CN117101183A - Double-mechanical-arm type automatic solid-phase extraction system and application method thereof - Google Patents

Abstract

The invention discloses a double-mechanical arm type automatic solid phase extraction system and a use method thereof, wherein the double-mechanical arm type automatic solid phase extraction system comprises two groups of injection pump assemblies, two groups of solution buffer tube assemblies, two groups of ten-way valve assemblies, two groups of extraction column assemblies, two groups of mechanical arms, a transmission belt, two groups of sample injection mechanical arm assemblies, two groups of sample injection needles, two groups of solution collection mechanical arms, two groups of solution collection needles, two groups of leaching reagent groups, two groups of waste liquid collection bottles, two groups of sample pipe frames, solution collection pipe frames, two groups of waste liquid tanks and a control system; the invention can realize the application of separating and enriching chemical elements under multiple scenes, replaces manual liquid adding operation, realizes the coordination of a closed extraction column and a syringe pump, realizes adjustable extraction flow rate and improves experimental efficiency. The control system independently controls each component, and can simultaneously complete the operation of two different sample pretreatment processes. The serial columns can be completed by controlling one group of sample feeding mechanical arms to sample the collected solution in the solution collecting tube assembly.

Description

Double-mechanical-arm type automatic solid-phase extraction system and application method thereof

Technical Field

The invention relates to the technical field of solid-phase extraction experimental devices, in particular to a double-mechanical-arm type automatic solid-phase extraction system and a use method thereof.

Background

The multi-receiving inductively coupled plasma mass spectrometer (MC-ICP-MS) has the advantages of high analysis precision, small sample amount, high test speed and the like in the aspects of radioactive cause isotope and metal stable isotope analysis, samples in the fields of environment, geology, biology and the like have complex matrixes, and when digestion solutions of various samples directly test isotopes by using MC-ICP-MS, interference of matrix elements exists to influence accurate measurement of isotope composition. Therefore, obtaining a pure elemental composition to be measured by a chemical pretreatment method before on-machine testing of a sample solution is an important step. At present, solid phase extraction technology is often utilized to separate and purify elements to be detected and interfering elements in a sample, interference in the MC-ICP-MS test process is reduced, and high-precision isotope data is obtained.

The traditional manual solid phase extraction method needs to manually add sample solution and leaching reagent into the solid phase extraction column one by one, and the experimental process is tedious, time-consuming, long and the extraction time is uncontrollable. The automatic solid phase extraction technology for sample is to replace manual operation with mechanical device and pump to realize automatic liquid taking and adding operation in the extraction process. The existing automatic solid phase extraction device generally only can apply one sample pretreatment method flow at a time, and each extraction flow path is provided with one extraction column, so that a single solid phase extraction column can appear or the chemical separation and enrichment of target elements can not be completed by single solid phase extraction. Secondly, the number of the solution collecting pipe frames generally corresponds to the number of the sample pipes, and the fraction of a single sample cannot be collected for multiple times, so that the element composition change in the fraction is monitored.

Therefore, in order to solve the problems, the invention provides a double-mechanical arm type automatic solid phase extraction system and a use method thereof, wherein a solution collecting pipe frame with the number of 3 multiplied by 16 is arranged, the extraction fraction of a single sample can be collected for 16 times in a segmented way, and the change of element composition in the fraction is monitored and applied to the optimization work of the isotope sample pretreatment flow; the control system independently controls each component, and can simultaneously complete the operation of two different sample pretreatment flows; and the solution collected in the solution collecting pipe rack is sampled by controlling one group of sample feeding mechanical arms, and solid phase extraction is carried out again to replace a string column extraction mode, so that the dilemma that chemical separation and enrichment of target elements cannot be completed by single solid phase extraction column or single solid phase extraction is solved.

Disclosure of Invention

The invention provides a double-mechanical arm type automatic solid phase extraction system and a use method thereof, which can realize that a single extraction system can simultaneously carry out different chemical pretreatment processes and improve experimental efficiency.

