CN112798389A - Radionuclide solid phase extraction system - Google Patents
Radionuclide solid phase extraction system Download PDFInfo
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- CN112798389A CN112798389A CN202011616988.6A CN202011616988A CN112798389A CN 112798389 A CN112798389 A CN 112798389A CN 202011616988 A CN202011616988 A CN 202011616988A CN 112798389 A CN112798389 A CN 112798389A
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- solid phase
- phase extraction
- extraction system
- radionuclide
- extraction column
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/34—Purifying; Cleaning
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/40—Concentrating samples
- G01N1/405—Concentrating samples by adsorption or absorption
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- Molecular Biology (AREA)
- Engineering & Computer Science (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a radionuclide solid phase extraction system, which comprises: a sample pipe frame; collecting tank; collecting the pipe frame; a mechanical arm; the insertion pipe is mounted on the mechanical arm; an extraction column; a power plant; and a controller. The controller controls the mechanical arm to drive the insertion pipe to move, the extraction column is moved to the position above the collecting tank or the position above the collecting pipe frame through the insertion pipe, and washing buffer solution, elution buffer solution or liquid samples from the sample pipe frame which are driven and input by the power device are injected into the extraction column through the insertion pipe. The scheme of the invention can automatically complete the separation and purification process in the radionuclide pretreatment, not only can reduce the labor intensity of operators, save the analysis time and improve the sample treatment flux, but also can greatly improve the accuracy and the repetition rate of sample analysis, and can avoid the damage that the operators must endure various high-concentration acid solutions and acid mists for a long time.
Description
Technical Field
The invention relates to the technical field of radioactivity detection, in particular to a radionuclide solid-phase extraction system.
Background
In the analysis methods of radioactive nuclides of plutonium, uranium, strontium, neptunium, americium and the like in water, environment and biological samples, the steps of separation, concentration and purification of the radioactive nuclides are required. The measurement of the radioactive nuclide can be roughly divided into three processes of pretreatment, separation and purification and measurement, and the separation and purification process in the pretreatment of the radioactive nuclide adopts a manual solid-phase extraction device in most laboratories. However, manual solid phase extraction is difficult to achieve with high reproducibility, is inefficient, and suffers from various high concentrations of acid solutions and acid mists that operators tolerate for long periods of time.
Disclosure of Invention
It is a primary object of the present invention to provide a radionuclide solid phase extraction system that overcomes at least one of the above-mentioned technical problems.
In order to achieve the above object, the present invention provides a radionuclide solid-phase extraction system comprising: a sample pipe frame; collecting tank; collecting the pipe frame; a mechanical arm; an insertion tube mounted on the robotic arm; an extraction column; a power plant; and a controller. The controller controls the mechanical arm to drive the insertion pipe to move, the extraction column is moved to the position above the collecting tank or the position above the collecting pipe frame through the insertion pipe, and washing buffer solution, elution buffer solution or liquid samples from the sample pipe frame which are driven and input by the power device are injected into the extraction column through the insertion pipe.
Further, the extraction column is filled with a solid adsorbent; the first end of the extraction column is sealed by a first sealing element, and the second end of the extraction column is sealed by a second sealing element.
Further, the first sealing element adopts a disposable resin column sealing cover; the second sealing element is covered by a disposable resin column.
Further, the power device comprises a syringe pump.
Further, the radionuclide solid phase extraction system also comprises a gun head frame; the gun head frame comprises a plurality of accommodating holes and a plurality of gun heads placed in the accommodating holes; the cannula may be impaled into the lance tip.
Further, the lance head is a disposable lance head.
Further, the radionuclide solid phase extraction system also comprises a waste gunhead box; wherein the waste gun head box is used for collecting used disposable gun heads.
Further, the radionuclide solid phase extraction system further comprises a rack; wherein, rifle headstock, sample pipe support, collecting vat, collection pipe support, abandonment rifle head box, arm, intubate, extraction post, power device and controller all set up in the frame.
