CN115184122A - Automatic staining instrument slide carrying platform device and using method thereof - Google Patents
Automatic staining instrument slide carrying platform device and using method thereof Download PDFInfo
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- CN115184122A CN115184122A CN202210852791.5A CN202210852791A CN115184122A CN 115184122 A CN115184122 A CN 115184122A CN 202210852791 A CN202210852791 A CN 202210852791A CN 115184122 A CN115184122 A CN 115184122A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
Abstract
The invention discloses a slide carrying platform device of an automatic staining instrument and a using method thereof, and the slide carrying platform device comprises a rack, a heating platform, a heating sheet and a reaction cover plate, wherein the reaction cover plate comprises a liquid adding area, a reaction area, a drainage area and a liquid gathering area, the liquid adding area, the reaction area and the drainage area are sequentially arranged on the reaction cover plate in a stepped manner, the liquid gathering area is connected with the drainage area, and the reaction area of the reaction cover plate and the reaction area of the reaction slide are matched to form an anti-dry slide reaction chamber for absorbing liquid gathering by applying a siphon principle.
Description
Technical Field
The invention relates to the field of cell slide dyeing equipment, in particular to a slide carrying table device of an automatic dyeing instrument and a using method thereof.
Background
At present, in the in-process of medical field cell dyeing, all can have the washing, the liquid feeding is hatched, the heating repair flow, under the ordinary condition, the slide that scribbles the cell can artifical reaction liquid such as dropwise add antibody, after hatching at wet box normal atmospheric temperature, put the washing jar again and soak and wash, because reaction liquid such as dropwise add antibody generally is multiple, whole process operation step is many and repeated, reagent is extravagant big, the step standard is inconsistent, easy error, it is inevitable trend to use full-automatic dyeing machine to replace the manual work, the core solution key element of dyeing machine is the slide microscope carrier that is applicable to the cell dyeing, but the dyeing machine can not be complete the operation of imitating people the same, in practice need solve the cooling after the heating repair, the waste liquid is collected, accomplish both and compromise, and the structure is uncomplicated.
In summary, there is a need for an automatic staining apparatus slide stage apparatus and a method of using the same to address the deficiencies of the prior art.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a slide carrying table device of an automatic staining instrument and a using method thereof, aiming at solving the problems.
In order to achieve the purpose, the invention provides the following technical scheme: an automatic dyeing instrument slide carrier device is matched with a slide pulling mechanism to act and comprises a workbench, a rack, heating tables and a waste liquid extracting device, wherein the rack is averagely provided with a plurality of heating tables, one end of each heating table is correspondingly provided with a liquid discharging block, the liquid discharging block is provided with a liquid discharging cavity, the lower end of the liquid discharging cavity is connected with the waste liquid extracting device, the liquid discharging cavity extracts waste liquid on a reaction slide through a reaction cover plate by utilizing liquid viscosity, a corresponding reaction cover plate is arranged above each heating table, one end of the reaction cover plate is detachably connected with the slide pulling mechanism, the reaction slide for detection is placed on the heating tables, when the automatic dyeing instrument slide carrier device is used, the reaction cover plate is covered on the reaction slide, the reaction cover plate is corresponding to different working stages by adjusting the position of the reaction cover plate relative to the reaction slide for detection, the reaction cover plate comprises a liquid adding area, a reaction area, a flow guiding area and a liquid collecting area, the liquid adding area, the reaction area, the flow guiding area and the liquid collecting area are sequentially arranged, the liquid collecting area on the reaction cover plate is a concave area with the reaction area with the depth smaller than 1mm, the surface of the reaction slide, the reaction area is horizontally arranged, the surface of the slide, the liquid collecting area is matched with a liquid collecting area, and the reaction slide, and the reaction area is matched with a liquid collecting area, and a liquid collecting area matched with a liquid collecting area.
Optionally, the heating table is rectangular in whole, a plurality of parallel heating grooves are formed in the middle section of the upper surface of the heating table, and the upper surfaces of all the heating grooves are attached to the lower surface of the reaction slide when in use.
Optionally, the flow guiding region of the reaction cover slip or/and the reaction slide is a hydrophilic material region, and when the waste liquid is extracted, after the cover slip is pulled, the slide reaction region of the reaction slide, the reaction cover slip and the flow guiding region of the reaction slide form a waste liquid extraction channel under negative pressure.
