CN1060384C - Tianjing Busuiye medicinal liquid and its preparing method - Google Patents

Abstract

The present invention relates to a medicament having the functions of nourishing kidneys, strengthening yang, engendering essence, boosting marrow and delaying senility and a preparing method thereof. The medicament is prepared from 25 Chinese and Western medicaments comprising calf thymus, calf spleen, auricle of pig's heart, ginseng, sea horse, epimedium herb, psoralea fruit, baical skullcap root, acanthopanax root, solomonseal rhizome, procaine and the like, and mixed components of animal's thymus gland according to certain contents. In preparing method, the Chinese medicaments and the animal medicaments are respectively prepared by different steps, and then each medicament is processed by the steps of proportioning, pH regulation, specific gravity measurement, inspection, sterilization, reinspection, packaging, etc. The present invention provides a compound medicament preparation prepared by modern scientific technologies, and is capable of supplementing five viscera, regulating six pulses, supplementing Qi, nourishing spirit and improving immunologic functions of human bodies.

Description

A kind of antiaging agent oral liquid and preparation method thereof

The invention belongs to the antiaging agent preparation, particularly relate to a kind of antiaging agent oral liquid and preparation method thereof.

The people is to the decline gradually along with the growth at age of later on normal immunologic function of middle age, the immunologic function decline is the main cause of human senility, what come along with the immunologic function decline is to infect, the immunity comprehensive disease, the sickness rate of cancer obviously increases, and has quickened the human aging process, and these aging phenomenon traditional Chinese medical science are thought deficiency of the kidney, due to the brains deficiency,, benefiting essence and marrow controls antidotal theoretical foundation so just becoming dialectical opinion.At present, the medicine of some the kidney invigorating, health preserving, tonifying YANG is arranged also, the prescription of these medicines is had nothing in common with each other, and also is added with valuable wild animal medicine in the prescription, and these medicines also can tonifying kidney and preserving health, human body is played certain anti-ageing healthcare effect, but the medicine valency is expensive, and function is very not comprehensive.Seldom see the pharmaceutical preparation of the high treatment nephropathy of curative effect that Chinese medicine and western medicine prescription combination and animal thymus blending constituent utilize on the market.

The purpose of this invention is to provide and a kind ofly have kidney invigorating and YANG supporting, spermatogenesis benefit marrow, eliminate senile symptom by what animal thymus blending constituent and Chinese medicine and western medicine prescription were formed, the human body immunity improving function, a kind of antiaging agent oral liquid of delaying senility function and preparation method thereof.

The object of the present invention is achieved like this: a kind of antiaging agent oral liquid, its characteristics are that said composition is to make 8 ~ 30 parts of calf thymuss by the following raw materials by weight proportions thing, 3 ~ 20 parts of calf spleens, auricle is 3 ~ 25 parts on the pig heart, 3 ~ 15 parts of Radix Ginsengs, hippocampus 3 ~ 10 part, 10 ~ 30 parts of Herba Epimedii, 10 ~ 35 parts of Fructus Psoraleaes, 10 ~ 30 parts of Radix Scutellariaes, 10 ~ 30 parts of Rhizoma Polygonatis, 10 ~ 30 parts of Radix Et Caulis Acanthopanacis Senticosis, 10 ~ 30 parts of Radix Rehmanniae, 10 ~ 30 parts of Fructus Lycii, 10 ~ 25 parts of Radix Asparagi, 10 ~ 30 parts of the Radixs Astragali, 8 ~ 25 parts of the Rhizoma Atractylodis Macrocephalaes, 8 ~ 30 parts in Radix Glycyrrhizae, 8 ~ 30 parts of Radix Polygoni Multiflori, 8 ~ 30 parts of the Radix Paeoniae Albas, 8 ~ 30 parts of Radix Codonopsis, 6 ~ 30 parts of Placenta Hominiss, 8 ~ 30 parts of Herba Cistanches, 8 ~ 30 parts of Radix Isatidiss, 0.0003 ~ 0.05 part of procaine, 8 ~ 30 parts of Fructus Ligustri Lucidi, 8 ~ 30 parts of Fructus Schisandrae Chinensis.

A kind of above-mentioned antiaging agent oral liquid is characterized in that material medicine optimum weight proportioning is 30 parts of calf thymuss, 15 parts of calf spleens, auricle is 15 parts on the pig heart, 15 parts of Radix Ginsengs, 6 parts of Hippocampus, 30 parts of Herba Epimedii, 25 parts of Fructus Psoraleaes, 25 parts of Radix Scutellariaes, 25 parts of Rhizoma Polygonatis, 25 parts of Radix Et Caulis Acanthopanacis Senticosis, 15 parts of Radix Rehmanniae, 20 parts of Fructus Lycii, 15 parts of Radix Asparagi, 20 parts of the Radixs Astragali, 15 parts of the Rhizoma Atractylodis Macrocephalaes, 20 parts in Radix Glycyrrhizae, 20 parts of Radix Polygoni Multiflori, 15 parts of the Radix Paeoniae Albas, 25 parts of Radix Codonopsis, 25 parts of Placenta Hominiss, 20 parts of Herba Cistanches, 20 parts of Radix Isatidiss, 0.0006 part of procaine, 20 parts of Fructus Ligustri Lucidi, 20 parts of Fructus Schisandrae Chinensis.

The characteristics of animal thymus of the present invention are: the enhancing human body immunity function, the people is to middle age thymus atrophy gradually later on, become fatty tissue to geratic period thymus, among the human aging process, because the secretory function of thymosin descends, immunologic function also descends thereupon, make human body present aging phenomenon gradually, it is reported thymus transplantation in the human body triangular muscle, can treat immunodeficiency diseases, the human body immunity improving function.Thymosin is that the T cell individual is grown necessary, the forfeiture of T cell often causes autoimmune generation, for being tending towards old and feeble people gradually, extracts the mixing composition (containing the thymosin parahormone) of animal thymus, but the enhancing human body immunity function, and the effect of the senilism of preventing is arranged.There is not obvious species specificity according to newly organized " materia medica " third edition record thymus parahormone.

Spleen is the immune device of human body maximum, the cell that spleen produced has the effect of engulfing antibacterial, virus and harmful substance, the traditional Chinese medical science is thought with the dirty effect that the human body immunity improving function is arranged of dirty benefit, extracts the spleen liquid factor, and being used to strengthen immune function of human body is the theory that meets Chinese medicine.

The auricle of pig contains a kind of heart factor, for old and feeble gradually people, extracts the heart humoral factor, is applied to the irritability that human body can increase heart, improves cardiac function.

Have effects such as " tonifying five ZANG-organs ", " peace spirit " according to ancient book " herbal guiding principle wood ", " book on Chinese herbal medicine converges and says ", Shennong's Herbal record Radix Ginseng, above-mentioned calf thymus, calf spleen, three kinds of animal drugs of Cor Sus domestica ear are equipped with the merit that Radix Ginseng, Hippocampus, Herba Epimedii, Fructus Psoraleae, Radix Scutellariae, Radix Et Caulis Acanthopanacis Senticosi, Rhizoma Polygonati have YIN nourishing and YANG strengthening, heat-clearing and toxic substances removing, tonifying five ZANG-organs, peace spirit, play an important role for the root-kidney yang of the human body of bestirring oneself gas, but Hippocampus one medicine can see that disease can reach the function of blood circulation promoting competent silt and heat-clearing and toxic substances removing to common old and feeble blood stasis with warm promotion whole body.The ancillary drug Radix Rehmanniae of this prescription principal agent, Fructus Lycii, Radix Asparagi, the Radix Astragali, the Rhizoma Atractylodis Macrocephalae, Radix Glycyrrhizae, Radix Polygoni Multiflori have invigorating the liver and kidney promoting generation of vital essence and blood, Yin-nourishing and body fluid promoting, cooling blood for hemostasis, reducing the asthenic fever effect.This another group adjuvant Radix Paeoniae Alba of filling a prescription, Radix Codonopsis, Placenta Hominis, Herba Cistanches, Radix Isatidis, procaine, Fructus Ligustri Lucidi, Fructus Schisandrae Chinensis are the YIN nourishing of enriching blood, nourishing YIN and benefiting blood, the effect of calming the nerves.

The procaine medicine can alleviate the depressed and worried of elderly patients, improving memory and ability of thinking.Has the effect of mediator's somatic nerves function balance.

The preparation that this full formula compatibility is comparatively precise and appropriate has cooperative effect, has no side effect through the zoopery proof.Can replenishing vital QI with drugs of warm nature Rong Wei, tonifying five ZANG-organs, accent six kinds of pulses, grow fill hundred arteries and veins, moist three warmers, invigorate blood circulation help gas, reach that QI invigorating is reposed, the merit of YIN nourishing and YANG strengthening, heat-clearing and toxic substances removing and benefiting essence and marrow, and can human body immunity improving function, slow down aging play main effect, old people's deficiency in both YIN and YANG, QI and blood impassable patient are suited.

