CN117168940A - Slide loading device of immunohistochemical analyzer - Google Patents
Slide loading device of immunohistochemical analyzer Download PDFInfo
- Publication number
- CN117168940A CN117168940A CN202311124396.6A CN202311124396A CN117168940A CN 117168940 A CN117168940 A CN 117168940A CN 202311124396 A CN202311124396 A CN 202311124396A CN 117168940 A CN117168940 A CN 117168940A
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- cover plate
- plate
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- liquid
- slide
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- 230000002055 immunohistochemical effect Effects 0.000 title claims description 15
- 239000007788 liquid Substances 0.000 claims abstract description 121
- 239000011521 glass Substances 0.000 claims abstract description 68
- 238000002991 immunohistochemical analysis Methods 0.000 claims abstract description 3
- 238000005070 sampling Methods 0.000 claims abstract description 3
- 238000000926 separation method Methods 0.000 claims description 32
- 239000002699 waste material Substances 0.000 claims description 30
- 239000003153 chemical reaction reagent Substances 0.000 claims description 26
- 238000007599 discharging Methods 0.000 claims description 22
- 238000009423 ventilation Methods 0.000 claims description 6
- 239000000523 sample Substances 0.000 description 33
- 238000010438 heat treatment Methods 0.000 description 12
- 238000005452 bending Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 6
- 239000012472 biological sample Substances 0.000 description 4
- 230000005540 biological transmission Effects 0.000 description 4
- 238000013519 translation Methods 0.000 description 4
- 238000001816 cooling Methods 0.000 description 3
- 238000004043 dyeing Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000011532 immunohistochemical staining Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 239000013039 cover film Substances 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000003364 immunohistochemistry Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000010827 pathological analysis Methods 0.000 description 1
Abstract
The utility model provides an immunohistochemical analysis appearance slide loading device, including the slide glass of carrying platform, the cover plate, actuating mechanism, cover plate mechanism, flowing back mechanism, remove bubble mechanism and sampling mechanism, place the slide glass that contains the sample on the slide glass, place the cover plate above the slide glass, fix the sample on the slide glass through the cover plate, cover plate mechanism is installed on the slide glass through the direction subassembly liftable, cover plate mechanism is used for pressing together the cover plate on the slide glass, actuating mechanism is connected with cover plate mechanism, flowing back mechanism respectively, be connected with the relative slide glass of actuating mechanism cover plate mechanism and remove, in order to press together the cover plate on the slide glass, flowing back mechanism is connected with the cover plate, be equipped with the negative pressure chamber in the slide glass, be equipped with the negative pressure mouth on the negative pressure chamber, drive the cover plate through flowing back mechanism and remove towards the negative pressure mouth in the negative pressure chamber, so that the liquid between cover plate, the slide glass is discharged through the negative pressure mouth, jack up one side of cover plate through removing the bubble mechanism with the cover plate, so that the slide glass is inclined with the bubble.
Description
Technical Field
The invention belongs to the technical field of immunohistochemistry, and particularly relates to a slide loading device of a full-automatic immunohistochemical analyzer.
Background
When the full-automatic immunohistochemical staining instrument performs staining operation, a biological sample is required to be placed on a glass slide, a very thin reaction cavity is formed by utilizing the matching of a liquid cover film and the glass slide, and a reagent is added at one end of the reaction cavity so as to enable the biological sample to fully react with the reaction liquid, and the operations can be realized in the immunohistochemical staining instrument at present.
The invention patent application number CN202010843934.7 discloses a biological sample processing device comprising: a cam carrier; the heating module mounting seat is arranged above the cam rack; the heating modules are arranged on the heating module mounting seats; the rear side of each heating module is provided with a waste liquid collecting port, the rear part in the heating module mounting seat is provided with a waste liquid collecting cavity communicated with each waste liquid collecting port, and the bottom of the heating module mounting seat is provided with a waste liquid total outlet communicated with the waste liquid collecting cavity; the waste liquid collecting port, the waste liquid collecting cavity and the waste liquid main outlet form a liquid discharging system; a cooling air duct is arranged right below the heating module in the heating module mounting seat, the cooling air duct and the liquid discharging system are independent from each other, and a gas total inlet and a gas total outlet which are communicated with the cooling air duct are arranged on the heating module mounting seat. The biological sample processing equipment has the defects that (1) the liquid is discharged through an air suction mode, the liquid discharge efficiency of the liquid discharge mode is poor, the liquid discharge is incomplete, and the experimental effect of the subsequent reagent addition is affected; (2) When bubbles appear in front of the cover plate and the slide, no effective way of removing the bubbles exists, so that if the bubbles are on a tissue slice, local staining false negative can be caused, and normal diagnosis results are affected.