Optionally, still be provided with the buffer on the reaction cover plate, the liquid gathers the district and is connected with the drainage district, the buffer sets up between liquid feeding district and reaction zone and drainage district, the liquid feeding district includes liquid filling opening and liquid inlet district, the liquid filling opening sets up liquid feeding district edge.
Optionally, the pull-tab mechanism includes a pull-tab base plate, a pull plate is disposed on one side of the pull-tab base plate, a fixing plate is disposed on the other side of the pull-tab base plate, the pull plate and the fixing plate are fixedly connected to the pull-tab base plate, a fastening bump is disposed on the fixing plate, a fastening portion is further disposed on the reaction cover plate, the fastening portion is connected to the liquid collection area, and the fastening portion corresponds to the fastening bump.
Optionally, the waste liquid extraction device includes that a ventilation flowing back chamber is arranged in the rack, the ventilation flowing back chamber is a closed space formed by the workbench and the rack together, a liquid discharge groove is arranged in the rack, the liquid discharge groove is communicated with the ventilation flowing back chamber, and the liquid discharge groove corresponds to the liquid discharge block.
Optionally, the workstation below sets up the flowing back funnel, flowing back funnel and workstation fixed connection, the flowing back funnel is linked together with the flowing back groove, flowing back funnel one side sets up the exhaust column, the exhaust column is linked together with the ventilation flowing back chamber, exhaust column and workstation fixed connection, connect the diaphragm pump on the flowing back funnel, the water inlet and the flowing back funnel of diaphragm pump can be dismantled and be connected, the delivery port and the waste liquid bucket of diaphragm pump are connected.
Optionally, set up the flowing back fan on the exhaust column, the flowing back fan can be dismantled with the exhaust column and be connected, the flowing back fan carries out the low-speed and airs exhaust for reaction slide 100 restoration backs, reaches the flowing back fan of the supplementary cooling effect of hot plate.
Optionally, the rack includes an upper drainage rack and a lower drainage ring, the upper drainage rack is detachably connected with the lower drainage ring, the drainage groove is formed in the inner side wall of the lower sealing ring, the liquid collection area is provided with a drainage guide block, the drainage guide block is fixedly connected with the reaction cover plate, the drainage block is provided with a limiting step, and the limiting step is arranged on one side where the drainage block is connected with the heating table.
A use method of a slide carrier device of an automatic staining instrument comprises the following steps:
s1, a step: checking a slide carrying table device of the automatic dyeing instrument, installing a reaction slide on a heating table after the error is confirmed, and fixing a reaction cover plate on a sheet pulling mechanism;
and S2, a step: repairing a reaction slide, adjusting the angle of a carrier, keeping the inclination of about 1 degree, forming a reaction area of a reaction cover plate and a slide reaction area of the reaction slide to be matched to form an anti-dry slide reaction chamber for sucking liquid to gather by applying a siphon principle, sucking cell repairing liquid to be dripped into a liquid adding area at the front part of the reaction cover plate, introducing the repairing liquid into the reaction chamber under the siphon action, starting a heating table, heating the repairing liquid by 90-100 degrees, keeping for 30 minutes, and completing the repairing of the cell slide;
and S3, a step: cleaning a reaction slide, adjusting the angle of a carrier, keeping the inclination of 1-10 degrees, pushing a sheet pulling mechanism to enable a buffer zone and a drainage zone between a reaction zone and the drainage zone on the reaction cover plate to contact a slide reaction zone defined by a hydrophobic coating, sucking cleaning liquid and adding the cleaning liquid to a liquid adding zone of the reaction cover plate, enabling the cleaning agent to flow through a slide reaction zone defined by the hydrophobic coating on the reaction slide under the siphon principle action of the drainage zone and the buffer zone, and finally gathering the cleaning agent into liquid drops in a liquid gathering zone;
and S4, a step: starting a waste liquid extraction device, exhausting air by an exhaust fan through an exhaust pipe to form negative pressure in a ventilation liquid discharge cavity, enabling liquid on a reaction cover plate to enter a drainage groove in a rack through a liquid discharge cavity on a liquid discharge block, enabling the liquid to enter a liquid discharge funnel through the drainage groove, finally pumping the liquid into a waste liquid barrel through a diaphragm pump to complete the cleaning of a reaction slide for one time, and repeating the steps for 2-4 times to complete the whole cleaning process of the reaction slide;
and S4, a step: moving the sheet pulling mechanism, driving the reaction cover plate and the reaction glass slide to move relatively by the sheet pulling mechanism, separating a liquid adding area of the reaction cover plate from a glass slide reaction area defined by a hydrophobic coating of the reaction glass slide, and adding an antibody reagent into the glass slide reaction area defined by the hydrophobic coating;
and S5, a step: and pushing the sheet pulling mechanism, driving the reaction cover plate and the reaction slide to move relatively by the sheet pulling mechanism, enabling the reaction area of the reaction cover plate to correspond to the slide reaction area defined by the hydrophobic coating of the reaction slide, and then carrying out heating incubation until the heating incubation is finished.
