CN117044508A - Method for improving rooting effect of rose cutting difficult to root - Google Patents
Method for improving rooting effect of rose cutting difficult to root Download PDFInfo
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Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
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- Life Sciences & Earth Sciences (AREA)
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- Biodiversity & Conservation Biology (AREA)
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- Forests & Forestry (AREA)
- Developmental Biology & Embryology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
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- Chemical & Material Sciences (AREA)
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- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The application discloses a method for improving rooting effect of rose cutting difficult to root, which comprises the following steps: collecting semi-lignified branches of the roses difficult to root, carrying out green branch cutting, and cleaning and shearing the semi-lignified branches to serve as cutting slips; sterilizing the cutting slips, soaking the cutting slips in a growth hormone solution, and obliquely inserting the soaked cutting slips into a matrix for cutting; and spraying a fulvic acid solution on the leaf surfaces of the cutting slips after cutting for post-cutting management, wherein the temperature is 30-35 ℃ and the humidity is more than or equal to 80% in the post-cutting management period, and the growth hormone solution is melatonin solution or a mixed solution of melatonin and indolebutyric acid. According to the method, the combination of the melatonin solution soaked in the cutting slips before cutting and the fulvic acid solution sprayed on the leaf surfaces after cutting is adopted, so that the cutting rooting effect of the roses difficult to root is effectively improved.
Description
Technical Field
The application relates to the technical field of plant seedling raising, in particular to a method for improving the rooting effect of rose cutting difficult to root.
Background
Rose (Rosa rugosa) is a functional flower which is enjoyed internationally, has aromatic flower fragrance and rich nutrition, and is widely used for producing and processing products such as essential oil, cosmetics, foods, tea, traditional Chinese medicines and the like. The rose is also an ornamental plant, integrates excellent properties of cold resistance, drought resistance, disease and pest resistance, salt and alkali resistance and the like, and has great garden application potential. But most rose germplasm has poor cuttage rooting capacity except for a few varieties such as 'purple branch' rose, and the cuttage rooting rate is only 30-50%. At present, the propagation mode adopted by the roses which are difficult to root is mainly grafting, but the defects of low propagation efficiency and high production cost exist, and excellent characters such as saline-alkali tolerance and the like are easily lost. Therefore, a technology capable of improving the rooting effect of the rose cutting difficult to root is developed, and the method has important significance for accelerating the industrialization process of the rose.
In the existing cutting rooting technology, the selection of hormone types and concentrations is the most critical link. The conventional method mostly adopts the hormones such as ABT rooting powder, indolebutyric acid (IBA), naphthalene Acetic Acid (NAA) and the like with different concentrations for soaking or quick dipping. The previous study results confirm that these conventional methods are carried out in Damascus rose @Rosa damascna) And French Qianlike RoseRosa centifolia) The rooting promoting effect can be well achieved on species with easy cuttage rooting.
But the rose of China original originRosa rugosa) Is another species of rose different from the above species, also called "rose-leaf", and is numerous in variety or germplasm, and the cutting rooting ability of different varieties or germplasm is very different. At present, the research of the cutting rooting technology of the wrinkled leaf roses is mainly focused on the variety purple branch roses with easiest cutting rooting, and the rooting rate of the roses can reach more than 90% by adopting a conventional technology. However, for more other varieties or germplasm which are difficult to root, the cutting rooting effect is difficult to be further improved by adopting the conventional technology.
Therefore, aiming at the problem of low rooting rate of rose (Rosa rugosa) cutting seedling raising cutting slips in the prior art, a set of method capable of further improving the rooting effect of rose cutting difficult to root is needed to be developed so as to improve the rooting effect of rose cutting difficult to root and accelerate the industrialization process of rose.
Disclosure of Invention
Aiming at the prior art, the application aims to provide a method for improving the rooting effect of rose cutting difficult to root. According to the method, melatonin (MT) is used for soaking cutting slips before cutting, and fulvic acid is used for spraying the leaf surfaces of the cutting slips periodically after cutting, so that the cutting rooting effect of rose cutting slips difficult to root is improved.
In order to achieve the above purpose, the application adopts the following technical scheme:
the application provides a method for improving rooting effect of rose cutting difficult to root, which comprises the following steps:
(1) Collecting semi-lignified branches of roses difficult to root, cleaning, and shearing to obtain cutting slips; disinfecting the cutting slips, and soaking the disinfected cutting slips in a growth hormone solution;
(2) Inserting the cutting slips soaked by the growth hormone into a matrix;
(3) And spraying fulvic acid solution on the leaf surfaces of the cutting slips for post-cutting management.