Further, the gun head frame, the sample pipe frame, the collecting tank and the waste gun head box are sequentially arranged on the rack along a first direction.
Further, the controller controls the mechanical arm to drive the insertion tube to move along the first direction, or controls the mechanical arm to drive the insertion tube to move along the second direction.
Further, the second direction is perpendicular to the first direction.
Further, the controller adopts an industrial personal computer or a PC.
Further, the extraction column is arranged on the rack through an extraction column frame.
Further, the cannula is connected to the power unit through a piping system and a flow path control unit.
As described above, the present invention provides a radionuclide solid phase extraction system, which can control a mechanical arm to drive an insertion tube to move through a controller, further move an extraction column to a position above a collection tank or a position above a collection pipe frame through the insertion tube, and inject liquid into the extraction column through the insertion tube, such as a liquid sample from a sample pipe frame, a washing buffer solution or an elution buffer solution, which is driven and input by a power device, so as to automatically complete a separation and purification process in a radionuclide pretreatment process.
Drawings
Other objects and advantages of the present invention will become apparent from the following description of the invention which refers to the accompanying drawings, and may assist in a comprehensive understanding of the invention.
FIG. 1 is a schematic diagram of the construction of a radionuclide solid phase extraction system according to some embodiments of the invention;
FIG. 2 is an enlarged schematic view of a central subassembly of the solid phase extraction system for radionuclides illustrated in FIG. 1;
fig. 3 is a schematic diagram of the connection of a cannula to an extraction column in a radionuclide solid phase extraction system according to some embodiments of the invention.
It is noted that the drawings are not necessarily to scale and are merely illustrative in nature and not intended to obscure the reader.
Description of reference numerals:
100. a radionuclide solid phase extraction system; 110. a frame; 120. a headstock; 121. a housing hole; 122. a gun head; 130. a sample pipe frame; 140. collecting tank; 150. collecting the pipe frame; 160. discarding the gun head box; 170. a mechanical arm; 176. inserting a tube; 180. an extraction column; 182. a first seal member; 184. a second seal member; 186. an extraction column frame; 190. and a power device.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described clearly and completely with reference to the accompanying drawings of the embodiments of the present invention. It should be apparent that the described embodiment is one embodiment of the invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the described embodiments of the invention without any inventive step, are within the scope of protection of the invention.
It is to be noted that technical terms or scientific terms used herein should have the ordinary meaning as understood by those having ordinary skill in the art to which the present invention belongs, unless otherwise defined. If the description "first", "second", etc. is referred to throughout, the description of "first", "second", etc. is used only for distinguishing similar objects, and is not to be construed as indicating or implying a relative importance, order or number of technical features indicated, it being understood that the data described in "first", "second", etc. may be interchanged where appropriate. If "and/or" is presented throughout, it is meant to include three juxtapositions, exemplified by "A and/or B" and including either scheme A, or scheme B, or schemes in which both A and B are satisfied. Furthermore, spatially relative terms, such as "above," "below," "top," "bottom," and the like, may be used herein for ease of description to describe one element or feature's spatial relationship to another element or feature as illustrated in the figures, and should be understood to encompass different orientations in use or operation in addition to the orientation depicted in the figures.
Solid phase extraction is a sample separation and enrichment technology, and is developed by combining liquid-solid phase extraction and column liquid chromatography technology. The solid phase extraction is mainly used for separating and enriching micro or trace components in a complex sample. Specifically, the liquid sample passes through a solid phase extraction device filled with a solid adsorbent under the action of positive pressure, negative pressure or gravity, so that the purification and separation of the target compound are completed.
Some embodiments of the present invention provide a radionuclide solid-phase extraction system, which can automatically complete the separation and purification process in the radionuclide pretreatment, not only reduce the labor intensity of operators, save the analysis time, and improve the sample processing throughput, but also greatly improve the accuracy and the repetition rate of sample analysis, and avoid the damage that the operators must endure various high-concentration acid solutions and acid mists for a long time.