The invention has the beneficial effects that:
1. in the invention, the balance of liquid in the reaction zone is realized, the liquid is not greatly reduced due to evaporation, and dry sheets are not caused;
2. according to the invention, the automation of cell liquid adding repair is realized, compared with the manual liquid adding repair, the used reagents are fewer, the liquid adding repair is more stable, the reagents in the heating repair process are greatly saved, and the reagents for cleaning and heating incubation can also be saved in the same way;
3. according to the invention, the liquid accumulation area is designed on the reaction cover plate, so that redundant reagents or cleaning agents can be effectively accumulated, and then the reagents or cleaning agents are sucked through the low air pressure of the ventilation liquid discharge cavity, so that the reagents or cleaning agents fall into the liquid discharge groove, the redundant reagents or cleaning agents are convenient to collect, secondary pollution caused by the reagents and cleaning agents is prevented, and the problem that the reagents and cleaning agents are inconvenient to collect is effectively solved;
4. in the invention, the liquid drainage guide block on the liquid gathering area of the reaction cover plate can change the surface tension of liquid, so that the liquid can be drained from the reaction cover plate more easily, thereby ensuring the smoothness of liquid drainage and having certain use value and popularization value.
Drawings
Fig. 1 is a schematic perspective view of a slide stage apparatus according to the present invention.
FIG. 2 is a schematic plan view of the slide stage apparatus of the present invention.
Fig. 3 is a partial perspective view of the slide stage device of the present invention.
Fig. 4 is a schematic view of a partial plan view of the slide stage apparatus of the present invention.
FIG. 5 is a schematic view of a reaction patch of the present invention.
Fig. 6 is a schematic plan view of another configuration of the slide stage device of the present invention.
FIG. 7 is a schematic view of a step-cross section of the slide stage apparatus of the present invention.
FIG. 8 is a schematic diagram showing a reaction cover plate and a reaction slide in a liquid-applied state according to the present invention.
FIG. 9 is a view showing a structure of a reaction slide and a reaction cover plate according to the present invention in a reaction state.
FIG. 10 is a view showing a configuration of the reaction cover plate and the reaction slide in a liquid-discharging state according to the present invention.
In the figure: 1. a work table; 2. a rack; 3. a heating stage; 31. heating the groove; 4. draining the liquid block; 41. a limiting step; 5. a liquid discharge cavity; 6. a reaction cover plate; 7. a liquid discharge funnel; 8. an exhaust pipe; 9. a diaphragm pump; 10. a liquid discharge fan; 11. a waste liquid barrel; 12. reaction glass slide; 121. a slide reaction zone; 122. a hydrophobic coating ring; 13. a pull tab mechanism; 131. a pull tab chassis; 132. pulling the movable plate; 133. a fixing plate; 134. connecting the bumps; 21. a ventilation liquid discharge cavity; 22. a liquid discharge tank; 23. liquid blocking ribs; 24. an upper drainage rack; 25. a lower drainage ring; 61. a liquid feeding area; 611. a liquid filling port; 612. a liquid inlet zone; 62. a reaction zone; 63. a diversion area; 64. a liquid accumulation zone; 65. a buckling part; 66. a buffer area; 67. and a liquid drainage guide block.
Detailed Description
As shown in fig. 1-7, an automatic dyeing appearance slide microscope carrier device, with the cooperation action of pulling-on piece mechanism 13, comprises a workbench 1, rack 2, warm table 3 and waste liquid draw-out device, on average dispose a plurality of warm table 3 on the rack 2, warm table 3 wholly is the rectangle, a plurality of heating recess 31 that are parallel to each other are seted up to the middle section of warm table 3 upper surface, all laminate with the lower surface of reaction slide when the upper surface of all heating recess 31 uses, the one end correspondence of warm table 3 is provided with drainage piece 4, drainage chamber 5 has been seted up on drainage piece 4, the lower extreme and the waste liquid draw-out device of drainage chamber 5 are connected, drainage chamber 5 utilizes the liquid stickness to extract through reaction cover plate 6 the waste liquid on reaction slide 12, all be provided with corresponding reaction cover plate 6 above every warm table 3, 6 one end of reaction cover plate can be dismantled with pulling-on piece mechanism 13 and be connected.