The main Chinese medicinal materials source is: Radix Ginseng is produced in northeast, Hippocampus is produced in Guangdong, the Herba Epimedii stem and leaf is produced in Shanxi, scutellariae,radix is produced in northeast, the Fructus Psoraleae fruit is produced in Sichuan, the compound preparation that 25 kinds of Chinese herbal medicine such as Xinjiang calf animal thymus, procaine, animal drugs, Western medicine are formed, drug dose are all stipulated by (pharmacopeia nineteen ninety-five first one).

This medicine is a yellowish-brown liquid, sweet, little hardship of distinguishing the flavor of, and every bottle 250 milliliters * 1 bottle, every milliliter contains 1 part of crude drug in whole.Oral one time 10 milliliters, a twice-daily, (medicine) being taken before meal is sooner or later taken medicine and is avoided strong tea in back 2 hours, if any precipitation, takes after shaking up, and curative effect is not had influence.Nontoxic, the side effect of this product.Want lucifuge, airtight, shady and cool place to preserve.

A kind of preparation method of above-mentioned antiaging agent oral liquid, be characterized in: selected first-class medical material clean → was pulverized 40 mesh sieves, take by weighing: 3 ~ 15 parts of Radix Ginsengs, hippocampus 3 ~ 10 part, 10 ~ 30 parts of Herba Epimedii, 10 ~ 35 parts of Fructus Psoraleaes, 10 ~ 30 parts of Radix Scutellariaes, 10 ~ 30 parts of Rhizoma Polygonatis, 10 ~ 30 parts of Radix Et Caulis Acanthopanacis Senticosis; 10 ~ 30 parts of Radix Rehmanniae, 10 ~ 30 parts of Fructus Lycii, 10 ~ 25 parts of Radix Asparagi, 10 ~ 30 parts of the Radixs Astragali, 8 ~ 25 parts of the Rhizoma Atractylodis Macrocephalaes, 8 ~ 30 parts in Radix Glycyrrhizae, 8 ~ 30 parts of Radix Polygoni Multiflori; 8 ~ 30 parts of the Radix Paeoniae Albas, 8 ~ 30 parts of Radix Codonopsis, 6 ~ 30 parts of Placenta Hominiss, 8 ~ 30 parts of Herba Cistanches, 8 ~ 30 parts of Radix Isatidiss, 8 ~ 30 parts of Fructus Ligustri Lucidi, 8 ~ 30 parts of mercerations of Fructus Schisandrae Chinensis bubble 24 hours → (the changzhou second chemical mechanical equipment factory makes to put into multi-function extractor, model ON type 200V) water is carried 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 deal by every milliliter and add distilled water after quiet straight 24 hours → vacuum filtration → quiet straight 24 hours final vacuum sucking filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.The medicine that will contain volatile oil, vacuum extraction volatile oil will extract in medicinal residues and the straight in the lump extraction pot of said medicine then in advance.The volatile oil that extracts stocked wait to join, with the calf thymus of getting ready, the calf spleen, on the pig heart auricle in advance 8 ~ 20 ℃ of one weeks of stored frozen to reach the purpose of handling autolytic ferment → remove connective tissue and impurity → clean → 8 ~ 30 parts of weight calf thymuss of slurrying (rubbing) → take by weighing with electrical meat mincer with distilled water, 3 ~ 20 parts of calf spleens, the normal saline of 3 ~ 25 parts → adding of auricle doubling dose leaches on the pig heart, soak earlier and soaked filtration in 0.5 hour (can soak as the case may be 2 ~ 3 times) → made medicinal liquid slightly be chilled to 40 ~ 60 ℃ in 3 ~ 5 minutes the rapid heated and boiled of filtrate at 75 ~ 80 ℃ again in 1 hour and use 6.804 kilograms of pressure through 30 minutes heat treated immediately with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out with paper pulp cake sucking filtration → filtrate at room temperature, filter with frozen water cooling reuse paper pulp cake then, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, the Pu Lu point is because of part adapted O.0003 ~ 0.05, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.

The excellent beneficial effect of antiaging agent oral liquid is that treatment is suffered from a deficiency of the kidney, the kidney invigorating, QI invigorating, help the effect of intensive culture god, YIN nourishing, tonifying YANG and heat-clearing and toxic substances removing.Improve human body reticuloendothelial system phagocytic antibacterial, the ability of virus and harmful substance through experiment confirm; Improve the function of human body cell inducement interferon; Nervous system there are balance and regulating action; The stem cell that bone marrow is produced is transformed into the T lymphocyte, thereby can strengthen and improve immune function of human body.Sick evident in efficacy for brain function decline, neurasthenia, the tinnitus of having a dizzy spell etc., memory reinforcing; Diseases such as sexual hypofunction, climacteric syndrome, removing lipofuscin (senile plaque), soreness of the waist and knees, rheumatoid arthritis, hepatitis also there is significant curative effect; Can promote fracture heals in early days, postcerebral injury syndrome, the patient who weakens for after the wound and operation back resistance is more remarkable.

Put to the proof below by relevant test: one, slow down aging and the clinical observation of treatment insufficiency of kidney-YANG

Anti-old (energy) three essentials-essence theory and the Immunization Update theory paid attention to of inventor's relevant health preserving from ancient medical book (health preserving side), 25 kinds of Chinese medicine and western medicine, the animal drugs of screening, through clinical 26 years research, practice, utilize the refining compound preparation that forms of modern science technology.Through clinical to 80 examples, the senile symptom patient suffers from a deficiency of the kidney, be divided into antiaging agent oral liquid group (treatment group) at random, sucrose solution placebo group (matched group), adopt the double blind control method, took medicine three months, observed result shows: the antiaging agent oral liquid can make the total mark value decline (p＜0.01) of elder kidney asthenia senile symptom.Visual acuity and audition improve, and heart rate shortens between often again, and memory improves.Superoxide dismutase in the erythrocyte (SOD) vigor raises (p＜0.01), and blood plasma lipid peroxide (LPO) content descends; Comparing with matched group all has significant difference (p＜0.01).Prompting reduces the free radical poisoning injury, strengthens anti-oxidant vigor, originally can alleviate the total effective rate 94% of senile symptom.In order further to confirm its clinical effect, the same period, between year March nineteen ninety-five to 1997, we adopted the double blind control method, carry out second phase paired observation to there not being the elder kidney asthenia patient of serious organic disease, and acquisition data 80 examples now are reported as follows the result.

1, clinical data

The patient is from Wusu City, Xinjiang institute of traditional Chinese medicine, Yi Li continent Kuitun hospital and Yi Li continent Kuytun City hospital.In the second phases 80 example, male's 60 examples, women's 20 examples at 60 ~ 80 years old age, are divided (treatment group) 50 examples, mean age 68,11 ± 5,31 years old, sucrose solution placebo group (matched group) 30 examples at random.

2, the diagnostic criteria reference literature is formulated (1):

(1) geratic period 〉=60 year old, (2) are selected to look into (comprising electrocardio, hematuria routine, liver, renal function, blood pressure detection etc.) through visiting, prove be not in the mood for, organic disease persons such as brain, liver, kidney and endocrine.

3,14 senile symptoms are kept the score with the dynamic change classification: reference literature is formulated relevant all types of dialectical standards and the senile symptom integrated value evaluation criteria of suffering from a deficiency of the kidney in (1).Soreness of the waist and knees, lassitude and weak, the cold syndrome of the stomach cold extremities, the nocturia frequency, spontaneous perspiration is breathed hard, and big loose stool is thin, and alopecia or poliosis are forgetful, luxated tooth, hyposexuality, tinnitus, dizzy, sleep not good enough or insomnia.

Possess insufficiency of kidney-YANG, the deficiency of kidney-QI binomial, in three of minor symptoms and the tongue arteries and veins one all scores according to integration method.

4, senile symptom integrated value assessment method: the symptom note that initiatively say (1) 4 minutes.(2) remarkable or lasting note 3 minutes that occur of the symptom of getting.Heavy or be interrupted the symptom note that occurs 2 minutes when light when (3) getting.(4) get gently or the symptom note that occurs once in a while 1 minute.(5) asymptomatic note is 0 minute.(6) any person of appearance all is designated as 6 fens in the tongue arteries and veins.

5, medicine and method of administration: reference literature is formulated (2), adopts the randomized controlled method, and oral antiaging agent oral liquid 20ml/ day is organized in treatment, divides empty sooner or later clothes respectively to obey 10ml.Matched group clothes sucrose solution placebo, dosage and the method same with the treatment group, each group serve on three months.

6, health check-up method:

(1) intelligence is measured: comprise and point to memory and learning by association, 1. point to the test of memory method: after the experimenter listened to the content in the recorder and requires, it at first answered out " sensings " content (120s prescribes a time limit).Answer out non-" sensing " content (120s in limited time) again.The standard of keeping the score: whenever answered questions a speech note 1 minute, and answered wrong and answer button 1 minute, inerrancy full marks (pointing to and non-sensing) respectively 12 minutes more.2. the testing method of learning by association: listen to capstan, the standard of keeping the score is, " answers " two kinds with " answering difficulty " in the problem of answering easily, whenever answers questions a speech of " easily " and remembers 0.5 fen, whenever answer questions speech note 1 minute of " difficulty ", answer be 9 minutes to full marks entirely.

