CN117070349A - Culture dish for improving activity of stem cells based on red light irradiation principle - Google Patents

Culture dish for improving activity of stem cells based on red light irradiation principle Download PDF

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CN117070349A
CN117070349A CN202311088815.5A CN202311088815A CN117070349A CN 117070349 A CN117070349 A CN 117070349A CN 202311088815 A CN202311088815 A CN 202311088815A CN 117070349 A CN117070349 A CN 117070349A
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fixedly connected
culture dish
subassembly
culture
culture solution
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CN117070349B (en
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许清霖
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Hangzhou Chengyou Biotechnology Co ltd
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Hangzhou Chengyou Biotechnology Co ltd
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Abstract

The application relates to a culture dish for improving the activity of stem cells based on a red light irradiation principle, which comprises a culture dish component, wherein a box body component is arranged on the outer side of the culture dish component, an illumination component is arranged on the lower side of the culture dish component, the illumination component is in sliding connection with the inner wall of the box body component, an adjusting component is arranged at the top of the illumination component and is fixedly connected with the illumination component, and a temperature control component is fixedly connected with the bottom of the box body component.

Description

Culture dish for improving activity of stem cells based on red light irradiation principle
Technical Field
The application relates to a culture dish for improving the activity of stem cells based on a red light irradiation principle, in particular to a culture dish for improving the activity of stem cells based on a red light irradiation principle, which is applied to the technical field of cell culture.
Background
Red light, particularly near infrared light (NIR, wavelength range of approximately 650-1000 nm), has unique biostimulation effects on cells and tissues. Research has shown that red light can promote cell viability, enhance cell repair, enhance ATP (adenosine triphosphate) production, reduce oxidative stress, and thereby provide necessary energy for various biological processes, and stem cells are a class of cells that have the ability to self-renew and differentiate into multiple cell types because of this unique ability, and stem cells are seen as having great potential in tissue repair and regenerative medicine, and in recent years, research has found that red light irradiation can effectively increase the viability of stem cells, promote their proliferation, migration and differentiation, possibly due to red light stimulating the photosensitive receptors of cells, such as cytochrome c oxidase, thereby activating a range of biological responses of cells.
The specification of Chinese patent No. 112210497 discloses a long-time incubation system of living cells combined with an LED array and an optogenetic living cell imaging method, wherein the system is used in a living cell workstation and provides a portable and stable culture environment for long-time living cell imaging experiments. Besides a gas supply system, a temperature control system and a closed culture cabin chamber for providing stable culture conditions for cells, the system is additionally provided with an LED array for assisting optogenetic experiments. The LED array is formed by alternately arranging LED lamp beads with emission wavelengths of 488nm (blue light), 561nm (green light) and 640nm (red light), and the emission wavelength of the LED array can be changed in real time according to the requirement. The light emitting mode of the LED array is changed by the controller, and the current working state of the LED array is displayed in real time. The light emitted by the LED array finally irradiates horizontally to a culture dish positioned in the center of the closed culture cabin, and the imaging light path of the living cell workstation is not affected.
The design can realize a long-time incubation system of living cells for optogenetic cell research, and also needs to ensure that an imaging light path of a living cell workstation is not influenced, but has certain defects, particularly in the stem cell culture process, the activity of the stem cells cannot be improved by utilizing red light irradiation.
Content of the application
The application aims to solve the technical problem of providing a culture dish which is convenient to improve the activity of stem cells by using red light irradiation.
In order to solve the problems, the application provides a culture dish for improving the activity of stem cells based on a red light irradiation principle, which comprises a culture dish component, wherein a box component is arranged on the outer side of the culture dish component, an illumination component is arranged on the lower side of the culture dish component, the illumination component is in sliding connection with the inner wall of the box component, an adjusting component is arranged at the top of the illumination component and is fixedly connected with the illumination component, a temperature control component is fixedly connected with the bottom of the box component, a culture solution control component is fixedly connected with the top of the culture dish component, a control system is fixedly connected with the top of the culture dish component and is electrically connected with the illumination component, the temperature control component and the culture solution control component through wires, and the adjusting component and the temperature control component are fixedly connected through pipelines.
In the culture dish of stem cell liveness is improved to above-mentioned based on red light irradiation principle, carry out red light irradiation to stem cell through illumination subassembly, help improving the liveness of stem cell, the fixed connection design of adjusting part and illumination subassembly makes illumination intensity and angle adjustable, satisfy different cultivation demands, the temperature control subassembly of box subassembly bottom can ensure the temperature stability of culture environment, provide best growth environment for stem cell, the design of culture solution control assembly and control system for the interpolation, change and the control of culture solution are more convenient and accurate.