The reaction slide 12 for detection is placed on the heating platform 3, when in use, the reaction cover plate 6 is attached and covered on the reaction slide 12, different working stages are corresponded by adjusting the position of the reaction cover plate 6 relative to the reaction slide 12 for detection, the reaction cover plate 6 comprises a liquid adding area 61, a reaction area 62, a drainage area 63 and a liquid gathering area 64, the liquid adding area 61, the reaction area 62, the drainage area 63 and the liquid gathering area 64 are sequentially arranged in a step shape, the reaction area 62 on the reaction cover plate 6 is an upper concave area with the depth less than 1mm, the surface of the reaction slide 12 is horizontally configured, the reaction area 62 on the reaction slide 12 corresponding to the reaction cover plate 6 is provided with a slide reaction area 121 defined by a hydrophobic coating, the drainage area of the reaction cover plate 6 or/and the reaction slide 12 is a hydrophilic material area, when waste liquid is extracted, after the reaction cover plate 6 is pulled, the slide reaction zone 121 of the reaction slide 12, the reaction cover plate 6 and the drainage zone 63 of the reaction slide 6 form a waste liquid extraction channel under negative pressure, the liquid collection zone 64 is connected with the drainage zone 63, the reaction zone 62 of the reaction cover plate 6 and the slide reaction zone 121 of the reaction slide 12 are matched to form an anti-drying reaction chamber for sucking liquid collection by applying a siphon principle, the reaction cover plate 6 is further provided with a buffer zone 66, the liquid collection zone 64 is connected with the drainage zone 63, the buffer zone 66 is arranged between the liquid feeding zone 61 and the reaction zone 62 and between the reaction zone 62 and the drainage zone 63, the liquid feeding zone 61 comprises a liquid feeding port 611 and a liquid feeding zone 612, and the liquid feeding port 611 is arranged at the edge of the liquid feeding zone 612.
The pull-tab mechanism 13 comprises a pull-tab bottom plate 131, a pull plate 132 is arranged on one side of the pull-tab bottom plate 131, a fixing plate 133 is arranged on the other side of the pull-tab bottom plate 131, the pull plate 132 and the fixing plate 133 are fixedly connected with the pull-tab bottom plate 131, a buckling bump 134 is arranged on the fixing plate 133, a buckling part 65 is further arranged on the reaction cover plate 6, the buckling part 65 is connected with the liquid gathering area 64, and the buckling part 65 corresponds to the buckling bump 134.
Waste liquid draw-off mechanism includes that 2 interior settings of platform ventilate flowing back chamber 21, ventilate flowing back chamber 21 is the common enclosure space that forms of workstation and rack, set up flowing back groove 22 in the rack 2, flowing back groove 22 is linked together with ventilation flowing back chamber 21, flowing back groove 22 is corresponding with flowing back piece 4, platform 2 includes last flowing back frame 24 and lower drainage ring 25, go up flowing back frame 24 and can dismantle with lower drainage ring 25 and be connected, flowing back groove 22 sets up on lower sealing ring 25 inside wall, set up flowing back guide block 67 on the liquid gathering area 64, flowing back guide block 67 and 6 fixed connection of reaction cover plate, set up spacing halfpace 41 on flowing back piece 4, spacing halfpace 41 sets up the one side that is connected at flowing back piece 4 and warm table 3, workstation 1 below sets up flowing back funnel 7, flowing back funnel 7 and workstation 1 fixed connection, flowing back funnel 7 and flowing back funnel 7 are linked together, flowing back 7 are linked together, 7 one side sets up exhaust pipe 8, exhaust pipe 8 and ventilation flowing back runback draft pipe 21 are linked together, exhaust pipe 8 and the auxiliary cooling fan is carried out 10 to the auxiliary cooling fan on the flowing back.
