Epimedii 30 grams, Fructus Psoraleae 35 grams, Radix Scutellariae 30 grams, Rhizoma Polygonati 30 grams, Radix Et Caulis Acanthopanacis Senticosi 30 grams; Radix Rehmanniae 30 grams, Fructus Lycii 30 grams, Radix Asparagi 25 grams, the Radix Astragali 30 grams, the Rhizoma Atractylodis Macrocephalae 25 grams, Radix Glycyrrhizae 30 grams, Radix Polygoni Multiflori 30 grams; The Radix Paeoniae Alba 30 grams, Radix Codonopsis 30 grams, Placenta Hominis 30 grams, Herba Cistanches 30 grams, Radix Isatidis 30 grams, Fructus Ligustri Lucidi 30 grams, Fructus Schisandrae Chinensis 30 grams, merceration bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 gram amount by every milliliter and add the quiet straight 24 hours → vacuum filtration of distilled water → quiet straight 24 hours → vacuum filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.
The making step of the animal drugs of joining with the foregoing description 5 medicines is: with the calf thymus of getting ready, the calf spleen, auricle is in advance at-8～-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → slurrying → take by weighing weight with distilled water on the pig heart: calf thymus 30 grams, calf spleen 20 grams, saline leaching in the life of auricle 25 gram → adding doubling doses on the pig heart, soaked 1 hour at room temperature earlier, again 75～80 ℃ soak filtered in 0.5 hour soak again 2～3 times → with the rapid heated and boiled of filtrate 3～5 minutes, making medicinal liquid slightly be chilled to 40～60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, the Pu Lu point is because of/100 milliliters of (0.0003～0.05%) adapteds of 0.0003～0.05 gram, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.
Embodiment 6: the manufacture method of Tianjing Busuiye medicinal liquid Chinese medicine is: selected first-class medical material clean → was pulverized 40 mesh sieves, was weighed: Radix Ginseng 15 grams, Hippocampus 6 grams, Herba Epimedii 30 grams, Fructus Psoraleae 25 grams, Radix Scutellariae 25 grams, Rhizoma Polygonati 25 grams, Radix Et Caulis Acanthopanacis Senticosi 25 grams; Radix Rehmanniae 15 grams, Fructus Lycii 20 grams, Radix Asparagi 15 grams, the Radix Astragali 20 grams, the Rhizoma Atractylodis Macrocephalae 15 grams, Radix Glycyrrhizae 20 grams, Radix Polygoni Multiflori 20 grams; The Radix Paeoniae Alba 15 grams, Radix Codonopsis 25 grams, Placenta Hominis 25 grams, Herba Cistanches 20 grams, Radix Isatidis 20 grams, Fructus Ligustri Lucidi 20 grams, Fructus Schisandrae Chinensis 20 grams, merceration bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 gram amount by every milliliter and add distilled water, after quiet straight 24 hours → vacuum filtration → quiet straight 24 hours final vacuum sucking filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.
The making step of the animal drugs of joining with the foregoing description 6 medicines is: with the calf thymus of getting ready, the calf spleen, auricle is in advance at-8～-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → slurrying → take by weighing weight with distilled water on the pig heart: calf thymus 30 grams, calf spleen 20 grams, the normal saline of auricle 25 gram → adding doubling doses leaches on the pig heart, soaked 1 hour at room temperature earlier, again 75～80 ℃ soak filtered in 0.5 hour soak again 2～3 times → with the rapid heated and boiled of filtrate 3～5 minutes, making medicinal liquid slightly be chilled to 40～60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, / 100 milliliters of (0.0003～0.05%) adapteds of procaine 0.0003～0.05 gram, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.
Claims (4)
1, a kind of pharmaceutical preparation Tianjing Busuiye medicinal liquid of kidney invigorating and YANG supporting delaying senility function, it is characterized in that consisting of of Chinese powder medicine component and each constituent content: calf thymus 8～30 grams, calf spleen 3～20 grams, auricle 3～25 grams on the pig heart, Radix Ginseng 3～15 grams, hippocampus 3～10 grams, Herba Epimedii 10～30 grams, Fructus Psoraleae 10～35 grams, Radix Scutellariae 10～30 grams, Rhizoma Polygonati 10～30 grams, Radix Et Caulis Acanthopanacis Senticosi 10～30 grams; Said medicine adjuvant Radix Rehmanniae 10～30 grams, Fructus Lycii 10～30 grams, Radix Asparagi 10～25 grams, the Radix Astragali 10～30 grams, the Rhizoma Atractylodis Macrocephalae 8～25 grams, Radix Glycyrrhizae 8～30 grams, Radix Polygoni Multiflori 8～30 grams; Another ancillary drug Radix Paeoniae Alba 8～30 grams, Radix Codonopsis 8～30 grams, Placenta Hominis 6～30 grams, Herba Cistanches 8～30 grams, Radix Isatidis 8～30 grams ,/100 milliliters of procaine 0.0003～0.05 grams, Fructus Ligustri Lucidi 8～30 grams, Fructus Schisandrae Chinensis 8～30 grams.