As a further improvement of the application, the culture dish assembly comprises a culture dish body, a filter screen is clamped in the culture dish body, the bottom of the filter screen is fixedly connected with the top of the culture dish body, a sink groove is arranged between the culture dish body and the filter screen, a culture dish bracket is clamped at the outer end of the culture dish body, and the outer end of the culture dish bracket is fixedly connected with the box body assembly.
As a still further improvement of the application, the box body assembly comprises an upper box body fixedly connected with the outer end of the culture dish support, a top cover is fixedly connected to the top of the upper box body, a partition plate is fixedly connected to the bottom of the upper box body, a lower box body is fixedly connected to the bottom of the partition plate, an illumination assembly and an adjusting assembly are slidably connected to the inner wall of the lower box body, a temperature control assembly is fixedly connected to the bottom of the lower box body, a culture solution control assembly and a control system are fixedly connected to the top of the top cover, the partition plate is made of transparent materials, the upper box body, the top cover and the lower box body are made of non-transparent materials, and light absorption materials are coated on the inner walls of the upper box body, the top cover and the lower box body.
As a further improvement of the application, the illumination component comprises a lamp panel which is in sliding connection with the inner wall of the lower box body, a plurality of lamp beads are fixedly connected to the top of the lamp panel, the lamp beads are uniformly distributed around the center of the lamp beads, a cooling cavity is formed in the lamp beads, an adjusting component is fixedly connected to the bottom of the cooling cavity, a light guide plate is fixedly connected to the top of the lamp beads, the light guide plate is in sliding connection with the inner wall of the lower box body, the lamp beads are red in wavelength-nm, and the lamp beads are electrically connected with the control system through wires.
As a further improvement of the application, the adjusting component comprises a cylinder sleeve fixedly connected with the bottom of the lamp panel, a piston is slidably connected with the inner wall of the cylinder sleeve, a reset spring is fixedly connected with the top of the light guide plate, and the top of the reset spring is fixedly connected with the bottom of the partition plate.
As a further improvement supplement of the application, the temperature control component comprises a water bath pipe fixedly connected with the outer end of the upper box body and symmetrically arranged, the water bath pipe is communicated with the inner part of the upper box body, the water bath pipe is positioned at the top of the partition plate, one end of the water bath pipe, which is far away from the upper box body, is fixedly connected with a temperature controller, the temperature controller is positioned at the bottom of the box body component, the temperature controller is fixedly connected with a heat regeneration cooling pipe which is symmetrically arranged, one end of the heat regeneration cooling pipe, which is far away from the temperature controller, is fixedly connected with a lamp panel, the water bath pipe and the heat regeneration cooling pipe are respectively fixedly connected with an electromagnetic valve, the water bath pipe, the temperature controller, the heat regeneration cooling pipe and the electromagnetic valve are mutually communicated, sterile purified water is filled in the communicating part, the electromagnetic valve and the temperature controller are electrically connected with a control system through wires, and the water bath pipe and the temperature controller are flexible hoses.
As a further improved supplement of the application, the culture solution control assembly comprises a culture solution tank fixedly connected with the top of the top cover, a baffle is fixedly connected in the culture solution tank, the baffle divides the interior of the culture solution tank into a new culture solution tank and an old culture solution tank, a peristaltic pump is fixedly connected to the bottom of the new culture solution tank, a feeding pipe is fixedly connected to one end of the peristaltic pump far away from the new culture solution tank, a discharging pipe is fixedly connected to the top of the old culture solution tank, one end of the discharging pipe far away from the old culture solution tank is fixedly connected with the culture dish body, the culture solution is filled in the new culture solution tank, the peristaltic pump is electrically connected with the control system through a wire, a through hole is formed in the top of the baffle, and a filter layer is fixedly connected in the through hole.
As a further improvement of the present application, the control system includes a main control board, an integrated microprocessor for processing inputs of various sensors and controlling various actuators, a temperature sensor for detecting real-time temperature of the culture dish assembly and transmitting data to the main control board, an illumination sensor for detecting intensity and wavelength of red light emitted from the illumination assembly, a liquid level sensor for detecting amount of culture liquid in the culture liquid control assembly, a user interface for displaying real-time data and setting parameters, and a manual control device, a communication module for remote monitoring and control by Wi-Fi or Bluetooth, power management, ensuring that all the assemblies are properly powered, and a standby power supply for preventing power failure.
In summary, the scheme has the following beneficial effects:
1. cell liveness improvement: the red light irradiation can effectively improve the activity of stem cells and create favorable conditions for the growth and differentiation of the cells.