A method for using a slide carrier device of an automatic staining instrument comprises the following steps:
s1, a step: checking the slide carrying platform device of the automatic dyeing instrument, installing the reaction slide on a heating platform after the error is confirmed, and fixing the reaction cover plate on a slide pulling mechanism;
and S2, a step: repairing a reaction slide, adjusting the angle of a carrier, keeping the inclination of about 1 degree, forming a reaction area of a reaction cover plate and a slide reaction area of the reaction slide to be matched to form a dry-proof reaction chamber for attracting liquid to gather by applying a siphon principle, sucking cell repairing liquid to be dripped into a liquid adding area at the front part of the reaction cover plate, introducing the repairing liquid into the reaction chamber under the siphon action, starting a heating table, heating the repairing liquid by 90-100 degrees, keeping the temperature for 30 minutes, completing the repair of the cell slide, starting a liquid discharge fan of a waste liquid extraction device, and exhausting air at low speed by the liquid discharge fan to achieve the effect of auxiliary cooling of the heating table;
and S3, a step: cleaning a reaction slide, adjusting the angle of a carrier, keeping the inclination of 1-10 degrees, pushing a sheet pulling mechanism to enable a buffer area and a drainage area between a reaction area and a drainage area on the reaction cover plate to be in contact with a slide reaction area defined by a hydrophobic coating, drawing 10, sucking cleaning liquid and adding the cleaning liquid into a liquid adding area of the reaction cover plate, enabling the cleaning liquid to flow through the slide reaction area defined by the hydrophobic coating on the reaction slide under the action of the siphon principle of the drainage area and the buffer area, and finally gathering the cleaning liquid into liquid drops in a liquid gathering area;
and S4, a step: starting a waste liquid extraction device, exhausting air by a liquid exhaust fan through an exhaust pipe to form negative pressure in a ventilation liquid exhaust cavity, enabling liquid on a reaction cover plate to enter a drainage groove in a rack through a liquid exhaust cavity on a liquid exhaust block, enabling the liquid to enter a liquid exhaust funnel through the drainage groove, finally pumping the liquid into a waste liquid barrel through a diaphragm pump to complete the cleaning of a reaction slide once, and repeating for 2-4 times to complete the whole cleaning process of the reaction slide;
and S4, a step: moving the sheet pulling mechanism, driving the reaction cover plate and the reaction glass slide to move relatively by the sheet pulling mechanism, separating a liquid adding area of the reaction cover plate from a glass slide reaction area defined by the hydrophobic coating of the reaction glass slide, and adding an antibody reagent into the glass slide reaction area defined by the hydrophobic coating, as shown in figure 8;
and S5, a step: and pushing the sheet pulling mechanism, driving the reaction cover plate and the reaction slide to move relatively by the sheet pulling mechanism, enabling the reaction area of the reaction cover plate to correspond to the slide reaction area defined by the hydrophobic coating of the reaction slide, and then carrying out heating incubation until the heating incubation is finished, as shown in the attached figure 9.
The working principle of the invention is as follows: when the cell staining in-process slide liquid feeding is restoreed, the prosthetic reaction slide of liquid feeding will be required at present and placed on the warm table, the reaction slide is the horizontal configuration, there is hydrophobic coating circle on the reaction slide, make reagent reachd the reaction zone more easily, position fixing through spacing halfpace on the flowing back piece with the reaction slide, prevent that the slide loading platform is when the slope, the removal of reaction slide, stability when effectual assurance liquid feeding is restoreed and is washd, place the reaction cover plate in the reaction slide top again, it is fixed with the buckling part of reaction cover plate to construct the connection lug of upper fixed plate through the pulling-on piece, make things convenient for the installation and the dismantlement of reaction cover plate and fixed plate.
After the cell slide repairing device is installed, a mechanical arm sucks cell repairing liquid to be added to a liquid adding port of a liquid adding area in front of a cover plate, liquid enters the liquid inlet area through the liquid adding port, the sheet pulling mechanism is pushed to drive a reaction cover plate, when a reaction area of the reaction cover plate corresponds to the reaction area of the reaction slide, liquid flows to the reaction area through a buffer area, a reaction cavity formed by the reaction slide and the reaction cover plate sucks liquid to gather in the middle of a reaction cavity and keeps 1-degree horizontal inclination, a liquid drainage gathering area and a drainage area at the lower part of the reaction cover plate form a liquid siphon effect, when the cell slide is repaired, the liquid in the reaction area is heated to 90-100 degrees and kept for 30 minutes, and due to the fact that a heating groove is arranged on the heating plate, adsorption cannot be caused by the fact that a heating surface is a plane, the reaction slide is convenient to take down from the heating plate, the liquid can be partially evaporated, the liquid flowing to the lower part of the cover plate and the drainage area can supplement the liquid to the reaction area, balance of the liquid in the reaction area is achieved, the liquid can be reduced greatly due to evaporation, dry plates, a cell slide repairing process is achieved, and a cell slide repairing reagent used more stably added than a liquid adding process is saved.