Disclosure of Invention
Aiming at the defects, the invention aims to provide the slide loading device of the immunohistochemical analyzer, which is used for improving the test accuracy and the operation convenience of the loading device.
In order to solve the technical problems, the invention adopts the technical proposal that,
an immunohistochemical analyzer slide loading device, comprising
The device comprises a carrying platform, a cover plate and a sample holder, wherein the carrying platform is provided with the cover plate, the cover plate is matched with the glass slide, a sample is fixed on the glass slide, a negative pressure cavity is arranged in the carrying platform, and a negative pressure port is arranged on the negative pressure cavity;
a driving mechanism;
the cover plate mechanism is connected with the driving mechanism, and drives the cover plate mechanism to move relative to the carrying platform through the driving mechanism so as to fix the cover plate on the carrying platform, and the reagent to be emptied on the carrying platform can be brought into the waste liquid port;
the liquid discharging mechanism is connected with the cover plate, and drives the liquid discharging mechanism to move through the driving mechanism so as to drive the cover plate to move towards the negative pressure port of the negative pressure cavity, so that liquid between the cover plate and the glass slide is discharged through the negative pressure port;
and the bubble removing mechanism is matched with the cover plate, and one side of the cover plate is jacked up through the bubble removing mechanism, so that the cover plate inclines relative to the glass slide to discharge bubbles.
As a preferable scheme of the invention, the carrying platform comprises a base and a negative pressure box body spliced on the base for forming a negative pressure cavity, a gas-liquid separation plate is arranged in the negative pressure box body, the gas-liquid separation plate is arranged below a negative pressure port, and an air channel is arranged on the gas-liquid separation plate.
As a preferable scheme of the invention, the gas-liquid separation plate is obliquely arranged on the negative pressure box body, the high side and the low side of the gas-liquid separation plate are respectively connected with the first guide plate and the second guide plate, the height of the second guide plate is higher than that of the first guide plate and the gas-liquid separation plate, the ventilation groove is arranged at the top of the second guide plate, and the gas-liquid separation plate is provided with a liquid outlet.
As a preferable scheme of the invention, baffle plates are connected to the inclined two sides of the first guide plate and the gas-liquid separation plate, the first guide plate, the gas-liquid separation plate and the baffle plates are encircled to form a liquid discharge cavity, and the liquid discharge port is arranged in the liquid discharge cavity.
As a preferable scheme of the invention, a glass slide table is arranged on the negative pressure box body, the glass slide table comprises a mounting base and a placing seat arranged on the mounting base, a waste discharge port is arranged on the placing seat, a waste discharge pipe is arranged on the mounting base, the waste discharge port is connected with the waste discharge pipe to form a negative pressure port, and the tail end position of the waste discharge pipe is lower than the ventilation groove.
As a preferable scheme of the invention, the liquid draining mechanism comprises a cover plate moving plate, the cover plate moving plate is inserted into the cover plate, the driving mechanism is connected with the cover plate moving plate, and the driving mechanism drives the cover plate moving plate to translate relative to the glass slide, so that liquid between the cover plate and the glass slide moves to the position above the negative pressure port along with the cover plate.
As a preferable scheme of the invention, the bubble removing mechanism comprises a bubble removing driver and a top plate, wherein the top plate is arranged on the carrying platform in a lifting manner, the bubble removing driver is connected with the top plate and drives the top plate to lift, the top plate is provided with a supporting plate, and the supporting plate is matched with the cover plate.
As a preferable scheme of the invention, the cover plate is provided with the slot, one side of the cover plate is in sliding connection with the liquid draining mechanism through the slot, and the supporting plate is propped against one side of the slot so as to jack up the cover plate through the supporting plate.
