CN1887101A - Slimming tea and its prepn - Google Patents
Slimming tea and its prepn Download PDFInfo
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- CN1887101A CN1887101A CN 200610083791 CN200610083791A CN1887101A CN 1887101 A CN1887101 A CN 1887101A CN 200610083791 CN200610083791 CN 200610083791 CN 200610083791 A CN200610083791 A CN 200610083791A CN 1887101 A CN1887101 A CN 1887101A
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Abstract
The present invention discloses one kind of slimming health tea and its preparation process. The slimming health tea consists of green tea 5.9-6.1 weight portions, honeysuckle 3.9-4.1 weight portions, cassia seed 2.9-3.1 weight portions, lotus leaf 2.9-3.1 weight portions, gynostemma pentaphylla 1.4-1.6 weight portions, haw 1.4-1.6 weight portions, and honey 5.9-6.1 weight portions. The preparation process includes the steps of compounding material, rinsing, crushing, extracting, preparing mixture particle, packing, sterilizing, etc. The slimming health tea possesses slimming function and no toxic side effect.
Description
Technical field
The present invention relates to health food, relate in particular to a kind of health protection tea and preparation method thereof with weight losing function.
Background technology
Along with the quickening of modern life rhythm, the change of life style, simple obesity becomes in the life very general phenomenon, and obesity can cause numerous disease, and for example the disease of hypertension, high fat of blood and cardiovascular aspect is all relevant with obesity.Traditional weight reduction with drugs method all adopts the method for appetite-suppressing or diarrhoea, and these slimming medicines are edible for a long time can to produce great side effect to human body.
Chinese herbal medicine is the traditional medicine of China, and it is little to have toxicity, safe advantage.Utilize various refinement processing with plant of efficacy of drugs through certain technology, and by the Chinese herbal medicine slim tea that specific component is prepared, the effect that not only can play fat-reducing after eating is the technical problem that people make great efforts to solve by not producing other side effects to human body always.Tea can quench one's thirst and refresh oneself, its function of slaking thirst and help produce saliva is that present any beverage can't be compared, tea contains more than 500 kind of necessary for human body abundant nutrition composition, what wherein have trophism has protein, amino acid, polysaccharide, vitamin and inorganic salts, and also contain abundant bioactivator-Tea Polyphenols in the tealeaves, make tea also have the effect of anti-ageing, anti-cancer and cancer-preventing and fat-reducing.Honeysuckle cassia seed lotus leaf gynostemma pentaphylla hawthorn honey.Also there is not at present a kind of health protection tea that utilizes above-mentioned batching to be mixed with simply to brew with antiobesity action.
Summary of the invention
In view of this, one of main purpose of the present invention is to provide a kind of health protection tea with weight losing function
For achieving the above object, health protection tea with weight losing function of the present invention is made of by weight following ingredients: green tea: 5.9-6.1 part, honeysuckle: 3.9-4.1 part, cassia seed: 2.9-3.1 part, lotus leaf: 2.9-3.1 part, gynostemma pentaphylla 1.4-1.6 part, hawthorn: 1.4-1.6 part, honey: 5.9-6.1 part.
Further, described green tea is selected from green tea more than three grades;
Further, described slim tea by following ingredients by weight forming: green tea: 6 parts, honeysuckle: 4 parts, cassia seed: 3 parts, lotus leaf: 3 parts, 1.5 parts of gynostemma pentaphylla, hawthorn: 1.5 parts, honey: 6 parts.
Another object of the present invention is to provide a kind of method for preparing above-mentioned health protection tea, this method may further comprise the steps:
A prepares burden by prescription, checked the mark in green tea, honeysuckle, cassia seed, lotus leaf, gynostemma pentaphylla, mountain and do not rinse well repeatedly with flowing water, and after oven dry under the 70-90 ℃ of condition, it is standby to pulverize 20 mesh sieves more respectively;
Gynostemma pentaphylla after b will clean, pulverize, hawthorn are put into extractor respectively and extract 2 times; For the first time add 10 times of water, soaked 1.5-2.5 hour, little boiling decocted 1.5-2.5 hour, filtered extract, continued to employ; Add for the second time 6 times of water, little boiling decocted 1.5-2.5 hour, filtered extract, merged extracted twice liquid; The extract vacuum being concentrated into density again is about 1.0-1.2, and the gained concentrate is carried out spray-drying, promptly obtains gynostemma pentaphylla, hawthorn powder respectively, standby;
C mixes the powder of green tea, honeysuckle, cassia seed, lotus leaf with step b gained gynostemma pentaphylla, hawthorn powder powder, evenly stirred 4 hours in agitator, gets mixture of powders;
In the mixture that d is obtained, and add water even stirring 3.5-4.5 hour in agitator that weight is honey amount 1/4 in step c; Get particulate mixtures;
E crosses 5 mesh sieves with steps d gained particulate mixtures, gets hybrid fine particles;
F is dried hybrid fine particles 1-2 hour under 80 ℃ of conditions, and moisture is reduced to below 4%;
Hybrid fine particles after g will be dried is distributed into bag on demand, adopts radiosterilization then, makes qualified health protection tea.
Further, the bake out temperature among the described step a is 80 ℃, and soak time described in the step b is 2 hours, and little decocting time that boils is 2 hours for the first time, and little decocting time that boils is 2 hours for the second time, and it is 1.1 that vacuum concentrates density, and the mixing time of steps d is 4 hours;
Further, described steps d-g carries out in the health cleanliness factor is 100,000 grades dust proof workshop;
Further, the disinfection way of described step g is 7.5KGYCo
60Radiosterilization.
Health protection tea of the present invention after the people drinks, does not have any harmful effect, and proves through functional trial owing to adopt natural plant and unique prescription, and long-term drinking has tangible weight losing function, drinks conveniently; And preparation technology of the present invention is simple, is suitable for industrialization production.