In order to achieve the above object, the present invention provides the following solutions:

the automatic solid-phase extraction system with double mechanical arms comprises two groups of injection pump assemblies (1, 16), two groups of solution buffer pipes (2, 17), two groups of waste liquid tanks (15, 28) and a control system (29), wherein the two groups of injection needles (8, 22), the two groups of extraction columns (4, 19), the two groups of mechanical arms (5, 20), a transmission belt (6), the two groups of injection mechanical arm assemblies (7, 21), the two groups of injection needles (8, 22), the two groups of solution collecting mechanical arms (9, 23), the two groups of solution collecting mechanical arms (10, 24), the two groups of leaching reagent groups (11, 25), the two groups of waste liquid collecting bottles (12, 26), the two groups of sample pipe racks (13, 27), the solution collecting pipe racks (14), the two groups of waste liquid tanks (15, 28) and the control system (29) are used for combining the two groups of injection needles (8, 22), the solution collecting needles (10, 24) with the mechanical arms (5, 20), the two groups of injection mechanical arms (7, 21) and the solution collecting mechanical arms (9, 23) to form an automatic injection and solution collecting module, and independent solid-phase parts are respectively arranged on two sides of the two groups of the mechanical arms, and the independent solid-phase leaching components are respectively, the two groups of the leaching reagent groups are connected with the injection pump assemblies (3, the ten-phase pump assemblies (18, the ten-phase leaching reagent groups (1, the sample collecting valve assemblies and the waste liquid tank (11, the waste liquid collecting valve assemblies are connected with the injection pump assemblies (1, the waste liquid collecting valve assemblies respectively) 16 A solution buffer tube component (2, 17) is arranged between the ten-way valve component (3, 18) and the solution collecting needle (10, 24), and an extraction column component is arranged between the ten-way valve component and the solution collecting needle.

Preferably, the syringe pump assembly comprises at least 3 syringe pumps, and is connected with one end of the solution buffer tube assembly, and the other end of the solution buffer tube assembly is connected with the ten-way valve.

Preferably, the leaching reagent group comprises six leaching reagent pipes which are respectively communicated with a D interface to an I interface on the ten-way valve assembly.

Preferably, the port B of the ten-way valve is in communication with the 3X 16 digit solution collection tube.

Preferably, the port C of the ten-way valve is communicated with the waste liquid collecting bottle.

Preferably, the port A of the ten-way valve is communicated with the sample tube.

Preferably, said ten-way valve assembly comprises at least 3 of said ten-way valves.

The application method of the double-mechanical arm type automatic solid phase extraction device comprises the following steps:

under the instruction of a control system, each component ((No. 1 component and No. 2 component) on the left side and the right side of the mechanical arms (5 and 20) can independently complete the following steps in an asynchronous mode:

loading: transferring the digested sample solution into a centrifuge tube, placing the centrifuge tube on a sample tube rack (13, 27), driving a sample injection needle (8, 22) to reach the position of the designated sample tube rack (13, 27) by a sample injection mechanical arm (7, 21), quantitatively sucking the solution through a syringe pump assembly (1, 16) and controlling the flow rate of the solution in a pipeline, enabling the solution to enter a solution buffer tube assembly (2, 17) through an A port and a J port of a ten-way valve assembly (3, 18), reversing the ten-way valve assembly, switching to a B port, and pumping the solution in the buffer tube assembly out through the J port and the B port of the ten-way valve assembly by the syringe pump assembly to enter an extraction column (4, 19);

leaching and sample receiving: the ten-way valves (3, 18) are switched to any one of the interfaces from the interface D to the interface I, different leaching reagents (11, 25) are quantitatively extracted under the action of the injection pumps (1, 16) and sequentially pass through the extraction columns (4, 19), the extracted solution reaches the solution collecting sampling needles (10, 24) through the pipelines, the solution collecting sampling needles are controlled by the mechanical arms (5, 20) to collect the solution at the appointed positions on the 3 x 16-position solution collecting pipe frame (14), and 16 groups of solution fraction collection can be carried out in the single sample extraction process. The solution to be collected is not required, and is discharged from the solution collection needle (10, 24) to the position of the waste liquid tank (15, 28).

Cleaning an extraction column and a pipeline: after the rinsing process is finished, respectively quantitatively extracting different rinsing reagents (11, 25) sequentially pass through extraction columns (4, 19) under the action of injection pumps (1, 16) and ten-way valves (3, 18), and cleaning the balance extraction columns; then, the ten-way valves (3, 18) are switched to the interface A, the sampling needles (8, 22) move to the cleaning solution at specific positions on the sample tube frames (13, 27), the injection pumps (1, 16) are started to suck the cleaning solution, then the ten-way valves (3, 18) are switched to the interface C, and the injection pumps (1, 16) inject the cleaning waste liquid into the waste liquid collecting bottles (12, 26); after the completion, the directions of the ten-way valves (3, 18) are switched again, and under the instruction of the control system (29), the mechanical arms (5, 20) move the sampling needles (8, 22) to the appointed positions, and the next sampling is started.