Preferably, in the step (1), the variety of the roses difficult to root is 'more Ji Huangmei', and the fine variety number is Lu S-SV-RRX-030-2019.
Preferably, in step (1), the washing time is 10 minutes.
Preferably, in step (1), the shearing treatment is: the half lignified branches of the cleaned roses which are difficult to root are sheared into 10-12 cm long, 2-3 axillary buds are contained, 3-4 compound leaves are reserved at the top, 4-6 small leaves are reserved on each compound leaf, the upper ends of the compound leaves are flatly sheared at the position 0.5cm away from the axillary buds, and the lower end cuts are obliquely sheared.
Preferably, in the step (1), the disinfecting cutting process is as follows: diluting carbendazim with the mass fraction of 80% by 1000 times to obtain a disinfectant, and immersing the cutting slips in the disinfectant for 3-8 min.
Preferably, in the step (1), the lower end incision of the cutting pin after disinfection is immersed in the melatonin solution.
Preferably, in the step (1), the growth hormone solution is melatonin or a mixed solution composed of melatonin and indolebutyric acid.
Further preferably, the concentration of the melatonin solution is 200 mg/L-500 mg/L, and the soaking time is 5 min-60 min;
the concentration of the melatonin and the indolebutyric acid in the mixed solution is the same, the volume ratio of the melatonin to the indolebutyric acid is 1:1, and the concentration of the indolebutyric acid and the melatonin is 200 mg/L-500 mg/L.
Further preferably, the concentration of the melatonin solution is 300mg/L and the soaking time is 60min
Preferably, in step (2), the matrix is perlite.
Preferably, in the step (2), the cutting depth is 4 cm-5 cm, and the plant row spacing is 5cm×5cm.
Preferably, in the step (3), the concentration of the fulvic acid solution is 15mg/L.
Preferably, in the step (3), the temperature is 30-35 ℃ and the humidity is more than or equal to 80% in the management process after cutting.
Preferably, in the step (3), the spraying time is: spraying the leaf surfaces of the cutting slips on the same day after cutting, and spraying once every 7 d; the spraying amount is based on leaf surface dripping.
The application has the beneficial effects that:
in order to improve the rooting rate of the rose cutting difficult to root, the cutting slips are placed in the melatonin solution for soaking treatment before cutting, and the fulvic acid solution is sprayed on leaf surfaces regularly after cutting, so that the rooting effect of the rose cutting difficult to root can be effectively improved. According to the application, through a gradient test, when the cutting slips are soaked in 300mg/L melatonin solution for 60min before cutting, and 15mg/L fulvic acid solution is sprayed on leaf surfaces regularly after cutting, the rooting rate of the roses difficult to root can be improved to 91%, and the root effect index can reach 5.17.
Detailed Description
It should be noted that the following detailed description is illustrative and is intended to provide further explanation of the application. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this application belongs.
As described in the background art, the rooting capacity of the cuttage of the germplasm difficult to root is poor, the rooting capacity of the cuttage is only 30-50%, and in the prior art, most of the cuttage is propagated in a grafting mode, but the propagation efficiency of grafting is low, the cost is high, and the industrialized development of roses is not facilitated.
In the prior art, cutting propagation is usually carried out on Damascus III rose (Rosa×Damascena), 'Hetian' rose (Rosa damascna) and France thousand leaf rose (Rosa centifolia), wherein the Damascus rose, the 'Hetian' rose and the France thousand leaf rose are plants of the same genus and different species as the difficult-to-root rose of the application, and the Damascus rose, the 'Hetian' rose and the France thousand leaf rose belong to plants with very easy cutting propagation, so rooting can be realized only by soaking with a general reagent, namely auxin ABT 1.
The 'Duo Ji Huangmei' roses adopted in the application are the forest fine variety directory (first lot) recommended by the Shandong province preferentially, and the variety characteristics are as follows: the plant height is 180-210cm, and the crown width is 150-180cm; the petals are bright green and yellow, and single petals are formed; flowering period of 4-9 months, and continuous flowering; the flower diameter is 6.5-7cm. When only ABT1 is adopted to soak the plant, the cutting rooting effect is poor. Therefore, the application is researched aiming at the rose variety which is 'up Ji Huangmei' and is difficult to root.