As a preferable mode of the present invention, the top plate is arranged below the liquid discharging mechanism, and the lifting height of the top plate is limited by the liquid discharging mechanism.
As a preferable scheme of the invention, the cover plate mechanism comprises a cover plate and a pressing frame, wherein the cover plate is arranged on the carrying platform in a lifting manner through the guide component, the pressing frame is arranged on the inner side end surface of the cover plate, and the cover plate is pressed through the pressing frame.
As a preferable scheme of the invention, the loading device further comprises a sample adding mechanism, wherein the sample adding mechanism comprises a sample adding driving piece and a sample adding head, the sample adding head is arranged on the sample adding driving piece, and the sample adding head is driven to lift by the sample adding driving piece.
The invention has the beneficial effects that (1) the liquid draining mechanism moves the cover plate to enable the cover plate and the glass slide to be misplaced, and due to the tension effect of the liquid, the liquid is carried away from the glass slide when the cover plate moves outside the glass slide, so that the reagent is conveniently drained from the space between the cover plate and the glass slide through the negative pressure in the negative pressure cavity, the reagent draining efficiency is improved, the reagent is prevented from remaining between the cover plate and the glass slide after the liquid draining operation, and the reagent is prevented from being diluted.
One side of the cover plate is jacked up through the bubble removing mechanism, so that the slide glass of the cover plate is inclined relatively, the reagent is gathered towards the overlapping direction of the slide glass and the cover plate, and when the cover plate is covered again, the reagent is distributed in the gap between the slide glass and the cover plate again, thereby playing the role of removing bubbles.
The sample adding mechanism can simultaneously add samples of the large-capacity auxiliary reagents at all positions, and compared with a mechanism with only one sample adding device, the sample adding time is obviously shortened, so that the dyeing period of the immunohistochemical dyeing instrument is shortened, and the waiting time of clinical pathological diagnosis is shortened.
By adopting two or more negative pressure connectors, the negative pressure in the cavity is uniform, and the negative pressure of the wild dispatching opening at each position can be ensured to be uniform, thereby ensuring the uniformity of dyeing.
Drawings
Fig. 1 is a schematic diagram of the structure of the device.
Fig. 2 is a schematic view of the structure after the cover plate mechanism is removed.
Fig. 3 is a partial enlarged view at a.
Fig. 4 is a schematic structural view of the lifting frame.
Fig. 5 is a schematic structural view of the cover plate mechanism.
Fig. 6 is a schematic structural view of the base.
Fig. 7 is a schematic structural view of the negative pressure cartridge.
Fig. 8 is a schematic view of the structure of fig. 7 rotated at a certain angle.
Fig. 9 is a schematic structural view of the gas-liquid separator.
Fig. 10 is a schematic structural view of the cover sheet.
Fig. 11 is a schematic structural view of the top plate.
Fig. 12 is a schematic structural view of the cover sheet moving plate.
Reference numerals: the device comprises a carrying platform 1, a negative pressure cavity 1-1, a negative pressure port 1-2, a base 1-3, a negative pressure box body 1-4, a gas-liquid separation plate 1-5, a ventilation groove 1-6, a first guide plate 1-7, a second guide plate 1-8, a liquid outlet 1-9, a baffle plate 1-10, a liquid outlet groove 1-11 and a negative pressure connector 1-12;
cover plate 2, slot 2-1, cover plate 2-2, connector 2-3;
the driving mechanism 3, the driving motor 3-1, the transmission shaft 3-2 and the cam 3-3;
the cover plate mechanism 4, the cover plate 4-1 and the pressing frame 4-2;
the liquid discharging mechanism 5, the cover plate moving plate 5-1, the driving bending plate 5-5, the translation roller 5-3, the sliding rail 5-4 and the driving bending plate 5-5;
the bubble removing mechanism 6, a bubble removing driver 6-1, a top plate 6-2, a supporting plate 6-3 and an upright post 6-4;
the glass slide table 7, the mounting base 7-1, the placing seat 7-2, the waste discharge port 7-3, the waste discharge pipe 7-4 and the limiting step 7-5;
the sample adding mechanism 8, the sample adding driving piece 8-1, the sample adding head 8-2 and the connecting piece 8-3;
the device comprises a guide assembly 9, a guide rod 9-1, a lifting frame 9-2, a spring 9-3 and a lifting roller 9-4.