The buffer zone sets up between liquid feeding district and reaction zone and drainage district, when liquid flows into the reaction zone from the liquid feeding district, play the cushioning effect, prevent the liquid flow rate at the excessive speed, on the same hand, when liquid is from the reaction zone to the drainage district, also play the cushioning effect, when needing the pulling cover plate, drive the pulling-on piece bottom plate through the pulling plate, the pulling-on piece bottom plate drives the fixed plate and removes, realize the pulling of reaction cover plate, the flowing back piece is used for discharging unnecessary reagent or washing reagent, make things convenient for cell liquid feeding to restore and wash among the cell dyeing process.
When the cleaning machine is used for cleaning, the workbench inclines by 1-10 degrees, the mechanical arm is dropwise added with cleaning liquid at a liquid adding port in the front of the reaction cover plate, the liquid is discharged after passing through the reaction chamber, the lower part of a liquid discharging port of the workbench has an air draft function, the air draft function is realized through an air draft pipe and a liquid discharging fan, the fan is started during liquid discharging, a liquid discharging diversion area of the cover plate is contacted with the liquid in a middle circle of the slide, the liquid is sucked to a liquid discharging accumulation area by a chamber of the liquid guiding area and forms water drops in the accumulation area at the lower part of the cover plate, the formed water drops are sucked by air draft of a liquid discharging cavity of a liquid discharging block after the fan is started, a liquid discharging guide block is further arranged on the liquid collecting area, the liquid drops are sucked more easily, the cleaning liquid and the reaction waste liquid are discharged rapidly and enter a liquid discharging groove in the rack, the cleaning machine and the reaction waste liquid are discharged into a waste liquid barrel under the action of a liquid discharging funnel and a diaphragm pump below the workbench, and the cleaning machine is repeated for 2-4 times to complete the cleaning process.
The liquid discharge funnel collects liquid such as reagent or cleaning liquid in the liquid discharge groove, and liquid in the liquid discharge groove can flow out conveniently. The exhaust column is used for convulsions, make the interior low atmospheric pressure that forms of ventilation flowing back intracavity, thereby make things convenient for liquid to flow into the flowing back groove, the diaphragm pump does not have the bearing seal, no leakage, no rotating member, the trafficability characteristic is good, to volatilizing or corrosive reagent, the transport of diaphragm pump can not cause environmental pollution and harm personal safety, the exhaust column is connected to the flowing back air exhauster, the flowing back air exhauster during operation, through the gas of exhaust column suction ventilation flowing back intracavity, form the low atmospheric pressure in the flowing back wall that makes the ventilation, thereby make the liquid of the liquid gathering area gathering on the reaction cover plate fall into the flowing back groove, make things convenient for the discharge of reagent or cleaner.
The flow guiding area of the reaction cover plate or/and the reaction slide is a hydrophilic material area, namely the first flow guiding area of the reaction cover plate is a hydrophilic material area, and the flow guiding area of the reaction slide is a Teflon hydrophobic material area; the second kind is hydrophilic material district for the drainage zone of reaction cover plate and reaction slide, it is better to drain fluid effect like this, the slide reaction zone that hydrophobic coating on the reaction slide of above two kinds of condition was circled is hydrophilic material district, because the design of flowing back tank, the integrative inconvenient processing of rack, therefore, divide into drain rack and lower drainage ring on with the rack, so both make things convenient for rack generation processing, rack installation and dismantlement have been made things convenient for again, it can effectually place reagent or cleaner and get into the exhaust column to hinder the liquid muscle, get into the air exhauster even, can the effectual life who guarantees the air exhauster.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents or improvements made within the spirit and principle of the present invention should be included in the scope of the present invention.
