CN117016569A - Microbial composition for preventing and treating plant leaf fungal diseases and application thereof - Google Patents
Microbial composition for preventing and treating plant leaf fungal diseases and application thereof Download PDFInfo
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- CN117016569A CN117016569A CN202311287318.8A CN202311287318A CN117016569A CN 117016569 A CN117016569 A CN 117016569A CN 202311287318 A CN202311287318 A CN 202311287318A CN 117016569 A CN117016569 A CN 117016569A
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- 239000000203 mixture Substances 0.000 title claims abstract description 79
- 208000031888 Mycoses Diseases 0.000 title claims abstract description 17
- 230000000813 microbial effect Effects 0.000 title claims abstract description 15
- 241000196324 Embryophyta Species 0.000 claims abstract description 91
- 241000193830 Bacillus <bacterium> Species 0.000 claims abstract description 34
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 28
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims abstract description 19
- 240000008042 Zea mays Species 0.000 claims abstract description 19
- 235000007244 Zea mays Nutrition 0.000 claims abstract description 19
- 229940089639 cornsilk Drugs 0.000 claims abstract description 19
- 150000004676 glycans Chemical class 0.000 claims abstract description 19
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 19
- 239000005017 polysaccharide Substances 0.000 claims abstract description 19
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- WSMYVTOQOOLQHP-UHFFFAOYSA-N Malondialdehyde Chemical compound O=CCC=O WSMYVTOQOOLQHP-UHFFFAOYSA-N 0.000 claims abstract description 15
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- 239000005819 Potassium phosphonate Substances 0.000 claims abstract description 10
- YXXXKCDYKKSZHL-UHFFFAOYSA-M dipotassium;dioxido(oxo)phosphanium Chemical compound [K+].[K+].[O-][P+]([O-])=O YXXXKCDYKKSZHL-UHFFFAOYSA-M 0.000 claims abstract description 10
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- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 claims description 17
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- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims description 8
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims description 8
- 239000002509 fulvic acid Substances 0.000 claims description 8
- 229940095100 fulvic acid Drugs 0.000 claims description 8
- 244000052769 pathogen Species 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 6
- 238000005507 spraying Methods 0.000 claims description 6
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 claims description 5
- 239000004021 humic acid Substances 0.000 claims description 5
- XEGMDUOAESTQCC-UHFFFAOYSA-N 1-(naphthalen-1-ylmethyl)naphthalene;sodium Chemical compound [Na].C1=CC=C2C(CC=3C4=CC=CC=C4C=CC=3)=CC=CC2=C1 XEGMDUOAESTQCC-UHFFFAOYSA-N 0.000 claims 1
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- ATNHDLDRLWWWCB-AENOIHSZSA-M chlorophyll a Chemical compound C1([C@@H](C(=O)OC)C(=O)C2=C3C)=C2N2C3=CC(C(CC)=C3C)=[N+]4C3=CC3=C(C=C)C(C)=C5N3[Mg-2]42[N+]2=C1[C@@H](CCC(=O)OC\C=C(/C)CCC[C@H](C)CCC[C@H](C)CCCC(C)C)[C@H](C)C2=C5 ATNHDLDRLWWWCB-AENOIHSZSA-M 0.000 abstract description 14
- 102000004190 Enzymes Human genes 0.000 abstract description 10
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- 230000012010 growth Effects 0.000 description 13
- 239000003795 chemical substances by application Substances 0.000 description 11
- 230000001276 controlling effect Effects 0.000 description 11
- 239000003337 fertilizer Substances 0.000 description 11
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 8
- 239000002994 raw material Substances 0.000 description 8
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- 229910052708 sodium Inorganic materials 0.000 description 8
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- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 7
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- 238000012360 testing method Methods 0.000 description 4
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- 102000016938 Catalase Human genes 0.000 description 3
- 108010053835 Catalase Proteins 0.000 description 3
- 102000030523 Catechol oxidase Human genes 0.000 description 3
- 108010031396 Catechol oxidase Proteins 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 102000019197 Superoxide Dismutase Human genes 0.000 description 3
- 108010012715 Superoxide dismutase Proteins 0.000 description 3
- 230000002411 adverse Effects 0.000 description 3
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- 238000012258 culturing Methods 0.000 description 3
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
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- 102000003992 Peroxidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 239000002671 adjuvant Substances 0.000 description 1
- 238000012271 agricultural production Methods 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000011609 ammonium molybdate Substances 0.000 description 1
- APUPEJJSWDHEBO-UHFFFAOYSA-P ammonium molybdate Chemical compound [NH4+].[NH4+].[O-][Mo]([O-])(=O)=O APUPEJJSWDHEBO-UHFFFAOYSA-P 0.000 description 1
- 229940010552 ammonium molybdate Drugs 0.000 description 1
- 235000018660 ammonium molybdate Nutrition 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 238000003321 atomic absorption spectrophotometry Methods 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000003124 biologic agent Substances 0.000 description 1
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- 230000003247 decreasing effect Effects 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
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- 230000000877 morphologic effect Effects 0.000 description 1
- 231100000350 mutagenesis Toxicity 0.000 description 1
- LGROKZMEHJZWDU-UHFFFAOYSA-N n-amino-n-phenylnitramide Chemical compound [O-][N+](=O)N(N)C1=CC=CC=C1 LGROKZMEHJZWDU-UHFFFAOYSA-N 0.000 description 1
