CN106434283A - Diatom culture plate-type reactor - Google Patents
Diatom culture plate-type reactor Download PDFInfo
- Publication number
- CN106434283A CN106434283A CN201610984641.4A CN201610984641A CN106434283A CN 106434283 A CN106434283 A CN 106434283A CN 201610984641 A CN201610984641 A CN 201610984641A CN 106434283 A CN106434283 A CN 106434283A
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- reactor
- diatom
- box
- type reactor
- casing
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/18—Flow directing inserts
- C12M27/20—Baffles; Ribs; Ribbons; Auger vanes
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/06—Nozzles; Sprayers; Spargers; Diffusers
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
- C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M31/00—Means for providing, directing, scattering or concentrating light
- C12M31/10—Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/06—Means for regulation, monitoring, measurement or control, e.g. flow regulation of illumination
Abstract
The invention relates to a bioreactor for high-density culture of microalgae, in particular to a diatom culture plate-type reactor. According to the technical scheme, the diatom culture plate-type reactor is composed of a reactor box, a circulating reaction system, a lighting system and a control system, wherein the reactor box is of box structure, the circulating reaction system comprises a box external circulating portion and a box internal circulating portion, the control system is a programmable logic controller, and the lighting system is disposed at the reactor box and is composed of a plurality of groups of LED sources. The diatom culture plate-type reactor has the advantages that an interlayer guide plate is combined with LEDs and the like in the diatom culture plate-type reactor such that algal liquid uses more space of the reactor box; the diatom culture plate-type reactor is provided with a circulating pump communicated with the reactor box and connected to a liquid inlet, mobile cultivation of algae is achieved, uniformity of the algal liquid is ensured, and since the circulating pump is a diaphragm pump, less shear action is applied to algal cells.
Description
Technical field
The present invention relates to a kind of bioreactor of High Density Cultivation microalgae, more particularly to a kind of for diatom culture plate
Formula reactor.
Background technology
Diatom is the unicellular alga that a class has chromatoplast.Diatom species is various, whole world kind about more than 1.6 ten thousand.
The features such as individual little, growth and breeding is fast, metabolic activity is high, growth cycle is short, raw rich in polyphenol, unsaturated fatty acid, pigment etc.
Active substances.In diatom body, main photosynthetic pigments have chlorophyll a, Chlorofucsin, beta-carotene, fucoxanthin, silicon dinoflagellate Huang
Element etc..With going deep into for research, diatom is in the application of the field such as aquatic feed, food and medicine, bio-fuel, nano material and environmental protection
Advantage is increasingly highlighted.Especially in diatom unsaturated fatty acid and pigment achievement in research, disclose microalgae source unsaturation
Fatty acid and the high bioactivity of fucoxanthin, can provide for the production of healthy oils and fatss and antioxidant drug may.Therefore,
High Density Cultivation diatom technology and the research and development of exclusive device, are the key links of technical transform.
Under the suitable environment of Photosynthetic Conditions, diatom can quickly breed and obtain larger Biomass.Due to diatom
Without flagellum, it is impossible to the free swimming in water as other flagellum microalgae, therefore usually bottom is deposited on, its photosynthesis is affected,
Growth heterogeneity.Existing microalgae reactor has horizontal tube, vertical tube type, the soft form such as packed, but all can not be well
Frustule deposition in diatom incubation is solved the problems, such as, makes diatom training quality unstable.This patent designs a kind of board-like silicon
Algae culturing device, on the basis of traditional bioreactor, adds PLC control cabinet to the parameter in incubation, carries out standard
True control and adjustment；The tide of nature is simulated using circulating pump, using interlayer deflector, makes diatom during flowing
Be circulated culture, both avoided because diatom in a large number deposition and between the algae that causes cell interception, in turn ensure that diatom with
Culture matrix is fully contacted, so as to realize the high-density growth of diatom.