(2) look adjusting power (visual acuity): for carrying out after the calibrated vision of ametrope, write down the myopia point and the High eye point of images of left and right eyes respectively, calculated difference is visual acuity (cm).

(3) audition: respectively from 250Hz, 4000Hz, the ascending and descending adjusting input of signal generator ab amount can the amount of listening be that audition value (dB) is calculated with minimum ab amount.

(4) postexercise heart rate assay method recovery time: squat upright movable 10 times to 20 times, write down its postexercise heart rate required time (S) when returning to normal cardiac rate respectively.

(5) the one retraction time of skin pull-up: inboard 3cm is the trial zone on the former wrist: let go after 1 second with thumb, forefinger pull-up skin, write down it with stopwatch and calm down the time (S).

7, lab testing: (1) routine blood test, (2) routine urinalysis and glucose in urine are qualitative, (3) liver function, (4) serum urea nitrogen (Buw).(5) plasma lipid peroxide (LPO).(6) surpass oxide enzyme (SOD) in the erythrocyte.(7) glutathione peroxidase in the whole blood (GSH-Px) measure, LPO adopts Yagi spark gap state husband method (3), SOD adopts the nitrite forming method, GSH-Px employing Hafeman method.

The result

8, efficacy assessment standard: reference literature is formulated (1).

(1) produce effects: 1. the aging of treatment back sees that the disease integration descends more than 2/3,2. takes a turn for the better more than 2 in 1 ~ 5 of the health check-up.3. 3 improvements in 4 ~ 7 of the lab testings.

(2) effective: 1. treatment back aging sees that the disease integration descends 1/3.2. 2 improvements in 1 ~ 5 of the health check-up.3. 2 improvements in 4 ~ 7 of the lab testings.

(3) invalid: as fail to reach above-mentioned effective standard, or lab testing to have 2 (and more than 2) persons of increasing the weight of.

9, two groups of curative effects relatively see Table 1:

Table 1 liang group curative effect relatively

The group example	Produce effects	Effectively	Invalid	Effectively total
					Not several	Example number %	Example number %	Example number %	％
Treatment group 50	22 44	25 50	3 6	94
					Matched group 30	0 0	8 26．7	22 73．3	26

After the treatment group clothes antiaging agent oral liquid, its aging sees that the disease integration analyzes through Ridit, all significantly reduce (p＜O.01).And difference is not remarkable before and after the matched group placebo treatment.

10, physical examination results change:

(1) no significant change before and after the treatment of blood pressure, pulse treatment group, illustrate this product to nothing obviously influence.

(2) intelligent measurement result: the treatment group is pointed to has highly significant to improve (p＜0.01) after memory is treated with learning by association, and no significance changes before and after the treatment of control group.

(3) visual accommodation (visual acuity) result: treatment group left eye (p＜0.01) right eye (p＜0.05).And matched group does not have significant change.

(4) audition treatment back result: left and right ear significantly improves (p＜0.01) before treating, and matched group does not have significant change.

(5) postexercise heart rate measurement result recovery time: heart rate has obvious shortening (p＜0.01) between often again before and after upright movable 10 to 20 the treatment groups treatment of squatting.And matched group has the prolongation of different time, but the statistics there was no significant difference.

(6) skin pull-up retraction time result: two groups of treatment back differences compare (p＜0.01), illustrate that treatment group treatment back skin elasticity strengthens, and matched group is no change then.

11, blood, routine urinalysis and hepatic and renal function check result: the treatment front and back are all in normal range.Part patient has surveyed, cholesterol, blood glucose, equal no change before and after taking medicine.

12, blood plasma LPO before and after the treatment, SOD in the erythrocyte, whole blood GSH-Px changes: the treatment group, compare before and after the treatment, blood plasma LPO content decline (p＜0.01), erythrocyte sod is active to rise (p＜0.01), and more all has significance to change (p＜0.01) according to group, no significance variation before and after the treatment of control group.

Table 2 liang group blood plasma LPO content, erythrocyte sod, whole blood GSH-Px vigor comparison (xx ± S)

Group	The example number	LPO(mmol／ml)	SOD(ug／g．Hb)	GSH-PX (unit of activity)
					The treatment group	Treat preceding 50	4．49±0．71	488．51±46．46	58．82±8．02
Treatment back 49	3．27±0．52※	571．35±95．54※	61．95±6．62
				Matched group		Treat preceding 30	4．48±0．72	489．89±57．40	61．09±8．60
Treatment back 26	4．23±0．62	503．35±43．78	62．93±7．14

Annotate: with preceding relatively ※ p＜0.01 of treatment; Compare △ p＜0.01 with matched group.

13, side effect: do not find any side effect after the treatment.

Conclusion

The traditional Chinese medical science is thought, kidney controlling essence storage, and with people growth, grow old and feeble closely relatedly, kidney qi is contained the then longevity, and kidney qi declines and then dies young.Therefore the kidney invigorating is an important channel of life lengthening, and this paper is discussed below its effect by the clinical observation to the defying age medicine oral liquid:

(1) these product can make the aging of suffering from a deficiency of the kidney see that the disease integrated value obviously descends, and are especially improving aspect the insufficiency of kidney-YANG pattern of syndrome more significantly, illustrate that this side has slow down aging, the kidney invigorating effect.

(2) the old and feeble patient that suffers from a deficiency of the kidney takes that SOD obviously raise when LPO descended in blood after the antiaging agent oral liquid, illustrates that these product reduce the effect of radical pair body damage, may be by improving the vigor realization of peroxidase.

(3) these product can improve brain intelligence, memory reinforcing, and the raising of visual acuity and audition, heart rate shortens between often again.

The effect that to sum up every antiaging agent oral liquid has the kidney invigorating and removes old people's senile symptom has slow down aging, removes the effect of free radical.

List of references

1, Chen Guiting, etc., up-to-date domestic and international disease treatment standard, Xueyuan Press, 1 edition, Beijing, 1991:1416,1418 (the screening rules of slow down aging Chinese medicine and clinical observation standards).

2, Du Xin, etc., vigor Soviet Union anti-aging effects research-507 routine double blind control methods are observed, combination of Chinese and Western medicine magazine, 1986,6 (5): 271.

3, Yagi spark gap state husband, serum is crossed the clinical meaning of acidify lipid assay と body, clinical pathology, 1981,29 (3): 221.

Two, the clinical observation of antiaging agent oral liquid slow down aging

To 42 examples, the clinical observation result of healthy elderly shows through clinical:

(1) can obviously increase the total E rosettes of old people (ETRFC) percentage rate (P＜0.01) and T-lymhocyte transformation rate (P＜0.05).Has the effect that improves the body ability to work and eliminate old symptom.

(2) have the peripheral leukocytes sum of increasing and neutrophil cell (P＜0.01),, promote the effect of cellular immunization and humoral immunization, slow down aging is played one of main mechanism to strengthen specific immunity.

Object and method

1, the selection of object: the patient is from Wusu City, Xinjiang institute of traditional Chinese medicine, Yi Li state, Xinjiang Kuitun hospital, Yi Li state, Xinjiang Kuytun City hospital, by (Gerontological Society of Chinese Medical Association required standard (1)), selection proves through clinical examination (comprising inspections such as electrocardio, hematuria routine, liver function, kidney merit, blood pressure) and is not in the mood for, brain, lung kidney and in organic disease person's 42 examples such as branch oozes, male's 27 examples wherein, women's 15 examples, 60 ~ 80 years old age, and the mean age 68.03 ± 5.31 (x ± SD).Be divided into antiaging agent oral liquid group (treatment group) 36 examples at random, sucrose solution placebo group (matched group) 6 examples.

2, take medicine and Therapeutic Method: adopt the double blind control method, reference literature (2 ~ 4), oral antiaging agent oral liquid 20ml/ day is organized in treatment, divides sooner or later each 10ml on an empty stomach.Matched group clothes sucrose solution placebo, dosage and the method same with the treatment group, each organizes one month is a course of treatment, observes two courses of treatment altogether.Antiaging agent oral liquid and sucrose solution placebo provide by the little pharmaceutical factory of Wusu City, Xinjiang institute of traditional Chinese medicine.

3, observation index: comprise clinical symptoms, physical examination, generally chemical examination and test on immune function.

(1) clinical symptoms: giddy, fatiguability, uncomfortable in chest, five symptoms relevant such as sleep is not good enough, inappetence with aging.

(2) general chemical examination: comprise white blood count and differential, routine urinalysis regulating liver-QI kidney function test.

(3) immunologic function test: comprise determination of immunoglobulin, the lysozyme quantitative assay, with tritium mark thymidine ( ³H-Tdk) mixing algoscopy carries out lymphocyte transformation test and total E rosette.

The result

1, clinical symptoms change: the treatment group still has tired person's 2 exceptions behind clothes antiaging agent oral liquid treatment, all the other symptoms all disappear, and behind the matched group clothes sucrose solution placebo, symptom does not have obvious improve (table 3).