- FEMOMIGRRWSMCU-UHFFFAOYSA-N ninhydrin Chemical compound C1=CC=C2C(=O)C(O)(O)C(=O)C2=C1 FEMOMIGRRWSMCU-UHFFFAOYSA-N 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000004806 packaging method and process Methods 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 239000000546 pharmaceutical excipient Substances 0.000 description 1
- OQUKIQWCVTZJAF-UHFFFAOYSA-N phenol;sulfuric acid Chemical compound OS(O)(=O)=O.OC1=CC=CC=C1 OQUKIQWCVTZJAF-UHFFFAOYSA-N 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 239000003223 protective agent Substances 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- FQENQNTWSFEDLI-UHFFFAOYSA-J sodium diphosphate Chemical compound [Na+].[Na+].[Na+].[Na+].[O-]P([O-])(=O)OP([O-])([O-])=O FQENQNTWSFEDLI-UHFFFAOYSA-J 0.000 description 1
- 229940048086 sodium pyrophosphate Drugs 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
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- 230000009885 systemic effect Effects 0.000 description 1
- 235000019818 tetrasodium diphosphate Nutrition 0.000 description 1
- 239000001577 tetrasodium phosphonato phosphate Substances 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/44—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a nitrogen atom attached to the same carbon skeleton by a single or double bond, this nitrogen atom not being a member of a derivative or of a thio analogue of a carboxylic group, e.g. amino-carboxylic acids
- A01N37/46—N-acyl derivatives
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/02—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms
- A01N43/04—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom
- A01N43/14—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings
- A01N43/16—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having rings with one or more oxygen or sulfur atoms as the only ring hetero atoms with one hetero atom six-membered rings with oxygen as the ring hetero atom
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N61/00—Biocides, pest repellants or attractants, or plant growth regulators containing substances of unknown or undetermined composition, e.g. substances characterised only by the mode of action
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Chemical & Material Sciences (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Inorganic Chemistry (AREA)
- Mycology (AREA)
- Toxicology (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to a microbial composition for preventing and treating plant leaf fungal diseases and application thereof. The composition provided by the invention comprises the bacillus siamensis and/or the microbial inoculum thereof and corn silk polysaccharide, biochemical potassium fulvate, polyglutamic acid, potassium phosphite and other production byproducts or waste, and through the cooperation of the components, the stress resistance of plants can be improved, the defensive enzyme activity in plant leaves can be improved, the content of toxic substances (malondialdehyde) in plant cells can be reduced, the accumulation of crop leaf nutrient substances can be promoted, the chlorophyll content in the leaves can be improved, and the composition has good prevention and control effects on various plant fungal diseases. After the Siamese bacillus provided by the invention is added into the composition, the effect is further improved.
Description
Technical Field
The invention belongs to the technical field of microorganisms and organic fertilizers, and particularly relates to a microorganism composition for preventing and treating plant leaf fungal diseases and application thereof.
Background
China has abundant Chinese medicinal material resources and has the natural advantage of developing the strong Chinese medicinal industry. According to statistics of Chinese medicine institute of Chinese medical science and academy, chinese medicinal materials are classified according to sources, and can be divided into 3 kinds of medicinal plants, medicinal animals and medicinal minerals, namely 11146 kinds, 1581 kinds and 80 kinds. However, the development and production of the traditional Chinese medicine industry are generally limited by various diseases, and the growth of traditional Chinese medicine plants is greatly influenced, so that the income of farmers and the development of economy are adversely affected.
At present, most of crop diseases are promoted to grow and the occurrence of the diseases is restrained by using chemical agriculture and chemical fertilizers in production, but the long-term large-scale use of chemical pesticides and fertilizers can bring irreversible harm, such as land fertility reduction, soil nutrient loss, environmental pollution and the like, and meanwhile, the unscientific medication can also cause the problems of increased drug resistance of pathogenic microorganisms and the like, so that the disease epidemic speed is generally increased and the prevention and control are more difficult. The biological control method has the characteristics of green and sustainable, has specific inhibition activity on plant pathogenic microorganisms, and does not generate drug resistance on the plant pathogenic microorganisms, so that the effective control of diseases by antagonistic microorganisms is a more efficient and environment-friendly effective measure in a green agricultural background, and has positive effects of promoting crop growth, improving crop yield and preventing and controlling diseases.
Disclosure of Invention
The invention aims to provide a microbial composition for preventing and treating plant leaf fungal diseases and application thereof, aiming at the problems of poor effect, high environmental protection risk and the like of chemical fertilizers and pesticides in the traditional Chinese medicinal material planting process in the prior art. The composition provided by the invention has good antagonism on gentiana macrophylla leaf rust bacteria, paris polyphylla gray mold bacteria, paris polyphylla leaf spot bacteria and polygonatum kingianum leaf spot bacteria, has the effect of high-efficiency nitrogen fixation, and can improve the promotion effects of disease resistance and production performance of Chinese medicinal plants.