Content of the invention
For the problem of frustule deposition in existing incubation, the present invention simulates the tide of nature by circulating pump
Nighttide, makes diatom be circulated culture in flow regime, while the accurate control by PLC control cabinet to diatom condition of culture parameter
System, provides a kind of High Density Cultivation that cultivates plate-type reactor for diatom, realize to diatom.
For solving the above problems, the present invention is adopted the technical scheme that：The board-like cultivation reactor of diatom is by reactor casing
Constitute with circulating reaction system, illumination system and control system.Wherein described reactor casing is a box-structure；Cyclic system
System is made up of case outer circulation part and casing interior circulation part, collectively forms blood circulation；Control system selects FPGA
Controller（PLC）, PLC is connected with blood circulation, illumination system by some wires；Illumination system is arranged at reactor casing,
It is made up of some groups of LED light sources.
Described reactor casing, is case structure, and the side of upper surface is provided with air vent and filler pipe, and opposite side sets
Dog-house is equipped with, side is provided with air inlet gas supplementing opening.When culture fluid concentration is too high, or during cleaning, can be added water by filler pipe；
Incubation supplements air by air inlet gas supplementing opening into reactor.The bottom surface of reactor casing is provided with discharge nozzle and culture
Liquid extraction tube.
Case outer circulation part in described blood circulation is made up of suction tube, circulating pump and feed pipe, wherein suction tube
It is connected with culture fluid extraction tube and circulating pump, feed pipe is connected with circulating pump and reactor casing upper side.Start circulating pump
The culture fluid of reactor box bottom can be pumped into the top in reactor casing through feed pipe.
Described casing interior circulation part, is made up of 4-8 piece interlayer deflector；Described interlayer deflector, upper inferior
Away from, be dislocated arrangement, three side of interlayer deflector is connected with reactor box inside plate, and another side is stayed with reactor box side
Interval is had to form overfall.So it is easy to the culture fluid on upper interlayer deflector to flow into next interlayer deflector from overfall
On.
Described interlayer deflector, is arranged in 5-10 degree gradient, and one end of overfall is in low level.
Described illumination system, has 4-8 group, constitutes per group 2-3 bar LED, is arranged at going to the bottom for piece interlayer deflector
Face, is connected with PLC per bar LED, needs the number of light filling and light filling amount by PLC control.
Beneficial effects of the present invention are：1st, diatom is cultivated board-like reactor assembly and is provided with PLC control cabinet, can monitor at any time anti-
Parameter is answered, is regulated and controled, it is ensured that the quality of production of diatom.2nd, diatom culture plate-type reactor sandwich establishment deflector and LED etc.
Combine, space hold of the algae solution to reaction casing is improve, interlayer deflector is thin, and relative area is big, by flowed step by step
Mode is realized being fully contacted for algae solution and light source and not only can reduce the mutual shading of iuntercellular that diatom causes because of a large amount of depositions
Effect, can also effectively exclude the dissolved oxygen accumulation in photosynthesis, be that the high density high-quality culture of diatom provides strong profit
Growing environment.3rd, board-like diatom culture apparatuses are provided with circulating pump and reaction chamber body phase is logical, and access inlet, so as to realize frond
Flowing culture, it is ensured that algae solution homogeneity, and circulating pump uses membrane pump, reduces the shear action to frustule.
Description of the drawings
Fig. 1 is that diatom of the present invention cultivates board-like device front longitudinal sectional drawing.
Fig. 2 is that diatom of the present invention cultivates board-like device side longitudinal sectional drawing.
Specific embodiment
With reference to specification drawings and specific embodiments, the substantive distinguishing features of the present invention are described further.
In Fig. 1,1 is reactor casing；2 is interlayer deflector；3 is dog-house；4 is air inlet gas supplementing opening；5 is diatom culture
Liquid discharging opening；6 is culture fluid extraction tube；7 is suction tube；8 is circulating pump；9 is feed pipe；10 is filler pipe；11 is exhaustor.