Clinical symptoms before and after table 3 treatment relatively

Group	n	Dizzy	Fatiguability	Uncomfortable in chest	It is not good enough to sleep	Inappetence
							Before the treatment	36	14	7	7	9	3
36	0	2	0	0	0
						Before the treatment		6	3	2	2	1	0
6	0	0	0	0	0

2, the variation of numeration of leukocyte and classification: the total white blood cells of treatment group and neutrophil cell absolute number all are significantly increased after treatment.

Peripheral leukocytes comparison before and after table 4 treatment (x ± SD)

Group	n	Numeration of leukocyte * 10 9/L	The neutrophil cell absolute number
				Back p value is treated in treatment before treating	36	6.700±1.734	3784±1438
36	8.224±1.582 ＜0.01	5034±1503 ＜0.01
			Back p value is treated in contrast before treating		6	5.725±4.170	3817±1290
6	6.650±1.271 ＞0.05	3868±1456 ＞0.05

Immunoglobulin comparison before and after table 5 treatment (x ± SD)

Group	n	IgG(g／L)	IgA(mg／L)	Igm(mg/L)
					Back p value is treated in treatment before treating	36	11.44±2.87	970±450	1070±330
36	14.55±2.28 ＜0.01	930±690 ＞0.05	830±230 ＜0.01
				Back p value is treated in contrast before treating		6	10.28±1.99	970±400	1070±270
6	16.03±0.69 ＜0.01	1600±530 ＜0.05	1120±210 ＞0.05

(p＜0．01)。And matched group behind clothes sucrose solution placebo except that sum slightly increases, the neutrophil(e) granule absolute number does not have significant change (table 4).

3, liver, renal function change: treatment group and matched group are in the treatment liver function, and except that icteric index and thymol turbidity test slightly changed, all the other two (GPT and HBSAg) all belonged to normal or negative, the renal function and the front and back no change of taking medicine.

4, immunoglobulin changes: the IgG of treatment group its measured value after treatment obviously raises (p＜0.01), and IgA and IgM then do not have significant change: but in the matched group, two measured values of IgG and IgA also have significantly and increase, and Igm does not have significant change (table 5).

The variation of table 7 lysozyme: in treatment group and matched group.Bacterium enzymatic determination value after the treatment all has decline, but not statistically significant (table 6).

Lysozyme comparison before and after table 6 treatment (x ± SD)

Group	n	Lysozyme is (mg/L) relatively
			Back p value is treated in treatment before treating	25	7.27±2.50
25	5.93±2.14 ＞0.05
		Back p value is treated in contrast before treating		4	7.60±3.69
4	4.18±1.42 ＞0.05

Lymphocytic comparison before and after table 7 treatment (x ± sD)

Group	n	3H-TdK(SI)
			Back p value is treated in treatment before treating	25	62.77±36.29
25	100.63±71.12 ＜0.05
		Back p value is treated in treatment before treating		4	59.21±42.20
4	52.30±34.19 ＞0.05

5, the variation of the thin breast conversion test of lymph: the stimulation index (Indexofstimulation be called for short SI) of treatment group after treatment obviously increases (p＜0.05), and matched group no change (table 7).

6, the comparison of total E rosette: treatment is organized total E rosettes measured value and is obviously increased (p＜0.01) matched group and then do not have significant change (table 8).

ETRFC comparison before and after table 8 treatment (x ± SD)

Group	N	ET--RFC(％)
			Back P value is treated in treatment before treating	25	0.36±0.07
25	0.50±0.08 ＜0.01
		Back P value is treated in treatment before treating		4	0.42±0.06
4	0.47±0.05 ＞0.05

7, the back side effect of taking medicine: side effect takes place at the period in a medicine and none example of back of taking medicine.

Conclusion

Aging is the basic biological phenomenas of all lived individualities in the life evolution, by molecular biological research, thinks, the functional status of immuning tissue and immunocyte may be an old and feeble key factor of influence.By the clinical observation of antiaging agent oral liquid, its effect is discussed below:

1, improve the defying age ability of body: old and feeble clinical symptoms, this group have 40 examples time, in treatment back 2 middle of the month, 38 example time complete obiterations (95%) are arranged.

2, to the influence of nonspecific immunity: numeration of leukocyte and the neutrophil cell absolute number of treatment group after treatment has obviously and increases (P＜0.01) matched group and then do not have obvious variation.Be expected this medicine radioactivity, chemical, medicine and the caused leukopenia of other reason are had certain curative effect.

3, to the influence of specific immunity:

(1) influence of pair cell: the conversion test of T lymphocyte, the stimulation index of treatment group after treatment significantly increases (P＜0.05), and matched group does not then have significant change.In addition, total E rosette proves, treatment group its measured value after treatment also obviously rise (P＜0.01) prove that the antiaging agent oral liquid has the effect of promotion cellular immune function.

(2) to the effect of humoral immune function: the main effect of B cell is to produce immunoglobulin.Observed result shows that the antiaging agent oral liquid can make the measured value of IgG significantly increase (P＜0.01), illustrates that the antiaging agent oral liquid has the function that strengthens humoral immunization by the B cell.

To sum up every, the antiaging agent oral liquid can be used as slow down aging, and immune adjuvant is applied to clinical.

List of references

(1) Yang Zeyan, etc., several immune indexes measurement results of healthy elderly.China's geriatrics magazine 1984; 3 (4): 193.

(2) Zhou Wenquan, etc., the screening rules of slow down aging Chinese herbal medicine and clinical observation standard.Combination of Chinese and Western medicine magazine.1986；6<11>：682。

(3) Zhou Shangren compiling: immunodeficiency diseases, 1 edition, Tianjin science tech publishing house; Tianjin, 1981,367.

(4) Du Xin, etc., vigor Soviet Union anti-aging effects research-507 routine double blind control methods are observed.Combination of Chinese and Western medicine magazine.1986；6(5)：271。

Three, the antiaging agent oral liquid is to benefiting essence and marrow, YIN nourishing, tonifying YANG pharmacodynamics research report

The compound recipe Chinese-western medicine preparation antiaging agent oral liquid of nourishing kidney yin and warming and recuperating the kidney-YANG is grown the sex character of castrating cock all optimum effect.And the observation of some leading indicators is shown nourishing kidney yin and the kidney yang sex hormone level in the body that is significantly increased, the mensuration of periphery physiochemical indice is significantly improved.

Select antiaging agent oral liquid the kidney invigorating side medicine for use, the internal relation of the theoretical material base of the pharmacodynamic action of research nourishing kidney yin and warming and recuperating the kidney-YANG and the traditional Chinese medical science " kidney storing essence, living marrow ".

We are model with the castrating cock, are promoting castrating cock sex character to grow, hematosis, and the mechanism that improves hemoglobin, and the observation of contrasting of aspects such as drug effect after effect, preliminary report of results is as follows.

1, materials and methods: (1) cock source: buy 80 days healthy male leghorn of brood birth 25 (average weight 85og) in May, 1996 from breed center, North SinKiang, Wusu City, Xinjiang and take action the quality testing epidemic disease after 1 week through this city veterinary station, the row male castration.

(2) castrating cock operation method: between 1-2 free ribs of cock back, cut long mouthful of 2-3cm, separate the abdominal cavity mucosa, sew up after taking out a side testis, allow it recover to take out the opposite side testis with method after 5 days.Pathological section turns out to be the testis tissue of undeveloped mature.The half-castrate person of cutting only takes out one side testis.

(3) experiment administration group and medication: cock castrating back the 40th day, the cock that will meet complete castration requirement is divided into antiaging agent oral liquid (the kidney invigorating group) and castrating matched group (claiming full castrating group) at random.And get brood uncastrated cockerel in addition as the normal control group.Every group 5-7.

The kidney invigorating group, the antiaging agent oral liquid adult who is provided by the little pharmaceutical factory of Wusu City, Xinjiang institute of traditional Chinese medicine measures 20 times of feed cocks, successive administration 60 days.Normal group and cut group entirely and will not administration handle, each conventional raising 60 days.

2, observation index: observed respectively and the preceding cock body weight of administration, cockscomb height, surface area and external appearance characteristic in 1-2 months after the administration.Administration 2 months and drug withdrawal were done the peripheral hemogram analysis respectively in 2 months, and did serum sex hormone level and measure the hemoglobin group analysis.

3, experimental result:

(1) castrating cock surface changes: castrate the back cock after routine is raised 39 days, and the obvious atrophy of cockscomb, formalness and behavior characteristics are obviously feminized, its body weight, cockscomb surface area and the percentile variation of cockscomb high growth see Table 9.

Each group of table 9 experiment back is hung down cockscomb surface area rate of increase than (m ± SD)

Group (only)	Body weight (%)	Cockscomb surface area (%)	Cockscomb height (%)
				Castrate group 16 entirely	64．1±22．1	—16．8±27．5※	—1．2±22．9※
Half-castrate group 5	64．8±25．3	71．4±4．20	26．6±18．5
				Normal group 4	51．9±19．5	101．8±48．3	48．1±20．0

Annotate: ※ and normal group be P＜0.01 relatively.