Other advantages of the present invention will be set forth to a certain extent in the following description, and to a certain extent, based on being conspicuous to those skilled in the art, perhaps can obtain instruction from the practice of the present invention to investigating hereinafter.Target of the present invention and other advantages can be passed through following specification, claims, and the specifically noted structure realizes and obtains in the accompanying drawing.
Description of drawings
In order to make the purpose, technical solutions and advantages of the present invention clearer, the present invention is described in further detail below in conjunction with accompanying drawing.
Accompanying drawing is technological process of production figure of the present invention.
The specific embodiment
Hereinafter with reference to accompanying drawing, the preferred embodiments of the present invention are described in detail.
Embodiment 1
Get green tea 590mg, honeysuckle: 390mg, cassia seed: 290mg, lotus leaf: 290mg, gynostemma pentaphylla 140mg, hawthorn: 140mg, honey: 590mg.
What a will prepare rinses green tea, honeysuckle, cassia seed, lotus leaf, gynostemma pentaphylla, hawthorn well with flowing water respectively repeatedly, and after oven dry under 70 ℃ of conditions, it is standby to pulverize 20 mesh sieves more respectively;
Gynostemma pentaphylla after b will clean, pulverize, hawthorn are put into extractor respectively and extract 2 times; Add for the first time 10 times of water, soak 1.5, hour, little boil decoct 1.5, hour, filter extract, continue to employ; Add for the second time 6 times of water, little boil decoct 1.5, hour, filter extract, merge extracted twice liquid; The extract vacuum being concentrated into density again is 1.0, and the gained concentrate is carried out spray-drying, promptly obtains gynostemma pentaphylla, hawthorn powder respectively, standby;
C mixes the powder of green tea, honeysuckle, cassia seed, lotus leaf with step b gained gynostemma pentaphylla, hawthorn powder powder, evenly stirred 4 hours in agitator, gets mixture of powders;
In the mixture that d is obtained, and add water even stirring 3.5 hours in agitator that weight is honey amount 1/4 in step c; Get particulate mixtures;
E crosses 5 mesh sieves with steps d gained particulate mixtures, gets hybrid fine particles;
F is dried hybrid fine particles 1-2 hour under 80 ℃ of conditions, and moisture is reduced to below 4%;
Hybrid fine particles after g will be dried is distributed into bag on demand, adopts 7.5KGYCo then
60Radiosterilization makes qualified health protection tea.
Described steps d-g carries out in the health cleanliness factor is 100,000 grades dust proof workshop.
Embodiment 2
Get green tea 600mg, honeysuckle: 400mg, cassia seed: 300mg, lotus leaf: 30mg, gynostemma pentaphylla 150mg, hawthorn: 150mg, honey: 600mg.
What a will prepare rinses green tea, honeysuckle, cassia seed, lotus leaf, gynostemma pentaphylla, hawthorn well with flowing water respectively repeatedly, and after oven dry under 70 ℃ of conditions, it is standby to pulverize 20 mesh sieves more respectively;
Gynostemma pentaphylla after b will clean, pulverize, hawthorn are put into extractor respectively and extract 2 times; For the first time add 10 times of water, soaked 2 hours, little boiling decocted 2 hours, filtered extract, continued to employ; Add for the second time 6 times of water, little boil decoct 1.5, hour, filter extract, merge extracted twice liquid; The extract vacuum being concentrated into density again is 1.1, and the gained concentrate is carried out spray-drying, promptly obtains gynostemma pentaphylla, hawthorn powder respectively, standby;
C mixes the powder of green tea, honeysuckle, cassia seed, lotus leaf with step b gained gynostemma pentaphylla, hawthorn powder powder, evenly stirred 4 hours in agitator, gets mixture of powders;
In the mixture that d is obtained, and add water even stirring 4 hours in agitator that weight is honey amount 1/4 in step c; Get particulate mixtures;
E crosses 5 mesh sieves with steps d gained particulate mixtures, gets hybrid fine particles;
F is dried hybrid fine particles 1-2 hour under 80 ℃ of conditions, and moisture is reduced to below 4%;
Hybrid fine particles after g will be dried is distributed into bag on demand, adopts 7.5KGYCo then
60Radiosterilization makes qualified health protection tea.
Described steps d-g carries out in the health cleanliness factor is 100,000 grades dust proof workshop.
Embodiment 3
Get green tea 610mg, honeysuckle: 410mg, cassia seed: 310mg, lotus leaf: 310mg, gynostemma pentaphylla 160mg, hawthorn: 160mg, honey: 610mg.
What a will prepare rinses green tea, honeysuckle, cassia seed, lotus leaf, gynostemma pentaphylla, hawthorn well with flowing water respectively repeatedly, and after oven dry under 90 ℃ of conditions, it is standby to pulverize 20 mesh sieves more respectively;
Gynostemma pentaphylla after b will clean, pulverize, hawthorn are put into extractor respectively and extract 2 times; Add for the first time 10 times of water, soak 2.5, hour, little boil decoct 2.5, hour, filter extract, continue to employ; Add for the second time 6 times of water, little boil decoct 2.5, hour, filter extract, merge extracted twice liquid; The extract vacuum being concentrated into density again is 1.2, and the gained concentrate is carried out spray-drying, promptly obtains gynostemma pentaphylla, hawthorn powder respectively, standby;
C mixes the powder of green tea, honeysuckle, cassia seed, lotus leaf with step b gained gynostemma pentaphylla, hawthorn powder powder, evenly stirred 4 hours in agitator, gets mixture of powders;
In the mixture that d is obtained, and add water even stirring 4.5 hours in agitator that weight is honey amount 1/4 in step c; Get particulate mixtures;
E crosses 5 mesh sieves with steps d gained particulate mixtures, gets hybrid fine particles;
F is dried hybrid fine particles 1-2 hour under 80 ℃ of conditions, and moisture is reduced to below 4%;
Hybrid fine particles after g will be dried is distributed into bag on demand, adopts 7.5KGYCo then
60Radiosterilization makes qualified health protection tea.