In particular, fig. 1 is a schematic structural diagram of a radionuclide solid phase extraction system according to some embodiments of the invention; FIG. 2 is an enlarged schematic view of the central subassembly of the solid phase extraction system for radionuclides shown in FIG. 1. As shown in fig. 1 and 2, the radionuclide solid phase extraction system 100 includes a rack 110, a gun head rack 120, a sample pipe rack 130, a collection tank 140, a collection pipe rack 150, a waste gun head box 160, a robot arm 170, a cannula 176, an extraction column 180, a power unit 190, and a controller (not shown). The gun head holder 120, the sample tube holder 130, the collection tank 140, the collection tube holder 150, the waste gun head box 160, the mechanical arm 170, the insertion tube 176, the extraction column 180, the power device 190 and the controller are all arranged in the rack 110, the controller controls the mechanical arm 170 to drive the insertion tube 176 to move, the extraction column 180 is moved to the position above the collection tank 140 or the position above the collection tube holder 150 through the insertion tube 176, and liquid is injected into the extraction column 180 through the insertion tube 176, for example, liquid samples, washing buffer solutions or elution buffer solutions and the like from the sample tube holder 130 are driven and input by the power device 190.
Specifically, in the embodiment shown in fig. 2, the gun head rack 120, the sample rack 130, the collection tank 140, the collection rack 150, and the waste gun head box 160 are sequentially disposed on the rack 110 along the first direction D1.
Wherein the lance tip holder 120 is disposed on the frame 110. The gun head frame 120 includes a plurality of receiving holes 121 and a plurality of gun heads 122 placed in the plurality of receiving holes 121. The lance tip 122 may be a disposable lance tip. In one embodiment, the lance tip 122 may be a 10mL disposable lance tip.
The collecting tank 140 is used for collecting waste liquid generated in the separation and purification process of radionuclide pretreatment.
The collection tube holder 150 is used to place an elution tube for collecting the eluate.
The waste tip magazine 160 is used to collect the used disposable tips 122.
The controller may control the robotic arm 170 to move the cannula 176 in the first direction D1, and the controller may control the robotic arm 170 to move the cannula 176 in the second direction D2. The second direction D2 is substantially perpendicular to the first direction D1. In one embodiment, the controller may be an industrial personal computer or a PC.
The cannula 176 is mounted on the robotic arm 170 and is connected to the power unit 190 through tubing and a flow path control unit. The flow path control unit is responsible for switching flow paths to ensure that different solvents are automatically connected into the pipeline system, and the solvents in the pipeline system can flow into the insertion pipe 176 under the driving of the power device 190.
The power unit 190 is used to power an input liquid such as a solvent, control flow rate, and solvent volume. In one embodiment, the power unit 190 employs a syringe pump.
The extraction column 180 may be disposed on the housing 110 through an extraction column holder 186. The extraction column 180 is filled with a solid adsorbent such as resin. As shown in fig. 3, a first end of the extraction column 180 is sealed by a first seal 182 and a second end of the extraction column 180 is sealed by a second seal 184. By providing the first and second seals 182, 184, the extraction column is guaranteed to be wet throughout. In one embodiment, the first sealing member 182 may be a disposable resin post sealing cap, and the second sealing member 184 may also be a disposable resin post sealing cap. During the loading, rinsing, and elution steps of the separation and purification process of radionuclide pretreatment, the cannula 176 can pass through the first seal 182 and seal the first end of the extraction column 180 together with the first seal 182.
The above is a specific structure of the radionuclide solid phase extraction system 100 in an embodiment of the present invention, and the following is a brief description of a specific method for using the radionuclide solid phase extraction system in the steps of loading, rinsing and eluting during the separation and purification process.