2. The illumination is flexibly adjusted: the light intensity and the angle of illumination can be accurately adjusted by combining the adjusting component and the illumination component, the requirements of stem cells in different stages and types are met, the partition plate is made of transparent materials, the non-transparent design of the upper box body, the top cover and the lower box body is combined, the accurate illumination of light is ensured, a plurality of lamp beads are evenly distributed around the center, the illumination uniformity is ensured, the cooling cavity is designed to be beneficial to the cooling of the lamp beads, the service life of the lamp beads is prolonged, the sliding connection design of the lamp panel and the light guide plate and the lower box body provides the adjustment flexibility of illumination distance, and the illumination intensity can be adaptively adjusted by the illumination component through the design of the piston and the reset spring.
3. Stable temperature environment: the temperature control component at the bottom of the box body component ensures that the temperature of the culture environment is always stable, and provides an optimal growth environment for stem cells.
4. Accurate control of the culture solution: by combining the culture solution control assembly and the control system, the addition, replacement and circulating flow of the culture solution can be realized more conveniently and accurately, stem cells in the culture solution can not flow out due to the design of the filter screen, the culture solution is allowed to flow freely, and the culture solution after filtration can be effectively converged due to the design of the confluence tank, so that the subsequent treatment and circulation are convenient.
5. And (3) integrated management: the control system is electrically connected with each component, so that integrated management of the whole cultivation process is realized, the convenience of operation is improved, and a user can conveniently control the wavelength and illumination intensity of the lamp beads through the electrical connection with the control system.
6. And (3) heat recycling: the heat generated by the lamp panel can be recovered through the design of the backheating cooling tube and the lamp panel.
Drawings
FIG. 1 is an overall block diagram of the present application;
FIG. 2 is a front view of the present application;
FIG. 3 is a cross-sectional view A-A of the present application;
FIG. 4 is a B-B cross-sectional view of the present application;
FIG. 5 is a C-C cross-sectional view of the present application;
FIG. 6 is an enlarged view of the application at D;
FIG. 7 is an enlarged view of the application at E;
FIG. 8 is a partial block diagram of the culture dish body of the present application;
FIG. 9 is a partial block diagram of a lamp panel, lamp beads and cylinder liners of the present application;
fig. 10 is an external configuration view of the present application.
The reference numerals in the figures illustrate:
1. a culture dish assembly; 101. a culture dish body; 102. a filter screen; 103. a sink groove; 104. a culture dish support; 2. a housing assembly; 201. an upper case; 202. a top cover; 203. a partition plate; 204. a lower box body; 3. an illumination assembly; 301. a lamp panel; 302. a lamp bead; 303. a cooling chamber; 304. a light guide plate; 4. an adjustment assembly; 401. cylinder sleeve; 402. a piston; 403. a return spring; 5. a temperature control assembly; 501. a water bath tube; 502. a temperature controller; 503. a heat returning cooling pipe; 504. an electromagnetic valve; 6. a culture fluid control assembly; 601. a culture solution tank; 602. a baffle; 603. a new culture liquid tank; 604. old culture fluid tanks; 605. a peristaltic pump; 606. a feed pipe; 607. a discharge pipe; 7. and a control system.
Detailed Description
Four embodiments of the present application will be described in detail with reference to the accompanying drawings.
First embodiment:
fig. 1, 2 and 10 show.
The utility model provides a culture dish based on red light shines principle and improves stem cell liveness, including culture dish subassembly 1, culture dish subassembly 1 outside is equipped with box subassembly 2, culture dish subassembly 1 downside is equipped with illumination subassembly 3, illumination subassembly 3 and box subassembly 2 inner wall sliding connection, illumination subassembly 3 top is equipped with adjusting part 4, adjusting part 4 and illumination subassembly 3 fixed connection, box subassembly 2 bottom fixedly connected with control by temperature change subassembly 5, culture dish subassembly 1 top fixedly connected with culture solution control assembly 6, culture dish subassembly 1 top fixedly connected with control system 7, control system 7 passes through wire and illumination subassembly 3, control by temperature change subassembly 5 and control by temperature change subassembly 6 electric connection, adjusting part 4 and control by temperature change subassembly 5 pass through pipeline fixed connection.
In the culture dish of stem cell liveness is improved to above-mentioned based on red light irradiation principle, carry out the red light through illumination subassembly 3 to stem cell and shine, help improving the liveness of stem cell, the fixed connection design of adjusting part 4 and illumination subassembly 3 makes illumination intensity and angle adjustable, satisfy different cultivation demands, the temperature control subassembly 5 of box subassembly 2 bottom can ensure the temperature stability of culture environment, provide the best growth environment for stem cell, the design of culture solution control subassembly 6 and control system 7, make the interpolation of culture solution, change and control more convenient and accurate.
