CN117084245A - 一种含白藜芦醇的农药组合物 - Google Patents
一种含白藜芦醇的农药组合物 Download PDFInfo
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- CN117084245A CN117084245A CN202311071956.6A CN202311071956A CN117084245A CN 117084245 A CN117084245 A CN 117084245A CN 202311071956 A CN202311071956 A CN 202311071956A CN 117084245 A CN117084245 A CN 117084245A
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- 239000000575 pesticide Substances 0.000 title claims abstract description 19
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- WYXXLXHHWYNKJF-UHFFFAOYSA-N isocarvacrol Natural products CC(C)C1=CC=C(O)C(C)=C1 WYXXLXHHWYNKJF-UHFFFAOYSA-N 0.000 description 1
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/08—Oxygen or sulfur directly attached to an aromatic ring system
- A01N31/16—Oxygen or sulfur directly attached to an aromatic ring system with two or more oxygen or sulfur atoms directly attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N41/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom
- A01N41/02—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a sulfur atom bound to a hetero atom containing a sulfur-to-oxygen double bond
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明属于农药技术领域，具体涉及一种含白藜芦醇的农药组合物。一种含白藜芦醇的农药组合物，其有效成分由蛇床子素或大蒜素与白藜芦醇复配而成。本发明将蛇床子素或大蒜素与与白藜芦醇以一定质量进行复配，对多种植物真菌性病害病原菌表现出协同增效作用，可以提高对植物病害的防治效果，为小麦茎基腐病、小麦赤霉病、花生根腐病、花生白绢病及玉米赤霉病的有效防控提供了数据支撑，且对小麦、花生、玉米等作物的优质高产具有重要的意义。
Description
技术领域
本发明属于农药技术领域，具体涉及一种含白藜芦醇的农药组合物。
背景技术
植物源农药是指从植物中提取具有杀虫或抗菌作用的活性物质，直接或间接加工合成的新型农药，其涵盖了从植物中提纯的活性成分、植物本身和对活性结构修饰后合成的化合物。植物源农药具有环保、生物活性多样、有害生物不易产生抗药性和对非靶标生物安全等优点而备受关注和青睐。目前，植物源农药产品已有很多在生产上被登记用于有害生物的防控，如苦参碱、茶蝉净、藜芦碱等。
白藜芦醇(Resveratrol)又称芪三芬，分子式为C14H12O3，是一种天然存在于葡萄、花生、蓝莓、虎杖等多种植物中的非黄酮类天然多酚化合物，具有抗氧化、抗菌消炎、抗心血管疾病、抗肿瘤等多种生物活性。白藜芦醇具有顺式和反式两种异构体，通常在自然界中以反式构型为主。白藜芦醇作为一种植物抗毒素，在植物中发挥着保护作用，具有抵御紫外线辐射、预防真菌感染等作用。