Based on the method, in order to improve the rooting effect of the rose difficult to root in the cutting propagation process, the cutting slip is soaked in a melatonin solution or a mixed solution consisting of the melatonin solution and the indoleacetic acid before cutting, the fulvic acid is sprayed on leaf surfaces after cutting, the influence of the melatonin concentration, the soaking time, the fulvic acid and the like on the rooting effect of the rose difficult to root is researched by adopting a gradient test, and the melatonin concentration, the soaking time and the fulvic acid which are most suitable for rooting of the rose difficult to root in cutting are screened on the basis of two indexes of comprehensive cutting rooting rate and root effect index.
Melatonin (MT) is widely focused as a novel plant growth regulating factor, but the existing research is mainly focused on the influence of melatonin on rooting of seedlings, and the influence on rooting of cutting seedlings is still reported. In principle, melatonin promotes rooting of seedlings, mainly promotes root elongation and lateral root generation, and is further strengthening under the condition that the original root system is generated, and the melatonin acts after the root system is generated. On the promotion of cutting rooting, the callus needs to be dedifferentiated firstly and then dedifferentiated, which belongs to the occurrence of adventitious roots and acts before the root system occurs, and the method is different from the rooting of seedlings in nature. Meanwhile, the cutting rooting is different from the rooting of the tissue culture seedling, the quality of the rooting part of the tissue culture seedling and the quality of the rooting part of the cutting slip are greatly different, the rooting part of the tissue culture seedling is nonlignified, and the rooting part of the cutting slip is semi-lignified. Different textures can lead to different internal physiological conditions, and the types and the concentrations of the applicable rooting hormones are naturally different. Generally, the cutting rooting difficulty is much greater than the tissue culture rooting difficulty.
And (3) spraying a fulvic acid solution on the leaves of the roses difficult to root for carrying out cuttage and then carrying out management, wherein the fulvic acid promotes the growth of plant roots by regulating activities of auxin, gibberellin, cytokinin and the like, reduces stomatal conductance, reduces water transpiration and loss, increases chlorophyll production and promotes photosynthesis so as to promote the cuttage rooting of the roses difficult to root.
In conclusion, the application combines the soaking of cutting slips in melatonin solution or mixed solution composed of melatonin solution and indoleacetic acid before cutting and the spraying of fulvic acid on leaf surfaces after cutting, and has remarkable synergistic effect in improving root system effect index of rose cutting slips difficult to root.
In order to make the technical solution of the present application more clearly understood by those skilled in the art, the technical solution of the present application will be described in detail with reference to specific embodiments.
The test materials used in the examples of the present application are all conventional in the art and are commercially available. Wherein:
the 'Duo Ji Huangmei' is an approved forest fine variety in Shandong province, and the fine variety number is Lu S-SV-RRX-030-2019.
Example 1: method for improving rooting effect of rose cutting difficult to root
(1) In the middle ten days of 6 months, collecting the semi-lignified branches of which the roses 'are more Ji Huangmei' which are difficult to root and grow robustly and have no plant diseases and insect pests, placing the semi-lignified branches in a water-cleaning bucket, and immediately conveying the semi-lignified branches to a forest test station cuttage shed of Shandong agricultural university;
and (5) washing the collected rose semi-lignified branches difficult to root in clear water for 10min to remove surface dust. Then the half lignified branch of the cleaned rose which is difficult to root is sheared into 10cm to 12cm in length, contains 2 axillary buds to 3 axillary buds, keeps 3 to 4 compound leaves at the top, keeps 4 to 6 small leaves of each compound leaf, and cuts the upper end of the compound leaf at a position 0.5cm away from the axillary buds in a flat way, and cuts the lower end of the compound leaf obliquely. And (3) taking the sheared semi-lignified branches as cutting slips, diluting the carbendazim with the mass fraction of 80% by 1000 times to obtain a disinfectant, immersing the cutting slips in the disinfectant for 3-8 min, bundling every 20 sterilized cutting slips into a bundle, enabling the lower end cut to face downwards, and vertically immersing in a growth hormone solution. Wherein the growth hormone solution is 300mg/L melatonin solution, and the soaking time is 60min;
(2) The soaked cutting is inserted into the small particle perlite matrix, and the surrounding matrix is compacted. Wherein, the cutting depth is 4-5 cm, and the spacing between plant rows is 5cm multiplied by 5cm;
(3) And carrying out post-cutting management on the cutting slips after cutting. And (3) periodically spraying 15mg/L fulvic acid solution on the leaf surfaces of the cutting slips, wherein the spraying time is 0d, 7d, 14d and 21d after cutting, and the spraying amount is based on leaf surface water dripping. And during the management period after cutting, a full-automatic spraying system is adopted to adjust the temperature and the air humidity in the cutting shed, so that the temperature is kept at 30-35 ℃ and the air humidity is kept above 80%.