Described steps d-g carries out in the health cleanliness factor is 100,000 grades dust proof workshop.
Using method: adopting the every pouch weight of slim tea of above-mentioned prescription and preparation method preparation is 2500mg, and the 1-2 bag pouch of being grown up is put into cup every day during use, brews with boiling water and drinks while hot after 5-8 minute, can brew repeatedly, and the crowd that is not suitable for is juvenile and pregnant woman.
Experimental result:
Safety testing
Sample: by the foregoing description method preparation have an effect of weight reducing tea in bag, content is the yellowish-brown particle.Shady and cool dry place, sealing is preserved.The oral recommended amounts of human body is 0.167g/kgbw.Get the 835g sample and soaked 5-10 minute with boiling water to specifications, repeat to soak 2 times, merging 2 soaks, to be concentrated into 1000ml standby.
Animal used as test and environment: cleaning level SD rat, Kunming mouse.21 ℃-25 ℃ of experimental session experimental situation temperature, humidity 40%-58%.
Test unit: disease prevention and control center, Hunan Province
Acute toxicity test in mice: selecting body weight for use is 20 of the healthy Kunming mouses of 18g-22g, male and female half and half.Sample thief is pressed 21500ml/kgbw dosage, at interval 3 hours at twice per os irritate stomach, irritate at every turn that body of stomach is long-pending to be 0.2ml/10gbw.Observed for two weeks continuously after irritating stomach, record poisoning manifestations and death condition, result of the test sees Table 1
Salmonella reversion test: employing is tested through identifying satisfactory salmonella typhimurium histidine defect type TA97, TA98, TA100, TA102 four strain bacterial strains.Adopt rat liver microsomes enzyme (S-9) that Polychlorinated biphenyls (PCB) induces as external metabolism activation system.Adding 0.1ml test strain enrichment liquid, 0.1ml are tried thing solution and 0.5ml S-9 mixed liquor (when need wife metabolism activation) in top agar, pour into behind the mixing on the bottom culture medium flat plate, five test doses are respectively 5000,1000,200,40,8vdnn, and sample thief is assigned to desired concn with distilled water.Establish simultaneously from beaming back change, solvent control and the contrast of positive mutagens.Cultivated 48 hours, and counted every ware clump count.Become more than 2 times of clump count if try the increase of returning the change clump count of thing above beaming back certainly, and have dosage one reaction relation person to be decided to be the positive.A whole set of test repeats to do twice under same test conditions.Result of the test sees Table 2-3
PCEMNR micronucleus test: adopt the 24 hours twice per os administration by gavage in interval to test.Get body weight and be 60 of the healthy Kunming mouses of 25g-30g, be divided into 6 groups at random, 10 every group, male and female half and half.With the positive contrast of the endoxan of 40mg/kgbw dosage, the negative contrast of distilled water.4 dosage of test group are respectively 10000,5000,2500,1250mg/kgbw, get the slim tea soak and are assigned to desired concn with distilled water.Animal was put to death in cervical vertebra dislocation in 6 hours after last was given sample, got bone marrow of sternum and diluted smear with calf serum, and methyl alcohol is fixed, Giemsa dyeing.Under the light microscope, every animal counting 1000 polychromatic erythrocytes (PCE), microkernel incidence is pressed the Poisson distribution statistics and is handled to contain the PCE permillage of micronucleus.Result of the test sees Table 4
Mouse is skillful in deformity test: get 25 of the male mice in kunming of body weight 25g-35g, be divided into 5 groups at random, 5 every group.With the positive contrast of the endoxan of 40mg/kgbw dosage, the negative contrast of distilled water.The high, medium and low dosage of test group is respectively 10000,5000,2500mg/kgbw, gets above-mentioned slim tea soak and is assigned to desired concn with distilled water.Irritate stomach every day once, continuous 5 days, put to death animal in the 30th day after last is irritated stomach, get the epididymis smear, Yihong dyeing, every animal counting, being skillful in of 000 structural integrity calculated distortion and is skillful in incidence.Result of the test sees Table 5
30 days feeding trials: select the SD rat of ablactation just to be divided into four groups at random, promptly control group and three are tried the chimney group, and 20 every group, male and female half and half.Basic, normal, high three are tried thing group dosage and are respectively 4.18g/kg.bw, 8.35g/kg.bw, 16.70g/kg.bw, are equivalent to 25,50 of human body RD approximately; 100 times, get soak and call distilled water together and be assigned to desired concn.Control group gives isopyknic distilled water.Give animal subject respectively and irritate stomach, irritate stomach every day once, irritate the long-pending 2ml/100g.bw of being of body of stomach, continuous 30 days.Each treated animal is all fed with normal diet, feeds free diet, drinking-water in the cage.Observe activity and the growing state of animal every day, record forward and back appetite claims body weight weekly one time, calculates food utilization.Experiment end is adopted tail point blood and is measured hemoglobin (Hb), packed cell volume (HCT) with the full-automatic blood cell gauge of FC-1100, carry out red blood cell (RBC), leucocyte (WBC)) I. platelet (PLT) counting and WBC classification.After the fasting 14 hours, pluck eyeball blood sampling separation of serum, measure total protein (TP), albumin (ALB), creatinine (Cr), urea nitrogen (BUN), glutamic-pyruvic transaminase (ALT), glutamic-oxalacetic transaminease (AST), cholesterol (CHOL), triglyceride (TG) and blood sugar (GLU) with Kang Ren 560 huge automatic biochemistry analyzers, the cervical vertebra dislocation is put to death animal and is done gross anatomy, claim liver,kidney,spleen, testis, ovary weight, calculate dirty/body ratio, get liver, kidney, stomach, spleen, duodenum, testis, ovary and do the pathology cut sections for microscopic examination.Result of the test sees Table 6-18
From table 1-18 as can be seen, above-mentioned slim tea soak all greater than 21500mg/kg.bw, belongs to non-poisonous material to the acute oral LD50 of female, male mouse.The result is all negative for three genetic toxicity tests (Salmonella reversion test, PCEMNR micronucleus test, mouse are skillful in the deformity test).With 4.18,8.35,16.70kg.bw the slim tea soak irritated stomach 30 days to rat oral, duration of test, each treated animal grows well, each dosage group body weight, weightening finish, food utilization, routine blood test index (hemoglobin, the red blood cell sum, packed cell volume, total number of blood platelet, total white blood cells and classification), blood biochemistry index (serum glutamic pyruvic transminase, glutamic-oxalacetic transaminease, total protein, albumin, cholesterol, triglycerides, urea nitrogen, creatinine, blood sugar), organ coefficient (liver/body, spleen/body, the testis of kidney/body ratio and male mouse/body weight ratio) compares there was no significant difference (P＞0.05) with control group.Gross anatomy is not seen the abnormal change relevant with sample with tissue pathology checking, points out to feed in 30 days the every observation index of rat is not produced obvious toxic-side effects.
