CN117070375A - Composite microbial agent and application thereof in ecological restoration of soil - Google Patents
Composite microbial agent and application thereof in ecological restoration of soil Download PDFInfo
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- 239000002689 soil Substances 0.000 title claims abstract description 61
- 230000000813 microbial effect Effects 0.000 title claims abstract description 26
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 22
- 239000002131 composite material Substances 0.000 title claims description 26
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 19
- 241000187747 Streptomyces Species 0.000 claims abstract description 17
- 239000003513 alkali Substances 0.000 claims abstract description 17
- 101710152191 Low molecular weight antigen MTB12 Proteins 0.000 claims abstract description 16
- 241000179039 Paenibacillus Species 0.000 claims abstract description 15
- 238000000034 method Methods 0.000 claims abstract description 7
- 241000894006 Bacteria Species 0.000 claims abstract description 5
- 150000001875 compounds Chemical class 0.000 claims abstract description 4
- 239000002068 microbial inoculum Substances 0.000 claims description 25
- 241000228143 Penicillium Species 0.000 claims description 17
- 239000001963 growth medium Substances 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims description 11
- 239000007787 solid Substances 0.000 claims description 11
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- 239000002054 inoculum Substances 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 claims description 3
- 238000002156 mixing Methods 0.000 claims description 3
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- 230000008635 plant growth Effects 0.000 abstract description 6
- 150000003839 salts Chemical class 0.000 abstract description 6
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 abstract description 3
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 abstract description 3
- 229910052698 phosphorus Inorganic materials 0.000 abstract description 3
- 239000011574 phosphorus Substances 0.000 abstract description 3
- 239000011591 potassium Substances 0.000 abstract description 3
- 229910052700 potassium Inorganic materials 0.000 abstract description 3
- 229910052710 silicon Inorganic materials 0.000 abstract description 3
- 239000010703 silicon Substances 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000035558 fertility Effects 0.000 abstract description 2
- 235000002233 Penicillium roqueforti Nutrition 0.000 abstract 1
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 abstract 1
- 210000004215 spore Anatomy 0.000 description 41
- 238000011282 treatment Methods 0.000 description 16
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- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 5
- 238000009629 microbiological culture Methods 0.000 description 5
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 4
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- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 239000012153 distilled water Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 229920001817 Agar Polymers 0.000 description 3
- 241000193830 Bacillus <bacterium> Species 0.000 description 3
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 3
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- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 2
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- 241001047076 Paenibacillus xylanilyticus Species 0.000 description 2
- 241001507683 Penicillium aurantiogriseum Species 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 241000187180 Streptomyces sp. Species 0.000 description 2
- 230000001580 bacterial effect Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 230000015784 hyperosmotic salinity response Effects 0.000 description 2
- 239000011159 matrix material Substances 0.000 description 2
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- 239000000203 mixture Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
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- 229910052757 nitrogen Inorganic materials 0.000 description 2
- 230000035699 permeability Effects 0.000 description 2
- 238000005067 remediation Methods 0.000 description 2
- 230000008439 repair process Effects 0.000 description 2
- 238000003892 spreading Methods 0.000 description 2
- 230000007480 spreading Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 241000186063 Arthrobacter Species 0.000 description 1
- 241000589941 Azospirillum Species 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 1
- 229910021536 Zeolite Inorganic materials 0.000 description 1
- YUWBVKYVJWNVLE-UHFFFAOYSA-N [N].[P] Chemical compound [N].[P] YUWBVKYVJWNVLE-UHFFFAOYSA-N 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 210000004666 bacterial spore Anatomy 0.000 description 1
- 239000002585 base Substances 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 238000004820 blood count Methods 0.000 description 1