(2) cock sex character is grown and the variation of surface after the administration: after the administration 30 days, 60 days, and the variation of surveying body weight, cockscomb height, cockscomb surface area and surface respectively.The result: after the administration cock body weight with castrate relatively no significant difference of group entirely.See Table 10 and table 11 show.

Respectively organize relatively (m ± SD) of cockscomb height change after table 10 administration

Group (only)	Before the administration (cm)	After the administration 30 days (cm)	After the administration 60 days (cm)
				Castrate group 5 entirely	3．30±0．69	3．74±1．38	4．18±1．11
The kidney invigorating group 7	3．73±0．46	6．01±0．68※	7．20±0．68※
				Normal group 4	5．07±0．25※	7．43±0．78※	8．75±1．46※

Annotate: 60 days several 5 of detection chickens (table 11 together) after the administration of the kidney invigorating group

※ with castrate relatively P＜0.01 of group entirely.

Respectively organize (m ± SD) of cockscomb surface area ratio after table 11 administration

Group (only)	Before the administration (cm)	After the administration 30 days (cm)	After the administration 60 days (cm)
				Castrate group 5 entirely	8．92±1．62	9．45±5．17	13．26±4．75
The kidney invigorating group 7	13．89±2．75	33．79±3．97※	39．51±23．86※
				Normal group 4	25．76±3．87※	47．49±10．08※	72．94±13．19※

※ with castrate relatively P＜0.01 of group entirely.

Table 12 is taken medicine and was respectively organized the peripheral blood index in 60 days and change (m ± sD)

Group (only)	Hemoglobin (g%)	Reticulocyte (%)	RBC number (%)
Full E group 5	15．23±0．92	0．40±0．49	5．62±0．45
The kidney invigorating group 7	13．58±4．20	1．16±0．68	5．35±0．81
Normal group 4	17．06±1．20※	2．00±1．40	6．84±1．40

Annotate: with castrate relatively P＜O.06 of group entirely.

The growth of 30 days the kidney invigorating group cockscomb height and surface area all is higher than and castrates group (P＜0.01) entirely but more remarkable in the growth of administration cockscomb after 60 days after the administration.

The variation of cock surface shows: the kidney invigorating group disposition is active, and is how moving, identical with normal group, castrates group entirely and then have a sweet temper torpescence.The kidney invigorating group feather light, plentiful: as to castrate the group feather entirely and loosen tarnish.The kidney invigorating group cockscomb is upright plump the same with normal group scarlet.Castrate group cockscomb atrophy pinkiness entirely, behavior exactly likes hen especially.

Table 13 drug withdrawal was respectively organized the peripheral blood index in 60 days and is changed (m ± sD)

Group (only)	Hemoglobin (g%)	Reticulocyte (%)	RBC number (%)
				Castrate group 5 entirely	14．40+1．90	1．60+1．35	5．83+O．92
The kidney invigorating group 7	12．74±3．60	1．40+1．49	4．29+1．37
				Normal group 4	l 8．30±1．88	3．25±7．03	7．03±O．65

Annotate: P＜O.05.

(3) to the influence of gonadal hormone: each organizes after the administration 45 days clear testosterone of extracting vein blood (T) respectively, estradiol (E2).The result: the kidney invigorating group serum T and E2 content increase.But no statistical significance (the P value is all＞0.05).

(4) to the influence of periphery physiochemical indice: administration 60 days and drug withdrawal are measured respectively in the time of 60 days and are respectively organized cock periphery hemoglobin, reticulocyte, and red, leukocyte count, testing result sees Table 12, table 13.

Above results suggest: why antiaging agent oral liquid the kidney invigorating effect can improve hemoglobin content may be relevant with its hemopoietic function improvement.

Conclusion

The traditional Chinese medical science is thought people's life, length, strong, old and kidney is closely related, the people mostly is physiological " kidney " void to old age, the existing insufficiency of kidney-YANG of male's multilist, sexual disorder, the women then mostly is the ischemia of suffering from a deficiency of the kidney, and endocrine dysfunction makes each hypofunction of body, presents aging phenomenon, the kidney being the origin of congenital constitution, the root of life.Therefore utilize the medicine of benefiting essence and marrow, YIN nourishing, tonifying YANG, the antiaging agent oral liquid is that specific action is arranged.．

Selecting the castrating cock is model, the obvious atrophy of cockscomb after the cock castration, and feather is loose matt, and behavior is blunt, and outward appearance is obviously feminized, and presents " suffering from a deficiency of the kidney " ischemic state.The cockscomb growth is one of outstanding feature of cock sex character growth.Therefore, from the sex character variation of formalness, can intuitively reflect the partial action of medicine.Experiment shows: the initial stage of taking medicine is fast with the growth of (the kidney invigorating group) cockscomb of kidney invigorating and YANG supporting, and is tall and big upright, plump, scarlet, with the castrating matched group very notable difference arranged, again administration after 60 days above-mentioned situation more obvious.60 days contrast difference are not remarkable after drug withdrawal.

From the peripheral hemogram analysis result: the kidney invigorating group all has and increases.After the drug withdrawal 60 days, the kidney invigorating group peripheral hemogram was still kept higher level.Kidney invigorating and YANG supporting is described, antiaging agent oral liquid medicine is lasting, and the after effect time is long.Prompting has the castrating of promotion cock sex character to grow and hematosis, so adopt benefiting essence and marrow, the kidney invigorating, tonifying YANG, more is tending towards " effecting a permanent cure ".Hormonal readiness is measured and is shown, the kidney invigorating, tonifying YANG, and the modulability hormonal action promotes the sex character developmental effects.

In sum, this medical instrument has benefiting essence and marrow and grows effects such as sun, tonifying YANG.The energy sex hormone level regulation promotes sexual function effect and function in delaying senility.

Four, the acute toxicity test of antiaging agent oral liquid

1, materials and methods: get 20 of the health large ear rabbits of 1.6-2.2kg, male and female half and half are divided into four groups at random.

The sorting table of 20 animals of table 14 during by four groups of random assortment

Numbering	1	2	3	4	5	6	7	8	9	10	11	12	13	14	15	16	17	18	19	20
																					Random digit	26	08	73	37	32	04	05	69	30	16	09	05	88	69	58	28	99	93	22	53
Remove remainder with 4	2	0	1	1	0	0	1	1	2	0	1	1	0	1	2	0	3	1	2	1
																					Distribute group	Two	Four	One	One	Four	Four	One	One	Two	Four	One	One	Four	One	Two	Four	Three	One	Two

Illustrate: remove each random number with group several 4, write down remainder.Pressing the big young pathbreaker animal of remainder divides to each group.Remainder is 1, is about to this animal and belongs to 1 group, and 2 return 2 groups, and 3 return 3 groups, and 0 returns four groups.

The grouping of table 15 completely random

One group	3 4 7 8 11 12 14 18 20
		Two groups	1 9 15 19
Three groups	17
		Four groups	2 15 6 10 13 16

Annotate: each treated animal is unequal, gets 4 numbers and remove first number with first group 9 earlier on random table, and 8 remove second number, and 7 remove the 3rd number, and 6 remove the 4th number.The gained remainder be respectively 5,5,3,4 arrange in order in being about to first group the 5th, and the like.

20 animal completely randoms of table 16 distribution result

Group	Number of animals
						First group	3	4	14	18	20
Second group	1	9	15	19	6
						The 3rd group	17	ll	12	7	8
The 4th group	2	15	10	13	16

Annotate: grouping back animal difference, sub-cage rearing

4 groups of white rabbits are taken out the antiaging agent oral liquid that gavages 4 kinds of various dose behind the stomach tube with syringe respectively.First group of 5ml contains 59: the second groups of 10ml of crude drug and contains 109: the three groups of 15ml of crude drug and contain 159: the four groups of 20ml of crude drug and contain crude drug 209.

The acute filling stomach contamination of table 17 log room temperature on August 10th, 1996

Medicine: antiaging agent oral liquid proportion: 5.5＜extracting solution 〉

Animal varieties: large ear rabbit dosage 20ml contains crude drug 209

Numbering	Body weight	Dosage	Toxic action	Death time
																		<g>	20ml	Performance	1 day	2	3	4	5	6	7	8	9	20	11	12	13	14
	2	1、6	、	0	0	0	0	0	0	0	0	0	0	0	0	0	0																		0
15																		2、2	、	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	10	2、2	、	0	0	0	0	0	0	0	0	0	0	0	0	0	0																		0
13																		1、6	、	0	0	0	0	0	0	0	0	0	0	0	0	0	0	0
	16	1、6	、	0	0	0	0	0	0	0	0	0	0	0	0	0	0																		0

2, result:

After (1) four group of white rabbit gavages the antiaging agent oral liquid of 4 kinds of various dose respectively, do not have dead.None lists the existing psychological problem and the phenomena of mortality as a result after gavaging 14 days continuously, and the white rabbit weight increase is more active on the contrary.See more satin of white rabbit fur with appearance.