Assay:
According to result of the test, above-mentioned slim tea soak toxicological assessment result:
1. acute oral LD
50Result:,, belong to non-poisonous material all greater than 21500mg/kg.bw to acute oral LD50 female, male mouse.
2. three genetic toxicity test results: the result is all negative for three genetic toxicity tests (Salmonella reversion test, PCEMNR micronucleus test, mouse are skillful in the deformity test);
3.30 it feeding trial result: with 4.18,8.35, the slim tea soak of 16.70g/kg.bw irritated stomach 30 days to rat oral, duration of test, animal growth is good, each dosage group body weight, weightening finish, food utilization, routine blood test index, blood biochemistry index, organ coefficient and control group compare, there was no significant difference (P＞0.05).Gross anatomy is not seen the abnormal change relevant with sample with tissue pathology checking.The prompting submitted sample was fed the every observation index of rat is not produced obvious toxic-side effects in 30 days.The report of slim tea effect human feeding trial
Sample:
Press the bag bubble slim tea of the foregoing description preparation, content is the yellowish-brown particle.
Study subject:
Simple obesity person through health check-up, does not have dysfunctions such as the obvious heart, liver, courage, kidney.
Experimenter's choice criteria:
20% of the body weight that is above standard.SBW computing formula: standard body gross ton two (height cm-100) * 0.9; Overweight degree %=[(body weight ton-SBW k8)/standard body important place 1 *] 00%.
Exclusion standard:
Merge to have the inclination, serious former drawing property diseases such as liver, kidney and hemopoietic system, the mental patient; Eat in accordance with regulations and tried thing, can't judge effect or data not umbra sound effect or security judgement person.
The test-meal method:
32 routine simple obesity experimenters take slim tea every day 2 times, and each 2 bags, put into boiling water (about 500mi), soak and drink after 5-10 minute, in drinking continuous 30 days after meal half an hour.
Key instrument: sebum thickness meter, bioelectrical impedance analysis instrument (OMRON-HBF-306), cycle ergometer
Observation index: lift and examine 30 days, every index begins and finishes each test once in the test-meal experiment.
Ordinary circumstance:
Sleep, spirit, stool and urine.
Security is observed:
Blood routine is checked: red blood cell count(RBC), hemoglobin
Routine urinalysis (comprising the urine ketoboidies) and stool routine examination:
Biochemical indicator is measured: albumin (ALB), total protein (TP), glutamic-oxalacetic transaminease (AS fourth), glutamic-pyruvic transaminase (ALT), urea (UREA), creatinine (CRE), blood uric acid (UA), blood sugar (GLU), triglycerides (TG), T-CHOL (CHOL).Abdominal B type ultrasonography, electrocardiogram, the fluoroscopy of chest of X line.Body weight and height: emptying stool and urine before measuring, to take off one's shoes, the test-meal forebody-afterbody is resurveyed and is regularly all worn same clothes and measure in same doctor's type scale.
Bioelectrical impedance analysis: measure the human body body fat rate.
Measurements of the chest, waist and hips are measured:
Waistline: the abdomen girth of flat navel;
Hip circumference: with the girth of both sides greater trochanter of femur level.
The sebum thickness measurement:
Measure the position: right deltoid muscle lower edge arm outside mid-point;
Right angulus inferior scapulae;
The other 3cm of right navel;
Right anterior superior spine.
The maximal oxygen uptake test: the experimenter uses cycle ergometer to measure exercise tolerance (50 watts of power, 5 minutes time) before and after fat-reducing.The record exercise heart rate is inferred experimenter's maximal oxygen uptake.
Experimental data statistics: carry out statistical analysis with INSTA software.
The result:
Ordinary circumstance: after the test-meal test, experimenter's mental status, sleep quality, stool and urine situation no abnormality seen.
The security observation index detects: before and after the test-meal, it is all negative that the experimenter urinates the ketoboidies result.
Blood routine and biochemical indicator are measured: see Table 19 every indexs before and after test-meal all in normal range (NR).
Abdominal B type ultrasonography, electrocardiogram, the fluoroscopy of chest of X line detect: all in normal range (NR).