已有研究表明，白藜芦醇因在促进人体营养健康中发挥了抗氧化、抗菌、抗炎症、抗肿瘤、抗心血管等优异的功能活性，在食品、药品及日化品领域都有广泛的应用。已有研究表明，白藜芦醇课通过抑制糖酵解的方式来抑制哺乳动物肝细胞的增殖，肝细胞对白藜芦醇也较为敏感。同时，Parekh等发现白藜芦醇通过促进细胞凋亡来抑制人HepG2肝癌细胞的生长。Liu等发现白藜芦醇联合顺铂可通过降低ASCT2的表达来减少谷氨酰胺的吸收，并提高顺铂的抗肿瘤活性。此外，Dai等发现白藜芦醇通过March1诱导的PTEN/Akt信号转导调节抑制肝细胞癌的恶性进展。研究结果进一步表明了白藜芦醇可通过诱导癌细胞凋亡、抑制癌细胞生长、加强化疗药物活性等作用来抑制哺乳动物癌细胞的扩展。
当前，关于白藜芦醇的研究涉及到植物病害方面的很少，本课题组研究发现白藜芦醇对禾谷镰刀菌和假禾谷镰刀菌具有优异的抑菌活性，对齐整小核菌、立枯丝核菌、变红镰刀菌、木贼镰刀菌、层出镰刀菌和尖孢镰刀菌具有较强的抑菌活性，在防控小麦赤霉病、小麦茎基腐病、玉米赤霉病和花生根腐病方面具有较强的潜力。
为了进一步扩大防治谱和提高对植物病害的防治效果，本发明进一步开展了白藜芦醇与候选植物源杀菌剂厚朴酚、香芹酚、牛至油、蛇床子素或大蒜素二元复配研究，结果表明白藜芦醇与候选杀菌剂复配时对禾谷镰刀菌、假禾谷镰刀菌、齐整小核菌、立枯丝核菌、变红镰刀菌、木贼镰刀菌、层出镰刀菌、尖孢镰刀菌表现出了较强的增效作用，进一步表明了白藜芦醇及其复配组合对小麦赤霉病、小麦茎基腐病、玉米赤霉病和花生根腐病具有较强的防控潜力，本发明对有效防控小麦赤霉病、小麦茎基腐病、玉米赤霉病和花生根腐病，进一步确保小麦、玉米及花生安全生产具有重要的意义。
公开于该背景技术部分的信息仅仅旨在增加对本发明的总体背景的理解，而不应当被视为承认或以任何形式暗示该信息构成已为本领域一般技术人员所公知的现有技术。
发明内容
本发明的目的在于提供一种含白藜芦醇的农药组合物，其可以有效抑制小麦茎基腐病、小麦赤霉病、花生根腐病、花生白绢病和玉米赤霉病等病原菌的生长，有效防控多种植物真菌性病害，对小麦、花生、玉米等作物的优质高产具有重要的意义。
为实现上述目的，本发明提供了如下技术方案：
一种含白藜芦醇的农药组合物，其有效成分由蛇床子素或大蒜素与白藜芦醇复配而成。
作为优选，所述蛇床子素与白藜芦醇的质量比为1-9：9-1。
作为优选，所述大蒜素与白藜芦醇的质量比为1-9：9-1。
本发明还提供了所述的含白藜芦醇的农药组合物在防治农作物真菌性病害中的用途。
作为优选，所述农作物真菌性病害包括小麦茎基腐病、小麦赤霉病、花生根腐病、花生白绢病和玉米赤霉病。
与现有技术相比，本发明具有如下有益效果：
本发明将蛇床子素或大蒜素与白藜芦醇以一定质量进行复配，对多种植物真菌性病害病原菌表现出协同增效作用，可以提高对植物病害的防治效果，为小麦茎基腐病、小麦赤霉病、花生根腐病、花生白绢病及玉米赤霉病的有效防控提供了数据支撑，且对小麦、花生、玉米等作物的优质高产具有重要的意义。
具体实施方式
下面结合对本发明专利的技术方案进行清楚、完整的描述。
实施例
1.供试菌株
表1供试菌株
2.供试药剂
99％蛇床子素原药(上海阿拉丁生化科技股份有限公司)、98％大蒜素原药(上海源叶生物科技有限公司)、98％白藜芦醇原药(上海源叶生物科技有限公司)。
供试药剂溶解后用0.1％吐温-80水溶液稀释成单剂母液，设置多组配比，各单剂母液和配比混剂均按等比方法设置5个质量浓度梯度。
3.试验方法
将预先融化的PDA培养基9mL加入无菌锥形瓶中，从低浓度到高浓度依次定量吸取药液1mL，分别加入上述锥形瓶中，充分摇匀后倒入1个直径为9cm的培养皿中，制成相应浓度的含药平板，同时设不含药剂的处理作空白对照，每个质量浓度药液处理10个平板。用直径5mm打孔器在供试菌株菌落边缘切取菌饼，接种于含药平板和空白对照平板中央，盖上皿盖后置于25℃恒温箱培养。待空白对照菌落直径长至培养皿直径的2/3左右时，用十字交叉法测量菌落直径，计算不同处理对菌丝生长抑制率。