Claims (10)
1. An automatic dyeing instrument slide carrier device, which is matched with a slide pulling mechanism to act, comprises a workbench, a rack, heating tables and a waste liquid extracting device, wherein the rack is averagely provided with a plurality of heating tables, and is characterized in that one end of each heating table is correspondingly provided with a liquid discharging block, the liquid discharging block is provided with a liquid discharging cavity, the lower end of the liquid discharging cavity is connected with the waste liquid extracting device, the liquid discharging cavity extracts waste liquid on a reaction slide through a reaction cover plate by utilizing liquid viscosity, a corresponding reaction cover plate is arranged above each heating table, one end of the reaction cover plate is detachably connected with the slide pulling mechanism, the reaction slide for detection is placed on the heating tables, and the reaction cover plate is covered on the reaction slide when in use, the position of a reaction cover plate relative to a reaction slide for detection is adjusted to correspond to different working stages, the reaction cover plate comprises a liquid adding area, a reaction area, a flow guiding area and a liquid gathering area, the liquid adding area, the reaction area, the flow guiding area and the liquid gathering area are sequentially arranged in a stepped mode, the liquid gathering area is connected with the flow guiding area, the reaction area on the reaction cover plate is an upper concave area with the depth smaller than 1mm, the surface of the reaction slide is horizontally arranged, the position, corresponding to the reaction area on the reaction cover plate, of the reaction slide is provided with a slide reaction area defined by a hydrophobic coating, and the reaction area of the reaction cover plate and the slide reaction area of the reaction slide are matched to form an anti-dry slide reaction chamber for attracting liquid gathering by applying a siphon principle.
2. The automatic staining instrument slide carrier device according to claim 1, wherein the heating table is rectangular in shape, a plurality of parallel heating grooves are formed in the middle section of the upper surface of the heating table, and the upper surfaces of all the heating grooves are attached to the lower surface of the reaction slide when in use.
3. The slide carrier apparatus of an automatic stainer of claim 1, wherein the reaction cover plate or/and the drainage area of the reaction slide are hydrophilic material areas, and the slide reaction area of the reaction slide, the reaction cover plate and the drainage area of the reaction slide form a waste liquid extraction channel under negative pressure after the reaction cover plate is pulled during waste liquid extraction.
4. The automatic staining instrument slide carrier device according to claim 1, wherein the reaction cover plate further comprises a buffer area, the liquid collection area is connected with the drainage area, the buffer area is arranged between the liquid feeding area and the reaction area, and between the reaction area and the drainage area, the liquid feeding area comprises a liquid feeding port and a liquid feeding area, and the liquid feeding port is arranged at the edge of the liquid feeding area.
5. The automatic stainer slide carrying platform device as claimed in claim 1, wherein the pull-on mechanism comprises a pull-on bottom plate, a pull plate is disposed on one side of the pull-on bottom plate, a fixing plate is disposed on the other side of the pull-on bottom plate, the pull plate and the fixing plate are fixedly connected to the pull-on bottom plate, a fastening protrusion is disposed on the fixing plate, a fastening portion is further disposed on the reaction cover plate, the fastening portion is connected to the liquid collection area, and the fastening portion corresponds to the fastening protrusion.
6. The automatic staining instrument slide carrier device as claimed in claim 1, wherein the waste liquid extraction device comprises a ventilation and liquid drainage cavity arranged in the rack, the ventilation and liquid drainage cavity is a closed space formed by the workbench and the rack, a liquid drainage groove is arranged in the rack, the liquid drainage groove is communicated with the ventilation and liquid drainage cavity, and the liquid drainage groove corresponds to the liquid drainage block.
7. The automatic staining instrument slide carrier device as claimed in claim 1, wherein a liquid discharge funnel is arranged below the workbench, the liquid discharge funnel is fixedly connected with the workbench, the liquid discharge funnel is communicated with the liquid discharge groove, an exhaust pipe is arranged on one side of the liquid discharge funnel, the exhaust pipe is communicated with the ventilation liquid discharge cavity, the exhaust pipe is fixedly connected with the workbench, a diaphragm pump is connected to the liquid discharge funnel, a water inlet of the diaphragm pump is detachably connected with the liquid discharge funnel, and a water outlet of the diaphragm pump is connected with the waste liquid barrel.
8. The slide carrier device of the automatic stainer as claimed in claim 7, wherein the exhaust tube is provided with a drain fan, the drain fan is detachably connected to the exhaust tube, and the drain fan performs low-speed air exhaust after 100 ° repair of the reaction slide, so as to achieve the effect of auxiliary cooling of the heating plate.