Compared with the prior art, the invention has the following technical effects:

1. according to the invention, manual liquid adding operation is replaced, a plurality of extraction passages are arranged, so that the risk of cross contamination between samples is effectively reduced, the memory effect of a pipeline is reduced, and the adjustable extraction flow rate is realized through the cooperation of the closed solid-phase extraction column and the plunger pump.

2. The invention is provided with the solution collecting pipe frame with the number of 3 multiplied by 16, can collect the extraction fraction of a single sample for 16 times in a segmented way, monitors the change of element composition in the fraction, and is applied to the optimization work of the isotope sample pretreatment flow;

3. the invention adopts double mechanical arms type automatic sample injection and solution collection operation, and can simultaneously complete the operation of two different sample pretreatment processes; and the solution collected in the solution collecting tube rack is sampled by controlling one group of sample feeding mechanical arms, and solid phase extraction is performed again to replace a string column extraction mode, so that the dilemma that chemical separation and enrichment of target elements cannot be completed by a single solid phase extraction column or a single solid phase extraction is solved.

Drawings

In order to more clearly illustrate the invention or the technical solutions of the prior art, the drawings that are necessary for the embodiments will be briefly described below, it being evident that the drawings in the following description are only some embodiments of the invention and that other drawings can be obtained from these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic diagram of the structure of the present invention;

1. (1) a syringe pump assembly; 2. the solution buffer tube is No. 1; 3. (1) a ten-way valve assembly; 4. an extraction column (1); 5. a mechanical arm (1); 6. a transmission belt; 7. the sample injection mechanical arm assembly is the sample injection mechanical arm assembly (1); 8. a sample injection needle of the number (1); 9. a solution collection mechanical arm (1); 10. (1) a No. solution collection needle; 11. (1) rinsing the reagent; 12. a No. 1 waste liquid collecting bottle; 13. (1) sample tube rack; 14. 3X 16 digit solution collection tube rack; 15. a waste liquid tank No. 1; 16. (2) a syringe pump assembly; 17. a solution buffer tube No. 2; 18. (2) a ten-way valve assembly; 19. an extraction column (2); 20. a mechanical arm (2); 21. (2) a sample injection mechanical arm assembly; 22. (2) a sample injection needle; 23. a solution collection mechanical arm (2); 24. (2) a No. solution collection needle; 25. (2) a set of rinse reagents; 26. (2) a waste liquid collecting bottle; 27. (2) sample tube rack; 28. a waste liquid tank No. 2; 29. and a control system.

Detailed Description

The invention aims to provide a double-mechanical arm type automatic solid phase extraction system and a use method thereof, which achieve the aim of simultaneously running two different sample pretreatment flows.

In order that the above-recited objects, features and advantages of the present invention will become more readily apparent, a more particular description of the invention will be rendered by reference to the appended drawings and appended detailed description.

Referring to fig. 1, a dual-mechanical arm type automatic solid phase extraction system comprises two groups of injection pump assemblies, two groups of solution buffer tube assemblies, two groups of ten-way valve assemblies, two groups of extraction column assemblies, two groups of mechanical arms, a transmission belt, two groups of sample injection mechanical arm assemblies, two groups of sample injection needles, two groups of solution collection mechanical arms, two groups of solution collection needles, two groups of leaching reagent groups, two groups of waste liquid collection bottles, two groups of sample tube frames, a solution collection tube frame, two groups of waste liquid tanks and a control system. The components 1-15 form one set of extraction system, the components 16-28 form the other set of extraction system, the two sets of systems are independently controlled by the control system 29, and the sample injection needle 7 and the sample injection needle 21 not only can respectively absorb sample solution at the sample pipe rack 13 and the sample pipe rack 27 for extraction, but also can simultaneously operate two different sample pretreatment flows. The solution collecting pipe rack 14 is positioned between the mechanical arm 5 and the mechanical arm 20, and can be respectively driven by the mechanical arm 5 and the mechanical arm 20 to move to a designated position of the solution collecting pipe rack 14 to absorb the solution collected after extraction for sample injection again, so that continuous twice solid phase extraction of a single sample is completed, a string column extraction mode is replaced, and the dilemma that chemical separation and enrichment of target elements cannot be completed by single solid phase extraction column or single solid phase extraction is solved.