To achieve the above object, the present invention provides, in one aspect: a microbial composition for controlling fungal diseases of plant leaves, the composition comprising the following components:
component 1: the preservation number is CCTCC NO: m2022925, and/or a microbial inoculum thereof;
component 2: corn silk polysaccharide, biochemical potassium fulvate and polyglutamic acid;
and (3) a component 3: potassium phosphite.
Further, the amount of component 1 in the composition is 0.1 to 2% by weight and the amount of component 3 is 3 to 8% by weight based on the total weight; the weight ratio of the corn silk polysaccharide to the biochemical potassium fulvate to the polyglutamic acid in the component 2 is 1-5:70-80:1-5.
Further, the microbial inoculum comprises Siamese bacillus and auxiliary materials, wherein the auxiliary materials are methylene dinaphthyl sodium sulfonate (NNO) and polysiloxane in a weight ratio of 0.5-2:1-3, and the whole auxiliary materials account for 2-8% of the total weight of the microbial inoculum.
Further, the composition contains Siamella and/or the microbial inoculum in an amount such that the content of Siamella in the composition is 1×10 10 -3×10 13 cfu/g。
Further, wherein the corn silk polysaccharide has a moisture content of 30-40 wt%;
and/or the biochemical fulvic acid content in the biochemical fulvic acid potassium is 30-35 wt%, the humic acid content is 2-8 wt% and the K is calculated by dry matter 2 The O content is 5-15 wt%;
and/or the weight average molecular weight of the polyglutamic acid is 1 ten thousand to 1.5 ten thousand.
Further, the corn silk polysaccharide has a sugar content of 90-95 wt% and other substances of 5-10 wt% based on dry matter.
In a second aspect, the invention provides the use of a composition for reducing malondialdehyde content in plant leaves, increasing plant system resistance, and/or promoting plant growth, and/or controlling plant diseases.
In a third aspect the present invention provides a method of promoting plant growth and/or controlling plant disease, the method comprising spraying onto the foliage of a plant a composition according to any of the preceding.
Further, the plant diseases are gentiana macrophylla leaf rust, paris polyphylla gray mold, paris polyphylla leaf spot and polygonatum kingianum leaf spot; wherein the pathogen of the gentiana macrophylla leaf rust is Ma Geshan rust bacteria @Melampsoramagnusiana Wagner) The pathogenic agent of gray mold of rhizoma paridis is BotrytisBotrytis sinoviticolaThe pathogenic factor of Paris polyphylla leaf spot is cercosporaPestalotiopsisoryzae) The pathogen of the leaf spot disease of the Polygonatum kingianum is Spot mould of the first partyPhyllostictacapitalensis) The method comprises the steps of carrying out a first treatment on the surface of the And/or the use amount of the Siamese bacillus, the microbial inoculum or the composition is such that the use amount of the Siamese bacillus applied to the plant leaf surface is not less than 5 multiplied by 10 10 cfu/mu/time, and/or the usage amount of the composition for plant leaf surface is 300-600 g/mu/time.
Advancing oneThe use amount of the Siamese bacillus, the microbial inoculum or the composition is that the use amount of the Siamese bacillus applied to the plant leaf surface is 1 multiplied by 10 12 -5×10 13 cfu/mu/time, the application frequency of the composition is 1-3 times per year.
The invention has the beneficial effects that:
(1) The Siamese bacillus SH-1439 provided by the invention has high-efficiency antagonistic capability on various plant pathogenic fungi. When applied to plant leaf surfaces, the strain not only can improve the chlorophyll content of the plant leaf surfaces, but also can improve the growth rate of plants. In addition, the strain can effectively reduce the content of malondialdehyde in plant leaves, improve the defensive enzyme activity of the plant leaves, and has good control effect on plant leaf fungal diseases.
(2) The Siamese bacillus SH-1439 provided by the invention is plant rhizosphere bacteria separated from tobacco plant rhizosphere soil, and compared with a strain artificially cultivated in a plurality of modes such as genetic engineering improvement or induced mutation in the prior art, the Siamese bacillus SH-1439 has better colonial capacity and is more beneficial to continuously playing a role after application.
(3) The composition provided by the invention can effectively reduce the content of malondialdehyde in plant leaves, improve the chlorophyll content of the plant leaves and promote the growth of crops. Further researches show that the composition has good promoting effect on the activity of plant leaf surface defensive enzymes, can improve the systemic resistance of plants, and can effectively solve the problems that the existing plant growth promoting effect is poor or continuous growth promotion cannot be realized.
(4) The composition provided by the invention takes corn silk polysaccharide, biochemical potassium fulvate, polyglutamic acid, potassium phosphite and other production byproducts (waste) as main raw materials, and has the characteristics of wide raw material sources, low cost, green and environment-friendly performance and simple preparation method. Meanwhile, the production and application of the composition can promote the recycling of wastes and the sustainable development of agricultural production, is beneficial to promoting the adjustment of an industrial structure, and has positive significance for the improvement of the total agricultural yield and the increase of income of farmers.