4.数据分析
采用DPS软件进行数据统计分析，得出毒力回归方程及药剂对靶标病菌的毒力EC50值，并根据孙云沛法计算共毒系数(CTC)。
5.测定结果
根据计算的共毒系数(CTC)评价药剂的增效作用，CTC≤80为拮抗作用，80＜CTC＜120为相加作用，CTC≥120为增效作用，结果见表2-7。
表2蛇床子素与白藜芦醇复配对齐整小核菌的室内生物活性测定
| 药剂名称及配比 | EC50(mg/L) | ATI | TTI | CTC |
| 蛇床子素 | 24.8923 | 100.0000 | -- | -- |
| 白藜芦醇 | 139.9665 | 17.7845 | -- | -- |
| 蛇床子素9：白藜芦醇1 | 24.5208 | 101.5150 | 91.7784 | 110.6088 |
| 蛇床子素8：白藜芦醇2 | 36.5088 | 68.1816 | 83.5569 | 81.5991 |
| 蛇床子素7：白藜芦醇3 | 39.1165 | 63.6363 | 75.3353 | 84.4707 |
| 蛇床子素6：白藜芦醇4 | 41.0724 | 60.6059 | 67.1138 | 90.3032 |
| 蛇床子素5：白藜芦醇5 | 49.7847 | 49.9999 | 58.8922 | 84.9007 |
| 蛇床子素4：白藜芦醇6 | 60.8479 | 40.9091 | 50.6707 | 80.7352 |
| 蛇床子素3：白藜芦醇7 | 46.9398 | 53.0303 | 42.4491 | 124.9266 |
| 蛇床子素2：白藜芦醇8 | 65.7158 | 37.8787 | 34.2276 | 110.6672 |
| 蛇床子素1：白藜芦醇9 | 80.3031 | 30.9979 | 26.0060 | 119.1952 |
由表2可知，蛇床子素与白藜芦醇复配时，当质量比为3：7时，复配组合对齐整小核菌的共毒系数大于120，表现出增效作用。
表3大蒜素与白藜芦醇复配对齐整小核菌的室内生物活性测定
由表3可知，大蒜素与白藜芦醇复配时，在1-9：9-1质量比范围内，所有供试的复配组合对齐整小核菌的共毒系数均大于120，表现出增效作用；尤其是当质量比为5：5时，复配组合对对齐整小核菌的共毒系数达到了2001.2197，增效作用最为显著。
表4蛇床子素与白藜芦醇复配对禾谷镰刀菌的室内生物活性测定
| 药剂名称及配比 | EC50(mg/L) | ATI | TTI | CTC |
| 蛇床子素 | 15.1544 | 100.0000 | -- | -- |
| 白藜芦醇 | 29.1159 | 52.0485 | -- | -- |
| 蛇床子素9：白藜芦醇1 | 12.8512 | 117.9221 | 95.2049 | 123.8614 |
| 蛇床子素8：白藜芦醇2 | 14.4173 | 105.1126 | 90.4097 | 116.2625 |
| 蛇床子素7：白藜芦醇3 | 12.2241 | 123.9715 | 85.6146 | 144.8019 |
| 蛇床子素6：白藜芦醇4 | 18.5829 | 81.5502 | 80.8194 | 100.9043 |
| 蛇床子素5：白藜芦醇5 | 9.0853 | 166.8013 | 76.0243 | 219.4054 |
| 蛇床子素4：白藜芦醇6 | 11.2162 | 135.1117 | 71.2291 | 189.6861 |
| 蛇床子素3：白藜芦醇7 | 28.7302 | 52.7473 | 66.4340 | 79.3981 |
| 蛇床子素2：白藜芦醇8 | 23.6259 | 64.1432 | 61.6388 | 104.0629 |
| 蛇床子素1：白藜芦醇9 | 21.3738 | 70.9018 | 56.8437 | 124.7311 |