Description of the embodiments
The invention is further described below with reference to the accompanying drawings.
The utility model provides an immunohistochemical analysis appearance slide loading device, including the platform 1, cover plate 2, actuating mechanism 3, apron mechanism 4, liquid discharging mechanism 5, remove bubble mechanism 6 and sampling mechanism 8, place the slide glass that contains the sample on the platform 1, place cover plate 2 above the slide glass, fix the sample on the slide glass through cover plate 2, apron mechanism 4 is installed on platform 1 through direction subassembly 9 liftable, cover plate mechanism 4 is used for pressing cover plate 2 on the slide glass, actuating mechanism 3 is connected with cover plate mechanism 4, liquid discharging mechanism 5 respectively, drive cover plate mechanism 4 through actuating mechanism 3 and remove the relative platform 1 and remove bubble mechanism 5, be connected with cover plate 2 with the cover plate 2 on the platform 1, be equipped with negative pressure chamber 1-1 in platform 1, be equipped with negative pressure 1-2 on the negative pressure chamber 1-1, drive cover plate 2 towards negative pressure 1-2 of negative pressure chamber 1-1 through liquid discharging mechanism 5 negative pressure makes 2, liquid between the cover plate 2 through negative pressure chamber 1-1 makes the cover plate 2, remove bubble through the negative pressure chamber 2 that the mechanism that removes the cover plate 2 is relative to the cover plate 2, remove bubble with the side of cover plate 2 through the mechanism that inclines, remove the cover plate 2.
In this embodiment, the liquid draining mechanism 5 moves the cover plate 2, so that the cover plate 2 and the glass slide are dislocated, and due to the tension of the liquid, when the cover plate 2 moves out of the glass slide, the liquid is carried away from the glass slide, so that the reagent is conveniently drained from between the cover plate 2 and the glass slide through the negative pressure in the negative pressure cavity 1-1, the draining efficiency of the reagent is improved, and the reagent is prevented from remaining between the cover plate 2 and the glass slide after the liquid draining operation.
One side of the cover plate 2 is jacked up through the bubble removing mechanism 6, so that the glass slides of the cover plate 2 are inclined relatively, reagents are gathered towards the overlapping directions of the glass slides and the cover plate 2, and when the cover plate 2 is covered again, the reagents are distributed in gaps between the glass slides and the cover plate 2 again, so that the bubble removing effect is achieved.
The driving mechanism 3 comprises a driving motor 3-1 and a transmission shaft 3-2, the driving motor 3-1 is connected with the transmission shaft 3-2 through a worm and a worm wheel, the transmission direction of the driving motor 3-1 is changed through the worm and the worm wheel, the rotation shaft 3-2 is arranged on the carrying platform 1 through a bearing, two ends of the rotation shaft 3-2 extend out of the carrying platform 1, cams 3-3 are arranged at two side end parts of the rotation shaft 3-2, the cams 3-3 are respectively connected with the cover plate mechanism 4 and the liquid discharging mechanism 5, the cover plate mechanism 4 is driven to move up and down through rotation of the cams 3-3, and the cams 3-3 further rotate to drive the liquid discharging mechanism 5 to move back and forth relative to the carrying platform 1.