A sample loading step: the controller controls the mechanical arm 170 to drive the cannula 176 to move above the gun head holder 120, and controls the cannula 176 to move towards the gun head holder 120 along the second direction D2, so that the cannula 176 automatically sticks 10mL of disposable gun head 122; then, the controller controls the mechanical arm 170 to drive the insertion tube 176 to move the extraction column 180 until the extraction column 180 is located right above the collection tank 140; the controller controls the mechanical arm 170 to drive the insertion tube 176 to move above the sample tube rack 130, and controls the insertion tube 176 to move towards the sample tube rack 130 along the second direction D2, so that the insertion tube 176 extends into a sample tube in the sample tube rack 130 and performs liquid suction through a power device 190, such as a syringe pump, and further sucks a sample in the sample tube into a 10mL disposable tip 122; thereafter, the controller controls the mechanical arm 170 to move the cannula 176 over the extraction column 180 and controls the cannula 176 to move in the second direction D2 toward the extraction column 180, so that the cannula 176 passes through the first sealing member 182 for loading.
After the sample loading is completed, the controller controls the mechanical arm 170 to drive the insertion tube 176 to move to the upper part of the waste gun head box 160 to throw the gun head 122.
Leaching: the controller controls the mechanical arm 170 to drive the cannula 176 to move above the gun head holder 120, and controls the cannula 176 to move towards the gun head holder 120 along the second direction D2, so that the cannula 176 automatically sticks 10mL of disposable gun head 122; then, the controller controls the mechanical arm 170 to drive the insertion tube 176 to move the extraction column 180 until the extraction column 180 is located right above the collection tank 140; the controller controls the insertion tube 176 to move in the second direction D2 toward the extraction column 180 such that the insertion tube 176 passes through the first sealing member 182 and draws a corresponding fluid through the power unit 190, such as a syringe pump, to rinse the extraction column 180.
After the rinsing is completed, the controller controls the mechanical arm 170 to drive the insertion tube 176 to move to the upper part of the waste gun head box 160 to perform the gun head 122 throwing.
An elution step: the controller controls the mechanical arm 170 to drive the cannula 176 to move above the gun head holder 120, and controls the cannula 176 to move towards the gun head holder 120 along the second direction D2, so that the cannula 176 automatically sticks 10mL of disposable gun head 122; then, the controller controls the mechanical arm 170 to drive the insertion tube 176 to move the extraction column 180 until the extraction column 180 is positioned right above the collection tube rack 150; the extraction column 180 is eluted by drawing in a corresponding liquid through a power unit 190, such as a syringe pump, so that the eluate is collected in an elution tube located in the collection tube holder 150.
After the elution is completed, the controller controls the mechanical arm 170 to drive the insertion tube 176 to move to the upper part of the waste gun head box 160 to perform the gun head 122 throwing.
As described above, some embodiments of the present invention provide a radionuclide solid phase extraction system 100, which can control a robot arm 170 to drive an insertion tube 176 to move through a controller, and then move an extraction column 180 above a collection tank 140 or above a collection tube rack 150 through the insertion tube 176, and inject liquid, such as a liquid sample from a sample tube rack 130, a washing buffer, an elution buffer, etc., which are driven by a power device 190 into the extraction column 180 through the insertion tube 176, so as to automatically complete a separation and purification process in a radionuclide pretreatment process, thereby reducing the labor intensity of an operator, saving the analysis time, improving the sample processing throughput, greatly improving the accuracy and the repetition rate of the sample analysis, and avoiding the operator from being damaged by various high-concentration acid solutions and acid mists for a long time.
It should also be noted that, in the case of the embodiments of the present invention, features of the embodiments and examples may be combined with each other to obtain a new embodiment without conflict.
The above description is only an embodiment of the present invention, but the scope of the present invention is not limited thereto, and the scope of the present invention is subject to the scope of the claims.