Preservation of organisms
The invention provides Siamese bacillusBacillus siamensis) The rice is preserved in China center for type culture collection (China) for 6 months and 20 days in 2022, and has the address of eight paths 299 of Wuchang district of Wuhan, hubei province, university of Wuhan and the preservation number of CCTCC NO: m2022925. The relevant morphological and biochemical characteristics and molecular biological identification results of the strain are as follows: cn202211189773.X is disclosed.
Detailed Description
The endpoints and any values of the ranges disclosed herein are not limited to the precise range or value, and are understood to encompass values approaching those ranges or values. For numerical ranges, one or more new numerical ranges may be found between the endpoints of each range, between the endpoint of each range and the individual point value, and between the individual point value, in combination with each other, and are to be considered as specifically disclosed herein.
In the invention, the Siamese bacillus SH-1439 and the Siamese bacillus CCTCC NO: m2022925 is the same strain, both of which are synonymous, and their names (numbers) are used interchangeably.
The inventor of the invention separates and obtains a bacillus siamensis strain in the research processBacillus siamensis) The product is named SH-1439 and is preserved in China Center for Type Culture Collection (CCTCC) in 2022, 6 and 20 days, wherein the preservation number is CCTCC NO: m2022925. The research shows that the strain has good antagonism to plant pathogenic fungi. Through further research, the inventor skillfully finds that when the bacillus siamensis is applied to the leaf surfaces of plants, the bacillus siamensis can promote the growth of the plants, reduce the malondialdehyde content in the plant leaves, improve the chlorophyll content of the leaf surfaces, improve the activity of plant defensive enzymes and the like, and more permanently improve the resistance of the plants to diseases such as leaf rust, gray mold, leaf spot and the like (especially the leaf diseases caused by fungi), and has better control effect.
Based on the above findings, the present invention provides in one aspect a microbial composition for controlling fungal diseases of plant leaves, the composition comprising the following components:
component 1: the preservation number is CCTCC NO: m2022925, and/or a microbial inoculum thereof;
component 2: corn silk polysaccharide, biochemical potassium fulvate and polyglutamic acid;
and (3) a component 3: potassium phosphite.
In the present invention, the specific formulation of the microbial agent is not particularly limited, and any type of microbial agent commonly used in the art may be applied to the present invention. For example, the bacterial agent may be a solid bacterial agent, a liquid bacterial agent, or a semisolid (concentrated) bacterial agent. The auxiliary materials and the content thereof selected in the microbial inoculum are not particularly limited as long as the auxiliary materials and the content thereof are specific to the bacillus siamensis CCTCC NO: the effect of M2022925 is not adversely affected or is not substantially adversely affected (for example, the effect of the strain after the addition of the auxiliary material is not less than 80% of that when the strain alone is used). Adjuvants commonly used in the art for the preparation of bacterial agents are suitable for use in the present invention, such as protective agents, excipients, binders, fillers, and the like.
Preferably, component 1 is used in an amount of 0.1 to 2% by weight and component 3 is used in an amount of 3 to 8% by weight, based on the total weight of the composition; the weight ratio of the corn silk polysaccharide to the biochemical potassium fulvate to the polyglutamic acid in the component 2 is 1-5:70-80:1-5.
Preferably, the microbial inoculum comprises Siamese bacillus and auxiliary materials, wherein the auxiliary materials comprise sodium methylenedinaphthyl sulfonate (NNO) and polysiloxane in a weight ratio of 0.5-2:1-3, and the whole auxiliary materials account for 2-8% of the total weight of the microbial inoculum. Preferably 3-8 wt%. More preferably 5-8 wt%.
In the present invention, sodium methylenedinaphthyl sulfonate (NNO) and polysiloxane selected as auxiliary materials are not particularly limited, and any reagent commonly used in the art as the above-mentioned microbial inoculum may be applied to the present invention. The specific amounts and proportions of sodium methylenedinaphthyl sulfonate (NNO) and polysiloxane used as auxiliary materials are not particularly limited in the present invention, and may be adjusted accordingly according to actual conditions (e.g., planting conditions, crop growth conditions, growth characteristics, etc.).
Preferably, the composition contains Siamella and/or the microbial inoculum in an amount such that the content of Siamella in the composition is 1×10 10 -3×10 13 cfu/g; preferably 3X 10 10 -3×10 13 cfu/g. More preferably 3X 10 11 -3×10 13 cfu/g 。
Preferably, in the composition, in the component 2, the weight ratio of corn silk polysaccharide, biochemical potassium fulvate and polyglutamic acid is 1-5:70-80:1-5, preferably 2-5:73-80:2-5. In component 3, the weight ratio of potassium phosphite is 3-8%, preferably 5-8%.