Example 2: method for improving rooting effect of rose cutting difficult to root
This embodiment differs from embodiment 1 in that: in the step (1), the growth hormone solution is melatonin of 200mg/L.
Example 3: method for improving rooting effect of rose cutting difficult to root
This embodiment differs from embodiment 1 in that: in the step (1), the growth hormone solution is 400mg/L melatonin, and the soaking time is 30min.
Example 4: method for improving rooting effect of rose cutting difficult to root
This embodiment differs from embodiment 1 in that: in the step (1), the growth hormone solution is melatonin of 500mg/L, and the soaking time is 5min.
Example 5: method for improving rooting effect of rose cutting difficult to root
This embodiment differs from embodiment 1 in that; in the step (1), the growth hormone solution is a mixed solution consisting of melatonin and indolebutyric acid, the soaking time is 60min, wherein the volume ratio of the melatonin to the indolebutyric acid in the mixed solution is 1:1, and the concentration of the melatonin and the indolebutyric acid is 200mg/L.
Example 6: method for improving rooting effect of rose cutting difficult to root
This embodiment differs from embodiment 1 in that: in the step (1), the growth hormone solution is a mixed solution consisting of melatonin and indolebutyric acid, the soaking time is 60min, wherein the volume ratio of the melatonin to the indolebutyric acid in the mixed solution is 1:1, and the concentration of the melatonin and the indolebutyric acid is 300mg/L.
Example 7: method for improving rooting effect of rose cutting difficult to root
This embodiment differs from embodiment 1 in that: in the step (1), the growth hormone solution is a mixed solution consisting of melatonin and indolebutyric acid, and the soaking time is 30min, wherein the volume ratio of the melatonin to the indolebutyric acid in the mixed solution is 1:1, and the concentration of the melatonin and the indolebutyric acid is 400mg/L.
Example 8: method for improving rooting effect of rose cutting difficult to root
This embodiment differs from embodiment 1 in that: in the step (1), the growth hormone solution is a mixed solution consisting of melatonin and indolebutyric acid, the soaking time is 5min, wherein the volume ratio of the melatonin to the indolebutyric acid in the mixed solution is 1:1, and the concentration of the melatonin and the indolebutyric acid is 500mg/L.
Comparative example 1: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 1 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 2: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 2 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 3: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 3 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 4: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 4 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 5: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 5 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 6: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 6 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 7: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 7 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 8: method for improving rooting effect of rose cutting difficult to root
The difference between this comparative example and example 8 is that: in the step (3), clear water is sprayed on the leaf surfaces of the cutting slips in the post-cutting management period.
Comparative example 9:
the difference between this comparative example and example 1 is that: the growth hormone solution was replaced with clean water.
Test example:
rooting rate and root effect index of cuttings in examples 1 to 8 and comparative examples 1 to 9 were counted at 28 days after cutting, and the results are shown in table 1.
Wherein, the number of the cutting slips in the examples 1 to 8 and the comparative examples 1 to 9 is 100;
root effect index= (average root length x root number)/total cutting number.
TABLE 1 rooting Rate and root System Effect index
As can be seen from Table 1, the rooting rate of rose cutting treated by melatonin before cutting can reach 91% and the root effect index of rose cutting treated by mixed solution of melatonin and indoleacetic acid before cutting can reach 86% and the root effect index of rose cutting treated by melatonin before cutting can reach 4.48, and the rooting rate and root effect index of rose cutting treated by melatonin before cutting are higher than those of mixed treatment of melatonin and IBA at the same concentration. Meanwhile, according to the prior art, the rose cutting rooting rate and root effect index which are treated by melatonin are higher than those of the rose which is treated by indolebutyric acid (IBA) with the same concentration. The root effect index is related to the rooting quantity and the average root length, and the product of the root effect index and the average root length is used for representing the root effect in a cutting test, and the larger the root effect index is, the better the root effect is. From this, it can be seen that melatonin has better effect of promoting rooting of rose cutting difficult to root than indolebutyric acid (IBA). In addition, it can be seen that the long-term soaking effect of the low-concentration melatonin is better than that of the high-concentration melatonin.