Preparation prior to use, stem cells are obtained by, but not limited to, obtaining stem cells by methods such as, for example, extraction of embryonic stem cells, typically from day-old embryos, extraction of adult stem cells from a variety of tissues, such as bone marrow, fat, skin, etc., induction of pluripotent stem cells, reprogramming by introducing specific transcription factors into the adult cells, dissociation of cells, and dissociation of cells from tissues using enzymes such as pancreatin or collagenase.
When the device is used, the device is firstly subjected to comprehensive cleaning and disinfection, the box body assembly 2 is opened to take out the culture dish assembly 1, dissociated cells are planted in the culture dish assembly 1, specific culture mediums are used for culturing according to requirements, the culture mediums contain nutrient substances and growth factors required by cell growth, the control system 7 controls the regulating assembly 4 and the temperature control assembly 5 to regulate and control sterile purified water at the outer end of the culture dish assembly 1 to a proper temperature, then the culture dish assembly 1 with planted cells is placed in the box body assembly 2, the culture dish assembly 1 is maintained at a constant temperature through the control system 7 controls the regulating assembly 4 and the temperature control assembly 5 in the cell culturing process, the control system 7 controls the illumination assembly 3 to irradiate stem cells in the culture dish assembly 1 when required, the control system 7 controls the wavelength and the illumination intensity of the illumination assembly 3 to improve the activity of the stem cells, meanwhile, the control system 7 controls the culture solution control assembly 6 to inject new culture solution into the culture dish assembly 1 according to requirements, old culture solution is discharged, the culture solution is circulated, the culture solution is well circulated, and the cell is taken out after the culture is completed, and the device is closed.
The red light irradiation enhances the activity, the activity of stem cells can be effectively improved through the red light irradiation of the illumination component 3, favorable conditions are created for the growth and differentiation of the cells, the flexible illumination adjustment is realized, the combination design of the adjustment component 4 and the illumination component 3 enables the illumination intensity and angle to be accurately adjusted, the requirements of different stages and types of stem cells are met, the temperature of the culture environment is always stable through the temperature control component 5 at the bottom of the box component 2, the optimal growth environment is provided for the stem cells, the culture solution is accurately controlled, the combination of the culture solution control component 6 and the control system 7 ensures that the cells are always in an optimal culture state, the integrated control system 7 ensures that the addition, the replacement and the circulation flow of the culture solution are more convenient and accurate, the integrated control system 7 is electrically connected with each component, the integrated management of the whole culture process is realized, and the control from the illumination and the temperature to the culture solution can be completed through one system, so that the convenience of operation is improved.
Second embodiment:
fig. 3, 4, 7 and 8 show.
Culture dish subassembly 1 includes culture dish body 101, joint has filter screen 102 in the culture dish body 101, filter screen 102 bottom and culture dish body 101 top fixed connection, be equipped with the sink 103 between culture dish body 101 and the filter screen 102, culture dish body 101 outer end joint has culture dish support 104, culture dish support 104 outer end and box subassembly 2 fixed connection, box subassembly 2 includes the last box 201 with culture dish support 104 outer end fixed connection, go up box 201 top fixedly connected with top cap 202, go up box 201 bottom fixedly connected with baffle 203, baffle 203 bottom fixedly connected with lower box 204, lower box 204 inner wall sliding connection has illumination subassembly 3 and adjusting part 4, lower box 204 bottom fixedly connected with temperature control subassembly 5, top cap 202 top fixedly connected with culture solution control subassembly 6 and control system 7, baffle 203 is transparent material and makes, go up box 201, top cap 202 and lower box 204 are non-transparent material and go up box 201, top cap 202 and lower box 204 inner wall all scribble the light absorbing material.
During the use, plant the stem cell that will wait to cultivate on culture dish body 101, afterwards, on inserting culture dish body 101 to culture dish support 104, utilize culture dish support 104 to fix culture dish body 101, after the culture solution flows into on culture dish body 101, under the action of gravity, the culture solution flows around culture dish body 101 gradually, after the culture solution flows through filter screen 102, filter screen 102 filters the culture solution, prevent the stem cell of culture dish body 101 from flowing out, the culture solution after filtering flows into confluence groove 103, and assemble in confluence groove 103, go up box 201, top cap 202 and baffle 203 form a space and are used for fixed culture dish support 104, simultaneously with control by temperature change subassembly 5 are connected, utilize control by temperature change subassembly 2 inside to carry out temperature control, baffle 203 is made for the transparent material and is convenient for make baffle 203 bottom the light to shine the baffle 203 upside through baffle 203, go up box 201, top cap 202 and lower box 204 are all made for non-transparent material, and go up box 201, top cap 202 and lower box 204 inner wall all scribble the material, be convenient for stop outside to shine and go up the box 201 and produce the reflection-type light absorption effect on the inside the reflection of reflection-absorbing material more than a plurality of times, can be avoided or the light reflection-absorbing effect is produced inside the reflection-absorbing material after the light is avoided.