In the present invention, the raw materials (such as corn silk polysaccharide, biochemical potassium fulvate, polyglutamic acid, potassium phosphite, etc.) used as the above composition are not particularly limited, and may be any related products that can be used in the art for preparing biological agents, either commercially available related products or related products that are prepared by themselves according to the prior art.
According to a preferred embodiment of the present invention, wherein the corn silk polysaccharide has a moisture content of 30-40 wt%;
and/or the biochemical fulvic acid content in the biochemical fulvic acid potassium is 30-35 wt%, the humic acid content is 2-8 wt% and the K is calculated by dry matter 2 The O content is 5-15 wt%;
and/or the weight average molecular weight of the polyglutamic acid is 1 ten thousand to 1.5 ten thousand.
Preferably, the corn silk polysaccharide has a sugar content of 90-95 wt% and other substances of 5-10 wt% based on dry matter.
The inventor of the present invention also found in the research that when the siamese bacillus is further added into the above composition, the effects of reducing the malondialdehyde content in plant leaves, improving the chlorophyll content in plant leaves, improving the activity of plant leaf defensive enzymes, preventing and controlling leaf fungal diseases and the like can be further improved.
The present invention further provides a method for preparing the above composition, according to a preferred embodiment of the present invention, wherein the method comprises storing the aforementioned Siamese bacillus (or its microbial inoculum) of component 1 separately; uniformly mixing the corn silk polysaccharide, the biochemical potassium fulvate, the polyglutamic acid and other raw materials of the component 2 according to the dosage ratio; separately storing the potassium phosphite of the component 3; mixing the auxiliary materials of sodium methylenedinaphthyl sulfonate (NNO) and polysiloxane uniformly according to the dosage ratio.
In the present invention, there is no particular limitation on the specific storage and use form of the composition. Because the raw materials adopted in the composition are basically solid, the preparation method is only to uniformly mix the components according to the proportion. Thus, the composition can be stored or used directly in the form of a solid preparation obtained. The composition can also be mixed with a certain amount of water to prepare a preparation in the form of liquid or slurry (suspension) for storage or use in consideration of different crops or planting modes and planting conditions.
In a second aspect, the invention provides the use of a composition for reducing malondialdehyde content in plant leaves, increasing plant system resistance, and/or promoting plant growth, and/or controlling plant diseases.
According to a preferred embodiment of the present invention, wherein the plant is a gentian, paris and polygonatum plant. For example, it may be any plant used for production in the art, such as gentiana macrophylla of gentian, paris polyphylla of paris and polygonatum kingianum of polygonatum.
In the present invention, promoting crop growth refers to increasing chlorophyll content in leaves, increasing plant system resistance refers to increasing antioxidant enzyme activity (such as superoxide dismutase, peroxidase, catalase, and polyphenol oxidase) in leaves, and decreasing malondialdehyde content in leaves.
In the present invention, controlling plant diseases means preventing or reducing occurrence of plant diseases, or reducing loss caused by diseases after occurrence of diseases, and the like.
Preferably, the plant disease is selected from foliar diseases. Preferably plant leaf diseases caused by fungi, such as leaf rust of Gentiana macrophylla, gray mold of Paris polyphylla, leaf spot of Paris polyphylla and leaf spot of Polygonatum kingianum. Wherein the pathogen of the gentiana macrophylla leaf rust is Ma Geshan rust bacteria @Melampsoramagnusiana Wagner) The pathogenic agent of gray mold of rhizoma paridis is BotrytisBotrytis sinoviticolaThe pathogenic factor of Paris polyphylla leaf spot is cercosporaPestalotiopsisoryzae) The pathogen of the leaf spot disease of the Polygonatum kingianum is Spot mould of the first partyPhyllostictacapitalensis）。
In a third aspect the present invention provides a method of promoting plant growth and/or controlling plant disease comprising spraying the composition described above onto the foliage of a plant.
In the present invention, the plant is gentian, paris and Polygonatum plants, for example, any plant used in the art for production, such as gentiana macrophylla of gentian, paris and Polygonatum, etc.
Preferably, the plant disease is selected from foliar diseases. Preferably plant leaf diseases caused by fungi, such as leaf rust of Gentiana macrophylla, gray mold of Paris polyphylla, leaf spot of Paris polyphylla and leaf spot of Polygonatum kingianum.
The specific features of the Siamese bacillus, the microbial inoculum and the composition adopted in the method provided by the invention are as described above and are not described in detail herein.
In the invention, the specific dosage of the Siamese bacillus, the microbial inoculum and the composition is not particularly limited, so long as the function of promoting plant growth can be achieved.