In Fig. 2,2 is interlayer deflector；6 is culture fluid extraction tube；9 is feed pipe；11 is exhaustor；12 is LED
Light bar.
Embodiment 1
Using said structure, before starting diatom culture, first from dog-house（3）Diatom seed algae solution is put into, from filler pipe（10）
The appropriate culture fluid of middle injection.When the culture fluid of injection is mixed with seed algae solution and is deposited on reactor casing（1）Behind bottom, will
PLC is opened, and starts circulating pump（8）, set circulating pump（8）Working time, off time and algae solution flow velocity, lightening LED lamp
Band（12）, by circulating pump（8）By culture fluid seed algae solution mixed liquor by feed pipe（9）Pump in reactor.Pump into reactor
Interior mixed liquor flows through interlayer deflector（2）, in LED（12）Irradiation under, successively flow through interlayer deflector（2）, progressively
Complete the flowing culture of diatom.Incubation can pass through culture fluid extraction tube（6）Extract real-time culture fluid, tests growth of diatom algae
Density.After culture terminates, then cut out circulating pump controls by PLC（8）And LED（12）, open diatom culture fluid discharging opening
（5）, terminate culture.
Diatom species is various, and different types of growth of diatom algae condition difference, according to the growth characteristics of all kinds of algaes, using PLC
Can stable regulation LED（12）Intensity of illumination, periodicity of illumination, and control constant voltage pass through filler（10）, air inlet QI invigorating
Mouthful（4）Carry out water is sent, supply gas, all parameters can be adjusted according to culture situation, an excellent life to be created to diatom
Long environment, realizes the high-density growth of diatom.
Claims (6)
1. a kind of diatom cultivates plate-type reactor, it is characterised in that the board-like cultivation reactor of described diatom by reactor casing and
Circulating reaction system, illumination system and control system are constituted, and wherein described reactor casing is a box-structure；Blood circulation
It is made up of case outer circulation part and casing interior circulation part；Control system be programmable logic controller (PLC) and by some wires and
Blood circulation, illumination system are connected；Illumination system is arranged at reactor casing, is made up of some groups of LED light sources.
2. a kind of diatom according to claim 1 cultivates plate-type reactor, it is characterised in that described reactor casing, is
Case structure, the side of upper surface is provided with air vent and filler pipe, and opposite side is provided with dog-house, and side is provided with air inlet benefit
QI KOU；When culture fluid concentration is too high, or during cleaning, can be added water by filler pipe；Incubation is by air inlet gas supplementing opening to anti-
Answer supplemental oxygen in device；The bottom surface of reactor casing is provided with discharge nozzle and culture fluid extraction tube.
3. a kind of diatom according to claim 1 cultivates plate-type reactor, it is characterised in that in described blood circulation
Case outer circulation part is made up of suction tube, circulating pump and feed pipe, and wherein suction tube is connected with culture fluid extraction tube and circulating pump,
Feed pipe is connected with circulating pump and reactor casing upper side.
4. a kind of diatom according to claim 1 cultivates plate-type reactor, it is characterised in that described casing interior circulation portion
Point, it is made up of 4-8 piece interlayer deflector；Described interlayer deflector, up and down equidistantly, is dislocated arrangement, interlayer deflector
Three sides are connected with reactor box inside plate, another side and the spaced formation overfall of reactor box side.
5. a kind of diatom according to claim 1 cultivates plate-type reactor, it is characterised in that described interlayer deflector, is in
5-10 degree gradient is arranged, and one end of overfall is in low level.