Claims (14)
1. A radionuclide solid phase extraction system (100) comprising:
a sample tube rack (130);
a collection tank (140);
a collection tube rack (150);
a robotic arm (170);
a cannula (176), the cannula (176) mounted on the robotic arm (170);
an extraction column (180);
a power plant (190);
a controller;
the controller controls the mechanical arm (170) to drive the insertion pipe (176) to move, so that the extraction column (180) is moved to the position above the collecting tank (140) or the position above the collecting pipe rack (150) through the insertion pipe (176), and washing buffer, elution buffer or liquid samples from the sample pipe rack (130) driven and input by the power device (190) are injected into the extraction column (180) through the insertion pipe (176).
2. The radionuclide solid phase extraction system (100) according to claim 1, characterized in that: the extraction column (180) is filled with a solid adsorbent; the first end of the extraction column (180) is sealed by a first seal (182) and the second end of the extraction column (180) is sealed by a second seal (184).
3. The radionuclide solid phase extraction system (100) according to claim 2, characterized in that: the first sealing element (182) adopts a disposable resin column sealing cover; the second seal (184) is capped with a disposable resin post.
4. The radionuclide solid phase extraction system (100) according to claim 3, characterized in that: the power device (190) comprises a syringe pump.
5. The radionuclide solid phase extraction system (100) according to claim 4, characterized in that: also includes a headstock (120); wherein the headstock (120) comprises a plurality of housing holes (121) and a plurality of lances (122) placed in the plurality of housing holes (121); the cannula (176) may be inserted into the lance tip (122).
6. The radionuclide solid phase extraction system (100) according to claim 5, characterized in that: the lance tip (122) is a disposable lance tip.
7. The radionuclide solid phase extraction system (100) according to claim 6, characterized in that: also includes a waste cartridge (160); wherein the waste tip magazine (160) is used for collecting used disposable tips (122).
8. The radionuclide solid phase extraction system (100) according to claim 7, characterized in that: further comprising a frame (110); the gun head frame (120), the sample pipe frame (130), the collecting groove (140), the collecting pipe frame (150), the waste gun head box (160), the mechanical arm (170), the insertion pipe (176), the extraction column (180), the power device (190) and the controller are all arranged in the rack (110).
9. The radionuclide solid phase extraction system (100) according to claim 8, characterized in that: the gun head frame (120), the sample pipe frame (130), the collection pipe frame (150), the collection groove (140) and the waste gun head box (160) are sequentially arranged on the rack (110) along a first direction.
10. The radionuclide solid phase extraction system (100) according to claim 9, characterized in that: the controller controls the mechanical arm (170) to drive the insertion tube (176) to move along the first direction, or controls the mechanical arm (170) to drive the insertion tube (176) to move along the second direction.
11. The radionuclide solid phase extraction system (100) according to claim 10, characterized in that: the second direction is perpendicular to the first direction.
12. The radionuclide solid phase extraction system (100) according to claim 10, characterized in that: the controller adopts an industrial personal computer or a PC.
13. The radionuclide solid phase extraction system (100) according to claim 10, characterized in that: the extraction column (180) is disposed on the rack (110) through an extraction column holder (186).
14. The radionuclide solid phase extraction system (100) according to any of claims 1 to 13, characterized in that: the cannula (176) is connected to the power unit (190) via a tubing system and a flow path control unit.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN202011616988.6A CN112798389A (en) | 2020-12-30 | 2020-12-30 | Radionuclide solid phase extraction system |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202011616988.6A CN112798389A (en) | 2020-12-30 | 2020-12-30 | Radionuclide solid phase extraction system |
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| CN112798389A true CN112798389A (en) | 2021-05-14 |
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| CN202011616988.6A Pending CN112798389A (en) | 2020-12-30 | 2020-12-30 | Radionuclide solid phase extraction system |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113390702A (en) * | 2021-05-31 | 2021-09-14 | 烟台海关技术中心 | Radioactive strontium solid-phase extraction, sample preparation and activity measurement integrated device |
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| CN113390702A (en) * | 2021-05-31 | 2021-09-14 | 烟台海关技术中心 | Radioactive strontium solid-phase extraction, sample preparation and activity measurement integrated device |
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