According to a preferred embodiment of the present invention, wherein, for the above-mentioned modes (1) and (3), the amount of the Bacillus siamensis, the microbial inoculum or the composition is such that the amount of the Bacillus siamensis applied to the foliage of the plant is not less than 5X 10 10 cfu/mu/time, preferably may be 1X 10 11 -5×10 12 cfu/mu/time. For example, it may be 1X 10 11 cfu/mu/time, 2X 10 11 cfu/mu/time, 3X 10 11 cfu/mu/time, 5X 10 11 cfu/mu/time, 8×10 11 cfu/mu/time, 1X 10 12 cfu/mu/time, 3X 10 12 cfu/mu/time, 5X 10 12 cfu/mu/time, 8×10 12 cfu/mu/time, 1X 10 13 cfu/mu/time, 3X 10 13 cfu/mu/time, 5X 10 13 cfu/mu/time, 8×10 13 cfu/mu/time, 1X 10 14 cfu/mu/time, 2X 10 14 cfu/mu/time, 3X 10 14 cfu/mu/time, 4X 10 14 cfu/mu/time, 5X 10 12 cfu/mu/time, orOr any intermediate value between any two values. The amount of the Siamese bacillus is calculated according to the application amount of the microbial inoculum or the composition and the amount of the Siamese bacillus contained in the microbial inoculum, wherein the liquid microbial inoculum is converted according to 1 g/mL.
The composition provided by the invention has a synergistic effect when being used together with the Siamese bacillus, so that the use amount of the Siamese bacillus in the mode (1) can be appropriately higher than that in the mode (3) in order to obtain a better effect.
According to a preferred embodiment of the present invention, wherein, for the above modes (2) and (3), the composition may be applied to the foliage of the plant in an amount of 300 to 600 g/mu/time.
Preferably, the siamese bacillus, microbial inoculum or composition may be applied 1-3 times per year.
In order to make the objects, technical solutions and advantageous effects of the present invention more apparent, preferred embodiments of the present invention will be described in detail below to facilitate understanding by the skilled person.
Example 1
Strain characterization study
Antagonistic activity assay: the antagonism of Siamese bacillus SH-1439 to Botrytis cinerea, paris polyphylla leaf spot bacteria and Polygonatum kingianum leaf spot bacteria is tested by adopting a flat plate counter method, and the specific experimental process comprises the following steps: the preparation method comprises the steps of taking the botrytis cinerea, the leaf spot bacteria of the paris polyphylla and the leaf spot bacteria of the Polygonatum kingianum as indication pathogens, inoculating 3 mm pathogenic bacteria cakes in the center of a PDA culture medium, inoculating antagonistic strains at a position 25 mm away from the PDA culture medium according to a cross shape, taking an unvaccinated plate as a control, repeating 3 times of each strain, and culturing in a constant-temperature incubator at 25-28 ℃ for 5-7 days, thereby calculating the bacteriostasis rate.
After the completion of the culture, an average inhibition ratio was calculated, inhibition ratio (%) = (control group colony diameter-treatment group colony diameter)/(control group colony diameter-0.3 cm) ×100.
SH-1439 has good inhibition effects on Botrytis cinerea, paris polyphylla leaf spot bacteria and Polygonatum kingianum leaf spot bacteria, and the inhibition rate is calculated to be more than 70%.
Example two
The compositions were formulated according to the formulation in table 2, the specific formulation method comprising: firstly, weighing raw materials according to the proportion shown in the table 2, uniformly mixing the raw materials, and dissolving the raw materials in water to obtain a liquid composition; secondly, weighing auxiliary materials, namely sodium methylenedinaphthyl sulfonate (NNO) and polysiloxane according to the weight ratio of 0.5-2:1-3; and finally, uniformly mixing the auxiliary materials and the formula shown in the table 2.
The corn silk polysaccharide is purchased from Chengdu Sanzhu Kogyang crop science Co., ltd, the biochemical potassium fulvate is purchased from Jinan Xuerui chemical Co., ltd, the polyglutamic acid is purchased from Sian Wan biotechnology Co., ltd, and the potassium phosphite is purchased from Shandong Xinjiang chemical Co., ltd. The Siamese bacillus bacterial agent is a liquid or solid bacterial agent prepared by amplifying and culturing Siamese bacillus SH-1439 and concentrating, wherein the content of the Siamese bacillus is 2 multiplied by 10 12 cfu/g. The auxiliary material is selected from a mixture of sodium methylenedinaphthyl sulfonate (NNO) and polysiloxane, and the dosage is such that the weight ratio of the sodium methylenedinaphthyl sulfonate (NNO) to the polysiloxane in the auxiliary material is 0.5-2:1-3.
The sugar content in the corn silk polysaccharide is measured by a sulfuric acid phenol method, the vitamin content is measured by a nitrophenylhydrazine method, the mineral content is measured by an atomic absorption spectrophotometry, and the amino acid content is measured by an ninhydrin colorimetric method. The humic acid content in biochemical fulvic acid is measured by adopting a sodium pyrophosphate alkali liquor extraction method, the biochemical fulvic acid content is measured by adopting a carbon coefficient conversion algorithm, and the total potassium content is measured by adopting a flame photometer method. The total potassium content in the potassium phosphite is measured by flame photometry, and the phosphorus content is measured by ammonium molybdate spectrophotometry.
The results of the measurements are shown in Table 1 (wherein the water content is calculated based on the total weight of the material and the remaining components are calculated based on the dry matter in the material).
TABLE 1 organic carbon fertilizer composition
Table 2 composition formulation
Example III
The humic acid was replaced with equal weight of biochemical potassium fulvate (details of the components are shown in Table 3) using the formulation of composition A1 in Table 2. Composition A4 was obtained.