- PASHVRUKOFIRIK-UHFFFAOYSA-L calcium sulfate dihydrate Chemical compound O.O.[Ca+2].[O-]S([O-])(=O)=O PASHVRUKOFIRIK-UHFFFAOYSA-L 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000003245 coal Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- HNPSIPDUKPIQMN-UHFFFAOYSA-N dioxosilane;oxo(oxoalumanyloxy)alumane Chemical compound O=[Si]=O.O=[Al]O[Al]=O HNPSIPDUKPIQMN-UHFFFAOYSA-N 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 230000004720 fertilization Effects 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 230000035784 germination Effects 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000011866 long-term treatment Methods 0.000 description 1
- 235000009973 maize Nutrition 0.000 description 1
- 230000000877 morphologic effect Effects 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 238000004382 potting Methods 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000002688 soil aggregate Substances 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000010457 zeolite Substances 0.000 description 1
- 229910000859 α-Fe Inorganic materials 0.000 description 1
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
- B09C1/105—Reclamation of contaminated soil microbiologically, biologically or by using enzymes using fungi or plants
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- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/685—Aspergillus niger
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- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/80—Penicillium
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Abstract
The invention relates to a compound bacteria microbial agent which comprises aspergillus niger CA-5, blue mold CFP-2, paenibacillus CBP-2 and streptomyces CS13-6, and has good saline-alkali tolerance and strong colonization capability, effectively releases elements such as silicon, phosphorus, potassium and the like in soil, reduces the pH and salt content of the soil, increases the soil fertility and obviously promotes the plant growth. The method has practical economic benefit and wide application prospect in ecological restoration of soil, especially in restoration of saline-alkali soil.
Description
Technical Field
The invention relates to the field of environmental microorganisms, in particular to a composite microbial agent and application thereof in ecological restoration of soil.
Background
Soil salinization can cause soil hardening, and air permeability, water permeability are poor, the salinity is high, pH is high, causes farmland nutrient loss serious, and nitrogen phosphorus nutrient utilization efficiency is lower, is unfavorable for crops growth, easily causes crops to reduce yield even die. Saline-alkali soil treatment is a worldwide problem, and over 100 countries worldwide suffer from salinized soil. The area of the Chinese saline soil is about 1 hundred million hm 2 Wherein, the area of various saline soil which can be treated, repaired and utilized is 0.36 hundred million hm 2 Can be developed and utilized to be more than 0.067 hundred million hm of saline-alkali soil with agricultural development prospect 2 . China is one of countries with large distribution area, wide area and serious pollution of salinized soil, and is urgent to promote ecological treatment of the salinized soil and research on theory and technology of salinization prevention and control.
The main restoration methods for the long-term treatment of the saline-alkali soil by people are as follows: (1) The physical repair method mainly adopts the measures of drainage, flushing, soil loosening, fertilization, sand spreading, alkali pressing and the like; (2) The chemical restoration method is mainly used for restoring the saline-alkali soil through chemical reaction between soil colloidal particles and exogenous additives, such as adding substances such as zeolite, peat, phosphogypsum, weathered coal and the like; (3) The bioremediation method mainly comprises the steps of planting salt-tolerant plants to repair the saline-alkali soil, and meanwhile, many reports on repairing the saline-alkali soil by utilizing microorganisms are also provided.
At present, the discovered saline-alkali tolerant growth promoting strains comprise pseudomonas, azospirillum, arthrobacter, bacillus, enterobacter and the like, but the soil system is equivalent to a complex microecological system, the repairing mechanism of each strain is not completely the same, a large amount of novel high-efficiency functional microorganisms are required to be excavated according to actual needs, and the soil repairing efficiency is effectively improved by combining different microorganism repairing mechanisms. The microbial remediation microbial inoculum with low cost and no environmental pollution is developed, the soil quality is improved, and the microbial remediation microbial inoculum has important significance for developing green ecological agriculture.
Disclosure of Invention
The invention aims to provide a composite microbial inoculum which has good high salt tolerance and strong colonization capacity and remarkably promotes plant growth, and simultaneously provides application of the composite microbial inoculum in soil ecological restoration.