Cell protection and filtration, the design of filter screen 102 has ensured that stem cells in the culture solution can not flow out, simultaneously allow the culture solution free flow, a stable growing environment has been provided for the cell, effectual liquid management, the existence of confluence tank 103 makes the culture solution after the filtration can assemble effectively, the convenience is subsequent handling and circulation, the stable structure, culture dish support 104 and box subassembly 2's fixed connection has ensured the stability of culture dish, the unexpected removal or the slope that lead to because of the external factor has been avoided, temperature control subassembly 5's design makes the temperature of culture environment be in the best state all the time, the advantage is provided for the growth of stem cells, illumination management, baffle 203 is made for transparent material, make light can direct irradiation to the culture dish, and the light is only shone from predetermined direction to the non-transparent design of upper box 201, top cap 202 and lower box 204 and the light-absorbing material coating of inner wall, the interference of external light has been avoided, the controllability of illumination has been strengthened, comprehensive control system 7, culture solution control subassembly 6 and control system 7 on the top cap 202 can be for whole because of unexpected removal or slope that the external factor leads to the illumination, the integrated control can be carried out to the light-absorbing system, the additional illumination can be carried out to the optimal light-absorbing material for the growth of the cell, the top cap is more accurate, the light-absorbing system is avoided, the top cap is more than stable, the light-absorbing system is carried out, the light is more than stable, and the light is difficult to be flowed down, and has been avoided, the light is more stable is difficult to the light is easy.
Third embodiment:
fig. 3, 5 and 9 show.
The illumination assembly 3 comprises a lamp panel 301 which is in sliding connection with the inner wall of the lower box 204, a plurality of lamp beads 302 are fixedly connected to the top of the lamp panel 301, the lamp beads 302 are uniformly distributed around the center of the lamp panel, a cooling cavity 303 is formed in the lamp beads 302, an adjusting assembly 4 is fixedly connected to the bottom of the cooling cavity 303, a light guide plate 304 is fixedly connected to the top of the lamp beads 302, the light guide plate 304 is in sliding connection with the inner wall of the lower box 204, the lamp beads 302 are red in wavelength 630-670nm, the lamp beads 302 are electrically connected with the control system 7 through wires, the adjusting assembly 4 comprises a cylinder sleeve 401 fixedly connected with the bottom of the lamp panel 301, a piston 402 is slidably connected to the inner wall of the cylinder sleeve 401, a reset spring 403 is fixedly connected to the top of the light guide plate 304, and the top of the reset spring 403 is fixedly connected with the bottom of the partition 203.
When the light guide plate is used, the wavelength and illumination intensity of the lamp beads 302 are controlled through the control system 7 according to the needs, the cooling cavity 303 is used for cooling the lamp plate 301 and the lamp beads 302, the lamp beads 302 are kept at a proper temperature, the light guide plate 304 can regulate light emitted by the lamp beads 302, the light guide plate is distributed more uniformly, the lamp plate 301 and the light guide plate 304 are in sliding connection with the inner wall of the lower box 204, so that the lamp plate 301 and the light guide plate 304 can slide up and down along the lower box 204, illumination intensity and illumination effect are further regulated through changing illumination distance, when the pressure in the lamp plate 301 is increased, the cylinder sleeve 401 moves upwards along the axis direction of the piston 402 under the action of the pressure, the cylinder sleeve 401 further drives the lamp plate 301 to move upwards, the lamp plate 301 drives the light guide plate 304 to move upwards, the light guide plate 304 presses the reset spring 403 in the upward moving process, the reset spring 403 is compressed due to the fact that the top of the reset spring 403 is fixedly connected with the bottom of the partition 203, cells in the process that the light source is close to culture can provide illumination with higher intensity, and when the pressure of the lamp plate 301 is reduced, and the elasticity of the reset spring 403 under the action of the reset spring can drive the light guide plate 304 to move downwards to the light guide plate 304 to move towards the cell culture plate 304.