TABLE 3 substitution of Biochemical potassium Fulvate Components
Test example 1
And respectively selecting a batch of gentiana macrophylla plants, paris polyphylla plants and polygonatum kingianum plants with basically consistent growth periods, and spraying the liquid composition obtained in the embodiment on the leaf surfaces of the plants in a disease high-rise period (6-9 months) by adopting a spray irrigation method according to the dosage in table 4, wherein the spraying is performed for the first time in 6 months and the spraying is performed for the second time in 8 months. And performing disease investigation on the growth conditions of the gentiana macrophylla, paris polyphylla and polygonatum kingianum plants in 10-11 months, measuring the activity of leaf surface defensive enzymes, chlorophyll content and malondialdehyde content of the plants, and the incidence rate of the gentiana macrophylla leaf rust, paris polyphylla gray mold, paris polyphylla leaf spot and polygonatum kingianum leaf spot, and calculating the control effect.
The method for measuring the activity of the leaf surface defensive enzyme of the plant comprises the following steps: superoxide dismutase (SOD) (U g) -1 ) Polyphenol Oxidase (PPO) (U g) -1 ) And Catalase (CAT) (nmol min -1 g -1 ) All assays were performed using kits purchased from suzhou grissin biotechnology limited, methods of use reference. The method for measuring the chlorophyll content of the leaf surfaces of plants comprises the following steps: and detecting the chlorophyll content in the plant leaves by adopting a SPAD-502 chlorophyll meter, wherein the using method is referred to the instruction book. The content of malondialdehyde in plant leaves is determined by adopting a TBA colorimetric method.
The investigation mode of the prevention and treatment effects of the leaf rust disease of gentiana macrophylla, the gray mold of paris polyphylla, the leaf spot disease of paris polyphylla and the leaf spot disease of polygonatum kingianum comprises the following steps: the classification of each disease was investigated in the following manner, and then the disease index and control effect of each group were calculated in accordance with the following formulas.
Leaf spot and leaf rust grading criteria:
stage 1: leaf lesions are less than 1 cm and less than 5.
2 stages: leaf lesions are more than 5 or greater than 1 cm.
3 stages: leaf lesions are large and numerous.
4 stages: the whole leaf dies.
Gray mold grading standard:
level 0: asymptomatic or symptomatic is not obvious.
Stage 1: forming water stain-like yellow brown lesions with a diameter of 2-5 mm (including 2 mm).
2 stages: forming water stain-like yellow brown lesions with diameters of 5-10 mm (including 5 mm).
3 stages: forming water-stain-shaped yellow brown lesions with the diameter of 10-15 mm (including 10 mm).
4 stages: forming water-stain-shaped yellow brown lesions with the diameters of 15-20 mm (including 15 mm).
5 stages: forming water stain-like yellow brown disease spots with the diameter of more than or equal to 20 mm.
Disease index=100×Σ (number of disease plants at each stage×representative value at each stage)/(total number of investigation×representative value at highest stage)
Control effect (%) = (control disease index-treatment disease index)/control disease index×100
TABLE 4 determination of leaf defensin Activity of plants
Note that: each set of data in the table is an average of the measured data, and "control" refers to a control group to which no fertilizer was applied.
TABLE 5 determination of malondialdehyde and chlorophyll content in plant leaves
Note that: each set of data in the table is an average of the measured data, and "control" refers to a control group to which no fertilizer was applied.
Table 6 determination of the controlling Effect of the composition on diseases
Note that: each set of data in the table is an average of the measured data, and "control" refers to a control group to which no fertilizer was applied.
Test example 2
Culturing Bacillus Siamensis SH-1439 with NB medium (powder medium from Haibo technology Co., ltd., preparation method comprises weighing 18g of culture medium powder with balance, adding 1000 mL deionized water for dissolving, packaging, sterilizing with 121 deg.C high temperature steam for 15 min), diluting the obtained culture solution with fresh medium to obtain viable count of about 1×10 10 cfu/mL of liquid microbial inoculum.
And respectively selecting a batch of gentiana macrophylla, paris polyphylla and Polygonatum kingianum plants with basically consistent growth cycle, processing the same according to the following manner, then investigating the growth conditions of the gentiana macrophylla, paris polyphylla and Polygonatum kingianum plants according to the manner in test example 1, measuring the leaf surface defensive enzyme activity, chlorophyll content and malondialdehyde content of the plants, and the incidence of gentiana macrophylla leaf rust, paris polyphylla gray mold, paris polyphylla leaf spot and Polygonatum kingianum leaf spot, and calculating the control effect. The Siamese bacillus broth was applied as in test example 1.
As can be seen from the comparison of the results in Table 6, the Siamese bacillus provided by the invention has better control effects on leaf rust of gentiana macrophylla, gray mold of paris polyphylla, leaf spot of paris polyphylla and leaf spot of Polygonatum kingianum, has better effect of improving plant growth, and can also improve the chlorophyll content in leaves, improve the activity of leaf defensive enzyme and reduce the malondialdehyde content in leaves. The composition provided by the invention has positive effects on plant growth and disease prevention and control, and has great application value in production practice.