The invention relates to the following technical scheme:
a composite microbial inoculum comprises Aspergillus niger CA-5, penicillium poland CFP-2, paenibacillus CBP-2 and Streptomyces CS13-6.
Further, the ratio of the spore numbers of the Aspergillus niger CA-5, the Penicillium polum CFP-2, the Paenibacillus CBP-2 and the Streptomyces CS13-6 is 30-55:20-40:10-20:15-25.
Further, in the composite microbial inoculum, the total spore number is not less than 1 multiplied by 10 9 CFU/g。
Wherein the collection number of the Aspergillus niger (Aspergillus niger) CA-5 is CGMCC No.40271, and the Aspergillus niger is collected in the common microorganism center of China Committee for culture Collection of microorganisms, and the address is the institute of microorganisms of national academy of sciences of China, national academy of sciences of China, no. 3, with a collection date of 2022, 8 months and 12 days.
Wherein, the preservation number of the Penicillium polentarum (Penicillium polonicum) CFP-2 is CGMCC No.40494, and the Penicillium polentarum is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms), the address is Beijing, and the preservation date is 2023, 2 and 8.
Wherein, the collection number of the paenibacillus (Paenibacillus xylanilyticus) CBP-2 is CGMCC No.26727, and the paenibacillus is preserved in the China general microbiological culture Collection center with the address of Beijing in China and the preservation date of 2023, 3 months and 3 days.
Wherein the preservation number of the Streptomyces (Streptomyces sp.) CS13-6 is CGMCC No.25523, and the Streptomyces is preserved in China general microbiological culture Collection center with the address of Beijing, and the preservation date is 2022, 8 months and 12 days.
A preparation method of the composite microbial inoculum comprises the steps of respectively growing aspergillus niger CA-5 and Penicillium polenta CFP-2 in a PDA solid flat plate culture medium until spore production, growing paenibacillus CBP-2 in a PB liquid culture medium until spore production, and culturing streptomyces CS3-16 in a Gao's No. 1 culture medium until spore production. Cleaning spores with sterile water, counting the content of each spore in spore suspension, and mixing according to a proportion to obtain the compound bacterial spore liquid.
An application of the composite microbial inoculum in soil ecological restoration.
In application, the soil is saline-alkali soil and can also be used for alkaline tailing area soil.
The invention has the beneficial effects that: the composite microbial agent consists of various probiotics, has good acid-base and high salt tolerance, has strong colonization capability, and enables all strains to cooperate and synergistically increase a great amount of beneficial bacteria in soil, can restore diversity of soil microbial communities, has the effect of releasing silicon, can also dissolve phosphorus, potassium, fix nitrogen, produce ferrite and IAA, not only improves soil nutrients, reduces soil pH and salt content and increases soil aggregate structure and organic matter content, but also obviously promotes plant growth, and has practical economic benefit and wide application prospect in saline-alkali soil restoration.
Detailed Description
The invention is further illustrated below with reference to examples. The scope of the invention is not limited to the examples, and any modifications within the scope of the claims are within the scope of the invention.
Example 1 preparation of Complex microbial inoculant
(1) Preparation of Aspergillus niger CA-5 spore liquid
The collection number of the Aspergillus niger (Aspergillus niger) CA-5 is CGMCC No.40271, and the Aspergillus niger is collected in the China general microbiological culture center of the China Committee for culture Collection of microorganisms, and the address is the institute of microorganisms, national academy of sciences of China, no. 3, north Chen West Lu 1, korea, beijing, china, and the collection date is 2022, 8 months and 12 days.