Even illumination, a plurality of lamp pearls 302 evenly distribute around the center, the homogeneity of illumination has been ensured, a uniform illumination environment has been provided for the cell, effective cooling, the cooling chamber 303's design helps the cooling of lamp pearl 302, avoid the overheated because of long-time work leads to, the life of lamp pearl 302 has been prolonged and illumination's stability has been ensured, the light is adjusted, the existence of light guide plate 304 can adjust the light that lamp pearl 302 sent, make the light more concentrated and even, the effect of illumination has been improved, flexible adjustment, the sliding connection design of lamp plate 301 and light guide plate 304 and lower box 204 makes the illumination distance can adjust as required, provide bigger flexibility of operation for the user, can adjust illumination intensity and effect according to actual demand, self-adaptation illumination intensity adjusts, through piston 402 and reset spring 403's design, illumination subassembly 3 can adjust according to internal pressure self-adaptation, make illumination intensity self-adaptation, both convenient and high-efficient, specific wavelength, the red light that the lamp pearl 302 sent, the wavelength is at 630-670nm, this kind of specific wavelength is to the growth that can promote the division of cell and can control the illumination intensity, the integrated control with the illumination system is convenient for the illumination intensity, 7, can control the illumination system is easy and convenient for the control of illumination system, the illumination intensity and control of the cell.
Fourth embodiment:
fig. 3 and 6 show.
The temperature control assembly 5 comprises a water bath pipe 501 fixedly connected with the outer end of the upper box 201 and symmetrically arranged, the water bath pipe 501 is communicated with the inside of the upper box 201, the water bath pipe 501 is positioned at the top of the partition 203, one end of the water bath pipe 501 far away from the upper box 201 is fixedly connected with a temperature controller 502, the temperature controller 502 is positioned at the bottom of the box assembly 2, the temperature controller 502 is fixedly connected with a symmetrically arranged heat return cooling pipe 503, one end of the heat return cooling pipe 503 far away from the temperature controller 502 is fixedly connected with a lamp plate 301, electromagnetic valves 504 are fixedly connected to the water bath pipe 501 and the heat return cooling pipe 503, the water bath pipe 501, the temperature controller 502, the heat return cooling pipe 503 and the electromagnetic valves 504 are mutually communicated, sterile purified water is filled in the communicating part, the electromagnetic valves 504 and the temperature controller 502 are electrically connected with the control system 7 through wires, the water bath pipe 501 and the temperature controller 502 are flexible hoses, the culture solution control assembly 6 comprises a culture solution tank 601 fixedly connected with the top cover 202, a baffle 602 is fixedly connected in the culture solution tank 601, the baffle 602 divides the interior of the culture solution tank 601 into a new culture solution tank 603 and an old culture solution tank 604, a peristaltic pump 605 is fixedly connected to the bottom of the new culture solution tank 603, one end of the peristaltic pump 605, far away from the new culture solution tank 603, is fixedly connected with a feed pipe 606, the top of the old culture solution tank 604 is fixedly connected with a discharge pipe 607, one end, far away from the old culture solution tank 604, of the discharge pipe 607 is fixedly connected with the culture dish body 101, culture solution is filled in the new culture solution tank 603, the peristaltic pump 605 is electrically connected with the control system 7 through wires, a through hole is formed in the top of the baffle 602, and a filter layer is fixedly connected with in the through hole.
When the temperature in the upper box 201 needs to be regulated, water is heated or cooled by the control system 7 to control 505, and meanwhile, the control system 7 can conduct heat in the lamp plate 301 into the upper box 201 as required, so that the heat of the lamp plate 301 is recycled, when the heat of the lamp plate 301 needs to be recycled and the temperature in the upper box 201 is lower than the temperature in the lamp plate 301, the control system 7 controls the electromagnetic valve 504 on the water bath pipe 501 and the backheating cooling pipe 503 to enable water in the lamp plate 301 to flow into the temperature controller 502, the water in the temperature controller 502 flows into the water bath pipe 501 to be mixed with the water bath pipe 501 flowing into the temperature controller 502, then flows back into the water bath pipe 501 under the pushing of the temperature controller 502, so that the upper box 201 is heated, when new culture solution needs to be added into the culture dish body 101, the peristaltic pump 605 is controlled to operate by the control system 7, a baffle 602 is fixedly connected with the inside the baffle 602 through the baffle plate 602, and the filter layer 607 is opened, and the fresh culture solution is circulated in the culture dish body and the culture tank 603 by the suction of the air pressure of the culture tank 101, and the old culture tank is sucked into the culture tank 101 after the filter layer 602.