The carrying platform 1 comprises a base 1-3 and a negative pressure box body 1-4, the negative pressure box body 1-4 is assembled on the base 1-3, the base 1-3 and the negative pressure box body 1-4 are enclosed to form a negative pressure cavity 1-1, a gas-liquid separation piece is arranged in the negative pressure box body 1-4 and comprises a gas-liquid separation plate 1-5, a first guide plate 1-7, a second guide plate 1-8 and a baffle plate 1-10, the gas-liquid separation plate 1-5 is obliquely arranged relative to the base 1-3, a negative pressure port 1-2 is arranged towards the gas-liquid separation plate 1-5, the first guide plate 1-7 and the second guide plate 1-8 are respectively connected to the high side and the low side of the gas-liquid separation plate 1-5, so that the connection structures of the gas-liquid separation plate 1-5, the first guide plate 1-7 and the second guide plate 1-8 are N-shaped, the two inclined sides of the first guide plate 1-7 and the gas-liquid separation plate 1-5 are connected with baffle plates 1-10, the first guide plate 1-7, the gas-liquid separation plate 1-5 and the baffle plates 1-10 are encircled to form a liquid discharge cavity, the liquid discharge port 1-9 is arranged in the liquid discharge cavity, the liquid discharge port 1-9 is arranged at the lowest end of the gas-liquid separation plate 1-5 for facilitating the molding of the liquid discharge port 1-9, the height of the second guide plate 1-8 is higher than that of the first guide plate 1-7 and the gas-liquid separation plate 1-5, the second guide plate 1-8 is abutted against the inner top surface of the negative pressure box body 1-4, an air vent groove 1-6 is formed at the top of the second guide plate 1-8, and in the liquid discharge process, the air with waste liquid is sucked into the negative pressure cavity 1-1, the inertia of the liquid is larger, so that the liquid collides with the gas-liquid separation plate 1-5 and adheres to the gas-liquid separation plate 1-5, along with the increase of liquid particles, the liquid flows to the liquid outlet 1-9 along the gas-liquid separation plate 1-5 and is discharged out of the negative pressure cavity, and most of air flows to the other side of the second guide plate 1-8 through the ventilation groove 1-6 due to the fact that the exhaust fan is arranged at the other side of the second guide plate 1-8.
The negative pressure box body 1-4 is provided with a slide table 7, the slide table 7 comprises a mounting base 7-1 fixed on the negative pressure box body 1-4 and a placing seat 7-2 arranged on the mounting base 7-1, the placing seat 7-2 is provided with a waste discharge port 7-3, the mounting base 7-1 is provided with a waste discharge pipe 7-4, the waste discharge port 7-3 is connected with the waste discharge pipe 7-4 to form a negative pressure port 1-2, the tail end position of the waste discharge pipe 7-4 is lower than the vent groove 1-6, a heating device for indirectly heating a slide is arranged on the inner top surface of the negative pressure box body 1-4, and through the arrangement of the waste discharge pipe 7-4, liquid can be prevented from flowing to the heating device of the device through the vent groove 1-6, so that the service life of the device is ensured.
The upper end of the waste discharge port 7-3 is provided with a limiting step 7-5, the placing position of the glass slide is positioned through the limiting step 7-5, and the limiting step 7-5 can prevent the glass slide from moving along with the cover plate 2 in the moving process of the liquid discharge mechanism 5 to the cover plate 2, so that the using stability of the device is improved.
The base 1-3 is provided with a liquid discharge groove 1-11 and a plurality of negative pressure connectors 1-12, waste liquid is discharged out of the carrying platform 1 through the liquid discharge groove 1-11, and the negative pressure connectors 1-12 are used for being connected with an air draft device, so that negative pressure is formed in the negative pressure cavity 1-1.
The cover plate 2 comprises a cover plate 2-2 and a connector 2-3, the cover plate 2-2 is covered on a glass slide, the connector 2-3 is used for being connected with a liquid discharging mechanism 5 and a bubble removing mechanism 6 to finish liquid discharging and bubble removing operation, a slot 2-1 is arranged in the connector 2-3, the liquid discharging mechanism 5 is inserted in the slot 2-1, so that the cover plate 2 can conveniently move along with the liquid discharging mechanism 5, meanwhile, the slot 2-1 and the liquid discharging mechanism 5 are in sliding connection, namely, the liquid discharging mechanism 5 can vertically move in the slot 2-1, and the cover plate 2 can be conveniently jacked up through the bubble removing mechanism 6 in the bubble removing process.
The cover plate mechanism 4 comprises a cover plate 4-1 and a pressing frame 4-2, wherein the cover plate 4-1 is installed on the carrying platform 1 in a lifting mode through a guide assembly 9, the pressing frame 4-2 is arranged on the inner side end face of the cover plate 4-1, and when the cover plate 4-1 moves downwards, the cover plate 2 is pressed on a glass slide through the pressing frame 4-2.