Conclusion: the edible continuously slim tea of experimenter is after 30 days, and body weight, body fat total amount, body fat percentage all descend, weight average decline 3.1kg, and the body fat gross weight 2.4kg that on average descends, body fat percentage on average descends 1.8%; Subcutaneous fat thickness all descends, and wherein the deltoid muscle place reduces 4.8mm, and omoplate reduces 4.6mm in the place down, and the other place of navel reduces 7.1111m, and ilium sour jujube place reduces 4.8mm; Waistline reduces 4.5cm, and hip circumference reduces 2.3cm, with comparing difference before the test conspicuousness (P＜0.05 or P＜0.001) is arranged all.All in range of normal value, exercise tolerance does not fall the every clinical indices of experimenter as follows before and after the test.This shows that above-mentioned slim tea has antiobesity action to human body, and body health is not had obvious damage.
Table 1 slim tea is to the acute toxicity of mouse
| Animal varieties | Sex | Animal (only) | Approach | Dosage (mg/kgbw) | Death toll (only) | LD 50(mg/kg·bw) |
| The mouse mouse | Male and female | 10 10 | The per os per os | 21500 21500 | 0 0 | ＞21500 ＞21500 |
Table 2 slim tea Salmonella reversion test result (for the first time) (individual/ware)
| Tried thing | Dosage (ug/ ware) | TA97 | TA98 | TA100 | TA102 | ||||
| +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | ||
| Tried thing | 5000 | 143.7+7.4 | 153.3+4.6 | 33.3+1.5 | 37.3+2.1 | 178.3+5.5 | 164.3+6.1 | 291.3+10.5 | 275.7+10.5 |
| l000 | 156.0±5.0 | 143.7±7.5 | 33.0±2.6 | 38.3±1.5 | 164.0±7.2 | 177.3±3.2 | 277.7±7.0 | 298.0±5.6 | |
| 200 | 159.3±5.1 | 145.3±9.5 | 38.7±1.5 | 33.3±1.5 | 175.0±6.6 | 164.7±7.4 | 276.7±5.0 | 297.7±5.1 | |
| 40 | 145.7±4.7 | 155.7±9.1 | 34.3±2.1 | 39.7±2.1 | 162.7±5.0 | 176.3±7.2 | 297.3±10.8 | 276.0±8.0 | |
| 8 | 156.3±5.5 | 148.0±2.0 | 40.0±2.6 | 34.3±2.1 | 173.7±8.1 | 160.7±8.1 | 275.3±5.0 | 296.7±5.7 | |
| From beaming back change | 157.7±8.5 | 144.3±7.1 | 33.0±1.0 | 40.0±4.6 | 160.7±4.0 | 174.0±7.2 | 297.0±7.8 | 279.7±13.1 | |
| Solvent control | 144.3±9.7 | 158.0±9.2 | 39.7±3.1 | 33.7±2.1 | 163.7±7.8 | 176.7±8.4 | 274.3±12.0 | 299.7±7.8 | |
| The 9-Fluorenone | 0.2 | 1239.3±66.3 | 2248.3±47.5 | ||||||
| NaN 1 | 2.5 | 2294.7±53.1 | |||||||
| 1.8-istizin | 50.0 | 1054.3±68.1 | |||||||
| MMC | 4.0 | 2057.5±83.7 | |||||||
| 2-AF | 10.0 | 1267.3±45.9 | 3169.3±44.5 | 2147.3±75.9 | |||||
Annotate: above result is the means standard deviation of 3 plates
Table 3 slim tea Salmonella reversion test result (for the second time) (individual/ware)
| Tried thing | Dosage (ug/ ware) | TA97 | TA98 | TA100 | TA102 | ||||
| +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | +S9 | -S9 | ||
| Tried thing | 5000 | 155.3+11.2 | 145.7+10.4 | 34.0+2.0 | 41.7+3.5 | 161.0+7.8 | 176.0+7.0 | 279.0+15.6 | 296.0+9.5 |
| 1000 | 144.7±3.2 | 158.0±6.0 | 38.3±4.9 | 34.3±2.5 | 174.7±8.1 | 164.7±2.5 | 297.0±9.8 | 284.3±11.4 | |
| 200 | 155.0±10.8 | 147.0±7.9 | 34.3±1.5 | 38.7±2.5 | 160.3±9.7 | 174.3±6.8 | 281.7±7.0 | 295.7±12.0 | |
| 40 | 143.0±3.6 | 155.3±5.9 | 38.0±2.6 | 34.7±1.5 | 176.7±7.8 | 163.0±4.6 | 298.7±10.0 | 282.0±10.4 | |
| 8 | 147.0±7.8 | 155.7±7.5 | 41.0±3.0 | 34.0±2.6 | 161.3±7.2 | 177.0±7.9 | 281.7±6.4 | 298.0±9.6 | |
| From beaming back change | 155.3±7.5 | 144.7±7.1 | 34.7±1.5 | 40.7±4.0 | 176.0±8.9 | 163.0±5.6 | 296.0±12.2 | 283.7±8.5 | |
| Solvent control | 146.3±7.4 | 155.3±7.5 | 41.0±2.6 | 33.7±2.1 | 163.3±6.1 | 176.7±8.1 | 279.7±7.2 | 299.3±7.4 | |
| The 9-Fluorenone | 0.2 | 1217.3±33.6 | 2254.3±95.1 | ||||||
| NaN 1 | 2.5 | 2320.0±96.7 | |||||||
| 1.8-istizin | 50.0 | 1038.0±45.0 | |||||||
| MMC | 4.0 | 2169.3±29.4 | |||||||
| 2-AF | 10.0 | 1252.7±43.1 | 3125.0±97.5 | 2224.0±70.5 | |||||
Annotate: above result is the means standard deviation of 3 plates
Table 4 slim tea is to the influence of mouse Bone marrow cells micronucleus incidence
| Sex | Dosage (mg/kgbw) | Number of animals (only) | Check cell number (individual) | Micronucleus number (individual) | Micronuclear rates (‰) |
| Male | 10000 5000 2500 1250 0 40(cp) | 5 5 5 5 5 5 | 5000 5000 5000 5000 5000 5000 | 8 8 8 7 8 124 | 1.6 1.6 1.6 1.4 1.6 24.8 |
| Female | 10000 5000 2500 1250 0 40(cp) | 5 5 5 5 5 5 | 5000 5000 5000 5000 5000 5000 | 8 8 7 8 8 124 | 1.6 1.6 1.4 1.6 1.6 22.4 |
Table 5 slim tea is to the mouse sperm deformity result of the test
| Dosage (mg/kg.bw) | Number of animals (only) | Examined sperm count (individual) | Teratospermia number (individual) | Rate of teratosperm (%) |