Referring to fig. 1, the syringe pump assembly includes at least 3 syringe pumps connected to one end of the solution buffer tube assembly, and the other end of the solution buffer tube assembly is connected to the ten-way valve.

Referring to fig. 1, the leaching reagent group comprises six leaching reagent pipes which are respectively communicated with a port number D to a port number I on the ten-way valve assembly.

Referring to fig. 1, the port B of the ten-way valve communicates with the extraction column assembly.

Referring to fig. 1, the port C of the ten-way valve communicates with the waste collection bottle.

Referring to fig. 1, the port a of the ten-way valve is in communication with a solution collection tube.

Referring to fig. 1, the ten-way valve assembly includes at least 3 of the ten-way valves.

Example 1:

first, an extraction column is activated: the ten-way valve assemblies 3 and 18 are switched to any one of the interfaces D to I, and different leaching reagents 11 and 25 are quantitatively extracted respectively under the action of the injection pump assemblies 1 and 16 and pass through the extraction columns 4 and 19 to finish activation.

Secondly, sample loading: transferring the digested sample solution into a centrifuge tube, respectively placing the centrifuge tube on a sample tube rack 13 and a sample tube rack 27, respectively driving a sample injection needle 7 and a sample injection needle 21 to reach the positions of the designated sample tube rack 13 and the sample tube rack 27 by a mechanical arm 5 and a mechanical arm 20, quantitatively sucking the solution through a syringe pump assembly 1 and a syringe pump assembly 16, controlling the flow rate of the solution in a pipeline, enabling the solution to enter a solution buffer tube assembly 2 and a solution buffer tube assembly 17 through an interface A and an interface J of a ten-way valve assembly, reversing the ten-way valve assembly, switching the ten-way valve assembly to an interface B, and pumping the solution in the solution buffer tube assembly out through the interface J and the interface B of the ten-way valve assembly by the syringe pump assembly to enter an extraction column 4 and a extraction column 19;

thirdly, leaching and sample receiving: the ten-way valves 3 and 18 are switched to any one of the interfaces from the interface D to the interface I, different leaching reagents 11 and 25 are quantitatively extracted under the action of the injection pumps 1 and 16, sequentially pass through the extraction columns 4 and 19, the extracted solution reaches the solution collecting sampling needles 10 and 24 through pipelines, the solution collecting sampling needles are controlled by the mechanical arms 5 and 20 to collect the solution at the designated positions on the solution collecting pipe frame 14, and the solution which is not required to be collected is moved to the positions of the waste liquid tanks 15 and 28 by the solution collecting needles 10 and 24 and is discharged.

Fourth, cleaning the extraction column and the pipeline: after the rinsing process is finished, respectively quantitatively extracting different rinsing reagents 11 and 25 sequentially pass through extraction columns 4 and 19 under the action of injection pumps 1 and 16 and ten-way valves 3 and 18, and cleaning the balance extraction columns; then, the ten-way valves 3 and 18 are switched to the interface A, the sample injection needles 8 and 22 move to the cleaning solution at specific positions on the sample tube frames 13 and 27, the injection pumps 1 and 16 are started to suck the cleaning solution, then the ten-way valves 3 and 18 are switched to the interface C, and the injection pumps 1 and 16 inject the cleaning waste liquid into the waste liquid collecting bottles 12 and 26; after the completion, the directions of the ten-way valves 3 and 18 are switched again, and under the instruction of the control system 29, the mechanical arms 5 and 20 move the sample injection needles 8 and 22 to the designated positions, and the next sample loading is started.

Example 2:

first, an extraction column is activated: the procedure was the same as in example 1.