Picking part of Aspergillus niger CA-5 spores by an inoculating loop, uniformly inoculating the spores on a PDA solid flat-plate culture medium, culturing for 3-5 days at 35 ℃, scraping spores on the surface of the PDA solid culture medium, placing the spores in 20mL of sterile water containing glass beads, shaking by a shaking table at 200rpm at 35 ℃ for 20-30 min to prepare spore suspension, counting by a hemocytometer, wherein the concentration of the Aspergillus niger CA-5 spore liquid is more than or equal to 1 multiplied by 10 9 CFU·mL -1 。
PDA medium (g/L): 200g of potato, 20g of glucose, 1L of distilled water and natural pH. The solid culture medium needs to be added with 15-20 g of agar.
(2) Preparation of Penicillium Polaromanii CFP-2 spore liquid
The preservation number of the Penicillium polenta (Penicillium polonicum) CFP-2 is CGMCC No.40494, and the Penicillium polenta is preserved in the China general microbiological culture Collection center (China Committee for culture Collection of microorganisms), the address is Beijing, and the preservation date is 2023, 2 and 8.
Picking part of the blue penicillium CFP-2 spores by an inoculating loop, uniformly inoculating the blue penicillium CFP-2 spores on a PDA solid flat plate culture medium, culturing for 3-5 days at 25 ℃, scraping the spores on the surface of the PDA solid culture medium, placing the spores in 20mL of sterile water containing glass beads, shaking for 20-30 min at 25 ℃ with a shaking table at 200rpm to prepare spore suspension, counting by a blood cell counting plate, wherein the concentration of the blue penicillium CFP-2 spore liquid is not less than 1 multiplied by 10 9 CFU·mL -1 。
PDA medium (g/L): 200g of potato, 20g of glucose and 1L of distilled water, and 15-20 g of agar is added into a solid culture medium, so that the pH is natural.
(3) Paenibacillus CBP-2 spore liquid preparation
The collection number of the paenibacillus (Paenibacillus xylanilyticus) CBP-2 is CGMCC No.26727, and the paenibacillus is collected in the common microorganism center of China Committee for culture Collection of microorganisms, the address is Beijing, and the collection date is 2023, 3 months and 3 days.
Picking with inoculating loopInoculating part of bacillus CBP-2 on PB liquid culture medium, culturing at 28deg.C for 2-3 d, observing with smear microscope, stopping culturing when more than 80% is spore, counting on plate to make the concentration of bacillus CBP-2 spore liquid not less than 1×10 9 CFU·mL -1 。
PB liquid medium: 10g of peptone, 5g of beef extract, 5g of NaCl, 1L of distilled water and pH of 7.0-7.2.
(4) Preparation of Streptomyces CS13-6 spore liquid
The preservation number of the Streptomyces (Streptomyces sp.) CS13-6 is CGMCC No.25523, and the Streptomyces is preserved in China general microbiological culture Collection center with the address of Beijing, and the preservation date is 2022, 8 months and 12 days.
Picking part of Streptomyces CS13-6 spores by an inoculating loop, uniformly inoculating on a solid plate culture medium of Gao's No. 1, culturing for 5-8 d at 28 ℃, scraping spores on the surface of the solid culture medium, placing the spores in 20mL of sterile water containing glass beads, shaking by a shaking table at 200rpm at 25 ℃ for 20-30 min to prepare spore suspension, counting by a hemocytometer, wherein the concentration of the Streptomyces CS13-6 spore liquid is not less than 1X 10 9 CFU·mL -1 。
Culture medium No. 1, gao: KNO (KNO) 3 1g, soluble starch 20g, naCl 0.5g, K 2 HPO 4 ·3H 2 O 0.5g、MgSO 4 ·7H 2 O 0.5g、FeSO 4 ·7H 2 O0.01 g, distilled water 1L, pH 7.4-7.6, and agar 15-20 g.
(4) Mixing of composite bacterial agents
According to Table 1, spore suspensions of Aspergillus niger CA-5, penicillium polentam CFP-2, paenibacillus CBP-2 and Streptomyces CS13-6 were mixed to obtain a composite microbial agent having total spore count of not less than 1×10 9 CFU/mL。
Table 1 compounding ratio of the composite microbial agent
Comparative example
Aspergillus niger CA-5 spore prepared in example 1 was used as a control 1.