The control system 7 comprises a main control board, an integrated microprocessor for processing the inputs of the various sensors and controlling the various actuators, a temperature sensor for detecting the real-time temperature of the culture dish assembly 1 and transmitting data to the main control board, an illumination sensor for detecting the intensity and wavelength of the red light emitted by the illumination assembly 3, a liquid level sensor for detecting the amount of culture liquid in the culture liquid control assembly 6, a user interface for displaying real-time data and setting parameters, and a manual control device, a communication module for remote monitoring and control by Wi-Fi or bluetooth, power management, ensuring that all the assemblies are properly powered and have a standby power to prevent power failure.
The accurate temperature control can accurately regulate the temperature in the upper box 201 through the design of the water bath pipe 501 and the temperature controller 502, ensure the stability of the cell culture environment, recycle the heat generated by the lamp panel 301 through the design of the backheating cooling pipe 503 and the lamp panel 301, improve the utilization efficiency of energy sources, automatically update and circulate the culture solution through the design of the peristaltic pump 605, the feed pipe 606 and the discharge pipe 607, ensure the freshness of the cell culture solution, realize the backflow of the culture solution by air pressure through the design of the filter layer at the top of the baffle 602, improve the circulation efficiency of the culture solution and integrate the control system 7, the control system 7 includes a main control board, a microprocessor and various sensors, and can monitor and adjust various parameters in real time, ensure the stability and efficiency of cell culture, monitor and display data in real time, the user interface design enables the user to check various parameters in real time and perform manual adjustment, and thus, the operation convenience is improved, the remote monitoring and control is performed, the design of the communication module enables the user to perform remote monitoring and control through Wi-Fi or bluetooth, the operation flexibility is improved, the power management design ensures that all components are properly powered, and a standby power supply is also provided in case of power failure, the stable operation of the device is ensured, the flexible hose design of the water bath pipe 501 and the temperature controller 502 provides greater operation flexibility, the device is not easy to be blocked when moving, the illumination intensity and the wavelength are precisely controlled, the illumination sensor can detect the intensity and the wavelength of the red light emitted by the illumination component 3, so that the illumination condition of cell culture can be ensured to meet the requirements.
The present application is not limited to the above-described embodiments, which are adopted in connection with the actual demands, and various changes made by the person skilled in the art without departing from the spirit of the present application are still within the scope of the present application.

Claims (9)

1. The application relates to a culture dish for improving the activity of stem cells based on a red light irradiation principle, which comprises a culture dish group and is characterized in that: including culture dish subassembly (1), culture dish subassembly (1) outside is equipped with box subassembly (2), culture dish subassembly (1) downside is equipped with illumination subassembly (3), illumination subassembly (3) and box subassembly (2) inner wall sliding connection, illumination subassembly (3) top is equipped with adjusting part (4), adjusting part (4) and illumination subassembly (3) fixed connection, box subassembly (2) bottom fixedly connected with control by temperature change subassembly (5), culture dish subassembly (1) top fixedly connected with culture solution control assembly (6), culture dish subassembly (1) top fixedly connected with control system (7), control system (7) pass through wire and illumination subassembly (3), control by temperature change subassembly (5) and culture solution control assembly (6) electric connection, adjusting part (4) and control by temperature change subassembly (5) pass through pipeline fixed connection.
2. The culture dish for improving the viability of stem cells based on the red light irradiation principle according to claim 1, wherein: culture dish subassembly (1) are including culture dish body (101), joint has filter screen (102) in culture dish body (101), filter screen (102) bottom and culture dish body (101) top fixed connection, be equipped with sink (103) between culture dish body (101) and filter screen (102), culture dish body (101) outer end joint has culture dish support (104), culture dish support (104) outer end and box subassembly (2) fixed connection.
3. The culture dish for improving the viability of stem cells based on the red light irradiation principle according to claim 2, wherein: the box body assembly (2) comprises an upper box body (201) fixedly connected with the outer end of the culture dish support (104), a top cover (202) is fixedly connected to the top of the upper box body (201), a partition plate (203) is fixedly connected to the bottom of the upper box body (201), a lower box body (204) is fixedly connected to the bottom of the partition plate (203), an illumination assembly (3) and an adjusting assembly (4) are slidably connected to the inner wall of the lower box body (204), a temperature control assembly (5) is fixedly connected to the bottom of the lower box body (204), a culture solution control assembly (6) and a control system (7) are fixedly connected to the top of the top cover (202), the partition plate (203) is made of transparent materials, and the inner walls of the upper box body (201), the top cover (202) and the lower box body (204) are all coated with light absorbing materials.