Secondly, loading the sample on an extraction column assembly No. 4: transferring the digested sample solution to a centrifuge tube, respectively placing the sample solution on a sample tube rack 13 (1), driving a sample injection needle 7 (1) to reach a designated position of the sample tube rack 13 (1) by a mechanical arm 5 (1), quantitatively sucking the solution through a syringe pump assembly 1 (1), leading the solution to enter a solution buffer tube assembly 2 (1) through an interface A and an interface J of a ten-way valve assembly 3 (1), reversing the valve assembly 3 (1), switching to an interface B, and leading the solution in the solution buffer tube assembly (1) to be pumped out through the interface J and the interface B of the ten-way valve assembly 3 (1) by the syringe pump assembly 1, and leading the solution to enter an extraction column 4 (1);

thirdly, leaching and sample receiving of the extraction column assembly No. 4: (1) the ten-way valve assembly 3 is switched to any one of the interfaces from the interface D to the interface I, different (1) eluting reagent sets 11 are quantitatively extracted under the action of the (1) injection pump assembly 1, sequentially pass through the (1) extraction column assembly 4, the extracted solution reaches the (1) solution collecting sample injection needle 10 through a pipeline, the solution collecting sample injection needle is controlled by the (1) mechanical arm 5 to collect the solution at the appointed position on the solution collecting pipe rack 14, and the solution which is not required to be collected is removed from the (1) solution collecting needle 10 to the position of the (1) waste liquid tank 15 to be discharged.

Fourth step, (2) loading the extraction column assembly 19: (2) the mechanical arm 20 drives the sample injection needle 21 (2) to reach the designated position of the solution collecting pipe rack 14 (2), the solution is quantitatively sucked through the injection pump assembly 16 (2), the solution enters the solution buffer pipe assembly 17 (2) through the interface A and the interface J of the ten-way valve assembly 18 (2), the ten-way valve assembly 18 is reversed, the solution is switched to the interface B, and the injection pump assembly 16 (2) pumps the solution in the solution buffer pipe assembly (2) through the interface J and the interface B of the ten-way valve assembly 18 (2) and enters the extraction column 19 (2);

fifth step, (2) No. 19 leaching and sample receiving: (2) the ten-way valve assembly 18 is switched to any one of the D-port and the I-port, different (2) -number leaching reagent sets 25 are quantitatively extracted under the action of the (2) -number injection pump assembly 16 and sequentially pass through the (2) -number extraction column assembly 19, the extracted solution reaches the (2) -number solution collecting sample injection needle 24 through a pipeline, the (2) -number mechanical arm 20 controls the solution sample injection needle to collect the solution at a designated position on the solution collecting pipe rack 14, and the solution which is not required to be collected is moved to the (2) -number waste liquid tank 28 by the (2) -number solution collecting needle 24 to be discharged.

Sixth step, cleaning the extraction columns 4 and 19 and the pipelines: the procedure is the same as in the fourth step of example 1.

The adaptation according to the actual requirement includes not only the system material, the number of extraction modules, etc. but also the protection scope of the present invention.

It should be noted that it will be apparent to those skilled in the art that the present invention is not limited to the details of the above-described exemplary embodiments, but may be embodied in other specific forms without departing from the spirit or essential characteristics thereof. The present embodiments are, therefore, to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims (9)

1. The double-mechanical-arm type automatic solid-phase extraction system is characterized by comprising two groups of injection pump assemblies (1 and 16), two groups of solution buffer pipes (2 and 17), two groups of waste liquid collecting bottles (12 and 26), two groups of waste liquid tanks (15 and 28) and a control system (29), wherein the two groups of injection needles (8 and 22), the solution collecting needles (10 and 24) and the mechanical arms (5 and 20), the transmission belts (6), the two groups of injection mechanical arm assemblies (7 and 21), the two groups of injection needles (8 and 22), the two groups of solution collecting mechanical arms (9 and 23), the two groups of solution collecting mechanical arms (10 and 24), the two groups of leaching reagent groups (11 and 25), the two groups of waste liquid collecting bottles (12 and 26), the two groups of sample pipe racks (13 and 27), the 3X 16-bit solution collecting pipe racks (14), the two groups of waste liquid tanks (15 and 28) and the control system (29), the two groups of injection needles (8 and 22), the solution collecting needles (10 and 24) and the mechanical arms (5 and 20), the injection mechanical arms (7 and 21), the solution collecting mechanical arms (9 and 23) are combined to form a double-mechanical-arm type automatic sample collecting module, and a leaching reagent collecting module, and the leaching reagent collecting module is arranged on two sides of the two mechanical arms, and one group of the independent injection needle assemblies is provided with the ten-phase connecting valve assemblies (3 and the two groups of waste liquid collecting tube (3 and the waste liquid collecting tube (3 and 16) respectively, the solid-phase extracting device (1 and the solid-phase extraction system 18 The device comprises a ten-way valve, a solution buffer tube assembly (2, 17) is arranged between a J-shaped interface of the ten-way valve and the injection pump (1, 16), and an extraction column assembly is arranged between the ten-way valve assembly (3, 18) and the solution collecting needle (10, 24).