9. The automatic stainer slide carrier device as claimed in any one of claims 1-8, wherein the rack comprises an upper drainage rack and a lower drainage ring, the upper drainage rack is detachably connected with the lower drainage ring, the drainage groove is arranged on the inner side wall of the lower sealing ring, a drainage guide block is arranged on the liquid gathering area, the drainage guide block is fixedly connected with the reaction cover plate, a limiting step is arranged on the drainage block, and the limiting step is arranged on the side where the drainage block is connected with the heating table.
10. The use method of the slide carrier device of the automatic staining instrument is characterized by comprising the following steps:
s1, a step: checking the slide carrying platform device of the automatic dyeing instrument, installing the reaction slide on a heating platform after the error is confirmed, and fixing the reaction cover plate on a slide pulling mechanism;
and S2, a step: repairing a reaction slide, adjusting the angle of a carrier, keeping the carrier inclined at about 1 degree, forming a reaction area of a reaction cover plate and a slide reaction area of the reaction slide to be matched to form an anti-dry slide reaction chamber for sucking liquid to gather by applying a siphon principle, sucking cell repairing liquid to be dripped into a liquid adding area at the front part of the reaction cover plate, introducing the repairing liquid into the reaction chamber under the siphon action, starting a heating table, heating the repairing liquid by 90-100 degrees, keeping for 30 minutes, and completing the repairing of the cell slide;
and S3, a step: cleaning a reaction slide, adjusting the angle of a carrier, keeping the inclination of 1-10 degrees, pushing a sheet pulling mechanism to enable a buffer area and a drainage area between a reaction area and the drainage area on the reaction cover plate to be in contact with a slide reaction area defined by a hydrophobic coating, sucking cleaning liquid and adding the cleaning liquid into a liquid adding area of the reaction cover plate, enabling the cleaning liquid to flow through the slide reaction area defined by the hydrophobic coating on the reaction slide under the siphon principle action of the drainage area and the buffer area, and finally gathering the cleaning liquid into liquid drops in a liquid gathering area;
and S4, a step: starting a waste liquid extraction device, exhausting air by an exhaust fan through an exhaust pipe to form negative pressure in a ventilation liquid discharge cavity, enabling liquid on a reaction cover plate to enter a drainage groove in a rack through a liquid discharge cavity on a liquid discharge block, enabling the liquid to enter a liquid discharge funnel through the drainage groove, finally pumping the liquid into a waste liquid barrel through a diaphragm pump to complete the cleaning of a reaction slide for one time, and repeating the steps for 2-4 times to complete the whole cleaning process of the reaction slide;
and S4, a step: moving the sheet pulling mechanism, driving the reaction cover plate and the reaction slide to move relatively by the sheet pulling mechanism, separating a liquid adding area of the reaction cover plate from a slide reaction area defined by a hydrophobic coating of the reaction slide, and adding an antibody reagent into the slide reaction area defined by the hydrophobic coating;
and S5, a step: and pushing the sheet pulling mechanism, driving the reaction cover plate and the reaction slide to move relatively by the sheet pulling mechanism, enabling the reaction area of the reaction cover plate to correspond to the slide reaction area defined by the hydrophobic coating of the reaction slide, and then carrying out heating incubation until the heating incubation is finished.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210852791.5A CN115184122A (en) | 2022-07-19 | 2022-07-19 | Automatic staining instrument slide carrying platform device and using method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210852791.5A CN115184122A (en) | 2022-07-19 | 2022-07-19 | Automatic staining instrument slide carrying platform device and using method thereof |
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| CN115184122A true CN115184122A (en) | 2022-10-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202210852791.5A Pending CN115184122A (en) | 2022-07-19 | 2022-07-19 | Automatic staining instrument slide carrying platform device and using method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116008574A (en) * | 2022-12-14 | 2023-04-25 | 黑龙江八一农垦大学 | Immune group ization all-in-one |
| CN117168940A (en) * | 2023-09-01 | 2023-12-05 | 浙江莱阅病理诊断科技有限公司 | Slide loading device of immunohistochemical analyzer |
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2022
- 2022-07-19 CN CN202210852791.5A patent/CN115184122A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116008574A (en) * | 2022-12-14 | 2023-04-25 | 黑龙江八一农垦大学 | Immune group ization all-in-one |
| CN116008574B (en) * | 2022-12-14 | 2023-08-04 | 黑龙江八一农垦大学 | Immune group ization all-in-one |
| CN117168940A (en) * | 2023-09-01 | 2023-12-05 | 浙江莱阅病理诊断科技有限公司 | Slide loading device of immunohistochemical analyzer |
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