(2) analyze: this medicine avirulence medical material, no eighteen incompatible medicaments nineteen medicaments of mutual restraint incompatibility.

Its effect of antiaging agent oral liquid is: benefiting essence and marrow, help intensive culture god, YIN nourishing, tonifying YANG and heat-clearing and toxic substances removing.Therefore it is spiritual more active that White Rabbit is irritated drink more.

Five, check, probation report list

1, table 18 antiaging agent oral liquid hygiene inspection report

Solution colour	Pale brown color
		Transparency	Transparent
The sense of taste	Sweet, little hardship
		PH value	5．5
Proportion	1．15
		Through centrifugal 3000/ commentaries on classics/10 '	There is not precipitation
96 years JIUYUE of trier Wang Wei China 20 days

2, table 19 antiaging agent oral liquid count of bacteria report as a result

Average bacterium colony numerical example	Test sample dilution figure place			The ratio of two dilution factor bacterium numbers	Total plate count T/ml	Total number of bacteria
							10-1	10-2	10-3
	1									-	-	＜10
2								-	-				＜10
	3									-	-	＜10
4								-	-				＜10
	Remarks: the equal asepsis growth of each extension rate

Every batch of test sample extracts number: every bottle of 250ml, totally 2 bottles.Trier: Wang Weihua.

3, table 20 antiaging agent oral liquid colonial morphology and probation report list

Serum	A--F is many. valency 0 serum		The normal saline contrast	General biochemical reaction	Report
						Bacterium to be checked	Lawn	100° 0.3＇
In proper order
	1	-				-			-	-	Do not detect Salmonella
Trier Wang Wei China JIUYUE in 1996 20 days

4, table 21 Salmonella testing procedure diagram

5, antiaging agent oral liquid chemical constituent inspection report

(1) glycoside inspection:

Method: get test liquid 1ml, drip a-2 joltings of naphthol reagent, drip dense stream acid, make to be divided into two liquid layers along test tube wall.

The result: purple-purplish red colour circle appears in two liquid layer intersections.(genus positive reaction)

(2) inspection of flavonoid glycoside:

Method: test liquid is directly used dissolve with ethanol (adding dissolve with ethanol if aqueous solution should concentrate) 2ml dropping concentrated hydrochloric acid, and (this moment, color reddened, illustrate and contain anthocyanidin) add then the magnesium powder granule a little, this moment in vitro promptly produce a large amount of hydrogen, bubble, warm a moment on the pond.

The result: it is red that purplish red-cherry appears in solution.(being positive reaction)

(3) inspection of Saponin:

Method: after the dilution of test liquid 1ml adding distil water, fierce jolting in test tube.

Result: produce a large amount of persistency foams and maintain more than 10 minutes more than 1/3 of foam volume, liquid volume.(being positive reaction)

(4) aminoacid, protein, polypeptide inspection:

Method: test liquid 1m1 adds ninhydrin test solution number droplet, heated and boiled 4-5 minutes.

Result: show redness-purple-brown-blue color.(being positive reaction)

6, the antiaging agent oral liquid is to the influence (resisting fatigue experiment) of rabbit swimming time

Equipment: get that high 60cm is wide, 2 of 2 of 40cm glass jars, 20ml syringe 2 tools, stomach tube, conduit, 2 of animal fixed mounts, 1 of animal scale, 2 of gags.

Medicine: antiaging agent oral liquid, reference substance 0.9% normal saline.

Animal: 20 of three months little rabbit, health, male and female half and half, by same forage feed, seven day 6ml after test matched group from a mouthful administration every day: normal saline 6ml.

Method: glass jar adds water, and depth of water 50cm, water temperature remain on 20 ± 0.5 ℃, get 20 rabbits, and female, hero half and half is weighed respectively, numbered, and is divided into two groups by the body weight sex.Respectively organize rabbit fasting 4 hours before being tried, respectively rabbit is fixed on the frame, insert conduit the conduit collar extension is placed a bottle that is filled with water, prove that as gassing not this pipe is in esophagus, draw with syringe then and tried thing, inject esophagus from conduit.Give antiaging agent oral liquid group 6ml/kg, matched group 6ml/kg normal saline, timing immediately after the injection, inject rabbit to be put in the glass jar respectively in back 30 minutes and swim, and reclocking, put into each rabbit at every turn, observe rabbit head submerged and can not the person of emerging be body weight exhaustion 10 seconds; At once timing is that swimming time and the matched group of rabbit carries out statistical procedures, and the antiaging agent oral liquid is to the influence of three months rabbit swimming times, experimental record:

Table 22

Group	Dosage ml/kg	Rabbit swimming time min
			Normal saline	6ml	12．00±2．10
The antiaging agent oral liquid	6ml	21．96±3．42

According to experimental result: the antiaging agent oral liquid has antifatigue effect.

Six, antiaging agent stability of Oral preliminary study

Adopt the constant temperature accelerated test, to the research that experimentizes of the stability of defying age medicine oral liquid.Experimental data is learned by statistics and is handled and graphing method is asked calculation, under 25 ℃ of conditions of room temperature, with 2 years effect duration of emodin standard.

The difference of various medicines on chemically stable is very big, the compound Chinese medicinal preparation complicated component, thereby the preliminary assay of effect duration is difficult.Present stage with a certain or several active component (preferably effective ingredient), as the preparation index of effect duration, be feasible.This paper adopts the constant temperature accelerated test method, through prerun, selects 55,65,75,90 ℃ for use, to main component emodin stability prediction in the defying age medicine oral liquid, simultaneously the variation of pH value in the degradation process is investigated.

1, assay method: (1) sample treatment: sample is close to be filled in centrifuge tube, places 55,65,75,90 ℃ of waters bath with thermostatic control respectively, timing sampling, and cooling, standby.(2) the former state trap is measured: sample thief 40ml adds methanol 50ml, puts in the apparatus,Soxhlet's, extracts, and waves most methanol, add 2.5mol/L sulphuric acid 15ml, chloroform 15ml, hydrolysis 2 hours, be transferred to after the cooling in the separatory funnel, divide and get chloroform layer, reuse 15ml chloroform extraction once.Combined chloroform liquid, (2 * 20ml) are washed till neutrality to distilled water.Water-bath boils off chloroform, and it is a small amount of to add 0.5% magnesium acetate methanol solution, transfers to after the dissolving in the 50ml volumetric flask, is diluted to scale.With 0.5% magnesium acetate methanol reagent is blank, decides absorption value with 721 spectrophotometers in the 498nm wave length calibration.(3) instant absorption of sample degree is measured: method is with above-mentioned former state absorption measurement.(4) relative amount is asked calculation: the relative amount of each time (%)=trap * 100% when instant absorption of sample degree/former state is zero.

2, experimental result:

(1) determination data: see Table 23

(2) determine the order of reaction with diagram method,,, be shown as straight line, illustrate that its change procedure meets pseudo first-order reaction with 1gc-t mapping according to heat time heating time and the changes of contents under each constant temperature of table 23.

(3) calculating K value: carry out linear regression according to different heating time under each constant temperature of table 23 and the logarithmic data of relative concentration, try to achieve the slope of each 1 line, and then try to achieve respective reaction rate constant K.The results are shown in Table 24.

Determination data under the different constant temperatures of table 23

Temperature ℃	Sample time (d)	0	8	18	28	38	46
								55	Measure absorption value	0．631	0．614	0．590	0．569	0．550	0．539
Relative concentration C (%)	100	97．32	93．50	90．17	87．16	85．49
							IgC		2．000	1．9882	1．9708	1．9551	1．9403	1．9319
65	Measure absorption value	0．631	0．602	0．583	0．561	0．510									0．466
							Relative concentration C (%)	100	95．40	92．39	88．91	80．82	73．85
	IgC	2．000	1．9795	1．9656	1．9490	1．9075								1．8684
							75	Measure absorption value	0．631	0．582	0．540	0．486	0．412		0．392
Relative concentration C (%)	100	92．23	85．58	77．02	65．29	62．12
								IgC	2．000	1．9649	1．9324	1．8866	1．8148	1．7932
90	Measure absorption value	0．631	0．525	0．42	0．341	0．268									0．229
							Relative concentration C (%)	100	83．20	66．72	54．08	42．47	36．29
	IgC	2．000	1．9201	1．8243	1．7330	1．6281								1．5593

K value when (4) asking room temperature (25 ℃) is handled relevant data in the table 24, the results are shown in Table 25.With 1gk 1/T is mapped, try to achieve room temperature (1/c273+25)=3.3557 * 10 ^-3The time K=5.053 * 10 ^-4).

Measure equation of linear regression and K value under each condition of table 24

Temperature ℃	Regression equation	r	Straight slope	The K value
					55	Igc=1．9993—0．0015169t	0．9981	—0．0015169	3．4934×10 —3
65	Igc=2．00685—0．0026892t	—9．9719	—0．0026892	6．1933×10 —3
					75	Igc=2．00548—0．0046450t	—0．9929	—0．0046450	1．0697×10 —2
90	Igc=1．9984—0．0096028t	—0．9998	—0．0096028	2．2115×10 —2

Table 25 temperature and rate constant logarithm synopsis

Temperature ℃	1／T	IgK
			55	3．0488×10 —3	—2．4568
65	2．9586×10 —3	—2．2081
			75	2．8736×10 —3	—1．9707
90	2．7548×10 —3	—1．6553

(5) 25 ℃ of effect duration (t0.9) ask calculation, be 737 days.