The Penicillium poland CFP-2 spores prepared in example 1 were used as a comparative bacteria 2.
Paenibacillus CBP-2 spores prepared in example 1 were used as a reference bacterial agent 3.
Streptomyces CS13-6 spores prepared in example 1 were used as a comparative agent 4.
In each of the above comparative bacteria, the total spore amount is not less than 1×10 9 CFU/g。
Experimental example 1 Effect of composite microbial Agents on lettuce growth
Healthy lettuce seeds with uniform size and plump seeds are randomly selected, and the surface of the seeds is disinfected by using 70% ethanol and 3% NaClO water solution. Seedling raising of lettuce: uniformly spreading the purchased commodity seedling culture matrix into a seedling culture tray, uniformly spraying seeds on the seedling culture tray, covering the seedling culture tray with about 0.5cm matrix, and watering without exposing the seeds. And (5) watering the seedling raising tray thoroughly with water after seeding, and placing the seedling raising tray in a greenhouse for cultivation.
Lettuce field planting: when lettuce grows to the center of three leaves, selecting lettuce seedlings with the same size from a seedling raising tray, transplanting the lettuce seedlings into a flowerpot with the diameter of 15cm, and taking potting soil from saline-alkali soil in Hebei Cangzhou, wherein the salt content of the soil is 0.41%, and the pH value is 8.2. The experiment sets 9 treatments, the blank control CK is not added with the microbial inoculum, and the other 8 treatments mix 1-4 of the compound microbial inoculum and four single microbial inoculum (comparative microbial inoculum 1-4) into soil, wherein the spore concentration in each gram of soil is 2 multiplied by 10 6 CFU, 10 pots are treated each time, the method is repeated for three times, the daytime room temperature is set to 28 ℃, the illumination is carried out for 12 hours, the nighttime room temperature is set to 24 ℃, the darkness time is set to 12 hours, and the single plant yield, plant height, leaf number, leaf length and leaf width of lettuce are measured in 25-30 days.
The influence of different microbial agents on the growth of potted lettuce is shown in table 2, the treatment of adding the microbial agents has a remarkable promotion effect on the growth of lettuce, and various growth indexes of lettuce are remarkably improved, wherein the effect of the composite microbial agents is remarkably better than that of the single microbial agent treatment, and the single microbial agent treatment is remarkably better than that of a control group. The application of the composite microbial inoculum to the lettuce planting in the saline-alkali soil has more remarkable promotion effect on lettuce growth.
TABLE 2 Effect of microbial agents on lettuce plant growth
Note that: the result is an average (standard error) of repeated measurements; different lower case letters represent significant differences between treatments (P < 0.05).
Experimental example 2 Effect of composite microbial Agents on corn growth and soil Properties
The soil is obtained from saline-alkali soil in Cangzhou, hebei, and has salt content of 0.45% and pH of 8.7. The experiment sets 9 treatments, the blank control CK is not added with any microbial inoculum, and the other 8 treatments mix 1-4 composite microbial inoculum and four single microbial inoculum (comparative microbial inoculum 1-4) into soil, wherein the spore concentration in each gram of soil is 2 multiplied by 10 6 CFU, 10 pots per treatment, repeated three times, placed in a greenhouse at 25 ℃, after germination of the seeds, three seedlings with the best growth in each pot are reserved, 6 repeats are set in each group, and water is periodically replenished. In addition, the added microbial inoculum group was irrigated with 2-5 mL spore suspension in each pot every 15d, and the morphological characteristics of the plants were regularly observed and recorded in contrast to the same amount of sterile water. After 40d, the overground index of soil and plants (corn) and the growth condition of root systems are measured.