4. A culture dish for improving the viability of stem cells based on the red light irradiation principle according to claim 3, wherein: the illumination assembly (3) comprises a lamp panel (301) which is slidably connected with the inner wall of the lower box body (204), a plurality of lamp beads (302) are fixedly connected to the top of the lamp panel (301), the lamp beads (302) are uniformly distributed around the center of the lamp beads, a cooling cavity (303) is formed in the lamp beads (302), an adjusting assembly (4) is fixedly connected to the bottom of the cooling cavity (303), a light guide plate (304) is fixedly connected to the top of the lamp beads (302), the light guide plate (304) is slidably connected with the inner wall of the lower box body (204), the lamp beads (302) are red, the wavelengths of 630-670nm, and the lamp beads (302) are electrically connected with the control system (7) through wires.
5. The dish for improving the viability of stem cells based on the red light irradiation principle according to claim 4, wherein: the adjusting component (4) comprises a cylinder sleeve (401) fixedly connected with the bottom of the lamp panel (301), a piston (402) is slidably connected to the inner wall of the cylinder sleeve (401), a reset spring (403) is fixedly connected to the top of the light guide plate (304), and the top of the reset spring (403) is fixedly connected with the bottom of the partition plate (203).
6. A culture dish for improving the viability of stem cells based on the red light irradiation principle according to claim 3, wherein: the temperature control assembly (5) comprises water bath pipes (501) fixedly connected with the outer ends of the upper box bodies (201) and symmetrically arranged, the water bath pipes (501) are communicated with the inner parts of the upper box bodies (201), the water bath pipes (501) are located at the tops of the partition boards (203), and one ends, far away from the upper box bodies (201), of the water bath pipes (501) are fixedly connected with temperature controllers (502).
7. The culture dish for improving the viability of stem cells based on the red light irradiation principle according to claim 6, wherein: temperature controller (502) are located box subassembly (2) bottom, backheating cooling pipe (503) that fixedly connected with symmetry set up on temperature controller (502), backheating cooling pipe (503) are kept away from temperature controller (502) one end and lamp plate (301) fixed connection, all fixedly connected with solenoid valve (504) on water bath pipe (501) and backheating cooling pipe (503), water bath pipe (501), temperature controller (502), backheating cooling pipe (503) and solenoid valve (504) are inside to communicate each other, and the inside aseptic pure water that has all filled of intercommunication part, solenoid valve (504) and temperature controller (502) are all through wire and control system (7) electric connection, water bath pipe (501) and temperature controller (502) are flexible hose.
8. A culture dish for improving the viability of stem cells based on the red light irradiation principle according to claim 3, wherein: culture solution control assembly (6) include with top cap (202) top fixed connection's culture solution case (601), fixedly connected with baffle (602) in culture solution case (601), baffle (602) are with culture solution case (601) inside new culture solution groove (603) and old culture solution groove (604), new culture solution groove (603) bottom fixedly connected with peristaltic pump (605), new culture solution groove (603) one end fixedly connected with inlet pipe (606) are kept away from in peristaltic pump (605), old culture solution groove (604) top fixedly connected with discharging pipe (607), old culture solution groove (604) one end and culture dish body (101) fixed connection are kept away from in discharging pipe (607), new culture solution groove (603) intussuseption is filled with the culture solution, peristaltic pump (605) are through wire and control system (7) electric connection, the through-hole has been seted up at baffle (602) top, and through-hole internal fixedly connected with filter layer.
9. The culture dish for improving the viability of stem cells based on the red light irradiation principle according to claim 1, wherein: the control system (7) comprises a main control board, a temperature sensor, an illumination sensor, a liquid level sensor, a user interface, a communication module and a power management module.
CN202311088815.5A 2023-08-28 2023-08-28 Culture dish for improving activity of stem cells based on red light irradiation principle Active CN117070349B (en)

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Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012077980A2 (en) * 2010-12-07 2012-06-14 (주)루트로닉 Stem cell culturing apparatus
CN112210497A (en) * 2020-10-14 2021-01-12 度微检测技术(杭州)有限公司 Long-time living cell incubation system combined with LED array and optogenetic living cell imaging method
CN213416910U (en) * 2020-09-24 2021-06-11 河南省北科生物科技有限公司 Cell culture case that suitability is strong
CN218811764U (en) * 2022-12-20 2023-04-07 中国人民解放军总医院第六医学中心 Cell culture case

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2012077980A2 (en) * 2010-12-07 2012-06-14 (주)루트로닉 Stem cell culturing apparatus
CN213416910U (en) * 2020-09-24 2021-06-11 河南省北科生物科技有限公司 Cell culture case that suitability is strong
CN112210497A (en) * 2020-10-14 2021-01-12 度微检测技术(杭州)有限公司 Long-time living cell incubation system combined with LED array and optogenetic living cell imaging method
CN218811764U (en) * 2022-12-20 2023-04-07 中国人民解放军总医院第六医学中心 Cell culture case

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