The guide assembly 9 comprises a guide rod 9-1 and a lifting frame 9-2, the guide rod 9-1 is fixed on two sides of the carrying platform 1, the lifting frame 9-2 is slidably mounted on the guide rod 9-1, a spring 9-3 is arranged between the lifting frame 9-2 and the carrying platform 1, the lifting frame 9-2 is jacked up through the spring 9-3, two sides of the cover plate 4-1 are respectively connected with the lifting frame 9-2, lifting rollers 9-4 are arranged on the lifting frame 9-2, the lifting rollers 9-4 are matched with the cams 3-3, and when the cams 3-3 rotate, power is transmitted to the lifting frame 9-2 through the lifting rollers 9-4, so that the cover plate 4-1 is driven to lift.
The liquid draining mechanism 5 comprises a cover plate moving plate 5-1, driving bending plates 5-5 are formed on two sides of the cover plate moving plate 5-1 in a bending mode, a translation roller 5-3 is installed on a cam 3-3, after the cam 3-3 rotates to a certain position, the translation roller 5-3 is abutted with the driving bending plate 5-5, and accordingly the driving bending plate 5-5 is driven to move back and forth, the cover plate moving plate 5-1 is convenient to move back and forth, and due to the fact that the cover plate 2 is connected with the liquid draining mechanism 5, when the cover plate moving plate 5-1 moves in a translation mode, the cover plate 2 also moves along with the cover plate moving plate 5-1, and therefore liquid between the cover plate 2 and a glass slide moves to the position above the negative pressure opening 1-2 along with the cover plate under the action of liquid tension.
The middle part of the cover plate moving plate 5-1 is bent to form a plugboard 5-3, the top of the plugboard 5-3 is spliced in the slot 2-1, so that the cover plate moving plate 5-1 is connected with the cover plate 2, preferably, the cover plate moving plate 5-1 is slidably mounted on the carrying platform 1 through the sliding rail 5-4, the sliding rail 5-4 is obliquely arranged, the plugboard 5-3 is inclined towards the moving direction of the cover plate 2, the plugboard 5-3 generates partial downward acting force (the acting force cannot tilt the cover plate 2) on the cover plate 2, and the cover plate moving plate 5-1 can be stably driven to move the cover plate 2.
The bubble removing mechanism 6 comprises a bubble removing driver 6-1 and a top plate 6-2, the top plate 6-2 is installed on the carrying platform 1 in a lifting mode through an upright post 6-4, the bubble removing driver 6-1 is connected with the top plate 6-2, the top plate 6-2 is driven to lift, a supporting plate 6-3 is bent upwards in the middle of the top plate 6-2, the supporting plate 6-3 is abutted to the lower end face of a connector 2-3 of the cover plate 2, the bubble removing driver 6-1 can adopt one of an oil cylinder, an air cylinder and an electric cylinder, the top plate 6-2 is driven to lift through the bubble removing driver 6-1, one side of the cover plate 2 is jacked up, reagents are gathered towards the overlapping direction of a glass slide and the cover plate 2, and when the cover plate 2 is covered again, the reagents are distributed in gaps between the glass slide and the cover plate 2 again, and the bubble removing effect is achieved.
The top plate 6-2 is arranged below the liquid draining mechanism 5, the jacking height of the top plate 6-2 is limited through the liquid draining mechanism 5, and when the cover plate 2 is jacked, the inclination angle between the cover plate 2 and the glass slide is ensured to be within 10 degrees, so that the bubble removing effect is improved.
The sample adding mechanism 8 comprises a sample adding driving piece 8-1 and a sample adding head 8-2, the sample adding head 8-2 is arranged on the sample adding driving piece 8-1, the sample adding head 8-2 is driven to lift by the sample adding driving piece 8-1, the sample adding driving piece 8-1 comprises a stepping motor, a screw rod is arranged on the stepping motor, a sliding block is arranged on the screw rod, the sample adding head 8-2 is connected with the sliding block through a connecting piece 8-3, the sample adding head 8-2 is driven to move up and down by the stepping motor, and when the sample adding head 8-2 is abutted to the joint of the cover plate 2 and the glass slide, reagents in the sample adding head 8-2 flow between the cover plate 2 and the glass slide through capillary siphoning.
The device comprises the following working conditions,
(1) The cover plate 2 is covered on the glass slide, and the cover plate mechanism 4 is driven to descend by the driving mechanism 3, so that the pressing frame 4-2 is pressed on the cover plate 2, and the cover plate 2 is abutted on the glass slide.