TABLE 7 determination of leaf defensin Activity of plants
Note that: each set of data in the table is an average of the measured data, and "control" refers to a control group to which no fertilizer was applied.
TABLE 8 determination of malondialdehyde and chlorophyll content in plant leaves
Note that: each set of data in the table is an average of the measured data, and "control" refers to a control group to which no fertilizer was applied.
Table 9 determination of the controlling Effect of the composition on diseases
Note that: each set of data in the table is an average of the measured data, and "control" refers to a control group to which no fertilizer was applied.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (10)
1. A microbial composition for controlling fungal diseases of plant leaves, characterized in that: the microbial composition for preventing and treating plant leaf fungal diseases comprises the following components:
component 1: the preservation number is CCTCC NO: m2022925, and/or a microbial inoculum thereof;
component 2: corn silk polysaccharide, biochemical potassium fulvate and polyglutamic acid;
and (3) a component 3: potassium phosphite.
2. The microbial composition for controlling plant foliar fungal diseases according to claim 1 wherein: the amount of component 1 in the composition is 0.1-2 wt% and the amount of component 3 is 3-8 wt% based on the total weight; the weight ratio of the corn silk polysaccharide to the biochemical potassium fulvate to the polyglutamic acid in the component 2 is 1-5:70-80:1-5.
3. The microbial composition for controlling plant foliar fungal diseases according to claim 1 wherein: the microbial inoculum comprises Siamese bacillus and auxiliary materials, wherein the auxiliary materials are methylene dinaphthalene sodium sulfonate (NNO) and polysiloxane in a weight ratio of 0.5-2:1-3, and the whole auxiliary materials account for 2-8% of the total weight of the microbial inoculum.
4. A microbial composition for controlling plant foliar fungal diseases according to claim 2 wherein: the composition contains Siamese bacillus and/or the microbial inoculum in an amount such that the content of Siamese bacillus in the composition is 1×10 10 -3×10 13 cfu/g。
5. The microbial composition for controlling plant foliar fungal diseases according to claim 1 wherein: wherein the moisture content of the corn silk polysaccharide is 30-40 wt%;
and/or the biochemical fulvic acid content in the biochemical fulvic acid potassium is 30-35 wt%, the humic acid content is 2-8 wt% and the K is calculated by dry matter 2 The O content is 5-15 wt%;
and/or the weight average molecular weight of the polyglutamic acid is 1 ten thousand to 1.5 ten thousand.
6. The microbial composition for controlling plant foliar fungal diseases according to claim 1 wherein: the corn silk polysaccharide has sugar content of 90-95 wt% and other substances of 5-10 wt% based on dry matter.
7. Use of a composition according to any one of claims 1 to 6 for reducing malondialdehyde content in plant leaves, increasing plant system resistance, and/or promoting plant growth, and/or controlling plant diseases.
8. A method of promoting plant growth and/or controlling plant disease comprising spraying the composition of any one of claims 1-6 onto the foliage of a plant.
9. The method for promoting plant growth and/or controlling plant diseases according to claim 8, wherein: the plant diseases are leaf rust of gentiana macrophylla, gray mold of paris polyphylla, leaf spot of paris polyphylla and leaf spot of polygonatum kingianum; wherein the pathogen of the leaf rust of the gentiana macrophylla is Ma Geshan rust bacteria, and the pathogen of the gray mold of the paris polyphylla is BotrytisBotrytis sinoviticolaThe pathogens of the paris polyphylla leaf spot are cercospora and Polygonatum kingianum leaf spot are curculigo capitatum; and/or the use amount of the Siamese bacillus, the microbial inoculum or the composition is such that the use amount of the Siamese bacillus applied to the plant leaf surface is not less than 5 multiplied by 10 10 cfu/mu/time, and/or the usage amount of the composition for plant leaf surface is 300-600 g/mu/time.
10. The method for promoting plant growth and/or controlling plant diseases according to claim 9, wherein: the amount of the Siamese bacillus, the microbial inoculum or the composition is such that the amount of the Siamese bacillus applied to the plant leaf surface is 1 multiplied by 10 12 -5×10 13 cfu/mu/time, the application frequency of the composition is 1-3 times per year.
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| CN117694196A (en) * | 2024-01-19 | 2024-03-15 | 云南省烟草公司丽江市公司 | Comprehensive control method for tobacco virus diseases |
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Effective date of registration: 20240315 Address after: 674200 Xincun, Jiexi Village Committee, Qina Town, Yongsheng County, Lijiang City, Yunnan Province Patentee after: Lijiang Wufeng Biotechnology Co.,Ltd. Country or region after: China Address before: No. 2238, Beijing Road, Kunming, Yunnan 650224 Patentee before: INSTITUTE OF AGRICULTURAL ENVIRONMENT AND RESOURCES, YUNNAN ACADEMY OF AGRICULTURAL SCIENCES Country or region before: China |