(6) mensurationization of pH value in the degradation process.See Table 26 (utilizing TOAHM-20E type acidometer)

PH value changes in table 26 degradation process

55℃		65℃		75℃		90℃
								Sample time h	PH value	Sample time h	PH value	Sample time h	PH value	Sample time h	PH value
0	5．5	0	5．5	0	5．5	0	5．5
								4	5．4	6	5．4	3	5．4	2	5．3
8	5．3	12	5．3	6	5．3	4	5．2
								12	5．0	18	5．2	10	5．15	6	5．1
24	4．7	24	5．0	16	5．1	8	5．0
								48	4．5	36	4．7	24	5．0	12	4．95
72	4．3	48	4．6	36	4．8	16	4．9
								144	4．6	72	4．5	48	4．6	24	4．8
360	4．8	120	4．8	72	4．8	36	4．8

PH value is more stable in its degradation process of antiaging agent oral liquid, and slight decline is arranged, and acidic materials have been described.

Conclusion

1, experiment shows after the big procatarxis temperature rise in the antiaging agent oral liquid downward trend is arranged, and its effect duration is 737 days.

2, pH value changes little in degradation process.

3, the antiaging agent oral liquid is a compound preparation, and its mechanism of action is very complicated, so the compound preparation effect duration prediction of Chinese medicine is difficult.Have only the effective ingredient that utilizes medicine to carry out prerun.

Above-mentioned all kinds of test by Wusu City, Xinjiang Ruan of institute of traditional Chinese medicine can be, Dai Liman, Zhou Xudong, the Li Aiping of Yi Li state, Xinjiang Kuitun hospital finish.Clinical observation for slow down aging, treatment insufficiency of kidney-YANG is finished by Ruan Kecai, Li Yuzhi, Dai Liman, Hou Dongxia, Wang Weihua, Yang Hongcheng, Ma Yongyao, the Xu Xinwu of Wusu City, Xinjiang hospital, Zhou Xudong, Zhao Wenkun, the Li Aiping of Yi Li state, Xinjiang Kuitun hospital, the Zhu Weiping of Kuytun City hospital, Dong Zhiyi, Zou Sulan etc. participate in and finish.

Embodiments of the invention 1: 25 parts in auricle, 15 parts of Radix Ginsengs, 10 parts of Hippocampus, 30 parts of Herba Epimedii, 35 parts of Fructus Psoraleaes, 30 parts of Radix Scutellariaes, 30 parts of Rhizoma Polygonatis, 30 parts of Radix Et Caulis Acanthopanacis Senticosis on 30 parts of calf thymuss, 20 parts of calf spleens, the pig heart; 30 parts of Radix Rehmanniae, 30 parts of Fructus Lycii, 25 parts of Radix Asparagi, 30 parts of the Radixs Astragali, 25 parts of the Rhizoma Atractylodis Macrocephalaes, 30 parts in Radix Glycyrrhizae, 30 parts of Radix Polygoni Multiflori; 30 parts of the Radix Paeoniae Albas, 30 parts of Radix Codonopsis, 30 parts of Placenta Hominiss, 30 parts of Herba Cistanches, 30 parts of Radix Isatidiss, 0.05 part of procaine, 30 parts of Fructus Ligustri Lucidi, 30 parts of Fructus Schisandrae Chinensis.

Embodiment 2: 3 parts in auricle, 3 parts of Radix Ginsengs, hippocampus 3 part, 10 parts of Herba Epimedii, 10 parts of Fructus Psoraleaes, 10 parts of Radix Scutellariaes, 10 parts of Rhizoma Polygonatis, 10 parts of Radix Et Caulis Acanthopanacis Senticosis on 8 parts of calf thymuss, 3 parts of calf spleens, the pig heart; 10 parts of Radix Rehmanniae, 10 parts of Fructus Lycii, 10 parts of Radix Asparagi, 10 parts of the Radixs Astragali, 8 parts of the Rhizoma Atractylodis Macrocephalaes, 8 parts in Radix Glycyrrhizae, 8 parts of Radix Polygoni Multiflori, 8 parts of the Radix Paeoniae Albas, 8 parts of Radix Codonopsis, 6 parts of Placenta Hominiss, 8 parts of Herba Cistanches, 8 parts of Radix Isatidiss, 0.0003 part of procaine, 8 parts of Fructus Ligustri Lucidi, 8 parts of Fructus Schisandrae Chinensis.

Embodiment 3: 15 parts in auricle, 15 parts of Radix Ginsengs, 6 parts of Hippocampus, 30 parts of Herba Epimedii, 25 parts of Fructus Psoraleaes, 25 parts of Radix Scutellariaes, 25 parts of Rhizoma Polygonatis, 25 parts of Radix Et Caulis Acanthopanacis Senticosis on 30 parts of calf thymuss, 15 parts of calf spleens, the pig heart; 15 parts of Radix Rehmanniae, 20 parts of Fructus Lycii, 15 parts of Radix Asparagi, 20 parts of the Radixs Astragali, 15 parts of the Rhizoma Atractylodis Macrocephalaes, 20 parts in Radix Glycyrrhizae, 20 parts of Radix Polygoni Multiflori; 15 parts of the Radix Paeoniae Albas, 25 parts of Radix Codonopsis, 25 parts of Placenta Hominiss, 20 parts of Herba Cistanches, 20 parts of Radix Isatidiss, 0.0006 part of procaine, 20 parts of Fructus Ligustri Lucidi, 20 parts of Fructus Schisandrae Chinensis.

Embodiment 4: the manufacture method of antiaging agent oral liquid Chinese medicine is: selected first-class medical material clean → was pulverized 40 mesh sieves, was weighed: 3 parts of Radix Ginsengs, hippocampus 3 part, 10 parts of Herba Epimedii, 10 parts of Fructus Psoraleaes, 10 parts of Radix Scutellariaes, 10 parts of Rhizoma Polygonatis, 10 parts of Radix Et Caulis Acanthopanacis Senticosis; 10 parts of Radix Rehmanniae, 10 parts of Fructus Lycii, 10 parts of Radix Asparagi, 10 parts of the Radixs Astragali, 8 parts of the Rhizoma Atractylodis Macrocephalaes, 8 parts in Radix Glycyrrhizae, 8 parts of Radix Polygoni Multiflori, 8 parts of the Radix Paeoniae Albas, 8 parts of Radix Codonopsis, 6 parts of Placenta Hominiss, 8 parts of Herba Cistanches, 8 parts of Radix Isatidiss, 8 parts of Fructus Ligustri Lucidi, 8 parts of Fructus Schisandrae Chinensis, merceration bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 deal by every milliliter and add distilled water, quiet straight 24 hours → vacuum filtration → quiet straight 24 hours → vacuum filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.

The preparation method of the animal drugs of joining with the foregoing description 4 medicines is: with the calf thymus of getting ready, the calf spleen, auricle is in advance at-8 ~-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → slurrying → take by weighing with distilled water on the pig heart: 8 parts of calf thymuss, 3 parts of calf spleens, saline leaching in the life of 3 parts → adding of auricle doubling dose on the pig heart, soaked 1 hour at room temperature earlier, again 75 ~ 80 ℃ soak filtered in 0.5 hour soak again 2 ~ 3 times → with the rapid heated and boiled of filtrate 3 ~ 5 minutes, making medicinal liquid slightly be chilled to 40 ~ 60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, it is 1.15 that 0.0003 ~ 0.05 part of adapted of procaine is surveyed proportion, check → dress envelope → sterilization → check → lettering → packing.

Embodiment 5: the manufacture method of antiaging agent oral liquid Chinese medicine is: selected first-class medical material clean → was pulverized 40 mesh sieves, was weighed: 15 parts of Radix Ginsengs, 10 parts of Hippocampus, 30 parts of Herba Epimedii, 35 parts of Fructus Psoraleaes, 30 parts of Radix Scutellariaes, 30 parts of Rhizoma Polygonatis, 30 parts of Radix Et Caulis Acanthopanacis Senticosis; 30 parts of Radix Rehmanniae, 30 parts of Fructus Lycii, 25 parts of Radix Asparagi, 30 parts of the Radixs Astragali, 25 parts of the Rhizoma Atractylodis Macrocephalaes, 30 parts in Radix Glycyrrhizae, 30 parts of Radix Polygoni Multiflori; 30 parts of the Radix Paeoniae Albas, 30 parts of Radix Codonopsis, 30 parts of Placenta Hominiss, 30 parts of Herba Cistanches, 30 parts of Radix Isatidiss, 30 parts of Fructus Ligustri Lucidi, 30 parts of Fructus Schisandrae Chinensis, merceration bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 deal by every milliliter and add the quiet straight 24 hours → vacuum filtration of distilled water → quiet straight 24 hours → vacuum filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.

