CN1170788C - Method for raising phosphobacteria yield of biological fertilizer - Google Patents
Method for raising phosphobacteria yield of biological fertilizer Download PDFInfo
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- CN1170788C CN1170788C CNB001214551A CN00121455A CN1170788C CN 1170788 C CN1170788 C CN 1170788C CN B001214551 A CNB001214551 A CN B001214551A CN 00121455 A CN00121455 A CN 00121455A CN 1170788 C CN1170788 C CN 1170788C
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- phosphobacteria
- yield
- biological fertilizer
- raising
- aminated compounds
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The present invention relates to a method for raising the phosphobacteria yield of biological fertilizer. A liquid culture medium for phosphobacteria fermentation comprises a carbon source, a nitrogen source and inorganic salt, and the liquid culture medium is also added with an oxygenation increasing agent. The method for raising the phosphobacteria yield of biological fertilizer can in time meet the large demand of phosphobacteria for oxygen in a logarithmic growth phase and enhance the utilization rate of a nutrient source, and the method for raising the phosphobacteria yield of biological fertilizer obtains the maximum harvest quantity of the product from a consumptive institution medium material and enhances the speed and the number of the production and the propagation of the phosphobacteria. Compared with the traditional fermentation method, the method for raising the phosphobacteria yield of biological fertilizer can also raise 1 to 5 times of phosphobacteria yield, lower production cost and enhance fertilizer efficiency.
Description
Technical field
The present invention relates to a kind of method that improves phosphobacteria yield of biological fertilizer.
Background technology
In bio-feritlizer phosphorus bacteria fermenting process, the normal liquid culture of using must comprise carbon source, nitrogenous source, inorganic salts and the oxygen of q.s.The carbon, the nitrogen that promptly contain capacity, an amount of phosphorus, the inorganic salts of some sulfur-bearings, magnesium, calcium, potassium, sodium reaches elements such as micro-iron, copper, zinc, manganese.During the fermentation, the principal element that influences micropopulation bulk-growth and breeding has: solvend concentration etc. in the pH value of the selection of substratum, oxygen concentration, substratum, culture temperature, the substratum.After technical parameters such as substratum and pH value, temperature are preferably determined, the concentration of dissolved oxygen in the material (Dissolved oxygen) then with the growing of phosphorus bacteria. the quality and the quantity of pathways metabolism and product are closely related.Because oxidation of microorganism and biosynthesizing reaction are that the oxygen that is dissolved in the liquid that can only utilize that carries out in vivo is dissolved oxygen.Therefore in the aerobic fermentation process, adopt ventilation and stirring method to make usually and keep a certain amount of dissolved oxygen to satisfy oxygen required in the fermentation process in the nutrient solution.
Oxygen is the gas that is insoluble in water, and along with the increase of solute concentration in the rising of temperature and the water, the solubleness of oxygen in water also will descend.Strengthen air flow, improve stirring velocity, can increase the dissolved oxygen content in the nutrient solution to a certain extent, help the dissolving and the transfer process of oxygen.But still have the following disadvantages: 1, excessive ventilation and raising stirring velocity are removed a large amount of power of consumption, increase outside the cost, and high rotating speed can influence the form of some biological bacterium to a certain extent.The shearing action that stirs easily causes cell injury, self-dissolving ahead of time.Along with ventilation increases, air velocity is excessive, can make the paddle wheel overload, can not dispersion air, and ventilation efficiency no longer increases.2, the relative incubation time of oxygen requirement is a variable in the thalline fermenting process.The fermentation initial stage (being lag period) is not though material phenomenal growth in the thalline because of its total quantity is few, and has big the variation, so oxygen requirement is little.Along with the passing of incubation time, the thalline breeding enters logarithmic phase.Cell quantity is with geometric growth at this moment, and thalline is in the high-speed rapid growth animated period, and oxygen requirement constantly increases, until reaching highest level.Along with the increasing rapidly of phosphorus bacteria quantity, material viscosity strengthens gradually in addition, and the solubleness of oxygen also reduces gradually, and the oxygen supply aggravation of contradictions causes anoxic condition to take place, and influence synthesizing of product.The dissolved oxygen imbalance between supply and demand of this stage only is difficult to solve fully with the method that changes air flow and stirring velocity, thereby influences the yield of bio-feritlizer phosphorus bacteria.
Summary of the invention
The purpose of this invention is to provide a kind of method that improves phosphobacteria yield of biological fertilizer, to the heavy demand of oxygen, improve the speed and the quantity of thalli growth breeding when in time additional thalline is in logarithmic phase, reduce production costs, increase fertilizer efficiency.
The present invention improves the technical scheme of the method for phosphobacteria yield of biological fertilizer: at the liquid nutrient medium that is used for the phosphorus bacteria fermentation, except that containing carbon source, nitrogenous source, inorganic salts, it is characterized in that: also be added with oxygenation agent acyl peroxide aminated compounds (RCONH in the substratum
2O
2), the consumption of acyl peroxide aminated compounds is the 0.01-0.10% of substratum weight, described oxygenation agent acyl peroxide aminated compounds is to add in the fermentor tank after fermentation culture 1-8 hour.