2. The automated solid phase extraction system of claim 1, wherein the syringe pump assembly comprises at least 3 syringes connected to one end of the solution buffer tube assembly, and the other end of the solution buffer tube assembly is connected to the ten-way valve.

3. The automated solid phase extraction system of claim 1, wherein the leaching reagent set comprises six leaching reagent tubes respectively communicating with the D-port to I-port on the ten-way valve assembly.

4. A dual-robotic automated solid phase extraction system according to claim 3, wherein the ten-way valve port B is in communication with the 3 x 16-digit solution collection tube.

5. The automated solid phase extraction system of claim 4, wherein the ten-way valve port C is in communication with the waste collection bottle.

6. The automated solid phase extraction system of claim 4, wherein the ten-way valve port a is in communication with the sample tube.

7. The dual robotic arm automated solid phase extraction system of claim 1, wherein the ten-way valve assembly comprises at least 3 of the ten-way valves.

8. The automated solid phase extraction system of claim 1, wherein the syringe pump, the solution buffer tube, the extraction column, the solution line, the sample injection needle, the solution collection needle, and the ten-way valve assembly are all made of corrosion resistant materials.

9. The application method of the double-mechanical-arm type automatic solid-phase extraction device is characterized by comprising the following steps of:

under the instruction of a control system, the mechanical arms (5, 20) and the components on two sides thereof form two sets of single-arm automatic solid-phase extraction systems, and the following steps can be respectively completed in a synchronous or asynchronous mode:

loading: transferring the digested sample solution into a centrifuge tube, placing the centrifuge tube on a sample tube rack (13, 27), driving a sample injection needle (8, 22) to reach the position of the designated sample tube rack (13, 27) by a sample injection mechanical arm (7, 21), quantitatively sucking the solution through a syringe pump assembly (1, 16) and controlling the flow rate of the solution in a pipeline, enabling the solution to enter a solution buffer tube assembly (2, 17) through an A port and a J port of a ten-way valve assembly (3, 18), reversing the ten-way valve assembly, switching to a B port, and pumping the solution in the buffer tube assembly out through the J port and the B port of the ten-way valve assembly by the syringe pump assembly to enter an extraction column (4, 19);

leaching and sample receiving: reversing a ten-way valve (3, 18), switching to any one of a D interface and an I interface, quantitatively extracting different leaching reagents (11, 25) under the action of an injection pump (1, 16), sequentially passing through an extraction column (4, 19), enabling the extracted solution to reach a solution collecting sample injection needle (10, 24) through a pipeline, controlling the solution sample injection needle to collect the solution at a designated position on a solution collecting pipe frame (14) with the number of 3 multiplied by 16 by a mechanical arm (5, 20), collecting 16 groups of solution fractions in the single sample extraction process, monitoring the change of element compositions in the fractions, and applying the method to the optimization work of an isotope sample pretreatment flow; the solution to be collected is not required, and is discharged from the solution collection needle (10, 24) to the position of the waste liquid tank (15, 28).

Cleaning an extraction column and a pipeline: after the rinsing process is finished, respectively quantitatively extracting different rinsing reagents (11, 25) sequentially pass through extraction columns (4, 19) under the action of injection pumps (1, 16) and ten-way valves (3, 18), and cleaning the balance extraction columns; then, the ten-way valves (3, 18) are switched to the interface A, the sampling needles (8, 22) move to the cleaning solution at specific positions on the sample tube frames (13, 27), the injection pumps (1, 16) are started to suck the cleaning solution, then the ten-way valves (3, 18) are switched to the interface C, and the injection pumps (1, 16) inject the cleaning waste liquid into the waste liquid collecting bottles (12, 26); after the completion, the directions of the ten-way valves (3, 18) are switched again, and under the instruction of the control system (29), the mechanical arms (5, 20) move the sampling needles (8, 22) to the appointed positions, and the next sampling is started.