| 10000 5000 2500 0 40(cp) | 5 5 5 5 5 | 5000 5000 5000 5000 5000 | 98 99 106 95 603 | 1.96 1.98 2.12 1.90 12.06 |
Table 6 slim tea is to the influence of rat body weight (X ± S)
| Sex | Group | Body weight (g) | ||||
| Before the experiment | The 1st week | The 2nd week | The 3rd week | The 4th week | ||
| Male | Dosage group high dose group in the control group low dose group | 91.50±6.10 91.70±7.52 91.30±7.94 91.60±7.14 | 134.74±9.34 132.40±8.25 130.69±6.56 131.59±7.24 | 173.77±11.20 171.02±7.90 170.70±8.30 171.00±12.20 | 209.66±13.20 206.74±12.40 203.92±14.80 206.00±13.90 | 248.89±15.00 245.54±13.30 242.13±20.20 241.39±20.70 |
| Female | Dosage group high dose group in the control group low dose group | 85.60±7.26 85.50±6.33 85.60±4.06 85.50±5.88 | 119.42±9.18 117.00±9.97 115.85±8.57 117.35±10.08 | 153.18±9.50 152.21±13.50 151.72±10.10 152.70±11.80 | 181.14±11.60 181.70±14.10 181.18±12.30 182.88±12.80 | 201.57±11.30 199.21±14.80 197.56±20.30 195.41±17.20 |
Table 7 slim tea is to the influence of rat food utilization (x ± s)
| Sex | Group | Weight gain (g) | Food-intake (g) | Food utilization (%) |
| Male | Dosage group high dose group in the control group low dose group | 157.40±14.50 154.21±8.80 150.85±14.70 149.80±14.80 | 535.80±20.30 523.10±24.60 531.50±25.20 522.30±32.60 | 29.40±3.20 29.60±2.50 28.40±2.40 28.80±3.10 |
| Female | Dosage group high dose group in the control group low dose group | 115.90±7.00 113.70±10.30 111.97±19.00 110.00±13.20 | 494.20±21.10 483.20±17.70 490.80±23.20 477.20±25.50 | 23.50±1.50 23.60±2.40 22.80±3.30 23.10±3.10 |
30 days feeding trial blood tests of table 8 slim tea result (x ± s)
| Sex | Group | Number of animals (only) | Hemoglobin (g/L) | Red blood cell sum (* 10 12/L) | Packed cell volume (L/L) | Total number of blood platelet (* 10 9/L) |
| Male | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 167.40±19.27 171.90±19.33 163.20±20.98 171.90±12.68 | 6.63±0.72 7.11±0.89 6.54±1.02 6.78±0.51 | 0.467±0.054 0.489±0.066 0.455±0.070 0.493±0.037 | 299.3±45.8 284.2±37.2 294 8±62.5 268.6±46.2 |
| Female | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 175.40±11.63 179.40±18.40 179.90±8.67 170.50±10.38 | 6.93±0.74 6.99±0.80 6.85±0.39 6.91±0.37 | 0.487±0.043 0.502±0.056 0.504±0.022 0.502±0.034 | 270.9±45.9 272.0±42.4 292.8±65.9 258.8±37.7 |
Table 9 slim tea is to the influence of rat WBC (x ± s)
| Sex | Group | Number of animals (only) | Total white blood cells (* 10 9/L) | Leukocyte differential count (%) | ||
| Lymph | Other | Neutral | ||||
| Male | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 20.84±4.96 20.35±2.97 20.08±5.77 21.71±5.48 | 65.10±6.63 64.89±4.83 65.23±4.73 65.37±4.13 | 6.40±3.52 6.37±2.78 4.58±2.88 6.45±3.19 | 28.53±7.04 28.74±4.88 30.18±4.04 28.18±5.96 |
| Female | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 20.59±5.91 21.32±4.70 23.77±4.56 20.29±2.87 | 64.34±7.62 65.34±6.27 68.90±7.78 69.33±7.58 | 5.30±4.74 5.51±2.00 5.92±2.36 7.73±3.41 | 30.34±6.14 29.14±6.39 25.17±8.52 22.95±9.73 |
30 days feeding trials of table 10 slim tea biochemical investigation in latter stage result (x ± s)
| Sex | Group | Number of animals (only) | Glutamic-pyruvic transaminase (U/L) | Glutamic-oxalacetic transaminease (U/L) | Total protein (g/L) | Albumin (g/L) |
| Male | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 29.13±3.22 27.65±7.67 30.43±4.60 27.01±6.30 | 105.81±21.03 109.23±11.77 96.14±22.36 102.85±18.49 | 71.50±2.28 71.70±4.32 71.10±3.31 71.80±1.37 | 36.10±1.95 38.04±3.57 36.82±2.56 36.42±2.52 |
| Female | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 26.40±4.07 27.40±5.85 26.73±6.07 25.95±6.27 | 101.30±17.83 101.02±22.52 98.93±19.68 106.42±16.70 | 71.70±3.02 72.70±4.19 72.10±4.24 71.80±3.09 | 38.80±3.09 38.70±2.58 37.52±2.89 37.75±3.64 |
Continuous 30 days feeding trials of table 10 slim tea biochemical investigation in latter stage result (x ± s)
| Sex | Group | Number of animals (only) | Cholesterol (mmol/L) | Triglycerides (mmol/L) | Urea nitrogen (mmol/L) | Serum creatinine (μ mol/L) | Blood sugar (mmol/L) |
| Male | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 1.83±0.27 1.80±0.33 1.82±0.30 1.77±0.23 | 0.70±0.18 0.76±0.27 0.72±0.19 0.77±0.23 | 4.29±0.41 4.35±0.49 4.12±0.72 4.33±0.38 | 36.30±3.58 37.20±3.88 31.81±6.34 35.35±2.48 | 3.59±0.50 3.66±0.51 3.51±0.93 3.65±0.59 |