The present invention improves the technical scheme two of the method for phosphobacteria yield of biological fertilizer: at the liquid nutrient medium that is used for the phosphorus bacteria fermentation, except that containing carbon source, nitrogenous source, inorganic salts, it is characterized in that: also be added with oxygenation agent acyl peroxide aminated compounds (RCONH in the substratum
2O
2), the consumption of acyl peroxide aminated compounds is the 0.01-0.10% of substratum weight, described oxygenation agent acyl peroxide aminated compounds is to add in the fermentor tank before the inoculation phosphorus bacteria.
The present invention improves the selected oxygenation agent of phosphobacteria yield of biological fertilizer method and possesses following feature: 1, water-soluble, by the pH value that does not change former system behind the proportional quantity adding nutrient solution; 2, oxygen release speed is fast, and the oxygenation effect is strong; 3, the growth and breeding to phosphorus bacteria does not have antagonism.
Advantage of the present invention: when in time replenishing thalline and being in logarithmic phase to the heavy demand of oxygen, improve the utilization ratio of nutrition source, from the unit matrix that is consumed, obtain the product of maximum harvest yield, improve the speed and the quantity of thalli growth breeding, improve phosphorus bacteria yield 1-5 doubly, reduce production costs, increase fertilizer efficiency.
Embodiment
Below in conjunction with example use of the present invention and effect are described in detail:
Respectively the available phosphorus bacterial count in following four embodiment products is detected with Plating (meeting the agricultural industry microbial fertilizer standard NY227-94 of the People's Republic of China (PRC)):
Fermentation culture conditions: culture temperature 28-37 ℃, aeration condition 1.0:0.8-1.5, fermentation period 20-35 hour, the phosphate-solubilizing bacteria of selecting for use is Bacillus megat erium, substratum: Semen Maydis powder 1.0-10.0 gram, bean powder 0.5-10.0 gram, starch 0.1-8.0 gram, potassium primary phosphate 0.1-0.5 gram, sodium-chlor 0.1-0.5 gram, lime carbonate 0.3-3.0 gram, sal epsom 0.2-1.0 gram, add water to 1000 milliliters, product phosphorus bacteria bacterium number is 0.8-1.0 * 10
9Individual/milliliter.
Embodiment one: add oxygenation agent peroxidation acid amides 0.1-1.0 gram before the inoculation phosphorus bacteria.Product phosphorus bacteria bacterium number is 1.8-2.4 * 10
9Individual/milliliter, phosphorus bacteria is counted yield and is improved 125-160%.
Embodiment two: add oxygenation agent peroxidation acid amides 0.1-1.0 gram after fermentation culture 1-8 hour, product bacterium bacterium number is 2.5-4.1 * 10
9Individual/milliliter, phosphorus bacteria is counted yield and is improved 210-360%.
Claims (2)
1, a kind of method that improves phosphobacteria yield of biological fertilizer, at the liquid nutrient medium that is used for the phosphorus bacteria fermentation, except that containing carbon source, nitrogenous source, inorganic salts, it is characterized in that: also be added with oxygenation agent acyl peroxide aminated compounds in the substratum, the consumption of acyl peroxide aminated compounds is the 0.01-0.10% of substratum weight, and described oxygenation agent acyl peroxide aminated compounds is to add in the fermentor tank after fermentation culture 1-8 hour.
2, a kind of method that improves phosphobacteria yield of biological fertilizer, at the liquid nutrient medium that is used for the phosphorus bacteria fermentation, except that containing carbon source, nitrogenous source, inorganic salts, it is characterized in that: also be added with oxygenation agent acyl peroxide aminated compounds in the substratum, the consumption of acyl peroxide aminated compounds is the 0.01-0.10% of substratum weight, and described oxygenation agent acyl peroxide aminated compounds is to add in the fermentor tank before the inoculation phosphorus bacteria.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001214551A CN1170788C (en) | 2000-07-24 | 2000-07-24 | Method for raising phosphobacteria yield of biological fertilizer |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB001214551A CN1170788C (en) | 2000-07-24 | 2000-07-24 | Method for raising phosphobacteria yield of biological fertilizer |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1319575A CN1319575A (en) | 2001-10-31 |
| CN1170788C true CN1170788C (en) | 2004-10-13 |
Family
ID=4588808
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB001214551A Expired - Fee Related CN1170788C (en) | 2000-07-24 | 2000-07-24 | Method for raising phosphobacteria yield of biological fertilizer |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1170788C (en) |
-
2000
- 2000-07-24 CN CNB001214551A patent/CN1170788C/en not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CN1319575A (en) | 2001-10-31 |
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