(2) After the previous test is completed, the reagent of the previous test is discharged from the glass slide; the cover plate moving plate 5-1 is driven to move by the driving mechanism 3, the cover plate 2 moves along with the cover plate moving plate 5-1, liquid is taken away from the glass slide when the cover plate moves outside the glass slide due to the action of reagent tension, and meanwhile, the air draft device works, so that the negative pressure cavity 1-1 is in a negative pressure state, and reagents are conveniently discharged from between the cover plate and the glass slide through negative pressure in the negative pressure cavity.
(3) Bubble removal operation; the top plate 6-2 is driven to move upwards by the bubble removal driver 6-1, one side of the cover plate 2 is jacked up by the top plate 6-2, so that reagents are gathered towards the overlapping direction of the glass slide and the cover plate 2, and when the cover plate 2 is covered again, the reagents are distributed in gaps between the glass slide and the cover plate 2 again, so that the bubble removal effect is realized.
(4) Adding a reagent; the sample adding head 8-2 is driven to move up and down by a stepping motor, and when the sample adding head 8-2 is abutted to the joint of the cover plate 2 and the glass slide, reagents in the sample adding head 8-2 flow into the space between the cover plate 2 and the glass slide by capillary siphoning.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention; thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Although the terms corresponding to the reference numerals in the drawings are used more herein, the possibility of using other terms is not excluded; these terms are used merely for convenience in describing and explaining the nature of the invention; they are to be interpreted as any additional limitation that is not inconsistent with the spirit of the present invention.
Claims (10)
1. An immunohistochemical analysis appearance slide glass loading device, characterized by including
The device comprises a carrying platform (1), wherein a cover plate (2) is assembled on the carrying platform, the cover plate (2) is matched with a glass slide, a sample is fixed on the glass slide, a negative pressure cavity (1-1) is arranged in the carrying platform (1), and a negative pressure port (1-2) is arranged on the negative pressure cavity (1-1);
a driving mechanism (3);
the cover plate mechanism (4) is connected with the driving mechanism (3), and the driving mechanism (3) drives the cover plate mechanism (4) to move relative to the carrying platform (1) so as to fix the cover plate (2) on the carrying platform (1);
the liquid discharging mechanism (5) is connected with the cover plate (2), the liquid discharging mechanism (5) is driven to move through the driving mechanism (3), the cover plate (2) is driven to move towards the negative pressure port (1-2) of the negative pressure cavity (1-1), so that liquid between the cover plate (2) and the glass slide is discharged through the negative pressure port (1-2), the negative pressure cavity (1-1) is connected with a plurality of negative pressure connectors (1-12), and the negative pressure cavity is pumped through the negative pressure connectors (1-12) to ensure that the negative pressure in the negative pressure cavity (1-1) is uniform;
the bubble removing mechanism (6) is matched with the cover plate (2), and one side of the cover plate (2) is jacked up through the bubble removing mechanism (6) so that the cover plate (2) is inclined relative to the glass slide to discharge bubbles;
and a sampling mechanism (8) for adding a reagent to the slide.
2. The slide loading device of the immunohistochemical analyzer according to claim 1, wherein the carrying platform (1) comprises a base (1-3) and a negative pressure box body (1-4) spliced on the base (1-3) for forming a negative pressure cavity (1-1), a gas-liquid separation plate (1-5) is arranged in the negative pressure box body (1-4), the gas-liquid separation plate is arranged below the negative pressure port (1-2), and a ventilation groove (1-6) is arranged on the gas-liquid separation plate (1-5).
3. The slide loading device of the immunohistochemical analyzer according to claim 2, wherein the gas-liquid separation plate (1-5) is obliquely arranged on the negative pressure box body (1-4), a first guide plate (1-7) and a second guide plate (1-8) are respectively connected to the high side and the low side of the gas-liquid separation plate (1-5), the height of the second guide plate (1-8) is higher than that of the first guide plate (1-7) and that of the gas-liquid separation plate (1-5), the vent groove (1-6) is arranged at the top of the second guide plate (1-8), and a liquid outlet (1-9) is formed in the gas-liquid separation plate (1-5). Baffle plates (1-10) are connected to the inclined two sides of the first guide plate (1-7) and the gas-liquid separation plate (1-5), the liquid discharge cavity is formed by encircling the first guide plate (1-7), the gas-liquid separation plate (1-5) and the baffle plates (1-10), and the liquid discharge port (1-9) is arranged in the liquid discharge cavity.