| Female | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 1.90±0.36 1.92±0.27 1.99±0.32 1.96±0.29 | 0.76±0.32 0.73±0.22 0.62±0.18 0.75±0.13 | 4.61±0.70 4.62±0.64 4.30±0.44 4.36±0.74 | 37.17±4.44 39.49±2.69 35.46±3.60 37.86±4.14 | 3.88±0.93 3.66±0.41 3.78±0.44 3.71±0.27 |
Table 11 slim tea is to the influence of the dirty body ratio of rat
| Sex | Group | Number of animals (only) | Liver/body (%) | Spleen/body (%) | Kidney/body (%) | Testis/body (%) |
| Male | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 3.44±0.23 3.39±0.23 3.49±0.35 3.43±0.20 | 0.34±0.04 0.32±0.02 0.33±0.03 0.33±0.03 | 0.73±0.06 0.73±0.04 0.74±0.06 0.74±0.05 | 0.91±0.04 0.92±0.04 0.92±0.06 0.91±0.05 |
| Female | Dosage group high dose group in the control group low dose group | 10 10 10 10 | 3.28±0.27 3.33±0.26 3.26±0.18 3.29±0.29 | 0.32±0.02 0.32±0.02 0.32±0.03 0.32±0.04 | 0.75±0.05 0.75±0.06 0.77±0.06 0.75±0.05 | ---- ---- ---- ---- |
Table 12 liver organization pathological examination result
| Pathology | The number of animals of pathology appears in male mouse | The number of animals of pathology appears in female mouse | |||
| High dose group | Control group | High dose group | Control group | ||
| Liver cell | The degeneration necrosis cell infiltration | 0/10 2/10 0/10 | 0/10 1/10 0/10 | 1/10 1/10 0/10 | 1/10 1/10 0/10 |
| Between matter | Blood vessel dilatation cell infiltration fibrillatable | 0/10 0/10 0/10 | 0/10 1/10 0/10 | 0/10 1/10 0/10 | 0/10 1/10 0/10 |
| Other | 0/10 | 0/10 | 0/10 | 0/10 | |
Attention: pathology (as follows) appears in 1 animal in 10 animals of 1/10 expression.
Table 13 spleen tissue pathological examination result
| Pathology | The number of animals of pathology appears in male mouse | The number of animals of pathology appears in female mouse | |||
| High dose group | Control group | High dose group | Control group | ||
| White pulp | The degeneration necrosis cell infiltration | 0/10 0/10 0/10 | 0/10 0/10 0/10 | 0/10 0/10 0/10 | 0/10 0/10 0/10 |
| Red pulp | The degeneration necrosis cell infiltration | 0/10 0/10 0/10 | 0/10 0/10 0/10 | 0/10 0/10 0/10 | 0/10 0/10 0/10 |
| Other | 0/10 | 0/10 | 0/10 | 0/10 | |
Table 14 renal tissue pathological examination result
| Pathology | The number of animals of pathology appears in male mouse | The number of animals of pathology appears in female mouse | |||
| High dose group | Control group | High dose group | Control group | ||
| Glomerulus | The degeneration necrosis cell infiltration | 0/10 0/10 0/10 | 0/10 0/10 0/10 | 0/10 0/10 0/10 | 0/10 0/10 0/10 |
| Renal tubule | The degeneration necrosis cell infiltration | 1/10 0/10 0/10 | 0/10 0/10 0/10 | 1/10 0/10 0/10 | 1/10 0/10 0/10 |
| Between matter | Blood vessel dilatation cell infiltration fibrillatable | 0/10 1/10 0/10 | 0/10 2/10 0/10 | 0/10 0/10 0/10 | 0/10 2/10 0/10 |
| Other | 0/10 | 0/10 | 0/10 | 0/10 | |
Table 15 gastric tissue pathological examination result
| Pathology | The number of animals of pathology appears in male mouse | The number of animals of pathology appears in female mouse | |||
| High dose group | Control group | High dose group | Control group | ||
| Mucous membrane | Congestion and edema hemorrhagic necrosis ulcer | 0/10 2/10 0/10 0/10 0/10 | 0/10 2/10 0/10 0/10 0/10 | 0/10 1/10 0/10 0/10 0/10 | 0/10 1/10 0/10 0/10 0/10 |
| Between matter | The proliferation of fibrous tissue of blood vessel dilatation cell infiltration | 0/10 1/10 0/10 | 0/10 0/10 0/10 | 0/10 1/10 0/10 | 0/10 1/10 0/10 |
| Other | 0/10 | 0/10 | 0/10 | 0/10 | |
Table 16 duodenum histopathological examination result
| Pathology | The number of animals of pathology appears in male mouse | The number of animals of pathology appears in female mouse | |||
| High dose group | Control group | High dose group | Control group | ||
| Mucous membrane | Congestion and edema hemorrhagic necrosis ulcer | 0/10 2/10 0/10 0/10 0/10 | 0/10 1/10 0/10 0/10 0/10 | 0/10 1/10 0/10 0/10 0/10 | 0/10 1/10 0/10 0/10 0/10 |
| Between matter | The proliferation of fibrous tissue of blood vessel dilatation cell infiltration | 0/10 1/10 0/10 | 0/10 1/10 0/10 | 0/10 1/10 0/10 | 0/10 1/10 0/10 |
| Other | 0/10 | 0/10 | 0/10 | 0/10 | |
The female mouse ovary tissue of table 17 pathological examination result
| Pathology | The number of animals that pathology occurs | ||
| High dose group | Control group | ||
| Follicular development | Well generally poor | 10/10 0/10 0/10 | 10/10 0/10 0/10 |
| Corpus luteum, lean type, corpus medullae | The downright bad cell infiltration of blood vessel dilatation cell degeneration | 0/10 0/10 0/10 0/10 | 0/10 0/10 0/10 0/10 |