由表4可知，蛇床子素与白藜芦醇复配时，当质量比为3：7时，复配组合对禾谷镰刀菌的共毒系数小于80，表现出拮抗作用；当质量比为8：2、6：4或2：8时，复配组合对禾谷镰刀菌的共毒系数在80-120之间，表现出相加作用；其余各复配组合对禾谷镰刀菌的共毒系数均大于120，表现出增效作用。
表5大蒜素与白藜芦醇复配对禾谷镰刀菌的室内生物活性测定
由表5可知，大蒜素与白藜芦醇复配时，当质量比为9-1：1-9时，复配组合对禾谷镰刀菌的共毒系数大于120，表现出增效作用。
表6蛇床子素与白藜芦醇复配对层出镰刀菌的室内生物活性测定
| 药剂名称及配比 | EC50(mg/L) | ATI | TTI | CTC |
| 蛇床子素 | 25.4976 | 100.0000 | -- | -- |
| 白藜芦醇 | 105.6220 | 24.1404 | -- | -- |
| 蛇床子素9：白藜芦醇1 | 25.0580 | 101.7543 | 92.4140 | 110.1070 |
| 蛇床子素8：白藜芦醇2 | 26.9142 | 94.7366 | 84.8281 | 111.6807 |
| 蛇床子素7：白藜芦醇3 | 30.9227 | 82.4559 | 77.2421 | 106.7499 |
| 蛇床子素6：白藜芦醇4 | 28.4974 | 89.4734 | 69.6562 | 128.4501 |
| 蛇床子素5：白藜芦醇5 | 29.0673 | 87.7192 | 62.0702 | 141.3225 |
| 蛇床子素4：白藜芦醇6 | 27.4974 | 92.7273 | 54.4843 | 170.1910 |
| 蛇床子素3：白藜芦醇7 | 38.2465 | 66.6665 | 46.8983 | 142.1512 |
| 蛇床子素2：白藜芦醇8 | 35.4479 | 71.9298 | 39.3123 | 182.9700 |
| 蛇床子素1：白藜芦醇9 | 33.7992 | 75.4385 | 31.7264 | 237.7784 |
由表6可知，蛇床子素与白藜芦醇复配时，当质量比为6-1：4-9质量比范围内，复配组合对层出镰刀菌的共毒系数均大于120，表现出增效作用；其余复配组合对层出镰刀菌的共毒系数在80-120之间，表现出增相加作用。
表7大蒜素与白藜芦醇复配对层出镰刀菌的室内生物活性测定
由表7可知，大蒜素与白藜芦醇复配时，当质量比为8：2或7：3或6：4时，复配组合对层出镰刀菌的共毒系数在80-120之间，表现出相加作用；其余个复配组合对层出镰刀菌的共毒系数均大于120，表现出增效作用。
综上所述，本发明将蛇床子素或大蒜素与白藜芦醇以一定质量比进行复配，对多种植物真菌性病害病原菌表现出协同增效作用，可以提高对植物病害的防治效果，为小麦茎基腐病、小麦赤霉病、花生根腐病、花生白绢病及玉米赤霉病的有效防控提供了数据支撑，且对小麦、花生、玉米等作物的优质高产具有重要的意义。
前述对本发明的具体示例性实施方案的描述是为了说明和例证的目的。这些描述并非想将本发明限定为所公开的精确形式，并且很显然，根据上述教导，可以进行很多改变和变化。对示例性实施例进行选择和描述的目的在于解释本发明的特定原理及其实际应用，从而使得本领域的技术人员能够实现并利用本发明的各种不同的示例性实施方案以及各种不同的选择和改变。本发明的范围意在由权利要求书及其等同形式所限定。
Claims (5)
1.一种含白藜芦醇的农药组合物，其特征在于，其有效成分由蛇床子素或大蒜素与白藜芦醇复配而成。
2.根据权利要求1所述的含白藜芦醇的农药组合物，其特征在于，所述蛇床子素与白藜芦醇的质量比为1-9：9-1。
3.根据权利要求1所述的含白藜芦醇的农药组合物，其特征在于，所述大蒜素与白藜芦醇的质量比为1-9：9-1。
4.根据权利要求1-3任一项所述的含白藜芦醇的农药组合物在防治农作物真菌性病害中的用途。
5.根据权利要求4所述的用途，其特征在于，所述农作物真菌性病害包括小麦茎基腐病、小麦赤霉病、花生根腐病、花生白绢病和玉米赤霉病。
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