The preparation method of the animal drugs of joining with the foregoing description 5 medicines is: with the calf thymus of getting ready, the calf spleen, auricle is in advance at-8 ~-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → slurrying → take by weighing with distilled water on the pig heart: 30 parts of calf thymuss, 20 parts of calf spleens, saline leaching in the life of 25 parts → adding of auricle doubling dose on the pig heart, soaked 1 hour at room temperature earlier, again 75 ~ 80 ℃ soak filtered in 0.5 hour soak again 2 ~ 3 times → with the rapid heated and boiled of filtrate 3 ~ 5 minutes, making medicinal liquid slightly be chilled to 40 ~ 60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, the Pu Lu point is because of 0.0003 ~ 0.05 part of adapted, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.

Embodiment 6: antiaging agent oral liquid preparation method of Chinese medicine method is: selected first-class medical material clean → was pulverized 40 mesh sieves, title: 15 parts of Radix Ginsengs, 6 parts of Hippocampus, 30 parts of Herba Epimedii, 25 parts of Fructus Psoraleaes, 25 parts of Radix Scutellariaes, 25 parts of Rhizoma Polygonatis, 25 parts of Radix Et Caulis Acanthopanacis Senticosis; 15 parts of Radix Rehmanniae, 20 parts of Fructus Lycii, 15 parts of Radix Asparagi, 20 parts of the Radixs Astragali, 15 parts of the Rhizoma Atractylodis Macrocephalaes, 20 parts in Radix Glycyrrhizae, 20 parts of Radix Polygoni Multiflori; 15 parts of the Radix Paeoniae Albas, 25 parts of Radix Codonopsis, 25 parts of Placenta Hominiss, 20 parts of Herba Cistanches, 20 parts of Radix Isatidiss, 20 parts of Fructus Ligustri Lucidi, 20 parts of Fructus Schisandrae Chinensis, merceration bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 deal by every milliliter and add distilled water, after quiet straight 24 hours → vacuum filtration → quiet straight 24 hours final vacuum sucking filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join.

The preparation method of the animal drugs of joining with the foregoing description 6 medicines is: with the calf thymus of getting ready, the calf spleen, auricle is in advance at-8 ~-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → slurrying → take by weighing with distilled water on the pig heart: 30 parts of calf thymuss, 20 parts of calf spleens, the normal saline of 25 parts → adding of auricle doubling dose leaches on the pig heart, soaked 1 hour at room temperature earlier, again 75 ~ 80 ℃ soak filtered in 0.5 hour soak again 2 ~ 3 times → with the rapid heated and boiled of filtrate 3 ~ 5 minutes, making medicinal liquid slightly be chilled to 40 ~ 60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively to get final product, with Chinese medicine, animal drugs, 0.0003 ~ 0.05 part of adapted of procaine, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.

Claims (3)

1, a kind of antiaging agent oral liquid is characterized in that said composition is to make 8 ~ 30 parts of calf thymuss by the following raw materials by weight proportions thing, 3 ~ 20 parts of calf spleens, auricle is 3 ~ 25 parts on the pig heart, 3 ~ 15 parts of Radix Ginsengs, hippocampus 3 ~ 10 part, 10 ~ 30 parts of Herba Epimedii, 10 ~ 35 parts of Fructus Psoraleaes, 10 ~ 30 parts of Radix Scutellariaes, 10 ~ 30 parts of Rhizoma Polygonatis, 10 ~ 30 parts of Radix Et Caulis Acanthopanacis Senticosis, 10 ~ 30 parts of Radix Rehmanniae, 10 ~ 30 parts of Fructus Lycii, 10 ~ 25 parts of Radix Asparagi, 10 ~ 30 parts of the Radixs Astragali, 8 ~ 25 parts of the Rhizoma Atractylodis Macrocephalaes, 8 ~ 30 parts in Radix Glycyrrhizae, 8 ~ 30 parts of Radix Polygoni Multiflori, 8 ~ 30 parts of the Radix Paeoniae Albas, 8 ~ 30 parts of Radix Codonopsis, 6 ~ 30 parts of Placenta Hominiss, 8 ~ 30 parts of Herba Cistanches, 8 ~ 30 parts of Radix Isatidiss, 0.0003 ~ 0.05 part of procaine, 8 ~ 30 parts of Fructus Ligustri Lucidi, 8 ~ 30 parts of Fructus Schisandrae Chinensis.

2, antiaging agent oral liquid according to claim 1 is characterized in that material medicine optimum weight proportioning is 30 parts of calf thymuss, 15 parts of calf spleens, auricle is 15 parts on the pig heart, 15 parts of Radix Ginsengs, 6 parts of Hippocampus, 30 parts of Herba Epimedii, 25 parts of Fructus Psoraleaes, 25 parts of Radix Scutellariaes, 25 parts of Rhizoma Polygonatis, 25 parts of Radix Et Caulis Acanthopanacis Senticosis, 15 parts of Radix Rehmanniae, 20 parts of Fructus Lycii, 15 parts of Radix Asparagi, 20 parts of the Radixs Astragali, 15 parts of the Rhizoma Atractylodis Macrocephalaes, 20 parts in Radix Glycyrrhizae, 20 parts of Radix Polygoni Multiflori, 15 parts of the Radix Paeoniae Albas, 25 parts of Radix Codonopsis, 25 parts of Placenta Hominiss, 20 parts of Herba Cistanches, 20 parts of Radix Isatidiss, 0.0006 part of procaine, 20 parts of Fructus Ligustri Lucidi, 20 parts of Fructus Schisandrae Chinensis.

3, a kind of preparation method of claim 1 or 2 medicine oral liquid, it is characterized in that selected first-class medical material clean → pulverized 40 mesh sieves, take by weighing 3 ~ 15 parts of Radix Ginsengs, hippocampus 3 ~ 10 part, 10 ~ 30 parts of Herba Epimedii, 10 ~ 35 parts of Fructus Psoraleaes, 10 ~ 30 parts of Radix Scutellariaes, 10 ~ 30 parts of Rhizoma Polygonatis, 10 ~ 30 parts of Radix Et Caulis Acanthopanacis Senticosis, 10 ~ 30 parts of Radix Rehmanniae, 10 ~ 30 parts of Fructus Lycii, 10 ~ 25 parts of Radix Asparagi, 10 ~ 30 parts of the Radixs Astragali, 8 ~ 25 parts of the Rhizoma Atractylodis Macrocephalaes, 8 ~ 30 parts in Radix Glycyrrhizae, 8 ~ 30 parts of Radix Polygoni Multiflori, 8 ~ 30 parts of the Radix Paeoniae Albas, 8 ~ 30 parts of Radix Codonopsis, 6 ~ 30 parts of Placenta Hominiss, 8 ~ 30 parts of Herba Cistanches, 8 ~ 30 parts of Radix Isatidiss, 8 ~ 30 parts of Fructus Ligustri Lucidi, 8 ~ 30 parts of mercerations of Fructus Schisandrae Chinensis bubble 24 hours → put into multi-function extractor water to carry 2 times, 1.5 hours for the first time, for the second time 1/5 → precipitate with ethanol of 2 hours merging filtrate → vacuum concentration original volumes makes medicinal liquid contain amount of alcohol to reach 75% back and place 24 hours → vacuum filtration → vacuum and reclaim ethanol → vacuum decompression and concentrate → contain crude drug in whole 1 deal by every milliliter and add distilled water after quiet straight 24 hours → vacuum filtration → quiet straight 24 hours final vacuum sucking filtration, surveying proportion should be 1.15 → transfer PH be 5.5 → check → wait and join; With the calf thymus of getting ready, the calf spleen, auricle is in advance at-8～-20 ℃ of stored frozen one week → remove connective tissue and impurity → clean → 8～30 parts of calf thymuss of slurrying → take by weighing with distilled water on the pig heart, 3～20 parts of calf spleens, the normal saline of 3～25 parts → adding of auricle doubling dose leaches on the pig heart, earlier room temperature soak 1 hour again 75～80 ℃ soak filtered in 0.5 hour soak again 2～3 times → with the rapid heated and boiled of filtrate 3～5 minutes, making medicinal liquid slightly be chilled to 40 ~ 60 ℃ uses 6.804 kilograms of pressure through 30 minutes heat treated with paper pulp cake sucking filtration → filtrate with the medicinal liquid that sodium hydroxid transfers to PH7.2 → will control out immediately, cool off with frozen water then, reuse paper pulp cake filters, filtrate transfers to PH7.2 again, 6.804 kilograms of pressure of reuse were through heat treatment 1 hour immediately, cold preservation filtering in 24 hours, bottling is with 6.804 kilograms of pressure pressure sterilizings, check transparency, be no more than 8% as percent defective and get final product adapted, said medicine is transferred to PH5.5 respectively can be with Chinese medicine, animal drugs, 0.0003 ~ 0.05 part of adapted of procaine, surveying proportion is 1.15, check → dress envelope → sterilization → check → lettering → packing.”