TABLE 3 Effect of different microbial agents on maize plant growth
Note that: the result is an average (standard error) of repeated measurements; different lower case letters represent significant differences between treatments (P < 0.05).
TABLE 4 Effect of different microbial agents on soil Properties
Note that: the result is an average (standard error) of repeated measurements; different lower case letters represent significant differences between treatments (P < 0.05).
The results show (see tables 3 and 4): the corn treated by the microbial inoculum is obviously superior to the control treatment in plant growth vigor and root system, and the treatment by the composite microbial inoculum is obviously superior to the single microbial inoculum treatment. The contents of effective silicon, quick-acting potassium, effective phosphorus and total nitrogen in the soil are obviously improved, the single microbial inoculum is improved by more than 20% compared with the control, and the composite microbial inoculum is improved by more than 35% compared with the control. At the same time, the salt content and pH in the soil decrease significantly. Further illustrates that the application of the functional microbial agent in the soil can not only remarkably improve the plant yield and reduce the pH and salt content of the soil, but also remarkably improve the soil fertility and the soil quality, and can be used for the production and application of the bio-organic fertilizer or the improvement of the saline-alkali soil.
The present invention is described in detail with reference to the above embodiments. It should be noted that the above embodiments are merely illustrative of the invention. Numerous alternatives and modifications of the present invention will be devised by those skilled in the art without departing from the spirit and nature of the invention, which should be construed as being within the scope of the present invention.
Claims (8)
1. A composite microbial inoculum is characterized by comprising aspergillus niger CA-5, penicillium poland CFP-2, paenibacillus CBP-2 and streptomyces CS13-6.
2. The composite microbial inoculant according to claim 1, wherein the ratio of spores of aspergillus niger CA-5, penicillium polum CFP-2, paenibacillus CBP-2 and streptomyces CS13-6 is 30-55:20-40:10-20:15-25.
3. The composite microbial inoculant according to claim 1, wherein the total spore count is not less than 1X 10 10 CFU/g。
4. A composite microbial agent according to any one of claims 1 to 3, wherein the collection number of aspergillus niger CA-5 is CGMCC No.40271, the collection number of penicillium variotium CFP-2 is CGMCC No.40494, the collection number of paenibacillus CBP-2 is CGMCC No.26727, and the collection number of streptomyces CS13-6 is CGMCC No.25523.
5. A method for preparing the composite microbial inoculum according to claim 1-4, which is characterized in that aspergillus niger CA-5 and Penicillium polenta CFP-2 are grown in a PDA solid plate culture medium until spores are produced; paenibacillus CBP-2 grows to produce spores in PB liquid culture medium; streptomyces CS13-6 grows to produce spores in a solid culture medium of Gaoshan No. 1; washing spores from a flat plate by using sterile water, counting the spore content in spore suspension, and mixing according to a proportion to obtain the compound bacteria spore liquid.
6. The application of the composite microbial inoculant according to claim 1-4 in soil ecological restoration.
7. The use according to claim 6, wherein the soil is saline-alkali soil.
8. The use according to claim 6, wherein the soil is alkaline tailings area soil.
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| CN115505536A (en) * | 2022-10-12 | 2022-12-23 | 河北省科学院生物研究所 | Aspergillus niger CA-5 and application thereof |
| CN116676201A (en) * | 2023-08-01 | 2023-09-01 | 河北省科学院生物研究所 | Penicillium poland CFP-2 and application thereof |
| CN117050913A (en) * | 2023-08-24 | 2023-11-14 | 河北省科学院生物研究所 | Paenibacillus CBP-2 and application thereof |
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| CN115505536A (en) * | 2022-10-12 | 2022-12-23 | 河北省科学院生物研究所 | Aspergillus niger CA-5 and application thereof |
| CN116676201A (en) * | 2023-08-01 | 2023-09-01 | 河北省科学院生物研究所 | Penicillium poland CFP-2 and application thereof |
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