6. a kind of diatom according to claim 1 cultivates plate-type reactor, it is characterised in that described illumination system, has 4-
8 groups, constitute per group 2-3 bar LED, the bottom surface of piece interlayer deflector is arranged at, is connected with PLC per bar LED.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610984641.4A CN106434283A (en) | 2016-11-09 | 2016-11-09 | Diatom culture plate-type reactor |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201610984641.4A CN106434283A (en) | 2016-11-09 | 2016-11-09 | Diatom culture plate-type reactor |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN106434283A true CN106434283A (en) | 2017-02-22 |
Family
ID=58208743
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201610984641.4A Pending CN106434283A (en) | 2016-11-09 | 2016-11-09 | Diatom culture plate-type reactor |
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| Country | Link |
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| CN (1) | CN106434283A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107603875A (en) * | 2017-10-30 | 2018-01-19 | 乐山晟嘉电气股份有限公司 | A kind of intelligent biological bacterium culture machine and cultural method |
| CN109355190A (en) * | 2018-10-31 | 2019-02-19 | 北海生巴达生物科技有限公司 | A kind of method that microalgae recycling increases dissolved oxygen with water |
| CN110257221A (en) * | 2019-06-24 | 2019-09-20 | 王晨 | A kind of microalgae bioreactor purifying air and the sunshading board based on the microalgae bioreactor |
| CN110616139A (en) * | 2019-10-15 | 2019-12-27 | 青岛融智汇海洋生物科技股份有限公司 | Process and system for industrially producing biological silicon |
| EP3585876A4 (en) * | 2017-02-23 | 2020-12-23 | Algaennovation Ltd | System and method for growing algae |
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| CN2257291Y (en) * | 1996-06-25 | 1997-07-02 | 华南理工大学 | Enclosed circulating thin layer spirulina cultivating apparatus |
| CN1201826A (en) * | 1997-06-05 | 1998-12-16 | 华南师范大学 | Spirulina light illuminating tower-tray optic bio-reactor culturing system and its controlling method |
| CN104560702A (en) * | 2015-01-21 | 2015-04-29 | 北京化工大学 | Intelligent dimming self-cleaning pipe type photo-bioreactor |
| CN206751785U (en) * | 2016-11-09 | 2017-12-15 | 福建师范大学 | A kind of diatom cultivates plate-type reactor |
-
2016
- 2016-11-09 CN CN201610984641.4A patent/CN106434283A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2257291Y (en) * | 1996-06-25 | 1997-07-02 | 华南理工大学 | Enclosed circulating thin layer spirulina cultivating apparatus |
| CN1201826A (en) * | 1997-06-05 | 1998-12-16 | 华南师范大学 | Spirulina light illuminating tower-tray optic bio-reactor culturing system and its controlling method |
| CN104560702A (en) * | 2015-01-21 | 2015-04-29 | 北京化工大学 | Intelligent dimming self-cleaning pipe type photo-bioreactor |
| CN206751785U (en) * | 2016-11-09 | 2017-12-15 | 福建师范大学 | A kind of diatom cultivates plate-type reactor |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP3585876A4 (en) * | 2017-02-23 | 2020-12-23 | Algaennovation Ltd | System and method for growing algae |
| US11629327B2 (en) | 2017-02-23 | 2023-04-18 | Vaxa Technologies Ltd | System and method for growing algae |
| CN107603875A (en) * | 2017-10-30 | 2018-01-19 | 乐山晟嘉电气股份有限公司 | A kind of intelligent biological bacterium culture machine and cultural method |
| CN109355190A (en) * | 2018-10-31 | 2019-02-19 | 北海生巴达生物科技有限公司 | A kind of method that microalgae recycling increases dissolved oxygen with water |
| CN110257221A (en) * | 2019-06-24 | 2019-09-20 | 王晨 | A kind of microalgae bioreactor purifying air and the sunshading board based on the microalgae bioreactor |
| CN110616139A (en) * | 2019-10-15 | 2019-12-27 | 青岛融智汇海洋生物科技股份有限公司 | Process and system for industrially producing biological silicon |
| CN110616139B (en) * | 2019-10-15 | 2020-08-28 | 青岛融智汇海洋生物科技股份有限公司 | Process and system for industrially producing biological silicon |
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Application publication date: 20170222 |
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