| Other | 0/10 | 0/10 | |
The male mouse testis tissue of table 18 pathological examination result
| Pathology | The number of animals that pathology occurs | ||
| High dose group | Control group | ||
| The convoluted seminiferous tubule seminaferous epithelium | The degeneration necrosis cell infiltration | 0/10 0/10 0/10 | 0/10 0/10 0/10 |
| Between matter | Blood vessel dilatation oedema cell infiltration | 0/10 0/10 0/10 | 0/10 0/10 0/10 |
| Other | 0/10 | 0/10 | |
Table 19 blood routine and biochemical indicator detect
| Test item | The example number | Before the test-meal | After the test-meal |
| Total protein (g/L) albumin (g/L) glutamic-pyruvic transaminase (U/L) urea (mmol/L) blood sugar (mmol/L) creatinine (umol/L) uric acid (umol/L) T-CHOL (mmol/L) triglycerides (mmol/L) hemoglobin (g/L) red blood cell count(RBC) (* 1012/ L) white blood cell count(WBC) (* 10 9/L) | 32 32 32 32 32 32 32 32 32 32 32 32 | 71.3±3.6 45.1±4.7 27.8±8.1 4.3±1.0 4.6±0.6 103.1±24.3 222.2±71.9 4.4±1.2 1.2±0.6 118.9±18.8 4.1±0.5 6.4±2.2 | 71.1±2.2 44.0±3.3 28.4±6.0 4.5±0.9 4.8±0.5 98.2±23.9 240.3±81.5 4.8±0.5 1.4±0.5 128.3±16.7 4.3±0.4 7.3±1.9 |
Claims (7)
1. a slim tea is characterized in that being made up of by weight following ingredients: green tea: 5.9-6.1 part, honeysuckle: 3.9-4.1 part, cassia seed: 2.9-3.1 part, lotus leaf: 2.9-3.1 part, gynostemma pentaphylla 1.4-1.6 part, hawthorn: 1.4-1.6 part, honey: 5.9-6.1 part.
2. according to the described health protection tea of claim 1, it is characterized in that: described green tea is selected from green tea more than three grades.
3, health protection tea according to claim 1 and 2 is characterized in that: by following ingredients by weight forming: green tea: 6 parts, honeysuckle: 4 parts, cassia seed: 3 parts, lotus leaf: 3 parts, 1.5 parts of gynostemma pentaphylla, hawthorn: 1.5 parts, honey: 6 parts.
4. prepare the method for the described health protection tea of claim 1, may further comprise the steps:
A prepares burden by prescription, checked the mark in green tea, honeysuckle, cassia seed, lotus leaf, gynostemma pentaphylla, mountain and do not rinse well repeatedly with flowing water, and after oven dry under the 70-90 ℃ of condition, it is standby to pulverize 20 mesh sieves more respectively;
Gynostemma pentaphylla after b will clean, pulverize, hawthorn are put into extractor respectively and extract 2 times; For the first time add 10 times of water, soaked 1.5-2.5 hour, little boiling decocted 1.5-2.5 hour, filtered extract, continued to employ; Add for the second time 6 times of water, little boiling decocted 1.5-2.5 hour, filtered extract, merged extracted twice liquid; The extract vacuum being concentrated into density again is about 1.0-1.2, and the gained concentrate is carried out spray-drying, promptly obtains gynostemma pentaphylla, hawthorn powder respectively, standby;
C mixes the powder of green tea, honeysuckle, cassia seed, lotus leaf with step b gained gynostemma pentaphylla, hawthorn powder powder, evenly stirred 4 hours in agitator, gets mixture of powders;
In the mixture that d is obtained, and add water even stirring 3.5-4.5 hour in agitator that weight is honey amount 1/4 in step c; Get particulate mixtures;
E crosses 5 mesh sieves with steps d gained particulate mixtures, gets hybrid fine particles;
F is dried hybrid fine particles 1-2 hour under 80 ℃ of conditions, and moisture is reduced to below 4%;
Hybrid fine particles after g will be dried is distributed into bag on demand, adopts radiosterilization then, makes qualified health protection tea.
5, according to the preparation method of the described health protection tea of claim 4, it is characterized in that: the bake out temperature among the described step a is 80 ℃, soak time described in the step b is 2 hours, little decocting time that boils is 2 hours for the first time, little decocting time that boils is 2 hours for the second time, it is 1.1 that vacuum concentrates density, and the mixing time of steps d is 4 hours.
6, the preparation method of health protection tea according to claim 4 is characterized in that: described steps d-g carries out in the health cleanliness factor is 100,000 grades dust proof workshop.
7, according to the preparation method of the described health protection tea of claim 4, it is characterized in that: the disinfection way of described step g is 7.5KGYCo
60Radiosterilization.
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