4. The slide loading device of the immunohistochemical analyzer according to claim 2, wherein a slide table (7) is arranged on the negative pressure box body (1-4), the slide table (7) comprises a mounting base (7-1) and a placing seat (7-2) arranged on the mounting base (7-1), a waste discharge port (7-3) is arranged on the placing seat (7-2), a waste discharge pipe (7-4) is arranged on the mounting base (7-1), the waste discharge port (7-3) is connected with the waste discharge pipe (7-4) to form the negative pressure port (1-2), and the tail end position of the waste discharge pipe (7-4) is lower than the ventilation groove (1-6).
5. The slide loading device of the immunohistochemical analyzer according to claim 1, wherein the liquid draining mechanism (5) comprises a cover plate moving plate (5-1), the cover plate moving plate (5-1) is inserted into the cover plate (2), the driving mechanism (3) is connected with the cover plate moving plate (5-1), and the driving mechanism (3) drives the cover plate moving plate (5-1) to translate relative to the slide so that liquid between the cover plate (2) and the slide moves to the position above the negative pressure port (1-2) along with the cover plate.
6. The slide loading device of the immunohistochemical analyzer according to claim 1, wherein the bubble removing mechanism (6) comprises a bubble removing driver (6-1) and a top plate (6-2), the top plate (6-2) is installed on the carrying platform (1) in a lifting mode, the bubble removing driver (6-1) is connected with the top plate (6-2) to drive the top plate (6-2) to lift, a supporting plate (6-3) is arranged on the top plate (6-2), and the supporting plate (6-3) is matched with the cover plate (2).
7. The slide loading device for the immunohistochemical analyzer according to claim 6, wherein the slot (2-1) is arranged on the cover plate (2), one side of the cover plate (2) is slidably connected with the liquid draining mechanism (5) through the slot (2-1), and the supporting plate (6-3) is abutted against one side of the slot (2-1) so as to jack up the cover plate (2) through the supporting plate (6-3).
8. The slide loading device for an immunohistochemical analyzer according to claim 7, wherein the top plate (6-2) is disposed below the liquid discharging mechanism (5), and the jacking height of the top plate (6-2) is limited by the liquid discharging mechanism (5).
9. The slide loading device of the immunohistochemical analyzer according to claim 1, wherein the cover plate mechanism (4) comprises a cover plate (4-1) and a pressing frame (4-2), the cover plate (4-1) is installed on the carrying platform (1) in a lifting manner through a guide assembly (9), the pressing frame (4-2) is arranged on the inner side end face of the cover plate (4-1), and the cover plate (2) is pressed through the pressing frame (4-2).
10. The slide loading device of the immunohistochemical analyzer according to claim 1, wherein the sample loading mechanism (8) comprises a sample loading driving piece (8-1) and a sample loading head (8-2), the sample loading head (8-2) is arranged on the sample loading driving piece (8-1), and the sample loading head (8-2) is driven to lift by the sample loading driving piece (8-1).
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| CN202311124396.6A CN117168940A (en) | 2023-09-01 | 2023-09-01 | Slide loading device of immunohistochemical analyzer |
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| CN202311124396.6A CN117168940A (en) | 2023-09-01 | 2023-09-01 | Slide loading device of immunohistochemical analyzer |
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| CN116046504A (en) * | 2022-05-07 | 2023-05-02 | 武汉纺织大学 | Full-automatic immunohistochemical dyeing apparatus |
| CN116481879A (en) * | 2023-04-06 | 2023-07-25 | 浙江莱阅病理诊断科技有限公司 | Immunohistochemical staining method |
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| JPH03111035A (en) * | 1989-09-25 | 1991-05-10 | Chiyoda Seisakusho:Kk | Dyeing device and dyeing tray |
| CN111562164A (en) * | 2020-05-15 | 2020-08-21 | 河南赛诺特生物技术有限公司 | Liquid cover film |
| CN116046504A (en) * | 2022-05-07 | 2023-05-02 | 武汉纺织大学 | Full-automatic immunohistochemical dyeing apparatus |
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