During the management period after cutting, the rooting rate and root effect index of rose cutting by periodically spraying fulvic acid aqueous solution on leaf surfaces are higher than those of the treatment by periodically spraying clear water. Therefore, the cutting rooting effect of the roses difficult to root can be further improved by spraying the fulvic acid aqueous solution on the leaf surfaces at regular intervals.
Meanwhile, compared with the example 1, the comparative example 1 adopts melatonin to soak cutting slips before cutting and uses clear water to regularly spray rose leaves after cutting, the rooting rate of rose cutting is only 86%, and the root effect index is only 3.85; comparative example 9 the cutting was soaked with clear water before cutting and the rose leaves were sprayed periodically with fulvic acid after cutting, the rooting rate of rose cutting was only 38% and the root system effect index was only 0.81. Therefore, the application combines the melatonin soaking cutting slips and the regular spraying of the humic acid on the rose leaves after cutting, and can obviously improve the cutting rooting effect of the rose cutting slips difficult to root.
In conclusion, the melatonin soaked cutting and the timed foliar spray fulvic acid aqueous solution are combined for use, so that the cutting rooting effect of the roses difficult to root can be effectively improved. Wherein 300 mg.L -1 Melatonin soaked cutting slips for 60min and 15 mg.L sprayed on regular leaf surfaces -1 The treatment effect of the fulvic acid aqueous solution is best, the rooting rate of the roses difficult to root can be improved to 91%, and the root system effect index reaches 5.17.
The above description is only of the preferred embodiments of the present application and is not intended to limit the present application, but various modifications and variations can be made to the present application by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present application should be included in the protection scope of the present application.
Claims (9)
1. The method for improving the rooting effect of the rose cutting difficult to root is characterized by comprising the following steps of:
(1) Collecting semi-lignified branches of roses difficult to root, cleaning, and shearing to obtain cutting slips; disinfecting the cutting slips, and soaking the disinfected cutting slips in a growth hormone solution;
(2) Inserting the cutting slips soaked by the growth hormone into a matrix;
(3) Spraying fulvic acid solution on the leaf surfaces of the cutting slips for post-cutting management;
the growth hormone solution is melatonin solution or mixed solution composed of melatonin and indolebutyric acid.
2. The method for improving rooting effect of rose cutting with difficult rooting according to claim 1, wherein in the step (1), the rose with difficult rooting is 'Duo Ji Huangmei', and the improved variety number is Lu S-SV-RRX-030-2019.
3. The method for improving rooting effect of rose cutting with difficult rooting according to claim 1, wherein in the step (1), the shearing treatment is as follows: the half lignified branches of the cleaned roses which are difficult to root are sheared into 10-12 cm long, 2-3 axillary buds are contained, 3-4 compound leaves are reserved at the top, 4-6 small leaves are reserved on each compound leaf, the upper ends of the compound leaves are flatly sheared at the position 0.5cm away from the axillary buds, and the lower end cuts are obliquely sheared.
4. The method for improving the rooting effect of the rose cutting difficult to root according to claim 1, wherein in the step (1), the disinfection cutting is performed by: diluting carbendazim with the mass fraction of 80% by 1000 times to obtain a disinfectant, and immersing the cutting slips in the disinfectant for 3-8 min.
5. The method for improving the rooting effect of the rose cutting difficult to root according to claim 1, wherein the concentration of the melatonin solution is 200-500 mg/L and the soaking time is 5-60 min;
the concentration of the melatonin and the indolebutyric acid in the mixed solution is the same, the volume ratio of the melatonin to the indolebutyric acid is 1:1, and the concentration of the indolebutyric acid and the melatonin is 200 mg/L-500 mg/L.
6. The method for improving rooting effect of rose cutting with difficult rooting according to claim 1, wherein in the step (2), the cutting depth is 4-5 cm, and the plant row spacing is 5cm×5cm.
7. The method for improving the rooting effect of the rose cutting with difficult rooting according to claim 1, wherein in the step (3), the temperature is 30-35 ℃ and the humidity is more than or equal to 80% in the post-cutting management process.
8. The method for improving the rooting effect of the cutting of the roses difficult to root according to claim 1, wherein in the step (3), the concentration of the fulvic acid solution is 15mg/L.
9. The method for improving the rooting effect of the rose cutting difficult to root according to claim 1, wherein in the step (3), the spraying time is as follows: spraying the leaf surfaces of the cutting slips on the same day after cutting, and spraying once every 7 d; the spraying amount is based on leaf surface dripping.
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