2, Tianjing Busuiye medicinal liquid according to claim 1, it is characterized in that consisting of of Chinese powder medicine component and each component optimum content: calf thymus 30 grams, calf spleen 15 grams, auricle 15 grams on the pig heart, Radix Ginseng 15 grams, Hippocampus 6 grams, Herba Epimedii 30 grams, Fructus Psoraleae 25 grams, Radix Scutellariae 25 grams, Rhizoma Polygonati 25 grams, Radix Et Caulis Acanthopanacis Senticosi 25 grams; Ancillary drug Radix Rehmanniae 15 grams of above-mentioned principal agent, Fructus Lycii 20 grams, Radix Asparagi 15 grams, the Radix Astragali 20 grams, the Rhizoma Atractylodis Macrocephalae 15 grams, Radix Glycyrrhizae 20 grams, Radix Polygoni Multiflori 20 grams; Another group adjuvant Radix Paeoniae Alba 15 grams, Radix Codonopsis 25 grams, Placenta Hominis 25 grams, Herba Cistanches 20 grams, Radix Isatidis 20 grams ,/100 milliliters of procaine 0.0006 grams, Fructus Ligustri Lucidi 20 grams, Fructus Schisandrae Chinensis 20 grams.
3, the manufacture method of Tianjing Busuiye medicinal liquid according to claim 1 and 2, the manufacture method that it is characterized in that Tianjing Busuiye medicinal liquid Chinese medicine in turn includes the following steps: selected first-class medical material clean → was pulverized 40 mesh sieves, take by weighing weight: Radix Ginseng 3～15 grams, hippocampus 3～10 grams, Herba Epimedii 10～30 grams, Fructus Psoraleae 10～35 grams, Radix Scutellariae 10～30 grams, Rhizoma Polygonati 10～30 grams, Radix Et Caulis Acanthopanacis Senticosi 10～30 grams; Radix Rehmanniae 10～30 grams, Fructus Lycii 10～30 grams, Radix Asparagi 10～25 grams, the Radix Astragali 10～30 grams, the Rhizoma Atractylodis Macrocephalae 8～25 grams, Radix Glycyrrhizae 8～30 grams, Radix Polygoni Multiflori 8～30 grams; The Radix Paeoniae Alba 8～30 grams, Radix Codonopsis 8～30 grams, Placenta Hominis 6～30 grams, Herba Cistanches 8～30 grams, Radix Isatidis 8～30 grams, Fructus Ligustri Lucidi 8～30 grams, Fructus Schisandrae Chinensis 8～30 gram mercerations bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 gram amount by every milliliter and add distilled water after quiet straight 24 hours → vacuum filtration → quiet straight 24 hours final vacuum sucking filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.
4, the manufacture method of Tianjing Busuiye medicinal liquid according to claim 1 and 2, the animal drugs extracting method that it is characterized in that Tianjing Busuiye medicinal liquid may further comprise the steps successively: with the calf thymus of getting ready, the calf spleen, auricle is in advance at-8～-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → slurrying → take by weighing weight calf thymus 8～30 grams with distilled water on the pig heart, calf spleen 3～20 grams, the normal saline of auricle 3～25 gram → adding doubling doses leaches on the pig heart, earlier room temperature soak 1 hour again 75～80 ℃ soak filtered in 0.5 hour soak again 2～3 times → with the rapid heated and boiled of filtrate 3～5 minutes, making medicinal liquid slightly be chilled to 40～60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively can be with Chinese medicine, animal drugs, / 100 milliliters of adapteds of procaine 0.0003～0.05 gram, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.
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| CN1318068C (en) * | 2002-12-19 | 2007-05-30 | 张盛 | Tonic liquid and preparing process thereof |
| CN100382828C (en) * | 2004-12-22 | 2008-04-23 | 那顺布赫 | Medication of prolonging life for dispelling senile plaque, and preparation method |
| CN100428897C (en) * | 2002-08-16 | 2008-10-29 | 浙江寿峰堂生物工程有限公司 | Health care food for antifatigue and improving subhealthy and its preparation method |
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| CN1089148A (en) * | 1993-01-03 | 1994-07-13 | 西安中兴保健科学研制所 | A kind of Yin-nourishing and Yang-strengthening health Chinese medicine |
| CN1027622C (en) * | 1993-02-26 | 1995-02-15 | 张益民 | A tonic and health protecting preparation |
| CN1104912A (en) * | 1994-01-07 | 1995-07-12 | 周荣华 | Oral liquid for invigorating kidney and strengthening yang and its production method |
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| CN100428897C (en) * | 2002-08-16 | 2008-10-29 | 浙江寿峰堂生物工程有限公司 | Health care food for antifatigue and improving subhealthy and its preparation method |
| CN1318068C (en) * | 2002-12-19 | 2007-05-30 | 张盛 | Tonic liquid and preparing process thereof |
| CN100382828C (en) * | 2004-12-22 | 2008-04-23 | 那顺布赫 | Medication of prolonging life for dispelling senile plaque, and preparation method |
| CN101024015B (en) * | 2006-02-19 | 2011-12-07 | 徐凌 | Caroprocaprae Gutturosae, ginseng, lycium extract for treating scapulohumeral periarthritis, arthromyodynia, impotence, prospermia and sub-health |
| JP2009269902A (en) * | 2008-05-08 | 2009-11-19 | Ind Technol Res Inst | Chinese herbal medicine extract introducing production of interferon by immunocyte and activating toll-like receptor |
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